FastQCFastQC Report
Thu 26 May 2016
SRR1364148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523078
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA520023.414270313142203No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT377722.4799780444599686No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG252021.654675597704123No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC136860.8985751222196106No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT130270.855307476045219No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG122460.8040297345244302No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC115480.7582014840999607No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG104400.6854540607900581No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT87310.573247069421264No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG83330.5471157747666239No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC58470.3838936679539722No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56630.37181286841514355No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT55830.3665603468765224No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG52170.34253006083733073No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA49610.3257219919137431No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG47640.3127876576248885No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC45730.30024726245143063No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG43500.2856058586625242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG39720.2607876943925393No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC35980.23623215619948554No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA33790.22185337848751016No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC25590.16801503271664353No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG19380.12724233427309697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC11600.034.908425
ATAACGC1754.4201443E-1034.0250053
TATAACG2502.1827873E-1128.5744022
TAACGCT1701.3911993E-524.5180194
CATGGGT43350.024.0372714
AAGTACT17350.024.0234184
CATGGGG58800.022.5820714
ATGGGGG22250.021.6787515
ATGGGTG20700.020.7129215
GTATAGG5200.020.605181
ATGGGAG19550.020.1037185
GTGTAGC6000.019.8420261
TAGACAC4850.019.6452475
ATGGGTA22350.018.6508965
AAAGTAC21700.018.3844783
ATGGGAT21200.018.2581485
CCTAGAG3354.3780892E-717.7742563
GTCGGGA2702.4374627E-517.638522
GTAGCCC5951.8189894E-1217.0125033
AAAAAGT23750.016.5419851
Produced by FastQC (version 0.11.5)