FastQCFastQC Report
Thu 26 May 2016
SRR1364147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301750
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA698635.366852314192434No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT499693.838601882081813No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG336272.583214903015172No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT225071.7289802189360475No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC173931.3361244478586518No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC172601.3259074323026694No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG161051.2371807182638754No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC151391.1629729210677933No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG136601.0493566352986365No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT112680.8656039946226234No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC75670.5812944113693106No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT72100.5538697906664106No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70580.5421932014595737No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA67220.5163817937391972No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG64550.4958709429613981No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG56790.4362588822738621No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA52750.4052237372767428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50870.3907816400998656No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49010.3764931822546572No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC48950.3760322642596505No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA46430.3566737084693682No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC45020.3458421355867102No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA43520.3343191857115422No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT42960.33001728442481276No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40990.3148838102554254No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC34630.2660265027847129No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG23920.18375264067601305No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC21810.16754369118494333No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT13690.10516612252736701No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC12250.046.175115
ATAACGC2250.042.3341453
TATAACG2050.040.6515582
AAGTACT14500.039.0039834
TACGGGT800.005348202329.7661954
AAAGTAC19000.027.8862273
AAAAAGT20150.026.5901531
AAAAGTA20300.026.0973852
GGTGCGC1150.001001469625.884658
GTACACG2651.369699E-924.711561
CATGGGG57850.023.8747024
GTATAAC4650.023.0447961
GTGCGCT1300.002045356722.8979579
ATGGGGG18700.022.60675
ATGGGAG19650.022.4227895
TGGGGGG10300.021.3862486
ATGGGGA34250.021.0351335
GTACTAG1754.7663393E-420.4111061
GTATAGG2951.1514021E-720.1804711
TATTGGG2951.1528027E-720.1781332
Produced by FastQC (version 0.11.5)