##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364149_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1274463 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04886921001238 33.0 31.0 34.0 30.0 34.0 2 31.823582167548214 33.0 31.0 34.0 30.0 34.0 3 32.08041504539559 34.0 31.0 34.0 30.0 34.0 4 35.53085809474265 37.0 35.0 37.0 33.0 37.0 5 35.68857628663994 37.0 35.0 37.0 33.0 37.0 6 35.85088072388135 37.0 35.0 37.0 35.0 37.0 7 35.816988017698435 37.0 35.0 37.0 35.0 37.0 8 35.88184278398039 37.0 35.0 37.0 35.0 37.0 9 37.52971408350027 39.0 37.0 39.0 35.0 39.0 10-11 37.4833733894197 39.0 37.0 39.0 34.0 39.0 12-13 37.46791354476356 39.0 37.0 39.0 34.0 39.0 14-15 38.719897321460095 40.0 38.0 41.0 34.0 41.0 16-17 38.6855161742632 40.0 38.0 41.0 34.0 41.0 18-19 38.747620370304986 40.0 38.0 41.0 34.0 41.0 20-21 38.781394595213825 40.0 38.0 41.0 34.0 41.0 22-23 38.72398688702614 40.0 38.0 41.0 34.5 41.0 24-25 38.69010124264102 40.0 38.0 41.0 34.0 41.0 26-27 38.46495072826751 40.0 38.0 41.0 34.0 41.0 28-29 38.2750240689608 40.0 38.0 41.0 34.0 41.0 30-31 38.074953137125206 40.0 37.0 41.0 34.0 41.0 32-33 37.65886808797117 40.0 36.5 41.0 33.0 41.0 34-35 37.36853796461725 40.0 36.0 41.0 33.0 41.0 36-37 37.23038056028304 40.0 36.0 41.0 32.5 41.0 38-39 37.11786375908912 40.0 36.0 41.0 32.0 41.0 40-41 36.828878515892576 40.0 36.0 41.0 31.0 41.0 42-43 36.61650318604777 40.0 35.0 41.0 30.5 41.0 44-45 36.32303958608449 40.0 35.0 41.0 30.0 41.0 46-47 35.916875185862594 39.0 35.0 41.0 29.5 41.0 48-49 35.5114585515625 39.0 35.0 41.0 27.0 41.0 50-51 35.34967041020414 39.0 35.0 41.0 26.5 41.0 52-53 35.270438215938796 39.0 35.0 41.0 26.0 41.0 54-55 35.08640580385621 39.0 35.0 41.0 25.5 41.0 56-57 34.77824974126358 39.0 35.0 41.0 23.5 41.0 58-59 34.6019166503853 38.0 34.5 40.0 23.0 41.0 60-61 34.4114234779668 38.0 34.0 40.0 23.0 41.0 62-63 34.0375730797991 38.0 34.0 40.0 22.0 41.0 64-65 33.774182538057204 37.0 34.0 40.0 22.0 41.0 66-67 33.28878986679096 37.0 33.5 39.0 21.5 41.0 68-69 32.967614203001574 36.0 33.0 39.0 20.5 41.0 70-71 32.43686713541311 35.0 33.0 39.0 18.5 40.0 72-73 32.09187398928019 35.0 32.5 37.5 18.0 39.0 74-75 31.76143560071968 35.0 32.0 37.0 15.0 39.0 76-77 31.2617859443546 35.0 32.0 36.5 12.0 38.5 78-79 30.85238488681115 35.0 31.5 36.0 7.5 37.0 80-81 30.631606409915392 35.0 31.5 35.5 7.0 37.0 82-83 30.430955626016605 35.0 31.5 35.0 4.0 36.5 84-85 30.182835045034654 35.0 31.0 35.0 2.0 36.0 86-87 29.89265557336698 35.0 31.0 35.0 2.0 36.0 88-89 29.665227236883297 34.0 31.0 35.0 2.0 35.5 90-91 29.492732625427337 34.0 31.0 35.0 2.0 35.0 92-93 29.267963448134626 34.0 30.0 35.0 2.0 35.0 94-95 29.20182931948593 34.0 30.0 35.0 2.0 35.0 96-97 29.10753352588502 34.0 30.0 35.0 2.0 35.0 98-99 28.97759526953705 34.0 30.0 35.0 2.0 35.0 100-101 28.673544857716543 34.0 29.0 35.0 2.0 35.0 102-103 27.204578712759805 32.5 26.0 34.5 2.0 35.0 104-105 28.09860937508582 34.0 29.0 35.0 2.0 35.0 106-107 28.179250398010772 34.0 29.0 35.0 2.0 35.0 108-109 28.10987137327643 34.0 29.0 35.0 2.0 35.0 110-111 28.10131482828454 34.0 29.0 35.0 2.0 35.0 112-113 28.014375858694997 34.0 29.0 35.0 2.0 35.0 114-115 27.88045435606997 34.0 29.0 35.0 2.0 35.0 116-117 27.59218549302726 34.0 27.5 35.0 2.0 35.0 118-119 27.355380658363565 34.0 27.0 35.0 2.0 35.0 120-121 27.135107492332065 34.0 27.0 35.0 2.0 35.0 122-123 26.76320811196559 34.0 25.0 35.0 2.0 35.0 124-125 25.65225353737221 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1599352736583839 1101 2 0.5025075158710273 1101 3 0.3881546096181836 1101 4 0.5168904923410267 1101 5 0.20839090846633468 1101 6 0.24890516749063352 1101 7 0.113788172455763 1101 8 0.11449356146682277 1101 9 0.2124196147040749 1101 10-11 0.2190459986382436 1101 12-13 0.1687806920742574 1101 14-15 0.2806205049159729 1101 16-17 0.3002791339787052 1101 18-19 0.19448661794587707 1101 20-21 0.11362061311299243 1101 22-23 0.06992990510565988 1101 24-25 0.1100645007982024 1101 26-27 0.13755188983526523 1101 28-29 0.17454973461991585 1101 30-31 0.14416405446082337 1101 32-33 0.11434796131835157 1101 34-35 0.11765910741434737 1101 36-37 0.20031573371863942 1101 38-39 0.11874899453211185 1101 40-41 0.26992865608450245 1101 42-43 0.3408164437346173 1101 44-45 0.33520085173949354 1101 46-47 0.3421036722084523 1101 48-49 0.23997770000784868 1101 50-51 0.26034508984183447 1101 52-53 0.18377366720315536 1101 54-55 0.19244292060018608 1101 56-57 0.2920289213548344 1101 58-59 0.3460948709946976 1101 60-61 0.35388575755219165 1101 62-63 0.27269315655159687 1101 64-65 0.27630699005831616 1101 66-67 0.24217929478169964 1101 68-69 0.262301676090793 1101 70-71 0.32529796570360503 1101 72-73 0.22762434608382165 1101 74-75 0.13209539069733012 1101 76-77 0.1384026960812541 1101 78-79 0.1800155040972733 1101 80-81 0.19206314824215198 1101 82-83 0.2524299635802585 1101 84-85 0.2005512835146419 1101 86-87 0.4536115553683615 1101 88-89 0.29842742986530624 1101 90-91 0.3704817981955273 1101 92-93 0.36019847752237055 1101 94-95 0.4038696746442554 1101 96-97 0.42345918841142804 1101 98-99 0.2645992936180406 1101 100-101 0.36657668234653684 1101 102-103 0.15283582832104514 1101 104-105 0.11470962204099067 1101 106-107 0.1394825885049329 1101 108-109 0.17060825274996816 1101 110-111 0.15131875101157988 1101 112-113 0.16869597744577547 1101 114-115 0.28412578895324003 1101 116-117 0.4113840193419165 1101 118-119 0.2803920887375462 1101 120-121 0.1498447033208059 1101 122-123 0.21131908355236462 1101 124-125 0.3173412129407609 1102 1 0.2888782710898994 1102 2 0.5783813489163272 1102 3 0.32998879771461276 1102 4 0.4917640985076517 1102 5 0.30664618339568506 1102 6 0.1640108061261074 1102 7 0.2721189615734332 1102 8 0.24878165257681673 1102 9 0.3078949255418735 1102 10-11 0.2795489410586782 1102 12-13 0.19521093808356937 1102 14-15 0.4380182904680012 1102 16-17 0.4843451080585126 1102 18-19 0.3452461013347161 1102 20-21 0.24820532709180299 1102 22-23 0.3074927519009307 1102 24-25 0.4012901778630038 1102 26-27 0.35809491415609784 1102 28-29 0.5253760211761005 1102 30-31 0.44801020830697524 1102 32-33 0.5714474434187409 1102 34-35 0.5158767092786718 1102 36-37 0.6113340548724153 1102 38-39 0.7355377368679541 1102 40-41 0.6985678881701958 1102 42-43 0.7756627684400286 1102 44-45 0.9195512644626262 1102 46-47 0.7956156701693544 1102 48-49 0.7613425293306619 1102 50-51 0.7627987407679768 1102 52-53 0.7342849845280028 1102 54-55 0.6135646256074878 1102 56-57 0.6493027639234299 1102 58-59 0.734308523489382 1102 60-61 0.7494436970456064 1102 62-63 0.8729494454325817 1102 64-65 0.9044578727844481 1102 66-67 0.8154167135185233 1102 68-69 0.8121404801880558 1102 70-71 0.6169313241523255 1102 72-73 0.6476542324484242 1102 74-75 0.6113527971658925 1102 76-77 0.6059474205917112 1102 78-79 0.6802140929678941 1102 80-81 0.5794443520436268 1102 82-83 0.638068262942479 1102 84-85 0.5367118239900783 1102 86-87 0.5827079059827511 1102 88-89 0.514020017374655 1102 90-91 0.5832056308206788 1102 92-93 0.6744580827534641 1102 94-95 0.6896401798554628 1102 96-97 0.657725992138964 1102 98-99 0.7778018122515036 1102 100-101 0.6557443456921881 1102 102-103 0.4976148176745525 1102 104-105 0.5965619884416604 1102 106-107 0.7056499353408157 1102 108-109 0.6044469280025453 1102 110-111 0.5317006074393902 1102 112-113 0.6662617932707029 1102 114-115 0.5887626570190818 1102 116-117 0.6481319235150877 1102 118-119 0.6641893096137501 1102 120-121 0.6052368533184236 1102 122-123 0.5809242026076546 1102 124-125 0.553793614633669 1103 1 0.23400453980550395 1103 2 0.5344788564244887 1103 3 0.30068831763043846 1103 4 0.4130829050929137 1103 5 0.11370139307628335 1103 6 0.10502813519202903 1103 7 0.1316343286514936 1103 8 0.14568110891984531 1103 9 0.2792940201776446 1103 10-11 0.26421255330849647 1103 12-13 0.2151832071937676 1103 14-15 0.25329137944799385 1103 16-17 0.35847454445283944 1103 18-19 0.2492313206608472 1103 20-21 0.21784055080226494 1103 22-23 0.22768067526283886 1103 24-25 0.1775669770825914 1103 26-27 0.12582144702761866 1103 28-29 0.2506851164695121 1103 30-31 0.20433967429553235 1103 32-33 0.2606366437168717 1103 34-35 0.45952162235931837 1103 36-37 0.4676584514789397 1103 38-39 0.5261086862946982 1103 40-41 0.5816901342626579 1103 42-43 0.4377354332360426 1103 44-45 0.48327120439729754 1103 46-47 0.46250972830556947 1103 48-49 0.43164129404556917 1103 50-51 0.5041033804856312 1103 52-53 0.5218429398955209 1103 54-55 0.5909618952657283 1103 56-57 0.4907278357168465 1103 58-59 0.34799599197700815 1103 60-61 0.2983117177396508 1103 62-63 0.2805679045596605 1103 64-65 0.2246710060461723 1103 66-67 0.26701822720859525 1103 68-69 0.3499831430111229 1103 70-71 0.3381760081517058 1103 72-73 0.3533838672336955 1103 74-75 0.33094130035549796 1103 76-77 0.30799545093127634 1103 78-79 0.2892199186469142 1103 80-81 0.2148782363411499 1103 82-83 0.24063679550709338 1103 84-85 0.18974262939157427 1103 86-87 0.262262951250964 1103 88-89 0.32072626175722974 1103 90-91 0.18990639479024907 1103 92-93 0.23376752331290973 1103 94-95 0.20225403543561882 1103 96-97 0.19820076031749068 1103 98-99 0.22974831794017447 1103 100-101 0.36836135250981883 1103 102-103 0.1364416256628047 1103 104-105 0.29883772994153546 1103 106-107 0.21831242958637986 1103 108-109 0.2156319029022562 1103 110-111 0.20679788477021077 1103 112-113 0.30854413866825325 1103 114-115 0.25238216838545924 1103 116-117 0.2677412522740994 1103 118-119 0.17804900008002278 1103 120-121 0.22606735510504095 1103 122-123 0.31576483219750173 1103 124-125 0.2030197803175149 1104 1 0.13026872062587813 1104 2 0.43810133749865443 1104 3 0.0011069636467908595 1104 4 0.29548458835358105 1104 5 0.07871326917441479 1104 6 0.09919881478683124 1104 7 0.10626475966166993 1104 8 0.13488063821422713 1104 9 0.17697601189149026 1104 10-11 0.21668213097492384 1104 12-13 0.13617461735841374 1104 14-15 0.14138789579254052 1104 16-17 0.14282168442810672 1104 18-19 0.13406096101572018 1104 20-21 0.21912577238096276 1104 22-23 0.0731165117956678 1104 24-25 0.11761396630191712 1104 26-27 0.17561198844754244 1104 28-29 0.22322963828032272 1104 30-31 0.23274673162617887 1104 32-33 0.17328889095663413 1104 34-35 0.23935394703774193 1104 36-37 0.2747418119266598 1104 38-39 0.37269390136595604 1104 40-41 0.3983722561255263 1104 42-43 0.459857648589292 1104 44-45 0.45832678887485656 1104 46-47 0.3665089636800758 1104 48-49 0.3085399784558831 1104 50-51 0.34355439564798473 1104 52-53 0.3689383120132561 1104 54-55 0.3659069886476516 1104 56-57 0.32337490411015324 1104 58-59 0.34302633296744744 1104 60-61 0.27576421334711654 1104 62-63 0.2625844280624108 1104 64-65 0.2512146276595857 1104 66-67 0.15856437045928118 1104 68-69 0.30765556334907984 1104 70-71 0.2791318713650881 1104 72-73 0.32285212714128875 1104 74-75 0.3486948469163096 1104 76-77 0.23143040895822153 1104 78-79 0.2952025708670405 1104 80-81 0.19309264600688536 1104 82-83 0.19890356303925216 1104 84-85 0.16717194220474596 1104 86-87 0.1424821981219644 1104 88-89 0.07717853502678906 1104 90-91 0.15191494666548166 1104 92-93 0.1448194798865181 1104 94-95 0.10200607443693954 1104 96-97 0.08443777252980667 1104 98-99 0.06537186732088429 1104 100-101 0.0165392662210202 1104 102-103 0.008858438747118669 1104 104-105 0.12604185783463961 1104 106-107 0.25336535685476846 1104 108-109 0.19763392021207338 1104 110-111 0.2472815510681201 1104 112-113 0.35376292459480396 1104 114-115 0.22022112524106063 1104 116-117 0.25865915535646167 1104 118-119 0.06968719664842737 1104 120-121 0.25264107367947375 1104 122-123 0.21060388124199036 1104 124-125 0.3670151383357769 1105 1 0.09364949469925676 1105 2 0.3867935080012721 1105 3 0.06580471669821009 1105 4 0.34312306642623724 1105 5 0.003112066359108212 1105 6 0.013197247084768549 1105 7 0.034027561423833674 1105 8 0.030511283238951137 1105 9 0.1038413451920519 1105 10-11 0.03076727850005767 1105 12-13 0.05035726830436005 1105 14-15 0.1832708042895419 1105 16-17 0.21500903848023967 1105 18-19 0.19083963371284796 1105 20-21 0.10009990529697177 1105 22-23 0.2625783584612975 1105 24-25 0.11281677068602392 1105 26-27 0.13921330597275983 1105 28-29 0.29895074026016744 1105 30-31 0.25654698890411254 1105 32-33 0.17746446812498817 1105 34-35 0.29459141020084445 1105 36-37 0.3710842046114351 1105 38-39 0.24557811810213792 1105 40-41 0.2610213448645595 1105 42-43 0.1810471630113284 1105 44-45 0.19642672851560405 1105 46-47 0.10774922357994399 1105 48-49 0.30286730044386445 1105 50-51 0.2436666474853979 1105 52-53 0.23519652947592817 1105 54-55 0.17771268645259397 1105 56-57 0.173378591214302 1105 58-59 0.17550193662832925 1105 60-61 0.1982002420901381 1105 62-63 0.2764203546589954 1105 64-65 0.2623571929554629 1105 66-67 0.2040551786451985 1105 68-69 0.17038399310701635 1105 70-71 0.07267288425356355 1105 72-73 -0.011286360490522895 1105 74-75 -0.04152039803031471 1105 76-77 -0.028062245510962924 1105 78-79 0.05216942243515632 1105 80-81 -0.015820996551585864 1105 82-83 -0.008524449666236222 1105 84-85 -0.015864101286698684 1105 86-87 0.10061089717505922 1105 88-89 0.03166382894361419 1105 90-91 0.02740376108397058 1105 92-93 -0.06193829922044003 1105 94-95 0.00261130575492885 1105 96-97 0.036674479153759876 1105 98-99 -0.1412180802676133 1105 100-101 -0.10889847497704253 1105 102-103 -0.16202340731764764 1105 104-105 -0.03471042065916308 1105 106-107 0.03901938085098777 1105 108-109 0.01674622940443271 1105 110-111 0.21693012907495657 1105 112-113 0.16219096188098803 1105 114-115 0.1902285813850071 1105 116-117 0.17522640677053758 1105 118-119 0.1424676282307935 1105 120-121 0.07121725061677608 1105 122-123 0.12224761958323072 1105 124-125 0.13148401781117158 1106 1 0.15922556613647032 1106 2 0.48402292288188065 1106 3 0.37066076942107173 1106 4 0.43477124728865135 1106 5 0.2588268014945072 1106 6 0.184079483772976 1106 7 0.08303336126088823 1106 8 0.015472308441495386 1106 9 0.2709433357434534 1106 10-11 0.23050336843652275 1106 12-13 0.24163963190601123 1106 14-15 0.3313246188184351 1106 16-17 0.3254276377537124 1106 18-19 0.16035244202245025 1106 20-21 0.1498893687918823 1106 22-23 0.13334968032402372 1106 24-25 0.11420596206988876 1106 26-27 0.12407686057108691 1106 28-29 0.23675992373883048 1106 30-31 0.2838281371481628 1106 32-33 0.31641896481367837 1106 34-35 0.22533941978388583 1106 36-37 0.2238557308263296 1106 38-39 0.2019233872151105 1106 40-41 0.15557993074759224 1106 42-43 0.25743440498311543 1106 44-45 0.3961240990948056 1106 46-47 0.43870575303489545 1106 48-49 0.3680752691026896 1106 50-51 0.39502034612510784 1106 52-53 0.4010996988166511 1106 54-55 0.5515648860616409 1106 56-57 0.5798033156749867 1106 58-59 0.7642922793753044 1106 60-61 0.6294702907851075 1106 62-63 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-1.8215368880402458 2216 124-125 -2.0046477278761508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62.0 3 24.0 4 80.0 5 250.0 6 737.0 7 1289.0 8 2265.0 9 4511.0 10 9453.0 11 11740.0 12 9424.0 13 10997.0 14 15493.0 15 15102.0 16 8790.0 17 7862.0 18 8182.0 19 8093.0 20 8291.0 21 7913.0 22 8167.0 23 8888.0 24 10143.0 25 11432.0 26 13207.0 27 15805.0 28 19345.0 29 23735.0 30 29109.0 31 36468.0 32 46999.0 33 61339.0 34 87326.0 35 136776.0 36 216775.0 37 304572.0 38 113158.0 39 661.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.10437498772174 17.156631239809048 10.64833113961849 23.090662632850716 2 12.167986573126322 23.780835633001924 36.120973683735606 27.930204110136142 3 23.11235294717672 30.432647001821167 28.25587681655795 18.19912323444416 4 13.633114496066186 19.466630259175826 32.54986610046741 34.350389144290574 5 10.713296502134623 39.19792100672989 35.25406386846852 14.834718622666959 6 31.15798228991341 28.194347994962392 20.96452097629108 19.68314873883311 7 21.811382519539602 32.85681891118063 25.026932912136328 20.304865657143438 8 28.243032555672464 30.97092657848835 24.449199388291383 16.336841477547797 9 29.78423069167171 14.100213187828913 22.092442071680384 34.02311404881899 10-11 23.151437115082977 27.65658948121679 26.41022140305368 22.781752000646545 12-13 27.78796345084936 25.43609960345843 26.41605595132116 20.35988099437105 14-15 28.521445873131828 22.258190061368083 27.012352866864763 22.208011198635326 16-17 21.953401221228855 26.287021736367105 33.64601625480402 18.113560787600022 18-19 24.3573175525692 23.855694516043226 34.4253226653108 17.36166526607677 20-21 20.44325370234656 24.465656881099633 35.28331954973942 19.80776986681439 22-23 19.02950497582119 26.691555580664172 29.137605407140104 25.141334036374534 24-25 20.552617063029686 28.13502628165745 27.781151747834187 23.531204907478678 26-27 22.97602990435972 22.916828499532745 29.41509482817469 24.692046767932847 28-29 22.09816542051895 22.15720998916405 33.31451492042518 22.43010966989182 30-31 24.92488993403496 24.327226447531235 31.444380888264313 19.30350273016949 32-33 18.26224064566802 24.862393023571496 33.796116482000656 23.07924984875983 34-35 16.32432640257112 26.590218782342053 34.958802256322855 22.126652558763965 36-37 23.489814925972745 24.440646766520487 30.56589324287955 21.503645064627218 38-39 25.428553045478765 22.679002842765932 30.291503166431667 21.60094094532364 40-41 21.061772683867634 25.902674302824014 30.4365054144373 22.599047598871053 42-43 24.77969152497954 24.56693525037604 29.482495764882938 21.170877459761485 44-45 26.88626503868688 21.798161264783676 29.446559060561196 21.86901463596825 46-47 22.03202446834471 24.43966596127153 35.27344458018789 18.25486499019587 48-49 24.783418584925574 24.028355472069414 32.800795315360276 18.38743062764474 50-51 20.362929327881627 25.36570304512567 33.04764438041748 21.223723246575226 52-53 19.312016119730426 27.326097344528637 27.200907362551913 26.16097917318902 54-55 20.738970060331294 28.38795633925818 25.94245576372166 24.930617836688864 56-57 23.304913520439587 23.575262679261773 27.16551990916959 25.95430389112905 58-59 22.54694722404652 22.75770265594215 30.87009979889569 23.825250321115636 60-61 25.069617556570883 24.969928511067014 29.12465877785389 20.83579515450821 62-63 18.892270705387286 25.463077390242006 31.422999333837076 24.221652570533628 64-65 16.79303361494214 26.831849963474813 32.97000383691013 23.405112584672917 66-67 23.450661180434427 25.11967785647759 28.789654937020533 22.64000602606745 68-69 25.285904730070623 23.321587209671836 28.593925441538907 22.79858261871863 70-71 21.150359013953327 26.191305671486738 28.85411345798191 23.804221856578025 72-73 24.702717929041487 24.932500982766857 28.019016636810957 22.3457644513807 74-75 26.360749586296343 22.205626997409887 28.274849877948594 23.15877353834517 76-77 21.77744665792573 24.566425231646583 34.14532238283889 19.510805727588796 78-79 24.158488712500873 24.264572608227937 32.012620217299364 19.564318461971826 80-81 19.849693557208017 25.516511660205122 32.35511741023474 22.27867737235212 82-83 18.87532239068533 27.269995284288363 26.80744752888079 27.047234796145514 84-85 20.262337941548715 28.04797000776013 25.77552271034938 25.914169340341775 86-87 22.575939827205655 23.345715018796152 27.118794347109333 26.95955080688886 88-89 21.859363512318524 22.69908973426455 30.449059721623932 24.992487031792997 90-91 24.110900041821537 24.782712405146324 28.771176566130208 22.335210986901934 92-93 18.561896265329008 25.204850984296918 30.677548112420684 25.555704637953397 94-95 16.629127877347223 26.464787621732977 32.22283642595155 24.68324807496825 96-97 22.813881245002705 24.714913777748485 28.49932401629266 23.971880960956156 98-99 24.123671702280838 23.274973566458534 28.214936569308847 24.386418161951788 100-101 20.6051097599538 25.545465031154297 28.58760905573563 25.261816153156268 102-103 23.570586172073433 24.462089058482245 28.00255959372923 23.96476517571509 104-105 25.18356500665511 21.875981001531933 28.305268841507825 24.63518515030513 106-107 20.939084850375412 24.200396696938842 33.45595231789119 21.40456613479456 108-109 23.009063809232693 24.23175005405168 31.17842076494799 21.58076537176764 110-111 19.20794200369147 25.237152040982885 31.796030410707782 23.758875544617858 112-113 18.32571480140155 26.63098708707884 26.877866758743004 28.16543135277661 114-115 19.309530920752337 27.675279214772353 25.89172703221297 27.12346283226234 116-117 21.42536435344973 23.439267360508772 27.017023177173073 28.118345108868425 118-119 20.712060210800587 23.02588311005423 29.864776838619044 26.39727984052614 120-121 22.519056563277758 24.739964772774982 28.48684982364807 24.25412884029919 122-123 17.958642898253462 25.352891258214903 29.6924954676047 26.99597037592693 124-125 16.201520826026197 26.613513867670402 31.097251418074933 26.08771388822847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1961.0 1 1574.0 2 3758.5 3 5010.0 4 2427.5 5 1222.5 6 1707.0 7 3288.5 8 4832.5 9 4973.0 10 3790.0 11 2492.5 12 2086.5 13 2044.5 14 2331.5 15 2754.0 16 2912.5 17 3176.5 18 3512.0 19 3578.0 20 3676.5 21 4004.0 22 4430.5 23 4744.0 24 5173.0 25 5738.0 26 6513.0 27 7348.5 28 7916.5 29 8701.5 30 9884.5 31 11039.5 32 12056.5 33 13239.5 34 14756.5 35 15690.0 36 16600.5 37 18045.0 38 18537.5 39 17928.0 40 17296.5 41 18172.0 42 19484.0 43 20126.0 44 21219.5 45 22816.5 46 25200.5 47 32856.5 48 45268.5 49 86116.5 50 225224.5 51 228808.5 52 81316.0 53 28940.0 54 21043.0 55 17653.0 56 14888.5 57 12724.5 58 11702.0 59 10757.0 60 9576.0 61 8439.0 62 7137.0 63 5311.5 64 3910.0 65 3096.5 66 2161.0 67 1670.5 68 1345.5 69 969.0 70 785.5 71 726.0 72 798.0 73 821.0 74 570.5 75 421.5 76 297.5 77 150.0 78 92.5 79 42.0 80 17.0 81 13.0 82 10.0 83 6.0 84 2.5 85 3.5 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1481408248022893 2 3.138576796658671E-4 3 4.7078651949880064E-4 4 0.0 5 0.0 6 0.003766292155990405 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005453277184194441 14-15 0.0017262172381622691 16-17 7.06179779248201E-4 18-19 0.0 20-21 7.846441991646678E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.5692883983293356E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 2.7462546970763376E-4 96-97 0.0018831460779952026 98-99 0.0031778090066169047 100-101 0.0 102-103 0.003295505636491605 104-105 0.02000842707869903 106-107 0.01494747199408692 108-109 0.017222940171664456 110-111 0.013731273485381685 112-113 0.01239737834680175 114-115 0.03832986912919402 116-117 0.03876142343873459 118-119 0.02142078663719543 120-121 0.012907397076258785 122-123 0.0020793071277863695 124-125 0.0018831460779952026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1274463.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.306365733703494 #Duplication Level Percentage of deduplicated Percentage of total 1 86.50995350516048 40.92471500120806 2 9.084667648928676 8.595252207387285 3 2.1464749288130505 3.04625784061966 4 0.7949131868704026 1.504178157785402 5 0.3864547210232216 0.9140884186120437 6 0.2362665432501476 0.6706146903377614 7 0.14828152972943537 0.4910262193853588 8 0.10254326748725646 0.3880759452224913 9 0.07791106084478158 0.3317120225121657 >10 0.4308819883403902 3.7806894314455413 >50 0.04241397932037193 1.405596787516349 >100 0.02921951942356204 2.888310865772561 >500 0.003840279643174338 1.2501243128830073 >1k 0.0021705928417941906 2.327583449297811 >5k 0.0023375615219322055 8.023164668313916 >10k+ 0.0016696868013801468 23.45860998170063 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 69553 5.457435798450014 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 61584 4.83215283613569 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 36708 2.8802719262936627 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 24377 1.9127271643037107 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22268 1.7472457026998824 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 20084 1.575879409602319 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18347 1.439586712207416 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 17381 1.363790082568109 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 14253 1.118353377069401 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12439 0.9760189193409303 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9810 0.7697359593805391 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9212 0.722814236270492 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8551 0.6709492547057074 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8078 0.6338355840852186 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7907 0.6204181682795028 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7719 0.6056668573352071 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7395 0.5802443852822718 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7018 0.5506632989737639 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6688 0.5247700404013298 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6378 0.5004460702272251 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6152 0.4827131113261036 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 5920 0.46450936590548336 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5719 0.4487380175022735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5029 0.3945975677599114 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3627 0.284590451037025 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3470 0.27227153711013974 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 3346 0.2625419490404978 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3160 0.24794756693603504 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2512 0.19710262283016455 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2324 0.1823513118858688 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2291 0.1797619860286254 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2029 0.15920430801051108 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1727 0.1355080531957381 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 1357 0.10647621782664543 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 1350 0.10592696688723016 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9616104979116694E-4 0.0 12-13 0.0 0.0 0.0 0.001020037458914068 0.0 14-15 0.0 0.0 0.0 0.002118539337744603 0.0 16-17 0.0 0.0 0.0 0.003295505636491605 0.0 18-19 0.0 0.0 0.0 0.0047863296149044735 0.0 20-21 0.0 0.0 0.0 0.006944101162607311 0.0 22-23 0.0 0.0 0.0 0.010671161108639481 0.0 24-25 0.0 0.0 0.0 0.015025936414003389 0.0 26-27 0.0 0.0 0.0 0.019655337189074928 0.0 28-29 0.0 0.0 0.0 0.028443352219719206 0.0 30-31 0.0 0.0 0.0 0.042292322334975596 0.0 32-33 0.0 0.0 0.0 0.06771479438791084 0.0 34-35 0.0 0.0 0.0 0.10926170473367999 0.0 36-37 0.0 0.0 0.0 0.16171516944783804 0.0 38-39 0.0 0.0 0.0 0.2424158253319241 0.0 40-41 0.0 0.0 0.0 0.3752168560405441 0.0 42-43 0.0 0.0 0.0 0.4738466318755429 0.0 44-45 0.0 0.0 0.0 0.5727902653902075 0.0 46-47 0.0 0.0 0.0 0.7045320264299553 0.0 48-49 0.0 0.0 0.0 0.818815454038289 0.0 50-51 0.0 0.0 0.0 0.9395721962897314 0.0 52-53 0.0 0.0 0.0 1.0988157365102007 0.0 54-55 0.0 0.0 0.0 1.2570784714817143 0.0 56-57 0.0 0.0 0.0 1.4401751953567894 0.0 58-59 0.0 0.0 0.0 1.649400571064048 0.0 60-61 0.0 0.0 0.0 1.8377936432834847 0.0 62-63 0.0 0.0 0.0 2.016810217322904 0.0 64-65 0.0 0.0 0.0 2.2316065668442318 0.0 66-67 0.0 0.0 0.0 2.46770600637288 0.0 68-69 0.0 0.0 0.0 2.7657923376355376 0.0 70-71 0.0 0.0 0.0 3.239050486361707 0.0 72-73 0.0 0.0 0.0 3.5932781100745963 0.0 74-75 0.0 0.0 0.0 3.9224363516241745 0.0 76-77 0.0 0.0 0.0 4.3126791440787215 0.0 78-79 0.0 0.0 0.0 4.678048715419749 0.0 80-81 0.0 0.0 0.0 5.07135162025104 0.0 82-83 0.0 0.0 0.0 5.583567353465734 0.0 84-85 0.0 0.0 0.0 6.061533367386891 0.0 86-87 0.0 0.0 0.0 6.533810710864105 0.0 88-89 0.0 7.846441991646678E-5 0.0 7.086396388125822 0.0 90-91 0.0 7.846441991646678E-5 0.0 7.584959312275052 0.0 92-93 0.0 7.846441991646678E-5 0.0 8.081835251396079 0.0 94-95 0.0 7.846441991646678E-5 0.0 8.645680572915808 0.0 96-97 0.0 7.846441991646678E-5 0.0 9.210859789574119 0.0 98-99 0.0 7.846441991646678E-5 0.0 9.875806516156217 0.0 100-101 0.0 7.846441991646678E-5 0.0 10.853473188315393 0.0 102-103 0.0 7.846441991646678E-5 0.0 11.63635193803194 0.0 104-105 0.0 7.846441991646678E-5 0.0 12.323621792080273 0.0 106-107 0.0 7.846441991646678E-5 0.0 13.084138182120627 0.0 108-109 0.0 7.846441991646678E-5 0.0 13.739159159583291 0.0 110-111 0.0 7.846441991646678E-5 0.0 14.429057571698825 0.0 112-113 0.0 7.846441991646678E-5 0.0 15.318255610402185 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 160 0.0 63.217037 3 TATAACG 165 0.0 61.301373 2 AGTACTC 1485 0.0 50.08282 5 TAACGCA 215 0.0 49.812603 4 GTACCGT 65 3.5177094E-5 45.775177 6 GTTATCA 580 0.0 45.205883 1 AAGTACT 1630 0.0 44.53254 4 AAAGTAC 1605 0.0 44.11407 3 CGCATGT 105 3.225814E-7 39.72638 1 GCAACGC 105 3.264795E-7 39.665592 4 AAAAAGT 1840 0.0 38.862762 1 AAAAGTA 1835 0.0 38.58479 2 TTATCAA 710 0.0 36.034237 2 GCGTCAG 80 0.00532313 29.794785 1 GATTACA 405 0.0 29.426949 1 ATAGTAC 240 4.3655746E-10 27.270098 3 TGTACCG 160 8.744153E-6 26.030544 5 TATCACG 115 0.0010050316 25.868866 2 GTCAACG 140 1.0562025E-4 25.49931 2 AACCGAT 60 0.0012880533 24.792456 98-99 >>END_MODULE