##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364149_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1274463 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.231132641747937 31.0 31.0 34.0 26.0 34.0 2 30.30188714776341 31.0 31.0 34.0 26.0 34.0 3 30.438011146655494 33.0 31.0 34.0 26.0 34.0 4 33.669712655447825 37.0 35.0 37.0 28.0 37.0 5 33.76417989380625 37.0 35.0 37.0 30.0 37.0 6 33.55535390199637 37.0 35.0 37.0 27.0 37.0 7 33.514779950457566 37.0 35.0 37.0 28.0 37.0 8 33.36827040094534 37.0 35.0 37.0 26.0 37.0 9 34.65430459730883 39.0 35.0 39.0 25.0 39.0 10-11 34.65770249901331 39.0 35.0 39.0 25.5 39.0 12-13 34.52987885878209 39.0 35.0 39.0 24.0 39.0 14-15 35.674927793117575 40.0 36.0 41.0 24.5 41.0 16-17 35.730494726014015 40.0 36.0 41.0 25.0 41.0 18-19 35.659191753703325 40.0 36.0 41.0 25.0 41.0 20-21 35.69267212935958 40.0 36.0 41.0 25.0 41.0 22-23 35.72835696289339 40.0 36.0 41.0 25.5 41.0 24-25 35.742010948925156 40.0 36.0 41.0 25.5 41.0 26-27 35.55523973626539 40.0 36.0 41.0 25.0 41.0 28-29 35.44973216170261 40.0 35.0 41.0 25.0 41.0 30-31 35.525364800704295 40.0 35.0 41.0 25.0 41.0 32-33 35.20336957604889 39.0 35.0 41.0 25.0 41.0 34-35 35.26632511104677 39.0 35.0 41.0 25.0 41.0 36-37 35.262915439679304 40.0 35.0 41.0 25.0 41.0 38-39 35.11451372068079 40.0 35.0 41.0 25.0 41.0 40-41 34.75246397894642 39.0 34.0 41.0 24.0 41.0 42-43 34.50126366948275 39.0 33.0 41.0 23.5 41.0 44-45 34.23215738707205 38.0 33.0 41.0 22.0 41.0 46-47 33.94984789672199 38.0 33.0 40.0 20.5 41.0 48-49 33.64004055041221 38.0 33.0 40.0 16.5 41.0 50-51 33.539315774565445 38.0 33.0 40.0 16.0 41.0 52-53 33.363999582569285 38.0 32.5 40.0 13.5 41.0 54-55 33.05018348904598 38.0 32.0 40.0 8.5 41.0 56-57 32.6439002152279 37.5 31.0 40.0 7.5 41.0 58-59 32.2038356547032 37.0 31.0 40.0 4.0 41.0 60-61 31.872210491791446 36.0 30.0 40.0 2.0 41.0 62-63 31.290803263806012 36.0 29.5 39.0 2.0 41.0 64-65 31.029853750167717 36.0 29.0 39.0 2.0 41.0 66-67 30.544914210926486 35.0 29.5 38.5 2.0 40.5 68-69 30.15372670685614 35.0 29.0 38.0 2.0 40.0 70-71 29.51902056003195 34.5 28.0 37.0 2.0 39.5 72-73 29.276707130768017 34.0 28.0 37.0 2.0 39.0 74-75 28.943317303052346 34.0 28.0 36.0 2.0 39.0 76-77 28.202981569492408 34.0 26.0 36.0 2.0 38.0 78-79 27.724124592083097 34.0 26.0 35.0 2.0 37.0 80-81 27.610892587701642 34.0 26.0 35.0 2.0 37.0 82-83 27.427584009892794 34.0 26.0 35.0 2.0 36.0 84-85 27.151655638492446 34.0 26.0 35.0 2.0 36.0 86-87 26.80936441465935 33.0 25.0 35.0 2.0 36.0 88-89 26.42969941065374 33.0 24.0 35.0 2.0 35.5 90-91 26.24245427289768 33.0 24.0 35.0 2.0 35.0 92-93 25.89453126532508 33.0 21.5 35.0 2.0 35.0 94-95 25.80232066368345 33.0 20.0 35.0 2.0 35.0 96-97 25.716637124812568 33.0 21.5 35.0 2.0 35.0 98-99 25.523163089081443 33.0 20.0 35.0 2.0 35.0 100-101 24.93529745469268 32.0 18.0 35.0 2.0 35.0 102-103 23.667156676969043 30.0 12.0 34.0 2.0 35.0 104-105 24.43131381609352 31.5 14.0 35.0 2.0 35.0 106-107 24.274764351730887 32.0 9.0 35.0 2.0 35.0 108-109 24.147532725547936 32.0 7.0 35.0 2.0 35.0 110-111 24.30568129478847 32.0 7.0 35.0 2.0 35.0 112-113 24.219205265276432 32.0 3.5 35.0 2.0 35.0 114-115 24.023642506687132 32.0 2.0 35.0 2.0 35.0 116-117 23.649122807017545 32.0 2.0 35.0 2.0 35.0 118-119 23.31271680700028 32.0 2.0 35.0 2.0 35.0 120-121 23.066997629589874 32.0 2.0 35.0 2.0 35.0 122-123 22.532404236137104 31.0 2.0 35.0 2.0 35.0 124-125 21.416385567882315 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2814988094150692 1101 2 0.4337385892258574 1101 3 0.42901822122562905 1101 4 0.46114212790377707 1101 5 0.3305704020338567 1101 6 0.38871220632091763 1101 7 0.4723709143996686 1101 8 0.340887321275126 1101 9 0.6124163526892232 1101 10-11 0.39659177561900094 1101 12-13 0.4846021596124146 1101 14-15 0.4574318722734958 1101 16-17 0.6093420556047917 1101 18-19 0.5109608427574415 1101 20-21 0.5378452204209765 1101 22-23 0.5799864014965053 1101 24-25 0.6209212328732221 1101 26-27 0.6154879819165728 1101 28-29 0.7308850205118276 1101 30-31 0.695201835279228 1101 32-33 0.7660434269164611 1101 34-35 0.7061171333898955 1101 36-37 0.7590696804228614 1101 38-39 0.8398823550075178 1101 40-41 0.8506319480347315 1101 42-43 1.0315512452000348 1101 44-45 1.0071482901976836 1101 46-47 1.0672719728160018 1101 48-49 1.0411671070057267 1101 50-51 1.020475815319152 1101 52-53 0.9997378585724306 1101 54-55 1.0809756924730394 1101 56-57 1.027665950787494 1101 58-59 0.9494716057819375 1101 60-61 1.032254892252908 1101 62-63 1.0718429701628125 1101 64-65 0.9620795933054644 1101 66-67 1.0924204045667878 1101 68-69 1.0912172757733494 1101 70-71 0.9063532527045375 1101 72-73 1.0311617501291224 1101 74-75 1.024313150955738 1101 76-77 0.9429081123616214 1101 78-79 1.0795793462097762 1101 80-81 1.0329651074693516 1101 82-83 1.0028413463666475 1101 84-85 0.9634297593055194 1101 86-87 0.9914653373613618 1101 88-89 1.0431297655595415 1101 90-91 0.9702002571972415 1101 92-93 0.880467345492491 1101 94-95 0.9689412246068443 1101 96-97 1.0538738924744884 1101 98-99 1.0689576494267214 1101 100-101 0.946378577420294 1101 102-103 0.9151510998138548 1101 104-105 1.0880296397191067 1101 106-107 0.9558899832140533 1101 108-109 1.0270890581305459 1101 110-111 1.0377754802030132 1101 112-113 1.0744430101219855 1101 114-115 1.058831942470416 1101 116-117 0.9303471648878059 1101 118-119 0.861435666792719 1101 120-121 0.7692956042124557 1101 122-123 0.7806028064337838 1101 124-125 0.8228681060943934 1102 1 0.35256309450914003 1102 2 0.48306005390981 1102 3 0.42222866660808833 1102 4 0.6285295778714257 1102 5 0.446928635069078 1102 6 0.5981906934602605 1102 7 0.5834101027901966 1102 8 0.5020770618771735 1102 9 0.5694119187515341 1102 10-11 0.6497002936530549 1102 12-13 0.5807362589180514 1102 14-15 0.670602739930402 1102 16-17 0.6259539165981849 1102 18-19 0.7156418261224786 1102 20-21 0.6486239184734615 1102 22-23 0.7407554361977375 1102 24-25 0.7408232548944582 1102 26-27 0.8582033556081612 1102 28-29 0.8553156530551931 1102 30-31 0.7674786608823467 1102 32-33 0.882506136184027 1102 34-35 0.827341774095288 1102 36-37 0.7840299766535921 1102 38-39 0.9080023990387787 1102 40-41 0.8236854830006521 1102 42-43 0.8072906316192388 1102 44-45 0.8151012282766104 1102 46-47 0.7590745099358713 1102 48-49 0.7521518165029804 1102 50-51 0.7571179828228409 1102 52-53 0.8625546834472928 1102 54-55 0.9378873694426133 1102 56-57 0.9304732243499672 1102 58-59 0.9686035007231055 1102 60-61 0.9098584651719932 1102 62-63 0.8616802057160982 1102 64-65 0.7819244755106141 1102 66-67 0.865230815217199 1102 68-69 0.743110732424114 1102 70-71 0.6352084396833781 1102 72-73 0.6152308052774487 1102 74-75 0.5030061616918609 1102 76-77 0.44088160957045375 1102 78-79 0.37936507189944635 1102 80-81 0.5332842781577511 1102 82-83 0.5102728836237667 1102 84-85 0.4621894891798348 1102 86-87 0.5506120494411135 1102 88-89 0.5778413635417188 1102 90-91 0.6628290808732942 1102 92-93 0.530323235515155 1102 94-95 0.5246074496536899 1102 96-97 0.6244862408368981 1102 98-99 0.6069406419285173 1102 100-101 0.41155185951452466 1102 102-103 0.33215271597433826 1102 104-105 0.47580241931906997 1102 106-107 0.4725439988266693 1102 108-109 0.36713244517718024 1102 110-111 0.30118331003840026 1102 112-113 0.48987482187429876 1102 114-115 0.48223994036408513 1102 116-117 0.19960342295810563 1102 118-119 0.24940773550614637 1102 120-121 0.4344555816926423 1102 122-123 0.40045693184447373 1102 124-125 0.321091169852906 1103 1 0.4250273074122255 1103 2 0.48253448230273577 1103 3 0.589000374523998 1103 4 0.49258930341234475 1103 5 0.39140136453396934 1103 6 0.376119700190209 1103 7 0.36728754979460376 1103 8 0.40187264247212084 1103 9 0.6631889097855748 1103 10-11 0.6945370595517346 1103 12-13 0.6783902311213978 1103 14-15 0.6462095586602601 1103 16-17 0.6357905728886024 1103 18-19 0.5159485922403633 1103 20-21 0.6927264460399414 1103 22-23 0.6143020306691014 1103 24-25 0.7367589806107517 1103 26-27 0.8369482588240658 1103 28-29 0.8251990024644016 1103 30-31 0.6614750054468743 1103 32-33 0.7621779142492286 1103 34-35 0.6932696354189218 1103 36-37 0.7038523564681327 1103 38-39 0.6710311218833311 1103 40-41 0.9234827077195149 1103 42-43 0.9597946323207367 1103 44-45 1.1004027606000832 1103 46-47 0.9183103759507389 1103 48-49 0.8773608942590769 1103 50-51 0.8632811182767313 1103 52-53 0.906463752434739 1103 54-55 1.039326388887126 1103 56-57 0.861329182654984 1103 58-59 0.9123003407169676 1103 60-61 0.9055403107558817 1103 62-63 0.7979440985630646 1103 64-65 0.7346852335613399 1103 66-67 0.8979529911603805 1103 68-69 0.9398797549376532 1103 70-71 0.9981252250923269 1103 72-73 0.9802694695911924 1103 74-75 0.8740534138127778 1103 76-77 0.862326054623832 1103 78-79 0.7262141084428393 1103 80-81 0.730206341204422 1103 82-83 0.8063678153378184 1103 84-85 0.9125052979791874 1103 86-87 0.8746640750017605 1103 88-89 0.9213083002100646 1103 90-91 0.8785207363964531 1103 92-93 0.6176923906546854 1103 94-95 0.8072622165169214 1103 96-97 0.8318594514820496 1103 98-99 0.8211320343918551 1103 100-101 0.8370598937999887 1103 102-103 0.6881069106824533 1103 104-105 0.7552431979761529 1103 106-107 0.6228150052497128 1103 108-109 0.5632899906097997 1103 110-111 0.8396293663332024 1103 112-113 1.0165494990779713 1103 114-115 1.0136809327572713 1103 116-117 0.8396666121200411 1103 118-119 0.966448942731521 1103 120-121 1.0628438470323225 1103 122-123 0.9805941339840274 1103 124-125 0.8780322499327013 1104 1 0.25259720828205445 1104 2 0.27231073444476905 1104 3 0.3150947108724509 1104 4 0.2533393413724738 1104 5 0.2814197480831311 1104 6 0.26648785923230633 1104 7 0.1955323275651537 1104 8 0.16011920979170213 1104 9 0.4719495657366437 1104 10-11 0.38984315377962275 1104 12-13 0.3080293658473252 1104 14-15 0.37305753918128914 1104 16-17 0.46883771394845297 1104 18-19 0.5084201492564802 1104 20-21 0.458084572761706 1104 22-23 0.41550428224715574 1104 24-25 0.49524795048125014 1104 26-27 0.42095877378035595 1104 28-29 0.5171743663393755 1104 30-31 0.47113301178079325 1104 32-33 0.5418174556908397 1104 34-35 0.5047410514402628 1104 36-37 0.4922541807235703 1104 38-39 0.508650560724135 1104 40-41 0.728540081746111 1104 42-43 0.5485850056315087 1104 44-45 0.4049666529234557 1104 46-47 0.48287269564011126 1104 48-49 0.6874623562664084 1104 50-51 0.6743238559613758 1104 52-53 0.6165936845403266 1104 54-55 0.5973615996200792 1104 56-57 0.6347987115155291 1104 58-59 0.6738110725882933 1104 60-61 0.46593176104980216 1104 62-63 0.664594496602156 1104 64-65 0.6446993872673339 1104 66-67 0.4631568370639414 1104 68-69 0.504573308222831 1104 70-71 0.5971778486796673 1104 72-73 0.49449380854262515 1104 74-75 0.49359284896602773 1104 76-77 0.44145110071827887 1104 78-79 0.6252453116705361 1104 80-81 0.6168602369984377 1104 82-83 0.5560584402700322 1104 84-85 0.5179481029074964 1104 86-87 0.5308991620931423 1104 88-89 0.6861017699829546 1104 90-91 0.6115150044166704 1104 92-93 0.7181306945960344 1104 94-95 0.6037479218161543 1104 96-97 0.6667845344520522 1104 98-99 0.6571129512917047 1104 100-101 0.5427408985504272 1104 102-103 0.21304969934213602 1104 104-105 0.3968095414394206 1104 106-107 0.5694612276743882 1104 108-109 0.6070657492701308 1104 110-111 0.5332982943391045 1104 112-113 0.46788257753614104 1104 114-115 0.3824035472341798 1104 116-117 0.512235265728421 1104 118-119 0.31657705817370285 1104 120-121 0.3145512394966907 1104 122-123 0.2912079221881889 1104 124-125 0.2400069088338057 1105 1 0.2953285604114271 1105 2 0.39175761419874533 1105 3 0.35486818611832405 1105 4 0.35305393219950787 1105 5 0.3112394077224536 1105 6 0.2645779392834342 1105 7 0.45366075349942037 1105 8 0.38340122409766764 1105 9 0.3475171749363781 1105 10-11 0.3989580849127137 1105 12-13 0.5942704587596239 1105 14-15 0.6337147723237138 1105 16-17 0.6835544487582652 1105 18-19 0.5943483845538111 1105 20-21 0.584792862737423 1105 22-23 0.5750211077324181 1105 24-25 0.5493852910139978 1105 26-27 0.665781806437181 1105 28-29 0.8263903514380075 1105 30-31 0.629109173969681 1105 32-33 0.5006137937136259 1105 34-35 0.584586520808621 1105 36-37 0.6361419179614103 1105 38-39 0.7234598828159022 1105 40-41 0.6930839323391851 1105 42-43 0.8166983827184779 1105 44-45 0.7796216355667624 1105 46-47 0.7747330718339782 1105 48-49 0.7654547144016632 1105 50-51 0.7713155886545167 1105 52-53 0.7399984657584966 1105 54-55 0.7874852132006396 1105 56-57 0.6768931505431084 1105 58-59 0.7736257614649844 1105 60-61 0.7620261618407511 1105 62-63 0.7430125104274268 1105 64-65 0.7576968390686893 1105 66-67 0.8736417322272771 1105 68-69 0.7786125444733472 1105 70-71 0.6356874665591192 1105 72-73 0.5770245822063238 1105 74-75 0.6928345345461757 1105 76-77 0.643824912992784 1105 78-79 0.5776316874110314 1105 80-81 0.5461836397312645 1105 82-83 0.5363575600080601 1105 84-85 0.5561839191640772 1105 86-87 0.5770958458843616 1105 88-89 0.4094681780060938 1105 90-91 0.2578375959865866 1105 92-93 0.23695977956602832 1105 94-95 0.3398185885811422 1105 96-97 0.3472072404587543 1105 98-99 0.3446980619457989 1105 100-101 0.419794820400206 1105 102-103 0.1373822652115706 1105 104-105 0.36585671179182455 1105 106-107 0.1533117115043865 1105 108-109 0.1741875684600096 1105 110-111 0.37332495073650307 1105 112-113 0.3448794175513292 1105 114-115 0.3511793740331477 1105 116-117 0.20018499001496792 1105 118-119 0.34229092250465953 1105 120-121 0.2607313995989493 1105 122-123 0.17539406905510901 1105 124-125 0.1002866917589671 1106 1 0.207815015358495 1106 2 0.24988504928332134 1106 3 0.28102687632851087 1106 4 0.22499288467955836 1106 5 0.26954565094992233 1106 6 0.2179660174857858 1106 7 0.29541635368979513 1106 8 0.31340818820428495 1106 9 0.2866395899487344 1106 10-11 0.2975971263935122 1106 12-13 0.4309860892801538 1106 14-15 0.46966784661378824 1106 16-17 0.4616046064589767 1106 18-19 0.40962464985669556 1106 20-21 0.4113145469219148 1106 22-23 0.3587096373917191 1106 24-25 0.4964809358929543 1106 26-27 0.6322977633834057 1106 28-29 0.5688051217972685 1106 30-31 0.2778940852512619 1106 32-33 0.12422198070661494 1106 34-35 0.3845066621342781 1106 36-37 0.28062830837621533 1106 38-39 0.20249173755378536 1106 40-41 0.24842451479662486 1106 42-43 0.1779182575796625 1106 44-45 0.16367181477822612 1106 46-47 0.18754420472473043 1106 48-49 0.18389158168299957 1106 50-51 0.2775019470720821 1106 52-53 0.31261191481968353 1106 54-55 0.3763866052153091 1106 56-57 0.27742419728329537 1106 58-59 0.3380097338021528 1106 60-61 0.3569122489539325 1106 62-63 0.3975351464627792 1106 64-65 0.566402778994533 1106 66-67 0.3724001583929599 1106 68-69 0.2836613873292215 1106 70-71 0.3284277215153324 1106 72-73 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>>Per sequence quality scores pass #Quality Count 2 47056.0 3 7541.0 4 5157.0 5 3781.0 6 4395.0 7 4400.0 8 4968.0 9 6029.0 10 7385.0 11 8679.0 12 9913.0 13 10825.0 14 11500.0 15 11385.0 16 11241.0 17 11217.0 18 11317.0 19 11672.0 20 12375.0 21 12986.0 22 14021.0 23 17002.0 24 19480.0 25 21097.0 26 23916.0 27 25407.0 28 29233.0 29 36436.0 30 42736.0 31 51406.0 32 63498.0 33 77864.0 34 92788.0 35 108356.0 36 141896.0 37 207451.0 38 87494.0 39 560.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.1117611716228 16.62516617281342 9.364261845775859 22.898810809787914 2 12.470140063795803 24.113741325836354 36.411935459965214 27.00418315040263 3 23.13999033587181 32.77432211185637 25.72494756067472 18.360739991597097 4 13.659023009798279 19.51268731426229 31.617622714982772 35.21066696095666 5 11.195817629869389 42.848877036457786 31.960415052307216 13.994890281365608 6 30.526719916247007 28.65962103032577 21.84893558650744 18.964723466919775 7 25.43063969720043 34.04319717757001 21.515274762866383 19.010888362363186 8 30.1913524764653 32.07814939211883 22.155652269896084 15.574845861519782 9 32.96188589740355 16.449244278468875 18.313157629128714 32.27571219499886 10-11 23.468219806874714 31.55845113376633 23.076662246809406 21.896666812549554 12-13 27.51292043660588 28.306441056471414 22.68571599800023 21.494922508922475 14-15 28.916193612397578 26.168924621277373 22.15967389903279 22.755207867292263 16-17 23.468191094286905 29.44590887571492 29.063855904693312 18.02204412530486 18-19 25.788208084648346 28.235690110946056 28.355389982338654 17.620711822066948 20-21 22.011417660676397 29.26300785944681 28.714592776277943 20.010981703598855 22-23 20.35682014227389 31.813519015846936 21.775182967503458 26.05447787437572 24-25 21.26602796576496 33.255072500331124 20.61671008066805 24.862189453235867 26-27 24.289754583716395 27.431849397694858 22.009171375091263 26.269224643497484 28-29 23.80142177236268 27.497414941421766 25.580154949771217 23.12100833644434 30-31 26.740469897302617 29.766510118341706 23.780941687063155 19.712078297292525 32-33 19.29922433920592 30.37535611243888 27.056581367897564 23.26883818045764 34-35 16.998285856661663 31.920084966000985 27.849467622087374 23.232161555249977 36-37 24.664007758411085 28.932985975513404 24.594572974106185 21.80843329196933 38-39 27.748284396467206 26.74714637256358 23.65116199370443 21.85340723726479 40-41 21.92115956369045 31.722211105631594 23.549796986546124 22.806832344131834 42-43 25.79317261561721 29.247478207227612 23.00648672028476 21.95286245687042 44-45 27.92052677413889 26.852161988564493 23.19165704513124 22.035654192165378 46-47 23.042204166111663 28.724282981585976 29.661220671190243 18.572292181112125 48-49 25.52547298183972 27.964380109955545 28.067273782684303 18.44287312552043 50-51 20.73585605975235 29.6314888204695 28.61322283960784 21.01943228017031 52-53 19.565034096586245 31.66053605790825 22.350836300138212 26.42359354536729 54-55 20.65070152835073 32.82660173441606 21.104439269827385 25.418257467405823 56-57 23.83924357704668 27.428368381949152 22.176157801892003 26.55623023911216 58-59 23.422650423184624 27.19585624171713 25.581435660336627 23.800057674761618 60-61 25.910100601782577 29.53336437986136 24.017877757053828 20.538657261302237 62-63 19.04004866657447 30.195694631429106 26.878865854480168 23.88539084751626 64-65 16.677299449236312 31.403814955694987 27.854173127444838 24.064712467623867 66-67 23.80881271146138 28.9354225957282 24.667365118816324 22.588399573994096 68-69 26.68873167569953 26.815369066827426 23.6847383030826 22.811160954390445 70-71 21.226694282419942 31.054403191574043 23.764167456757782 23.954735069248233 72-73 24.97269163536516 28.76386500446864 23.302113265481438 22.961330094684758 74-75 26.840822438745654 26.435395998147836 23.53388569795967 23.18989586514684 76-77 22.253929487717507 28.230525742976493 29.595842696762038 19.919702072543966 78-79 24.581294161266413 27.865062200576123 27.855014915293502 19.698628722863955 80-81 20.024312636069 29.35433598329245 28.461388420959306 22.15996295967925 82-83 18.946404486014305 31.104958029318595 22.533269394521252 27.41536809014584 84-85 20.029165972191848 32.15857746339576 21.5531884964236 26.25906806798879 86-87 22.910336397607164 26.78787945657181 22.75937536572881 27.542408780092213 88-89 22.587641880242202 26.571951131101912 25.851636055311623 24.98877093334426 90-91 24.73536827398057 28.877669909033276 24.426458877815698 21.96050293917046 92-93 18.79734268230836 29.283967235504566 26.84325900099577 25.075431081191308 94-95 16.65394570981954 30.322558019581475 27.977068390149444 25.04642788044954 96-97 23.247526827417797 27.976818180206664 25.203571657893253 23.57208333448229 98-99 25.508245425968383 26.32266682839574 24.09435001059378 24.074737735042095 100-101 20.79700821629941 29.48132517807627 24.35483165900093 25.366834946623396 102-103 23.88982751540203 27.900201711734518 23.934083613313526 24.275887159549928 104-105 25.353323880650496 25.68943093720555 24.296689290367596 24.66055589177636 106-107 21.220862916094966 27.440883048388393 29.493809871124967 21.844444164391675 108-109 23.110264239850114 27.592782881892575 27.930905597791906 21.36604728046541 110-111 19.181078119251275 28.918942128081316 28.505762611448958 23.394217141218448 112-113 18.325172399802895 30.31463481020561 23.109156145949083 28.251036644042415 114-115 19.059190271663603 31.90058170863519 22.291360082687078 26.748867937014126 116-117 21.449363479707273 27.191238040632708 23.19688024723639 28.16251823242363 118-119 21.186707177225813 27.164891374167034 25.706393023356622 25.942008425250528 120-121 22.881933716289073 29.135244118518706 24.74498079966707 23.237841365525156 122-123 18.18813162398886 29.596253045483824 26.335827495130182 25.87978783539714 124-125 16.617232656540377 30.551478726831995 27.276977773996368 25.55431084263126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7512.0 1 4702.0 2 3006.0 3 3961.5 4 3412.5 5 2618.5 6 1996.5 7 1697.0 8 1599.0 9 1508.5 10 1304.5 11 1090.5 12 1029.5 13 1032.0 14 1099.5 15 1197.0 16 1257.0 17 1356.0 18 1458.5 19 1572.5 20 1766.5 21 2120.5 22 2575.5 23 3099.5 24 3683.5 25 4438.0 26 5587.0 27 6703.5 28 7504.5 29 8493.5 30 9902.0 31 11312.0 32 12432.5 33 13737.0 34 15686.0 35 17030.0 36 17949.5 37 19414.0 38 20059.5 39 19817.5 40 19455.0 41 20448.0 42 21628.5 43 22206.0 44 23500.0 45 24854.0 46 27075.0 47 33675.0 48 45804.5 49 81742.5 50 198282.0 51 212908.0 52 95851.5 53 41983.0 54 27915.0 55 21489.0 56 17539.0 57 14641.5 58 13072.5 59 11865.5 60 10632.0 61 9440.0 62 8022.0 63 5997.0 64 4158.5 65 3255.0 66 2431.0 67 1861.0 68 1477.0 69 1140.0 70 1005.0 71 887.0 72 859.0 73 883.0 74 648.5 75 470.5 76 356.0 77 248.0 78 191.5 79 111.5 80 73.5 81 63.5 82 58.5 83 56.0 84 43.0 85 33.5 86 25.0 87 18.5 88 17.5 89 14.5 90 16.5 91 15.5 92 12.0 93 9.0 94 6.0 95 7.0 96 5.5 97 4.5 98 4.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48687172558167635 2 0.4740035607153758 3 0.4593307141909965 4 0.46082153816940935 5 0.46294007750715394 6 0.46851105132122317 7 0.4526612384980968 8 0.48043764314852605 9 0.4920503772961632 10-11 0.4960912949218612 12-13 0.49565974061232065 14-15 0.4724735045270047 16-17 0.4749059015444152 18-19 0.46086077037936757 20-21 0.4699626430896778 22-23 0.48687172558167635 24-25 0.4751020625942064 26-27 0.48404700646468357 28-29 0.479927624419069 30-31 0.4920503772961632 32-33 0.4907164821575832 34-35 0.48577322370284587 36-37 0.4430493470583297 38-39 0.40468024571917743 40-41 0.3993054329548994 42-43 0.40624953411750675 44-45 0.4400284668915457 46-47 0.4474041223636936 48-49 0.44548174407574015 50-51 0.4309265941812355 52-53 0.4253556203671664 54-55 0.427474159704911 56-57 0.41005505848345536 58-59 0.41405674389919517 60-61 0.42370786754892065 62-63 0.4252379237372917 64-65 0.43849841070317463 66-67 0.42892575147336565 68-69 0.4304165754517785 70-71 0.44112696877037627 72-73 0.4410485043504598 74-75 0.4453248152359072 76-77 0.4365368002052629 78-79 0.42869035821361623 80-81 0.4374783732442605 82-83 0.4348105829671007 84-85 0.4330059013090219 86-87 0.4388122683828405 88-89 0.4264541222459969 90-91 0.43018118219202917 92-93 0.43857687512309107 94-95 0.43622294252559707 96-97 0.4074265004162537 98-99 0.3805524365948639 100-101 0.3807485976446551 102-103 0.35948473984729257 104-105 0.36325103200328296 106-107 0.381415545213945 108-109 0.3886735040562182 110-111 0.40487640676896863 112-113 0.4003647026237718 114-115 0.40009007715406414 116-117 0.37247060134346777 118-119 0.443951687887369 120-121 0.44999344822093695 122-123 0.40530796107850914 124-125 0.3996977550544818 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1274463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.872542331889946 #Duplication Level Percentage of deduplicated Percentage of total 1 87.08470468798099 42.560509163234855 2 8.703852524102926 8.507588018694948 3 2.082128855537207 3.0527679189807517 4 0.7710662712891171 1.5073587593707964 5 0.3913773225000352 0.9563802380812358 6 0.21304663071782362 0.6247278287054017 7 0.1356910797869861 0.4642097628664534 8 0.0975904915253542 0.3815596342610257 9 0.074575675034589 0.3280232551551498 >10 0.38621307651359976 3.418525517321813 >50 0.03085297403647426 1.0279401331896822 >100 0.020457594459368362 1.9086144044167814 >500 0.0027601515531581887 0.926494883197434 >1k 0.0017859804167494162 2.4293114807373626 >5k 0.0021107041288856735 7.145817935205698 >10k+ 0.0017859804167494162 24.760171066580554 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 78994 6.1982183868813765 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 56647 4.444773995008093 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 37999 2.9815694924058214 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 20911 1.640769484873237 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 20526 1.6105606832053971 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 20169 1.5825488852952183 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 19579 1.536254877544503 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16784 1.3169468238779785 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15583 1.2227110555583018 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12998 1.019880530074235 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 11846 0.9294895183304656 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8775 0.688525284766996 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8645 0.6783249101778553 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8065 0.6328155466263046 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7778 0.6102962581102785 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7441 0.5838537485984293 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7435 0.5833829620789305 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6655 0.5221807145440864 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 6598 0.5177082426088478 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 6528 0.512215733214695 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 6071 0.4763574933128698 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5768 0.45258277407818037 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 5241 0.41123202478220244 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5054 0.39655917825782305 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4993 0.39177284864291867 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4768 0.37411835416171363 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3817 0.2994986908211537 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3516 0.2758809004262972 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3116 0.24449513245971047 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 2731 0.21428633079187076 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2222 0.1743479410543892 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 1671 0.13111404568041599 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1438 0.11283183583987923 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9616104979116694E-4 0.0 12-13 0.0 0.0 0.0 0.001020037458914068 0.0 14-15 0.0 0.0 0.0 0.002118539337744603 0.0 16-17 0.0 0.0 0.0 0.0032562734265333716 0.0 18-19 0.0 0.0 0.0 0.00474709740494624 0.0 20-21 0.0 0.0 0.0 0.0068656367426908425 0.0 22-23 0.0 0.0 0.0 0.010671161108639481 0.0 24-25 0.0 0.0 0.0 0.015025936414003389 0.0 26-27 0.0 0.0 0.0 0.019498408349241995 0.0 28-29 0.0 0.0 0.0 0.028207958959969807 0.0 30-31 0.0 0.0 0.0 0.042096161285184425 0.0 32-33 0.0 0.0 0.0 0.06751863333811967 0.0 34-35 0.0 0.0 0.0 0.10867322158430649 0.0 36-37 0.0 0.0 0.0 0.1607343641988822 0.0 38-39 0.0 0.0 0.0 0.24147425229292652 0.0 40-41 0.0 0.0 0.0 0.3743145152115047 0.0 42-43 0.0 0.0 0.0 0.4731796843062529 0.0 44-45 0.0 0.0 0.0 0.5724371755005834 0.0 46-47 0.0 0.0 0.0 0.7039827754905399 0.0 48-49 0.0 0.0 0.0 0.8177561843694168 0.0 50-51 0.0 0.0 0.0 0.9371005670623628 0.0 52-53 0.0 0.0 0.0 1.0952848376139597 0.0 54-55 0.0 0.0 0.0 1.2520175163971021 0.0 56-57 0.0 0.0 0.0 1.4329172365145164 0.0 58-59 0.0 0.0 0.0 1.6414364324425268 0.0 60-61 0.0 0.0 0.0 1.8281032874238012 0.0 62-63 0.0 0.0 0.0 2.005236715385225 0.0 64-65 0.0 0.0 0.0 2.217404506839351 0.0 66-67 0.0 0.0 0.0 2.4514638714501715 0.0 68-69 0.0 0.0 0.0 2.7476670566348336 0.0 70-71 0.0 0.0 0.0 3.2169235199452633 0.0 72-73 0.0 0.0 0.0 3.5687972110606587 0.0 74-75 0.0 0.0 0.0 3.8945814825538285 0.0 76-77 0.0 0.0 0.0 4.28184262705155 0.0 78-79 0.0 0.0 0.0 4.643995157176002 0.0 80-81 0.0 0.0 0.0 5.033531769851303 0.0 82-83 0.0 0.0 0.0 5.539313420632847 0.0 84-85 0.0 0.0 0.0 6.0087268127831095 0.0 86-87 0.0 0.0 0.0 6.477434025154124 0.0 88-89 0.0 0.0 0.0 7.025743391530394 0.0 90-91 0.0 0.0 0.0 7.518735341865554 0.0 92-93 0.0 0.0 0.0 8.008157161094516 0.0 94-95 0.0 7.846441991646678E-5 0.0 8.565333006921346 0.0 96-97 0.0 7.846441991646678E-5 0.0 9.122744246007926 0.0 98-99 0.0 7.846441991646678E-5 0.0 9.778157545570174 0.0 100-101 0.0 7.846441991646678E-5 0.0 10.747938543527745 0.0 102-103 0.0 7.846441991646678E-5 0.0 11.521362330644358 0.0 104-105 0.0 7.846441991646678E-5 0.0 12.198549506733425 0.0 106-107 0.0 7.846441991646678E-5 0.0 12.949493237543969 0.0 108-109 0.0 7.846441991646678E-5 0.0 13.593293802958579 0.0 110-111 0.0 7.846441991646678E-5 0.0 14.267970117610318 0.0 112-113 0.0 7.846441991646678E-5 0.0 15.139513661832474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 150 0.0 59.521107 2 AGTACTC 1300 0.0 43.499622 5 AAGTACT 1320 0.0 41.03511 4 ATAACGC 255 0.0 37.34805 3 GAACAAA 1200 0.0 28.768534 1 TATCACG 170 4.4115586E-7 28.009932 2 GTGCGTT 85 0.007198176 28.009932 9 AAAGTAC 1945 0.0 27.542984 3 TCACGCA 175 5.681595E-7 27.210722 4 TTACGAA 55 7.7538326E-4 27.045454 78-79 CATTCCG 135 8.226978E-5 26.453827 9 CATGGGG 5480 0.0 25.634188 4 AAAAAGT 2095 0.0 25.569925 1 AAAAGTA 2160 0.0 25.351583 2 ATGGGGG 1510 0.0 25.229477 5 GTACAAG 745 0.0 24.76717 1 ATCACGC 195 1.4599864E-6 24.419878 3 GTACAAA 1275 0.0 23.808443 1 ATGGGGT 930 0.0 22.402182 5 ATGGGAT 2535 0.0 21.60305 5 >>END_MODULE