##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364144_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1418501 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05022132518764 33.0 31.0 34.0 30.0 34.0 2 31.878264449584456 33.0 31.0 34.0 30.0 34.0 3 32.11940069129313 34.0 31.0 34.0 30.0 34.0 4 35.57390512942888 37.0 35.0 37.0 33.0 37.0 5 35.70845279629694 37.0 35.0 37.0 33.0 37.0 6 35.85343048753579 37.0 35.0 37.0 35.0 37.0 7 35.83103149028446 37.0 35.0 37.0 35.0 37.0 8 35.890107232917 37.0 35.0 37.0 35.0 37.0 9 37.5525375026172 39.0 37.0 39.0 35.0 39.0 10-11 37.49390659576553 39.0 37.0 39.0 34.0 39.0 12-13 37.47759148565986 39.0 37.0 39.0 34.0 39.0 14-15 38.76153911770242 40.0 38.0 41.0 34.0 41.0 16-17 38.736982913653215 40.0 38.0 41.0 34.0 41.0 18-19 38.79713796465424 40.0 38.0 41.0 34.0 41.0 20-21 38.83015380320494 40.0 38.0 41.0 35.0 41.0 22-23 38.79205689668178 40.0 38.0 41.0 34.5 41.0 24-25 38.7645162040774 40.0 38.0 41.0 34.5 41.0 26-27 38.56057098303068 40.0 38.0 41.0 34.0 41.0 28-29 38.385532333075545 40.0 38.0 41.0 34.0 41.0 30-31 38.233233533145196 40.0 38.0 41.0 34.0 41.0 32-33 37.87368602489529 40.0 37.0 41.0 33.5 41.0 34-35 37.61074507525902 40.0 37.0 41.0 33.0 41.0 36-37 37.486394440328205 40.0 37.0 41.0 33.0 41.0 38-39 37.364977183660784 40.0 37.0 41.0 32.5 41.0 40-41 37.08176518733508 40.0 36.0 41.0 31.0 41.0 42-43 36.885899269722046 40.0 36.0 41.0 31.0 41.0 44-45 36.61633266384726 40.0 36.0 41.0 30.5 41.0 46-47 36.28230681543404 39.0 35.0 41.0 30.0 41.0 48-49 35.9347205253997 39.0 35.0 41.0 29.0 41.0 50-51 35.782415028258704 39.0 35.0 41.0 28.0 41.0 52-53 35.66203865911973 39.0 35.0 41.0 28.0 41.0 54-55 35.459885470648246 39.0 35.0 41.0 27.0 41.0 56-57 35.166775349471024 39.0 35.0 41.0 26.0 41.0 58-59 34.95230599061968 38.5 35.0 40.0 26.0 41.0 60-61 34.77652113040456 38.0 35.0 40.0 26.0 41.0 62-63 34.37895214737247 38.0 34.0 40.0 25.5 41.0 64-65 34.101732039667226 37.5 34.0 40.0 24.5 41.0 66-67 33.62214443274978 37.0 34.0 39.0 23.0 41.0 68-69 33.29625499030314 36.0 33.5 39.0 22.0 41.0 70-71 32.750546880122045 35.5 33.0 39.0 22.0 40.0 72-73 32.42071736290633 35.0 33.0 37.5 21.5 39.0 74-75 32.1145748222948 35.0 33.0 37.0 20.5 39.0 76-77 31.594910401896087 35.0 32.0 36.5 20.0 39.0 78-79 31.16690717877534 35.0 32.0 36.0 18.0 37.0 80-81 30.919925682110904 35.0 32.0 36.0 16.5 37.0 82-83 30.724363253885613 35.0 32.0 35.0 14.0 36.5 84-85 30.50329502763833 35.0 32.0 35.0 11.0 36.0 86-87 30.22085391550658 35.0 31.0 35.0 7.0 36.0 88-89 29.998513219236365 34.0 31.0 35.0 7.0 35.5 90-91 29.846347658549412 34.0 31.0 35.0 3.5 35.0 92-93 29.612382719504605 34.0 31.0 35.0 2.0 35.0 94-95 29.535502266124592 34.0 31.0 35.0 2.0 35.0 96-97 29.421692335782634 34.0 30.5 35.0 2.0 35.0 98-99 29.305293757283216 34.0 30.0 35.0 2.0 35.0 100-101 29.003915400835105 34.0 30.0 35.0 2.0 35.0 102-103 27.57423822753738 32.5 26.0 34.5 2.0 35.0 104-105 28.50133803219032 34.0 29.0 35.0 2.0 35.0 106-107 28.538890349742438 34.0 29.0 35.0 2.0 35.0 108-109 28.42100781035755 34.0 29.0 35.0 2.0 35.0 110-111 28.407747685761237 34.0 29.0 35.0 2.0 35.0 112-113 28.340164018213592 34.0 29.0 35.0 2.0 35.0 114-115 28.27754016387722 34.0 29.0 35.0 2.0 35.0 116-117 28.011297136907203 34.0 29.0 35.0 2.0 35.0 118-119 27.808868305344866 34.0 29.0 35.0 2.0 35.0 120-121 27.62096924852362 34.0 29.0 35.0 2.0 35.0 122-123 27.23873476296457 34.0 27.0 35.0 2.0 35.0 124-125 26.162036544211105 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29255511745743945 1101 2 0.6371063649644135 1101 3 0.4936807689074669 1101 4 0.5670658589740043 1101 5 0.19851916011991477 1101 6 0.2491982848564902 1101 7 0.23148580617824877 1101 8 0.1502267374343731 1101 9 0.23014722313852332 1101 10-11 0.31249721764881144 1101 12-13 0.32773856579094485 1101 14-15 0.4175525122006434 1101 16-17 0.3982714874994997 1101 18-19 0.2851802640360006 1101 20-21 0.2834562831902403 1101 22-23 0.41175727572935017 1101 24-25 0.36384149716725744 1101 26-27 0.3073627070696219 1101 28-29 0.4190865287286627 1101 30-31 0.3219449305503588 1101 32-33 0.3180412456847108 1101 34-35 0.3477122613584598 1101 36-37 0.2739549556157215 1101 38-39 0.22116861569985957 1101 40-41 0.2631380981178637 1101 42-43 0.2478543470908292 1101 44-45 0.34977090854936677 1101 46-47 0.4637618635713352 1101 48-49 0.4866786130495413 1101 50-51 0.4992254713000932 1101 52-53 0.5393028850816108 1101 54-55 0.5248222057191327 1101 56-57 0.5328058048040631 1101 58-59 0.43371580828532785 1101 60-61 0.3604361955596431 1101 62-63 0.39637328651622994 1101 64-65 0.48475221931601453 1101 66-67 0.4783686101114526 1101 68-69 0.4118599178528015 1101 70-71 0.4098709360535864 1101 72-73 0.45589655892530345 1101 74-75 0.3862562497335489 1101 76-77 0.3468173359379563 1101 78-79 0.4371910321314161 1101 80-81 0.4622934336938833 1101 82-83 0.4713919646855764 1101 84-85 0.44914357592057996 1101 86-87 0.45488747367995686 1101 88-89 0.3850671351536583 1101 90-91 0.4013024131036538 1101 92-93 0.41660552384519534 1101 94-95 0.3745079177221129 1101 96-97 0.4695414182414588 1101 98-99 0.45324321699335 1101 100-101 0.39225929861832043 1101 102-103 0.30313221349491215 1101 104-105 0.3365979954646967 1101 106-107 0.32930312802674777 1101 108-109 0.33297176415815954 1101 110-111 0.28194454698605753 1101 112-113 0.39873544115765114 1101 114-115 0.35938349278127646 1101 116-117 0.3657553108400293 1101 118-119 0.32286658797250567 1101 120-121 0.2867369099271926 1101 122-123 0.38085138667431195 1101 124-125 0.36612651245775396 1102 1 0.2899473337432852 1102 2 0.6420742011975769 1102 3 0.3943475787077162 1102 4 0.4649275258746428 1102 5 0.24380442259111135 1102 6 0.2879595463037319 1102 7 0.23582853402901094 1102 8 0.14202972507258949 1102 9 0.1826146258420991 1102 10-11 0.3018631496548494 1102 12-13 0.1945929576521479 1102 14-15 0.3557821837916535 1102 16-17 0.34665049971264494 1102 18-19 0.3024611664381709 1102 20-21 0.2123237800316673 1102 22-23 0.19780154529392036 1102 24-25 0.2482837716699393 1102 26-27 0.23512201969812452 1102 28-29 0.3942279419525434 1102 30-31 0.3561766335371672 1102 32-33 0.4199524918551987 1102 34-35 0.4861248411485448 1102 36-37 0.5218998189093895 1102 38-39 0.431172827130176 1102 40-41 0.5141566862152018 1102 42-43 0.5338971715567595 1102 44-45 0.5605366869620667 1102 46-47 0.6457499477008497 1102 48-49 0.6555485046258553 1102 50-51 0.46397889618386046 1102 52-53 0.4751609433645143 1102 54-55 0.48930890668280824 1102 56-57 0.5581712083168924 1102 58-59 0.6381495773503119 1102 60-61 0.5305971795479536 1102 62-63 0.5451435075750339 1102 64-65 0.5117532680447354 1102 66-67 0.4711788315005734 1102 68-69 0.610410690361249 1102 70-71 0.6245339390511333 1102 72-73 0.7391146284386707 1102 74-75 0.6867275583322936 1102 76-77 0.6253252096611064 1102 78-79 0.7478886844654582 1102 80-81 0.6277163521325129 1102 82-83 0.5948281615162685 1102 84-85 0.4712333537056992 1102 86-87 0.5560949568135918 1102 88-89 0.6120330886297403 1102 90-91 0.5920963090070046 1102 92-93 0.7265959036171203 1102 94-95 0.6961910034618803 1102 96-97 0.6896014517677465 1102 98-99 0.5920746689458873 1102 100-101 0.5143825201147791 1102 102-103 0.49376198748148425 1102 104-105 0.5867736781705801 1102 106-107 0.6418998464141836 1102 108-109 0.6734142946497386 1102 110-111 0.6181015342444169 1102 112-113 0.6396715760555445 1102 114-115 0.5566342958432315 1102 116-117 0.5834185450533589 1102 118-119 0.6366707894935786 1102 120-121 0.5646576607178666 1102 122-123 0.68263876724723 1102 124-125 0.5961354307807767 1103 1 0.20165550502618146 1103 2 0.5581448953652703 1103 3 0.32107786235555125 1103 4 0.5176435799672419 1103 5 0.11718100105007778 1103 6 0.1970523419363488 1103 7 0.20719460403145007 1103 8 0.18839798555956122 1103 9 0.34268936643213266 1103 10-11 0.3105981521723393 1103 12-13 0.3294633962563296 1103 14-15 0.47609900122095183 1103 16-17 0.4420941032253012 1103 18-19 0.3422073511171888 1103 20-21 0.25345164689381505 1103 22-23 0.3098045830452989 1103 24-25 0.34574188729985167 1103 26-27 0.3472757711701604 1103 28-29 0.3307770463273627 1103 30-31 0.26997236802444746 1103 32-33 0.2921108884121324 1103 34-35 0.4620551891903304 1103 36-37 0.4229579382285422 1103 38-39 0.39688147298311804 1103 40-41 0.5879136543666093 1103 42-43 0.48230245689200046 1103 44-45 0.4870209865331532 1103 46-47 0.6443243510191508 1103 48-49 0.6856433515581486 1103 50-51 0.6664354250225841 1103 52-53 0.6158800804854891 1103 54-55 0.6257775605346865 1103 56-57 0.5717478105043554 1103 58-59 0.43724996945751826 1103 60-61 0.501544227676149 1103 62-63 0.6342386886348237 1103 64-65 0.6468734638090794 1103 66-67 0.545180526034251 1103 68-69 0.5526883834309757 1103 70-71 0.5933564267959497 1103 72-73 0.573571851524747 1103 74-75 0.4687926884596578 1103 76-77 0.39436576066544404 1103 78-79 0.4523593061899476 1103 80-81 0.4123810319214023 1103 82-83 0.30309974554472063 1103 84-85 0.257799562725296 1103 86-87 0.23588709764854343 1103 88-89 0.3340324077978032 1103 90-91 0.24480882059351572 1103 92-93 0.25114924390690163 1103 94-95 0.2827327503240369 1103 96-97 0.13950590891117542 1103 98-99 0.17172687030948097 1103 100-101 0.20618784410316238 1103 102-103 0.11697967720574809 1103 104-105 0.1884412971914493 1103 106-107 0.18907460348001592 1103 108-109 0.33157126078883437 1103 110-111 0.3582187807152195 1103 112-113 0.4028787304924606 1103 114-115 0.34513756396231443 1103 116-117 0.31889175469384057 1103 118-119 0.36410345357973384 1103 120-121 0.3518297640483681 1103 122-123 0.34000411952535714 1103 124-125 0.21231128916773656 1104 1 0.23733143958320824 1104 2 0.513528506789644 1104 3 0.007436680350046743 1104 4 0.3679736178324262 1104 5 0.15383297591750988 1104 6 0.1673149784822172 1104 7 0.18000882015991237 1104 8 0.16349903216480755 1104 9 0.33330843008120326 1104 10-11 0.24599487776710305 1104 12-13 0.2263891722668845 1104 14-15 0.18921576268117235 1104 16-17 0.3390212374593631 1104 18-19 0.20504620959928133 1104 20-21 0.14087403748002458 1104 22-23 0.1747401207127055 1104 24-25 0.16525581880735984 1104 26-27 0.32458779669305216 1104 28-29 0.3912975008599844 1104 30-31 0.3780941684938952 1104 32-33 0.3616054391149319 1104 34-35 0.3124848997654439 1104 36-37 0.320951695833287 1104 38-39 0.3612094041391529 1104 40-41 0.396594290675516 1104 42-43 0.4420079355268314 1104 44-45 0.3727446574226434 1104 46-47 0.3993191135003329 1104 48-49 0.32087947244672677 1104 50-51 0.2447970856744064 1104 52-53 0.2574377915069306 1104 54-55 0.4446761123132035 1104 56-57 0.5591280334745079 1104 58-59 0.48387005114735615 1104 60-61 0.44471793213465105 1104 62-63 0.4173821109777691 1104 64-65 0.42179232040219716 1104 66-67 0.5030934630714015 1104 68-69 0.5451228695199433 1104 70-71 0.49966219884884566 1104 72-73 0.5801431458260851 1104 74-75 0.5149784520623157 1104 76-77 0.524730560694195 1104 78-79 0.4243744689793054 1104 80-81 0.4195229890546166 1104 82-83 0.4463004890989595 1104 84-85 0.5041820388837763 1104 86-87 0.5001380488751543 1104 88-89 0.4744740770085265 1104 90-91 0.444977323891802 1104 92-93 0.46790583541783803 1104 94-95 0.40669415337310255 1104 96-97 0.4720323530005359 1104 98-99 0.49114395003281075 1104 100-101 0.551486499691741 1104 102-103 0.3572712522706851 1104 104-105 0.43363198445881324 1104 106-107 0.5191961561259113 1104 108-109 0.4926280464421495 1104 110-111 0.3346277965610902 1104 112-113 0.41264456649052406 1104 114-115 0.43617719629747853 1104 116-117 0.48748657635178105 1104 118-119 0.4613749288173352 1104 120-121 0.41820687835834036 1104 122-123 0.3909830538966226 1104 124-125 0.42886456506701975 1105 1 0.18719869209079576 1105 2 0.5417646720694869 1105 3 0.2103493887427348 1105 4 0.3857635419579992 1105 5 0.15167659666983724 1105 6 0.15152199743936023 1105 7 0.20198235744054926 1105 8 0.19021451617943796 1105 9 0.2477935433688856 1105 10-11 0.16874345082365494 1105 12-13 0.15185880284980868 1105 14-15 0.24324937485382492 1105 16-17 0.28559528378958987 1105 18-19 0.17848033769836036 1105 20-21 0.21306311035156256 1105 22-23 0.18438375734724133 1105 24-25 0.14075504434465103 1105 26-27 0.22664466648835457 1105 28-29 0.2716040235388064 1105 30-31 0.2753225171036249 1105 32-33 0.20157184904638825 1105 34-35 0.3783825888211183 1105 36-37 0.4026610930295007 1105 38-39 0.23744590425540224 1105 40-41 0.22485125019714758 1105 42-43 0.19246946126748554 1105 44-45 0.23921584593635714 1105 46-47 0.20071148856296617 1105 48-49 0.21168237987469496 1105 50-51 0.20401980264676922 1105 52-53 0.21122371478763569 1105 54-55 0.2287246365769704 1105 56-57 0.3140378077505588 1105 58-59 0.3768208441120251 1105 60-61 0.23235325844051147 1105 62-63 0.09323426555593528 1105 64-65 0.12249287758962879 1105 66-67 0.23306416693191778 1105 68-69 0.1580056186497032 1105 70-71 0.1662879319686752 1105 72-73 0.2195579966806065 1105 74-75 0.06370636022708709 1105 76-77 0.06430879780094756 1105 78-79 0.1065562802396336 1105 80-81 0.02154391919578913 1105 82-83 0.07013091533179505 1105 84-85 0.06936431187450864 1105 86-87 0.12174527184509643 1105 88-89 0.07606274889503695 1105 90-91 0.15210976929464337 1105 92-93 0.1324291192379512 1105 94-95 0.11043464041681972 1105 96-97 0.09105124546974253 1105 98-99 0.23217745659691502 1105 100-101 0.21402778117373344 1105 102-103 0.032548188181849014 1105 104-105 0.14334856467382906 1105 106-107 0.0441871098120572 1105 108-109 0.180725062222848 1105 110-111 0.18854528026135142 1105 112-113 0.16409780973730292 1105 114-115 0.2621847550862384 1105 116-117 0.3320154394143273 1105 118-119 0.3598547182879166 1105 120-121 0.26311724207746323 1105 122-123 0.14552442625108952 1105 124-125 0.2559309723787351 1106 1 0.23178460912608756 1106 2 0.46314919515388553 1106 3 0.31600137199401956 1106 4 0.5367272503067326 1106 5 0.20236375321637468 1106 6 0.11732476736756325 1106 7 0.05103549932198348 1106 8 0.028760395403644168 1106 9 0.14483218822761046 1106 10-11 0.09708046208376686 1106 12-13 0.1285491097653093 1106 14-15 0.1267430350067329 1106 16-17 0.2301866303434963 1106 18-19 0.09026002156307555 1106 20-21 0.1090785717899152 1106 22-23 0.009749532158274121 1106 24-25 -0.009679233932949671 1106 26-27 0.01857864853474922 1106 28-29 0.15616049635710283 1106 30-31 0.06920696529811465 1106 32-33 0.05858266814232138 1106 34-35 0.11209444905325228 1106 36-37 0.02515612949256507 1106 38-39 -0.053746355622571684 1106 40-41 0.07332877989516362 1106 42-43 0.04515455584512296 1106 44-45 0.07194080274984316 1106 46-47 0.20027639897798366 1106 48-49 0.2886890758649159 1106 50-51 0.13654950481791417 1106 52-53 0.14403217385281408 1106 54-55 -0.012801153844449686 1106 56-57 0.0363582883108009 1106 58-59 0.06341717250927559 1106 60-61 0.03833742005627272 1106 62-63 0.05358754623334505 1106 64-65 0.11169240012484494 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120-121 -2.60766173068971 2216 122-123 -2.667516003806522 2216 124-125 -2.8183755623213784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54.0 3 33.0 4 62.0 5 200.0 6 655.0 7 1196.0 8 1946.0 9 4075.0 10 9273.0 11 11848.0 12 9102.0 13 10643.0 14 14519.0 15 14197.0 16 9153.0 17 8497.0 18 8672.0 19 8713.0 20 9014.0 21 8494.0 22 8892.0 23 9399.0 24 10765.0 25 11959.0 26 14328.0 27 17111.0 28 21503.0 29 26406.0 30 33034.0 31 40745.0 32 52693.0 33 69280.0 34 97399.0 35 151416.0 36 243845.0 37 345669.0 38 132908.0 39 803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.67434885656735 16.732573515966262 10.562816958287707 23.03026066917868 2 12.50880333197744 22.7038196062452 36.40219189748022 28.385185164297138 3 22.646889837468585 30.163447879618893 28.098019379694676 19.09164290321785 4 13.417967276723807 18.90643714738305 33.737163385855915 33.93843219003723 5 11.258857061080677 39.03543247413995 33.950064187476784 15.75564627730259 6 30.79795127763149 28.06798994673745 21.29585567293762 19.83820310269344 7 23.211968126917075 31.689367860861573 24.281336424859763 20.81732758736159 8 28.336885204874722 31.827682884960957 23.335690281501385 16.499741628662935 9 29.81259794670571 14.589838146042899 22.134351685335435 33.46321222191595 10-11 23.81908789630744 27.21563114865622 26.640869481234063 22.324411473802275 12-13 27.703693520300614 25.090170117665306 26.07735312986894 21.12878323216514 14-15 28.17724349619804 22.169113655622436 26.394417114682074 23.259225733497452 16-17 22.653207326500034 25.603906113136144 33.21824652827346 18.52464003209036 18-19 25.398008179056625 23.95331409706444 32.21249050934754 18.436187214531397 20-21 20.88032552742408 24.86253064861565 34.25831407587462 19.99882974808565 22-23 20.04408174544819 27.072487083195572 27.28210977644711 25.60132139490913 24-25 20.696178571604815 29.001600985829405 26.558423293321614 23.743797149244166 26-27 23.476860432245026 23.049860380782246 27.63935309174967 25.833926095223052 28-29 22.31402132963364 23.28801068173615 31.04538186924743 23.35258611938278 30-31 25.67950251709375 24.58123046793763 30.133323839743504 19.605943175225114 32-33 19.061177961806163 24.847391718440807 32.22521520957687 23.866215110176164 34-35 17.1614381388791 26.795805428269297 33.172893902009164 22.86986253084244 36-37 23.859658893437512 24.528040516009504 29.171392899969756 22.440907690583227 38-39 25.816090365815747 23.61365977182956 28.764907462173095 21.8053424001816 40-41 21.993964753625395 26.156776116751455 29.208625587372016 22.64063354225113 42-43 25.170479259443596 25.100370038512487 27.956025409922162 21.773125292121755 44-45 27.074919228114748 22.1604355583817 28.255707962137492 22.508937251366053 46-47 22.846688158838095 24.237064337635292 34.25718416835801 18.659063335168604 48-49 25.658776412565093 24.041541035219574 31.1318426987362 19.16783985347913 50-51 20.753739334692046 25.562336579248097 32.62447470957017 21.059449376489688 52-53 20.25430366281025 27.832091764475315 25.675272699843006 26.238331872871434 54-55 21.07266755539827 29.133113053850508 25.039531167055927 24.754688223695297 56-57 23.714294173920216 23.67576758846134 25.932375091734162 26.67756314588428 58-59 22.911968338407938 23.830578899838635 29.13815358607431 24.119299175679114 60-61 25.85084536422604 25.202484876640906 28.252077368997274 20.694592390135785 62-63 19.5837366346587 25.52983748337153 30.281191201134156 24.605234680835615 64-65 17.538584745446073 26.981440266873268 31.651440499513217 23.82853448816744 66-67 23.84718093254781 25.07967918246092 27.674989302087205 23.398150582904066 68-69 25.555392629261455 24.1416114616768 27.57629356623647 22.726702342825277 70-71 22.002064150818367 26.39561057764499 27.934841075191347 23.6674841963453 72-73 24.9708318852084 25.49480049714452 26.77819754797494 22.75617006967214 74-75 26.74784860920084 22.56699149313254 27.2467203054492 23.43843959221742 76-77 22.740625491275647 24.475872769916975 33.20526386657465 19.57823787223273 78-79 25.168893077974563 24.400229538082808 30.37417668369638 20.056700700246246 80-81 20.556418359944757 25.813904960236194 31.7428750490835 21.886801630735544 82-83 20.0140147944908 27.866353284206358 25.368892936980657 26.75073898432218 84-85 20.813027273156663 28.925993002472328 24.784085453587977 25.476894270783028 86-87 23.221520464208346 23.74961314796394 25.718134847983894 27.310731539843818 88-89 22.3941329614854 23.808583850134752 28.671639991794155 25.12564319658569 90-91 25.127405620440168 25.16159664321703 27.78045274553913 21.93054499080367 92-93 19.369249651568804 25.513341196093624 29.44393412482614 25.67347502751144 94-95 17.49209107556085 26.901693170742885 30.83181617104074 24.77439958265553 96-97 23.426311644895513 24.955991221508857 27.220090421560737 24.397606712034893 98-99 24.84610859095288 24.32910113599231 27.02813596376048 23.796654309294325 100-101 21.635057007361997 26.136181786265922 27.4821096354532 24.74665157091888 102-103 24.241794018387715 25.223007979854025 26.67871975477406 23.856478246984203 104-105 25.846629857005006 22.54779221356138 27.046357821359734 24.55922010807388 106-107 22.18221687146249 24.4252467332848 32.45252620687346 20.940010188379254 108-109 24.519730948502072 24.50721544955496 29.448969022553634 21.524084579389335 110-111 20.025488305521232 25.901441693297773 31.13835425924392 22.934715741937076 112-113 19.551823394515626 27.546239254319865 25.24119502010326 27.66074233106125 114-115 20.175833599860077 28.719264647128583 24.729156521312913 26.375745231698428 116-117 22.505768065072026 24.01521506781157 25.427795955601677 28.051220911514722 118-119 21.616395092162104 24.033189005207728 28.09028893007625 26.260126972553916 120-121 23.995318371865107 25.232142479429744 27.35163680718602 23.42090234151913 122-123 18.91503767455501 25.711121325602903 28.668087452342817 26.705753547499267 124-125 17.318568676626878 27.151505751765537 29.742100244804732 25.787825326802853 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 687.0 1 634.5 2 2629.5 3 3544.0 4 1606.0 5 807.5 6 1030.0 7 2046.5 8 3088.0 9 3136.0 10 2569.5 11 1938.0 12 1734.0 13 1882.5 14 2157.0 15 2550.0 16 2778.0 17 2966.5 18 3269.5 19 3374.0 20 3500.5 21 3823.5 22 4245.5 23 4733.5 24 5245.0 25 5810.5 26 6608.5 27 7578.5 28 8320.5 29 9202.0 30 10561.5 31 12110.0 32 13672.5 33 14976.5 34 16287.5 35 17298.5 36 18775.5 37 20350.5 38 20619.0 39 20503.5 40 20456.5 41 21539.0 42 22667.0 43 23672.5 44 25512.0 45 27366.0 46 30937.5 47 41261.5 48 53817.0 49 92547.0 50 235189.0 51 243293.5 52 91521.0 53 34292.5 54 26377.5 55 23263.0 56 20411.0 57 18057.5 58 16861.5 59 15615.5 60 13435.5 61 11030.5 62 9234.5 63 7353.5 64 5722.5 65 4565.5 66 3294.5 67 2453.0 68 1877.5 69 1321.0 70 1050.5 71 888.5 72 817.5 73 792.0 74 582.0 75 427.5 76 289.5 77 158.5 78 103.0 79 63.0 80 37.0 81 15.5 82 10.5 83 4.5 84 3.0 85 1.5 86 0.5 87 0.5 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14860757940953162 2 2.8198781671637875E-4 3 4.2298172507456816E-4 4 0.0 5 0.0 6 0.003947829434029303 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005005283746715723 14-15 0.001621429946119178 16-17 4.582302021641155E-4 18-19 0.0 20-21 2.1149086253728408E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.5248477089547346E-4 30-31 0.0 32-33 0.0 34-35 7.049695417909469E-5 36-37 0.0 38-39 0.0 40-41 3.5248477089547344E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 5.992241105223048E-4 96-97 0.0023968964420892193 98-99 0.0034895992318651876 100-101 0.0 102-103 0.0038068355256711136 104-105 0.021466322547534333 106-107 0.01540358448813219 108-109 0.018293959609475073 110-111 0.014346130175445771 112-113 0.013993645404550297 114-115 0.04014801540499442 116-117 0.039795530634098956 118-119 0.022594273814399848 120-121 0.013464918248207086 122-123 0.0026083873046265035 124-125 0.0016566784232087252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1418501.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.183052426325915 #Duplication Level Percentage of deduplicated Percentage of total 1 88.51621099956735 45.30529868170582 2 8.056178455393855 8.24679608476522 3 1.6588038136940364 2.547079276838737 4 0.6333314159310287 1.2966334025934827 5 0.30324737861242035 0.7760563238832707 6 0.18020246513460206 0.5533987332202502 7 0.11658322515576605 0.4176959727624412 8 0.08005686655143544 0.32780438382315985 9 0.05852157678776519 0.2695781639519499 >10 0.33608790360901875 3.099077361887253 >50 0.027732301113333656 0.9728839874606435 >100 0.022939359670171078 2.4424905009169766 >500 0.004369400553194723 1.5387079794581824 >1k 0.002594331578459367 3.2652729352884227 >5k 0.0016385252074480213 6.085503747981137 >10k+ 0.0015019814401606862 22.85572246346303 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 74305 5.238276180277631 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 60846 4.289457673981196 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 36948 2.6047214630091906 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 36471 2.5710944158657623 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 21271 1.4995407123435232 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 21188 1.4936894651466583 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 18410 1.2978489264371333 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17436 1.229184893066695 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16660 1.1744792566237177 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 12380 0.8727522927371923 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11122 0.7840671243798912 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9515 0.6707785190140859 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8786 0.619386239417526 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8641 0.6091641810615572 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8373 0.5902709973415599 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7938 0.5596048222736537 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 7164 0.5050401797390344 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6569 0.46309449200247305 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6475 0.45646777830963814 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5988 0.42213576162441907 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5946 0.4191748895488971 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5940 0.4187519078238225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5741 0.40472301394218263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4995 0.352132286124578 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4732 0.3335915871754761 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4642 0.3272468612993576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4125 0.29079993598876563 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3938 0.27761700555727487 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 3035 0.2139582559335524 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 2767 0.195065072213555 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2317 0.1633414428329624 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2295 0.1617905098410223 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2090 0.1473386342343079 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 1918 0.1352131581155036 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1597 0.11258363582401423 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.049695417909469E-5 0.0 10-11 0.0 0.0 0.0 3.877332479850208E-4 0.0 12-13 0.0 0.0 0.0 8.812119272386836E-4 0.0 14-15 0.0 0.0 0.0 0.002150157102462388 0.0 16-17 0.0 0.0 0.0 0.0037010900944024717 0.0 18-19 0.0 0.0 0.0 0.005393016994700744 0.0 20-21 0.0 0.0 0.0 0.007225937803357206 0.0 22-23 0.0 0.0 0.0 0.01113851876029696 0.0 24-25 0.0 0.0 0.0 0.01487485733178898 0.0 26-27 0.0 0.0 0.0 0.019280916967982398 0.0 28-29 0.0 0.0 0.0 0.025555145889921825 0.0 30-31 0.0 0.0 0.0 0.03232285349111492 0.0 32-33 0.0 0.0 0.0 0.04243916641581501 0.0 34-35 0.0 0.0 0.0 0.05812473872066357 0.0 36-37 0.0 0.0 0.0 0.07934432192857108 0.0 38-39 0.0 0.0 0.0 0.11730693175401358 0.0 40-41 0.0 0.0 0.0 0.1869226740058696 0.0 42-43 0.0 0.0 0.0 0.23479010589347488 0.0 44-45 0.0 0.0 0.0 0.2872045913256318 0.0 46-47 0.0 0.0 0.0 0.35431769170412997 0.0 48-49 0.0 0.0 0.0 0.4129006606269576 0.0 50-51 0.0 0.0 0.0 0.47906205212403796 0.0 52-53 0.0 0.0 0.0 0.5653150755621603 0.0 54-55 0.0 0.0 0.0 0.6550929467092375 0.0 56-57 0.0 0.0 0.0 0.7608383779778796 0.0 58-59 0.0 0.0 0.0 0.8834678297724147 0.0 60-61 0.0 0.0 0.0 0.9941127993565038 0.0 62-63 0.0 0.0 0.0 1.0998582306251459 0.0 64-65 0.0 0.0 0.0 1.230594832150277 0.0 66-67 0.0 0.0 0.0 1.3820222897269723 0.0 68-69 0.0 0.0 0.0 1.5649266373446333 0.0 70-71 0.0 0.0 0.0 1.8525189619182503 0.0 72-73 1.0574543126864204E-4 0.0 0.0 2.066864951099788 0.0 74-75 2.1149086253728408E-4 0.0 0.0 2.2828323702274442 0.0 76-77 2.1149086253728408E-4 0.0 0.0 2.5339425210133797 0.0 78-79 2.1149086253728408E-4 0.0 0.0 2.771235268780212 0.0 80-81 3.5248477089547346E-4 0.0 0.0 3.01127739776003 0.0 82-83 3.5248477089547346E-4 0.0 0.0 3.3230149291399864 0.0 84-85 3.5248477089547346E-4 0.0 0.0 3.6230499661262137 0.0 86-87 3.5248477089547346E-4 0.0 0.0 3.935421970093782 0.0 88-89 3.5248477089547346E-4 0.0 0.0 4.323965933051863 0.0 90-91 3.5248477089547346E-4 0.0 0.0 4.657064041548085 0.0 92-93 3.5248477089547346E-4 0.0 0.0 4.988399726189829 0.0 94-95 3.5248477089547346E-4 0.0 0.0 5.372890114282613 0.0 96-97 3.5248477089547346E-4 0.0 0.0 5.766968088143752 0.0 98-99 3.5248477089547346E-4 0.0 0.0 6.22579751441839 0.0 100-101 3.877332479850208E-4 0.0 0.0 6.930696559255157 0.0 102-103 4.2298172507456816E-4 0.0 0.0 7.478246402364186 0.0 104-105 4.2298172507456816E-4 0.0 0.0 8.010110673168366 0.0 106-107 4.2298172507456816E-4 0.0 0.0 8.55938769165478 0.0 108-109 4.2298172507456816E-4 0.0 0.0 9.04010642220203 0.0 110-111 4.2298172507456816E-4 0.0 0.0 9.538942869973303 0.0 112-113 4.2298172507456816E-4 0.0 0.0 10.175001639054184 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 75 3.237801E-10 63.46172 2 AGTACTC 1895 0.0 59.964188 5 AAAGTAC 1990 0.0 55.608734 3 AAGTACT 2075 0.0 55.335907 4 ATAACGC 200 0.0 53.545826 3 GTATACG 35 0.007745909 51.084263 1 AAAAGTA 2300 0.0 47.59629 2 TATAACG 225 0.0 47.59629 2 AAAAAGT 2365 0.0 46.11624 1 ACCGTAT 70 5.456263E-5 42.49519 8 TAACGCA 290 0.0 41.02984 4 GTATAAC 340 0.0 36.81072 1 GTTATCA 595 0.0 36.059483 1 TCACGCA 155 5.127731E-9 34.544476 4 ATCACGC 140 8.0735845E-8 33.99735 3 TACCGTA 70 0.0027907537 33.99615 7 TACAACG 235 1.0913936E-11 30.380613 2 CCGTATG 105 5.9102493E-4 28.330128 9 GTACCGT 90 0.009533319 26.442385 6 TTATCAA 875 0.0 25.15804 2 >>END_MODULE