##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1230240 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02204935622318 33.0 31.0 34.0 30.0 34.0 2 31.79393614254129 33.0 31.0 34.0 30.0 34.0 3 32.06426307062036 33.0 31.0 34.0 30.0 34.0 4 35.513366497593964 37.0 35.0 37.0 33.0 37.0 5 35.658524352971774 37.0 35.0 37.0 33.0 37.0 6 35.819896117830666 37.0 35.0 37.0 33.0 37.0 7 35.78560443490701 37.0 35.0 37.0 33.0 37.0 8 35.84865066978801 37.0 35.0 37.0 35.0 37.0 9 37.49556102874236 39.0 37.0 39.0 34.0 39.0 10-11 37.4451525718559 39.0 37.0 39.0 34.0 39.0 12-13 37.438818035505264 39.0 37.0 39.0 34.0 39.0 14-15 38.68378243269606 40.0 38.0 41.0 34.0 41.0 16-17 38.64562158603199 40.0 38.0 41.0 34.0 41.0 18-19 38.70324083105736 40.0 38.0 41.0 34.0 41.0 20-21 38.74963706268696 40.0 38.0 41.0 34.0 41.0 22-23 38.714075708804785 40.0 38.0 41.0 34.0 41.0 24-25 38.693323253999225 40.0 38.0 41.0 34.0 41.0 26-27 38.466024109116915 40.0 38.0 41.0 34.0 41.0 28-29 38.27510160619066 40.0 38.0 41.0 34.0 41.0 30-31 38.104594225516976 40.0 37.0 41.0 34.0 41.0 32-33 37.72433508908831 40.0 37.0 41.0 33.0 41.0 34-35 37.466957260371956 40.0 36.5 41.0 33.0 41.0 36-37 37.35134404669007 40.0 36.5 41.0 32.5 41.0 38-39 37.210304493432176 40.0 36.0 41.0 31.5 41.0 40-41 36.889184630641175 40.0 36.0 41.0 31.0 41.0 42-43 36.675807972428146 40.0 35.5 41.0 30.5 41.0 44-45 36.39122528937443 39.5 35.0 41.0 30.0 41.0 46-47 35.997046104825074 39.0 35.0 41.0 29.5 41.0 48-49 35.59586544088958 39.0 35.0 41.0 27.5 41.0 50-51 35.43853394459617 39.0 35.0 41.0 27.0 41.0 52-53 35.31763680257511 39.0 35.0 41.0 26.5 41.0 54-55 35.10004104890103 39.0 35.0 41.0 26.0 41.0 56-57 34.76447116009884 38.0 34.0 40.0 23.5 41.0 58-59 34.52762509754194 38.0 34.0 40.0 23.0 41.0 60-61 34.32434728183119 38.0 34.0 40.0 23.0 41.0 62-63 33.92497236311614 37.5 34.0 40.0 22.0 41.0 64-65 33.63945368383405 37.0 33.0 39.5 22.0 41.0 66-67 33.13919235271166 36.5 33.0 39.0 21.5 41.0 68-69 32.80212153726102 36.0 33.0 39.0 20.5 41.0 70-71 32.22401604564963 35.0 32.0 38.5 18.0 40.0 72-73 31.842117391728443 35.0 32.0 37.0 17.0 39.0 74-75 31.48955041292756 35.0 32.0 37.0 13.0 39.0 76-77 30.873014208609703 35.0 31.0 36.0 10.5 38.0 78-79 30.43378893549226 35.0 30.5 36.0 7.0 37.0 80-81 30.223837218754063 34.0 31.0 35.0 7.0 37.0 82-83 30.06693084276239 34.0 31.0 35.0 3.5 36.0 84-85 29.819444579919367 34.0 31.0 35.0 2.0 36.0 86-87 29.521915236051502 34.0 30.0 35.0 2.0 36.0 88-89 29.272190385615815 34.0 30.0 35.0 2.0 35.5 90-91 29.047348484848484 34.0 29.0 35.0 2.0 35.0 92-93 28.80087340681493 34.0 29.0 35.0 2.0 35.0 94-95 28.75292097476915 34.0 29.0 35.0 2.0 35.0 96-97 28.62614977565353 34.0 29.0 35.0 2.0 35.0 98-99 28.492690044219014 34.0 29.0 35.0 2.0 35.0 100-101 28.152973403563532 34.0 29.0 35.0 2.0 35.0 102-103 26.608816978800885 32.0 24.5 34.0 2.0 35.0 104-105 27.46224557809858 33.5 26.0 35.0 2.0 35.0 106-107 27.468373244245022 34.0 26.0 35.0 2.0 35.0 108-109 27.37765436012485 34.0 26.0 35.0 2.0 35.0 110-111 27.417065775783588 34.0 26.0 35.0 2.0 35.0 112-113 27.3950786025491 34.0 27.0 35.0 2.0 35.0 114-115 27.2683691799974 34.0 27.0 35.0 2.0 35.0 116-117 26.956512143971906 34.0 25.0 35.0 2.0 35.0 118-119 26.69672015216543 34.0 25.0 35.0 2.0 35.0 120-121 26.409634705423333 34.0 25.0 35.0 2.0 35.0 122-123 25.998773003641567 34.0 24.0 35.0 2.0 35.0 124-125 24.872775637274028 32.5 17.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3642234004567655 1101 2 0.6626930750468851 1101 3 0.5465775115788603 1101 4 0.580472734756647 1101 5 0.2567331297306126 1101 6 0.26682366956076464 1101 7 0.2241376741315264 1101 8 0.16167826470046975 1101 9 0.3687834845390938 1101 10-11 0.36215175616380435 1101 12-13 0.3276742045734764 1101 14-15 0.3739650301490869 1101 16-17 0.4359901632000245 1101 18-19 0.33052277617180437 1101 20-21 0.3312428627354933 1101 22-23 0.34549447173570513 1101 24-25 0.24365021109189655 1101 26-27 0.3541259920567015 1101 28-29 0.46078329279195174 1101 30-31 0.4733807924794746 1101 32-33 0.4712924688891036 1101 34-35 0.4624227953479192 1101 36-37 0.3880876967620992 1101 38-39 0.4356577357400653 1101 40-41 0.3928545487568016 1101 42-43 0.408090690306004 1101 44-45 0.4145893782202634 1101 46-47 0.44878716431351506 1101 48-49 0.5494453801333137 1101 50-51 0.5066171675522355 1101 52-53 0.48445228533527285 1101 54-55 0.4864355104505975 1101 56-57 0.5731469088134133 1101 58-59 0.6961237673569087 1101 60-61 0.6642651569320606 1101 62-63 0.7242696015273893 1101 64-65 0.5584073619774585 1101 66-67 0.5847588786581142 1101 68-69 0.7440531716435146 1101 70-71 0.6986349907204641 1101 72-73 0.6032849948010615 1101 74-75 0.6201699763347257 1101 76-77 0.5372768507256929 1101 78-79 0.6722185753500298 1101 80-81 0.5833948639376594 1101 82-83 0.46214499300693745 1101 84-85 0.450148261219816 1101 86-87 0.5628703865298519 1101 88-89 0.6237015732517293 1101 90-91 0.6456368614527825 1101 92-93 0.5716325425275031 1101 94-95 0.49195951674680316 1101 96-97 0.5336150137307705 1101 98-99 0.6139294886386466 1101 100-101 0.5381104449170273 1101 102-103 0.5098484790084719 1101 104-105 0.5718264219998872 1101 106-107 0.6655827236985843 1101 108-109 0.587503322487521 1101 110-111 0.5912637571604655 1101 112-113 0.5858267966306698 1101 114-115 0.673653420830636 1101 116-117 0.5945012226885353 1101 118-119 0.5879209053258982 1101 120-121 0.6485464587642227 1101 122-123 0.8919741856955667 1101 124-125 0.9439135044890534 1102 1 0.33818761751003734 1102 2 0.5940507578627781 1102 3 0.411014026407841 1102 4 0.5063014871425935 1102 5 0.28847084595552985 1102 6 0.2119451038116651 1102 7 0.20489912726380055 1102 8 0.20569803743659776 1102 9 0.24045919471006272 1102 10-11 0.21472969910265505 1102 12-13 0.2058472601245782 1102 14-15 0.3849408292274745 1102 16-17 0.41167810730750176 1102 18-19 0.29010870932167165 1102 20-21 0.23845862822153663 1102 22-23 0.21187970325017602 1102 24-25 0.24090737123295725 1102 26-27 0.31081470035556436 1102 28-29 0.3564191813735391 1102 30-31 0.30433123117345673 1102 32-33 0.26343026279344883 1102 34-35 0.2923947940922176 1102 36-37 0.40437760485289687 1102 38-39 0.45871194062585374 1102 40-41 0.5249571176772321 1102 42-43 0.577822238005524 1102 44-45 0.643634981083629 1102 46-47 0.6122785794768149 1102 48-49 0.530124055412692 1102 50-51 0.5279141332426747 1102 52-53 0.5648746929849793 1102 54-55 0.4356786516323865 1102 56-57 0.5735355923648484 1102 58-59 0.5648015730391904 1102 60-61 0.7241550767091454 1102 62-63 0.674608761581375 1102 64-65 0.5724986444670535 1102 66-67 0.5461653517143574 1102 68-69 0.6780570246804629 1102 70-71 0.6113488955666142 1102 72-73 0.5054806701881382 1102 74-75 0.5190741579625104 1102 76-77 0.45313230398170035 1102 78-79 0.47077122321018194 1102 80-81 0.4338837401982296 1102 82-83 0.38586162121579903 1102 84-85 0.3528035112261172 1102 86-87 0.42063583705905927 1102 88-89 0.44003857019725245 1102 90-91 0.3548368321225013 1102 92-93 0.2631991119612209 1102 94-95 0.38998894556243613 1102 96-97 0.41160661679120025 1102 98-99 0.4224560084858524 1102 100-101 0.5250694959448445 1102 102-103 0.3280819194278948 1102 104-105 0.49447782240638816 1102 106-107 0.4690010766656414 1102 108-109 0.6207834564902583 1102 110-111 0.645775980785551 1102 112-113 0.6087924950924304 1102 114-115 0.6288665924196692 1102 116-117 0.5940150102135426 1102 118-119 0.6266268554781611 1102 120-121 0.6729576189373603 1102 122-123 0.610039836266477 1102 124-125 0.7099827193769244 1103 1 0.29201716236246966 1103 2 0.5572631953837508 1103 3 0.41340500823373105 1103 4 0.5378412529455971 1103 5 0.2210508985880395 1103 6 0.28864859557238276 1103 7 0.2811853562681961 1103 8 0.2087888726685847 1103 9 0.26767179118827755 1103 10-11 0.32416734370958267 1103 12-13 0.2738942054962692 1103 14-15 0.45877977904996925 1103 16-17 0.38152369801026964 1103 18-19 0.3120933787789255 1103 20-21 0.29660275828388194 1103 22-23 0.3480729422688782 1103 24-25 0.31943887125945025 1103 26-27 0.185486851047024 1103 28-29 0.32857958689111655 1103 30-31 0.36942532502887104 1103 32-33 0.3976365363058676 1103 34-35 0.38390117887470865 1103 36-37 0.3406556210665457 1103 38-39 0.4080465279551859 1103 40-41 0.48639122107009314 1103 42-43 0.42944117334524634 1103 44-45 0.457152748970735 1103 46-47 0.41042937691230463 1103 48-49 0.5469911848215574 1103 50-51 0.5109023968997519 1103 52-53 0.47596902527625673 1103 54-55 0.4694239491473553 1103 56-57 0.37767709080177525 1103 58-59 0.5098928851429037 1103 60-61 0.42860720408688024 1103 62-63 0.44965335791829375 1103 64-65 0.36294001573818235 1103 66-67 0.40506761950432235 1103 68-69 0.395870391218196 1103 70-71 0.5088082290059148 1103 72-73 0.48490453850089565 1103 74-75 0.4424965073505227 1103 76-77 0.5056996841897572 1103 78-79 0.46853478314589125 1103 80-81 0.41583657405258734 1103 82-83 0.43386118849886657 1103 84-85 0.4829839718488742 1103 86-87 0.5217403691066025 1103 88-89 0.6073090536038848 1103 90-91 0.5998914957513186 1103 92-93 0.4603679068887203 1103 94-95 0.30538505817480655 1103 96-97 0.4268808621858575 1103 98-99 0.534103600283661 1103 100-101 0.5278733250481515 1103 102-103 0.2750641603717483 1103 104-105 0.3510972876035865 1103 106-107 0.512542114126564 1103 108-109 0.5590153221184018 1103 110-111 0.5753535077641239 1103 112-113 0.6182129193316435 1103 114-115 0.5966225577316671 1103 116-117 0.6221559611310781 1103 118-119 0.6674247766706962 1103 120-121 0.7524461740160433 1103 122-123 0.6849451242440097 1103 124-125 0.568732653144739 1104 1 0.2939924375110152 1104 2 0.5750655126826594 1104 3 0.08900772254145295 1104 4 0.39299723185813207 1104 5 0.19878251125212643 1104 6 0.2013621999345645 1104 7 0.18749334177223886 1104 8 0.14495346162602374 1104 9 0.27817672700369656 1104 10-11 0.2571694549122441 1104 12-13 0.24224207497906747 1104 14-15 0.273872589349871 1104 16-17 0.3845926732675551 1104 18-19 0.2876907620487259 1104 20-21 0.32762591006049036 1104 22-23 0.22502435334716608 1104 24-25 0.27129959887076893 1104 26-27 0.1480629648711087 1104 28-29 0.3219069974692417 1104 30-31 0.16481073953242031 1104 32-33 0.3292140602034337 1104 34-35 0.3747357012248784 1104 36-37 0.3629735507705263 1104 38-39 0.44718430095456796 1104 40-41 0.5092213211707985 1104 42-43 0.5739970113620885 1104 44-45 0.5834101482280758 1104 46-47 0.42816498645927226 1104 48-49 0.498595408566608 1104 50-51 0.5507436715257938 1104 52-53 0.7978134136750228 1104 54-55 0.6784037057170877 1104 56-57 0.6198542811047503 1104 58-59 0.7608107447339734 1104 60-61 0.7295297806696084 1104 62-63 0.546628426524002 1104 64-65 0.622281752573592 1104 66-67 0.5718481638395758 1104 68-69 0.7022430469570722 1104 70-71 0.7653716856620605 1104 72-73 0.777896568180541 1104 74-75 0.6781504331156789 1104 76-77 0.5456953077146487 1104 78-79 0.5266264892789891 1104 80-81 0.5984269951543446 1104 82-83 0.4883844485708977 1104 84-85 0.27832584489090806 1104 86-87 0.4257294926728079 1104 88-89 0.42972827302828875 1104 90-91 0.3777742196158336 1104 92-93 0.3430741873537855 1104 94-95 0.3011960067311321 1104 96-97 0.26535013419495 1104 98-99 0.31142168070809007 1104 100-101 0.3663645073439703 1104 102-103 0.3464141019815301 1104 104-105 0.5462742613157054 1104 106-107 0.37171730487166244 1104 108-109 0.450921969224904 1104 110-111 0.40119463948486 1104 112-113 0.5539187878274987 1104 114-115 0.46026465584956 1104 116-117 0.49157790915373667 1104 118-119 0.46592396389674917 1104 120-121 0.5185996375718425 1104 122-123 0.3002295169745963 1104 124-125 0.4475362510091969 1105 1 0.15557795106848005 1105 2 0.4509523027951623 1105 3 0.07962872383383512 1105 4 0.34958863162446363 1105 5 0.1456556174934036 1105 6 0.11863211146483366 1105 7 0.15176059150685006 1105 8 0.08507237611622287 1105 9 0.1516484054812537 1105 10-11 0.07771002942736516 1105 12-13 0.12192006751067908 1105 14-15 0.21585275840212859 1105 16-17 0.19667006697163458 1105 18-19 0.06888384499546873 1105 20-21 0.058168395194783784 1105 22-23 0.16080390961073476 1105 24-25 0.27430611249693015 1105 26-27 0.21164152955947202 1105 28-29 0.3105872045948175 1105 30-31 0.2353520788514274 1105 32-33 0.16432748618279192 1105 34-35 0.11238641278451666 1105 36-37 0.2270635884906511 1105 38-39 0.290125703349041 1105 40-41 0.3185817142855569 1105 42-43 0.1708841760894373 1105 44-45 0.31558611541993997 1105 46-47 0.2050748895016028 1105 48-49 0.27828335745434885 1105 50-51 0.39126843267753486 1105 52-53 0.44197978160465823 1105 54-55 0.5193041941625651 1105 56-57 0.4708340505122095 1105 58-59 0.47246048791282647 1105 60-61 0.41088995240841086 1105 62-63 0.41156892617177476 1105 64-65 0.3280263753764814 1105 66-67 0.5606510176983406 1105 68-69 0.5972801093536688 1105 70-71 0.34005534191414455 1105 72-73 0.3964660622947527 1105 74-75 0.3711537011922381 1105 76-77 0.43254104231991164 1105 78-79 0.42549800027839524 1105 80-81 0.3509340918391821 1105 82-83 0.4189188406894715 1105 84-85 0.5189533122525525 1105 86-87 0.4225586157375396 1105 88-89 0.44721836464623266 1105 90-91 0.4316154402094732 1105 92-93 0.360359311528736 1105 94-95 0.284015173995968 1105 96-97 0.510637899307266 1105 98-99 0.5541431811253972 1105 100-101 0.4072402161433608 1105 102-103 0.4114603948658271 1105 104-105 0.5187976385895716 1105 106-107 0.4464870363253759 1105 108-109 0.5741104616796271 1105 110-111 0.4889435101476245 1105 112-113 0.43564575951278783 1105 114-115 0.35766996826082575 1105 116-117 0.44569954857067273 1105 118-119 0.4294816146750513 1105 120-121 0.47272850786193743 1105 122-123 0.39388197840553474 1105 124-125 0.36459034878673435 1106 1 0.1625799586070471 1106 2 0.40729017049971006 1106 3 0.36371197694056434 1106 4 0.4789893585014511 1106 5 0.1766816498145971 1106 6 0.1420117130845071 1106 7 0.06523408793069052 1106 8 0.013863490301389447 1106 9 0.14884209543918558 1106 10-11 0.18798141721575945 1106 12-13 0.16683012235612438 1106 14-15 0.24239574263965125 1106 16-17 0.3323297317149638 1106 18-19 0.11839113031173554 1106 20-21 0.1390119251584423 1106 22-23 0.17587270070976047 1106 24-25 0.20329502493242302 1106 26-27 0.15896858808223868 1106 28-29 0.36242689252323856 1106 30-31 0.23272626287049292 1106 32-33 0.35089948588268527 1106 34-35 0.2823626845729521 1106 36-37 0.19054404448256435 1106 38-39 0.24794655269928256 1106 40-41 0.3375008769446879 1106 42-43 0.3306733327539888 1106 44-45 0.42182903743816524 1106 46-47 0.5370250665727241 1106 48-49 0.6615330033500086 1106 50-51 0.7801061949149499 1106 52-53 0.7531917089149402 1106 54-55 0.7356980061845064 1106 56-57 0.8076344528788155 1106 58-59 0.7334652575536467 1106 60-61 0.7538807074242868 1106 62-63 0.6874741184137392 1106 64-65 0.7055504818803513 1106 66-67 0.8136554417606163 1106 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-1.9875908147546184 2216 94-95 -2.1235282756170477 2216 96-97 -2.326647743795551 2216 98-99 -2.4400297949500143 2216 100-101 -2.5750774011345463 2216 102-103 -2.544494054605842 2216 104-105 -2.685577947675025 2216 106-107 -2.7539405709726488 2216 108-109 -2.664792236600949 2216 110-111 -2.6847101693453546 2216 112-113 -2.8345056805743596 2216 114-115 -2.9338765424207907 2216 116-117 -2.9757984618775097 2216 118-119 -2.7557475871198953 2216 120-121 -2.6943388770517345 2216 122-123 -2.8886509416454658 2216 124-125 -3.001624391457895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28.0 3 22.0 4 58.0 5 173.0 6 591.0 7 1027.0 8 1794.0 9 3713.0 10 7899.0 11 10278.0 12 8971.0 13 10665.0 14 14226.0 15 14055.0 16 9119.0 17 8692.0 18 8860.0 19 8754.0 20 9078.0 21 8840.0 22 9231.0 23 9925.0 24 10985.0 25 12669.0 26 14415.0 27 17416.0 28 21110.0 29 25852.0 30 32060.0 31 39841.0 32 51931.0 33 67276.0 34 93178.0 35 137301.0 36 196294.0 37 262588.0 38 100747.0 39 578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.60834099919407 17.442506044497268 10.708406939164272 23.240746017144392 2 11.980953247994695 23.75121521399146 35.45189703439015 28.815934503623698 3 23.395085662356117 29.911472342321034 28.33714154264585 18.356300452677004 4 14.003121342177135 19.433606450773834 32.49748016647158 34.06579204057745 5 10.832357913902978 39.461080764728834 34.94960332943165 14.756957991936535 6 31.85551163276537 27.71419676163581 20.38109591290444 20.049195692694376 7 21.799161139289893 32.652815710755625 24.64072050981922 20.90730264013526 8 28.49769150734816 31.584650149564315 23.765119001170504 16.152539341917024 9 29.74159513590844 14.032627779945376 21.836308362595915 34.389468721550266 10-11 23.454904512495332 27.551017505128065 26.17384663717918 22.82023134519742 12-13 27.978139944350016 25.504089209056495 25.863221017258326 20.654549829335167 14-15 28.93334975315401 22.127943730412554 26.19054876747705 22.748157748956384 16-17 22.32597836998622 26.24758684316411 33.355497120468854 18.07093766638081 18-19 25.115018207829365 24.142443750812852 33.06480849265184 17.677729548705944 20-21 20.607314615778847 24.742620416985204 34.5013210885233 20.14874387871265 22-23 19.429298348289763 26.997943490701005 27.84099037586162 25.731767785147614 24-25 20.842396605540383 28.847338730654183 26.376560671088566 23.933703992716868 26-27 23.43367960723111 23.009534724931722 28.04692580309533 25.50985986474184 28-29 22.353972064698183 22.481630549598027 32.047481965851254 23.11691541985254 30-31 25.555663935492262 24.724891078163612 30.242594940824553 19.476850045519573 32-33 18.636526206268694 24.985653205878528 32.70374073351541 23.674079854337364 34-35 16.42827125460988 26.986463605852194 34.103292124538406 22.481973014999525 36-37 23.889159838730652 24.654335739367927 29.432021394199502 22.024483027701912 38-39 25.903360319937573 22.93678469241774 29.247341982052284 21.912513005592405 40-41 21.535806645779136 26.07768649925482 29.545141413521513 22.84136544144453 42-43 25.320953635063077 24.902214202106908 28.391330146963195 21.385502015866823 44-45 27.510770256210172 21.7498618155807 28.456032969176746 22.283334959032384 46-47 22.478825269866043 24.468030628170112 34.804875471452725 18.24826863051112 48-49 25.40390492911952 24.176989855637924 31.946164975939656 18.4729402393029 50-51 20.50579561711536 25.404799063597345 32.71085316686175 21.37855215242554 52-53 19.540171023540122 27.639810118350887 26.23610027311744 26.583918584991544 54-55 21.02736051502146 28.962153726102223 24.831577578358694 25.178908180517624 56-57 23.64664618285863 23.63937117960723 26.17838795682143 26.535594680712705 58-59 22.86842404734036 22.935362205748472 29.969599427753934 24.22661431915724 60-61 25.664992196644558 25.179599102614127 28.38275458447132 20.772654116269994 62-63 19.102126414358175 25.508315450643774 30.748024775653533 24.641533359344518 64-65 16.83777149174145 27.10597119261282 32.442572181037846 23.61368513460788 66-67 23.82612335804396 25.163098257250617 27.922803680582653 23.087974704122775 68-69 25.725224346468984 23.51947587462609 27.7509672909351 23.00433248796983 70-71 21.70222883339836 26.22671186110027 28.180720834959033 23.890338470542332 72-73 25.142614449213163 25.194799388737156 27.1593347639485 22.503251398101185 74-75 27.07235986474184 22.126251788268956 27.44013363246196 23.361254714527245 76-77 22.192011314865393 24.654701521654314 33.70159481076863 19.451692352711667 78-79 24.764517492521783 24.40857068539472 31.183305696449477 19.643606125634022 80-81 20.117294186500196 25.513029977890493 31.986279100013004 22.383396735596307 82-83 19.215396995708154 27.3741708934842 25.970786188060867 27.43964592274678 84-85 20.642598192222657 28.47200546234881 24.76049388737157 26.124902458056965 86-87 22.938776173754714 23.431119131226428 26.107060410976718 27.523044284042136 88-89 22.216559370529325 22.771166601638704 29.661976524905707 25.350297502926256 90-91 24.72302152425543 24.987766614644297 28.021686825334896 22.267525035765377 92-93 18.777636883860062 25.257429444661206 30.02629568214332 25.938637989335415 94-95 16.767711663086708 26.699695787974175 31.65447041299272 24.878122135946402 96-97 23.205872502861304 24.736386042035168 27.621711125273123 24.436030329830402 98-99 24.741148024514885 23.423182488254877 27.41471551380415 24.420953973426087 100-101 21.131892963974508 25.609555859019377 27.927843347639485 25.330707829366627 102-103 24.107254628131823 24.641438667397175 27.236842319178926 24.014464385292076 104-105 25.81660673887813 21.70758626857541 27.625538384612412 24.850268607934044 106-107 21.37799161315814 24.121113280900666 33.126724562650104 21.374170543291097 108-109 23.814719346077844 24.234680914980444 30.387965464858148 21.56263427408356 110-111 19.457719882100292 25.129214485680617 31.465734330545995 23.947331301673092 112-113 18.666452042466325 26.63104230129173 26.031232698972484 28.671272957269462 114-115 19.7008280289739 27.9588364088792 24.983258486128022 27.357077076018875 116-117 21.83462752933389 23.25096505630139 26.12832504283492 28.786082371529805 118-119 21.019382487344 22.842463985339247 29.199020610992065 26.93913291632468 120-121 23.352687339845733 24.654164336164623 27.855420530964242 24.137727793025405 122-123 18.16979501723702 25.159547098400992 29.24408287751351 27.426575006848473 124-125 16.33798695360605 26.529252778963198 30.635696722144324 26.49706354528643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1128.0 1 938.5 2 2323.5 3 3059.5 4 1505.5 5 882.5 6 1290.5 7 2506.0 8 3751.0 9 3889.5 10 2980.0 11 1971.0 12 1685.5 13 1748.5 14 1944.0 15 2357.0 16 2549.5 17 2654.0 18 2893.5 19 2946.5 20 3048.0 21 3329.5 22 3701.0 23 4090.5 24 4452.5 25 5020.0 26 5875.0 27 6612.5 28 7102.5 29 7853.0 30 8988.5 31 10003.5 32 10906.0 33 11991.5 34 13286.0 35 14047.5 36 14847.0 37 15916.5 38 16140.0 39 15958.5 40 15781.5 41 16686.0 42 17809.5 43 18627.0 44 20239.0 45 21777.5 46 25468.5 47 36113.5 48 48708.0 49 87689.5 50 223033.5 51 225397.0 52 81927.0 53 30366.0 54 20852.0 55 17136.5 56 14592.0 57 12590.5 58 11481.0 59 10432.5 60 9257.5 61 8097.5 62 6906.5 63 5309.0 64 3854.0 65 3023.5 66 2133.0 67 1619.5 68 1259.5 69 825.0 70 678.5 71 627.5 72 612.0 73 569.5 74 401.0 75 320.5 76 214.5 77 101.5 78 54.0 79 26.0 80 19.0 81 5.0 82 3.5 83 2.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1503771621797373 2 3.251398101183509E-4 3 2.438548575887632E-4 4 0.0 5 0.0 6 0.004145532579008974 7 0.0 8 0.0 9 0.0 10-11 4.064247626479386E-5 12-13 0.004145532579008974 14-15 0.0015850565743269606 16-17 4.064247626479386E-4 18-19 0.0 20-21 3.657822863831447E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.657822863831447E-4 30-31 0.0 32-33 0.0 34-35 8.128495252958772E-5 36-37 0.0 38-39 0.0 40-41 4.064247626479386E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0010160619066198464 96-97 0.002601118480946807 98-99 0.003942320197685005 100-101 0.0 102-103 0.003739107816361035 104-105 0.02336942385225647 106-107 0.017720119651450126 108-109 0.02003674079854337 110-111 0.017191767460007803 112-113 0.014875146312914552 114-115 0.04165853817141371 116-117 0.04218689036285603 118-119 0.023531993757315645 120-121 0.014306151645207441 122-123 0.003170113148653921 124-125 0.001219274287943816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1230240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.021000018485964 #Duplication Level Percentage of deduplicated Percentage of total 1 87.25050805938189 43.643576652512515 2 8.866504663336357 8.870228598573075 3 1.9626978121125496 2.945283218878926 4 0.7057955326221839 1.4121839340136628 5 0.3383784394003177 0.8463013961749271 6 0.18061386095428464 0.5420691565279856 7 0.12036667715356042 0.42146030920863387 8 0.09055750131534272 0.36238214199750407 9 0.06553024311724116 0.29500994629610194 >10 0.3564515457879587 3.235107855933936 >50 0.029802633440671127 1.0274843551548234 >100 0.021914574019690526 2.431725508344894 >500 0.004729764068679793 1.5578500131032913 >1k 0.0025225408366292227 2.8684660588542075 >5k 0.00220722323205057 7.706523293027575 >10k+ 0.0014189292206039379 21.834347561397987 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 68517 5.569401092469762 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 60283 4.900100793341137 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 36953 3.0037228508258553 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 21568 1.753153856158148 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 21450 1.7435622317596564 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 19666 1.5985498764468722 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18742 1.523442580309533 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 17176 1.3961503446481986 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12511 1.0169560410976721 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9527 0.7744017427493822 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9285 0.7547307842372221 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8512 0.6918975159318507 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8105 0.6588145402523086 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7976 0.6483287813759917 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7036 0.5719209259981792 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6409 0.5209552607621277 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6357 0.5167284432305892 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6318 0.5135583300819352 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6265 0.5092502275978671 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5711 0.46421836389647547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5624 0.4571465730264014 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5341 0.434142931460528 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 5255 0.4271524255429835 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4777 0.38829821823384053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4415 0.3588730654181298 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3919 0.3185557289634543 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3413 0.2774255429834829 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2464 0.20028612303290413 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2328 0.18923136948888022 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2185 0.17760762127714919 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1887 0.15338470542333205 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 1847 0.15013330732214852 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1701 0.13826570425282872 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 1423 0.11566848744960334 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1373 0.11160423982312395 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.6256990505917544E-4 0.0 6 0.0 0.0 0.0 1.6256990505917544E-4 0.0 7 0.0 0.0 0.0 1.6256990505917544E-4 0.0 8 0.0 0.0 0.0 1.6256990505917544E-4 0.0 9 0.0 0.0 0.0 1.6256990505917544E-4 0.0 10-11 0.0 0.0 0.0 4.877097151775264E-4 0.0 12-13 0.0 0.0 0.0 0.0012599167642086098 0.0 14-15 0.0 0.0 0.0 0.0030888281961243334 0.0 16-17 0.0 0.0 0.0 0.005324164390687995 0.0 18-19 0.0 0.0 0.0 0.007356288203927689 0.0 20-21 0.0 0.0 0.0 0.010241904018728053 0.0 22-23 0.0 0.0 0.0 0.014590648979060997 0.0 24-25 0.0 0.0 0.0 0.020036740798543375 0.0 26-27 0.0 0.0 0.0 0.02536090518923137 0.0 28-29 0.0 0.0 0.0 0.0324326960593055 0.0 30-31 0.0 0.0 0.0 0.04324359474574067 0.0 32-33 0.0 0.0 0.0 0.0649060345948758 1.6256990505917544E-4 34-35 0.0 0.0 0.0 0.09595688646117831 1.6256990505917544E-4 36-37 8.128495252958772E-5 0.0 0.0 0.1355426583430875 1.6256990505917544E-4 38-39 8.128495252958772E-5 0.0 0.0 0.20105833008193524 1.6256990505917544E-4 40-41 8.128495252958772E-5 0.0 0.0 0.31258128495252957 1.6256990505917544E-4 42-43 1.2192742879438158E-4 0.0 0.0 0.38585966965795293 1.6256990505917544E-4 44-45 1.6256990505917544E-4 0.0 0.0 0.4629178046560021 1.6256990505917544E-4 46-47 1.6256990505917544E-4 0.0 0.0 0.5653774873195474 1.6256990505917544E-4 48-49 1.6256990505917544E-4 0.0 0.0 0.6574326960593055 1.6256990505917544E-4 50-51 1.6256990505917544E-4 0.0 0.0 0.7565596956691377 1.6256990505917544E-4 52-53 1.6256990505917544E-4 0.0 0.0 0.8879568214332163 1.6256990505917544E-4 54-55 1.6256990505917544E-4 0.0 0.0 1.0193945896735597 1.6256990505917544E-4 56-57 1.6256990505917544E-4 0.0 0.0 1.1540024710625567 1.6256990505917544E-4 58-59 1.6256990505917544E-4 0.0 0.0 1.3219778254649501 1.6256990505917544E-4 60-61 1.6256990505917544E-4 0.0 0.0 1.468859734685915 1.6256990505917544E-4 62-63 1.6256990505917544E-4 0.0 0.0 1.615335219144232 1.6256990505917544E-4 64-65 1.6256990505917544E-4 0.0 0.0 1.800299128625309 1.6256990505917544E-4 66-67 2.4385485758876316E-4 0.0 0.0 1.9959520093640264 1.6256990505917544E-4 68-69 2.4385485758876316E-4 0.0 0.0 2.2409042138119393 1.6256990505917544E-4 70-71 2.4385485758876316E-4 0.0 0.0 2.6355020158668228 1.6256990505917544E-4 72-73 2.4385485758876316E-4 0.0 0.0 2.943693913382755 1.6256990505917544E-4 74-75 2.8449733385355703E-4 0.0 0.0 3.2227451554168294 1.6256990505917544E-4 76-77 3.251398101183509E-4 0.0 0.0 3.563247821563272 1.6256990505917544E-4 78-79 3.251398101183509E-4 0.0 0.0 3.866928404213812 1.6256990505917544E-4 80-81 4.064247626479386E-4 0.0 0.0 4.191092794901808 1.6256990505917544E-4 82-83 4.064247626479386E-4 0.0 0.0 4.617798153205879 1.6256990505917544E-4 84-85 4.470672389127325E-4 0.0 0.0 5.022353361945637 1.6256990505917544E-4 86-87 4.877097151775263E-4 0.0 0.0 5.426502145922747 1.6256990505917544E-4 88-89 4.877097151775263E-4 0.0 0.0 5.900149564312654 1.6256990505917544E-4 90-91 5.689946677071141E-4 0.0 0.0 6.324863441279751 1.6256990505917544E-4 92-93 6.502796202367018E-4 0.0 0.0 6.7705894784757445 1.6256990505917544E-4 94-95 6.502796202367018E-4 0.0 0.0 7.263135648328781 1.6256990505917544E-4 96-97 6.502796202367018E-4 0.0 0.0 7.7536090518923135 1.6256990505917544E-4 98-99 6.502796202367018E-4 0.0 0.0 8.323213356743398 1.6256990505917544E-4 100-101 6.909220965014957E-4 0.0 0.0 9.201172129015475 1.6256990505917544E-4 102-103 7.315645727662895E-4 0.0 0.0 9.88563207179087 1.6256990505917544E-4 104-105 7.315645727662895E-4 0.0 0.0 10.516281375991676 1.6256990505917544E-4 106-107 7.315645727662895E-4 0.0 0.0 11.193506957991936 1.6256990505917544E-4 108-109 7.315645727662895E-4 0.0 0.0 11.799486279100012 1.6256990505917544E-4 110-111 7.315645727662895E-4 0.0 0.0 12.420869098712446 1.6256990505917544E-4 112-113 7.315645727662895E-4 0.0 0.0 13.222541943035505 1.6256990505917544E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1665 0.0 56.45658 5 AAGTACT 1730 0.0 53.647594 4 TATAACG 125 0.0 52.35455 2 AAAGTAC 1755 0.0 51.86639 3 GTTAACG 60 2.19483E-5 49.578167 2 AAAAGTA 1895 0.0 47.720623 2 TATCACG 50 5.3354906E-4 47.59504 2 AAAAAGT 1970 0.0 45.369923 1 ATAACGC 135 1.3278623E-9 39.662537 3 GTCTAGC 95 7.399134E-6 37.633156 1 GTTATCA 535 0.0 32.298862 1 GTATAAC 335 0.0 28.458904 1 ATCACGC 110 7.756267E-4 27.042639 3 GTCGGGA 330 0.0 27.042637 2 TTATCAA 695 0.0 26.536804 2 TGGACCG 90 0.009534059 26.44169 5 GTACTTG 600 0.0 25.820526 1 GTGTTAT 380 0.0 25.088772 1 TAACGCA 290 1.4551915E-10 24.618124 4 CGGGAGT 420 0.0 24.080826 4 >>END_MODULE