##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1301750 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.036704436335704 33.0 31.0 34.0 30.0 34.0 2 31.837162281544074 33.0 31.0 34.0 30.0 34.0 3 32.08984520837334 34.0 31.0 34.0 30.0 34.0 4 35.54388477050125 37.0 35.0 37.0 33.0 37.0 5 35.68198655655848 37.0 35.0 37.0 33.0 37.0 6 35.82427501440369 37.0 35.0 37.0 35.0 37.0 7 35.792099097368926 37.0 35.0 37.0 33.0 37.0 8 35.84556635298637 37.0 35.0 37.0 35.0 37.0 9 37.49307163433839 39.0 37.0 39.0 34.0 39.0 10-11 37.44463107355483 39.0 37.0 39.0 34.0 39.0 12-13 37.43412982523526 39.0 37.0 39.0 34.0 39.0 14-15 38.71781601690033 40.0 38.0 41.0 34.0 41.0 16-17 38.68209141540234 40.0 38.0 41.0 34.0 41.0 18-19 38.74739773381985 40.0 38.0 41.0 34.0 41.0 20-21 38.791872863453044 40.0 38.0 41.0 34.0 41.0 22-23 38.76826694833878 40.0 38.0 41.0 34.0 41.0 24-25 38.75231611292491 40.0 38.0 41.0 34.0 41.0 26-27 38.55036105242942 40.0 38.0 41.0 34.0 41.0 28-29 38.38359746495102 40.0 38.0 41.0 34.0 41.0 30-31 38.24086345304398 40.0 38.0 41.0 34.0 41.0 32-33 37.91716957941233 40.0 37.0 41.0 33.5 41.0 34-35 37.67657192241214 40.0 37.0 41.0 33.0 41.0 36-37 37.55636335701939 40.0 37.0 41.0 33.0 41.0 38-39 37.43406798540427 40.0 37.0 41.0 32.5 41.0 40-41 37.14698943729594 40.0 36.5 41.0 31.0 41.0 42-43 36.962235836374106 40.0 36.0 41.0 31.0 41.0 44-45 36.69778452083733 40.0 36.0 41.0 30.5 41.0 46-47 36.35725408104474 39.5 36.0 41.0 30.0 41.0 48-49 36.013612060687535 39.0 35.0 41.0 29.0 41.0 50-51 35.86024044555406 39.0 35.0 41.0 28.0 41.0 52-53 35.753420395621276 39.0 35.0 41.0 28.0 41.0 54-55 35.565320145957365 39.0 35.0 41.0 27.0 41.0 56-57 35.25685000960246 39.0 35.0 41.0 26.0 41.0 58-59 35.05881390435951 39.0 35.0 41.0 26.0 41.0 60-61 34.89625081620895 38.5 35.0 40.0 26.0 41.0 62-63 34.51742077971961 38.0 34.0 40.0 26.0 41.0 64-65 34.2350716343384 38.0 34.0 40.0 25.0 41.0 66-67 33.777934703284046 37.0 34.0 39.5 23.5 41.0 68-69 33.43165431150374 36.5 33.5 39.0 22.5 41.0 70-71 32.903175340887266 36.0 33.0 39.0 22.0 40.5 72-73 32.557038217783756 35.0 33.0 38.5 21.5 39.5 74-75 32.209410409064716 35.0 32.0 37.0 20.5 39.0 76-77 31.699890531976187 35.0 32.0 37.0 20.0 39.0 78-79 31.286660649126176 35.0 31.5 36.0 18.5 38.0 80-81 31.021226810063375 35.0 31.5 36.0 17.5 37.0 82-83 30.82844824275014 35.0 32.0 35.5 16.0 37.0 84-85 30.575877088534664 35.0 31.5 35.0 11.5 36.0 86-87 30.269854426733243 35.0 31.0 35.0 7.0 36.0 88-89 30.04909890531976 34.0 31.0 35.0 7.0 36.0 90-91 29.881214134818514 34.0 31.0 35.0 4.0 35.5 92-93 29.643228346456695 34.0 30.5 35.0 2.0 35.0 94-95 29.556147493758402 34.0 31.0 35.0 2.0 35.0 96-97 29.45387977722297 34.0 30.5 35.0 2.0 35.0 98-99 29.32594584213559 34.0 30.0 35.0 2.0 35.0 100-101 29.030711734203955 34.0 30.0 35.0 2.0 35.0 102-103 27.61665911273286 32.5 26.0 34.5 2.0 35.0 104-105 28.528846168619168 34.0 29.0 35.0 2.0 35.0 106-107 28.578458613405033 34.0 29.0 35.0 2.0 35.0 108-109 28.518917610908392 34.0 29.0 35.0 2.0 35.0 110-111 28.48979105050893 34.0 29.0 35.0 2.0 35.0 112-113 28.426313424236604 34.0 29.0 35.0 2.0 35.0 114-115 28.316535048972536 34.0 29.0 35.0 2.0 35.0 116-117 28.04377645477242 34.0 29.0 35.0 2.0 35.0 118-119 27.838771653543304 34.0 29.0 35.0 2.0 35.0 120-121 27.633537545611677 34.0 29.0 35.0 2.0 35.0 122-123 27.25456155175725 34.0 27.0 35.0 2.0 35.0 124-125 26.1590677933551 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39311236875607847 1101 2 0.7291581975626436 1101 3 0.5382202001278102 1101 4 0.5656709097761592 1101 5 0.3543928783482855 1101 6 0.2545066305806216 1101 7 0.2667699225532729 1101 8 0.3167666929315516 1101 9 0.3700807853459551 1101 10-11 0.3873665555603196 1101 12-13 0.39167186472845117 1101 14-15 0.39240723073938 1101 16-17 0.5320441587740845 1101 18-19 0.43520129313913003 1101 20-21 0.31027364833321514 1101 22-23 0.4475745176632273 1101 24-25 0.3596834695571758 1101 26-27 0.3946831736936005 1101 28-29 0.4968280409708683 1101 30-31 0.5006408807313463 1101 32-33 0.4716839345929529 1101 34-35 0.5505838356389816 1101 36-37 0.5203649726419002 1101 38-39 0.4431342841083179 1101 40-41 0.4078665060739226 1101 42-43 0.577853669055088 1101 44-45 0.5373577614964731 1101 46-47 0.44423883407555564 1101 48-49 0.4645294233647448 1101 50-51 0.4309616891131043 1101 52-53 0.4493663660178484 1101 54-55 0.5803843590966906 1101 56-57 0.44346145401309656 1101 58-59 0.6148900516699243 1101 60-61 0.4692906157542325 1101 62-63 0.5847109345004213 1101 64-65 0.5749688397139039 1101 66-67 0.5247743954802999 1101 68-69 0.6131066955938564 1101 70-71 0.5768210967474658 1101 72-73 0.6402843878962443 1101 74-75 0.5146045120191758 1101 76-77 0.46391524802514894 1101 78-79 0.5651258378816522 1101 80-81 0.5049974016912238 1101 82-83 0.561162242223574 1101 84-85 0.5961572086317979 1101 86-87 0.545535428012677 1101 88-89 0.49491702832222373 1101 90-91 0.49598724830057606 1101 92-93 0.4725237814929919 1101 94-95 0.528136268927252 1101 96-97 0.5966756534801192 1101 98-99 0.42732163749748864 1101 100-101 0.4536783555944801 1101 102-103 0.41365652887675 1101 104-105 0.5051654690964575 1101 106-107 0.3237336396365471 1101 108-109 0.3666821668760569 1101 110-111 0.3572031855304445 1101 112-113 0.46012792860339147 1101 114-115 0.18714315431109085 1101 116-117 0.31262186205976406 1101 118-119 0.39929653320644576 1101 120-121 0.20830597273679174 1101 122-123 0.27361841133073384 1101 124-125 0.3106077014558757 1102 1 0.3858169085042533 1102 2 0.6522341241454477 1102 3 0.5135057509522483 1102 4 0.5422295271293436 1102 5 0.3073457096435348 1102 6 0.24465066963797 1102 7 0.20946685452182123 1102 8 0.27326644538429434 1102 9 0.2977409291070785 1102 10-11 0.28431929829442026 1102 12-13 0.21811921536041723 1102 14-15 0.4346133313778253 1102 16-17 0.4004451165534988 1102 18-19 0.3669473798433245 1102 20-21 0.2381009041576405 1102 22-23 0.2629404188883413 1102 24-25 0.2945328472973259 1102 26-27 0.39296329732119517 1102 28-29 0.5095431609500309 1102 30-31 0.49500947506333404 1102 32-33 0.38623278855043 1102 34-35 0.42241559583423793 1102 36-37 0.49260988473318434 1102 38-39 0.5591153555757558 1102 40-41 0.6269148552799564 1102 42-43 0.5565349275844014 1102 44-45 0.5470778187597389 1102 46-47 0.732268428573839 1102 48-49 0.7257113284375478 1102 50-51 0.7197319233337964 1102 52-53 0.6603333925348522 1102 54-55 0.5786958088756862 1102 56-57 0.6842735880211919 1102 58-59 0.7287851076495215 1102 60-61 0.7305069089330942 1102 62-63 0.693155828973687 1102 64-65 0.6434135363377891 1102 66-67 0.6370890117914669 1102 68-69 0.5624293441186765 1102 70-71 0.5663201232814217 1102 72-73 0.4967036816022272 1102 74-75 0.5214574020568392 1102 76-77 0.5004839368537795 1102 78-79 0.39255207186115015 1102 80-81 0.3726958764138466 1102 82-83 0.46702244656429315 1102 84-85 0.5968469728000549 1102 86-87 0.5196893510596325 1102 88-89 0.5300617904719402 1102 90-91 0.6062110206569642 1102 92-93 0.6873341499309298 1102 94-95 0.6802723120433427 1102 96-97 0.6899851883597847 1102 98-99 0.6403727952225431 1102 100-101 0.6259303808419467 1102 102-103 0.49030997261003506 1102 104-105 0.6348963656791753 1102 106-107 0.7131035694518388 1102 108-109 0.7129563804601098 1102 110-111 0.7249227406331471 1102 112-113 0.7996429950690072 1102 114-115 0.8647083774781237 1102 116-117 0.6711091528281585 1102 118-119 0.6652292964509314 1102 120-121 0.5983031321850163 1102 122-123 0.5965098904762307 1102 124-125 0.52170886513996 1103 1 0.25390691677478827 1103 2 0.5952364631252216 1103 3 0.2974139957484141 1103 4 0.5306550751911772 1103 5 0.18159141640099108 1103 6 0.198212717806328 1103 7 0.17401447781181645 1103 8 0.2325533452184132 1103 9 0.3680554472865296 1103 10-11 0.24218404137228333 1103 12-13 0.27254560609794964 1103 14-15 0.42855000572486546 1103 16-17 0.3992022717690631 1103 18-19 0.2857805252990673 1103 20-21 0.20540986389236338 1103 22-23 0.26567707729654444 1103 24-25 0.25494961631746804 1103 26-27 0.26530505960015915 1103 28-29 0.22809355044416435 1103 30-31 0.27665583064938915 1103 32-33 0.2021169765626567 1103 34-35 0.40643441634634314 1103 36-37 0.442226163905616 1103 38-39 0.35731671159989276 1103 40-41 0.4455993212257354 1103 42-43 0.37763070938346033 1103 44-45 0.3067510996684675 1103 46-47 0.4477436856725632 1103 48-49 0.42169116308564725 1103 50-51 0.33940059012890345 1103 52-53 0.3506436180943737 1103 54-55 0.41993328335712476 1103 56-57 0.37517755808333675 1103 58-59 0.25974166683391076 1103 60-61 0.29589544197777684 1103 62-63 0.2733527687613204 1103 64-65 0.34654445467997164 1103 66-67 0.42272109668028435 1103 68-69 0.49713918361408105 1103 70-71 0.4309013222853366 1103 72-73 0.4435370027474832 1103 74-75 0.5128215608991766 1103 76-77 0.532445843021538 1103 78-79 0.43984481201297143 1103 80-81 0.3254217013904146 1103 82-83 0.39656970873990716 1103 84-85 0.27897903289763804 1103 86-87 0.31904332987229367 1103 88-89 0.34244645178173627 1103 90-91 0.4120550966716543 1103 92-93 0.32971804373115177 1103 94-95 0.3049323947306135 1103 96-97 0.2713415430560744 1103 98-99 0.3195716782357465 1103 100-101 0.38777849862093206 1103 102-103 0.14474410636454138 1103 104-105 0.33689752803733697 1103 106-107 0.4581496800234781 1103 108-109 0.4084091465850008 1103 110-111 0.44961343314186664 1103 112-113 0.5865945624978934 1103 114-115 0.5258928598381978 1103 116-117 0.49855556696229186 1103 118-119 0.4830799164469788 1103 120-121 0.5386024337513007 1103 122-123 0.5324119734609987 1103 124-125 0.5625125269077742 1104 1 0.30118728703507713 1104 2 0.6389021064192413 1104 3 0.20437839124163304 1104 4 0.5347643348367583 1104 5 0.3254385806495321 1104 6 0.24426125631572404 1104 7 0.12322761008903882 1104 8 0.19079775651452735 1104 9 0.2835256664039676 1104 10-11 0.28325990725446815 1104 12-13 0.3088981425913744 1104 14-15 0.4217629312352784 1104 16-17 0.4135777575326145 1104 18-19 0.2706722404113222 1104 20-21 0.26267884813193376 1104 22-23 0.1946698690731381 1104 24-25 0.293358859532475 1104 26-27 0.3336293761158444 1104 28-29 0.41704899496511416 1104 30-31 0.4278146017160651 1104 32-33 0.3670114331813181 1104 34-35 0.31624358626989135 1104 36-37 0.39401095842187317 1104 38-39 0.3407484822438107 1104 40-41 0.41438930152751396 1104 42-43 0.3991816497109326 1104 44-45 0.48199370452416446 1104 46-47 0.5839649390856039 1104 48-49 0.44419136154487404 1104 50-51 0.49920064477441883 1104 52-53 0.4947073567337341 1104 54-55 0.5832438738794465 1104 56-57 0.7148503172941503 1104 58-59 0.6305197931204276 1104 60-61 0.6063723117866857 1104 62-63 0.46706846531625246 1104 64-65 0.4647071884695464 1104 66-67 0.49355188388954474 1104 68-69 0.6315464966978084 1104 70-71 0.5210957292430436 1104 72-73 0.446019358338404 1104 74-75 0.5891425146849514 1104 76-77 0.6112965749206758 1104 78-79 0.456101045017018 1104 80-81 0.35508570070365764 1104 82-83 0.3314837389758587 1104 84-85 0.28485469496682114 1104 86-87 0.31232352382695083 1104 88-89 0.40029016007068563 1104 90-91 0.43098568302172424 1104 92-93 0.40081144325447937 1104 94-95 0.38089058352717586 1104 96-97 0.45710871716296 1104 98-99 0.4701245717711444 1104 100-101 0.4608817592597312 1104 102-103 0.39985950809215254 1104 104-105 0.43949505920466336 1104 106-107 0.48888328221520894 1104 108-109 0.45468949007636894 1104 110-111 0.44810387386902306 1104 112-113 0.2561985025133602 1104 114-115 0.3691313081271055 1104 116-117 0.5205254639296477 1104 118-119 0.5615933830587565 1104 120-121 0.4381794156204357 1104 122-123 0.48342531545884526 1104 124-125 0.6424201695261083 1105 1 0.2067747378736442 1105 2 0.5304147945397126 1105 3 0.13497183677735336 1105 4 0.44552172059846384 1105 5 0.15725121000232178 1105 6 0.103899353500573 1105 7 0.22601037798812484 1105 8 0.14419856786238938 1105 9 0.2262861359951316 1105 10-11 0.13123180784863564 1105 12-13 0.20617593587456184 1105 14-15 0.29620178827256183 1105 16-17 0.27112667819231007 1105 18-19 0.1866152848919711 1105 20-21 0.1542260548978831 1105 22-23 0.0882813154068316 1105 24-25 0.2096655167910697 1105 26-27 0.1695475131212092 1105 28-29 0.2646678472066384 1105 30-31 0.21819699460261432 1105 32-33 0.1433824499016083 1105 34-35 0.33629449697249925 1105 36-37 0.3840806443193969 1105 38-39 0.26897807619644 1105 40-41 0.33599708913047266 1105 42-43 0.32291448374822806 1105 44-45 0.42045408448969823 1105 46-47 0.29912321874510184 1105 48-49 0.2713460620753523 1105 50-51 0.2889476980368144 1105 52-53 0.37342664865189334 1105 54-55 0.5247129038981413 1105 56-57 0.43258275430561355 1105 58-59 0.28471814487352987 1105 60-61 0.3435528730475781 1105 62-63 0.3560261594609031 1105 64-65 0.347324261956679 1105 66-67 0.3731943346003632 1105 68-69 0.36171203387181095 1105 70-71 0.2758946808589542 1105 72-73 0.2639272385152154 1105 74-75 0.28517009422279216 1105 76-77 0.12764109213135555 1105 78-79 0.15502389129922634 1105 80-81 0.12255962868474057 1105 82-83 0.21361263774016592 1105 84-85 0.29477677864493046 1105 86-87 0.3341541716669951 1105 88-89 0.3141441508683336 1105 90-91 0.32995968077715077 1105 92-93 0.3272002475187712 1105 94-95 0.31398779786020015 1105 96-97 0.2985766170338273 1105 98-99 0.22825119451600884 1105 100-101 0.2918953003961313 1105 102-103 0.1544427488843958 1105 104-105 0.09777238470492478 1105 106-107 0.021028740101968424 1105 108-109 -0.02049774593584175 1105 110-111 0.053428368791216485 1105 112-113 0.09904186599052522 1105 114-115 0.16825686853301747 1105 116-117 0.20100712310458135 1105 118-119 0.1338955727938682 1105 120-121 0.20184007743500132 1105 122-123 0.16612157299889674 1105 124-125 0.036755893203981316 1106 1 0.17690732688088673 1106 2 0.3999091359288691 1106 3 0.3202666741996367 1106 4 0.45564304237111486 1106 5 0.21076346714733774 1106 6 0.12457363921215148 1106 7 0.047249636388031035 1106 8 -0.08393663610985413 1106 9 0.11389002210130172 1106 10-11 0.14962990369546247 1106 12-13 0.11148094128904518 1106 14-15 0.2640050020877638 1106 16-17 0.23375527948453367 1106 18-19 0.17192156485906196 1106 20-21 0.07315242674173561 1106 22-23 0.08555393865643168 1106 24-25 0.07582114629805403 1106 26-27 0.17229419768531073 1106 28-29 0.2674314705275478 1106 30-31 0.1777898863895544 1106 32-33 0.22864303076119086 1106 34-35 0.09776304217493248 1106 36-37 0.05675290969925584 1106 38-39 0.02069759691727313 1106 40-41 0.07730590777892843 1106 42-43 0.08864898970155366 1106 44-45 0.12893961985746216 1106 46-47 0.1330818251615966 1106 48-49 0.12751647212829909 1106 50-51 0.11229115277088653 1106 52-53 0.012084232038553466 1106 54-55 0.09874332590535317 1106 56-57 0.21250506819190207 1106 58-59 0.1668753322723333 1106 60-61 0.20930564442956268 1106 62-63 0.1031572907874434 1106 64-65 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2216 26-27 -1.2362687741851204 2216 28-29 -1.5725997301375756 2216 30-31 -1.3631028851732836 2216 32-33 -1.4748825707970852 2216 34-35 -1.3226598341233 2216 36-37 -1.263113353753596 2216 38-39 -1.4892313028190927 2216 40-41 -1.5985243619616014 2216 42-43 -1.6743493766653685 2216 44-45 -1.6072862432915898 2216 46-47 -1.8031640231712345 2216 48-49 -2.158982318994539 2216 50-51 -2.009011269767413 2216 52-53 -1.9537074176081504 2216 54-55 -2.031760065772822 2216 56-57 -2.2776659579906635 2216 58-59 -2.3078149610711023 2216 60-61 -2.4446671751543363 2216 62-63 -2.207408077606587 2216 64-65 -2.371915108670649 2216 66-67 -2.0301150927825447 2216 68-69 -2.1013596104090624 2216 70-71 -2.0671105018660043 2216 72-73 -1.8932718917584737 2216 74-75 -1.7719750548725166 2216 76-77 -1.6733933550697664 2216 78-79 -1.6268076454684 2216 80-81 -1.6638359325082597 2216 82-83 -1.886383875790731 2216 84-85 -1.8784261311976387 2216 86-87 -2.0094055960159807 2216 88-89 -2.032280430747985 2216 90-91 -2.1147514194609016 2216 92-93 -2.1311804707706834 2216 94-95 -2.2619702498707426 2216 96-97 -2.367565454218372 2216 98-99 -2.3782876191921964 2216 100-101 -2.392248326766776 2216 102-103 -2.401378576074322 2216 104-105 -2.2732736121637522 2216 106-107 -2.406368790946072 2216 108-109 -2.531581467582054 2216 110-111 -2.528066073688393 2216 112-113 -2.7528659269474858 2216 114-115 -2.7334029862230445 2216 116-117 -2.9125391980352724 2216 118-119 -3.0332327555653364 2216 120-121 -3.0063764370236505 2216 122-123 -3.0887607394637833 2216 124-125 -3.3680182943970145 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55.0 3 14.0 4 50.0 5 191.0 6 522.0 7 979.0 8 1616.0 9 3429.0 10 7643.0 11 10112.0 12 8367.0 13 9845.0 14 13448.0 15 13349.0 16 8437.0 17 7804.0 18 7986.0 19 7913.0 20 8163.0 21 7992.0 22 8305.0 23 8986.0 24 9839.0 25 11128.0 26 13072.0 27 16074.0 28 20009.0 29 24345.0 30 30590.0 31 38612.0 32 49538.0 33 65094.0 34 90018.0 35 133162.0 36 206702.0 37 313988.0 38 143329.0 39 1044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.48571762674885 17.86785285116636 11.045163533750713 23.601265988334074 2 12.428749650469983 23.287149279276786 36.32369705964595 27.960404010607277 3 22.633294052756835 29.075871944296356 28.915164709551632 19.375669293395177 4 13.790820049932783 18.89487228730555 33.64670635682735 33.66760130593432 5 11.223353178413674 39.275974649510275 34.910159400806606 14.590512771269445 6 31.573921567422392 29.21754869002477 20.203517850448335 19.005011892104502 7 22.789706164778185 32.57061647781832 24.287459189552525 20.352218167850967 8 28.101786057230647 32.483118878432876 23.379143460725945 16.035951603610524 9 29.21728442481275 14.371423084309583 21.85734588054542 34.55394661033224 10-11 23.70167993150756 27.490965043581735 26.66188848161647 22.145466543294237 12-13 27.216879757082683 25.34517958557323 26.442993752292715 20.994946905051368 14-15 27.867073612920674 22.797888652621744 26.18534747658497 23.149690257872614 16-17 22.429141213633404 26.61410384837572 32.421428483634266 18.535326454356618 18-19 24.622661801421163 24.875974649510272 32.19804109852122 18.30332245054734 20-21 20.702325143701064 25.577925058431074 33.44519578489684 20.274554012971024 22-23 19.850393700787404 27.332936431726523 27.549299020549263 25.267370846936814 24-25 20.969579412329555 28.950528135202614 26.36877280583829 23.711119646629538 26-27 23.249126176301132 23.913193777607066 27.75701939696562 25.080660649126173 28-29 22.34777571985954 23.671420022477484 31.153096569456277 22.827707688206694 30-31 25.069060879585177 25.45392740541579 29.707854810831574 19.769156904167467 32-33 19.178567313232186 25.691761090839254 31.8639907816401 23.26568081428846 34-35 17.41133758837626 27.390638521466688 32.865539798555716 22.33248409160134 36-37 23.707585942001153 25.311158056462453 29.002381409640872 21.978874591895526 38-39 25.425273670059532 23.983675821010177 28.82008834261571 21.770962166314575 40-41 21.839416877056607 26.576272931159185 29.073373947906262 22.51093624387795 42-43 24.831188784328788 25.555022085653928 28.171884002304594 21.441905127712694 44-45 26.67770309199155 22.884002304589977 28.28480891108124 22.153485692337238 46-47 22.59638947570578 25.113616285769158 33.622584981755324 18.667409256769734 48-49 24.90543499135779 24.959439216439407 31.092183599001345 19.04294219320146 50-51 20.628308046859996 26.169310543499137 31.8617630113309 21.340618398309967 52-53 19.92590743230267 28.041252160553103 26.16631457653159 25.866525830612634 54-55 21.14488188976378 29.08626848473209 25.052583061263682 24.716266564240446 56-57 23.391665066256962 24.461110044171306 26.286652583061265 25.860572306510466 58-59 22.724486268484732 24.03610524294219 29.437948914922217 23.801459573650856 60-61 25.045784520837334 25.914922220088343 28.164125216055307 20.875168043019013 62-63 19.519454580372575 26.17215287113501 30.17910505089303 24.129287497599385 64-65 17.71127328596121 27.452314192433263 31.434530439792585 23.401882081812943 66-67 23.523756481659305 25.8019973113117 27.718955252544653 22.955290954484347 68-69 25.100403303245635 24.492337238333015 27.67827923948531 22.728980218936048 70-71 21.83852506241598 26.73366621855195 27.87908584597657 23.548722873055503 72-73 24.52548492414058 25.9636642980603 27.062147109660074 22.448703668139043 74-75 26.25043211062032 23.36577683887075 27.288765123871713 23.09502592663722 76-77 22.302631073554828 25.403533704628384 32.60169003264836 19.69214518916843 78-79 24.37768388707509 25.18352218167851 30.336662185519494 20.102131745726908 80-81 20.247282504321106 26.348799692721336 31.096447090455154 22.3074707125024 82-83 19.753985020165164 27.909890531976185 25.8535048972537 26.48261955060495 84-85 20.775801805262148 28.77633954292299 24.97330516612253 25.474553485692336 86-87 22.834607259458423 24.439293259074326 26.076627616669867 26.649471864797388 88-89 22.194353754561167 24.070059535241022 28.92175917034761 24.8138275398502 90-91 24.344075283272517 25.776416362588822 27.697292106779337 22.182216247359325 92-93 19.309506433647012 26.112924908776648 29.326944497791434 25.250624159784905 94-95 17.726154466626337 27.289257626825687 30.54252489461201 24.442063011935968 96-97 23.08866205095211 25.636882184046016 27.170400530070964 24.104055234930907 98-99 24.382537270549697 24.539409294629017 27.091921474873587 23.9861319599477 100-101 21.38893796811984 26.451545995774918 27.48135202611869 24.678164009986556 102-103 23.756230801525852 25.606275185843725 26.87884329589379 23.758650716736632 104-105 25.26192000135234 23.358577030028883 27.1081726724177 24.271330296201075 106-107 21.680071670928402 25.38659120525177 31.786302616050357 21.147034507769472 108-109 23.681493988586094 25.29657525168414 29.382108480137077 21.639822279592696 110-111 19.667888953129893 26.410338661068096 30.476153889003072 23.445618496798943 112-113 19.18027767650967 27.709988970846695 25.682983487770933 27.4267498648727 114-115 20.01538598691653 28.707738490482516 24.828002803600413 26.448872719000544 116-117 21.912131675680165 24.80831111661032 25.69576320879928 27.583793998910235 118-119 21.1954970326381 24.556394091478577 28.254848496840157 25.993260379043164 120-121 23.177887756956174 25.980295176670754 27.11927499280671 23.722542073566363 122-123 18.773233915159096 26.48533809835854 28.321158294454897 26.42026969202747 124-125 17.345280063423633 27.76581500058 29.355054800445256 25.533850135551113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 419.0 1 397.0 2 1147.5 3 1514.0 4 783.5 5 501.0 6 749.5 7 1540.5 8 2289.0 9 2390.5 10 1969.5 11 1493.5 12 1393.0 13 1513.0 14 1748.0 15 2018.0 16 2211.0 17 2453.5 18 2776.0 19 2936.0 20 3167.0 21 3603.0 22 4052.0 23 4527.5 24 5148.5 25 5789.0 26 6701.0 27 7919.5 28 8691.5 29 9554.0 30 11003.5 31 12431.5 32 13947.5 33 15698.0 34 17427.5 35 18774.0 36 20460.5 37 22125.5 38 22561.5 39 22700.5 40 22919.0 41 23634.5 42 24509.5 43 25250.0 44 26690.5 45 28296.0 46 31083.0 47 39237.5 48 50396.0 49 86766.0 50 209166.0 51 210959.0 52 78869.0 53 30326.5 54 22844.5 55 19696.0 56 16824.5 57 14466.0 58 13015.0 59 11763.0 60 10068.5 61 8138.5 62 6630.5 63 5267.5 64 4088.5 65 3168.0 66 2264.0 67 1808.5 68 1423.5 69 918.0 70 664.5 71 559.5 72 483.5 73 420.0 74 306.5 75 240.5 76 149.5 77 56.0 78 31.0 79 21.5 80 8.5 81 1.0 82 1.0 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14565008642212407 2 1.5363933166890725E-4 3 3.072786633378145E-4 4 0.0 5 0.0 6 0.0035337046283848666 7 0.0 8 0.0 9 0.0 10-11 3.840983291722681E-5 12-13 0.005569425772997888 14-15 0.0016900326483579797 16-17 4.6091799500672174E-4 18-19 0.0 20-21 1.9204916458613405E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.3045899750336087E-4 30-31 0.0 32-33 0.0 34-35 3.840983291722681E-5 36-37 0.0 38-39 0.0 40-41 3.840983291722681E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.602458229306703E-4 96-97 0.002880737468792011 98-99 0.003994622623391588 100-101 0.0 102-103 0.003956212790474361 104-105 0.023276358747839447 106-107 0.01889763779527559 108-109 0.020549260610716345 110-111 0.0174764739773382 112-113 0.015325523333973497 114-115 0.04347993086230075 116-117 0.042481275206452854 118-119 0.02623391588246591 120-121 0.014634146341463414 122-123 0.0033032456308815057 124-125 0.0020741309775302477 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1301750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.49117042869338 #Duplication Level Percentage of deduplicated Percentage of total 1 88.30151883530361 46.350500742964 2 8.181720924751277 8.589362149222522 3 1.6754120006852729 2.6383301059874644 4 0.6060768631661934 1.2725473566937813 5 0.31297279833582836 0.8214154248495524 6 0.1880760684918455 0.5923399778858442 7 0.12266531751334551 0.4507192261097965 8 0.0876422897268168 0.3680357093449011 9 0.06920470355013762 0.3269372299665728 >10 0.3908450538187624 3.829214370147793 >50 0.031076827398471427 1.1100351041480874 >100 0.02388863143412823 2.641720183681064 >500 0.0035911012975602726 1.2381244662388806 >1k 0.002342022585365395 3.188865086177839 >5k 0.001561348390243597 5.723631319833502 >10k+ 0.0014052135512192371 20.85822154674837 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 62427 4.795621279047436 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 53958 4.145035529095448 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 33258 2.554868446322258 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 25903 1.989859804109852 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 18377 1.4117149990397542 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17105 1.3140003840983292 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16545 1.2709813712310352 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15656 1.2026886883042058 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11273 0.8659880929517958 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8675 0.6664106011138852 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8192 0.6293067025158441 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7637 0.5866717879777222 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7436 0.5712310351449972 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7277 0.559016708277319 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7049 0.5415018244670636 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6093 0.46806222392932595 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6074 0.46660265027847125 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5998 0.4607643556750528 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5406 0.41528711350105624 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4998 0.38394468984059915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4710 0.36182062608027654 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4376 0.336162857691569 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3806 0.2923756481659305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3249 0.24958709429613982 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3097 0.2379105050893029 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2867 0.22024198194737854 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2409 0.1850585749951988 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1855 0.14250048012291147 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1779 0.136662185519493 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.681966583445362E-5 0.0 0.0 2.6886883042058767E-4 0.0 12-13 7.681966583445362E-5 0.0 0.0 9.602458229306702E-4 0.0 14-15 7.681966583445362E-5 0.0 0.0 0.0024966391396197426 0.0 16-17 7.681966583445362E-5 0.0 0.0 0.0036873439600537735 0.0 18-19 7.681966583445362E-5 0.0 0.0 0.005185327443825619 0.0 20-21 7.681966583445362E-5 0.0 0.0 0.006952179758018052 0.0 22-23 7.681966583445362E-5 0.0 0.0 0.011599769541002496 0.0 24-25 7.681966583445362E-5 0.0 0.0 0.016093719992318033 0.0 26-27 7.681966583445362E-5 0.0 0.0 0.0196274246207029 0.0 28-29 7.681966583445362E-5 0.0 0.0 0.027040522373727675 0.0 30-31 7.681966583445362E-5 0.0 0.0 0.03810255425388899 0.0 32-33 7.681966583445362E-5 0.0 0.0 0.05711542154791627 0.0 34-35 7.681966583445362E-5 0.0 0.0 0.0859612060687536 0.0 36-37 7.681966583445362E-5 0.0 0.0 0.12602266180142116 0.0 38-39 7.681966583445362E-5 0.0 0.0 0.19162665642404456 0.0 40-41 7.681966583445362E-5 0.0 0.0 0.29775302477434223 0.0 42-43 7.681966583445362E-5 0.0 0.0 0.37368926445169964 0.0 44-45 7.681966583445362E-5 0.0 0.0 0.45461878240829656 0.0 46-47 7.681966583445362E-5 0.0 0.0 0.5597464951027462 0.0 48-49 7.681966583445362E-5 0.0 0.0 0.6548492414057998 0.0 50-51 7.681966583445362E-5 0.0 0.0 0.7550989053197619 0.0 52-53 7.681966583445362E-5 0.0 0.0 0.8850777799116574 0.0 54-55 7.681966583445362E-5 0.0 0.0 1.0160169003264836 0.0 56-57 7.681966583445362E-5 0.0 0.0 1.1594008066064911 0.0 58-59 7.681966583445362E-5 0.0 0.0 1.3304782024198194 0.0 60-61 7.681966583445362E-5 0.0 0.0 1.4823890916074516 0.0 62-63 7.681966583445362E-5 0.0 0.0 1.6360668331092758 0.0 64-65 7.681966583445362E-5 0.0 0.0 1.8200115229498752 0.0 66-67 7.681966583445362E-5 0.0 0.0 2.026541194545804 0.0 68-69 7.681966583445362E-5 0.0 0.0 2.284885730747071 0.0 70-71 7.681966583445362E-5 0.0 0.0 2.6652967159592853 0.0 72-73 7.681966583445362E-5 0.0 0.0 2.9536393316689074 0.0 74-75 7.681966583445362E-5 0.0 0.0 3.235413865949683 0.0 76-77 7.681966583445362E-5 0.0 0.0 3.573727674284617 0.0 78-79 7.681966583445362E-5 0.0 0.0 3.8878048780487804 0.0 80-81 1.9204916458613407E-4 0.0 0.0 4.213558671019781 0.0 82-83 2.3045899750336087E-4 0.0 0.0 4.629690800845017 0.0 84-85 2.3045899750336087E-4 0.0 0.0 5.024659112732859 0.0 86-87 2.3045899750336087E-4 0.0 0.0 5.430458997503361 0.0 88-89 2.3045899750336087E-4 0.0 0.0 5.90720184367198 0.0 90-91 2.3045899750336087E-4 0.0 0.0 6.350566545035528 0.0 92-93 2.3045899750336087E-4 0.0 0.0 6.792510082581141 0.0 94-95 2.3045899750336087E-4 0.0 0.0 7.281582485116189 0.0 96-97 2.3045899750336087E-4 0.0 0.0 7.782024198194738 0.0 98-99 2.3045899750336087E-4 0.0 0.0 8.372037641636258 0.0 100-101 2.3045899750336087E-4 0.0 0.0 9.21594008066065 0.0 102-103 2.3045899750336087E-4 0.0 0.0 9.910927597464951 0.0 104-105 2.3045899750336087E-4 0.0 0.0 10.550873823698868 0.0 106-107 2.3045899750336087E-4 0.0 0.0 11.230958325331287 0.0 108-109 2.3045899750336087E-4 0.0 3.840983291722681E-5 11.828922604186673 0.0 110-111 2.3045899750336087E-4 0.0 7.681966583445362E-5 12.463222584981756 0.0 112-113 2.3045899750336087E-4 0.0 7.681966583445362E-5 13.243019012867293 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 155 0.0 72.9365 3 TATAACG 225 0.0 50.24514 2 AGTACTC 1480 0.0 47.037815 5 AAAGTAC 1465 0.0 46.707134 3 TAACGCA 260 0.0 45.769863 4 AAGTACT 1715 0.0 40.592403 4 AAAAAGT 1985 0.0 33.908478 1 GTATAAC 395 0.0 33.175385 1 AAAAGTA 2135 0.0 32.049625 2 GTTATCA 755 0.0 30.768621 1 TATCACG 80 0.005362098 29.750414 2 CGCATGT 175 5.6473436E-7 27.229666 1 TTATCAA 980 0.0 24.28605 2 TTCAACG 240 3.5632365E-7 22.312809 2 GGGTAGG 360 2.1518645E-9 19.854963 1 TAGGGTA 245 1.0598957E-5 19.42884 5 ATAGGAC 215 8.562316E-5 19.372362 3 GTGTTAT 435 5.638867E-11 19.170311 1 CATATAG 225 1.21362675E-4 18.511368 3 ATAGTAC 390 5.8898877E-9 18.307947 3 >>END_MODULE