##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364147_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1301750 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.289961974265413 31.0 31.0 34.0 26.0 34.0 2 30.370029575571348 31.0 31.0 34.0 26.0 34.0 3 30.501315152679087 31.0 31.0 34.0 26.0 34.0 4 33.8541263683503 37.0 35.0 37.0 28.0 37.0 5 33.8917380449395 37.0 35.0 37.0 30.0 37.0 6 33.807297868254274 37.0 35.0 37.0 28.0 37.0 7 33.71559669675437 37.0 35.0 37.0 28.0 37.0 8 33.61591165738429 37.0 35.0 37.0 28.0 37.0 9 34.957230651046665 39.0 35.0 39.0 27.0 39.0 10-11 34.892577683887076 39.0 35.0 39.0 27.0 39.0 12-13 34.762106011138854 39.0 35.0 39.0 26.5 39.0 14-15 35.90093489533321 40.0 36.0 41.0 26.0 41.0 16-17 35.926267716535435 40.0 36.0 41.0 27.0 41.0 18-19 35.864275782600345 40.0 36.0 41.0 26.0 41.0 20-21 35.89066909928942 40.0 36.0 41.0 27.0 41.0 22-23 35.90507163433839 40.0 36.0 41.0 27.0 41.0 24-25 35.93598732475514 40.0 36.0 41.0 27.0 41.0 26-27 35.75570309199155 40.0 36.0 41.0 26.0 41.0 28-29 35.63255924716727 40.0 35.5 41.0 25.0 41.0 30-31 35.67832763587478 40.0 35.0 41.0 26.0 41.0 32-33 35.326 39.0 35.0 41.0 25.5 41.0 34-35 35.3745965047052 39.0 35.0 41.0 25.5 41.0 36-37 35.36544689840599 39.5 35.0 41.0 25.5 41.0 38-39 35.18953293643173 39.0 34.5 41.0 25.0 41.0 40-41 34.79696408680622 39.0 34.0 41.0 24.0 41.0 42-43 34.52249855963126 39.0 33.0 41.0 23.5 41.0 44-45 34.23791818705588 38.0 33.0 40.5 22.5 41.0 46-47 33.937829844440174 38.0 33.0 40.0 21.0 41.0 48-49 33.606539658152485 38.0 32.5 40.0 17.0 41.0 50-51 33.452526214710964 38.0 32.5 40.0 15.5 41.0 52-53 33.26202803917803 38.0 32.0 40.0 13.5 41.0 54-55 32.91701632417899 38.0 32.0 40.0 9.0 41.0 56-57 32.44613059343192 37.0 31.0 40.0 7.5 41.0 58-59 32.02801920491646 37.0 31.0 40.0 4.0 41.0 60-61 31.71458843864029 36.0 30.0 40.0 2.0 41.0 62-63 31.101334357595547 36.0 28.5 39.0 2.0 41.0 64-65 30.80688688304206 35.5 29.0 39.0 2.0 41.0 66-67 30.3399631265604 35.0 28.5 39.0 2.0 41.0 68-69 29.82540234299981 35.0 28.0 38.0 2.0 40.0 70-71 29.121395429229885 34.0 26.5 37.5 2.0 40.0 72-73 28.793483387747266 34.0 26.5 37.0 2.0 39.5 74-75 28.33674822354523 34.0 26.0 36.5 2.0 39.0 76-77 27.74485884386403 34.0 26.0 36.0 2.0 39.0 78-79 27.297858651814863 34.0 25.5 35.5 2.0 37.5 80-81 27.017019396965622 34.0 25.0 35.0 2.0 37.0 82-83 26.76668100633762 34.0 24.0 35.0 2.0 37.0 84-85 26.458126752448628 33.5 23.5 35.0 2.0 36.0 86-87 26.01617361244479 33.0 20.5 35.0 2.0 36.0 88-89 25.6829756097561 33.0 20.0 35.0 2.0 36.0 90-91 25.493031688112154 33.0 19.0 35.0 2.0 35.5 92-93 25.10664989437296 33.0 17.5 35.0 2.0 35.0 94-95 24.992370270789323 33.0 17.0 35.0 2.0 35.0 96-97 24.856033416554638 33.0 13.5 35.0 2.0 35.0 98-99 24.586926445169965 32.0 7.0 35.0 2.0 35.0 100-101 24.035941617053965 32.0 4.5 35.0 2.0 35.0 102-103 22.84781870558863 30.0 2.0 34.0 2.0 35.0 104-105 23.462901862876897 31.5 2.0 35.0 2.0 35.0 106-107 23.47153293643173 32.0 2.0 35.0 2.0 35.0 108-109 23.387041674668716 32.0 2.0 35.0 2.0 35.0 110-111 23.432286537353562 32.0 2.0 35.0 2.0 35.0 112-113 23.316493950451317 32.0 2.0 35.0 2.0 35.0 114-115 23.149674668715193 32.0 2.0 35.0 2.0 35.0 116-117 22.789759938544265 32.0 2.0 35.0 2.0 35.0 118-119 22.5593693105435 32.0 2.0 35.0 2.0 35.0 120-121 22.34913769925101 32.0 2.0 35.0 2.0 35.0 122-123 21.947468792010753 31.5 2.0 35.0 2.0 35.0 124-125 21.0143107355483 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6934858490957474 1101 2 0.7348939133130585 1101 3 0.7261767824482632 1101 4 0.820437945404116 1101 5 0.6652368229194892 1101 6 0.749383638033116 1101 7 0.767758826802428 1101 8 0.6970172559329981 1101 9 0.8266763744304058 1101 10-11 0.8025479688887103 1101 12-13 0.8389871412086762 1101 14-15 1.0476050003073993 1101 16-17 0.9082193765833253 1101 18-19 0.9287576514911322 1101 20-21 0.9598775970096156 1101 22-23 0.928326181577269 1101 24-25 0.9515113861402256 1101 26-27 1.0063350154532955 1101 28-29 1.128018629098392 1101 30-31 1.1030490757834315 1101 32-33 1.286711917268164 1101 34-35 1.223346292083427 1101 36-37 1.1286077303184356 1101 38-39 1.1066240229715518 1101 40-41 1.1667599083897429 1101 42-43 1.161922992566133 1101 44-45 1.1558186432924842 1101 46-47 1.1663007206944442 1101 48-49 1.1344806269443026 1101 50-51 0.9732205533413563 1101 52-53 1.3864360822084976 1101 54-55 1.3293805826177731 1101 56-57 1.4309944566711863 1101 58-59 1.2999820118666214 1101 60-61 1.345585591903788 1101 62-63 1.3535851975141924 1101 64-65 1.5689892600043507 1101 66-67 1.6017877600411303 1101 68-69 1.5205639781911273 1101 70-71 1.54751977108792 1101 72-73 1.346477042624585 1101 74-75 1.5687533110149872 1101 76-77 1.3636066091831474 1101 78-79 1.3189890141671832 1101 80-81 1.3330131374862866 1101 82-83 1.4047050809555763 1101 84-85 1.4825506090090954 1101 86-87 1.2557442367680274 1101 88-89 1.199689697891369 1101 90-91 1.1484805976034984 1101 92-93 1.001848324149389 1101 94-95 0.9975071001385736 1101 96-97 1.0443560542967276 1101 98-99 0.9641453724409956 1101 100-101 0.961119042185711 1101 102-103 0.759445175037051 1101 104-105 0.8622561156416815 1101 106-107 0.6590572324265196 1101 108-109 0.7067208089529373 1101 110-111 0.8821794939406402 1101 112-113 1.0233070743262864 1101 114-115 1.1474805274020454 1101 116-117 1.0413591314933335 1101 118-119 0.7976348059817226 1101 120-121 0.6895045200833003 1101 122-123 0.6557468359490208 1101 124-125 0.6285297257084892 1102 1 0.4265663120821337 1102 2 0.5117904762454124 1102 3 0.5149054447462262 1102 4 0.6851589047663253 1102 5 0.505041479634329 1102 6 0.54262703469238 1102 7 0.6276188153931201 1102 8 0.7724876074972045 1102 9 0.9257091736175269 1102 10-11 0.8041594991735579 1102 12-13 0.8878260244525791 1102 14-15 1.0115104433792936 1102 16-17 0.892997928957108 1102 18-19 0.9434348955623619 1102 20-21 0.8205667864725328 1102 22-23 0.868539515023663 1102 24-25 0.9997290458929982 1102 26-27 1.0417999873987256 1102 28-29 1.1966670634738819 1102 30-31 1.0002844604850623 1102 32-33 0.9991963778483282 1102 34-35 1.0191874039592435 1102 36-37 0.9538341854181596 1102 38-39 1.0171718443437072 1102 40-41 1.0051865677453975 1102 42-43 1.1730297619825762 1102 44-45 1.2583043851828961 1102 46-47 1.313364164865824 1102 48-49 1.268132474221538 1102 50-51 1.307317608824448 1102 52-53 1.241311915236814 1102 54-55 1.3030505990628285 1102 56-57 1.2036955528980613 1102 58-59 1.2094749898624748 1102 60-61 1.223725803046758 1102 62-63 1.1975774305129292 1102 64-65 1.302421258937855 1102 66-67 1.348170884996673 1102 68-69 1.3568596348791218 1102 70-71 1.3078795413376838 1102 72-73 1.2009317391212875 1102 74-75 1.3692192698519534 1102 76-77 1.2067413771128948 1102 78-79 1.0867516486164348 1102 80-81 1.15036188988109 1102 82-83 1.2709638481561036 1102 84-85 1.103590736160296 1102 86-87 1.0916970723029031 1102 88-89 1.0533131914685647 1102 90-91 0.9309433230163542 1102 92-93 0.9382843832347518 1102 94-95 0.835326550333992 1102 96-97 0.6763750553740628 1102 98-99 0.7179880077570147 1102 100-101 0.7630766440063042 1102 102-103 0.5698494142897061 1102 104-105 0.8795481512149976 1102 106-107 1.0112511464854634 1102 108-109 0.7580516522105363 1102 110-111 0.8544663312571075 1102 112-113 0.9038702337358835 1102 114-115 0.8672119280296045 1102 116-117 0.8436559036749607 1102 118-119 0.8531410954721039 1102 120-121 0.6047272395983612 1102 122-123 0.5977372807662427 1102 124-125 0.5701676924387016 1103 1 0.2968146297305765 1103 2 0.17704445739003205 1103 3 0.2959311137530811 1103 4 0.3117322144962813 1103 5 0.2928791923380629 1103 6 0.15566795065053896 1103 7 0.2642526134810481 1103 8 0.32772373215797046 1103 9 0.368786339120895 1103 10-11 0.3255690542828944 1103 12-13 0.3193358697439237 1103 14-15 0.29090860719753664 1103 16-17 0.2929598478035018 1103 18-19 0.3001887421098033 1103 20-21 0.22274067244596552 1103 22-23 0.33152925235652475 1103 24-25 0.33855124245106083 1103 26-27 0.5547359198198478 1103 28-29 0.5420403731132737 1103 30-31 0.432494912969311 1103 32-33 0.3776041826282963 1103 34-35 0.30439045325893943 1103 36-37 0.34034997041857906 1103 38-39 0.4983748782479367 1103 40-41 0.5806747570408888 1103 42-43 0.6270141668219154 1103 44-45 0.639894411452552 1103 46-47 0.6605582155119478 1103 48-49 0.5833897495444305 1103 50-51 0.5864017502880117 1103 52-53 0.7014789606396548 1103 54-55 0.8071520208120546 1103 56-57 0.7392463021481532 1103 58-59 0.5376348030157736 1103 60-61 0.5801554390593004 1103 62-63 0.5805301824875677 1103 64-65 0.6370721881112082 1103 66-67 0.6578770737275015 1103 68-69 0.6435036324674641 1103 70-71 0.5413210882385187 1103 72-73 0.6735133085221534 1103 74-75 0.5950480952928778 1103 76-77 0.5680085874963225 1103 78-79 0.38099070815231073 1103 80-81 0.5108561569920802 1103 82-83 0.5103680841297553 1103 84-85 0.5819041757510668 1103 86-87 0.4594217777116718 1103 88-89 0.4042760763808424 1103 90-91 0.507360561145596 1103 92-93 0.5386544858614215 1103 94-95 0.7712097285843562 1103 96-97 0.6595808591048851 1103 98-99 0.628294891593427 1103 100-101 0.6060418074484843 1103 102-103 0.622238961397926 1103 104-105 0.7048435008993792 1103 106-107 0.626811712613204 1103 108-109 0.49059086595018186 1103 110-111 0.5580508500229406 1103 112-113 0.5218077023081804 1103 114-115 0.44695628621160566 1103 116-117 0.49647113559303335 1103 118-119 0.39443877077842515 1103 120-121 0.3187591711663913 1103 122-123 0.40475028065779384 1103 124-125 0.4082333600146839 1104 1 0.30003428439307456 1104 2 0.38004423770029305 1104 3 0.43556355805065294 1104 4 0.492349256512405 1104 5 0.35144450757437795 1104 6 0.4540596925317075 1104 7 0.47395454619690724 1104 8 0.42225031923172196 1104 9 0.6137950897878071 1104 10-11 0.5479001470270433 1104 12-13 0.4574004862191856 1104 14-15 0.5423425904309624 1104 16-17 0.5127315089348556 1104 18-19 0.4318711639768722 1104 20-21 0.3845355883823274 1104 22-23 0.453331751281155 1104 24-25 0.45046562622134445 1104 26-27 0.6128978551572217 1104 28-29 0.6094772769279402 1104 30-31 0.47549133331885685 1104 32-33 0.5641244633142506 1104 34-35 0.4986538741789488 1104 36-37 0.5826557526894334 1104 38-39 0.5705918800183767 1104 40-41 0.5241786099189127 1104 42-43 0.7056432474665115 1104 44-45 0.6213270516247604 1104 46-47 0.5359821336322952 1104 48-49 0.6309126554304569 1104 50-51 0.6868830369683465 1104 52-53 0.9087482679523617 1104 54-55 0.7945819370161118 1104 56-57 0.882891661063951 1104 58-59 0.8710139819669145 1104 60-61 0.8810588438810321 1104 62-63 0.822231125676538 1104 64-65 0.9870834033646929 1104 66-67 0.8928643261842417 1104 68-69 0.9364780146131508 1104 70-71 0.586338446497102 1104 72-73 0.7285263191392595 1104 74-75 0.6263834212182964 1104 76-77 0.511713438024227 1104 78-79 0.7326345966237682 1104 80-81 0.7136479012732266 1104 82-83 0.7338925859971148 1104 84-85 0.6116023285111254 1104 86-87 0.5681549294471289 1104 88-89 0.6189229897400104 1104 90-91 0.5513461622370315 1104 92-93 0.6579255913069559 1104 94-95 0.782158100572623 1104 96-97 0.5656923414937047 1104 98-99 0.3926503006686417 1104 100-101 0.363054802373469 1104 102-103 0.37872638554496874 1104 104-105 0.4136495979357875 1104 106-107 0.3773788213670102 1104 108-109 0.47090578842185593 1104 110-111 0.3947641438367384 1104 112-113 0.4978662514697625 1104 114-115 0.5561366634871945 1104 116-117 0.49356266959971506 1104 118-119 0.4901424979348441 1104 120-121 0.4833729002981748 1104 122-123 0.4964984386793283 1104 124-125 0.488325154326013 1105 1 0.27964052123667926 1105 2 0.388034874179894 1105 3 0.289088783932808 1105 4 0.22419637994224217 1105 5 0.17709168918225515 1105 6 0.027785131471297575 1105 7 0.20725317252679787 1105 8 0.12429912982484126 1105 9 0.1994939535470195 1105 10-11 0.22987212722291872 1105 12-13 0.25808540493698473 1105 14-15 0.1599634621975099 1105 16-17 0.1663881267856837 1105 18-19 0.22337522857808523 1105 20-21 0.22673145086201174 1105 22-23 0.29286869532213444 1105 24-25 0.46561058979128234 1105 26-27 0.44335946649250246 1105 28-29 0.5368977096074872 1105 30-31 0.43419376637971396 1105 32-33 0.33064968133450634 1105 34-35 0.5653104841678811 1105 36-37 0.5658745316187606 1105 38-39 0.5896048973558266 1105 40-41 0.6693270572923495 1105 42-43 0.710413945386847 1105 44-45 0.6492428084405333 1105 46-47 0.6690437728773091 1105 48-49 0.6197101879738014 1105 50-51 0.5043110956888697 1105 52-53 0.567777141755144 1105 54-55 0.6720938133956338 1105 56-57 0.5957511954823644 1105 58-59 0.8195893077934393 1105 60-61 0.7322237806025846 1105 62-63 0.6110858535271149 1105 64-65 0.8036685192195847 1105 66-67 0.7522547735851965 1105 68-69 0.8567088645039647 1105 70-71 0.7688282063750833 1105 72-73 0.6674335988636209 1105 74-75 0.56920285504372 1105 76-77 0.4188338361114248 1105 78-79 0.3935755085646129 1105 80-81 0.33167525772970663 1105 82-83 0.41954184609425127 1105 84-85 0.3166459079939301 1105 86-87 0.4159806478771877 1105 88-89 0.3492980956299476 1105 90-91 0.3835011526749952 1105 92-93 0.21529668566349258 1105 94-95 0.25461185295821664 1105 96-97 0.24548043386314333 1105 98-99 0.18360476946968163 1105 100-101 0.2758270451567384 1105 102-103 -0.009436519858709858 1105 104-105 -0.08629217488288177 1105 106-107 -0.20022413018600815 1105 108-109 -0.1960728154230047 1105 110-111 -0.09692583008141753 1105 112-113 -0.07159939007093641 1105 114-115 -0.07145297197625311 1105 116-117 -0.21607471510064258 1105 118-119 -0.2667555804207602 1105 120-121 -0.2973090161573637 1105 122-123 -0.35935366329734464 1105 124-125 -0.2646735455126219 1106 1 0.2817797796128936 1106 2 0.31033383541951665 1106 3 0.2974046474457559 1106 4 0.23956066152928912 1106 5 0.21913842167237618 1106 6 0.3025905193700069 1106 7 0.29739555047713395 1106 8 0.2864892660669369 1106 9 0.3472567702040479 1106 10-11 0.2803762743754703 1106 12-13 0.2839551508722167 1106 14-15 0.3717607021988485 1106 16-17 0.3658205481347707 1106 18-19 0.3656271450858881 1106 20-21 0.211204296613424 1106 22-23 0.3158978235873704 1106 24-25 0.48103919127302674 1106 26-27 0.5692920161260204 1106 28-29 0.6291134861523631 1106 30-31 0.4878857164902186 1106 32-33 0.14893899008871614 1106 34-35 0.3898664222208339 1106 36-37 0.4769287643061091 1106 38-39 0.23452934694692118 1106 40-41 0.3073165666346114 1106 42-43 0.48335642539615264 1106 44-45 0.5339083532797915 1106 46-47 0.4255258086742302 1106 48-49 0.31272495264708766 1106 50-51 0.37416683670735296 1106 52-53 0.3055844943127468 1106 54-55 0.26496238356791935 1106 56-57 0.30206418722454487 1106 58-59 0.44911441375543504 1106 60-61 0.45842346509158816 1106 62-63 0.5288491368883541 1106 64-65 0.3594970032908762 1106 66-67 0.4338338637204693 1106 68-69 0.5415163869229431 1106 70-71 0.4919705044882008 1106 72-73 0.5338845313125695 1106 74-75 0.4918416574949376 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2216 108-109 -3.950239810792798 2216 110-111 -3.906638630711818 2216 112-113 -4.00054012689186 2216 114-115 -4.157594316388284 2216 116-117 -4.074129921270629 2216 118-119 -4.166839728420786 2216 120-121 -4.0156857811454145 2216 122-123 -4.000801930743801 2216 124-125 -4.043742646443835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41477.0 3 7117.0 4 4566.0 5 3155.0 6 3403.0 7 3918.0 8 5226.0 9 6276.0 10 8110.0 11 9786.0 12 11521.0 13 13044.0 14 13729.0 15 14183.0 16 14568.0 17 15085.0 18 15950.0 19 16498.0 20 17618.0 21 18108.0 22 18611.0 23 20359.0 24 22485.0 25 24927.0 26 28079.0 27 29579.0 28 32986.0 29 38822.0 30 43243.0 31 49150.0 32 57800.0 33 65796.0 34 73842.0 35 87406.0 36 130089.0 37 225550.0 38 108957.0 39 731.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.87470916004959 17.08255299964644 9.986521392079272 23.0562164482247 2 12.676821709375583 23.075397101073396 37.00407714621378 27.243704043337242 3 22.570773883991105 31.02906435154135 26.841710844470718 19.55845091999682 4 13.45752369956294 18.55971178491301 33.03776571564846 34.944998799875584 5 11.387888545350421 41.942599404476155 32.791398400513714 13.878113649659712 6 31.117862317078725 28.684186354896795 21.58376499628349 18.614186331740985 7 25.73907003665831 32.835423499903534 22.155431217441638 19.270075245996527 8 29.680595825883966 32.71507443191818 22.155190781002492 15.449138961195358 9 31.773416509681603 15.465164757110653 19.48156326240697 33.279855470800776 10-11 23.918215639214893 30.0957043638117 24.624484581950167 21.361595415023242 12-13 27.08494988028867 26.96299950818368 24.104558450091453 21.847492161436197 14-15 28.160067802575945 25.252522913472987 23.063605053082686 23.52380423086839 16-17 23.515006819702805 28.37691073252176 29.671843774387096 18.43623867338834 18-19 25.691710739320783 27.447001869671006 28.421546173541746 18.43974121746647 20-21 21.746854222080884 28.568842789063453 29.30810638912088 20.376196599734783 22-23 20.58993370039496 30.626742796441377 22.81909193936381 25.96423156379986 24-25 21.283651307765698 32.231422502030114 21.749767655840408 24.73515853436378 26-27 24.140140517247367 26.643079861381842 22.8889024415663 26.32787717980449 28-29 23.630103147536023 27.075070285759278 26.01684696771564 23.27797959898906 30-31 26.494231530900663 28.868055196331778 24.675984438297107 19.961728834470456 32-33 19.906306189458213 29.208906470632286 27.597019338631018 23.287768001278486 34-35 17.730224888505294 30.824686398225037 28.181584479288656 23.26350423398101 36-37 24.566350211703046 27.943683208595598 25.307021503658795 22.18294507604256 38-39 27.30487099386787 26.384048748505535 24.392224921902116 21.91885533572448 40-41 22.35659391852702 30.49359520195476 24.535143089852284 22.614667789665937 42-43 25.505543224422972 28.477421171279754 23.842903727816015 22.174131876481262 44-45 27.39261410679521 26.17664012000398 24.12788387269022 22.30286190051059 46-47 23.24328203640225 27.73070886072042 30.047752293082397 18.97825680979493 48-49 25.34009471490228 27.24538907267521 28.274721060214265 19.139795152208254 50-51 20.763249345711323 28.759922349513605 29.307426176485112 21.16940212828996 52-53 19.90386989260585 30.679352857671894 23.23841192445377 26.178365325268487 54-55 20.840085809326922 31.83968114297221 22.025885530728218 25.29434751697265 56-57 23.697682536192897 26.660264087121366 23.120216144914288 26.52183723177145 58-59 23.299903610386934 26.885836358285474 26.029245511171588 23.785014520155997 60-61 25.688514004047335 28.70337866977945 24.921248209134514 20.6868591170387 62-63 19.548413626670698 29.06052011936761 27.43317975194497 23.95788650201672 64-65 17.481077836086875 30.300385735130604 28.23744740413915 23.981089024643374 66-67 23.762375855677224 27.84343300883983 25.42151687051185 22.972674264971097 68-69 26.29317727662004 26.25147556920323 24.681701398801028 22.7736457553757 70-71 21.794047747650428 29.719980246608745 24.784217349033163 23.701754656707667 72-73 24.74292989698677 28.03935337011459 24.235772985068866 22.981943747829778 74-75 26.428741195281102 25.753069690777654 24.453582694907617 23.364606419033628 76-77 22.506666584363156 27.28241780965667 30.063054777101517 20.147860828878656 78-79 24.50473269356364 26.929387062466464 28.308748221165324 20.257132022804576 80-81 20.232794279792937 28.234764902545223 29.319070366046645 22.213370451615194 82-83 19.518406297283057 29.908005163377062 23.704545577295953 26.86904296204393 84-85 20.333746246839976 31.03637175177816 22.715476618049347 25.914405383332518 86-87 22.842763364757126 26.035760237810017 23.85048524586369 27.270991151569163 88-89 22.66191310345519 26.047592467078474 26.4342759579231 24.856218471543233 90-91 24.682396764482135 27.895617206511155 25.500727559458504 21.921258469548203 92-93 19.30201845650984 28.174399704313117 27.57835760288372 24.945224236293328 94-95 17.509772361462407 29.15439173704979 28.501422065706688 24.83441383578112 96-97 23.263092491980807 26.90886174663236 26.05242666249107 23.775619098895767 98-99 25.341374799165777 25.713682020483837 25.25253927328376 23.692403907066627 100-101 21.29239199670565 28.236898561498375 25.4970056278465 24.97370381394947 102-103 23.715802503688302 27.02769801076556 25.21394457159621 24.042554913949928 104-105 25.01876485430493 25.048989088049282 25.412913535175186 24.5193325224706 106-107 21.58102809471609 26.603629027749232 30.253786711815838 21.56155616571884 108-109 23.1167209605074 26.55481672743875 28.78784373245476 21.540618579599087 110-111 19.341033997338837 28.01893669128565 29.73087529166747 22.909154019708044 112-113 18.78295492059966 29.319196463005643 24.463156822999103 27.434691793395594 114-115 19.339836931831766 30.876821134844 23.650052101290154 26.13328983203408 116-117 21.51686242335262 26.367083883384947 24.594516859338952 27.52153683392348 118-119 21.24797581277122 26.443245011827777 26.721200595266343 25.58757858013466 120-121 22.952736308809513 28.03062165509737 26.04129447490575 22.975347561187363 122-123 18.714110452162505 28.362293089730183 27.43003743755426 25.493559020553054 124-125 17.34444266616275 29.317917559289093 28.071260675314257 25.266379099233905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5601.0 1 3132.5 2 962.0 3 1264.5 4 1180.0 5 1004.0 6 814.5 7 683.5 8 648.5 9 649.5 10 611.5 11 596.5 12 629.5 13 638.5 14 677.0 15 758.0 16 828.0 17 921.0 18 1088.5 19 1288.0 20 1512.0 21 1916.0 22 2430.0 23 3115.5 24 3852.5 25 4609.0 26 5772.0 27 7251.5 28 8382.0 29 9511.5 30 11193.5 31 12805.0 32 14436.0 33 16200.0 34 18351.5 35 20148.5 36 21777.0 37 23457.0 38 24017.0 39 24396.5 40 24722.5 41 25316.0 42 26277.5 43 27397.5 44 28934.5 45 30620.0 46 33674.5 47 40449.0 48 52037.0 49 83155.0 50 174660.5 51 188715.0 52 96291.0 53 46173.0 54 30529.0 55 23565.0 56 19582.5 57 16369.5 58 14144.5 59 12432.5 60 10546.0 61 8713.5 62 7205.5 63 5658.0 64 4319.0 65 3399.5 66 2442.0 67 1943.0 68 1532.0 69 1005.0 70 772.5 71 653.0 72 549.0 73 478.5 74 354.0 75 268.0 76 192.0 77 104.5 78 56.0 79 45.0 80 35.5 81 23.5 82 19.5 83 16.0 84 10.5 85 8.5 86 7.0 87 5.0 88 5.5 89 4.5 90 3.5 91 3.0 92 1.0 93 1.5 94 1.5 95 1.5 96 2.5 97 2.0 98 2.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4888803533704628 2 0.47904743614365275 3 0.46391396197426543 4 0.4648357979642788 5 0.46737084693681585 6 0.47466871519108894 7 0.4609179950067217 8 0.4868830420587671 9 0.5000960245822931 10-11 0.5042058767044363 12-13 0.5032456308815056 14-15 0.47812560015363936 16-17 0.47927789514115615 18-19 0.46610332245054736 20-21 0.4765892068369503 22-23 0.4930286153255233 24-25 0.4807374687920108 26-27 0.491108123679662 28-29 0.4858075667370847 30-31 0.5007874015748032 32-33 0.4967927789514116 34-35 0.4914154023429998 36-37 0.4479354714806991 38-39 0.4071442289226042 40-41 0.4011522949875168 42-43 0.41079316304974073 44-45 0.4450163241789898 46-47 0.4529287497599385 48-49 0.4502400614557327 50-51 0.4367966199347033 52-53 0.4294219320145958 54-55 0.43176493182254655 56-57 0.41463414634146345 58-59 0.41855194929902056 60-61 0.42934511234876127 62-63 0.429114653351258 64-65 0.4442481275206453 66-67 0.43230266948338775 68-69 0.4332629153063184 70-71 0.4432494718647974 72-73 0.445554061839831 74-75 0.4497023237948915 76-77 0.4404839638947571 78-79 0.43068945650086415 80-81 0.4388707509122335 82-83 0.4377952755905512 84-85 0.43599001344344146 86-87 0.4422892260418667 88-89 0.43015171884002307 90-91 0.4329556366429806 92-93 0.444363357019397 94-95 0.4415594392164394 96-97 0.40941040906472054 98-99 0.3819089686959862 100-101 0.3828692145189168 102-103 0.3643940848857307 104-105 0.3670059535241022 106-107 0.38498175532936435 108-109 0.3919339350873824 110-111 0.40906472056846555 112-113 0.405761474937584 114-115 0.4023045899750336 116-117 0.37657000192049167 118-119 0.450124831956981 120-121 0.45592471672748225 122-123 0.40902631073554835 124-125 0.4027270981371231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1301750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.7999217509814 #Duplication Level Percentage of deduplicated Percentage of total 1 89.25754495106212 48.91306479002909 2 7.538169244252041 8.261821694613328 3 1.5641169560556742 2.571404604037024 4 0.5415732205692264 1.1871268043848238 5 0.27708452486953966 0.7592105140629315 6 0.16161318615791298 0.5313833973228252 7 0.11670710448214798 0.44768781353837234 8 0.08121840656654304 0.35606098596687613 9 0.06421607318407517 0.3167132207078352 >10 0.3529388374896322 3.5220161888300607 >50 0.022711663940058923 0.8725865922160153 >100 0.015037980921098171 1.5978089970432245 >500 0.0022556969520968165 0.8094938918488961 >1k 0.0021053171552903624 3.660728663718993 >5k 0.0012030383744516355 4.207176184522213 >10k+ 0.0015037979680645444 21.985715657157577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 69863 5.366852314192434 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 49969 3.838601882081813 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 33627 2.583214903015172 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 22507 1.7289802189360475 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 17393 1.3361244478586518 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17260 1.3259074323026694 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16105 1.2371807182638754 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15139 1.1629729210677933 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 13660 1.0493566352986365 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11268 0.8656039946226234 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7567 0.5812944113693106 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7210 0.5538697906664106 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7058 0.5421932014595737 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6722 0.5163817937391972 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6455 0.4958709429613981 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5679 0.4362588822738621 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 5275 0.4052237372767428 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5087 0.3907816400998656 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4901 0.3764931822546572 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4895 0.3760322642596505 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4643 0.3566737084693682 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 4502 0.3458421355867102 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 4352 0.3343191857115422 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 4296 0.33001728442481276 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4099 0.3148838102554254 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3463 0.2660265027847129 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2392 0.18375264067601305 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2181 0.16754369118494333 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 1369 0.10516612252736701 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.681966583445362E-5 0.0 10-11 0.0 0.0 0.0 4.2250816208949486E-4 0.0 12-13 0.0 0.0 0.0 0.0011138851545995775 0.0 14-15 0.0 0.0 0.0 0.0026118686383714233 0.0 16-17 0.0 0.0 0.0 0.003879393124639908 0.0 18-19 0.0 0.0 0.0 0.005338966775494527 0.0 20-21 0.0 0.0 0.0 0.006990589590935279 0.0 22-23 0.0 0.0 0.0 0.011369310543499135 0.0 24-25 0.0 0.0 0.0 0.01567121183022854 0.0 26-27 0.0 0.0 0.0 0.01912809679277895 0.0 28-29 0.0 0.0 0.0 0.02615709621663146 0.0 30-31 0.0 0.0 0.0 0.03664298060303438 0.0 32-33 0.0 0.0 0.0 0.05511811023622047 0.0 34-35 0.0 0.0 0.0 0.08396389475705782 0.0 36-37 7.681966583445362E-5 0.0 0.0 0.1240637603226426 0.0 38-39 7.681966583445362E-5 0.0 0.0 0.189667754945266 0.0 40-41 7.681966583445362E-5 0.0 0.0 0.29402727098137127 0.0 42-43 7.681966583445362E-5 0.0 0.0 0.3701171499903975 0.0 44-45 7.681966583445362E-5 0.0 0.0 0.44989437295947765 0.0 46-47 7.681966583445362E-5 0.0 0.0 0.554100249663914 0.0 48-49 7.681966583445362E-5 0.0 0.0 0.6484732091415402 0.0 50-51 7.681966583445362E-5 0.0 0.0 0.7476089879009027 0.0 52-53 7.681966583445362E-5 0.0 0.0 0.8768580756673708 0.0 54-55 7.681966583445362E-5 0.0 0.0 1.0073746879201075 0.0 56-57 7.681966583445362E-5 0.0 0.0 1.1494526598809296 0.0 58-59 7.681966583445362E-5 0.0 0.0 1.3187632033800654 0.0 60-61 7.681966583445362E-5 0.0 0.0 1.469637027078932 0.0 62-63 7.681966583445362E-5 0.0 0.0 1.6212790474361438 0.0 64-65 7.681966583445362E-5 0.0 0.0 1.8043403111196468 0.0 66-67 7.681966583445362E-5 0.0 0.0 2.0097560975609756 0.0 68-69 7.681966583445362E-5 0.0 0.0 2.2670635682734783 0.0 70-71 7.681966583445362E-5 0.0 0.0 2.645861340503169 0.0 72-73 7.681966583445362E-5 0.0 0.0 2.933474169387363 0.0 74-75 7.681966583445362E-5 0.0 0.0 3.2143268676781256 0.0 76-77 7.681966583445362E-5 0.0 0.0 3.5512963318609563 0.0 78-79 7.681966583445362E-5 0.0 0.0 3.8628000768196658 0.0 80-81 7.681966583445362E-5 0.0 0.0 4.1857883618206255 0.0 82-83 7.681966583445362E-5 0.0 0.0 4.598156328019973 0.0 84-85 7.681966583445362E-5 0.0 0.0 4.990128672940273 0.0 86-87 7.681966583445362E-5 0.0 0.0 5.392394853082389 0.0 88-89 7.681966583445362E-5 3.840983291722681E-5 0.0 5.863645093143845 0.0 90-91 1.5363933166890725E-4 7.681966583445362E-5 0.0 6.303437680046091 0.0 92-93 1.5363933166890725E-4 7.681966583445362E-5 0.0 6.741194545803726 0.0 94-95 1.5363933166890725E-4 7.681966583445362E-5 0.0 7.223622047244095 0.0 96-97 1.5363933166890725E-4 7.681966583445362E-5 0.0 7.717149990397542 0.0 98-99 1.5363933166890725E-4 7.681966583445362E-5 0.0 8.29875168043019 0.0 100-101 1.5363933166890725E-4 7.681966583445362E-5 0.0 9.135241021701557 0.0 102-103 1.5363933166890725E-4 7.681966583445362E-5 0.0 9.821970424428653 0.0 104-105 1.9204916458613407E-4 7.681966583445362E-5 0.0 10.453965815248704 0.0 106-107 2.3045899750336087E-4 7.681966583445362E-5 0.0 11.122757826003458 0.0 108-109 2.3045899750336087E-4 7.681966583445362E-5 0.0 11.710927597464952 0.0 110-111 2.3045899750336087E-4 7.681966583445362E-5 0.0 12.332629153063184 0.0 112-113 2.3045899750336087E-4 7.681966583445362E-5 0.0 13.092759746495103 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1225 0.0 46.17511 5 ATAACGC 225 0.0 42.334145 3 TATAACG 205 0.0 40.651558 2 AAGTACT 1450 0.0 39.003983 4 TACGGGT 80 0.0053482023 29.766195 4 AAAGTAC 1900 0.0 27.886227 3 AAAAAGT 2015 0.0 26.590153 1 AAAAGTA 2030 0.0 26.097385 2 GGTGCGC 115 0.0010014696 25.88465 8 GTACACG 265 1.369699E-9 24.71156 1 CATGGGG 5785 0.0 23.874702 4 GTATAAC 465 0.0 23.044796 1 GTGCGCT 130 0.0020453567 22.897957 9 ATGGGGG 1870 0.0 22.6067 5 ATGGGAG 1965 0.0 22.422789 5 TGGGGGG 1030 0.0 21.386248 6 ATGGGGA 3425 0.0 21.035133 5 GTACTAG 175 4.7663393E-4 20.411106 1 GTATAGG 295 1.1514021E-7 20.180471 1 TATTGGG 295 1.1528027E-7 20.178133 2 >>END_MODULE