##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1230240 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.26750390167772 31.0 30.0 34.0 26.0 34.0 2 30.260264663805437 31.0 30.0 34.0 26.0 34.0 3 30.428401775263364 31.0 31.0 34.0 26.0 34.0 4 33.72489107816361 37.0 35.0 37.0 28.0 37.0 5 33.76345265964365 37.0 35.0 37.0 30.0 37.0 6 33.648149954480424 37.0 35.0 37.0 28.0 37.0 7 33.56349248927039 37.0 35.0 37.0 28.0 37.0 8 33.39184793211081 37.0 35.0 37.0 26.0 37.0 9 34.71798429574717 39.0 35.0 39.0 25.0 39.0 10-11 34.716889387436595 39.0 35.0 39.0 26.0 39.0 12-13 34.59265671738848 39.0 35.0 39.0 25.0 39.0 14-15 35.718822750032516 40.0 36.0 41.0 25.0 41.0 16-17 35.75065353101834 40.0 36.0 41.0 25.0 41.0 18-19 35.66267557549746 40.0 36.0 41.0 25.0 41.0 20-21 35.68824131876707 40.0 36.0 41.0 25.0 41.0 22-23 35.706625536480686 40.0 36.0 41.0 25.5 41.0 24-25 35.725386509949274 40.0 36.0 41.0 25.5 41.0 26-27 35.5126077025621 40.0 35.0 41.0 25.0 41.0 28-29 35.374982117310445 40.0 35.0 41.0 25.0 41.0 30-31 35.40605572896345 40.0 35.0 41.0 25.0 41.0 32-33 35.022076993107035 39.0 34.5 41.0 24.5 41.0 34-35 35.06562134217714 39.0 35.0 41.0 25.0 41.0 36-37 35.06506982377422 39.0 34.5 41.0 25.0 41.0 38-39 34.87417698985564 39.0 34.0 41.0 24.5 41.0 40-41 34.43832179087008 38.5 33.0 41.0 23.5 41.0 42-43 34.13913220184679 38.0 33.0 40.5 21.5 41.0 44-45 33.823574668357395 38.0 33.0 40.0 18.5 41.0 46-47 33.48387509754194 38.0 33.0 40.0 16.0 41.0 48-49 33.10343875991676 38.0 31.5 40.0 14.0 41.0 50-51 32.93470176550917 38.0 31.5 40.0 11.0 41.0 52-53 32.7317320197685 38.0 31.0 40.0 8.5 41.0 54-55 32.338858677981534 37.0 31.0 40.0 6.5 41.0 56-57 31.816310638574585 37.0 30.0 40.0 2.0 41.0 58-59 31.36095883730004 36.0 29.0 40.0 2.0 41.0 60-61 31.010714169592923 36.0 29.0 39.0 2.0 41.0 62-63 30.330169722980884 35.0 28.0 39.0 2.0 41.0 64-65 30.031373146703082 35.0 28.0 39.0 2.0 40.5 66-67 29.540370984523346 34.5 27.5 38.0 2.0 40.0 68-69 28.998993285862923 34.0 26.0 37.0 2.0 40.0 70-71 28.232239644297046 34.0 26.0 37.0 2.0 39.5 72-73 27.880697262322798 33.0 26.0 36.0 2.0 39.0 74-75 27.399442791650408 33.0 25.5 36.0 2.0 39.0 76-77 26.771857523735207 33.0 23.0 35.0 2.0 38.0 78-79 26.30982369293796 32.5 21.0 35.0 2.0 37.0 80-81 26.0347797990636 32.0 20.0 35.0 2.0 37.0 82-83 25.795790252308493 32.0 20.0 35.0 2.0 36.0 84-85 25.480419674209912 32.0 20.0 35.0 2.0 36.0 86-87 25.007387582910653 32.0 18.0 35.0 2.0 36.0 88-89 24.646355182728573 32.0 13.0 35.0 2.0 35.0 90-91 24.446182452204447 32.0 10.0 35.0 2.0 35.0 92-93 24.00769809142931 31.0 7.0 35.0 2.0 35.0 94-95 23.89785245155417 31.0 7.0 35.0 2.0 35.0 96-97 23.77129462543894 31.0 2.0 35.0 2.0 35.0 98-99 23.473304802314995 31.0 2.0 35.0 2.0 35.0 100-101 22.840396182858626 31.0 2.0 35.0 2.0 35.0 102-103 21.574266809728183 28.0 2.0 34.0 2.0 35.0 104-105 22.186660732865132 30.0 2.0 35.0 2.0 35.0 106-107 22.178504600728314 30.0 2.0 35.0 2.0 35.0 108-109 22.083637339055795 30.0 2.0 35.0 2.0 35.0 110-111 22.14884819222266 30.5 2.0 35.0 2.0 35.0 112-113 22.022908538171414 30.5 2.0 35.0 2.0 35.0 114-115 21.824986587982835 30.5 2.0 35.0 2.0 35.0 116-117 21.40666902393029 30.0 2.0 35.0 2.0 35.0 118-119 21.12420340746521 30.0 2.0 35.0 2.0 35.0 120-121 20.882644443360647 30.0 2.0 35.0 2.0 35.0 122-123 20.406564979191053 29.0 2.0 35.0 2.0 35.0 124-125 19.41636469306802 27.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34905428097932756 1101 2 0.4966315747692427 1101 3 0.4843870242388242 1101 4 0.4162775033362536 1101 5 0.4497124419247882 1101 6 0.4674875652985051 1101 7 0.4597343316012754 1101 8 0.45853471291454895 1101 9 0.7193986461339676 1101 10-11 0.6657188484216761 1101 12-13 0.6758580687089761 1101 14-15 0.8105494953177086 1101 16-17 0.6467507442676066 1101 18-19 0.636199723568275 1101 20-21 0.6992737822995849 1101 22-23 0.7262268353895607 1101 24-25 0.8503251039225361 1101 26-27 0.8869621869421067 1101 28-29 0.9921698857524746 1101 30-31 0.854838591276021 1101 32-33 0.8875521049513964 1101 34-35 0.7162421221863227 1101 36-37 0.7913828818080617 1101 38-39 0.6310917707246375 1101 40-41 0.7650838657729224 1101 42-43 0.69438037013132 1101 44-45 0.740609693436852 1101 46-47 0.9532759337215069 1101 48-49 0.9923438207329127 1101 50-51 0.9849790817518453 1101 52-53 0.9302764183443202 1101 54-55 0.8530435729379917 1101 56-57 1.1617082731672248 1101 58-59 1.0662870942616003 1101 60-61 0.9882382470388471 1101 62-63 1.1008260777591232 1101 64-65 0.9919587519621906 1101 66-67 1.079114966080386 1101 68-69 0.8903779168299906 1101 70-71 1.0062150281161202 1101 72-73 0.8489261984947376 1101 74-75 0.8339037448203221 1101 76-77 0.909759995719245 1101 78-79 0.9647654637913696 1101 80-81 1.0927536408545926 1101 82-83 0.8165269258384313 1101 84-85 0.9117634915287098 1101 86-87 0.951238742619978 1101 88-89 0.9303895028658893 1101 90-91 0.7183696864521565 1101 92-93 0.9068787636088231 1101 94-95 0.7212104579352392 1101 96-97 0.7529675214164548 1101 98-99 0.5663970227358988 1101 100-101 0.7691550642607581 1101 102-103 0.6771847681857786 1101 104-105 0.5719716148052925 1101 106-107 0.4664390403900107 1101 108-109 0.4954765066430973 1101 110-111 0.43020649239726083 1101 112-113 0.3976370886880325 1101 114-115 0.5947383823270762 1101 116-117 0.5661047832835884 1101 118-119 0.368009421718714 1101 120-121 0.2251907688669732 1101 122-123 0.32828063213782244 1101 124-125 0.3667647370825904 1102 1 0.4030205717753468 1102 2 0.5163685073256268 1102 3 0.5209247102168852 1102 4 0.589637814420584 1102 5 0.5086525646078641 1102 6 0.5179757541305392 1102 7 0.6004134307949229 1102 8 0.5511785974357224 1102 9 0.6357245582943492 1102 10-11 0.6496324478204087 1102 12-13 0.5719323361965891 1102 14-15 0.6615251569103862 1102 16-17 0.5892986179365707 1102 18-19 0.6005526871872675 1102 20-21 0.775115194827741 1102 22-23 0.7312307422750806 1102 24-25 0.7803116532375114 1102 26-27 0.8004859685323069 1102 28-29 0.8687823353124102 1102 30-31 0.7535411271998882 1102 32-33 0.8773618361897562 1102 34-35 0.8232512581115685 1102 36-37 0.6770768351866536 1102 38-39 0.6508229747098824 1102 40-41 0.8461126426771557 1102 42-43 0.896752743295032 1102 44-45 0.8996322502585485 1102 46-47 0.6674196612798298 1102 48-49 0.7778210049798062 1102 50-51 0.846273332099571 1102 52-53 0.9445341158253484 1102 54-55 1.0659112612762272 1102 56-57 1.0154259153331715 1102 58-59 0.9517500430092234 1102 60-61 0.8646672391082149 1102 62-63 0.8921276434921843 1102 64-65 0.9585951240351775 1102 66-67 0.9713518069796301 1102 68-69 0.921820468427228 1102 70-71 0.8714276211194729 1102 72-73 0.8833700917233358 1102 74-75 0.7546884903228239 1102 76-77 0.7741744555493568 1102 78-79 0.8650001060650183 1102 80-81 0.8681652959762793 1102 82-83 1.0821066343229013 1102 84-85 1.0070678676987725 1102 86-87 1.0264223719676764 1102 88-89 1.007455683902485 1102 90-91 0.8430483136115399 1102 92-93 0.945584427332534 1102 94-95 1.0613248918722142 1102 96-97 1.2224024437026593 1102 98-99 1.1912655520283728 1102 100-101 0.9331659176116318 1102 102-103 0.7335753902856901 1102 104-105 0.6755854266194419 1102 106-107 0.6485715124517704 1102 108-109 0.7833048396976423 1102 110-111 0.7089739444892196 1102 112-113 0.6664298095072674 1102 114-115 0.5083860442680503 1102 116-117 0.41029996486783915 1102 118-119 0.3171574686196692 1102 120-121 0.42918618944720777 1102 122-123 0.4422617255616501 1102 124-125 0.3055602534445363 1103 1 0.43643912385150685 1103 2 0.373399715930514 1103 3 0.37947484356434913 1103 4 0.34682179859550644 1103 5 0.2615910658177043 1103 6 0.276254777073369 1103 7 0.34616353235094266 1103 8 0.3486317492045643 1103 9 0.45561055062908196 1103 10-11 0.5177100807704704 1103 12-13 0.43485710526704224 1103 14-15 0.5043639124518151 1103 16-17 0.42098166602996656 1103 18-19 0.48437481403514226 1103 20-21 0.47058403037764407 1103 22-23 0.4609526554137773 1103 24-25 0.5319586268682741 1103 26-27 0.6122198829426679 1103 28-29 0.6191190155787538 1103 30-31 0.5543191345825704 1103 32-33 0.6168971511900168 1103 34-35 0.48864626685421086 1103 36-37 0.5140463153964845 1103 38-39 0.6335319593263549 1103 40-41 0.6883707627428635 1103 42-43 0.8121919068267047 1103 44-45 0.9019790772954153 1103 46-47 0.8324404581854594 1103 48-49 0.7811988463404731 1103 50-51 0.8950941784585638 1103 52-53 1.028531978733561 1103 54-55 1.1099159240219407 1103 56-57 1.139737100624334 1103 58-59 1.184273577511778 1103 60-61 1.1315785233061 1103 62-63 1.0704754595963202 1103 64-65 0.727811288220348 1103 66-67 0.8363341892188068 1103 68-69 0.976527148163715 1103 70-71 1.0770200459238666 1103 72-73 0.8970547647129692 1103 74-75 0.9663433676608157 1103 76-77 0.9650545046494869 1103 78-79 1.002279552893821 1103 80-81 1.0154171019071505 1103 82-83 0.9455060673754119 1103 84-85 1.0983225701838037 1103 86-87 0.9992152948380948 1103 88-89 1.02236786002711 1103 90-91 0.8741785437463037 1103 92-93 0.8586127393832612 1103 94-95 0.864094280240078 1103 96-97 0.8847750571363129 1103 98-99 0.8369388242514795 1103 100-101 0.8300059801348105 1103 102-103 0.6199739490733727 1103 104-105 0.9009801704333569 1103 106-107 0.7900478719777233 1103 108-109 0.7834936014207372 1103 110-111 1.060882093697085 1103 112-113 0.819499008892631 1103 114-115 1.0397094516048107 1103 116-117 0.9340364755797914 1103 118-119 1.0026373870216219 1103 120-121 0.9029416867184423 1103 122-123 0.7665400155349786 1103 124-125 0.6748377927864517 1104 1 0.5561318104489992 1104 2 0.6130896002441979 1104 3 0.49959094296392337 1104 4 0.5708147069195135 1104 5 0.4823845140558376 1104 6 0.7158326258572174 1104 7 0.486729883956059 1104 8 0.5744475375618023 1104 9 0.841177449293582 1104 10-11 0.7333892215076432 1104 12-13 0.7613752229808313 1104 14-15 0.9615146485258066 1104 16-17 0.8865033876370276 1104 18-19 0.844291465629766 1104 20-21 0.7856698628792245 1104 22-23 0.8367844089735641 1104 24-25 0.9022240511519044 1104 26-27 0.9680949937501069 1104 28-29 0.9617947835273171 1104 30-31 1.0188493110482995 1104 32-33 0.9364856173917957 1104 34-35 0.8460396070202094 1104 36-37 0.8479636904492338 1104 38-39 0.8688905846665946 1104 40-41 0.8706863981467592 1104 42-43 0.9434708088673531 1104 44-45 0.9521634028158417 1104 46-47 0.9292461058479589 1104 48-49 0.9294562668103268 1104 50-51 0.8249184607501405 1104 52-53 0.8055275453196202 1104 54-55 0.9684991183575349 1104 56-57 1.0210493565409458 1104 58-59 0.9106584751000248 1104 60-61 0.9623992910202155 1104 62-63 0.6988295476929771 1104 64-65 0.7775622924650811 1104 66-67 0.730551883129035 1104 68-69 0.7424468554757766 1104 70-71 0.7928966583535022 1104 72-73 0.8297607381549099 1104 74-75 0.8722339412693323 1104 76-77 0.9842193068484377 1104 78-79 0.9521725413787792 1104 80-81 0.7725643211707869 1104 82-83 0.6612376811559457 1104 84-85 0.8292844848023471 1104 86-87 0.932324445466989 1104 88-89 0.7269193662267419 1104 90-91 0.8594457599285406 1104 92-93 0.7050291654554357 1104 94-95 0.7068738778276575 1104 96-97 0.7306790336998432 1104 98-99 0.6337779932571053 1104 100-101 0.7633063224376855 1104 102-103 0.3393517415882634 1104 104-105 0.44118149945273544 1104 106-107 0.6843716284224151 1104 108-109 0.6246355097425145 1104 110-111 0.5571237119657404 1104 112-113 0.32254146851629883 1104 114-115 0.46865634553557456 1104 116-117 0.35635585666582514 1104 118-119 0.20760421177728716 1104 120-121 0.22835131139104448 1104 122-123 0.30053738406768105 1104 124-125 0.2709333796918614 1105 1 0.4021172713879615 1105 2 0.5566810025135247 1105 3 0.5790799315956008 1105 4 0.5793162669803991 1105 5 0.3968380004908596 1105 6 0.4123261807852927 1105 7 0.5151124516558028 1105 8 0.5330446702981035 1105 9 0.524657657420363 1105 10-11 0.44021249590842615 1105 12-13 0.5324541220970787 1105 14-15 0.5661134004214716 1105 16-17 0.5470343339664296 1105 18-19 0.5769306258695295 1105 20-21 0.5751360381060877 1105 22-23 0.6697584698847763 1105 24-25 0.6730881099021886 1105 26-27 0.7061973567716464 1105 28-29 0.9044976227296644 1105 30-31 0.798761417003945 1105 32-33 0.4914067410806098 1105 34-35 0.6553176794251669 1105 36-37 0.6773777978271482 1105 38-39 0.6866610394857346 1105 40-41 0.8669156368498392 1105 42-43 0.7830059137831498 1105 44-45 0.8335758315187363 1105 46-47 0.8852319199872554 1105 48-49 0.8401370451748846 1105 50-51 0.9209496013397285 1105 52-53 0.9570173972063145 1105 54-55 0.9557396864483039 1105 56-57 0.9555623975597491 1105 58-59 1.0420129872900254 1105 60-61 0.9728180058548794 1105 62-63 0.9187589187488499 1105 64-65 0.887983844026909 1105 66-67 1.0781127010297844 1105 68-69 1.0559627757726417 1105 70-71 1.2044615500169478 1105 72-73 1.014603826816817 1105 74-75 0.9215661562276161 1105 76-77 0.890029535919723 1105 78-79 0.8273194184898109 1105 80-81 0.8579936111574469 1105 82-83 0.8815269272067106 1105 84-85 0.8826619561270661 1105 86-87 0.7720657693230564 1105 88-89 0.5511932961757111 1105 90-91 0.6146750022470613 1105 92-93 0.7055578535275302 1105 94-95 0.47718074217897666 1105 96-97 0.4744447000122882 1105 98-99 0.5190489271215881 1105 100-101 0.504227335438518 1105 102-103 0.33147912843691785 1105 104-105 0.4752622733119054 1105 106-107 0.412472164257931 1105 108-109 0.4129013600250637 1105 110-111 0.4406821803889365 1105 112-113 0.5105244247920417 1105 114-115 0.6304430998552597 1105 116-117 0.5507151915701165 1105 118-119 0.44711228435188843 1105 120-121 0.48139543946315655 1105 122-123 0.6541853456706299 1105 124-125 0.7908573554039151 1106 1 0.2662803965902114 1106 2 0.12813126282157938 1106 3 0.17807441926402845 1106 4 0.22908048792938018 1106 5 0.049852377619622246 1106 6 0.16130731770336837 1106 7 0.1808212830319107 1106 8 0.28097820907552773 1106 9 0.24582578365960472 1106 10-11 0.21356222569783512 1106 12-13 0.23743411618080756 1106 14-15 0.2675284789130217 1106 16-17 0.25636462647796066 1106 18-19 0.33345550512544975 1106 20-21 0.27596896325422904 1106 22-23 0.26622705116103873 1106 24-25 0.44984272311062057 1106 26-27 0.4804346525285297 1106 28-29 0.5231717732266006 1106 30-31 0.43501940250834537 1106 32-33 0.1715544658070911 1106 34-35 0.3713790489216464 1106 36-37 0.4183676833390919 1106 38-39 0.3525463230685517 1106 40-41 0.3818150489873773 1106 42-43 0.5829051958234928 1106 44-45 0.5546825720940873 1106 46-47 0.40546531687032683 1106 48-49 0.39948485659913047 1106 50-51 0.4633708843304234 1106 52-53 0.4435062357516557 1106 54-55 0.5737041125719173 1106 56-57 0.5368284276174968 1106 58-59 0.7731783878037 1106 60-61 0.6352713761172915 1106 62-63 0.6161409843840175 1106 64-65 0.772781772778611 1106 66-67 0.760036189230977 1106 68-69 0.7893981827709631 1106 70-71 0.8046664467740889 1106 72-73 0.7666068814482898 1106 74-75 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2216 82-83 -2.969163883448207 2216 84-85 -3.0780542825277735 2216 86-87 -3.1091699086879316 2216 88-89 -3.139631778887569 2216 90-91 -3.0549961470385725 2216 92-93 -2.982381907383594 2216 94-95 -2.801016853797087 2216 96-97 -2.864080932867928 2216 98-99 -2.8125020666549005 2216 100-101 -2.7272939614037597 2216 102-103 -2.5933045336805023 2216 104-105 -2.7142791232149825 2216 106-107 -2.634513953853059 2216 108-109 -2.5245212030773523 2216 110-111 -2.6666949378377005 2216 112-113 -2.6718577262083016 2216 114-115 -2.8894732632982674 2216 116-117 -2.687641682770451 2216 118-119 -2.583149177165595 2216 120-121 -2.661915254716959 2216 122-123 -2.434304379618453 2216 124-125 -2.565772699321254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42175.0 3 7713.0 4 5076.0 5 3832.0 6 4471.0 7 4495.0 8 5268.0 9 6498.0 10 8016.0 11 9691.0 12 11243.0 13 12681.0 14 13873.0 15 14208.0 16 14731.0 17 15332.0 18 15960.0 19 17443.0 20 18497.0 21 18985.0 22 19912.0 23 21781.0 24 24124.0 25 25547.0 26 29058.0 27 30698.0 28 34095.0 29 40528.0 30 45633.0 31 51473.0 32 59971.0 33 67838.0 34 74115.0 35 83531.0 36 117194.0 37 178554.0 38 75567.0 39 433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.004792192654804 16.679966968421517 9.459495884529966 22.855744954393714 2 12.230873895196606 23.813770697453336 35.98012456448981 27.975230842860242 3 23.168445224026364 32.33414013529448 26.083548099442428 18.413866541236725 4 13.718004728093778 19.453696936538204 31.52225837930091 35.30603995606711 5 10.960896464316514 42.729293516607 32.273483027151336 14.036326991925158 6 31.206318406253725 27.702562544610554 21.56815186127077 19.52296718786495 7 25.097209543111067 33.49528119319917 21.846303587730286 19.56120567595948 8 30.153093228091265 32.46370324873892 21.942872637934187 15.440330885235618 9 32.54307316802211 15.843214648634696 18.746998029727266 32.86671415361592 10-11 23.55439446926457 30.958770272378754 23.41000750730512 22.076827751051553 12-13 27.700065719526705 27.87100184088196 22.79025749882673 21.63867494076461 14-15 29.114413550263652 25.543418118442712 22.20730394132542 23.134864389968218 16-17 23.541770542251996 28.940769171400532 29.529618681148605 17.987841605198863 18-19 26.24211061833807 27.81921118024088 28.044062130233083 17.894616071187965 20-21 21.913737660402013 28.806517145278033 29.009953346876692 20.269791847443265 22-23 20.526821570303525 31.369477152237685 21.634055983725766 26.469645293733024 24-25 21.28706624605678 33.18803916163874 20.508121241820056 25.016773350484417 26-27 24.507281428497915 26.77808734773691 21.787762429968986 26.92686879379618 28-29 23.843464184144025 27.104225439540784 25.542575383102488 23.509734993212703 30-31 27.271301460362324 29.147573993256724 23.84346288237252 19.737661664008428 32-33 19.39093832680804 29.737561580479184 27.164353203818624 23.707146888894155 34-35 16.918133990480943 31.511148175949806 28.093468737977638 23.477249095591617 36-37 24.847739639375945 28.357270021436264 24.548178937918923 22.246811401268868 38-39 28.01784872653376 26.25505234443064 23.592556093520145 22.134542835515454 40-41 22.236231550530693 31.024283198435743 23.734876046007177 23.004609205026384 42-43 26.193465875586462 28.77372638685585 22.874332429160056 22.15847530839763 44-45 28.388859769011333 26.114080250028167 23.204718726476173 22.292341254484324 46-47 23.360687695272873 28.08740876039057 30.09475161229611 18.457151932040446 48-49 25.951028493092075 27.464435686215914 28.022466570810607 18.562069249881404 50-51 20.81101909663262 29.019501554377076 29.109466025758245 21.06001332323206 52-53 19.79119302991738 31.30937210971039 22.08252687535663 26.816907985015597 54-55 20.731208058211255 32.68411529433182 20.935006222517554 25.649670424939373 56-57 24.040840245736863 26.65344719652828 22.014906795797955 27.290805761936905 58-59 23.57142070073325 26.767127055701728 25.525492803181955 24.13595944038307 60-61 26.438182449698765 28.915316953792292 24.124470580456464 20.52203001605248 62-63 19.169806084007135 29.552168748954095 27.014567168565286 24.263457998473488 64-65 16.670782879114395 30.957278613425604 28.12827723650151 24.243661270958494 66-67 24.057308831104585 28.263836790405044 24.66038137858915 23.018472999901224 68-69 27.033448684886167 26.29897032615999 23.735480031446155 22.932100957507686 70-71 21.67948354733809 30.319590600065155 23.914266376119862 24.086659476476893 72-73 25.381037447318533 28.254575796814862 23.34966778629245 23.014718969574155 74-75 27.38255959639423 25.646831071732855 23.58948838527074 23.38112094660217 76-77 22.559147413490823 27.599622658299623 30.02952523278725 19.811704695422304 78-79 25.008581650166633 27.11385125275766 28.036342931312102 19.841224165763606 80-81 20.2023220252776 28.57188342685277 29.07470860387026 22.151085943999366 82-83 19.2573002332009 30.526132746949873 22.572239010310557 27.644328009538665 84-85 20.187799176358073 31.862672464570295 21.564740059701588 26.384788299370044 86-87 23.108292631153546 26.049708026068448 22.7658024129436 28.076196929834406 88-89 22.84453079241725 26.031832198315534 26.017342609391292 25.10629439987592 90-91 25.38728831442999 28.217504333816752 24.63342665614659 21.76178069560667 92-93 18.92113763755407 28.652273180951088 27.114271986505955 25.312317194988882 94-95 16.719774603547823 29.832332405883427 28.342044504532748 25.105848486036002 96-97 23.48634014024071 27.308089243791372 25.3152722647328 23.890298351235113 98-99 25.911277195530406 25.801699554917327 24.383064828677846 23.903958420874417 100-101 21.142985124312187 28.775342193037996 24.68452804448999 25.39714463815983 102-103 24.241371152606806 27.3887488477195 24.334491732132516 24.035388267541176 104-105 25.685795199382618 25.07175800065507 24.618633300199093 24.62381349976322 106-107 21.528773324347235 26.957433805728964 30.19304061612422 21.320752253799576 108-109 23.417810348583966 26.927028211319286 28.53086207191997 21.124299368176768 110-111 19.189664093653544 28.395993738584263 29.488966621561403 22.925375546200794 112-113 18.50848038585603 29.98112749448617 23.389869239040177 28.12052288061763 114-115 19.140943628506104 31.76000708372217 22.629187138800557 26.469862148971167 116-117 21.666725816998664 26.603453318761954 23.60086008662056 28.128960777618822 118-119 21.280430503290745 26.711770666043975 26.240550490325354 25.767248340339926 120-121 23.370272370109472 28.518044167618278 25.28257485350201 22.82910860877024 122-123 18.214304223151988 28.97150573154006 26.950704797224144 25.863485248083805 124-125 16.585873819526732 30.18163700997914 27.832891012897477 25.39959815759665 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6107.0 1 3711.0 2 2117.5 3 2911.0 4 2611.5 5 2074.5 6 1635.0 7 1317.5 8 1174.5 9 1122.0 10 1041.5 11 942.5 12 921.0 13 912.5 14 888.0 15 925.0 16 1024.5 17 1133.0 18 1249.0 19 1421.0 20 1557.5 21 1782.0 22 2222.5 23 2771.0 24 3343.0 25 4060.5 26 5080.0 27 6166.5 28 6914.0 29 7812.0 30 8986.0 31 10177.0 32 11354.5 33 12575.0 34 13944.5 35 14906.5 36 15874.0 37 17197.0 38 17802.0 39 17751.5 40 17702.0 41 18623.0 42 19676.0 43 20709.5 44 22316.5 45 24054.5 46 27959.0 47 36927.5 48 50458.5 49 85188.0 50 189125.5 51 203951.5 52 98660.5 53 43943.0 54 28292.0 55 21317.0 56 17163.5 57 14523.5 58 12895.5 59 11526.5 60 10227.0 61 8960.0 62 7555.5 63 5797.0 64 4256.5 65 3298.0 66 2386.5 67 1820.0 68 1391.5 69 926.5 70 743.5 71 698.5 72 674.0 73 599.0 74 431.5 75 356.0 76 261.0 77 127.5 78 77.5 79 54.0 80 32.5 81 16.0 82 10.5 83 8.5 84 6.5 85 10.0 86 8.0 87 2.0 88 3.0 89 4.5 90 3.5 91 2.5 92 3.5 93 3.0 94 2.0 95 1.5 96 1.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4842144622187541 2 0.472590714007023 3 0.4594225516972298 4 0.45885355702952274 5 0.46299908960853164 6 0.46771361685524776 7 0.45243204577968527 8 0.4786058004942125 9 0.4912862530888282 10-11 0.4957569254779555 12-13 0.4957162830016907 14-15 0.47076180257510736 16-17 0.4729564962934062 18-19 0.4593412667447002 20-21 0.46962381323969304 22-23 0.48555566393549227 24-25 0.4736880608661725 26-27 0.48453960202887236 28-29 0.47909351020939006 30-31 0.49238359994797765 32-33 0.4900669788008844 34-35 0.4843770321238132 36-37 0.4427184939523996 38-39 0.4017508778774873 40-41 0.3955325790089738 42-43 0.4060589803615555 44-45 0.4404018728053063 46-47 0.44800201586682276 48-49 0.4460105345298478 50-51 0.43178566783717 52-53 0.42536415658733256 54-55 0.4270304981141891 56-57 0.40861945636623753 58-59 0.41296820132657047 60-61 0.42231597086747297 62-63 0.42471387696709584 64-65 0.43962966575627516 66-67 0.4278027051632202 68-69 0.4299161139289895 70-71 0.4408082975679542 72-73 0.44105215242554296 74-75 0.4454821823384055 76-77 0.43532156327220706 78-79 0.4261363636363636 80-81 0.4357686305111198 82-83 0.43328943945896736 84-85 0.4319888802184939 86-87 0.4379226817531539 88-89 0.4245106645857719 90-91 0.42922519183248803 92-93 0.43922324099362725 94-95 0.4369879047990636 96-97 0.40557127064637793 98-99 0.3759429054493432 100-101 0.3752113408765769 102-103 0.3584666406554819 104-105 0.35915756275198335 106-107 0.3774060345948758 108-109 0.3852093900377162 110-111 0.4030107946416959 112-113 0.3993123293015997 114-115 0.3979304851085967 116-117 0.36980589153335935 118-119 0.44227142671348685 120-121 0.45231011835089086 122-123 0.40305143711796065 124-125 0.39699570815450647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1230240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.58608401398137 #Duplication Level Percentage of deduplicated Percentage of total 1 87.9398540685262 45.364727001562535 2 8.439923028754366 8.707651568657177 3 1.8193679051378242 2.8156219682034322 4 0.6933664637561971 1.4307224260721743 5 0.3120317236141374 0.8048247354693153 6 0.18477585911900074 0.5719117795361014 7 0.11396482064574516 0.411529916872881 8 0.08283883250538282 0.34186647785942476 9 0.06217274834882492 0.2886523757732343 >10 0.3024471169724451 2.8364581076150497 >50 0.022795749604942253 0.8012660093950877 >100 0.018354924946370206 1.992664080307906 >500 0.002753238589335638 1.0153480900623826 >1k 0.002294365491113032 3.350222610814322 >5k 0.001376619294667819 4.928399262146131 >10k+ 0.00168253469348289 24.33813358965277 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 75806 6.161887111457927 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 56053 4.556265444140981 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 36526 2.969014176095721 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 24069 1.956447522434647 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 20227 1.6441507348159707 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 19829 1.611799323709195 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 19254 1.565060476004682 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16652 1.3535570295226946 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 14947 1.2149661854597478 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12941 1.0519085706853948 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 12145 0.9872057484718428 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8458 0.6875081284952529 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8300 0.674665105995578 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7842 0.6374365977370269 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7190 0.5844388086877358 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6992 0.5683443880868774 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6673 0.5424144882299389 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 6219 0.5055111197815061 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5653 0.45950383664975936 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 5133 0.4172356613343738 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4969 0.4039049291195214 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4899 0.39821498244245024 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4785 0.38894849785407726 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4545 0.3694401092469762 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4091 0.3325367407985434 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3674 0.2986409155937053 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3022 0.2456431265444141 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2382 0.19362075692547795 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2187 0.17777019118220835 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1750 0.1422486669267785 No Hit ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 1440 0.11705033164260631 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 1337 0.10867798153205879 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.4385485758876316E-4 0.0 6 0.0 0.0 0.0 2.4385485758876316E-4 0.0 7 0.0 0.0 0.0 2.4385485758876316E-4 0.0 8 0.0 0.0 0.0 2.4385485758876316E-4 0.0 9 0.0 0.0 0.0 2.4385485758876316E-4 0.0 10-11 0.0 0.0 0.0 4.877097151775264E-4 0.0 12-13 0.0 0.0 0.0 0.0012599167642086098 0.0 14-15 8.128495252958772E-5 0.0 0.0 0.0030888281961243334 0.0 16-17 8.128495252958772E-5 0.0 0.0 0.00536480686695279 0.0 18-19 8.128495252958772E-5 0.0 0.0 0.007437573156457277 0.0 20-21 8.128495252958772E-5 0.0 0.0 0.01032318897125764 0.0 22-23 8.128495252958772E-5 0.0 0.0 0.014550006502796203 0.0 24-25 8.128495252958772E-5 0.0 0.0 0.019630316035895434 0.0 26-27 8.128495252958772E-5 0.0 0.0 0.02462934061646508 0.0 28-29 1.6256990505917544E-4 0.0 0.0 0.03157920405774483 0.0 30-31 2.4385485758876316E-4 0.0 0.0 0.04214624788659123 0.0 32-33 2.4385485758876316E-4 0.0 0.0 0.06336162049681363 0.0 34-35 2.4385485758876316E-4 0.0 0.0 0.09445311483938093 0.0 36-37 2.4385485758876316E-4 0.0 0.0 0.13424209910261412 0.0 38-39 2.4385485758876316E-4 0.0 0.0 0.19898556379243074 0.0 40-41 2.4385485758876316E-4 0.0 0.0 0.3085170373260502 0.0 42-43 2.4385485758876316E-4 0.0 0.0 0.3813483547925608 0.0 44-45 2.4385485758876316E-4 0.0 0.0 0.458000065027962 0.0 46-47 3.657822863831448E-4 0.0 0.0 0.560337820262713 0.0 48-49 4.064247626479386E-4 0.0 0.0 0.6520272467160879 0.0 50-51 4.064247626479386E-4 0.0 0.0 0.7504226817531539 0.0 52-53 4.064247626479386E-4 0.0 0.0 0.88230751723241 0.0 54-55 4.064247626479386E-4 0.0 0.0 1.0133388607101055 0.0 56-57 4.064247626479386E-4 0.0 0.0 1.147296462478866 0.0 58-59 4.064247626479386E-4 0.0 0.0 1.314458967355963 0.0 60-61 4.064247626479386E-4 0.0 0.0 1.459186825334894 0.0 62-63 4.064247626479386E-4 0.0 0.0 1.603914683313825 0.0 64-65 4.064247626479386E-4 0.0 0.0 1.787090323839251 0.0 66-67 4.064247626479386E-4 0.0 0.0 1.981645857718819 0.0 68-69 4.064247626479386E-4 0.0 0.0 2.225378787878788 0.0 70-71 4.064247626479386E-4 0.0 0.0 2.619041812979581 0.0 72-73 4.064247626479386E-4 0.0 0.0 2.926420860970217 0.0 74-75 4.064247626479386E-4 0.0 0.0 3.203236766809728 0.0 76-77 4.064247626479386E-4 0.0 0.0 3.541260241904019 0.0 78-79 4.064247626479386E-4 0.0 0.0 3.8433964104564966 0.0 80-81 4.877097151775263E-4 0.0 0.0 4.165284822473664 0.0 82-83 4.877097151775263E-4 0.0 0.0 4.589267134867994 0.0 84-85 5.283521914423202E-4 0.0 0.0 4.989880023410066 0.0 86-87 5.689946677071141E-4 0.0 0.0 5.390208414618286 0.0 88-89 5.689946677071141E-4 0.0 0.0 5.862067564052543 0.0 90-91 5.689946677071141E-4 0.0 0.0 6.282513981011835 0.0 92-93 5.689946677071141E-4 0.0 0.0 6.72214364676811 0.0 94-95 5.689946677071141E-4 0.0 0.0 7.207252243464691 0.0 96-97 5.689946677071141E-4 0.0 0.0 7.690978995968266 0.0 98-99 6.096371439719079E-4 0.0 0.0 8.251885810898687 0.0 100-101 7.315645727662896E-4 0.0 0.0 9.118830472103005 0.0 102-103 8.128495252958773E-4 0.0 0.0 9.79288594095461 0.0 104-105 9.754194303550526E-4 0.0 0.0 10.414471972948368 0.0 106-107 0.0012192742879438157 0.0 0.0 11.078895174925218 0.0 108-109 0.0013005592404734035 0.0 0.0 11.671665691247236 0.0 110-111 0.0013005592404734035 0.0 0.0 12.277888867212901 0.0 112-113 0.0013005592404734035 0.0 0.0 13.059687540642475 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 160 0.0 74.41461 2 ATAACGC 180 0.0 69.47067 3 AGTACTC 1455 0.0 51.567966 5 AAGTACT 1525 0.0 48.0275 4 AAAAAGT 2050 0.0 33.973846 1 AAAAGTA 2120 0.0 32.012325 2 AAAGTAC 2215 0.0 31.453344 3 GTATAAC 405 0.0 29.395964 1 GTACAAA 860 0.0 26.302553 1 TAACGCA 555 0.0 22.531029 4 GTAAGAC 160 2.5976918E-4 22.329859 3 TGCACGA 80 2.6023173E-4 22.325296 24-25 ATGGGGC 750 0.0 22.231524 5 GTCTTCG 135 0.0025486967 22.046972 1 CATGGGG 4915 0.0 21.928543 4 GTACAAG 830 0.0 21.51572 1 ATGGGGG 1445 0.0 21.429296 5 GTTTAGC 200 4.892439E-5 20.834389 1 ATGGGAG 2325 0.0 20.746008 5 GAACAAA 1170 0.0 20.351051 1 >>END_MODULE