##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364144_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1418501 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18525119122228 31.0 31.0 34.0 26.0 34.0 2 30.277821446724396 31.0 31.0 34.0 26.0 34.0 3 30.439473077565683 31.0 31.0 34.0 26.0 34.0 4 33.755799255693155 37.0 35.0 37.0 28.0 37.0 5 33.792799582094055 37.0 35.0 37.0 30.0 37.0 6 33.691971313379405 37.0 35.0 37.0 28.0 37.0 7 33.587259367459026 37.0 35.0 37.0 28.0 37.0 8 33.48028658421813 37.0 35.0 37.0 27.0 37.0 9 34.81784996979206 39.0 35.0 39.0 26.0 39.0 10-11 34.76100968557654 39.0 35.0 39.0 26.5 39.0 12-13 34.62327273650142 39.0 35.0 39.0 25.5 39.0 14-15 35.76125818734002 40.0 36.0 41.0 25.0 41.0 16-17 35.790283193314636 40.0 36.0 41.0 25.0 41.0 18-19 35.722459483638005 40.0 36.0 41.0 25.0 41.0 20-21 35.75167659381276 40.0 36.0 41.0 25.5 41.0 22-23 35.76920037419784 40.0 36.0 41.0 25.5 41.0 24-25 35.78737025916796 40.0 36.0 41.0 26.0 41.0 26-27 35.60898476631317 40.0 36.0 41.0 25.0 41.0 28-29 35.488337336385385 40.0 35.5 41.0 25.0 41.0 30-31 35.5468441685977 40.0 35.0 41.0 25.0 41.0 32-33 35.20531709177505 39.0 35.0 41.0 24.5 41.0 34-35 35.264627589265004 39.0 35.0 41.0 25.0 41.0 36-37 35.276602554386635 40.0 35.0 41.0 25.0 41.0 38-39 35.10314197875081 39.5 35.0 41.0 24.5 41.0 40-41 34.73257544407794 39.0 34.0 41.0 24.0 41.0 42-43 34.472855852762876 39.0 33.0 41.0 23.0 41.0 44-45 34.177505690866624 38.0 33.0 40.5 22.0 41.0 46-47 33.89757356533411 38.0 33.0 40.0 20.0 41.0 48-49 33.592300252167604 38.0 33.0 40.0 16.5 41.0 50-51 33.474440624292825 38.0 33.0 40.0 15.5 41.0 52-53 33.30886901031441 38.0 32.5 40.0 13.5 41.0 54-55 32.99156433446293 38.0 32.0 40.0 10.0 41.0 56-57 32.55936619008376 37.0 31.0 40.0 8.0 41.0 58-59 32.10538942165004 37.0 31.0 40.0 4.0 41.0 60-61 31.822251447126227 36.0 30.0 40.0 2.0 41.0 62-63 31.23034703535634 36.0 29.5 39.0 2.0 41.0 64-65 30.99986323590889 35.5 29.0 39.0 2.0 41.0 66-67 30.555461011307006 35.0 29.5 38.5 2.0 40.5 68-69 30.113603021781444 35.0 29.0 38.0 2.0 40.0 70-71 29.424484015168126 34.0 28.0 37.0 2.0 40.0 72-73 29.1959328897195 34.0 28.0 37.0 2.0 39.0 74-75 28.82616579050702 34.0 28.0 36.0 2.0 39.0 76-77 28.208649482799096 34.0 26.0 36.0 2.0 38.5 78-79 27.84870331427331 34.0 26.0 35.0 2.0 37.0 80-81 27.621132448972542 34.0 26.0 35.0 2.0 37.0 82-83 27.423350424144925 34.0 26.0 35.0 2.0 36.5 84-85 27.132567759909932 34.0 26.0 35.0 2.0 36.0 86-87 26.723229662862416 33.0 25.0 35.0 2.0 36.0 88-89 26.32326448835778 33.0 24.0 35.0 2.0 35.5 90-91 26.18520395826298 33.0 23.5 35.0 2.0 35.0 92-93 25.80769629348164 33.0 21.5 35.0 2.0 35.0 94-95 25.72168859944406 33.0 20.0 35.0 2.0 35.0 96-97 25.599802538031344 33.0 20.0 35.0 2.0 35.0 98-99 25.35149605111311 33.0 19.0 35.0 2.0 35.0 100-101 24.747638528277385 32.0 16.0 35.0 2.0 35.0 102-103 23.50321325117148 30.0 10.0 34.0 2.0 35.0 104-105 24.222309677610376 31.5 10.0 35.0 2.0 35.0 106-107 24.164239926513975 32.0 7.0 35.0 2.0 35.0 108-109 24.11635381293351 32.0 7.0 35.0 2.0 35.0 110-111 24.163313949020832 32.0 3.5 35.0 2.0 35.0 112-113 24.06513037354221 32.0 2.0 35.0 2.0 35.0 114-115 23.878055426115317 32.0 2.0 35.0 2.0 35.0 116-117 23.46866692374556 32.0 2.0 35.0 2.0 35.0 118-119 23.174844078361595 32.0 2.0 35.0 2.0 35.0 120-121 22.985276711119695 32.0 2.0 35.0 2.0 35.0 122-123 22.477048306627914 31.5 2.0 35.0 2.0 35.0 124-125 21.46032995394434 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3457736928369535 1101 2 0.47665175047984576 1101 3 0.4348240496906719 1101 4 0.49215244007424985 1101 5 0.40408408180743294 1101 6 0.4451265207206916 1101 7 0.5382966268959422 1101 8 0.5961514837668815 1101 9 0.6988718872328334 1101 10-11 0.6557065852788142 1101 12-13 0.6283499820296043 1101 14-15 0.5659977855750569 1101 16-17 0.6054036956923028 1101 18-19 0.7558136162020475 1101 20-21 0.8083765642527467 1101 22-23 0.636994506329394 1101 24-25 0.7366887031925344 1101 26-27 0.7119376232679357 1101 28-29 0.7613401315458503 1101 30-31 0.6311437512176354 1101 32-33 0.765659602151544 1101 34-35 0.8343424242456905 1101 36-37 0.8816713508236447 1101 38-39 0.8290368923597455 1101 40-41 0.7705745919924851 1101 42-43 0.9371279326631097 1101 44-45 0.9314809452512165 1101 46-47 1.047750925570874 1101 48-49 1.0073927084244332 1101 50-51 0.9811318676113814 1101 52-53 0.9381608353055881 1101 54-55 0.8223797923215344 1101 56-57 0.7499481210350822 1101 58-59 0.8367974485435354 1101 60-61 0.7126209111208155 1101 62-63 0.8354482702027894 1101 64-65 0.878215778432228 1101 66-67 1.0674407180855852 1101 68-69 1.0738974105119752 1101 70-71 0.9463278341726209 1101 72-73 0.9685389596347989 1101 74-75 0.9532487212992073 1101 76-77 1.0046624214214361 1101 78-79 0.8931717555759953 1101 80-81 0.8531603443376916 1101 82-83 0.7585528432312394 1101 84-85 0.7515390998037432 1101 86-87 0.7285928552446386 1101 88-89 0.8975614646159791 1101 90-91 0.8671272798229168 1101 92-93 0.8695687006129624 1101 94-95 0.8194714063751185 1101 96-97 0.7129250508114175 1101 98-99 0.6840477721861262 1101 100-101 0.737659641738901 1101 102-103 0.6649707608121638 1101 104-105 0.7177148608849713 1101 106-107 0.7554622696431359 1101 108-109 0.7316860924089319 1101 110-111 0.7336819248970414 1101 112-113 0.641376439824441 1101 114-115 0.8423219608227228 1101 116-117 0.7681492466820004 1101 118-119 0.8719864189287598 1101 120-121 0.7295979491811977 1101 122-123 0.7302231047288643 1101 124-125 0.7966898731074004 1102 1 0.20901120924914807 1102 2 0.34913335643860677 1102 3 0.3942469185830646 1102 4 0.3932123167041155 1102 5 0.2408932214369912 1102 6 0.28994357123308134 1102 7 0.2887602971161769 1102 8 0.37325616671223827 1102 9 0.39120584539929126 1102 10-11 0.4251619482462985 1102 12-13 0.48002196115412943 1102 14-15 0.4989997096206693 1102 16-17 0.4731466167734055 1102 18-19 0.4575780816394186 1102 20-21 0.4519791125809789 1102 22-23 0.49010704235363534 1102 24-25 0.6131560976383952 1102 26-27 0.7217944346371397 1102 28-29 0.6921688336821177 1102 30-31 0.5840314711822927 1102 32-33 0.7584787005751394 1102 34-35 0.6008233613302281 1102 36-37 0.585819403243562 1102 38-39 0.5990073297704654 1102 40-41 0.5553290078010349 1102 42-43 0.671740728805112 1102 44-45 0.8226026721028035 1102 46-47 0.7337446166831043 1102 48-49 0.7803264238143228 1102 50-51 0.6794584433882278 1102 52-53 0.860084839632627 1102 54-55 0.8567136844263388 1102 56-57 0.835275828117723 1102 58-59 0.7532110853200606 1102 60-61 0.7524862279277258 1102 62-63 0.7450495055556843 1102 64-65 0.6458218280280121 1102 66-67 0.46635627477886743 1102 68-69 0.46337255068654315 1102 70-71 0.6260367913068663 1102 72-73 0.612294688418686 1102 74-75 0.6437139604836766 1102 76-77 0.761229981847201 1102 78-79 0.6361677753614607 1102 80-81 0.4886172845271197 1102 82-83 0.6616971714255264 1102 84-85 0.849822941949423 1102 86-87 0.7393924902540121 1102 88-89 0.6360357707621915 1102 90-91 0.7253232599713897 1102 92-93 0.7373624065890603 1102 94-95 0.81617327839718 1102 96-97 0.6769292952725614 1102 98-99 0.683610733069365 1102 100-101 0.49844503220455394 1102 102-103 0.39658766103429954 1102 104-105 0.7004434548452849 1102 106-107 0.7245169274024903 1102 108-109 0.6791627437437917 1102 110-111 0.5946359790180473 1102 112-113 0.5858460473356537 1102 114-115 0.724927308513454 1102 116-117 0.7779608471080977 1102 118-119 0.5867957897741007 1102 120-121 0.5899217338532452 1102 122-123 0.6989435318174451 1102 124-125 0.5801327586803602 1103 1 0.34539937065494897 1103 2 0.35864005932128507 1103 3 0.4019471529753922 1103 4 0.37046418721021723 1103 5 0.2648103779407194 1103 6 0.28852866495437723 1103 7 0.3415159685845808 1103 8 0.3547850368791785 1103 9 0.4185795655379607 1103 10-11 0.37591325299950284 1103 12-13 0.30698573578030874 1103 14-15 0.3494686410037957 1103 16-17 0.3643573028163871 1103 18-19 0.3608762596174415 1103 20-21 0.4195638193516089 1103 22-23 0.46050982319996336 1103 24-25 0.35014881181432855 1103 26-27 0.5210260462007241 1103 28-29 0.5442160668171709 1103 30-31 0.4327113321819027 1103 32-33 0.5454026188635765 1103 34-35 0.4085713461892624 1103 36-37 0.47596283072091694 1103 38-39 0.5561771734432313 1103 40-41 0.586198804142569 1103 42-43 0.48512161277147925 1103 44-45 0.4987410008867741 1103 46-47 0.5103712663066133 1103 48-49 0.6660843754828036 1103 50-51 0.7150962642732424 1103 52-53 0.649796123290102 1103 54-55 0.7670138667501121 1103 56-57 0.7289360837567003 1103 58-59 0.8028303812040036 1103 60-61 0.8469316412627279 1103 62-63 0.711455542992212 1103 64-65 0.7988583007484102 1103 66-67 0.7764102424485735 1103 68-69 0.7394755801790609 1103 70-71 0.8706259800813569 1103 72-73 0.7201466007640889 1103 74-75 0.6739270306961451 1103 76-77 0.5057927590378242 1103 78-79 0.6147996511247236 1103 80-81 0.5320365659056563 1103 82-83 0.678851809572155 1103 84-85 0.5202777494236486 1103 86-87 0.6274063265701066 1103 88-89 0.6949435595382738 1103 90-91 0.5766546553372898 1103 92-93 0.5781449943960517 1103 94-95 0.5612743488209446 1103 96-97 0.5615612959668077 1103 98-99 0.5163613695675764 1103 100-101 0.5031258565981247 1103 102-103 0.19412505333896846 1103 104-105 0.463993645448884 1103 106-107 0.3615526966524456 1103 108-109 0.41001717525121606 1103 110-111 0.38657170496139415 1103 112-113 0.2977831818968042 1103 114-115 0.34705814059992335 1103 116-117 0.2534005894987317 1103 118-119 0.2993478321231855 1103 120-121 0.24598180888729715 1103 122-123 0.1331120301763633 1103 124-125 0.22063986947528136 1104 1 0.12166694319400762 1104 2 0.1699805151787075 1104 3 0.2005533231131551 1104 4 0.2635809821036261 1104 5 0.15783982861453438 1104 6 0.26674192401586794 1104 7 0.16897644793717603 1104 8 0.24233708557128608 1104 9 0.450745850543818 1104 10-11 0.35984212555070627 1104 12-13 0.38107212673596536 1104 14-15 0.3288893607661336 1104 16-17 0.3898267100855577 1104 18-19 0.33617435311044375 1104 20-21 0.29466294354819667 1104 22-23 0.337331906159001 1104 24-25 0.3948742761857247 1104 26-27 0.4743731581891737 1104 28-29 0.5457060380082055 1104 30-31 0.42830358345408825 1104 32-33 0.4061043648912417 1104 34-35 0.33825632400738215 1104 36-37 0.468206329688563 1104 38-39 0.45069983414875736 1104 40-41 0.5200190501565487 1104 42-43 0.6207803501292872 1104 44-45 0.4851677509525487 1104 46-47 0.5904042732274917 1104 48-49 0.5705160080486564 1104 50-51 0.48806857761833555 1104 52-53 0.48583587368622716 1104 54-55 0.7237577912596294 1104 56-57 0.7448028508065292 1104 58-59 0.6399900391239157 1104 60-61 0.7040643938196851 1104 62-63 0.7002185419747988 1104 64-65 0.7976747656770939 1104 66-67 0.9133531278962117 1104 68-69 0.8283012037133517 1104 70-71 0.9282474632488906 1104 72-73 0.7192101578929524 1104 74-75 0.7074730618312266 1104 76-77 0.629580309477543 1104 78-79 0.6758515889648926 1104 80-81 0.6658115517331176 1104 82-83 0.6521154932038691 1104 84-85 0.5758778825713442 1104 86-87 0.5814773716896653 1104 88-89 0.6526631064432671 1104 90-91 0.5854897947944551 1104 92-93 0.6145711907245435 1104 94-95 0.7710342086355624 1104 96-97 0.8515987474129325 1104 98-99 0.8159606100489896 1104 100-101 0.8104692696215672 1104 102-103 0.32790400935822817 1104 104-105 0.5372797293206482 1104 106-107 0.7613076522354056 1104 108-109 0.6684702882263664 1104 110-111 0.4782070750240166 1104 112-113 0.5603446075550664 1104 114-115 0.653376512787851 1104 116-117 0.6276609584382093 1104 118-119 0.7168454703814611 1104 120-121 0.6288035830072047 1104 122-123 0.6960506449513666 1104 124-125 0.8284433232496049 1105 1 0.15907058562473964 1105 2 0.3037703803599783 1105 3 0.3201652403422486 1105 4 0.3357597192545896 1105 5 0.3538220781912784 1105 6 0.30261915448483734 1105 7 0.32831621438673153 1105 8 0.2176617295312937 1105 9 0.3727334689645687 1105 10-11 0.36412418388592727 1105 12-13 0.39671744000857956 1105 14-15 0.41628197160599 1105 16-17 0.29813372314827546 1105 18-19 0.32813121815744495 1105 20-21 0.23686129605480488 1105 22-23 0.3938852851414225 1105 24-25 0.4418191190500309 1105 26-27 0.556456892671271 1105 28-29 0.6682811346932453 1105 30-31 0.5044905335118841 1105 32-33 0.3702990751308519 1105 34-35 0.41523666853686336 1105 36-37 0.46777888112566046 1105 38-39 0.39855909151597757 1105 40-41 0.5349416816596602 1105 42-43 0.5025032758627361 1105 44-45 0.48043460493112633 1105 46-47 0.4813116635337735 1105 48-49 0.6045914461194712 1105 50-51 0.6332019473006625 1105 52-53 0.6146487312407629 1105 54-55 0.7214334821360353 1105 56-57 0.5938412603908745 1105 58-59 0.6562736087256837 1105 60-61 0.6955563561084332 1105 62-63 0.7877271994199546 1105 64-65 0.7474311906066049 1105 66-67 0.6212259588275657 1105 68-69 0.6380154711829711 1105 70-71 0.6415263877117603 1105 72-73 0.5720224358284618 1105 74-75 0.5911806505833432 1105 76-77 0.409327843640682 1105 78-79 0.4393379646431619 1105 80-81 0.5640226124686762 1105 82-83 0.5205166984512211 1105 84-85 0.5119518601332125 1105 86-87 0.5337931666355189 1105 88-89 0.4953377821677094 1105 90-91 0.5259726223528851 1105 92-93 0.4841069715565105 1105 94-95 0.39486524887160357 1105 96-97 0.4301783656175502 1105 98-99 0.41352234193098525 1105 100-101 0.2527424281850301 1105 102-103 0.08984729265439384 1105 104-105 0.40415554594490644 1105 106-107 0.3222296640046167 1105 108-109 0.5960686679128493 1105 110-111 0.6265768555874587 1105 112-113 0.7375849210405434 1105 114-115 0.666800464807185 1105 116-117 0.4428822206323133 1105 118-119 0.5149160881178041 1105 120-121 0.4692211829587407 1105 122-123 0.28086137232412156 1105 124-125 0.28317450446077785 1106 1 0.11833442611255762 1106 2 0.04039899463033336 1106 3 0.10185865102853953 1106 4 0.04115050313561852 1106 5 0.1239402128666427 1106 6 0.05776180312467005 1106 7 0.11176516942283143 1106 8 0.10517853815594691 1106 9 0.1893476294819436 1106 10-11 0.12833038410493458 1106 12-13 0.18513669287353451 1106 14-15 0.17065647858073874 1106 16-17 0.24224192575710646 1106 18-19 0.16373132350259567 1106 20-21 0.11422095236003571 1106 22-23 0.12365127913026441 1106 24-25 0.10816979189427656 1106 26-27 0.319511764509663 1106 28-29 0.2840522012254354 1106 30-31 0.23157276429929396 1106 32-33 -0.019820469971179477 1106 34-35 0.16961991266798293 1106 36-37 0.10357894253538547 1106 38-39 0.01805135455597906 1106 40-41 0.01910797803732578 1106 42-43 0.14048086547267502 1106 44-45 -0.12722921259045705 1106 46-47 -0.01336220754241424 1106 48-49 -0.12893974220036597 1106 50-51 -0.12513850057430176 1106 52-53 -0.12171912051715594 1106 54-55 -0.2250463383611674 1106 56-57 -0.21558315654955607 1106 58-59 -0.219772977792136 1106 60-61 -0.06113921806420208 1106 62-63 -0.12766769092456798 1106 64-65 -0.2011214162280126 1106 66-67 -0.046172132644635155 1106 68-69 -0.10465904727489317 1106 70-71 -0.24424527793972928 1106 72-73 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-2.503657367157068 2216 74-75 -2.452474882415803 2216 76-77 -2.3745474425967146 2216 78-79 -2.2572724822855648 2216 80-81 -2.293170801732856 2216 82-83 -2.4280635250997484 2216 84-85 -2.514526722727531 2216 86-87 -2.612212912336556 2216 88-89 -2.549411926831489 2216 90-91 -2.6241437346635266 2216 92-93 -2.5486782525314275 2216 94-95 -2.7963615749932345 2216 96-97 -2.80251975309692 2216 98-99 -2.8881165511920415 2216 100-101 -2.706296502698816 2216 102-103 -2.6190049993233373 2216 104-105 -2.7090369450219463 2216 106-107 -2.750108501443197 2216 108-109 -2.882241379582638 2216 110-111 -3.0396915614721145 2216 112-113 -3.027896473095794 2216 114-115 -2.9748846636662023 2216 116-117 -2.9415711915252842 2216 118-119 -2.9729563885761507 2216 120-121 -2.9426078480085955 2216 122-123 -2.7203358664593544 2216 124-125 -2.6905085437033165 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53121.0 3 8201.0 4 5335.0 5 3605.0 6 3837.0 7 4059.0 8 4933.0 9 6170.0 10 7981.0 11 9596.0 12 11368.0 13 12635.0 14 13251.0 15 13112.0 16 12947.0 17 13319.0 18 13431.0 19 14065.0 20 14477.0 21 15649.0 22 17016.0 23 19234.0 24 22153.0 25 24707.0 26 27665.0 27 29225.0 28 33735.0 29 40916.0 30 47980.0 31 56438.0 32 69381.0 33 83451.0 34 97734.0 35 114496.0 36 156491.0 37 235538.0 38 100661.0 39 588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.66920654171313 15.725217445512879 9.414515825102825 22.19106018767117 2 12.79654053747751 22.385782487356387 37.17137231013331 27.646304665032794 3 22.60758035993147 32.58108585677954 25.787062382125665 19.024271401163322 4 13.410599895891984 18.517275202815874 33.08581121037964 34.9863136909125 5 11.511818061417232 42.27943077264122 31.16444007538516 15.044311090556386 6 30.30096340587563 27.81460846850905 22.59123741204902 19.293190713566304 7 26.793147648487682 32.49222772242169 21.36544222311925 19.349182405971373 8 30.252957427215417 32.210172132889426 21.588226960402352 15.94864347949281 9 32.92414833812137 16.34262719579456 19.03960666097534 31.69361780510873 10-11 23.990448073354717 30.532122090654738 23.77485311131941 21.702576724671136 12-13 27.716479311457654 27.199704133103264 22.977684933637182 22.106131621801907 14-15 28.666407897374363 25.185807448730746 22.643930384449476 23.503854269445416 16-17 23.89921336587394 27.9850996033715 29.524899337677514 18.590787693077047 18-19 26.44933720404002 26.997895852116155 27.852019833240437 18.700747110603388 20-21 22.247162360774176 28.295468631027216 29.3292723956581 20.128096612540507 22-23 21.10721361076546 30.97106557612222 21.681708231541982 26.240012581570337 24-25 21.228172386131618 32.739163002537595 21.224170324893 24.808494286437792 26-27 24.539989862757604 26.322047652123278 21.876254094124757 27.26170839099436 28-29 23.858320370165732 27.452163786435 25.076999798822975 23.612516044576296 30-31 27.473464437174016 28.60403315050209 24.10974267128208 19.812759741041813 32-33 19.937132582664916 29.050145160399055 27.195648446875825 23.817073810060204 34-35 17.784600540728366 30.63442604195409 27.74514571789961 23.835827699417937 36-37 24.939961018476392 27.765891343871207 24.795258487155746 22.49888915049665 38-39 27.977470032595193 26.36395488343945 23.627763601680368 22.03081148228499 40-41 22.53428218246625 30.77481678244169 23.822617729379818 22.86828330571224 42-43 26.066741367997015 28.490653249684833 22.81146455426594 22.631140828052214 44-45 28.061553603608385 26.022984354919227 23.42757910165585 22.487882939816537 46-47 23.709158478504946 27.32612423392549 29.863743276655004 19.10097401091456 48-49 26.19600123213318 26.717144000254923 27.701540520540863 19.38531424707103 50-51 21.224351803339044 28.605509848624305 29.2595831150257 20.910555233010946 52-53 20.433558729972184 31.09992191001173 22.001199314960356 26.46532004505573 54-55 20.97702400611702 32.3279189919661 21.374736495563567 25.320320506353312 56-57 24.176836573510897 26.438843764677067 21.986500371096454 27.39781929071558 58-59 23.78566910622429 27.240704237765375 24.937217700874157 24.036408955136185 60-61 26.814853202867443 28.539519064564967 24.24932379405958 20.396303938508005 62-63 19.815444390480817 29.130978694121097 26.872388447504164 24.18118846789392 64-65 17.571182110006156 30.335724155803877 27.70925361234405 24.383840121845914 66-67 24.321152679316725 27.760223331263617 24.786626744952496 23.131997244467165 68-69 27.12158650816689 26.371733420182526 23.850794032894132 22.65588603875645 70-71 22.135214383177047 30.21169599670316 23.923304544981416 23.729785075138377 72-73 25.467585197018106 28.23443483334466 23.042635329571976 23.255344640065257 74-75 27.386893039914856 25.684101400599495 23.587026025700673 23.341979533784976 76-77 23.256739366325206 27.057619834067054 29.758358159549818 19.927282640057918 78-79 25.585364256344995 26.624458159947466 27.618027764158644 20.1721498195489 80-81 20.984497781654788 28.39439388124737 28.915205825333462 21.70590251176438 82-83 20.16663032057255 30.57783917278178 22.313870235947515 26.941660270698158 84-85 20.644708837744112 31.78141908587952 21.73372633594218 25.84014574043419 86-87 23.63965423483029 26.08087019078262 22.434234377124213 27.845241197262883 88-89 23.308078510721824 26.632291883116764 25.232863435281256 24.82676617088015 90-91 26.081997378949893 28.023571042706408 24.48646321785939 21.407968360484304 92-93 19.66147456014139 28.538872115286573 26.754619506723916 25.04503381784812 94-95 17.65316060528925 29.59850005292723 27.70490892091114 25.043430420872376 96-97 24.036599708298073 27.156805180841392 25.07914564816614 23.727449462694395 98-99 26.42466953992995 26.073572853712307 24.070450028606434 23.431307577751305 100-101 21.862166254052575 29.106471418881792 24.201417477122828 24.829944849942805 102-103 24.865433922537484 27.48671319172058 23.685433752738017 23.962419133003923 104-105 26.406166202767544 25.255875623150715 24.039113275816444 24.298844898265294 106-107 22.62295569200591 26.70647661115636 29.595805342887534 21.0747623539502 108-109 24.59443105699515 26.51132507439765 27.562552414517892 21.331691454089306 110-111 20.29292182852645 28.306846285938363 28.973623374288454 22.42660851124673 112-113 19.68875335363059 30.177526100898582 22.743145074670814 27.39057547080001 114-115 20.0538551862424 31.776400118058156 22.27370948209416 25.896035213605284 116-117 22.75040624777771 26.55457659835902 22.79470167278913 27.900315481074145 118-119 22.329517190346284 27.040916582430636 25.192421058594643 25.43714516862844 120-121 24.675540341204023 28.270143337981203 24.737787307992186 22.316529012822592 122-123 19.384057393976263 28.77233701512959 26.347122284971668 25.496483305922478 124-125 17.757357286791034 29.913628542571015 27.067220818048042 25.261793352589905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6229.0 1 3632.0 2 1616.0 3 2197.0 4 2015.5 5 1597.5 6 1269.0 7 1096.0 8 995.5 9 938.5 10 863.0 11 816.0 12 823.0 13 859.5 14 905.5 15 992.0 16 1085.5 17 1153.0 18 1324.0 19 1503.0 20 1641.0 21 1997.0 22 2487.5 23 3043.0 24 3692.0 25 4482.5 26 5618.5 27 6749.5 28 7690.5 29 8985.0 30 10743.5 31 12361.5 32 13949.5 33 15268.5 34 16992.0 35 18663.0 36 20066.5 37 21683.0 38 22473.0 39 22538.5 40 22559.5 41 23686.5 42 24713.5 43 25897.0 44 27756.5 45 29518.5 46 32800.5 47 41013.0 48 52608.0 49 85388.0 50 202161.5 51 224864.5 52 110214.0 53 50971.0 54 34652.0 55 27682.5 56 23208.5 57 19995.0 58 18569.0 59 16969.0 60 14467.5 61 12048.5 62 10157.0 63 7987.0 64 6140.0 65 4935.0 66 3686.5 67 2928.0 68 2273.5 69 1579.0 70 1290.0 71 1086.0 72 996.0 73 928.5 74 725.0 75 563.0 76 395.5 77 252.5 78 199.0 79 160.0 80 110.0 81 73.0 82 65.0 83 51.5 84 38.5 85 37.0 86 34.0 87 31.5 88 22.0 89 13.0 90 12.5 91 10.5 92 7.0 93 6.0 94 9.5 95 7.0 96 4.5 97 7.5 98 6.0 99 4.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4852305356147088 2 0.4738100290376955 3 0.4589351717059064 4 0.4586531838891901 5 0.4618960437814284 6 0.4677472909782933 7 0.4533659123257579 8 0.479449785372023 9 0.4931966914369465 10-11 0.4975675025960503 12-13 0.4973912602106026 14-15 0.47162462345814354 16-17 0.4741977622856805 18-19 0.4602041168811301 20-21 0.4706376660996362 22-23 0.4873101957629921 24-25 0.47433875619403865 26-27 0.4846313115041865 28-29 0.48001376100545573 30-31 0.4934081822994837 32-33 0.49058830413231996 34-35 0.48547727495433557 36-37 0.44367258112613245 38-39 0.4029605900877053 40-41 0.39700359745957176 42-43 0.40634444388830177 44-45 0.4396190062608345 46-47 0.4476909075143408 48-49 0.44525876259516206 50-51 0.4315118565302386 52-53 0.4249556397915828 54-55 0.42640082735225426 56-57 0.4085650979449433 58-59 0.4146278360043454 60-61 0.424708900451956 62-63 0.42488514283740375 64-65 0.43887878824195403 66-67 0.4279165118671048 68-69 0.42939694790486577 70-71 0.43990099407755084 72-73 0.4402534788484463 74-75 0.44554075041187846 76-77 0.43591791616643205 78-79 0.42781076643583615 80-81 0.4361646555060589 82-83 0.4337677590639697 84-85 0.4319348382553132 86-87 0.43799757631471536 88-89 0.4252023791312096 90-91 0.4293264509506867 92-93 0.43912552758158085 94-95 0.43598841312061115 96-97 0.4058862136861377 98-99 0.37768743201449984 100-101 0.37761693506032074 102-103 0.357349060733831 104-105 0.3608739084427857 106-107 0.3785686439417385 108-109 0.3867462906265135 110-111 0.40430003221710803 112-113 0.39947099085584004 114-115 0.3974618276617358 116-117 0.37099022136748583 118-119 0.4440250658970279 120-121 0.4500525554793405 122-123 0.404617268510914 124-125 0.398307791111885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1418501.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.41962771998625 #Duplication Level Percentage of deduplicated Percentage of total 1 89.1287667138751 48.50354303710585 2 7.711371318348132 8.392999127101698 3 1.59184500161638 2.59882837127653 4 0.5575047841520095 1.213568112226546 5 0.2750701708300093 0.7484608146721065 6 0.17641618190748276 0.576030176591195 7 0.10481282351883976 0.3992712385323116 8 0.07428184155055133 0.32339121308288077 9 0.054760357435075534 0.26820344388871725 >10 0.2827103627404318 2.7687320564826443 >50 0.01899553044387339 0.7072276419858786 >100 0.01660999740522472 1.8514365517664788 >500 0.0021092049775061883 0.8625161158590929 >1k 0.0018455543553179145 2.4070534172353106 >5k 0.0014500784220355045 5.54275826828754 >10k+ 0.0014500784220355045 22.835980413905208 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 85175 6.00457807220439 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 54300 3.8279846119248417 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 36895 2.600985124437699 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 31533 2.222980456129393 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 21474 1.5138515940418795 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 20818 1.4676055921003934 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 16295 1.148747868348348 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15478 1.0911518567840277 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 14924 1.0520965441688093 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11404 0.8039472654583959 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10025 0.7067319656454243 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8412 0.5930203785545446 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8409 0.5928088876920072 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8334 0.5875216161285752 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8316 0.5862526709533514 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7677 0.5412051172329099 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7515 0.5297846106558965 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7453 0.5254137994967928 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5493 0.38723976930576715 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5281 0.37229441501979904 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5271 0.3715894454780081 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5102 0.35967546022174113 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4744 0.33443755062562525 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 4475 0.3154738699514487 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4376 0.30849467148771836 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 3829 0.26993283755175357 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3395 0.23933715943802647 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2917 0.2056396153404192 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1598 0.1126541327781933 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1485 0.10468797695595562 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.049695417909469E-5 0.0 0.0 0.0 0.0 3 7.049695417909469E-5 0.0 0.0 0.0 0.0 4 7.049695417909469E-5 0.0 0.0 0.0 0.0 5 7.049695417909469E-5 0.0 0.0 0.0 0.0 6 7.049695417909469E-5 0.0 0.0 0.0 0.0 7 7.049695417909469E-5 0.0 0.0 0.0 0.0 8 7.049695417909469E-5 0.0 0.0 0.0 0.0 9 7.049695417909469E-5 0.0 0.0 7.049695417909469E-5 0.0 10-11 7.049695417909469E-5 0.0 0.0 4.582302021641155E-4 0.0 12-13 7.049695417909469E-5 0.0 0.0 9.517088814177783E-4 0.0 14-15 7.049695417909469E-5 0.0 0.0 0.0020796601482832933 0.0 16-17 7.049695417909469E-5 0.0 0.0 0.0035248477089547346 0.0 18-19 7.049695417909469E-5 0.0 0.0 0.005252023086342554 0.0 20-21 7.049695417909469E-5 0.0 0.0 0.006943949986640828 0.0 22-23 7.049695417909469E-5 0.0 0.0 0.010821282466491035 0.0 24-25 7.049695417909469E-5 0.0 0.0 0.014522372560893507 0.0 26-27 7.049695417909469E-5 0.0 0.0 0.01903417762835557 0.0 28-29 7.049695417909469E-5 0.0 0.0 0.02513216416484726 0.0 30-31 7.049695417909469E-5 0.0 0.0 0.031899871766040346 0.0 32-33 7.049695417909469E-5 0.0 0.0 0.042016184690740435 0.0 34-35 7.049695417909469E-5 0.0 0.0 0.05777225394976809 0.0 36-37 7.049695417909469E-5 0.0 0.0 0.07934432192857108 0.0 38-39 7.049695417909469E-5 0.0 0.0 0.11709544089147628 0.0 40-41 7.049695417909469E-5 0.0 0.0 0.18635869837243682 0.0 42-43 7.049695417909469E-5 0.0 0.0 0.23461386350802715 0.0 44-45 7.049695417909469E-5 0.0 0.0 0.2865701187380199 0.0 46-47 7.049695417909469E-5 0.0 0.0 0.3539652069332344 0.0 48-49 7.049695417909469E-5 0.0 0.0 0.4123014365164353 0.0 50-51 7.049695417909469E-5 0.0 0.0 0.47796934933426205 0.0 52-53 7.049695417909469E-5 0.0 0.0 0.5639403849556679 0.0 54-55 1.4099390835818938E-4 0.0 0.0 0.6531895289464018 0.0 56-57 1.4099390835818938E-4 0.0 0.0 0.7580537482878051 0.0 58-59 1.4099390835818938E-4 0.0 0.0 0.8795904972925644 0.0 60-61 1.4099390835818938E-4 0.0 0.0 0.9897772366744895 0.0 62-63 1.4099390835818938E-4 0.0 0.0 1.0941479773366392 0.0 64-65 1.4099390835818938E-4 0.0 0.0 1.2243558517054272 0.0 66-67 3.5248477089547346E-4 0.0 0.0 1.3756775638508538 0.0 68-69 3.5248477089547346E-4 0.0 0.0 1.5598156081666492 0.0 70-71 3.5248477089547346E-4 0.0 0.0 1.8454340180232514 0.0 72-73 3.5248477089547346E-4 0.0 0.0 2.0591807830942663 0.0 74-75 3.5248477089547346E-4 0.0 0.0 2.27458422658849 0.0 76-77 3.5248477089547346E-4 0.0 0.0 2.523931953519948 0.0 78-79 3.5248477089547346E-4 0.0 0.0 2.7600262530657362 0.0 80-81 3.5248477089547346E-4 0.0 0.0 2.9995749033663 0.0 82-83 3.5248477089547346E-4 0.0 0.0 3.308281065716556 0.0 84-85 3.5248477089547346E-4 0.0 0.0 3.6057429638752456 0.0 86-87 3.5248477089547346E-4 0.0 0.0 3.916987016575949 0.0 88-89 3.5248477089547346E-4 0.0 0.0 4.3015831501 0.0 90-91 3.5248477089547346E-4 0.0 0.0 4.630839174593461 0.0 92-93 3.5248477089547346E-4 0.0 0.0 4.960165696041102 0.0 94-95 3.5248477089547346E-4 0.0 0.0 5.341342727287468 0.0 96-97 4.2298172507456816E-4 0.0 0.0 5.72967519938301 0.0 98-99 4.2298172507456816E-4 0.0 0.0 6.183428844956754 0.0 100-101 4.2298172507456816E-4 0.0 0.0 6.882723381936284 0.0 102-103 4.2298172507456816E-4 0.0 0.0 7.4232235296274025 0.0 104-105 4.2298172507456816E-4 0.0 0.0 7.946557668975911 0.0 106-107 4.2298172507456816E-4 0.0 0.0 8.487410301437926 0.0 108-109 4.2298172507456816E-4 7.049695417909469E-5 0.0 8.958365203831368 0.0 110-111 4.934786792536629E-4 7.049695417909469E-5 0.0 9.44638036913615 0.0 112-113 4.934786792536629E-4 7.049695417909469E-5 0.0 10.070666146869124 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 230 0.0 67.2761 2 ATAACGC 270 0.0 57.31333 3 AGTACTC 1630 0.0 51.491924 5 AAGTACT 1810 0.0 45.049305 4 GTATAAC 475 0.0 42.596104 1 AAAAAGT 2100 0.0 38.53933 1 GTACAAA 1010 0.0 37.119637 1 AAAGTAC 2275 0.0 35.841423 3 AAAAGTA 2285 0.0 35.161133 2 TAACGCA 475 0.0 33.831112 4 TATCACG 145 1.3382282E-4 24.626266 2 ATGGGGG 2375 0.0 23.810452 5 CATGGGG 6555 0.0 23.153334 4 ATCACGC 160 2.6064244E-4 22.319136 3 TACAACG 295 4.7548383E-9 22.191465 2 CATGGGT 4000 0.0 21.872751 4 GTACTTG 1010 0.0 21.211222 1 ATGGGAG 2175 0.0 21.073618 5 ATGGGTG 1215 0.0 21.06686 5 GAACAAA 1130 0.0 19.485332 1 >>END_MODULE