##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1449219 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02413368855915 33.0 31.0 34.0 30.0 34.0 2 31.808686609822256 33.0 31.0 34.0 30.0 34.0 3 32.07803789489373 34.0 31.0 34.0 30.0 34.0 4 35.51882082694196 37.0 35.0 37.0 33.0 37.0 5 35.658140005064794 37.0 35.0 37.0 33.0 37.0 6 35.8157538646678 37.0 35.0 37.0 33.0 37.0 7 35.78203984352952 37.0 35.0 37.0 33.0 37.0 8 35.845329794875724 37.0 35.0 37.0 35.0 37.0 9 37.494081294821555 39.0 37.0 39.0 34.0 39.0 10-11 37.43790517513226 39.0 37.0 39.0 34.0 39.0 12-13 37.43282036738408 39.0 37.0 39.0 34.0 39.0 14-15 38.695582586206775 40.0 38.0 41.0 34.0 41.0 16-17 38.651500566857045 40.0 38.0 41.0 34.0 41.0 18-19 38.70798512854165 40.0 38.0 41.0 34.0 41.0 20-21 38.74935051224142 40.0 38.0 41.0 34.0 41.0 22-23 38.73356269825334 40.0 38.0 41.0 34.0 41.0 24-25 38.71970971951099 40.0 38.0 41.0 34.0 41.0 26-27 38.507067944872375 40.0 38.0 41.0 34.0 41.0 28-29 38.31471502926749 40.0 38.0 41.0 34.0 41.0 30-31 38.1758640343523 40.0 38.0 41.0 34.0 41.0 32-33 37.82750019148244 40.0 37.0 41.0 33.0 41.0 34-35 37.592433234728496 40.0 37.0 41.0 33.0 41.0 36-37 37.481749135223865 40.0 37.0 41.0 33.0 41.0 38-39 37.35308983666374 40.0 37.0 41.0 32.5 41.0 40-41 37.034095260964705 40.0 36.0 41.0 31.0 41.0 42-43 36.84067521885926 40.0 36.0 41.0 30.5 41.0 44-45 36.574367987171016 40.0 36.0 41.0 30.0 41.0 46-47 36.22040112639981 39.0 35.0 41.0 30.0 41.0 48-49 35.87245060960421 39.0 35.0 41.0 28.5 41.0 50-51 35.72760811167946 39.0 35.0 41.0 28.0 41.0 52-53 35.62026753720452 39.0 35.0 41.0 27.5 41.0 54-55 35.408391002326084 39.0 35.0 41.0 27.0 41.0 56-57 35.06594551962126 39.0 35.0 41.0 26.0 41.0 58-59 34.82702303792594 38.0 34.0 40.0 25.5 41.0 60-61 34.63746059084238 38.0 34.0 40.0 26.0 41.0 62-63 34.22024069516063 38.0 34.0 40.0 24.0 41.0 64-65 33.94581046756909 37.5 34.0 40.0 22.5 41.0 66-67 33.480137232536975 37.0 33.5 39.0 22.5 41.0 68-69 33.109938180495845 36.0 33.0 39.0 22.0 41.0 70-71 32.533848231357716 35.5 32.0 39.0 21.0 40.0 72-73 32.18223815724194 35.0 32.0 37.5 20.5 39.0 74-75 31.80531410366549 35.0 32.0 37.0 19.5 39.0 76-77 31.261778240555774 35.0 31.0 36.5 18.0 38.5 78-79 30.846249945660386 35.0 31.0 36.0 15.0 37.0 80-81 30.549514255609402 35.0 31.0 35.5 11.5 37.0 82-83 30.43009062122426 34.5 31.0 35.0 9.0 36.5 84-85 30.192341185148692 34.0 31.0 35.0 7.0 36.0 86-87 29.862473511594864 34.0 30.5 35.0 6.5 36.0 88-89 29.647838594442938 34.0 30.0 35.0 2.0 35.5 90-91 29.456601452230476 34.0 30.0 35.0 2.0 35.0 92-93 29.19407177245123 34.0 29.5 35.0 2.0 35.0 94-95 29.133179664357147 34.0 30.0 35.0 2.0 35.0 96-97 29.046387744019363 34.0 29.5 35.0 2.0 35.0 98-99 28.911074516687954 34.0 29.0 35.0 2.0 35.0 100-101 28.575965399294375 34.0 29.0 35.0 2.0 35.0 102-103 27.069373572938254 32.0 25.5 34.0 2.0 35.0 104-105 27.963899521052376 34.0 27.0 35.0 2.0 35.0 106-107 28.02557860475194 34.0 28.0 35.0 2.0 35.0 108-109 27.958180578642704 34.0 28.0 35.0 2.0 35.0 110-111 27.89780323056764 34.0 27.0 35.0 2.0 35.0 112-113 27.894513182617672 34.0 29.0 35.0 2.0 35.0 114-115 27.763766207867825 34.0 29.0 35.0 2.0 35.0 116-117 27.441754834845526 34.0 27.0 35.0 2.0 35.0 118-119 27.21754579535598 34.0 27.0 35.0 2.0 35.0 120-121 26.97141770843468 34.0 26.0 35.0 2.0 35.0 122-123 26.523616858459626 34.0 25.0 35.0 2.0 35.0 124-125 25.36419202342779 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3757242923857831 1101 2 0.6367870933590929 1101 3 0.5016384154932965 1101 4 0.5988624056025174 1101 5 0.33103398146066354 1101 6 0.2539639041625108 1101 7 0.2523064041052123 1101 8 0.22915998888145594 1101 9 0.3308978041358799 1101 10-11 0.33366330615979223 1101 12-13 0.2852620324736037 1101 14-15 0.4115894412032688 1101 16-17 0.4612178263649014 1101 18-19 0.2867042937477109 1101 20-21 0.26345082541125464 1101 22-23 0.27170005441953293 1101 24-25 0.3522734906460414 1101 26-27 0.38374543532319194 1101 28-29 0.3723116737246528 1101 30-31 0.33502864676275834 1101 32-33 0.2876652039611898 1101 34-35 0.3511970208151709 1101 36-37 0.4953961281475472 1101 38-39 0.4391370673934887 1101 40-41 0.4336862069806102 1101 42-43 0.5471743674856029 1101 44-45 0.5535046464709765 1101 46-47 0.7862708276423831 1101 48-49 0.7412627415433022 1101 50-51 0.7868479508944475 1101 52-53 0.650092191063294 1101 54-55 0.5847889548320211 1101 56-57 0.6214477596227539 1101 58-59 0.7799000480023892 1101 60-61 0.7970762098260735 1101 62-63 0.6720530413264143 1101 64-65 0.5537148518063546 1101 66-67 0.5139995350110098 1101 68-69 0.5996061805479016 1101 70-71 0.6837999235166663 1101 72-73 0.5709790337716711 1101 74-75 0.6032933018346114 1101 76-77 0.6322051418096564 1101 78-79 0.5611971774163855 1101 80-81 0.6225582035574142 1101 82-83 0.6223015661740803 1101 84-85 0.5712303683710864 1101 86-87 0.55180788659775 1101 88-89 0.5812635757323612 1101 90-91 0.6933528290164901 1101 92-93 0.6178943334149736 1101 94-95 0.6322211622087153 1101 96-97 0.5660581974396628 1101 98-99 0.6171618643854302 1101 100-101 0.7324310135021861 1101 102-103 0.5340899418190723 1101 104-105 0.6190160455385616 1101 106-107 0.6000067510809508 1101 108-109 0.5856334165220183 1101 110-111 0.6091687082170729 1101 112-113 0.5557082633194277 1101 114-115 0.5291255959823253 1101 116-117 0.7444952167711918 1101 118-119 0.8241958357396406 1101 120-121 0.642337424090865 1101 122-123 0.8183245536407782 1101 124-125 0.7592678070728844 1102 1 0.35414414349457246 1102 2 0.6317487458818185 1102 3 0.446761567425078 1102 4 0.5338638755727061 1102 5 0.2882896161819488 1102 6 0.3103201933658255 1102 7 0.24906063100941367 1102 8 0.23550670848020872 1102 9 0.4065144516962249 1102 10-11 0.34675324268471286 1102 12-13 0.35427072009606064 1102 14-15 0.4573262316960154 1102 16-17 0.43460643320805303 1102 18-19 0.36207035578637203 1102 20-21 0.3497003888597092 1102 22-23 0.4582668946544928 1102 24-25 0.4282235945305146 1102 26-27 0.4408466738470622 1102 28-29 0.5673364659063864 1102 30-31 0.5583371451635486 1102 32-33 0.5936445296690991 1102 34-35 0.626412139606515 1102 36-37 0.6663308713368394 1102 38-39 0.7594553110060218 1102 40-41 0.7181076799893944 1102 42-43 0.6784748668808405 1102 44-45 0.6332139278035456 1102 46-47 0.7873788300022255 1102 48-49 0.8477854629415305 1102 50-51 0.8316945708509209 1102 52-53 0.9097104329000274 1102 54-55 0.8309477944829382 1102 56-57 0.8737897476933014 1102 58-59 0.7513965368454194 1102 60-61 0.7100470689003231 1102 62-63 0.772096943120566 1102 64-65 0.808432666858458 1102 66-67 0.6825035986399968 1102 68-69 0.7593246565933143 1102 70-71 0.79465649191566 1102 72-73 0.7250050749347849 1102 74-75 0.7274879258872673 1102 76-77 0.6347412843316498 1102 78-79 0.7551802638802272 1102 80-81 0.7605479552015133 1102 82-83 0.7257803640738985 1102 84-85 0.7355554744753086 1102 86-87 0.7096417424775332 1102 88-89 0.6606109878942483 1102 90-91 0.8422946477938353 1102 92-93 0.7593440397169005 1102 94-95 0.7416531420513763 1102 96-97 0.8591568353988315 1102 98-99 0.8245957699876953 1102 100-101 0.8264520824171484 1102 102-103 0.8709073675757146 1102 104-105 0.951242823462568 1102 106-107 0.937741181728942 1102 108-109 0.9256013139516419 1102 110-111 0.8556977041189135 1102 112-113 0.8910601583970994 1102 114-115 0.8684208587320974 1102 116-117 0.8154750456232556 1102 118-119 0.7746934551771858 1102 120-121 0.6800426526465664 1102 122-123 0.7615870587462119 1102 124-125 0.736441735129187 1103 1 0.2655475672881522 1103 2 0.5842508756408158 1103 3 0.39277125295978976 1103 4 0.5326967776277627 1103 5 0.20025551606879333 1103 6 0.24944813570403568 1103 7 0.27237662332730395 1103 8 0.23793018946018663 1103 9 0.3074629310033572 1103 10-11 0.32357397440298996 1103 12-13 0.333263756058777 1103 14-15 0.46613938916235753 1103 16-17 0.4257762975925914 1103 18-19 0.4037352330892858 1103 20-21 0.2851633480312543 1103 22-23 0.324543496667701 1103 24-25 0.2057634007998459 1103 26-27 0.2650722954720308 1103 28-29 0.3244731310183866 1103 30-31 0.3901624859499577 1103 32-33 0.3779709016283874 1103 34-35 0.40411433687863507 1103 36-37 0.30363935531795505 1103 38-39 0.3729978817175237 1103 40-41 0.35323577013737406 1103 42-43 0.29225373347953365 1103 44-45 0.2638075538068776 1103 46-47 0.28042358140716317 1103 48-49 0.32034188282912623 1103 50-51 0.4021445017897989 1103 52-53 0.34555839657078025 1103 54-55 0.4804593730812883 1103 56-57 0.47571427083202167 1103 58-59 0.24049886215274796 1103 60-61 0.27372261846802814 1103 62-63 0.28745248054390515 1103 64-65 0.3560826413412954 1103 66-67 0.34544119794968964 1103 68-69 0.33925119114334024 1103 70-71 0.4231079748571176 1103 72-73 0.3491956416753865 1103 74-75 0.41144886815457227 1103 76-77 0.2935744660268078 1103 78-79 0.3812698865358719 1103 80-81 0.3007002791605764 1103 82-83 0.23993255021034088 1103 84-85 0.24830117076419 1103 86-87 0.19668441855342422 1103 88-89 0.2736528030781038 1103 90-91 0.3878733879552456 1103 92-93 0.19697810467530275 1103 94-95 0.2278981447329258 1103 96-97 0.28117689558137826 1103 98-99 0.29525722869034254 1103 100-101 0.2663545279697246 1103 102-103 0.23549084863559244 1103 104-105 0.4146165614617594 1103 106-107 0.36875866223153153 1103 108-109 0.40698317549583507 1103 110-111 0.5599094835587799 1103 112-113 0.6512719658009694 1103 114-115 0.4820890677470935 1103 116-117 0.5734590202479133 1103 118-119 0.5880264955542209 1103 120-121 0.7019249900782931 1103 122-123 0.580543262112478 1103 124-125 0.5215160444930369 1104 1 0.21990569330593956 1104 2 0.572253510507263 1104 3 0.13182978987178018 1104 4 0.41750982762899724 1104 5 0.1521278417602403 1104 6 0.10014695478507463 1104 7 0.10991216730293019 1104 8 0.1318506057641713 1104 9 0.23613356635057414 1104 10-11 0.1713859684509913 1104 12-13 0.19956691026091278 1104 14-15 0.26792414874922343 1104 16-17 0.2988742457016116 1104 18-19 0.2218925916213479 1104 20-21 0.15971233600952672 1104 22-23 0.20637297199388627 1104 24-25 0.1489027659461044 1104 26-27 0.255987452895468 1104 28-29 0.28365635108809073 1104 30-31 0.2160867419349728 1104 32-33 0.25878732117906367 1104 34-35 0.2678120078639097 1104 36-37 0.3082092145999553 1104 38-39 0.3041906624947046 1104 40-41 0.27635666173293316 1104 42-43 0.2623986368005333 1104 44-45 0.2917112767535812 1104 46-47 0.3986185750380713 1104 48-49 0.595758423311743 1104 50-51 0.5191267975267309 1104 52-53 0.4546394715422295 1104 54-55 0.4815280215082396 1104 56-57 0.5641944291697243 1104 58-59 0.5764058383445558 1104 60-61 0.533809920430727 1104 62-63 0.5182098981700278 1104 64-65 0.3666376689543611 1104 66-67 0.3762351244883462 1104 68-69 0.5011450802311899 1104 70-71 0.4192972675281581 1104 72-73 0.38933640267838854 1104 74-75 0.40856460263587735 1104 76-77 0.3317106153992455 1104 78-79 0.1817034273127227 1104 80-81 0.14263194258484901 1104 82-83 0.17120201403713153 1104 84-85 0.08893331756810241 1104 86-87 0.07237566659104289 1104 88-89 0.139514697008142 1104 90-91 0.0578637375623785 1104 92-93 5.0241258774264E-4 1104 94-95 0.09063282404318329 1104 96-97 0.15401367084720974 1104 98-99 0.12004014604116975 1104 100-101 0.0602047931046954 1104 102-103 -0.021149299414908285 1104 104-105 0.12086427453570181 1104 106-107 0.10074300865135655 1104 108-109 0.12581335868129884 1104 110-111 0.09233167073954363 1104 112-113 0.11288918162679806 1104 114-115 0.12390530731272165 1104 116-117 0.0753883821568948 1104 118-119 0.14275618114962185 1104 120-121 0.0528167965405153 1104 122-123 -0.030922461178633398 1104 124-125 -0.10693103263372805 1105 1 0.2740242550072338 1105 2 0.5064350094366112 1105 3 0.1704439219451146 1105 4 0.3800310322567526 1105 5 0.18862721896424262 1105 6 0.12463904382216384 1105 7 0.1666510970383115 1105 8 0.14623413920596562 1105 9 0.3000310886509254 1105 10-11 0.21722884667198628 1105 12-13 0.1541113890958954 1105 14-15 0.2608470280083708 1105 16-17 0.30504434599098573 1105 18-19 0.18024741497774244 1105 20-21 0.2561796591732275 1105 22-23 0.261547837848326 1105 24-25 0.17807202068478034 1105 26-27 0.17954297696810784 1105 28-29 0.32422239195357605 1105 30-31 0.33833672637238976 1105 32-33 0.20455108879526307 1105 34-35 0.29972876571072504 1105 36-37 0.2961430910917926 1105 38-39 0.27590647472196395 1105 40-41 0.23199013102176025 1105 42-43 0.3010209648418041 1105 44-45 0.43744505102998943 1105 46-47 0.4460942071569747 1105 48-49 0.36162498788006303 1105 50-51 0.4067504176038099 1105 52-53 0.43269359654362916 1105 54-55 0.4283115041350243 1105 56-57 0.517883697779105 1105 58-59 0.5178226206816987 1105 60-61 0.5454464386196278 1105 62-63 0.43520201306344575 1105 64-65 0.49196475149614827 1105 66-67 0.5015741106978524 1105 68-69 0.4144445212856951 1105 70-71 0.5809413669874104 1105 72-73 0.5526155001696154 1105 74-75 0.619166926270843 1105 76-77 0.6364763170687446 1105 78-79 0.5371267480808584 1105 80-81 0.6027983675680311 1105 82-83 0.6561473869351353 1105 84-85 0.6045551251654153 1105 86-87 0.5773522778354057 1105 88-89 0.620813644743432 1105 90-91 0.6126291953042653 1105 92-93 0.6895265089286156 1105 94-95 0.7007793152614994 1105 96-97 0.6432764649970864 1105 98-99 0.7282445831430664 1105 100-101 0.738747124596081 1105 102-103 0.593112055562024 1105 104-105 0.6918815561243754 1105 106-107 0.7578987423846542 1105 108-109 0.6623050277726037 1105 110-111 0.7732752256937978 1105 112-113 0.9793097350849003 1105 114-115 1.0020142867566477 1105 116-117 0.8276709104412774 1105 118-119 0.8554446713371924 1105 120-121 0.9941560098954163 1105 122-123 0.8524421827036335 1105 124-125 0.8115698233570647 1106 1 0.1700234948696533 1106 2 0.46914334712181827 1106 3 0.3901381033617852 1106 4 0.45727483843867844 1106 5 0.1791759654674152 1106 6 0.07396825752527292 1106 7 0.03689235753572717 1106 8 0.010257503824107062 1106 9 0.20966067501000651 1106 10-11 0.08250675256687146 1106 12-13 0.15931265641088999 1106 14-15 0.26726531035253487 1106 16-17 0.24245962309274915 1106 18-19 0.16278846693644056 1106 20-21 0.05739018567386722 1106 22-23 0.11194658657904455 1106 24-25 0.06265089577358651 1106 26-27 0.13366649230127337 1106 28-29 0.18130922380844794 1106 30-31 0.13464989270590877 1106 32-33 0.13284203441418896 1106 34-35 -0.008645392051462863 1106 36-37 -4.8341016606912035E-4 1106 38-39 0.060069825183653336 1106 40-41 0.07067495989929995 1106 42-43 0.15157536431370033 1106 44-45 0.2014568989721468 1106 46-47 0.19407218017394 1106 48-49 0.12383308035065 1106 50-51 0.1588948208227876 1106 52-53 0.1773627897311485 1106 54-55 0.3117037733952799 1106 56-57 0.23556181389493247 1106 58-59 0.30673387332448243 1106 60-61 0.37159324906420466 1106 62-63 0.25717804913449527 1106 64-65 0.143200982552095 1106 66-67 0.22524330148222305 1106 68-69 0.23292063398052676 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2216 28-29 -1.4124714766763091 2216 30-31 -1.1337287833993202 2216 32-33 -1.2175943821968787 2216 34-35 -1.0546844660859236 2216 36-37 -1.0501333561630162 2216 38-39 -1.180448115960992 2216 40-41 -1.3334774419442041 2216 42-43 -1.4823533770200044 2216 44-45 -1.555731735762457 2216 46-47 -1.6723230584073292 2216 48-49 -1.6740037410286703 2216 50-51 -1.6999637280662725 2216 52-53 -1.8234430754682194 2216 54-55 -1.8751353762953613 2216 56-57 -2.034659155592145 2216 58-59 -2.1467427646578017 2216 60-61 -2.206737617177936 2216 62-63 -1.9806785666391704 2216 64-65 -2.0491811934204165 2216 66-67 -2.074173726886734 2216 68-69 -2.122885141838445 2216 70-71 -2.1580636946985123 2216 72-73 -2.088752475091095 2216 74-75 -1.988567563524633 2216 76-77 -1.9366606081355933 2216 78-79 -1.8622335621556942 2216 80-81 -1.8579405649983656 2216 82-83 -1.9619052381516973 2216 84-85 -1.9713138985719247 2216 86-87 -2.0447719855800095 2216 88-89 -2.130997114422893 2216 90-91 -2.2171139982538293 2216 92-93 -2.2963910109102095 2216 94-95 -2.35298080973336 2216 96-97 -2.5994312664630534 2216 98-99 -2.5436008439191014 2216 100-101 -2.7650096461391946 2216 102-103 -2.7048435773218387 2216 104-105 -2.9067369217342645 2216 106-107 -3.089335291880733 2216 108-109 -3.195787069375406 2216 110-111 -3.1058837856868777 2216 112-113 -3.2743642611799686 2216 114-115 -3.3862704747338555 2216 116-117 -3.447393523803335 2216 118-119 -3.644020811167639 2216 120-121 -3.747457800132306 2216 122-123 -3.708491729726383 2216 124-125 -3.993423262540116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51.0 3 16.0 4 60.0 5 193.0 6 615.0 7 1101.0 8 1917.0 9 4108.0 10 9080.0 11 11681.0 12 9648.0 13 10959.0 14 14452.0 15 14594.0 16 9847.0 17 9511.0 18 9927.0 19 9957.0 20 10155.0 21 9939.0 22 10167.0 23 10944.0 24 12190.0 25 13640.0 26 16222.0 27 19607.0 28 24264.0 29 29829.0 30 36685.0 31 45664.0 32 58186.0 33 77029.0 34 106650.0 35 157521.0 36 237846.0 37 328092.0 38 126051.0 39 821.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.90162434668207 17.08859972305677 10.337417462901492 23.67235846735967 2 11.955446193591838 23.175390246023017 35.413955426872775 29.45520813351237 3 23.613246371131346 29.30544323397819 28.4985909583967 18.582719436493765 4 14.106494601575056 18.844080846304113 33.19498295288704 33.854441599233795 5 11.12585468448868 39.029090841342814 34.38182910933406 15.463225364834438 6 31.658564355581625 27.354529301154106 20.66920834267773 20.31769800058654 7 22.315743859278687 32.196100106333134 23.901425526438725 21.586730507949454 8 28.53716381030058 31.993439224851457 23.59905576727879 15.870341197569173 9 29.791425588541138 14.498774857354203 22.09983446256225 33.609965091542406 10-11 23.918252436228514 27.481244188674232 26.056337299266673 22.544166075830578 12-13 27.662560095946496 25.422025876018967 25.431583262084228 21.483830765950312 14-15 28.764697764283742 22.058239028429476 25.86809964118134 23.308963566105437 16-17 22.98438737741614 25.910943326442233 32.93500191828387 18.16966737785776 18-19 25.66334004729444 24.163566721109785 31.838286690969415 18.334806540626364 20-21 20.739785691169786 25.174628782301063 33.54614940343647 20.539436123092678 22-23 19.967237525867382 27.71803295430159 26.31990058093359 25.994828938897435 24-25 21.098571023427102 29.4174310438933 25.170005361508508 24.31399257117109 26-27 23.700800224120716 23.36637871846836 26.382934532323958 26.54988652508696 28-29 22.7077261084614 23.346243762144496 30.365245760466347 23.580784368927752 30-31 26.303477942257175 25.050285705611092 29.015145399004567 19.631090953127167 32-33 19.097596705535878 25.317809109596272 31.67002364721964 23.91457053764821 34-35 16.90836023289802 27.22813268949185 32.760047142665904 23.103459934944226 36-37 24.214076685442297 25.087857666784664 27.938013509345378 22.760052138427664 38-39 26.378449357895526 23.57235172875873 28.115419408660802 21.933779504684935 40-41 22.35249550016095 26.51549783555758 28.220382226696668 22.91162443758481 42-43 25.602962699219372 25.455400460523908 27.038218516318103 21.903418323938617 44-45 27.696849130462688 22.08913904661752 27.615460465257495 22.5985513576623 46-47 23.01467204059566 24.670839948965614 33.81507556828885 18.499412442149875 48-49 25.827738940767407 24.16128963255381 30.989346675692218 19.021624750986565 50-51 20.586812621142837 25.62883870553726 32.26613783010021 21.51821084321969 52-53 20.00349843605418 28.18324904655542 25.240457101376673 26.57279541601373 54-55 21.244925715161063 29.353534558958998 24.193030866970417 25.20850885890952 56-57 23.82717863897727 23.74371989326665 25.14285280554561 27.286248662210472 58-59 23.019916244542753 23.617997003903483 28.923509835297494 24.438576916256274 60-61 26.210841839639144 25.427419872358836 27.550080422627637 20.811657865374382 62-63 19.404003121681402 25.766188547072595 30.07154198226769 24.75826634897831 64-65 17.20585363564789 27.221489643732244 31.43689808096637 24.135758639653496 66-67 24.01258884958036 25.404994000216668 26.78366761683362 23.798749533369353 68-69 25.910887174402212 23.994751655891896 27.139617959742456 22.954743209963436 70-71 22.182120162653128 26.532083832740256 27.235773199219715 24.0500228053869 72-73 25.193914791346234 25.72268235511679 26.087706550907765 22.99569630262921 74-75 27.215486410266493 22.353005308376442 26.676230438601756 23.755277842755305 76-77 22.77768232406558 24.811881434068972 32.79000620334125 19.620430038524198 78-79 25.27292286397018 24.456207101894194 30.163591562075847 20.10727847205978 80-81 20.363036918505763 25.72709852686171 31.394495931946793 22.515368622685735 82-83 19.86970223271983 28.0462097170959 24.9328776396114 27.15121041057287 84-85 21.029154323811653 29.077213312825734 24.00537806915311 25.8882542942095 86-87 23.231374968172513 23.758003448754124 24.97928194427481 28.031339638798553 88-89 22.535344899563146 23.54544068218813 28.438938490317888 25.48027592793084 90-91 25.385224731389805 25.287654936900495 27.19709719510992 22.13002313659978 92-93 19.256647891036483 25.62173142913528 29.2115615376282 25.910059142200044 94-95 17.259611635880866 26.981514718049247 30.629406289094657 25.129467356975226 96-97 23.51415161879421 25.190478342759924 26.47058113188799 24.824788906557878 98-99 25.187227732269 23.957731696259447 26.597585792152895 24.25745477931866 100-101 21.79139246725305 26.096849406473417 26.921500477153554 25.190257649119975 102-103 24.30651995623667 25.349125026696527 26.052429737671893 24.291925279394906 104-105 26.15329879240173 22.33773825685395 26.62103003018133 24.88793292056299 106-107 22.19660635147068 24.658421507945462 32.037621167842154 21.1073509727417 108-109 24.484976060043998 24.55054134495104 29.249122201613254 21.71536039339171 110-111 19.928722347386767 25.755884391898725 30.712193357886214 23.603199902828297 112-113 19.389204717005548 27.635786440765692 24.888984739607146 28.086024102621614 114-115 20.237448886222726 28.820214461277093 24.05283974633553 26.889496906164652 116-117 22.438787664582737 24.046000847009804 24.77061876930925 28.744592719098204 118-119 21.596824310054235 23.91630236278731 27.82169286387711 26.665180463281345 120-121 24.136084373830034 25.427536744471723 26.758291336775876 23.67808754492237 122-123 18.907958352870306 25.87467839471479 28.240057881188303 26.977305371226606 124-125 17.23346292225079 27.199112888645992 29.3418152660989 26.225608923004312 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 348.0 1 326.0 2 1104.0 3 1545.0 4 825.0 5 502.5 6 692.0 7 1286.5 8 1887.0 9 1987.5 10 1708.0 11 1364.0 12 1331.5 13 1529.5 14 1795.0 15 2104.0 16 2295.5 17 2575.0 18 2877.0 19 2944.5 20 3194.0 21 3614.5 22 4080.0 23 4600.0 24 5121.0 25 5719.5 26 6685.5 27 7865.0 28 8545.5 29 9227.0 30 10471.5 31 12001.0 32 13467.0 33 14773.0 34 16458.5 35 17711.0 36 18866.0 37 19893.5 38 19770.5 39 19456.0 40 19344.5 41 20213.0 42 21211.5 43 22207.5 44 23897.0 45 25780.0 46 29446.5 47 39594.0 48 54137.0 49 102532.5 50 263010.5 51 271198.5 52 103855.0 53 38249.0 54 25853.5 55 21131.0 56 18124.5 57 15741.0 58 14527.5 59 13208.0 60 11188.0 61 9199.0 62 7530.5 63 6027.0 64 4913.5 65 3848.0 66 2642.5 67 2077.0 68 1565.0 69 980.0 70 790.5 71 667.0 72 602.5 73 537.5 74 377.0 75 283.5 76 185.0 77 85.5 78 45.0 79 18.0 80 10.0 81 5.5 82 2.5 83 0.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1373843428770945 2 3.4501341757180935E-4 3 4.8301878460053313E-4 4 0.0 5 0.0 6 0.0030361180746319226 7 0.0 8 0.0 9 0.0 10-11 1.0350402527154281E-4 12-13 0.005382209314120226 14-15 0.0013110509867728757 16-17 4.8301878460053313E-4 18-19 0.0 20-21 1.3800536702872376E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.0700805054308562E-4 30-31 0.0 32-33 0.0 34-35 6.900268351436188E-5 36-37 0.0 38-39 0.0 40-41 3.450134175718094E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.280322021723425E-4 96-97 0.002656603315302932 98-99 0.00331212880868937 100-101 0.0 102-103 0.004174662352618893 104-105 0.02139083188945218 106-107 0.017078164169804563 108-109 0.01942425540929287 110-111 0.016388137334660945 112-113 0.014249054145715727 114-115 0.042264143652546646 116-117 0.04095309266577377 118-119 0.02325390434433995 120-121 0.013214013893000299 122-123 0.0030016167328747413 124-125 0.0014490563538015993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1449219.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.994989627297294 #Duplication Level Percentage of deduplicated Percentage of total 1 88.77371604623444 42.60693580815657 2 7.791055636723519 7.478632689404829 3 1.587944912017372 2.286401987429799 4 0.6045636755417318 1.1606410934666458 5 0.3119561182689895 0.748616533024604 6 0.1826471560077284 0.5259689014827765 7 0.12476005254702333 0.41915001995268153 8 0.09271464016359168 0.35598705543601467 9 0.06886352787131857 0.297459387529473 >10 0.39654306247072624 3.5137183114383386 >50 0.030673550850447158 0.9937118819058101 >100 0.0239701579848195 2.405438337610827 >500 0.004180840783555602 1.4080270802516106 >1k 0.0032053112673926286 3.4608397314321806 >5k 0.0012542522350666805 4.890069822678032 >10k+ 0.0019510590323259477 27.448401358799778 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 81884 5.650215736890008 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 68799 4.7473156231045825 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 43359 2.9918873544992164 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 39891 2.7525860480714095 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 26201 1.8079393107597954 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 24466 1.6882196548623778 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 22223 1.5334466357396639 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 21447 1.4799005533325191 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 19615 1.3534876371342082 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 16061 1.108252099924166 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13824 0.9538930969025385 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 11346 0.7829044471539499 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 10693 0.7378456948190716 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 10564 0.7289443486457189 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9884 0.6820225238559527 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9699 0.6692570274057957 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9089 0.627165390462035 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 8725 0.6020484136628074 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7939 0.547812304420519 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7574 0.5226263249377768 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7313 0.5046166245405284 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6934 0.4784646074885852 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 5953 0.4107729749609963 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4706 0.324726628618587 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4085 0.2818759621561682 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3955 0.2729056132993012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3793 0.2617271785699746 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 3702 0.25544793437016766 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 3307 0.2281918743819947 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3107 0.21439133767912236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.2014188331784223 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 2377 0.16401937871363817 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2339 0.16139727674009244 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1869 0.12896601548834233 No Hit ATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 1661 0.11461345731735507 No Hit AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA 1503 0.10371103332208591 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.900268351436188E-5 0.0 0.0 0.0 0.0 8 6.900268351436188E-5 0.0 0.0 0.0 0.0 9 6.900268351436188E-5 0.0 0.0 0.0 0.0 10-11 6.900268351436188E-5 0.0 0.0 1.0350402527154281E-4 0.0 12-13 6.900268351436188E-5 0.0 0.0 7.935308604151615E-4 0.0 14-15 6.900268351436188E-5 0.0 0.0 0.002242587214216761 0.0 16-17 6.900268351436188E-5 0.0 0.0 0.004968193213034055 0.0 18-19 6.900268351436188E-5 0.0 0.0 0.006865767009679006 0.0 20-21 6.900268351436188E-5 0.0 0.0 0.00924635959092449 0.0 22-23 6.900268351436188E-5 0.0 0.0 0.013938542069901099 0.0 24-25 6.900268351436188E-5 0.0 0.0 0.01904474064996388 0.0 26-27 6.900268351436188E-5 0.0 0.0 0.023736923128940483 0.0 28-29 6.900268351436188E-5 0.0 0.0 0.03205174649242109 0.0 30-31 6.900268351436188E-5 0.0 0.0 0.04543826709420729 0.0 32-33 1.0350402527154281E-4 0.0 0.0 0.06631157885730177 0.0 34-35 1.3800536702872376E-4 0.0 0.0 0.09981238170352444 0.0 36-37 1.3800536702872376E-4 0.0 0.0 0.14528515013948892 0.0 38-39 2.0700805054308562E-4 0.0 0.0 0.21601290074170987 0.0 40-41 2.0700805054308562E-4 0.0 0.0 0.34694549271021147 0.0 42-43 2.760107340574475E-4 0.0 0.0 0.4321983081922056 0.0 44-45 2.760107340574475E-4 0.0 0.0 0.5202457323565314 0.0 46-47 2.760107340574475E-4 0.0 0.0 0.6375502943309466 0.0 48-49 3.4501341757180935E-4 0.0 0.0 0.7367761532245989 0.0 50-51 3.4501341757180935E-4 0.0 0.0 0.8405906905719563 0.0 52-53 3.4501341757180935E-4 0.0 0.0 0.9817356797005836 0.0 54-55 3.4501341757180935E-4 0.0 0.0 1.1212246044248662 0.0 56-57 3.4501341757180935E-4 0.0 0.0 1.278171208078282 0.0 58-59 3.4501341757180935E-4 0.0 0.0 1.4629603945297434 0.0 60-61 3.4501341757180935E-4 0.0 0.0 1.6203210142842455 0.0 62-63 3.4501341757180935E-4 6.900268351436188E-5 0.0 1.776405084393732 0.0 64-65 3.4501341757180935E-4 6.900268351436188E-5 0.0 1.9658864533241698 0.0 66-67 4.1401610108617124E-4 6.900268351436188E-5 0.0 2.175516605840801 0.0 68-69 4.1401610108617124E-4 6.900268351436188E-5 0.0 2.4402799024854076 0.0 70-71 4.485174428433522E-4 6.900268351436188E-5 0.0 2.858746676658255 0.0 72-73 4.8301878460053313E-4 6.900268351436188E-5 0.0 3.1648080793862072 0.0 74-75 4.8301878460053313E-4 6.900268351436188E-5 0.0 3.4657287821923397 0.0 76-77 4.8301878460053313E-4 6.900268351436188E-5 0.0 3.8204025754561597 0.0 78-79 4.8301878460053313E-4 6.900268351436188E-5 0.0 4.134744300205835 0.0 80-81 4.8301878460053313E-4 6.900268351436188E-5 0.0 4.464404620695699 0.0 82-83 4.8301878460053313E-4 6.900268351436188E-5 0.0 4.895981904736275 0.0 84-85 5.17520126357714E-4 6.900268351436188E-5 0.0 5.2981985469414905 0.0 86-87 5.52021468114895E-4 6.900268351436188E-5 0.0 5.709523543370602 0.0 88-89 5.52021468114895E-4 6.900268351436188E-5 0.0 6.207515910293751 0.0 90-91 6.900268351436187E-4 6.900268351436188E-5 0.0 6.63771314066404 0.0 92-93 6.900268351436187E-4 6.900268351436188E-5 0.0 7.074155113892379 0.0 94-95 6.900268351436187E-4 6.900268351436188E-5 0.0 7.579185754533993 0.0 96-97 6.900268351436187E-4 6.900268351436188E-5 0.0 8.073279469838582 0.0 98-99 7.590295186579806E-4 6.900268351436188E-5 0.0 8.65673166029427 0.0 100-101 7.590295186579806E-4 6.900268351436188E-5 0.0 9.574915868478126 0.0 102-103 7.935308604151616E-4 6.900268351436188E-5 0.0 10.276052135667555 0.0 104-105 8.970348856867043E-4 6.900268351436188E-5 0.0 10.938546900088944 0.0 106-107 9.315362274438853E-4 6.900268351436188E-5 0.0 11.634990984799398 0.0 108-109 9.660375692010663E-4 6.900268351436188E-5 0.0 12.227758537529525 0.0 110-111 9.660375692010663E-4 6.900268351436188E-5 0.0 12.848989697209324 0.0 112-113 9.660375692010663E-4 6.900268351436188E-5 0.0 13.650076351469309 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 270 0.0 66.10528 2 ATAACGC 275 0.0 64.903366 3 TACCGTA 50 7.453811E-6 59.498863 7 AGTACTC 1640 0.0 57.318115 5 TAACGCA 365 0.0 52.159786 4 AAAGTAC 1740 0.0 51.28858 3 AAGTACT 1885 0.0 49.868286 4 GTATAAC 365 0.0 48.979225 1 TATCACG 130 1.8189894E-11 45.765198 2 AAAAAGT 2055 0.0 42.627415 1 GTACCGT 85 3.4819768E-6 41.999195 6 GTATACG 60 0.0012987874 39.727592 1 AAAAGTA 2260 0.0 38.961166 2 ACCGTAT 65 0.0019392485 36.61468 8 AGCGTCA 125 1.2792061E-6 33.317062 3 ATCACGC 180 6.039045E-10 33.052643 3 GATCGGA 75 0.0039111874 31.732723 9 CCGTATG 75 0.0039111874 31.732723 9 GTTATCA 780 0.0 31.323679 1 TCACGCA 190 1.0768417E-9 31.31303 4 >>END_MODULE