##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364148_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1523078 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02860917169048 33.0 31.0 34.0 30.0 34.0 2 31.82463275025967 33.0 31.0 34.0 30.0 34.0 3 32.067282174648966 34.0 31.0 34.0 30.0 34.0 4 35.55526703162937 37.0 35.0 37.0 33.0 37.0 5 35.70037187852493 37.0 35.0 37.0 33.0 37.0 6 35.8296364335904 37.0 35.0 37.0 35.0 37.0 7 35.79876145542119 37.0 35.0 37.0 33.0 37.0 8 35.83732481199256 37.0 35.0 37.0 35.0 37.0 9 37.48122289206462 39.0 37.0 39.0 34.0 39.0 10-11 37.441945192564006 39.0 37.0 39.0 34.0 39.0 12-13 37.42721909186529 39.0 37.0 39.0 34.0 39.0 14-15 38.69083395597599 40.0 38.0 41.0 34.0 41.0 16-17 38.66287806665187 40.0 38.0 41.0 34.0 41.0 18-19 38.773920639652076 40.0 38.0 41.0 34.0 41.0 20-21 38.8248530278817 40.0 38.0 41.0 34.0 41.0 22-23 38.78610681790427 40.0 38.0 41.0 34.0 41.0 24-25 38.76233029431191 40.0 38.0 41.0 34.0 41.0 26-27 38.59764568853335 40.0 38.0 41.0 34.0 41.0 28-29 38.44907187944412 40.0 38.0 41.0 34.0 41.0 30-31 38.319353966113354 40.0 38.0 41.0 34.0 41.0 32-33 38.05134438288781 40.0 37.0 41.0 33.5 41.0 34-35 37.848701445362614 40.0 37.0 41.0 33.0 41.0 36-37 37.742711141517375 40.0 37.0 41.0 33.0 41.0 38-39 37.64055025415638 40.0 37.0 41.0 33.0 41.0 40-41 37.42763929358838 40.0 37.0 41.0 33.0 41.0 42-43 37.26286802120443 40.0 36.5 41.0 32.0 41.0 44-45 37.021171601191796 40.0 36.0 41.0 31.5 41.0 46-47 36.678745934220046 40.0 36.0 41.0 30.5 41.0 48-49 36.36521537308004 40.0 36.0 41.0 30.0 41.0 50-51 36.18951721448278 40.0 35.5 41.0 29.5 41.0 52-53 36.07980845367079 39.5 35.0 41.0 29.0 41.0 54-55 35.90068959042151 39.0 35.0 41.0 28.0 41.0 56-57 35.64695077993379 39.0 35.0 41.0 28.0 41.0 58-59 35.47475835118097 39.0 35.0 41.0 28.0 41.0 60-61 35.32922115610626 39.0 35.0 41.0 28.0 41.0 62-63 35.00209772578948 38.0 35.0 40.0 27.5 41.0 64-65 34.715832675673866 38.0 34.5 40.0 26.0 41.0 66-67 34.32465934114996 37.0 34.0 40.0 26.0 41.0 68-69 33.98923692680218 37.0 34.0 39.5 26.0 41.0 70-71 33.57638118336684 36.0 34.0 39.0 26.0 41.0 72-73 33.282235709530305 35.5 34.0 39.0 26.0 40.5 74-75 32.90881622608954 35.0 33.5 37.5 25.0 39.5 76-77 32.43882814931343 35.0 33.0 37.0 24.5 39.0 78-79 32.09192011177366 35.0 33.0 36.5 23.5 39.0 80-81 31.826719642723486 35.0 33.0 36.0 22.5 38.0 82-83 31.596837456781596 35.0 33.0 36.0 21.5 37.0 84-85 31.36217744593514 35.0 33.0 35.0 20.5 37.0 86-87 31.096842709303132 35.0 32.0 35.0 20.0 36.0 88-89 30.8817778866217 35.0 32.0 35.0 19.0 36.0 90-91 30.736155666354577 35.0 32.0 35.0 18.0 36.0 92-93 30.54561946269331 35.0 32.0 35.0 18.0 36.0 94-95 30.452580235549327 35.0 32.0 35.0 15.5 35.0 96-97 30.371548929207826 35.0 32.0 35.0 11.5 35.0 98-99 30.267690820824672 35.0 32.0 35.0 8.5 35.0 100-101 30.065011115648705 35.0 31.0 35.0 7.0 35.0 102-103 28.792341560970613 33.5 29.0 34.5 2.0 35.0 104-105 29.643855403334562 34.0 31.0 35.0 2.0 35.0 106-107 29.67011275850613 35.0 31.0 35.0 2.0 35.0 108-109 29.652823099013972 35.0 31.0 35.0 2.0 35.0 110-111 29.64610807850944 35.0 31.0 35.0 2.0 35.0 112-113 29.568879926044495 35.0 31.0 35.0 2.0 35.0 114-115 29.49054053699154 35.0 31.0 35.0 2.0 35.0 116-117 29.304598319980986 35.0 31.0 35.0 2.0 35.0 118-119 29.124730644129848 35.0 31.0 35.0 2.0 35.0 120-121 28.971693176580583 35.0 31.0 35.0 2.0 35.0 122-123 28.68997221416106 34.5 31.0 35.0 2.0 35.0 124-125 27.69798624889861 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34136406969167865 1101 2 0.6544136982590523 1101 3 0.4486570063282258 1101 4 0.5471584128935589 1101 5 0.2875400082440862 1101 6 0.20610251588301054 1101 7 0.2189729875899431 1101 8 0.2409361661743432 1101 9 0.1989425107804621 1101 10-11 0.28341093950730567 1101 12-13 0.26116507305058434 1101 14-15 0.4252179738363395 1101 16-17 0.40036417036213123 1101 18-19 0.30695247235751566 1101 20-21 0.283774929640046 1101 22-23 0.28912195325293055 1101 24-25 0.3547828727702296 1101 26-27 0.36175467073599066 1101 28-29 0.4173948884042744 1101 30-31 0.45624545152873 1101 32-33 0.44129061164754546 1101 34-35 0.5738746834058759 1101 36-37 0.4500193636175709 1101 38-39 0.37063401091139525 1101 40-41 0.45191764881512597 1101 42-43 0.4534265681919223 1101 44-45 0.5602048310343548 1101 46-47 0.49914251692862166 1101 48-49 0.48998801170409934 1101 50-51 0.6131324828589584 1101 52-53 0.5563359760568432 1101 54-55 0.5860391323008614 1101 56-57 0.6619514396098367 1101 58-59 0.5817843633262711 1101 60-61 0.5418405716457144 1101 62-63 0.500111832671891 1101 64-65 0.5012673951523112 1101 66-67 0.4762308030471445 1101 68-69 0.4632100034704649 1101 70-71 0.48346789301211857 1101 72-73 0.5009101855819935 1101 74-75 0.443901126785903 1101 76-77 0.42912888934665006 1101 78-79 0.447530506029274 1101 80-81 0.3956442183026532 1101 82-83 0.42987590278923804 1101 84-85 0.3989041344711737 1101 86-87 0.4767247619022612 1101 88-89 0.41243708888857 1101 90-91 0.5121625950283999 1101 92-93 0.5015680184376308 1101 94-95 0.5860627993227787 1101 96-97 0.5483924437601964 1101 98-99 0.5239188082717767 1101 100-101 0.5264463604225611 1101 102-103 0.36631876336500113 1101 104-105 0.573011572962109 1101 106-107 0.49760467316194124 1101 108-109 0.5416967813213738 1101 110-111 0.48594929661270925 1101 112-113 0.519245837994692 1101 114-115 0.5456088501788372 1101 116-117 0.6133425807879007 1101 118-119 0.5843361327771852 1101 120-121 0.6037799417993845 1101 122-123 0.7585939702340134 1101 124-125 0.8010506766974181 1102 1 0.28431246752771955 1102 2 0.6325129491953838 1102 3 0.4113687208517405 1102 4 0.5210285751906909 1102 5 0.2733577360842858 1102 6 0.24239569108068082 1102 7 0.269402658834224 1102 8 0.2418584508185333 1102 9 0.2972956110717675 1102 10-11 0.24285590413484925 1102 12-13 0.26639083251792073 1102 14-15 0.4232628656382573 1102 16-17 0.4362677284145704 1102 18-19 0.3166179946213532 1102 20-21 0.27576957175407557 1102 22-23 0.3256577938688281 1102 24-25 0.34012203215434056 1102 26-27 0.4196135970780688 1102 28-29 0.5318042204891711 1102 30-31 0.41532706825780963 1102 32-33 0.4460495067786283 1102 34-35 0.4774912564395848 1102 36-37 0.46627742645569015 1102 38-39 0.35063323063673835 1102 40-41 0.2815392156066281 1102 42-43 0.306186919835703 1102 44-45 0.42008284809636365 1102 46-47 0.3841438173863807 1102 48-49 0.4710265572721397 1102 50-51 0.357670560262207 1102 52-53 0.2694073971970852 1102 54-55 0.3082956866079769 1102 56-57 0.36191944834890677 1102 58-59 0.38163064921890566 1102 60-61 0.28852128325620185 1102 62-63 0.31340615227239255 1102 64-65 0.33608663152351426 1102 66-67 0.350730907083765 1102 68-69 0.37435622694112425 1102 70-71 0.26475300537167357 1102 72-73 0.22893427005973166 1102 74-75 0.22178944347338359 1102 76-77 0.17849894760716012 1102 78-79 0.2762875285011859 1102 80-81 0.26045877302591336 1102 82-83 0.34007773383376616 1102 84-85 0.322324598474502 1102 86-87 0.32741036323393047 1102 88-89 0.4434129523925243 1102 90-91 0.39577188549864317 1102 92-93 0.2828494895154492 1102 94-95 0.2012966216282308 1102 96-97 0.21993868803818017 1102 98-99 0.2192558869234631 1102 100-101 0.28234887810878817 1102 102-103 0.23314122487145283 1102 104-105 0.23657482722761003 1102 106-107 0.14692375347821596 1102 108-109 0.2033285957200448 1102 110-111 0.31313198491899286 1102 112-113 0.28429707603048016 1102 114-115 0.36476433290876287 1102 116-117 0.29462639268968616 1102 118-119 0.41848303248588437 1102 120-121 0.40858643368452974 1102 122-123 0.5703225386901103 1102 124-125 0.6249278614664568 1103 1 0.2522369422579871 1103 2 0.5889822848001494 1103 3 0.3684392773537226 1103 4 0.5127832600288542 1103 5 0.1926990194460103 1103 6 0.18348740033678723 1103 7 0.23753014321809474 1103 8 0.13783303912063616 1103 9 0.2596479676873287 1103 10-11 0.21728875478788723 1103 12-13 0.2775336490994036 1103 14-15 0.4357128230488172 1103 16-17 0.42872442915278697 1103 18-19 0.3180752632187378 1103 20-21 0.28367452632533485 1103 22-23 0.26459483248325455 1103 24-25 0.2552327646926642 1103 26-27 0.2090542557947046 1103 28-29 0.33853796552040194 1103 30-31 0.23886518580466287 1103 32-33 0.27457768678998207 1103 34-35 0.32754981691658713 1103 36-37 0.32268607211156564 1103 38-39 0.2980043975873272 1103 40-41 0.3407658623982286 1103 42-43 0.330537075996979 1103 44-45 0.3214056050526324 1103 46-47 0.3133349729797956 1103 48-49 0.2462469576128541 1103 50-51 0.22699196855295156 1103 52-53 0.1982904891902706 1103 54-55 0.24913753782887227 1103 56-57 0.2106847095641271 1103 58-59 0.17118097573792568 1103 60-61 0.1621349970551762 1103 62-63 0.17293269968863 1103 64-65 0.07489590182448325 1103 66-67 0.09235995308035427 1103 68-69 0.23447654210296065 1103 70-71 0.2660679542643436 1103 72-73 0.3164523705708078 1103 74-75 0.31318815159376356 1103 76-77 0.2811799527178991 1103 78-79 0.1284542661502499 1103 80-81 0.17340134826725873 1103 82-83 0.2225564391148538 1103 84-85 0.22173225037683153 1103 86-87 0.22466618379043268 1103 88-89 0.1800852976509013 1103 90-91 0.08164390423799972 1103 92-93 -0.058106288451611476 1103 94-95 0.045015744285809234 1103 96-97 0.03953444329087574 1103 98-99 0.028531866480530965 1103 100-101 0.029020513543198234 1103 102-103 -0.052823070983176734 1103 104-105 -0.0012573703873819397 1103 106-107 0.06115075518179225 1103 108-109 0.10648944183020959 1103 110-111 0.0700087594789025 1103 112-113 0.03532525604184045 1103 114-115 0.05455599957542745 1103 116-117 0.023828550554540584 1103 118-119 -0.030954786934898948 1103 120-121 -0.045644117138866847 1103 122-123 0.09038997196665832 1103 124-125 0.05016884670211752 1104 1 0.2794007111940999 1104 2 0.551187159272235 1104 3 0.024517382963892942 1104 4 0.444623792953287 1104 5 0.19071611923259013 1104 6 0.08445731931865197 1104 7 0.1453754740918356 1104 8 0.17703387068759469 1104 9 0.3213299122388591 1104 10-11 0.21043098241552372 1104 12-13 0.15741488603343612 1104 14-15 0.18749696042902997 1104 16-17 0.26305715340745195 1104 18-19 0.2497410376045579 1104 20-21 0.1995898464480632 1104 22-23 0.1735294491492212 1104 24-25 0.1386788212235075 1104 26-27 0.29543625577433374 1104 28-29 0.32068640807812443 1104 30-31 0.24667367372322957 1104 32-33 0.1274139033058148 1104 34-35 0.1746384596846795 1104 36-37 0.2993191642866009 1104 38-39 0.2504213390910479 1104 40-41 0.2295432363865899 1104 42-43 0.16657992439021285 1104 44-45 0.21187600921221872 1104 46-47 0.19906998448004032 1104 48-49 0.007144725504417693 1104 50-51 0.10400560153746596 1104 52-53 0.13897987798274158 1104 54-55 0.18628956648272066 1104 56-57 0.3702230706090859 1104 58-59 0.29662869220211263 1104 60-61 0.15329177855936393 1104 62-63 0.03730061569397236 1104 64-65 0.038206792354714025 1104 66-67 0.05192326404363001 1104 68-69 0.027016880228913465 1104 70-71 -0.09415231059758611 1104 72-73 -0.20514931234846756 1104 74-75 -0.18741832054598007 1104 76-77 -0.1696788918462957 1104 78-79 -0.20070062265167365 1104 80-81 -0.16422614727012785 1104 82-83 -0.14370108768630274 1104 84-85 -0.2270913209675669 1104 86-87 -0.12448901052069417 1104 88-89 -0.1724808705508316 1104 90-91 -0.21906425882244562 1104 92-93 -0.1897061965078919 1104 94-95 -0.11302180279534113 1104 96-97 -0.09544897919779771 1104 98-99 -0.17721294313631475 1104 100-101 -0.21015645920974535 1104 102-103 -0.29162197609637985 1104 104-105 -0.1642983580840891 1104 106-107 -0.11407859494194028 1104 108-109 -0.10327936044278019 1104 110-111 -0.030340830338619895 1104 112-113 -0.04949864699826989 1104 114-115 -0.13652000384045593 1104 116-117 -0.06450398021096504 1104 118-119 -0.19909595982837658 1104 120-121 -0.16064214587710168 1104 122-123 -0.1774204872136771 1104 124-125 0.08138370405114514 1105 1 0.16204494249195278 1105 2 0.5132103740880858 1105 3 0.14251857779022004 1105 4 0.3875092618859668 1105 5 0.07985272893120765 1105 6 0.1282147182623703 1105 7 0.13732469030775718 1105 8 0.1822214694165325 1105 9 0.26410176271697594 1105 10-11 0.1787983218744813 1105 12-13 0.1573815335193487 1105 14-15 0.2183460201446934 1105 16-17 0.3087766697593466 1105 18-19 0.16394810907056012 1105 20-21 0.16338312683275547 1105 22-23 0.1646697752708377 1105 24-25 0.18703190339897446 1105 26-27 0.35322590036848567 1105 28-29 0.30766884852923226 1105 30-31 0.2888061455110318 1105 32-33 0.22995419204329437 1105 34-35 0.22782655686877007 1105 36-37 0.2837693792453919 1105 38-39 0.21224535510025078 1105 40-41 0.29786879357516227 1105 42-43 0.21166885831924986 1105 44-45 0.2793219396557305 1105 46-47 0.24516938963245138 1105 48-49 0.3535721581305111 1105 50-51 0.43960904381012966 1105 52-53 0.3353220967679036 1105 54-55 0.49824919161512327 1105 56-57 0.4524943768181515 1105 58-59 0.49323919714451137 1105 60-61 0.45800089699010726 1105 62-63 0.49226702494763686 1105 64-65 0.4960213597066385 1105 66-67 0.5330860572983553 1105 68-69 0.49773337286386976 1105 70-71 0.4401419538695279 1105 72-73 0.46719339023140094 1105 74-75 0.5343207381800994 1105 76-77 0.43301075018226953 1105 78-79 0.477964030647108 1105 80-81 0.442426763011607 1105 82-83 0.4219876050783995 1105 84-85 0.395794734382946 1105 86-87 0.33618361073035175 1105 88-89 0.4201583028932383 1105 90-91 0.30641834472611507 1105 92-93 0.34956712184735395 1105 94-95 0.3583754842891729 1105 96-97 0.39000932466193206 1105 98-99 0.4295777753354777 1105 100-101 0.36745080943925856 1105 102-103 0.242541260636834 1105 104-105 0.33049113624048587 1105 106-107 0.25056645734087724 1105 108-109 0.33359862433234966 1105 110-111 0.29916918091327105 1105 112-113 0.24070176129514564 1105 114-115 0.10305385365268194 1105 116-117 0.2578953483406181 1105 118-119 0.22962037154167803 1105 120-121 0.23076096873459662 1105 122-123 0.15342160879025357 1105 124-125 0.20674206032196452 1106 1 0.23093828780359615 1106 2 0.49321302954893653 1106 3 0.39713726913083036 1106 4 0.4787505518881616 1106 5 0.15578977036846453 1106 6 0.14464391660632714 1106 7 0.09527081204166876 1106 8 0.0074609350826335685 1106 9 0.2705803974652312 1106 10-11 0.17648169093008192 1106 12-13 0.1303767036843837 1106 14-15 0.24509668444098764 1106 16-17 0.21039572493258163 1106 18-19 0.11022503225359515 1106 20-21 0.07295476995950168 1106 22-23 0.019000418082036674 1106 24-25 0.0741477452921444 1106 26-27 0.09758132173321599 1106 28-29 0.28399644963072035 1106 30-31 0.2518198866587795 1106 32-33 0.2533656143184686 1106 34-35 0.1472695308469767 1106 36-37 0.14713143408927465 1106 38-39 0.20291753156080006 1106 40-41 0.17015905958680833 1106 42-43 0.21682062480423525 1106 44-45 0.16388465939942876 1106 46-47 0.13296220161030448 1106 48-49 0.19470800564498347 1106 50-51 0.2523237007469987 1106 52-53 0.13421982186562786 1106 54-55 0.21396831283001916 1106 56-57 0.23327901377009397 1106 58-59 0.26850280336380905 1106 60-61 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66-67 -1.606636635213654 2216 68-69 -1.6220679716341166 2216 70-71 -1.6394572573175523 2216 72-73 -1.4504885928075915 2216 74-75 -1.3273488393998747 2216 76-77 -1.291168238501477 2216 78-79 -1.1413465988363036 2216 80-81 -1.1889321812631906 2216 82-83 -1.1920371370478016 2216 84-85 -1.281144717346212 2216 86-87 -1.406796374670492 2216 88-89 -1.488690045529708 2216 90-91 -1.4163056968700083 2216 92-93 -1.4561555935952626 2216 94-95 -1.6845509963593024 2216 96-97 -1.596810161768886 2216 98-99 -1.4768215841362 2216 100-101 -1.6199518787923708 2216 102-103 -1.732938657883075 2216 104-105 -1.7086544692995496 2216 106-107 -1.6316726401103878 2216 108-109 -1.7043899409377197 2216 110-111 -1.7228757325045727 2216 112-113 -1.7733029165489178 2216 114-115 -1.789611409151199 2216 116-117 -1.9487321706822343 2216 118-119 -2.098573957718923 2216 120-121 -2.232657258805826 2216 122-123 -2.2393542941979874 2216 124-125 -2.6329173819292073 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57.0 3 12.0 4 49.0 5 194.0 6 558.0 7 997.0 8 1662.0 9 3391.0 10 6963.0 11 8998.0 12 8603.0 13 10492.0 14 15063.0 15 15186.0 16 8725.0 17 7603.0 18 7812.0 19 7647.0 20 7464.0 21 7364.0 22 7650.0 23 8366.0 24 9505.0 25 10765.0 26 12855.0 27 16088.0 28 19656.0 29 25000.0 30 31735.0 31 40420.0 32 52824.0 33 69714.0 34 97003.0 35 145398.0 36 235909.0 37 411216.0 38 208697.0 39 1437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.958491370013625 18.81888075836564 12.045025329707038 23.177602541913693 2 13.723674984045445 22.918882577100554 37.6341692732339 25.723273165620103 3 21.261043470039496 28.27489442390117 29.657560509286498 20.80650159677284 4 13.21646035199773 18.279431519593874 35.0846772128545 33.419430915553896 5 11.47610299669485 39.48681551437287 34.99019748167855 14.046884007253732 6 30.93623196018437 32.4490157712965 19.351551522632665 17.26320074588646 7 23.881442710091015 33.31037543710828 23.778493287934037 19.02968856486667 8 27.331627139253538 33.01039080073378 22.721620297844233 16.93636176216845 9 28.433212218940856 14.637201771675516 21.47683834971026 35.45274765967337 10-11 23.784163172368558 27.407782865798268 27.27134848446549 21.536705477367683 12-13 26.569049607614488 25.36208332020129 27.43914610188366 20.629720970300568 14-15 26.06222938908781 24.155999260698128 26.668049848380743 23.113721501833318 16-17 21.75540479985293 27.623693631437874 30.956244552523117 19.664657016186084 18-19 23.017665543064766 25.729936352570252 32.32116149008783 18.931236614277143 20-21 21.115546219177066 25.789020825967473 32.84553710274842 20.249895852107038 22-23 20.293346762280066 26.795869942314184 29.493433691511534 23.41734960389422 24-25 21.019639178032904 27.805470238556396 28.594924225811152 22.579966357599545 26-27 22.357686211737022 24.86198999657273 29.578032116542946 23.2022916751473 28-29 21.790912188713214 24.427062918192366 31.846470917677618 21.935553975416806 30-31 23.40645062170158 25.84982515668928 30.689334361076714 20.05438986053242 32-33 19.701092130540918 26.080771963090534 32.04789905704107 22.17023684932748 34-35 18.6196758272891 27.051816815564806 32.668998350050586 21.659509007095505 36-37 22.804150542519817 25.669138415760717 30.222089741956747 21.304621299762715 38-39 23.86168009780195 24.698406778904296 30.133092330136733 21.306820793157012 40-41 21.372786799354202 26.532145124639133 30.227394938010356 21.867673137996306 42-43 23.489341977232943 25.756494414599906 29.63318359269847 21.120980015468678 44-45 24.647227522162357 24.29212423789195 29.454466547346886 21.606181692598803 46-47 21.967817800532867 25.775108037802397 32.62836834357794 19.628705818086793 48-49 23.51498084799334 25.698847990713542 31.103791138733538 19.68238002255958 50-51 20.789677219420145 26.5035342904303 31.26077587621908 21.446012613930478 52-53 20.272927584798676 27.60498805707915 27.936192368348827 24.18589198977334 54-55 21.169237557104758 28.179318459067755 27.05150359994695 23.599940383880536 56-57 22.674774371371655 25.51258044565019 27.825626789960857 23.987018393017298 58-59 22.292587772917734 24.941697010921303 29.94803943067919 22.817675785481768 60-61 23.63864490196825 26.371269232435896 28.961944168322308 21.028141697273547 62-63 20.087382261446887 26.566334751076436 30.380978518500037 22.965304468976637 64-65 18.872441201304202 27.30231150341611 31.15730120190824 22.667946093371448 66-67 22.74272886877757 26.328034414521124 28.748002400402346 22.181234316298966 68-69 23.723473124816984 25.262100824777196 28.669805485996118 22.344620564409702 70-71 21.387578311813314 26.831751230074886 28.86972958705989 22.91094087105191 72-73 23.418925360355804 26.306564732732006 28.179712398183156 22.094797508729034 74-75 24.489093795590243 24.720697167183822 28.224785598636444 22.565423438589487 76-77 21.78903509866205 26.15154969082345 31.551141832525975 20.50827337798852 78-79 23.109551841731022 25.98140082123174 30.372574484038246 20.536472852998994 80-81 20.67625558244555 26.681003861916462 30.41528404979916 22.227456505838834 82-83 20.170043819160934 27.55893000883737 27.36872307262005 24.902303099381648 84-85 20.944922059146016 28.02223523680337 26.6301857160303 24.40265698802031 86-87 22.287630705715664 25.294469488758946 27.5706168692608 24.847282936264588 88-89 22.067385911949355 24.90634097531446 29.273845462937555 23.752427649798634 90-91 23.11345840462537 26.259817291038278 28.36867186053505 22.2580524438013 92-93 20.095162558975968 26.527203465613713 29.327815121746887 24.04981885366344 94-95 18.915068216131502 27.158027226048027 30.221392607727783 23.705511950092692 96-97 22.47301449732115 26.055126588927408 28.137442220821512 23.33441669292993 98-99 23.285557547945952 25.168267983876742 27.971815153737616 23.574359314439693 100-101 21.175048159056857 26.53583729789282 28.1916290564239 24.097485486626425 102-103 22.827100034700873 25.87604927458107 27.92914587596392 23.36770481475414 104-105 23.8291064840293 24.406163682763676 28.058068227639115 23.70666160556791 106-107 21.51698821922486 25.71919202532144 30.899252702223507 21.864567053230193 108-109 22.691029158551014 25.86118922127848 29.469955568047347 21.97782605212316 110-111 20.290735394936263 26.461655551382034 29.824806215803758 23.42280283787795 112-113 19.7320091499461 27.169016262594393 27.19987943662231 25.899095150837198 114-115 20.413002447064294 27.746641649750252 26.45988847482309 25.380467428362362 116-117 21.625763244458923 25.409537797021635 27.14634588504829 25.818353073471144 118-119 21.231445650799802 25.09774433621359 28.648686809316864 25.022123203669743 120-121 22.238193706155485 26.26399265063667 27.843298072762114 23.654515570445735 122-123 19.691143013784977 26.493153473163783 28.5787588597767 25.236944653274545 124-125 18.70623292692255 27.343745126960826 29.18567596501359 24.76434598110303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 697.0 1 646.0 2 1622.5 3 2198.5 4 1196.0 5 766.0 6 1299.0 7 2654.0 8 3907.0 9 4070.5 10 3207.0 11 2262.0 12 2042.5 13 2114.5 14 2375.5 15 2766.5 16 3041.5 17 3275.5 18 3564.5 19 3648.0 20 3814.5 21 4369.0 22 4981.0 23 5632.5 24 6430.0 25 7599.5 26 9348.0 27 11036.5 28 12254.0 29 13845.0 30 16373.5 31 18715.0 32 21037.0 33 23963.5 34 26987.5 35 29040.5 36 31497.0 37 33776.5 38 33740.0 39 33718.0 40 33974.0 41 34494.0 42 35332.0 43 37140.0 44 39551.5 45 41049.0 46 42768.5 47 48492.5 48 56493.0 49 81914.0 50 172040.5 51 171442.0 52 72725.5 53 38503.0 54 32083.0 55 28609.5 56 25606.5 57 22510.5 58 20479.0 59 18533.5 60 15766.5 61 12846.0 62 10636.5 63 8590.0 64 6787.0 65 5346.5 66 3773.0 67 2778.5 68 2065.5 69 1334.0 70 964.5 71 761.5 72 661.0 73 602.5 74 461.0 75 345.5 76 234.5 77 116.5 78 52.0 79 23.5 80 12.5 81 6.0 82 3.0 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1532423158892716 2 1.3131303846552835E-4 3 3.939391153965851E-4 4 0.0 5 0.0 6 0.0038080781155003227 7 0.0 8 0.0 9 0.0 10-11 9.848477884914627E-5 12-13 0.005646460654017719 14-15 0.0012803021250389014 16-17 6.893934519440239E-4 18-19 0.0 20-21 2.297978173146746E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.282825961638209E-4 30-31 0.0 32-33 0.0 34-35 6.565651923276418E-5 36-37 0.0 38-39 0.0 40-41 6.565651923276418E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.520195288750807E-4 96-97 0.002494947730845039 98-99 0.004005047673198614 100-101 0.0 102-103 0.004037875932814997 104-105 0.02311109476993299 106-107 0.016939381962053156 108-109 0.019696955769829255 110-111 0.016414129808191045 112-113 0.014936858125453851 114-115 0.04211865708781822 116-117 0.041363607116641436 118-119 0.023340892587247666 120-121 0.01477271682737194 122-123 0.0028232303270088596 124-125 0.0019368673173665433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1523078.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.167147961872985 #Duplication Level Percentage of deduplicated Percentage of total 1 86.53421660138292 46.873117144118744 2 9.027275507838903 9.779635362514039 3 1.9984566267953519 3.2475208739702817 4 0.7694586289946916 1.6671751762918157 5 0.38240154828879597 1.0356800623504263 6 0.22953046294171583 0.7459806328752675 7 0.15522076515144534 0.5885506306899435 8 0.11565517502372757 0.5011768782453254 9 0.09625734832616017 0.46925874262805983 >10 0.5898692586623623 6.271397612696135 >50 0.06106536753207607 2.2756457002148074 >100 0.03257650191809692 3.5241635169251695 >500 0.0030700367784497777 1.0820270210162708 >1k 0.0022172487844359504 3.702745984913859 >5k 0.0015350183892248888 5.361539970770002 >10k+ 0.0011939031916193578 12.874384689779832 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 47490 3.118028098363971 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 25451 1.671024070993081 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15122 0.99285788383786 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13637 0.8953579527772051 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 13108 0.8606256541030729 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 12528 0.8225448729480697 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12018 0.7890600481393599 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8599 0.5645804088825391 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7566 0.49675722451509374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6807 0.4469239264174258 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6797 0.44626736122509814 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6403 0.42039869264738905 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 5740 0.3768684203960664 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5511 0.3618330774917634 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5315 0.3489643997221416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5296 0.3477169258567191 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4840 0.3177775530865786 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4641 0.30471190575925855 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4514 0.2963735278166975 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4013 0.2634796116810827 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3893 0.25560082937315093 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 3612 0.23715134746874422 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2776 0.18226249739015335 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2578 0.16926250658206604 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2211 0.1451665640236416 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1973 0.12954031244624373 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1801 0.1182473911382083 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1684 0.11056557838797487 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1543 0.10130800917615512 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.3131303846552835E-4 0.0 0.0 0.0 0.0 9 1.3131303846552835E-4 0.0 0.0 0.0 0.0 10-11 1.3131303846552835E-4 0.0 0.0 6.565651923276418E-5 0.0 12-13 1.3131303846552835E-4 0.0 0.0 3.282825961638209E-4 0.0 14-15 1.3131303846552835E-4 0.0 0.0 7.22221711560406E-4 0.0 16-17 1.3131303846552835E-4 0.0 0.0 0.0019368673173665433 0.0 18-19 1.6414129808191045E-4 0.0 0.0 0.0029217151058580056 0.0 20-21 1.9696955769829254E-4 0.0 0.0 0.0043661585289788175 0.0 22-23 1.9696955769829254E-4 0.0 0.0 0.007025247557905767 0.0 24-25 1.9696955769829254E-4 0.0 0.0 0.010833325673406089 0.0 26-27 1.9696955769829254E-4 0.0 0.0 0.01398483859657877 0.0 28-29 2.297978173146746E-4 0.0 0.0 0.018941905798652466 0.0 30-31 2.626260769310567E-4 0.0 0.0 0.029939372770140464 0.0 32-33 2.626260769310567E-4 0.0 0.0 0.04888127856879293 0.0 34-35 3.282825961638209E-4 0.0 0.0 0.07951004479087742 0.0 36-37 3.939391153965851E-4 0.0 0.0 0.12461607350378641 0.0 38-39 4.5959563462934927E-4 0.0 0.0 0.1917170361596714 0.0 40-41 4.5959563462934927E-4 0.0 0.0 0.2907270671626798 0.0 42-43 4.5959563462934927E-4 0.0 0.0 0.37181286841514355 0.0 44-45 4.5959563462934927E-4 0.0 0.0 0.4579213933889138 0.0 46-47 5.252521538621134E-4 0.0 0.0 0.5695374760846128 0.0 48-49 5.252521538621134E-4 0.0 0.0 0.6769515415494151 0.0 50-51 5.252521538621134E-4 0.0 0.0 0.7887974220624288 0.0 52-53 5.252521538621134E-4 0.0 0.0 0.9184362192875217 0.0 54-55 5.252521538621134E-4 0.0 0.0 1.0531962250127702 0.0 56-57 5.252521538621134E-4 0.0 0.0 1.2103122755367748 0.0 58-59 5.252521538621134E-4 0.0 0.0 1.3954308315135535 0.0 60-61 5.252521538621134E-4 0.0 0.0 1.5657110141437274 0.0 62-63 5.252521538621134E-4 0.0 0.0 1.7359911967739012 0.0 64-65 5.252521538621134E-4 0.0 0.0 1.938738528164677 0.0 66-67 5.252521538621134E-4 0.0 0.0 2.166238367306205 0.0 68-69 5.252521538621134E-4 0.0 0.0 2.441470495929952 0.0 70-71 5.252521538621134E-4 0.0 0.0 2.8190283097779627 0.0 72-73 5.252521538621134E-4 0.0 0.0 3.132899299970192 0.0 74-75 5.252521538621134E-4 0.0 0.0 3.4356415101524673 0.0 76-77 5.252521538621134E-4 0.0 0.0 3.7914013596152003 0.0 78-79 5.252521538621134E-4 0.0 0.0 4.158486958645585 0.0 80-81 7.878782307931702E-4 0.0 0.0 4.5410346679552855 0.0 82-83 7.878782307931702E-4 0.0 0.0 4.988812129122737 0.0 84-85 7.878782307931702E-4 0.0 0.0 5.429728484030365 0.0 86-87 7.878782307931702E-4 0.0 0.0 5.893361994592529 0.0 88-89 7.878782307931702E-4 0.0 0.0 6.397144466665528 0.0 90-91 7.878782307931702E-4 0.0 0.0 6.901583503930856 0.0 92-93 7.878782307931702E-4 0.0 0.0 7.421780105812046 0.0 94-95 7.878782307931702E-4 0.0 0.0 7.985671121242641 0.0 96-97 7.878782307931702E-4 0.0 0.0 8.561741420990915 0.0 98-99 8.207064904095522E-4 0.0 0.0 9.212692980924155 0.0 100-101 8.863630096423165E-4 0.0 0.0 10.073285806767611 0.0 102-103 9.191912692586985E-4 0.0 0.0 10.804830744059071 0.0 104-105 0.0010505043077242268 0.0 0.0 11.490120663551046 0.0 106-107 0.001116160826956991 0.0 0.0 12.242314576141208 0.0 108-109 0.001116160826956991 0.0 0.0 12.951273670816597 0.0 110-111 0.0011818173461897552 0.0 0.0 13.6967377901854 0.0 112-113 0.0011818173461897552 0.0 0.0 14.565209398336789 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 75 1.4716552E-6 47.597565 2 AGTACTC 1310 0.0 42.238304 5 AAAGTAC 1725 0.0 31.386803 3 AAGTACT 1775 0.0 30.837864 4 GTTATCA 785 0.0 30.358784 1 ATCACGC 140 3.0965075E-6 29.748482 3 ATAACGC 220 1.5825208E-10 29.748482 3 TAACGCA 200 6.121627E-8 26.773632 4 GACGGGA 90 0.009532285 26.443096 4 TATAACG 215 1.2331839E-7 24.905704 2 AGCGTCA 120 0.0012884379 24.790401 3 TCACGCA 180 2.1785112E-5 23.137709 4 AAAAAGT 2385 0.0 22.732494 1 CATGGGG 4010 0.0 22.107351 4 ATAGCGC 135 0.002555444 22.037357 6 CATGGGT 3555 0.0 20.92017 4 TTATCAA 1120 0.0 20.717693 2 TTAGGGC 490 0.0 20.641804 3 GAGTACT 10350 0.0 19.804228 12-13 AAAAGTA 2750 0.0 19.688086 2 >>END_MODULE