##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364150_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1238523 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.046313229548424 33.0 31.0 34.0 30.0 34.0 2 31.82636737468743 33.0 31.0 34.0 30.0 34.0 3 32.0701690642806 34.0 31.0 34.0 30.0 34.0 4 35.52880648966551 37.0 35.0 37.0 33.0 37.0 5 35.68516450643226 37.0 35.0 37.0 33.0 37.0 6 35.84274090993869 37.0 35.0 37.0 35.0 37.0 7 35.823371871172355 37.0 35.0 37.0 35.0 37.0 8 35.87758725514181 37.0 35.0 37.0 35.0 37.0 9 37.525666459161435 39.0 37.0 39.0 35.0 39.0 10-11 37.47670451013021 39.0 37.0 39.0 34.0 39.0 12-13 37.458981787177144 39.0 37.0 39.0 34.0 39.0 14-15 38.702650253568166 40.0 38.0 41.0 34.0 41.0 16-17 38.677364489799544 40.0 38.0 41.0 34.0 41.0 18-19 38.75736623381237 40.0 38.0 41.0 34.0 41.0 20-21 38.787001533277945 40.0 38.0 41.0 34.0 41.0 22-23 38.704887192244314 40.0 38.0 41.0 34.0 41.0 24-25 38.6608867982266 40.0 38.0 41.0 34.0 41.0 26-27 38.44040885797034 40.0 38.0 41.0 34.0 41.0 28-29 38.25410347647964 40.0 38.0 41.0 34.0 41.0 30-31 38.05612128317358 40.0 37.0 41.0 34.0 41.0 32-33 37.654167100651335 40.0 36.5 41.0 33.0 41.0 34-35 37.365508755186625 40.0 36.0 41.0 33.0 41.0 36-37 37.22974583435269 40.0 36.0 41.0 32.5 41.0 38-39 37.11462241718563 40.0 36.0 41.0 32.0 41.0 40-41 36.84784174375446 40.0 35.0 41.0 31.0 41.0 42-43 36.648758238643936 40.0 35.0 41.0 31.0 41.0 44-45 36.356327254318245 40.0 35.0 41.0 30.0 41.0 46-47 35.955996780035576 39.0 35.0 41.0 29.5 41.0 48-49 35.56291445536337 39.0 35.0 41.0 27.0 41.0 50-51 35.4023885709026 39.0 35.0 41.0 27.0 41.0 52-53 35.314515354175896 39.0 35.0 41.0 26.5 41.0 54-55 35.1357362761935 39.0 35.0 41.0 26.0 41.0 56-57 34.848129182905765 39.0 35.0 41.0 24.5 41.0 58-59 34.66873162630004 38.0 35.0 40.0 24.0 41.0 60-61 34.47672267693051 38.0 34.5 40.0 24.0 41.0 62-63 34.10845983481938 38.0 34.0 40.0 22.5 41.0 64-65 33.8456169162785 37.0 34.0 40.0 22.0 41.0 66-67 33.37207665905276 36.5 33.5 39.0 22.0 41.0 68-69 33.04751789026122 36.0 33.0 39.0 21.0 41.0 70-71 32.54202990174587 35.0 33.0 39.0 19.5 40.5 72-73 32.2001214349673 35.0 33.0 37.5 18.5 39.5 74-75 31.867511140285647 35.0 32.0 37.0 17.5 39.0 76-77 31.402168954472383 35.0 32.0 36.5 13.0 39.0 78-79 30.97027265541294 35.0 31.5 36.0 9.5 37.0 80-81 30.71176675766215 35.0 31.5 36.0 7.0 37.0 82-83 30.524480772662276 35.0 32.0 35.0 7.0 37.0 84-85 30.27800089299916 35.0 31.0 35.0 2.0 36.0 86-87 29.97335737810279 35.0 31.0 35.0 2.0 36.0 88-89 29.7863200764136 34.5 31.0 35.0 2.0 36.0 90-91 29.590627707357875 34.0 31.0 35.0 2.0 35.5 92-93 29.354008766894115 34.0 30.5 35.0 2.0 35.0 94-95 29.302250341737697 34.0 31.0 35.0 2.0 35.0 96-97 29.18269624383237 34.0 30.0 35.0 2.0 35.0 98-99 29.05485364421977 34.0 30.0 35.0 2.0 35.0 100-101 28.79751849582123 34.0 29.5 35.0 2.0 35.0 102-103 27.368761016145847 32.5 26.0 34.5 2.0 35.0 104-105 28.241095240056097 34.0 29.0 35.0 2.0 35.0 106-107 28.36680667214093 34.0 29.0 35.0 2.0 35.0 108-109 28.249395852963573 34.0 29.0 35.0 2.0 35.0 110-111 28.201783899047495 34.0 29.0 35.0 2.0 35.0 112-113 28.152768257028733 34.0 29.0 35.0 2.0 35.0 114-115 28.022645925832624 34.0 29.0 35.0 2.0 35.0 116-117 27.73904562127631 34.0 29.0 35.0 2.0 35.0 118-119 27.570583671033965 34.0 29.0 35.0 2.0 35.0 120-121 27.33367325435216 34.0 27.5 35.0 2.0 35.0 122-123 26.94452989569027 34.0 26.0 35.0 2.0 35.0 124-125 25.93152004444003 33.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3306933201560369 1101 2 0.6921915441264552 1101 3 0.5153205852687677 1101 4 0.636789181249469 1101 5 0.29415831451657937 1101 6 0.26675857771357414 1101 7 0.2745550567468342 1101 8 0.1984496441182273 1101 9 0.3508393835101913 1101 10-11 0.2855699314799054 1101 12-13 0.2770359164673408 1101 14-15 0.4570625327500082 1101 16-17 0.4592866865739964 1101 18-19 0.3732151830527144 1101 20-21 0.28459327409019153 1101 22-23 0.3030803506429649 1101 24-25 0.3354391790402005 1101 26-27 0.2816266608972384 1101 28-29 0.38981120041778894 1101 30-31 0.2845779243774942 1101 32-33 0.2653801343609956 1101 34-35 0.41947793901703534 1101 36-37 0.4100122041758567 1101 38-39 0.30287502804663546 1101 40-41 0.3634092744291664 1101 42-43 0.408912791610085 1101 44-45 0.31096446295637037 1101 46-47 0.22694163133463263 1101 48-49 0.14144497218835994 1101 50-51 0.1738197739087184 1101 52-53 0.07583386475595688 1101 54-55 0.02039701780758918 1101 56-57 0.14726633956880875 1101 58-59 0.1425803254468434 1101 60-61 0.06074619463351638 1101 62-63 0.15791930511345953 1101 64-65 0.16627660189048754 1101 66-67 0.0680966838206416 1101 68-69 0.1594734056274092 1101 70-71 0.18795650644911177 1101 72-73 0.19295837361629964 1101 74-75 0.1701907296197973 1101 76-77 0.1547321924890923 1101 78-79 0.10077232361367905 1101 80-81 0.08784892461908456 1101 82-83 0.1198187749877313 1101 84-85 0.11402722364869078 1101 86-87 0.17537046898062414 1101 88-89 0.19777755293457489 1101 90-91 0.10093853820692189 1101 92-93 0.11201490891512833 1101 94-95 0.08773090324454103 1101 96-97 0.10938874280660116 1101 98-99 0.258445412794746 1101 100-101 0.25331583877068553 1101 102-103 0.16892502480607163 1101 104-105 0.35591462746104696 1101 106-107 0.23966977560558078 1101 108-109 0.1971402693181048 1101 110-111 0.32768344083992673 1101 112-113 0.3685826238211405 1101 114-115 0.403489465882199 1101 116-117 0.38843085553956413 1101 118-119 0.3369956129609726 1101 120-121 0.37696412716866945 1101 122-123 0.3657804158876026 1101 124-125 0.3915278339250641 1102 1 0.33397720086173877 1102 2 0.6041108999344011 1102 3 0.4982048152181875 1102 4 0.5739011572218757 1102 5 0.37159315240221247 1102 6 0.2929807245255489 1102 7 0.2375418799840361 1102 8 0.2762538743975611 1102 9 0.2699586507110965 1102 10-11 0.30905713117920897 1102 12-13 0.2874775848719793 1102 14-15 0.4376407113803964 1102 16-17 0.49634508002933586 1102 18-19 0.4442517853025336 1102 20-21 0.3878262234203902 1102 22-23 0.27535235541265735 1102 24-25 0.3742642637883691 1102 26-27 0.33965204940730587 1102 28-29 0.4077221656571979 1102 30-31 0.41631535885010607 1102 32-33 0.3540686264034463 1102 34-35 0.4356375306705331 1102 36-37 0.41742862546464465 1102 38-39 0.42754422449151264 1102 40-41 0.30607833772782556 1102 42-43 0.44198151155644894 1102 44-45 0.5596425743066717 1102 46-47 0.4911832693395155 1102 48-49 0.5750998595384118 1102 50-51 0.7241013251446731 1102 52-53 0.7091310541923903 1102 54-55 0.7556021749260253 1102 56-57 0.8920396139407529 1102 58-59 0.8563745685812663 1102 60-61 0.8383326483604918 1102 62-63 0.7671729969753969 1102 64-65 0.6510303285118368 1102 66-67 0.5806602964900378 1102 68-69 0.6934566221337732 1102 70-71 0.7280022946099436 1102 72-73 0.7720152682929253 1102 74-75 0.7601962498857162 1102 76-77 0.7121230744473053 1102 78-79 0.5653433512315047 1102 80-81 0.6026396435026342 1102 82-83 0.5148572813463481 1102 84-85 0.5608332460997367 1102 86-87 0.6076594292735962 1102 88-89 0.6079422948724513 1102 90-91 0.6010756611409462 1102 92-93 0.6152526892144365 1102 94-95 0.7248086205762903 1102 96-97 0.5630921519390171 1102 98-99 0.6221618596523193 1102 100-101 0.5736208732296362 1102 102-103 0.5445252138030092 1102 104-105 0.5239450119001425 1102 106-107 0.6594348903528804 1102 108-109 0.6642360674354393 1102 110-111 0.6303726704888781 1102 112-113 0.5918115434161422 1102 114-115 0.4812717506006656 1102 116-117 0.5412800668544016 1102 118-119 0.6741283267519727 1102 120-121 0.47777746794876563 1102 122-123 0.4872903352811129 1102 124-125 0.5859582862333816 1103 1 0.22054358884016523 1103 2 0.5645235358327589 1103 3 0.34530702550897274 1103 4 0.5547119791038213 1103 5 0.23093623339238434 1103 6 0.21880677106092605 1103 7 0.2792465662326862 1103 8 0.19747690473585067 1103 9 0.3675445531985062 1103 10-11 0.2248382903663284 1103 12-13 0.28578399430561774 1103 14-15 0.33389919862748485 1103 16-17 0.3800502780210664 1103 18-19 0.23835266992940518 1103 20-21 0.14420282714424104 1103 22-23 0.15834366587269955 1103 24-25 0.0976393756208509 1103 26-27 0.19049497099722856 1103 28-29 0.17085725098826998 1103 30-31 0.03748448400097715 1103 32-33 0.06942934887543828 1103 34-35 0.31022322359262944 1103 36-37 0.3751400652971313 1103 38-39 0.23670991704553046 1103 40-41 0.13990461460695514 1103 42-43 0.21761764322995703 1103 44-45 0.19620124458206334 1103 46-47 0.22015876207033358 1103 48-49 0.3044066198307078 1103 50-51 0.4431101464792562 1103 52-53 0.4053943204786705 1103 54-55 0.4184793999249763 1103 56-57 0.4762707733950293 1103 58-59 0.36461959852891823 1103 60-61 0.2552238210298512 1103 62-63 0.23821394888876313 1103 64-65 0.3521950783187435 1103 66-67 0.4376094053624797 1103 68-69 0.5117832282026171 1103 70-71 0.3691975234071805 1103 72-73 0.35831061647709816 1103 74-75 0.5037049195377534 1103 76-77 0.48705422363110173 1103 78-79 0.5006758269103493 1103 80-81 0.43939107825331547 1103 82-83 0.45147178623492223 1103 84-85 0.2796727232194165 1103 86-87 0.4291603859719366 1103 88-89 0.3987391120917181 1103 90-91 0.3701461053659969 1103 92-93 0.21028759401063368 1103 94-95 0.340479624394181 1103 96-97 0.35332750626173137 1103 98-99 0.4433367433969977 1103 100-101 0.4085798714480333 1103 102-103 0.23815214241247418 1103 104-105 0.4091471474479391 1103 106-107 0.4929822910008106 1103 108-109 0.47286255969785174 1103 110-111 0.34548241919453204 1103 112-113 0.5007702531111811 1103 114-115 0.4576576571250328 1103 116-117 0.4894755788319749 1103 118-119 0.5450924875200407 1103 120-121 0.42109761786217703 1103 122-123 0.36179469548649834 1103 124-125 0.4411551796798072 1104 1 0.21932918162530513 1104 2 0.5853516071338483 1104 3 0.0639446150333498 1104 4 0.41506113464490824 1104 5 0.21671707508611604 1104 6 0.13984097437028709 1104 7 0.09310529278696578 1104 8 0.10148965571234925 1104 9 0.17950715256861827 1104 10-11 0.10811604067846048 1104 12-13 0.11181851483323157 1104 14-15 0.2146257361484203 1104 16-17 0.21299686445346566 1104 18-19 0.16724700273563542 1104 20-21 0.15737007777179457 1104 22-23 0.15970052203636698 1104 24-25 0.09854314836330502 1104 26-27 0.17911521526725238 1104 28-29 0.365474536150721 1104 30-31 0.36895989599565127 1104 32-33 0.38965787768903226 1104 34-35 0.2837821848884161 1104 36-37 0.3235083066032587 1104 38-39 0.2638461425314418 1104 40-41 0.2726454879985667 1104 42-43 0.2151879310127498 1104 44-45 0.2953780333039333 1104 46-47 0.2697163323940259 1104 48-49 0.49994443643547015 1104 50-51 0.4866525406031741 1104 52-53 0.4945096047720554 1104 54-55 0.5015316260189877 1104 56-57 0.4600055573933446 1104 58-59 0.6304332274218822 1104 60-61 0.4417119397101743 1104 62-63 0.4584175790625622 1104 64-65 0.5131175011000408 1104 66-67 0.5509018075664898 1104 68-69 0.5722252748229266 1104 70-71 0.5753783114730737 1104 72-73 0.4578961977705589 1104 74-75 0.44781675034191437 1104 76-77 0.43810894437729786 1104 78-79 0.5049337337248012 1104 80-81 0.4693732900904628 1104 82-83 0.41120152825867606 1104 84-85 0.45421308594950816 1104 86-87 0.33644736793359087 1104 88-89 0.4164761010561122 1104 90-91 0.35366452257861525 1104 92-93 0.3808693124237763 1104 94-95 0.46037525360555165 1104 96-97 0.47727754378924914 1104 98-99 0.5361266718972004 1104 100-101 0.5637139590026621 1104 102-103 0.3716148369480621 1104 104-105 0.4362612275090392 1104 106-107 0.42579965588678803 1104 108-109 0.4218143628741018 1104 110-111 0.4357555500907502 1104 112-113 0.4234857131315053 1104 114-115 0.35770308517215454 1104 116-117 0.274168780904791 1104 118-119 0.3874436971989077 1104 120-121 0.37403619603013993 1104 122-123 0.41938985796808126 1104 124-125 0.5931886084978224 1105 1 0.15456038235607394 1105 2 0.44801945954846545 1105 3 0.07626821155175634 1105 4 0.3480710699787224 1105 5 0.07213965875578765 1105 6 0.07850869801590221 1105 7 0.06488475976375696 1105 8 -0.017337982945718977 1105 9 -0.030915491461527722 1105 10-11 0.011051370467960453 1105 12-13 0.06697571362441579 1105 14-15 0.15721387249870133 1105 16-17 0.14231978091054742 1105 18-19 0.04861955964550191 1105 20-21 0.039329137275153414 1105 22-23 -0.0170369541715516 1105 24-25 -0.041997158348380026 1105 26-27 0.024870063219218252 1105 28-29 0.04232569124896912 1105 30-31 0.03572481609851508 1105 32-33 0.017238790902744938 1105 34-35 0.061366920932066193 1105 36-37 -0.1154129310278762 1105 38-39 -0.12231802990635288 1105 40-41 -0.11679424609342703 1105 42-43 -0.1205350063692947 1105 44-45 0.03255545687751038 1105 46-47 0.08345799761874417 1105 48-49 -0.046158892645806304 1105 50-51 -0.04161326277459665 1105 52-53 -0.15266632990985585 1105 54-55 -0.06749258657865198 1105 56-57 0.038176393627331606 1105 58-59 -0.030602477809765105 1105 60-61 -0.08228537223677534 1105 62-63 -0.16823881291030318 1105 64-65 -0.2489787376376995 1105 66-67 -0.10160295085077564 1105 68-69 -0.03681088914831321 1105 70-71 -0.0765208302010052 1105 72-73 -0.11190565891757842 1105 74-75 -0.08050337746132641 1105 76-77 -0.11753737544468024 1105 78-79 -0.0597847651504857 1105 80-81 -0.04911914674230289 1105 82-83 -0.008845995553532049 1105 84-85 -0.04594988732690197 1105 86-87 0.11730062357629123 1105 88-89 0.14198383323267194 1105 90-91 0.09290828633577419 1105 92-93 0.06725541019969228 1105 94-95 0.08306193848766696 1105 96-97 0.21424068768459392 1105 98-99 0.1831124710899772 1105 100-101 0.1269375301696698 1105 102-103 -0.01484409579829915 1105 104-105 4.334641059955402E-4 1105 106-107 0.012180522421733286 1105 108-109 0.16677929343943987 1105 110-111 0.22766785034788484 1105 112-113 0.0987544178360018 1105 114-115 0.13156156803179186 1105 116-117 0.24925197775639063 1105 118-119 0.18166377953059154 1105 120-121 0.11406464593295951 1105 122-123 0.13462524369161954 1105 124-125 0.210822115803456 1106 1 0.14837112471235514 1106 2 0.4598108326106889 1106 3 0.34944239787849085 1106 4 0.46393700210433053 1106 5 0.19093116967111712 1106 6 0.09203190066609324 1106 7 0.027418046488342895 1106 8 0.051072618784402835 1106 9 0.18631913265146238 1106 10-11 0.18630625872030038 1106 12-13 0.1419142063434009 1106 14-15 0.1287117437748364 1106 16-17 0.12091295400897906 1106 18-19 0.08063440938428812 1106 20-21 0.0037777022030240914 1106 22-23 0.11698105845601248 1106 24-25 0.07554847516128405 1106 26-27 0.16866378058409737 1106 28-29 0.2920153884494283 1106 30-31 0.2457130865860293 1106 32-33 0.23920948232004235 1106 34-35 0.2534708179085996 1106 36-37 0.2333275529085057 1106 38-39 0.3446392215001097 1106 40-41 0.32748883645124494 1106 42-43 0.26920277676617843 1106 44-45 0.38697791504096557 1106 46-47 0.24583868602567094 1106 48-49 0.19687985900769434 1106 50-51 0.156830277047753 1106 52-53 0.03687728566503523 1106 54-55 0.10473619944214363 1106 56-57 0.20189828339338334 1106 58-59 0.277928496075738 1106 60-61 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2213 114-115 0.322258804841411 2213 116-117 0.2642216500570882 2213 118-119 0.266861860444493 2213 120-121 0.3195985001865118 2213 122-123 0.20782076226524993 2213 124-125 0.3037874241183829 2214 1 0.11178362060598346 2214 2 0.4151440487245317 2214 3 0.3034241421735189 2214 4 0.40249847264751537 2214 5 0.2050484847142684 2214 6 0.05867211906866743 2214 7 -0.025884809776428597 2214 8 0.0810608623586333 2214 9 -0.25902383041110255 2214 10-11 -0.32441181411241615 2214 12-13 -0.13438088437611384 2214 14-15 -0.040468985537280844 2214 16-17 -0.08486463726831772 2214 18-19 -0.10303844184200983 2214 20-21 0.0083908803338133 2214 22-23 -0.16966160776541273 2214 24-25 -0.05261441954950641 2214 26-27 -0.06980807614806395 2214 28-29 -0.1318318341627034 2214 30-31 -0.058636495437816905 2214 32-33 -0.20461332120580522 2214 34-35 -0.1536012115006855 2214 36-37 -0.10379103431093029 2214 38-39 -0.08947726611131657 2214 40-41 -0.08657831554352668 2214 42-43 -0.17567241648592358 2214 44-45 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-0.5223251966687243 2216 32-33 -0.6984255732724378 2216 34-35 -0.5806109755258859 2216 36-37 -0.3348254077447166 2216 38-39 -0.5265040374858145 2216 40-41 -0.5743520031416267 2216 42-43 -0.5522143046838792 2216 44-45 -0.4721047018688367 2216 46-47 -0.537601969830682 2216 48-49 -0.6433755176093001 2216 50-51 -0.7101073919620973 2216 52-53 -0.7374200868395562 2216 54-55 -0.9271395148944919 2216 56-57 -0.9347748176612569 2216 58-59 -1.0062203485545496 2216 60-61 -0.9770811129175101 2216 62-63 -0.9824055779371932 2216 64-65 -1.0375862248340155 2216 66-67 -0.9324890346574506 2216 68-69 -0.8727952853756022 2216 70-71 -0.8898369164012436 2216 72-73 -0.5618952429219988 2216 74-75 -0.5825848131476086 2216 76-77 -0.5585735530423435 2216 78-79 -0.631877679593071 2216 80-81 -0.6181208852495566 2216 82-83 -0.5926619601946364 2216 84-85 -0.6428313429517054 2216 86-87 -0.7130111697289792 2216 88-89 -0.8896028497171216 2216 90-91 -0.8296544294787154 2216 92-93 -0.8786327873722257 2216 94-95 -0.9092525615657081 2216 96-97 -1.0191734264361614 2216 98-99 -1.0204520565904716 2216 100-101 -1.2216355254602078 2216 102-103 -1.1826906933416304 2216 104-105 -1.223121898438837 2216 106-107 -1.376086998905194 2216 108-109 -1.2908679283112292 2216 110-111 -1.329525487929324 2216 112-113 -1.450565438813566 2216 114-115 -1.4540657923198026 2216 116-117 -1.696538408209154 2216 118-119 -1.8527557955814338 2216 120-121 -1.833305891236158 2216 122-123 -1.84626352052927 2216 124-125 -2.1520536535812305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56.0 3 25.0 4 58.0 5 247.0 6 716.0 7 1261.0 8 2092.0 9 4004.0 10 8531.0 11 10635.0 12 8803.0 13 10729.0 14 15075.0 15 14592.0 16 8523.0 17 7739.0 18 7830.0 19 7812.0 20 7869.0 21 7578.0 22 8179.0 23 8707.0 24 9451.0 25 10996.0 26 12800.0 27 15658.0 28 18790.0 29 22718.0 30 28204.0 31 35276.0 32 45474.0 33 60452.0 34 85986.0 35 135116.0 36 199307.0 37 293494.0 38 123036.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.410812458609705 17.317496601851857 11.079944012426589 22.191746927111843 2 12.602036944034905 23.817582570192535 37.03034746254003 26.550033023232526 3 22.758769365918567 30.863711086493833 28.412228828694985 17.96529071889261 4 13.373267997445346 19.120436196986248 33.4245710414744 34.081724764094005 5 10.81473658543281 39.38626896714877 34.88300176904264 14.915992678375774 6 30.00670992996965 29.5376607940649 21.06425464466843 19.39137463129702 7 21.955829645472875 33.16143503188879 25.06784290642968 19.814892416208664 8 27.82830839637213 31.107698444033737 24.306613603461543 16.757379556132587 9 29.611400030520223 14.319556439404032 22.55759481253073 33.51144871754501 10-11 23.174185370067963 27.67745438617385 26.842225312822332 22.306134930935855 12-13 27.4772963453717 25.63912190734115 26.948695029379298 19.934886717907847 14-15 27.753456379334153 22.680493529709562 27.82115886305043 21.744891227905857 16-17 21.510449173569402 26.631686716150497 33.468562048704946 18.389302061575155 18-19 23.571746346252752 23.999715790502073 34.88647364643208 17.542064216813092 20-21 20.449204596606034 24.317160164631648 35.833139890474335 19.40049534828798 22-23 19.257010164526616 26.35300272986452 30.47299081244353 23.916996293165326 24-25 20.36320681973609 27.69815336493549 29.325737188570578 22.61290262675784 26-27 22.37019417483567 23.122864896332164 30.639196849796086 23.867744079036076 28-29 21.5990511270692 22.388823129504363 34.169275959571166 21.84284978385527 30-31 24.236287900991744 24.404350989040978 32.44824682303034 18.91111428693694 32-33 18.426101089765794 24.773863707012303 34.677272848384725 22.12276235483717 34-35 16.569615582431656 26.24497082411873 35.55610190525327 21.629311688196342 36-37 22.756057013071214 24.471770003463803 31.65669914890559 21.11547383455939 38-39 24.659937683837928 22.768208583934253 31.4639292124571 21.107924519770727 40-41 20.799371509451177 25.75309461350334 31.48346861543952 21.96406526160596 42-43 24.057970663443474 24.586705293321156 30.58728017162378 20.76804387161159 44-45 25.7875307927265 22.135761709713908 30.74004277675909 21.336664720800503 46-47 21.69055399051935 24.379280804635844 35.6036181806878 18.326547024157 48-49 24.03935978580939 24.0783578504396 33.455131636634924 18.42715072711609 50-51 20.320171688373975 25.137361195553094 33.63974669828497 20.90272041778796 52-53 19.393140054726477 27.076768053560574 28.464307889316547 25.065784002396402 54-55 20.547821881386135 28.05507043470327 27.31652944676845 24.080578237142145 56-57 22.683833889237423 23.838636827899037 28.32934874846894 25.148180534394598 58-59 22.175486446355862 23.090729845146193 31.590208659831102 23.14357504866684 60-61 24.522273708279943 25.05791172226919 30.059433696427114 20.360380873023757 62-63 19.03396222758883 25.431259653635824 32.304123540701305 23.230654578074045 64-65 17.114377367235004 26.49098967076106 33.53869891798538 22.85593404401856 66-67 22.87309157762916 25.135504144856412 29.828957556702623 22.162446720811808 68-69 24.603055413585377 23.494719113007996 29.75342403814867 22.148801435257965 70-71 21.048942974817585 26.116511360709488 29.843087290264293 22.991458374208634 72-73 23.985061238265256 25.11592437120667 29.05428482151724 21.84472956901083 74-75 25.568479551853297 22.603334778603223 29.399978845770324 22.428206823773156 76-77 21.60335334911019 24.70051020449358 34.30323861567367 19.392897830722564 78-79 23.59673578932325 24.43555751487861 32.55296833405597 19.414738361742174 80-81 19.92797065536934 25.488061182553736 32.84525196544594 21.738716196630985 82-83 19.19306302749323 27.089646296435347 27.918375355160947 25.79891532091047 84-85 20.353921566252705 27.829035068383874 26.9308684618695 24.88617490349392 86-87 22.211618193606416 23.66657704378522 28.148286305542975 25.973518457065392 88-89 21.66233489406333 23.083220901024852 31.069951870090424 24.184492334821396 90-91 23.734440135548553 24.982499315717188 29.534009461269594 21.749051087464665 92-93 18.841999704486714 25.253628717431976 31.344593519861963 24.559778058219347 94-95 17.093892875526084 26.264409927231252 32.604515497421296 24.037181699821357 96-97 22.33608815871864 24.882326794609742 29.37492203229165 23.40666301437997 98-99 23.677754133021338 23.53741974475775 29.17707769381669 23.60774842840423 100-101 20.56590794034507 25.56432137311943 29.502035892752897 24.367734793782596 102-103 23.040383116177914 24.707553762670315 28.98180060889376 23.270262512258014 104-105 24.47274506906101 22.44072899480155 29.36144713560153 23.72507880053592 106-107 21.051199246720756 24.36698459766829 33.5759202596423 21.00589589596865 108-109 22.800212713637293 24.485245515065397 31.509802472695085 21.204739298602217 110-111 19.353803242733406 25.20042993108505 32.357522744194235 23.08824408198731 112-113 18.61785801383458 26.514399045178006 27.95406130435274 26.913681636634674 114-115 19.52072664415761 27.415429476098936 27.063052293177815 26.000791586565647 116-117 21.257054560111275 23.79075934408645 28.035328736241233 26.916857359561043 118-119 20.70250006258838 23.43930824898708 30.38827408703742 25.46991760138712 120-121 22.451666668685476 24.935246680370344 29.208259192548812 23.40482745839536 122-123 18.30358674821224 25.443453497183466 30.48221240470751 25.770747349896787 124-125 16.842255700049577 26.410781446558655 31.50307872923493 25.24388412415684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2566.0 1 2059.5 2 5758.5 3 7653.0 4 3540.5 5 1646.5 6 2105.5 7 4440.5 8 6679.5 9 6665.5 10 4902.5 11 2901.5 12 2198.0 13 2170.0 14 2438.0 15 2884.0 16 3000.5 17 3242.0 18 3607.5 19 3536.0 20 3499.5 21 3859.0 22 4268.5 23 4721.5 24 5183.5 25 5657.5 26 6459.0 27 7354.5 28 8038.5 29 8966.5 30 10215.0 31 11603.0 32 13051.0 33 14399.5 34 16047.0 35 17354.5 36 18621.5 37 20352.0 38 20812.5 39 20459.5 40 20086.0 41 20462.5 42 20964.0 43 21368.5 44 22450.5 45 23869.5 46 25977.5 47 32382.5 48 42570.5 49 77150.0 50 194694.5 51 197104.0 52 72380.0 53 28083.5 54 21295.0 55 18526.0 56 16264.5 57 14085.5 58 12854.5 59 11799.0 60 10481.0 61 9162.0 62 7670.5 63 5954.0 64 4469.5 65 3388.0 66 2313.5 67 1792.5 68 1339.0 69 765.5 70 590.5 71 524.5 72 524.5 73 502.0 74 370.5 75 290.5 76 183.0 77 91.5 78 52.5 79 21.5 80 12.5 81 8.5 82 5.0 83 2.0 84 2.0 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1469492290413662 2 8.07413346381133E-5 3 5.651893424667931E-4 4 0.0 5 0.0 6 0.004844480078286798 7 0.0 8 0.0 9 0.0 10-11 4.037066731905665E-5 12-13 0.005328928086115478 14-15 0.0012918613542098128 16-17 3.229653385524532E-4 18-19 0.0 20-21 4.037066731905665E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 8.07413346381133E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.477840137001897E-4 96-97 0.0023818693718243426 98-99 0.004238920068500948 100-101 0.0 102-103 0.0038352133953103817 104-105 0.02180016035229059 106-107 0.016471232266175112 108-109 0.019256808311190022 110-111 0.01493714690805096 112-113 0.014291216230946055 114-115 0.04061289132297099 116-117 0.03988621931122797 118-119 0.022123125690843043 120-121 0.013685656221160204 122-123 0.0027048347103767953 124-125 0.0020185333659528326 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1238523.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.875604886365025 #Duplication Level Percentage of deduplicated Percentage of total 1 86.5614111822503 41.44179920167594 2 8.850940027570422 8.474882152657486 3 2.1516584631550946 3.090358512972501 4 0.8134153533047747 1.5577100825328962 5 0.40094393769153264 0.9597716771251588 6 0.2543989352671453 0.7307701745017081 7 0.1738924896980888 0.5827645690644401 8 0.11097683816001555 0.42504666042295847 9 0.08026879429750702 0.34586253724431204 >10 0.5189586388583217 4.664074554676756 >50 0.04139197479453349 1.387065490283669 >100 0.03095376643581007 3.0441388547593515 >500 0.0037144519483153144 1.1877459506367043 >1k 0.0028300586272878587 3.4512039225865454 >5k 0.0024763012988768766 8.233350601921778 >10k+ 0.001768786642054912 20.423455056937808 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 59398 4.795873794834654 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 49652 4.008968747451601 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31445 2.5389112676954726 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 17897 1.4450276660183137 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 17855 1.441636529963513 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15975 1.28984282084386 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15005 1.2115237262448901 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11794 0.9522633007219082 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11350 0.916414148142586 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10808 0.8726523447687284 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9381 0.7574344602401408 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8469 0.6837983630501815 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8061 0.6508558985178313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7832 0.6323661328857034 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7569 0.6111311618758796 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7452 0.6016844257232203 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7138 0.5763316466468527 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6624 0.5348306006428625 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6438 0.5198127124001735 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 6287 0.5076207708698184 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5780 0.46668491420829483 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5653 0.4564307647092545 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5461 0.4409284284587367 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5082 0.41032746263089176 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4740 0.38271392618465705 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4593 0.3708449499928544 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3884 0.31359934373443205 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3402 0.2746820204388615 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3166 0.2556270654642667 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 3111 0.2511862920591705 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3005 0.24262771058753047 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2456 0.19830071787120626 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2275 0.18368653630170775 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2171 0.17528943749934398 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1925 0.1554270691783681 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1432 0.11562159120177824 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0185333659528324E-4 0.0 12-13 0.0 0.0 0.0 6.459306771049064E-4 0.0 14-15 0.0 0.0 0.0 0.0017359386947194359 0.0 16-17 0.0 0.0 0.0 0.003592989391396042 0.0 18-19 0.0 0.0 0.0 0.005369298753434535 0.0 20-21 0.0 0.0 0.0 0.00722634945011114 0.0 22-23 0.0 0.0 0.0 0.011707493522526428 0.0 24-25 0.0 0.0 0.0 0.01699605094132285 0.0 26-27 0.0 0.0 0.0 0.02135608301178097 0.0 28-29 0.0 0.0 0.0 0.030923931166397394 0.0 30-31 0.0 0.0 0.0 0.044811440724152884 0.0 32-33 0.0 0.0 0.0 0.0667730837457197 0.0 34-35 4.037066731905665E-5 0.0 0.0 0.10153222830742747 0.0 36-37 8.07413346381133E-5 0.0 0.0 0.14844294373217132 0.0 38-39 1.614826692762266E-4 0.0 0.0 0.22353238494561667 0.0 40-41 1.614826692762266E-4 0.0 0.0 0.33891175214348057 0.0 42-43 3.229653385524532E-4 0.0 0.0 0.42966501227671994 0.0 44-45 4.037066731905665E-4 0.0 0.0 0.5162197230087774 0.0 46-47 6.86301344423963E-4 0.0 0.0 0.6312357542007698 0.0 48-49 8.07413346381133E-4 0.0 0.0 0.7332120598487069 0.0 50-51 8.07413346381133E-4 0.0 0.0 0.8398309922383356 0.0 52-53 8.07413346381133E-4 0.0 0.0 0.9788271998178475 0.0 54-55 8.07413346381133E-4 0.0 0.0 1.1208108367789698 0.0 56-57 8.07413346381133E-4 0.0 0.0 1.278377551325248 0.0 58-59 8.07413346381133E-4 0.0 0.0 1.4639615089909512 0.0 60-61 8.07413346381133E-4 0.0 0.0 1.638322421141957 0.0 62-63 8.07413346381133E-4 0.0 0.0 1.8046495704964705 0.0 64-65 8.07413346381133E-4 0.0 0.0 2.0025869523618054 0.0 66-67 8.881546810192464E-4 0.0 0.0 2.2220015292408783 0.0 68-69 9.688960156573596E-4 0.0 0.0 2.4972891096895253 0.0 70-71 9.688960156573596E-4 0.0 0.0 2.9120169750581946 0.0 72-73 0.0010092666829764163 0.0 0.0 3.2380908549942147 0.0 74-75 0.001049637350295473 0.0 0.0 3.5384486198479963 0.0 76-77 0.001049637350295473 0.0 0.0 3.8977071883202816 0.0 78-79 0.001049637350295473 0.0 0.0 4.233671881749471 0.0 80-81 0.0011303786849335862 0.0 0.0 4.60173125569731 0.0 82-83 0.0011303786849335862 0.0 0.0 5.074471770003464 0.0 84-85 0.0011707493522526429 0.0 0.0 5.519881342534616 0.0 86-87 0.0012111200195716995 0.0 0.0 5.966703888421935 0.0 88-89 0.0012111200195716995 0.0 0.0 6.48518436880058 0.0 90-91 0.0012111200195716995 0.0 0.0 6.97003608330245 0.0 92-93 0.0012111200195716995 0.0 0.0 7.447701819021528 0.0 94-95 0.0012111200195716995 0.0 0.0 8.007885198740759 0.0 96-97 0.0012111200195716995 0.0 0.0 8.558702583641967 0.0 98-99 0.0013322320215288694 0.0 0.0 9.176050828284982 0.0 100-101 0.001493714690805096 0.0 0.0 10.096744267163388 0.0 102-103 0.001614826692762266 0.0 0.0 10.823335537571769 0.0 104-105 0.0018974213639956627 0.0 0.0 11.497606423134652 0.0 106-107 0.001978162698633776 0.0 0.0 12.2247628828855 0.0 108-109 0.0021396453679100027 0.0 0.0 12.884177362874972 0.0 110-111 0.002180016035229059 0.0 0.0 13.58145952880972 0.0 112-113 0.002180016035229059 0.0 0.0 14.43126207587586 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 85 1.4551915E-11 62.999123 2 TATAACG 140 0.0 59.499176 2 GCGTATC 45 3.1498077E-4 52.960854 1 CGTATAC 45 3.1712445E-4 52.888153 3 ATCACGC 105 1.1277734E-10 50.999294 3 AGTACTC 1380 0.0 50.876102 5 AAAGTAC 1460 0.0 46.458256 3 AAGTACT 1580 0.0 45.189247 4 ATAACGC 200 0.0 44.62438 3 AAAAGTA 1655 0.0 40.624813 2 GCAACGC 60 0.0013086408 39.666115 3 TCACGCA 140 1.893568E-9 38.24947 4 TAACGCA 255 0.0 34.99951 4 AAAAAGT 1945 0.0 34.61516 1 GTATAAC 315 0.0 32.154804 1 GTTATCA 795 0.0 31.476734 1 CGTATCA 80 0.0053627263 29.749586 2 TTATCAA 820 0.0 29.023985 2 GGTATAC 105 5.8591185E-4 28.371887 1 CGACGAG 55 7.736135E-4 27.056002 114-115 >>END_MODULE