##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523078 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.279791973884464 31.0 31.0 34.0 26.0 34.0 2 30.396357244999926 31.0 31.0 34.0 26.0 34.0 3 30.487573847170008 31.0 31.0 34.0 26.0 34.0 4 33.90152047367239 37.0 35.0 37.0 28.0 37.0 5 33.909105771339355 37.0 35.0 37.0 30.0 37.0 6 33.84894404620118 37.0 35.0 37.0 29.0 37.0 7 33.729902867745444 37.0 35.0 37.0 28.0 37.0 8 33.64455792809036 37.0 35.0 37.0 28.0 37.0 9 35.024383518112664 39.0 35.0 39.0 27.0 39.0 10-11 34.92715540504163 39.0 35.0 39.0 27.0 39.0 12-13 34.82229734787056 39.0 35.0 39.0 26.5 39.0 14-15 35.98022589782008 40.0 36.0 41.0 27.0 41.0 16-17 36.01629200868241 40.0 36.0 41.0 27.0 41.0 18-19 35.98811682658406 40.0 36.0 41.0 27.0 41.0 20-21 36.00563267278498 40.0 36.0 41.0 27.0 41.0 22-23 35.98927894697448 40.0 36.0 41.0 27.0 41.0 24-25 35.991442985848394 40.0 36.0 41.0 27.0 41.0 26-27 35.85685138909498 40.0 36.0 41.0 27.0 41.0 28-29 35.76892943106 40.0 36.0 41.0 26.0 41.0 30-31 35.837418700815064 40.0 36.0 41.0 27.0 41.0 32-33 35.58178011894336 40.0 35.0 41.0 26.0 41.0 34-35 35.65459713816364 40.0 35.0 41.0 27.0 41.0 36-37 35.65186352898539 40.0 35.0 41.0 27.0 41.0 38-39 35.53633366117822 40.0 35.0 41.0 26.0 41.0 40-41 35.27959795887013 39.0 35.0 41.0 25.0 41.0 42-43 35.09461728158374 39.0 35.0 41.0 25.0 41.0 44-45 34.88611942395596 39.0 34.0 41.0 24.0 41.0 46-47 34.66573904947744 39.0 34.0 41.0 24.0 41.0 48-49 34.43716802422463 39.0 33.0 41.0 23.0 41.0 50-51 34.30423622427742 39.0 33.0 41.0 22.5 41.0 52-53 34.147651663276605 38.0 33.0 40.5 22.0 41.0 54-55 33.88036364519742 38.0 33.0 40.0 20.0 41.0 56-57 33.53916050261378 38.0 32.5 40.0 18.0 41.0 58-59 33.23380253670528 38.0 32.0 40.0 13.5 41.0 60-61 32.95997775557129 37.0 32.0 40.0 13.0 41.0 62-63 32.48340236022055 37.0 31.0 40.0 8.0 41.0 64-65 32.21741532606997 36.0 31.0 40.0 7.5 41.0 66-67 31.83147974036786 36.0 31.0 39.0 3.5 41.0 68-69 31.404129335464106 35.0 30.5 39.0 2.0 41.0 70-71 30.921912731980896 35.0 30.0 39.0 2.0 40.5 72-73 30.669670561849095 35.0 30.0 37.5 2.0 40.0 74-75 30.28298682011033 35.0 29.5 37.0 2.0 39.5 76-77 29.793813908414407 35.0 29.0 37.0 2.0 39.0 78-79 29.401111433557574 35.0 29.0 36.0 2.0 39.0 80-81 29.119877642510758 35.0 29.0 36.0 2.0 37.5 82-83 28.862995197882185 34.0 29.0 35.5 2.0 37.0 84-85 28.578791105905278 34.0 29.0 35.0 2.0 37.0 86-87 28.218654264587894 34.0 28.0 35.0 2.0 36.0 88-89 27.95430437574438 34.0 27.0 35.0 2.0 36.0 90-91 27.77594417357483 34.0 27.0 35.0 2.0 36.0 92-93 27.478252919417127 34.0 26.5 35.0 2.0 35.5 94-95 27.386549802439532 34.0 26.0 35.0 2.0 35.0 96-97 27.287344443291808 34.0 26.0 35.0 2.0 35.0 98-99 27.07782398537698 34.0 25.5 35.0 2.0 35.0 100-101 26.710233487713694 34.0 25.0 35.0 2.0 35.0 102-103 25.604676516895392 32.5 21.5 34.5 2.0 35.0 104-105 26.281208841569505 34.0 24.0 35.0 2.0 35.0 106-107 26.325469214314698 34.0 23.5 35.0 2.0 35.0 108-109 26.268754784718837 34.0 23.5 35.0 2.0 35.0 110-111 26.284096087002766 34.0 24.0 35.0 2.0 35.0 112-113 26.18312029981393 34.0 23.5 35.0 2.0 35.0 114-115 26.04370262061431 34.0 22.0 35.0 2.0 35.0 116-117 25.78834209410155 34.0 19.5 35.0 2.0 35.0 118-119 25.60893860984139 34.0 18.5 35.0 2.0 35.0 120-121 25.427694116781936 34.0 11.0 35.0 2.0 35.0 122-123 25.122792463682096 34.0 2.0 35.0 2.0 35.0 124-125 24.219504844794557 32.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6079829654438029 1101 2 0.6181951091425759 1101 3 0.6366989316425453 1101 4 0.7561298160324199 1101 5 0.5749477512511731 1101 6 0.5933009107879599 1101 7 0.7145208881044738 1101 8 0.7207813234012193 1101 9 0.9502275185170888 1101 10-11 0.8669902694885252 1101 12-13 0.8430907316490703 1101 14-15 0.9624697169675756 1101 16-17 0.8756007201840461 1101 18-19 0.8994613137047338 1101 20-21 0.9115439281712767 1101 22-23 0.9270750317005323 1101 24-25 0.8601670368119798 1101 26-27 0.9224740963269724 1101 28-29 1.0916379542984203 1101 30-31 0.927802428943231 1101 32-33 1.0294827204900372 1101 34-35 0.9394322422859887 1101 36-37 0.9855652939697421 1101 38-39 0.9969797834861325 1101 40-41 1.0127888461080161 1101 42-43 0.9685352001255367 1101 44-45 1.061722982531208 1101 46-47 1.0817414662581655 1101 48-49 1.1402897744654865 1101 50-51 1.0922771629460328 1101 52-53 1.1187214844695532 1101 54-55 1.243028016196348 1101 56-57 1.23223331649789 1101 58-59 1.2915393404109352 1101 60-61 1.2240159016861583 1101 62-63 1.3221139603159173 1101 64-65 1.2662312319468754 1101 66-67 1.178822866818546 1101 68-69 1.2538298504917371 1101 70-71 1.1757855329912132 1101 72-73 1.1773618753957251 1101 74-75 1.1063678265691337 1101 76-77 1.157481357797078 1101 78-79 1.1828724658895773 1101 80-81 1.0551748307592312 1101 82-83 1.1300982518554008 1101 84-85 1.1616352134548826 1101 86-87 1.0954321220275212 1101 88-89 1.186623254245216 1101 90-91 1.1613570424980075 1101 92-93 1.1539307734879607 1101 94-95 1.2725069852684179 1101 96-97 1.2656427013489768 1101 98-99 1.1340026063089503 1101 100-101 1.0302693520482862 1101 102-103 0.9690592554738444 1101 104-105 1.0758702074624864 1101 106-107 1.000684712707173 1101 108-109 1.0467831325580086 1101 110-111 1.0982904707173553 1101 112-113 1.1429326395001915 1101 114-115 1.148953031100035 1101 116-117 1.1198642755630068 1101 118-119 1.1209880196918824 1101 120-121 1.1024390619761242 1101 122-123 1.144576069516134 1101 124-125 1.2331249089278131 1102 1 0.4219951377284481 1102 2 0.4948700987389145 1102 3 0.49476593122639656 1102 4 0.5307011851543493 1102 5 0.4559777137979921 1102 6 0.4480122091649861 1102 7 0.5408796063733092 1102 8 0.5287266001386328 1102 9 0.5839244598404392 1102 10-11 0.5371314992013865 1102 12-13 0.5116209542874373 1102 14-15 0.5517435413537584 1102 16-17 0.5621063901798777 1102 18-19 0.584933119780473 1102 20-21 0.5826761066981234 1102 22-23 0.6977660949547939 1102 24-25 0.7034936077649547 1102 26-27 0.7561208399807384 1102 28-29 0.8414374237116604 1102 30-31 0.6493437314817214 1102 32-33 0.8579351757542923 1102 34-35 0.7868144208128314 1102 36-37 0.6598835940113617 1102 38-39 0.7736552932655698 1102 40-41 0.7883529846847992 1102 42-43 0.8552728717859566 1102 44-45 0.8541314303048182 1102 46-47 0.8109671737071906 1102 48-49 0.8261889109615339 1102 50-51 0.8123263202494044 1102 52-53 0.814639607648914 1102 54-55 0.8377195476154142 1102 56-57 0.7046022303555688 1102 58-59 0.8148607095328657 1102 60-61 0.8602411929886955 1102 62-63 0.9170416548644056 1102 64-65 0.825564617298518 1102 66-67 0.8231859026071398 1102 68-69 0.8617147859890331 1102 70-71 0.8444260344477286 1102 72-73 0.6948571937477865 1102 74-75 0.6799492872432893 1102 76-77 0.8515533511387332 1102 78-79 0.8373356369257827 1102 80-81 0.7351680683788757 1102 82-83 0.704226164880783 1102 84-85 0.7297976666384045 1102 86-87 0.7716126775830752 1102 88-89 0.8632103328969016 1102 90-91 0.7497725647618445 1102 92-93 0.7146759357850883 1102 94-95 0.6537106889721223 1102 96-97 0.5508661199923388 1102 98-99 0.419199755705538 1102 100-101 0.32988389636788895 1102 102-103 0.23993654427950162 1102 104-105 0.3722981100842091 1102 106-107 0.4312283040513236 1102 108-109 0.41585486580811093 1102 110-111 0.37453865007649156 1102 112-113 0.35473689459798763 1102 114-115 0.3976595958108149 1102 116-117 0.358859021713652 1102 118-119 0.24684918282130397 1102 120-121 0.27929611565902235 1102 122-123 0.27318822099345397 1102 124-125 0.2671979426356792 1103 1 0.41641663119806793 1103 2 0.41221819641069146 1103 3 0.4299760391196408 1103 4 0.42569550656264 1103 5 0.4620793605895841 1103 6 0.40802143687651693 1103 7 0.3142299754817657 1103 8 0.4024645331312513 1103 9 0.571890814184556 1103 10-11 0.510465897270663 1103 12-13 0.41066446649640653 1103 14-15 0.5216895947325213 1103 16-17 0.3936655185160518 1103 18-19 0.44769788411891653 1103 20-21 0.5281605615533209 1103 22-23 0.6047752516839253 1103 24-25 0.6623543403032812 1103 26-27 0.8269843408325244 1103 28-29 0.8557677048019414 1103 30-31 0.5235508581143193 1103 32-33 0.7329500820575277 1103 34-35 0.7269035037202656 1103 36-37 0.7107236706723512 1103 38-39 0.6628184108124202 1103 40-41 0.7221821300567441 1103 42-43 0.6721656173850477 1103 44-45 0.6255950873178762 1103 46-47 0.5995287154448334 1103 48-49 0.6070254385027027 1103 50-51 0.7008786342755258 1103 52-53 0.691931629227561 1103 54-55 0.8445019155881468 1103 56-57 0.8544929174651585 1103 58-59 0.7793900962449598 1103 60-61 0.8017754774861459 1103 62-63 0.8830815469711553 1103 64-65 0.8678789983320243 1103 66-67 0.8634368253782974 1103 68-69 0.8188939455574591 1103 70-71 0.8697159975368791 1103 72-73 0.8411724975524422 1103 74-75 0.6809352327288103 1103 76-77 0.5988503414851287 1103 78-79 0.6440647681012486 1103 80-81 0.6106983068797263 1103 82-83 0.6287331921380215 1103 84-85 0.6494182657298282 1103 86-87 0.4880120955274272 1103 88-89 0.4596917355090504 1103 90-91 0.523345818594894 1103 92-93 0.4323750414068961 1103 94-95 0.5425792295740521 1103 96-97 0.512518235267752 1103 98-99 0.48735634287191587 1103 100-101 0.49323355565238813 1103 102-103 0.5554877225872836 1103 104-105 0.5388150038945447 1103 106-107 0.44372191563565266 1103 108-109 0.44569976927205346 1103 110-111 0.4814903110645652 1103 112-113 0.5171747850011634 1103 114-115 0.4789170489624297 1103 116-117 0.4249901261997415 1103 118-119 0.3781818773130716 1103 120-121 0.3631760134443702 1103 122-123 0.2026118439349638 1103 124-125 0.3054759096998474 1104 1 0.21236412434956975 1104 2 0.34296289691711124 1104 3 0.2094300359801693 1104 4 0.27452555179255 1104 5 0.10822223415096488 1104 6 0.15033678929592753 1104 7 0.1426330934214164 1104 8 0.1245940923105664 1104 9 0.3731185247422317 1104 10-11 0.3040349649002181 1104 12-13 0.2265698939401517 1104 14-15 0.3410190893184577 1104 16-17 0.31588396632562166 1104 18-19 0.21458690571841998 1104 20-21 0.2918813799688351 1104 22-23 0.3009252537933875 1104 24-25 0.32109928667187404 1104 26-27 0.29130177507894217 1104 28-29 0.34194286777655947 1104 30-31 0.24557167056512696 1104 32-33 0.3500690357030578 1104 34-35 0.3123686123869831 1104 36-37 0.2479050500318536 1104 38-39 0.34494372195899103 1104 40-41 0.42071510395607703 1104 42-43 0.3498183101577155 1104 44-45 0.3777060104357588 1104 46-47 0.25469102084637996 1104 48-49 0.4115157327662615 1104 50-51 0.47644975463597206 1104 52-53 0.5097566728353655 1104 54-55 0.5783312043190776 1104 56-57 0.4856220140162506 1104 58-59 0.5519646804978464 1104 60-61 0.48616828521755906 1104 62-63 0.468913630383895 1104 64-65 0.4651933178393648 1104 66-67 0.4121346287671166 1104 68-69 0.49235170600041656 1104 70-71 0.39816945175487106 1104 72-73 0.3823596844964321 1104 74-75 0.5067842573543047 1104 76-77 0.37028235768584494 1104 78-79 0.3170751757814614 1104 80-81 0.39818792320381036 1104 82-83 0.43842823576037304 1104 84-85 0.4600956669230598 1104 86-87 0.43891822127792324 1104 88-89 0.47730761442266 1104 90-91 0.49135027611965754 1104 92-93 0.47026891187390163 1104 94-95 0.5207595076456215 1104 96-97 0.4260765882530855 1104 98-99 0.4092463682873735 1104 100-101 0.3326560996129757 1104 102-103 0.23064978794588598 1104 104-105 0.22802576451062606 1104 106-107 0.29567215546037673 1104 108-109 0.27338457688126994 1104 110-111 0.2980105757810172 1104 112-113 0.3431380118766647 1104 114-115 0.4871456120362616 1104 116-117 0.319527076083137 1104 118-119 0.19657502255942205 1104 120-121 0.12298740515517892 1104 122-123 0.16618423579561892 1104 124-125 0.12485450397925746 1105 1 0.1775012178428952 1105 2 0.27419770961159173 1105 3 0.2954025549746113 1105 4 0.22607314509712495 1105 5 0.24482606859054812 1105 6 0.21124361116785195 1105 7 0.27876229592981616 1105 8 0.11563289484535488 1105 9 0.40211651992627395 1105 10-11 0.32424970377935125 1105 12-13 0.3864376567194796 1105 14-15 0.34083510072428425 1105 16-17 0.23358260620276639 1105 18-19 0.3902567964614434 1105 20-21 0.3180328663547769 1105 22-23 0.42114860568440804 1105 24-25 0.37966850046881717 1105 26-27 0.4277544236717219 1105 28-29 0.3881139258575743 1105 30-31 0.24497499042306714 1105 32-33 0.27517230021781103 1105 34-35 0.423460522386506 1105 36-37 0.3376491519512683 1105 38-39 0.3684460657977624 1105 40-41 0.36827340671626985 1105 42-43 0.5044350677802285 1105 44-45 0.43517935591282964 1105 46-47 0.4886576243509637 1105 48-49 0.4908661284150426 1105 50-51 0.48893129163710114 1105 52-53 0.5552529839007079 1105 54-55 0.6041090325939464 1105 56-57 0.5456143905844684 1105 58-59 0.43437251210797 1105 60-61 0.4929960213148803 1105 62-63 0.5305305675968768 1105 64-65 0.5229135085717687 1105 66-67 0.4051324333653632 1105 68-69 0.41962966438674343 1105 70-71 0.5138850830886419 1105 72-73 0.382108624362953 1105 74-75 0.4231735361703315 1105 76-77 0.4209882581487463 1105 78-79 0.3803613879987431 1105 80-81 0.3677378108681424 1105 82-83 0.35466339664044 1105 84-85 0.32530642915628505 1105 86-87 0.41271872193214776 1105 88-89 0.3053008193060549 1105 90-91 0.26313093386055897 1105 92-93 0.3075863435104118 1105 94-95 0.2272121195872856 1105 96-97 0.17396071942009073 1105 98-99 0.1327208572792138 1105 100-101 0.2497926946511484 1105 102-103 0.17638414799910151 1105 104-105 0.17761105715144865 1105 106-107 0.15994611521012203 1105 108-109 0.1900403092988121 1105 110-111 0.16984712647133904 1105 112-113 0.2170535970300307 1105 114-115 0.16553244988806526 1105 116-117 0.26298008752194946 1105 118-119 0.19772275220614333 1105 120-121 0.21465019291223797 1105 122-123 0.3150837860864435 1105 124-125 0.39408277811493164 1106 1 0.029690162504127926 1106 2 -0.04133587420871976 1106 3 -0.024748599374913027 1106 4 0.10144512515702786 1106 5 0.06462442599833906 1106 6 0.14594427692981782 1106 7 0.20662756405376825 1106 8 0.13956997766641877 1106 9 0.12847196888021273 1106 10-11 0.13836434036918632 1106 12-13 0.16629443426600687 1106 14-15 0.0767569336130336 1106 16-17 0.14461501144678834 1106 18-19 0.1600236849338188 1106 20-21 0.13878624129032602 1106 22-23 0.14675690451687018 1106 24-25 0.18853127349416354 1106 26-27 0.45459035585056284 1106 28-29 0.39910943054170644 1106 30-31 0.290180245476634 1106 32-33 -0.074854599255751 1106 34-35 0.19084758339485575 1106 36-37 0.31484115728978423 1106 38-39 0.3105159802884714 1106 40-41 0.29564163809312305 1106 42-43 0.3948569584338699 1106 44-45 0.2761861376839505 1106 46-47 0.34795466868509095 1106 48-49 0.3729023668340403 1106 50-51 0.3604375931836472 1106 52-53 0.3759867015286531 1106 54-55 0.5124357991220378 1106 56-57 0.6172682943705681 1106 58-59 0.5178644258848095 1106 60-61 0.4776786678782088 1106 62-63 0.35940120354526073 1106 64-65 0.516075783094692 1106 66-67 0.6444006642249285 1106 68-69 0.5798040453970934 1106 70-71 0.4540901398448085 1106 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47954.0 3 8322.0 4 5330.0 5 3768.0 6 4062.0 7 4261.0 8 4761.0 9 5794.0 10 7044.0 11 8338.0 12 9505.0 13 10725.0 14 11682.0 15 11498.0 16 11680.0 17 12111.0 18 12436.0 19 13051.0 20 13640.0 21 14388.0 22 15671.0 23 18036.0 24 20444.0 25 23088.0 26 25995.0 27 27861.0 28 31206.0 29 37127.0 30 42136.0 31 47780.0 32 57981.0 33 69355.0 34 85448.0 35 110770.0 36 181421.0 37 335283.0 38 172000.0 39 1126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.8002715437221 18.13227266789728 10.908919380014751 23.15853640836587 2 13.925172055984078 22.737710357529465 37.93319089061441 25.40392669587204 3 21.12189932335682 29.765902170020205 27.87850366814606 21.233694838476914 4 13.071802309667454 18.060560010922412 34.53169840167424 34.335939277735896 5 11.750831246285692 41.380004049872795 33.37180932354592 13.49735538029559 6 30.699527834542884 32.0835702515973 20.48101773489869 16.73588417896113 7 26.20375945849868 33.50898841797644 22.131551368559762 18.155700754965114 8 28.310540239212038 33.59286454107045 21.764864989675488 16.331730230042023 9 30.147510492398826 15.980842176243272 19.591573355080193 34.280073976277706 10-11 23.86469770635104 29.409631920198187 25.89296524803298 20.832705125417792 12-13 26.093384056108686 26.80421112085083 25.515808198759732 21.58659662428075 14-15 25.986500776086636 26.380052231653124 23.824078673350314 23.80936831890993 16-17 22.738118405298522 29.433865802050935 28.208681999742723 19.61933379290782 18-19 24.02691735001624 28.462080075983305 28.243001472506347 19.268001101494107 20-21 22.180294524395112 28.940324986749406 28.217289332008328 20.662091156847154 22-23 21.23681834510956 30.046404439414115 24.42248459535573 24.294292620120597 24-25 21.51389993838668 31.15796757846463 23.72990145826984 23.598231024878853 26-27 23.220448342449764 27.780830882969017 24.51527648488119 24.483444289700028 28-29 22.907832649702055 27.807118671462806 26.683093608830966 22.601955070004173 30-31 24.675390226160452 29.179762177711872 25.56119495060627 20.58365264552141 32-33 20.449399134098595 29.519887889388418 27.42319116733063 22.607521809182355 34-35 19.194878744255213 30.354510919548574 27.86625779661442 22.584352539581797 36-37 23.55987520270328 28.53607178680264 26.052227048778704 21.851825961715377 38-39 25.295312768836965 27.375208559801866 25.42887014807993 21.90060852328124 40-41 22.009788104575637 30.040364642985327 25.69056102799817 22.259286224440864 42-43 24.177522231645522 28.72876749715131 25.182350748273397 21.911359522929768 44-45 25.275221674292116 27.464102810973785 25.19091092538054 22.069764589353564 46-47 22.558871042467477 28.45954449032729 28.888812680063957 20.092771787141277 48-49 23.916193214604856 28.06547982154457 27.96537067751732 20.052956286333256 50-51 21.047920419687767 28.991576967813913 28.3659818694463 21.59452074305202 52-53 20.435039181878423 30.030989453695494 24.888948963007163 24.64502240141892 54-55 21.12014226540648 30.71801303176443 24.017013033083167 24.14483166974592 56-57 22.919636040963194 27.762886040633568 24.654321980599576 24.66315593780367 58-59 22.770593137203193 27.470141401462012 26.643277760783114 23.11598770055168 60-61 24.109573596209042 28.90978933327883 25.7975579249417 21.183079145570435 62-63 20.259374107820456 29.23859872838247 27.439832894977158 23.06219426881992 64-65 18.906357314949666 29.92729249873963 27.922964537823823 23.24338564848688 66-67 22.893456191359434 28.518829765103092 26.113418719419702 22.474295324117772 68-69 24.465398126367184 27.24818628848934 25.688023487172558 22.59839209797092 70-71 21.451854772977264 29.45439629863124 25.850989045193522 23.24275988319797 72-73 23.52264656481653 28.38646646588557 25.471359677554435 22.619527291743466 74-75 24.49730864754428 27.108105184991736 25.48831461059474 22.90627155686925 76-77 21.942935745756547 27.97973073891012 29.05250864912147 21.024824866211862 78-79 23.20808592048788 27.79235156795264 28.049833460975336 20.94972905058415 80-81 20.612697006974397 28.736797923870185 28.331614984877763 22.31889008427766 82-83 20.065584602059523 29.474792361035785 25.226366117831382 25.23325691907331 84-85 20.607820400934106 30.13919573817525 24.469192404915553 24.78379145597509 86-87 22.18612909447904 27.061339083614833 25.41980516964814 25.332726652257993 88-89 22.21261394082874 26.925591351623645 27.010681236674827 23.851113470872786 90-91 23.240961082856767 28.330633180819227 26.28326479100028 22.145140945323725 92-93 20.013940471417417 28.55920545907278 27.44261590242461 23.984238167085188 94-95 18.78386549878435 28.993548863583165 28.20682410613547 24.015761531497017 96-97 22.500182936355948 27.598668618866775 26.690084717661634 23.21106372711565 98-99 23.76262550537446 26.687824648202696 26.073252398726567 23.476297447696275 100-101 21.056067350416985 28.320628899149686 26.29880486489978 24.32449888553355 102-103 22.752665725706017 27.430189022389328 26.30199170004178 23.515153551862877 104-105 23.46904214190778 26.294097839760195 26.39000819076374 23.84685182756828 106-107 21.215572370766843 27.280384530972263 29.327835326047914 22.176207772212983 108-109 22.199698717456492 27.332080790650814 28.393226778224477 22.074993713668214 110-111 19.84270286590487 28.30372764081327 28.772204269390045 23.081365223891815 112-113 19.439198368893486 28.95240430663801 25.794706286787072 25.813691037681437 114-115 19.782857609937356 29.951259802916635 25.18689565023075 25.07898693691526 116-117 21.081477200348353 27.27595343293851 25.858321122401804 25.784248244311332 118-119 21.03038964242165 27.083785354066602 27.194083908677413 24.691741094834335 120-121 21.828659728338774 28.358230610972303 26.61717438954377 23.195935271145153 122-123 19.34907265827633 28.551294098956227 27.473073321451984 24.62655992131546 124-125 18.475695771974003 29.186233536802416 27.921154632107687 24.4169160591159 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6626.0 1 3780.0 2 1305.0 3 1812.0 4 1855.0 5 1596.5 6 1283.5 7 1107.0 8 1044.5 9 976.5 10 950.5 11 912.5 12 905.0 13 959.5 14 1029.5 15 1099.5 16 1205.5 17 1362.5 18 1528.5 19 1807.0 20 2139.5 21 2604.5 22 3294.5 23 4149.0 24 5202.5 25 6568.0 26 8290.0 27 10136.0 28 11700.5 29 13488.0 30 16458.5 31 19205.0 32 21531.5 33 24472.0 34 27855.5 35 30350.0 36 32889.5 37 35221.5 38 35406.0 39 35318.0 40 35430.0 41 36528.0 42 37386.0 43 39010.0 44 41799.0 45 43501.0 46 45138.5 47 49529.5 48 58263.0 49 80975.0 50 147837.5 51 155198.5 52 84902.5 53 49429.0 54 38269.5 55 32067.0 56 27642.0 57 23983.0 58 21607.0 59 19425.0 60 16574.0 61 13525.5 62 11250.5 63 9224.5 64 7210.5 65 5645.5 66 4120.0 67 3004.0 68 2189.5 69 1435.0 70 1111.0 71 872.0 72 725.0 73 664.0 74 482.0 75 351.5 76 253.0 77 128.0 78 72.0 79 48.5 80 32.0 81 18.5 82 12.0 83 14.0 84 14.0 85 12.0 86 9.0 87 6.5 88 5.5 89 4.0 90 5.0 91 4.0 92 3.0 93 4.5 94 3.0 95 2.5 96 2.0 97 1.5 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4792925903991785 2 0.46924714295656555 3 0.4541461435330298 4 0.454934021763823 5 0.45834816076392676 6 0.46511078224490143 7 0.4496815002252018 8 0.47719158178373006 9 0.4896006639187224 10-11 0.4939668224477013 12-13 0.4937041963707703 14-15 0.46872189080270343 16-17 0.47161077764894505 18-19 0.4566739195234913 20-21 0.46671936696610417 22-23 0.4847092532358816 24-25 0.4708885559373847 26-27 0.4804087512261355 28-29 0.4776840056779758 30-31 0.4891738965437095 32-33 0.4879592509379034 34-35 0.48296935547621334 36-37 0.43983302234028726 38-39 0.4022774933391461 40-41 0.3960729522716499 42-43 0.4039517345795816 44-45 0.4375350441671405 46-47 0.4449214025808264 48-49 0.4420653439942013 50-51 0.42821181843608797 52-53 0.42236838822437195 54-55 0.4251916185513808 56-57 0.407333045320069 58-59 0.41114112343556924 60-61 0.4213178839166477 62-63 0.4219744491089754 64-65 0.4372395898305931 66-67 0.42522444681099725 68-69 0.42712848586874735 70-71 0.437666357205606 72-73 0.4377320137248388 74-75 0.4425249396288306 76-77 0.43297191608046337 78-79 0.42456788161866954 80-81 0.4334971682343255 82-83 0.4309365639842477 84-85 0.42968909011882517 86-87 0.43533555077284286 88-89 0.4232547512340143 90-91 0.4265704054552689 92-93 0.4353027225132265 94-95 0.4333986834554764 96-97 0.40424718891612904 98-99 0.3783456920788036 100-101 0.3781815507807217 102-103 0.35979772539554766 104-105 0.36209570356869447 106-107 0.38087346806926503 108-109 0.38720932217522674 110-111 0.403131028089172 112-113 0.3986007282621113 114-115 0.39755022395438716 116-117 0.3711891314824323 118-119 0.4424592831095978 120-121 0.4474163503116715 122-123 0.4027699172333919 124-125 0.3977471935120854 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1523078.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.97649082699515 #Duplication Level Percentage of deduplicated Percentage of total 1 87.59049746146626 49.03008677683708 2 8.389619425834228 9.392429096643799 3 1.8573902582851811 3.1191056626515183 4 0.663978933063784 1.4866884262385172 5 0.34563199922809823 0.9673633217153819 6 0.22004590199052523 0.7390438448574306 7 0.14700378531869007 0.5760129228297652 8 0.10545586321739334 0.47224393280330185 9 0.07906074164543804 0.3982988591542166 >10 0.5219411949217846 5.689287931014062 >50 0.0500641040305437 1.9147246813423093 >100 0.02387639337512971 2.4304325686505446 >500 0.0013173182116423426 0.5240988819915686 >1k 0.0018113125410082211 3.415491395040023 >5k 9.87988658731757E-4 3.6292418887682065 >10k+ 0.0013173182116423426 16.215449809462314 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 52002 3.414270313142203 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 37772 2.4799780444599686 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 25202 1.654675597704123 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 13686 0.8985751222196106 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 13027 0.855307476045219 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 12246 0.8040297345244302 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 11548 0.7582014840999607 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10440 0.6854540607900581 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8731 0.573247069421264 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 8333 0.5471157747666239 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 5847 0.3838936679539722 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5663 0.37181286841514355 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 5583 0.3665603468765224 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5217 0.34253006083733073 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4961 0.3257219919137431 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 4764 0.3127876576248885 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 4573 0.30024726245143063 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4350 0.2856058586625242 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3972 0.2607876943925393 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3598 0.23623215619948554 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3379 0.22185337848751016 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2559 0.16801503271664353 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 1938 0.12724233427309697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.3131303846552835E-4 0.0 0.0 0.0 0.0 8 1.3131303846552835E-4 0.0 0.0 0.0 0.0 9 1.3131303846552835E-4 0.0 0.0 0.0 0.0 10-11 1.3131303846552835E-4 0.0 0.0 6.565651923276418E-5 0.0 12-13 1.3131303846552835E-4 0.0 0.0 3.282825961638209E-4 0.0 14-15 1.9696955769829254E-4 0.0 0.0 7.22221711560406E-4 0.0 16-17 1.9696955769829254E-4 0.0 0.0 0.0019368673173665433 0.0 18-19 2.626260769310567E-4 0.0 0.0 0.002888886846241624 0.0 20-21 2.626260769310567E-4 0.0 0.0 0.004333330269362436 0.0 22-23 2.626260769310567E-4 0.0 0.0 0.006893934519440238 0.0 24-25 3.282825961638209E-4 0.0 0.0 0.010439386558009505 0.0 26-27 3.282825961638209E-4 0.0 0.0 0.013393929923483892 0.0 28-29 4.5959563462934927E-4 0.0 0.0 0.018088371048626532 0.0 30-31 4.5959563462934927E-4 0.0 0.0 0.02908583802011453 0.0 32-33 4.5959563462934927E-4 0.0 0.0 0.04792925903991785 0.0 34-35 4.5959563462934927E-4 0.0 0.0 0.07842671222353681 0.0 36-37 4.5959563462934927E-4 0.0 0.0 0.1228105192248854 0.0 38-39 5.909086730948776E-4 0.0 0.0 0.18909077539036084 0.0 40-41 5.909086730948776E-4 0.0 0.0 0.2863937368933174 0.0 42-43 6.237369327112597E-4 0.0 0.0 0.36659317513613876 0.0 44-45 6.565651923276418E-4 0.0 0.0 0.4524062457733616 0.0 46-47 7.878782307931702E-4 0.0 0.0 0.5634642480555823 0.0 48-49 7.878782307931702E-4 0.0 0.0 0.6706485157030697 0.0 50-51 7.878782307931702E-4 0.0 0.0 0.7814110636487429 0.0 52-53 7.878782307931702E-4 0.0 0.0 0.9107544065372883 0.0 54-55 7.878782307931702E-4 0.0 0.0 1.0441027970990322 0.0 56-57 7.878782307931702E-4 0.0 0.0 1.1999057172383818 0.0 58-59 7.878782307931702E-4 0.0 0.0 1.3831858906766428 0.0 60-61 8.535347500259343E-4 0.0 0.0 1.5517261755471485 0.0 62-63 8.535347500259343E-4 0.0 0.0 1.7203977734561198 0.0 64-65 8.535347500259343E-4 0.0 0.0 1.9216350049045419 0.0 66-67 9.191912692586985E-4 0.0 0.0 2.1476247441037164 0.0 68-69 9.191912692586985E-4 0.0 0.0 2.421379601044727 0.0 70-71 9.191912692586985E-4 0.0 0.0 2.7989045866331206 0.0 72-73 9.191912692586985E-4 0.0 0.0 3.1109371942868322 0.0 74-75 9.191912692586985E-4 0.0 0.0 3.41147991107481 0.0 76-77 9.191912692586985E-4 0.0 0.0 3.764055419354754 0.0 78-79 9.191912692586985E-4 0.0 0.0 4.128252131538897 0.0 80-81 9.848477884914627E-4 0.0 0.0 4.50705741925233 0.0 82-83 9.848477884914627E-4 0.0 0.0 4.949615187140777 0.0 84-85 9.848477884914627E-4 0.0 0.0 5.386001242221344 0.0 86-87 0.0010176760481078449 0.0 0.0 5.846154957264171 0.0 88-89 0.0010505043077242268 0.0 0.0 6.344750564317783 0.0 90-91 0.0010505043077242268 0.0 0.0 6.842952232255998 0.0 92-93 0.001116160826956991 0.0 0.0 7.357568030002403 0.0 94-95 0.001116160826956991 0.0 0.0 7.915845413038597 0.0 96-97 0.001116160826956991 0.0 0.0 8.48682733254633 0.0 98-99 0.001116160826956991 0.0 0.0 9.131278897075527 0.0 100-101 0.001116160826956991 0.0 0.0 9.98290960804371 0.0 102-103 0.001116160826956991 0.0 0.0 10.708085862969591 0.0 104-105 0.0011818173461897552 0.0 0.0 11.387007100095989 0.0 106-107 0.0011818173461897552 0.0 0.0 12.128367687012746 0.0 108-109 0.0011818173461897552 6.565651923276418E-5 0.0 12.827773758139767 0.0 110-111 0.0011818173461897552 6.565651923276418E-5 0.0 13.561321219267825 0.0 112-113 0.0011818173461897552 6.565651923276418E-5 0.0 14.41321455631294 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1160 0.0 34.90842 5 ATAACGC 175 4.4201443E-10 34.025005 3 TATAACG 250 2.1827873E-11 28.574402 2 TAACGCT 170 1.3911993E-5 24.518019 4 CATGGGT 4335 0.0 24.037271 4 AAGTACT 1735 0.0 24.023418 4 CATGGGG 5880 0.0 22.582071 4 ATGGGGG 2225 0.0 21.678751 5 ATGGGTG 2070 0.0 20.712921 5 GTATAGG 520 0.0 20.60518 1 ATGGGAG 1955 0.0 20.103718 5 GTGTAGC 600 0.0 19.842026 1 TAGACAC 485 0.0 19.645247 5 ATGGGTA 2235 0.0 18.650896 5 AAAGTAC 2170 0.0 18.384478 3 ATGGGAT 2120 0.0 18.258148 5 CCTAGAG 335 4.3780892E-7 17.774256 3 GTCGGGA 270 2.4374627E-5 17.63852 2 GTAGCCC 595 1.8189894E-12 17.012503 3 AAAAAGT 2375 0.0 16.541985 1 >>END_MODULE