##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1715476 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01607775334659 33.0 31.0 34.0 30.0 34.0 2 31.807056467126326 33.0 31.0 34.0 30.0 34.0 3 32.058231068228295 34.0 31.0 34.0 30.0 34.0 4 35.53557379992492 37.0 35.0 37.0 33.0 37.0 5 35.6738928437355 37.0 35.0 37.0 33.0 37.0 6 35.81366862608395 37.0 35.0 37.0 33.0 37.0 7 35.778008552728224 37.0 35.0 37.0 33.0 37.0 8 35.82645574755928 37.0 35.0 37.0 33.0 37.0 9 37.47016163443849 39.0 37.0 39.0 34.0 39.0 10-11 37.42844609892531 39.0 37.0 39.0 34.0 39.0 12-13 37.41638501500458 39.0 37.0 39.0 34.0 39.0 14-15 38.68415559296662 40.0 38.0 41.0 34.0 41.0 16-17 38.65493309145683 40.0 38.0 41.0 34.0 41.0 18-19 38.736348395430774 40.0 38.0 41.0 34.0 41.0 20-21 38.79382486260373 40.0 38.0 41.0 34.0 41.0 22-23 38.77031797588541 40.0 38.0 41.0 34.0 41.0 24-25 38.74691776509843 40.0 38.0 41.0 34.0 41.0 26-27 38.56040306014191 40.0 38.0 41.0 34.0 41.0 28-29 38.404613646591386 40.0 38.0 41.0 34.0 41.0 30-31 38.27147742084413 40.0 38.0 41.0 34.0 41.0 32-33 37.9863253697516 40.0 37.0 41.0 33.5 41.0 34-35 37.780892300446055 40.0 37.0 41.0 33.0 41.0 36-37 37.678266848384936 40.0 37.0 41.0 33.0 41.0 38-39 37.57078385241181 40.0 37.0 41.0 33.0 41.0 40-41 37.32508353366646 40.0 37.0 41.0 32.0 41.0 42-43 37.14402095978026 40.0 36.0 41.0 31.5 41.0 44-45 36.906472605854006 40.0 36.0 41.0 31.0 41.0 46-47 36.551186959188 40.0 36.0 41.0 30.0 41.0 48-49 36.1949456011043 39.5 35.0 41.0 30.0 41.0 50-51 36.0318684143643 39.0 35.0 41.0 28.5 41.0 52-53 35.92239442580368 39.0 35.0 41.0 28.0 41.0 54-55 35.729472752752 39.0 35.0 41.0 28.0 41.0 56-57 35.44494297792566 39.0 35.0 41.0 27.0 41.0 58-59 35.274805943073524 39.0 35.0 41.0 27.0 41.0 60-61 35.11612287201919 38.5 35.0 40.0 27.0 41.0 62-63 34.776056324891755 38.0 34.0 40.0 26.0 41.0 64-65 34.502505427065145 37.5 34.0 40.0 26.0 41.0 66-67 34.06348500357918 37.0 34.0 40.0 26.0 41.0 68-69 33.734306979520554 36.5 34.0 39.0 25.5 41.0 70-71 33.274961585006146 36.0 33.0 39.0 24.5 40.5 72-73 32.954840522397284 35.0 33.0 38.5 24.5 40.0 74-75 32.61356002648827 35.0 33.0 37.0 23.5 39.0 76-77 32.15194529098629 35.0 33.0 37.0 22.5 39.0 78-79 31.75808784267457 35.0 32.5 36.0 21.0 39.0 80-81 31.518073117898474 35.0 32.0 36.0 20.5 37.0 82-83 31.290584945519495 35.0 32.0 36.0 20.0 37.0 84-85 31.029282834618495 35.0 32.0 35.0 20.0 36.5 86-87 30.74252743844857 35.0 32.0 35.0 18.0 36.0 88-89 30.525857546243728 35.0 32.0 35.0 16.0 36.0 90-91 30.352064674760825 35.0 31.0 35.0 12.0 36.0 92-93 30.1503891631244 35.0 31.0 35.0 10.0 35.0 94-95 30.07242013295435 34.5 31.0 35.0 7.0 35.0 96-97 29.974073085254474 35.0 31.0 35.0 6.0 35.0 98-99 29.85363304412303 35.0 31.0 35.0 2.0 35.0 100-101 29.60518246830617 34.0 31.0 35.0 2.0 35.0 102-103 28.2251407189608 33.0 28.0 34.5 2.0 35.0 104-105 29.10047357118374 34.0 29.5 35.0 2.0 35.0 106-107 29.189275163278296 34.0 30.0 35.0 2.0 35.0 108-109 29.141867621581415 34.0 30.0 35.0 2.0 35.0 110-111 29.145368107743856 34.0 30.0 35.0 2.0 35.0 112-113 29.067917592551574 34.0 30.0 35.0 2.0 35.0 114-115 28.943998925079686 34.0 30.0 35.0 2.0 35.0 116-117 28.70364639318766 34.0 30.0 35.0 2.0 35.0 118-119 28.50497908452231 34.0 29.0 35.0 2.0 35.0 120-121 28.31327340050225 34.0 29.0 35.0 2.0 35.0 122-123 28.003346010086993 34.0 29.0 35.0 2.0 35.0 124-125 26.952829418773565 33.0 26.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3686157769155898 1101 2 0.6390969326252147 1101 3 0.4870456587728995 1101 4 0.5350840017959797 1101 5 0.20273118868323792 1101 6 0.2645502738439447 1101 7 0.25238819447420724 1101 8 0.1939893549227989 1101 9 0.2889059757905841 1101 10-11 0.32892110120022267 1101 12-13 0.30132426328385264 1101 14-15 0.3989639317081526 1101 16-17 0.47019153270547065 1101 18-19 0.3451347784210057 1101 20-21 0.253491358562556 1101 22-23 0.24625862301885348 1101 24-25 0.3025535927923215 1101 26-27 0.2875562600518151 1101 28-29 0.2949820347575809 1101 30-31 0.26133090000783454 1101 32-33 0.2763607746401675 1101 34-35 0.2819144757718348 1101 36-37 0.21009861139751962 1101 38-39 0.14596181571292988 1101 40-41 0.23017645040347645 1101 42-43 0.0967691767879657 1101 44-45 0.07344036522484743 1101 46-47 0.13730181816697495 1101 48-49 0.14760530013287365 1101 50-51 0.1897068499313903 1101 52-53 0.18917354559136612 1101 54-55 0.22520757656745616 1101 56-57 0.31810290262351515 1101 58-59 0.17748065992620354 1101 60-61 0.040724293131155775 1101 62-63 0.13454350841087148 1101 64-65 0.24598770457728136 1101 66-67 0.24363479272352606 1101 68-69 0.25274912847513065 1101 70-71 0.2021887314370474 1101 72-73 0.20113518756306803 1101 74-75 0.10274438596385949 1101 76-77 0.11599001831260836 1101 78-79 0.13637992777465868 1101 80-81 0.11960381366688466 1101 82-83 0.10968352771864787 1101 84-85 0.1598681281566563 1101 86-87 0.18416367580034176 1101 88-89 0.126883583826217 1101 90-91 0.18964938059065872 1101 92-93 0.18650945247554063 1101 94-95 0.11607639181152152 1101 96-97 0.1716217341752575 1101 98-99 0.06142466571581906 1101 100-101 0.1664588768569466 1101 102-103 0.1180798069746416 1101 104-105 0.13446231827336774 1101 106-107 0.11004186051787457 1101 108-109 0.08814250052189365 1101 110-111 0.18187323824801638 1101 112-113 0.056317703350497794 1101 114-115 0.10835473598490708 1101 116-117 0.1129976332592193 1101 118-119 0.0752846315023632 1101 120-121 -0.05977945788667327 1101 122-123 -0.11735005354663741 1101 124-125 0.006622969247771948 1102 1 0.3462472379193642 1102 2 0.6097285484567578 1102 3 0.44928397946195986 1102 4 0.3928105189044473 1102 5 0.27749670609321697 1102 6 0.2183311273524282 1102 7 0.17854247627705178 1102 8 0.1851101857775319 1102 9 0.28973715647459386 1102 10-11 0.24007898886044643 1102 12-13 0.15573879020351455 1102 14-15 0.3346992006602889 1102 16-17 0.36908135644627293 1102 18-19 0.27064224682391824 1102 20-21 0.23528279740150992 1102 22-23 0.19656960275271018 1102 24-25 0.22142231106282395 1102 26-27 0.2547786090336217 1102 28-29 0.38234615301340824 1102 30-31 0.325469324187047 1102 32-33 0.2914967175005785 1102 34-35 0.18699537328399174 1102 36-37 0.31353018567003943 1102 38-39 0.2716697453396293 1102 40-41 0.33323470639082586 1102 42-43 0.33782736351025733 1102 44-45 0.3982374593519822 1102 46-47 0.40394987833793294 1102 48-49 0.270350111365218 1102 50-51 0.3260215007132743 1102 52-53 0.28710109434173603 1102 54-55 0.39039760728853423 1102 56-57 0.4192706299962552 1102 58-59 0.43823762513885356 1102 60-61 0.44093728318143377 1102 62-63 0.4784297466663716 1102 64-65 0.3939134198408567 1102 66-67 0.4160477428445333 1102 68-69 0.4236357008326621 1102 70-71 0.3786898478267844 1102 72-73 0.30916677650347424 1102 74-75 0.3168844399091171 1102 76-77 0.33425083384753407 1102 78-79 0.2579533446606668 1102 80-81 0.3109042243679241 1102 82-83 0.3453721446176914 1102 84-85 0.4952128644197664 1102 86-87 0.37452697102431287 1102 88-89 0.36562801206354223 1102 90-91 0.3316203381596985 1102 92-93 0.30435427022695194 1102 94-95 0.3031282061485747 1102 96-97 0.3475977603167024 1102 98-99 0.28194830954674543 1102 100-101 0.2243960412269992 1102 102-103 0.214802856755842 1102 104-105 0.34067610690900807 1102 106-107 0.42489076083323596 1102 108-109 0.3421583459222859 1102 110-111 0.47046507963918316 1102 112-113 0.5073860149943599 1102 114-115 0.613932100979163 1102 116-117 0.4483464439374494 1102 118-119 0.47327655601160146 1102 120-121 0.6453067363651392 1102 122-123 0.5902729734178429 1102 124-125 0.47152952253704683 1103 1 0.29859811544125847 1103 2 0.6219522210423136 1103 3 0.423136428297596 1103 4 0.5671522310153989 1103 5 0.27665070421372917 1103 6 0.24270553122575222 1103 7 0.28681109007602856 1103 8 0.22070466028381475 1103 9 0.34010121850223385 1103 10-11 0.3450416628356976 1103 12-13 0.2808112920958905 1103 14-15 0.3852135393375775 1103 16-17 0.42031588058779334 1103 18-19 0.27237701937595915 1103 20-21 0.19364122566594233 1103 22-23 0.3585284310518304 1103 24-25 0.3084672042345744 1103 26-27 0.25300956285973086 1103 28-29 0.3265104683729092 1103 30-31 0.35560872701752544 1103 32-33 0.24034831681908742 1103 34-35 0.31471104517587634 1103 36-37 0.2992247567283286 1103 38-39 0.3116339601631779 1103 40-41 0.37281810319382913 1103 42-43 0.37320550408718134 1103 44-45 0.41950966104257503 1103 46-47 0.38976352826188787 1103 48-49 0.3596405126419526 1103 50-51 0.5051369131802019 1103 52-53 0.3833639806376823 1103 54-55 0.4545323418759608 1103 56-57 0.5030048963532678 1103 58-59 0.40024180007610255 1103 60-61 0.415607172545009 1103 62-63 0.40543703845265355 1103 64-65 0.28152230450103843 1103 66-67 0.32633580531275186 1103 68-69 0.38277173020135535 1103 70-71 0.36508541697983077 1103 72-73 0.3711655614010496 1103 74-75 0.3090082444334641 1103 76-77 0.3204937828554151 1103 78-79 0.2570396743483023 1103 80-81 0.2880778457481057 1103 82-83 0.3067197014064469 1103 84-85 0.233937135768425 1103 86-87 0.30434733623465604 1103 88-89 0.278205669646038 1103 90-91 0.24426537123813574 1103 92-93 0.1826663617263904 1103 94-95 0.250416693961224 1103 96-97 0.20700794936648848 1103 98-99 0.29232179242633904 1103 100-101 0.3169831548138866 1103 102-103 0.17556087795510322 1103 104-105 0.31732332061001856 1103 106-107 0.31401808411609977 1103 108-109 0.3323565294789148 1103 110-111 0.363909561058783 1103 112-113 0.42326155663475973 1103 114-115 0.43282046974224286 1103 116-117 0.5087764050588568 1103 118-119 0.4726622672507723 1103 120-121 0.5800876956984631 1103 122-123 0.6093657242831796 1103 124-125 0.7269706797082662 1104 1 0.26581555002740487 1104 2 0.6032794835089419 1104 3 0.08470981691247914 1104 4 0.4210751104938453 1104 5 0.180742626060983 1104 6 0.1231174561042323 1104 7 0.13835520557022818 1104 8 0.12015416027549009 1104 9 0.21091914933637668 1104 10-11 0.21516666061107514 1104 12-13 0.23571825707779226 1104 14-15 0.34688865764426424 1104 16-17 0.43989956388006135 1104 18-19 0.308616930368224 1104 20-21 0.24314015526520194 1104 22-23 0.20325588216616808 1104 24-25 0.2583594465418457 1104 26-27 0.2408447699445162 1104 28-29 0.36255777038321924 1104 30-31 0.2963435747760528 1104 32-33 0.26883205643011365 1104 34-35 0.27698537547932034 1104 36-37 0.2594177044265322 1104 38-39 0.2883446350639005 1104 40-41 0.4118716553799544 1104 42-43 0.4538125065575116 1104 44-45 0.39019649720898286 1104 46-47 0.3744809671537439 1104 48-49 0.4364899176782657 1104 50-51 0.5383135950355182 1104 52-53 0.5084817419414733 1104 54-55 0.479223320714226 1104 56-57 0.5012928005666097 1104 58-59 0.503765539778982 1104 60-61 0.4368531488543894 1104 62-63 0.43601220733714285 1104 64-65 0.3255011640147387 1104 66-67 0.38374074517339807 1104 68-69 0.45508592568425854 1104 70-71 0.4009853200151241 1104 72-73 0.42589736989891946 1104 74-75 0.4295668277039866 1104 76-77 0.3869219224822871 1104 78-79 0.3167081285564457 1104 80-81 0.32849473081020264 1104 82-83 0.30489206893538423 1104 84-85 0.333050962222849 1104 86-87 0.3697505721803722 1104 88-89 0.35858145522373164 1104 90-91 0.35067953738477087 1104 92-93 0.17798317910265027 1104 94-95 0.18359630370270352 1104 96-97 0.2858767347128186 1104 98-99 0.2860526714638816 1104 100-101 0.2657975331461522 1104 102-103 0.15336204692800592 1104 104-105 0.30332027446021925 1104 106-107 0.27718140103221955 1104 108-109 0.29640452729276134 1104 110-111 0.3019585038335322 1104 112-113 0.30271675165207057 1104 114-115 0.34255804502766907 1104 116-117 0.3268021084548032 1104 118-119 0.39136104000719385 1104 120-121 0.3758748119659714 1104 122-123 0.2797404248207016 1104 124-125 0.2979670393698015 1105 1 0.13810809573767102 1105 2 0.5162780599219019 1105 3 0.1880197281023328 1105 4 0.39449768190855394 1105 5 0.15938539700611898 1105 6 0.17990602632644226 1105 7 0.1922415411316578 1105 8 0.16679303989251792 1105 9 0.25547799426238527 1105 10-11 0.21196858231093074 1105 12-13 0.18416828746096314 1105 14-15 0.3512927908965864 1105 16-17 0.35453055813722045 1105 18-19 0.21106370276261543 1105 20-21 0.11615191198303165 1105 22-23 0.16407985092143207 1105 24-25 0.21234263737382975 1105 26-27 0.2507157341893844 1105 28-29 0.31957713858444947 1105 30-31 0.2502323352853466 1105 32-33 0.316310823871909 1105 34-35 0.2225517149206766 1105 36-37 0.21752915503034131 1105 38-39 0.158881661164898 1105 40-41 0.14839850240568353 1105 42-43 0.2123782782757715 1105 44-45 0.2506367536258196 1105 46-47 0.2862286984010538 1105 48-49 0.20827736101293937 1105 50-51 0.24765649122207378 1105 52-53 0.18098625662382517 1105 54-55 0.1255764844153262 1105 56-57 0.23085106065224892 1105 58-59 0.30313633713036836 1105 60-61 0.15496693881967616 1105 62-63 0.21171722526749903 1105 64-65 0.2721354790240298 1105 66-67 0.24049137209374294 1105 68-69 0.3372268978886481 1105 70-71 0.22267676394319835 1105 72-73 0.2498518607597191 1105 74-75 0.16623432170580799 1105 76-77 0.1070872183401903 1105 78-79 0.05031933238615238 1105 80-81 0.08190991326613073 1105 82-83 0.11634595355921462 1105 84-85 0.12679702168257023 1105 86-87 0.21076400068577428 1105 88-89 0.18709384956019548 1105 90-91 0.22069905108287458 1105 92-93 0.2852931131155394 1105 94-95 0.1678676639272787 1105 96-97 0.23604120323556899 1105 98-99 0.1602402426075713 1105 100-101 0.11023755562684556 1105 102-103 0.0664820133781383 1105 104-105 0.04125387653483514 1105 106-107 0.10785827530295222 1105 108-109 0.1206079319195581 1105 110-111 0.1722203696740081 1105 112-113 0.19314106047060875 1105 114-115 0.15607085303171075 1105 116-117 0.1259489547387851 1105 118-119 0.17817617054070567 1105 120-121 0.11061304597629373 1105 122-123 0.2517529603747164 1105 124-125 0.3847631982523829 1106 1 0.13588422314617787 1106 2 0.4083858802535971 1106 3 0.2956344750054001 1106 4 0.44309892281552266 1106 5 0.16703357811422848 1106 6 0.09617368776741841 1106 7 0.08699151730089483 1106 8 0.011871026954196395 1106 9 0.16757534328561263 1106 10-11 0.17711200082324297 1106 12-13 0.10738514171485747 1106 14-15 0.24117616954393384 1106 16-17 0.16895194009384795 1106 18-19 0.12192334596985432 1106 20-21 0.13536598052741766 1106 22-23 0.10585788194553203 1106 24-25 0.09458800744779694 1106 26-27 0.16635661099073218 1106 28-29 0.25122261365605425 1106 30-31 0.19275118292922855 1106 32-33 0.22730413276270411 1106 34-35 0.1143757001925465 1106 36-37 0.17805815529069946 1106 38-39 0.20217966805945764 1106 40-41 0.19178671051724905 1106 42-43 0.23261770986583485 1106 44-45 0.239214206451571 1106 46-47 0.19510690257165209 1106 48-49 0.23355610980539154 1106 50-51 0.2549195387427474 1106 52-53 0.2185993104756534 1106 54-55 0.21074279468759016 1106 56-57 0.29680294669710605 1106 58-59 0.23398714517108488 1106 60-61 0.16782751249372296 1106 62-63 0.12016590660822146 1106 64-65 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110-111 0.023336851931318847 2207 112-113 0.14249580677346785 2207 114-115 0.08237335598584039 2207 116-117 0.04817941271851822 2207 118-119 0.09490979749670103 2207 120-121 0.11092564668528482 2207 122-123 0.27238174255683845 2207 124-125 0.2905318092739755 2208 1 0.08634657812249458 2208 2 -0.19839202842354808 2208 3 0.17099112545556494 2208 4 0.3809511406075998 2208 5 0.15438442657200824 2208 6 0.13500439959134525 2208 7 0.0685492909465566 2208 8 0.06605263461048594 2208 9 0.11192275499646342 2208 10-11 -0.006430488947685831 2208 12-13 0.07241740867896596 2208 14-15 0.06773338235819892 2208 16-17 -0.29162544453686223 2208 18-19 0.046627237443054526 2208 20-21 0.14134186547406813 2208 22-23 0.17000201491760691 2208 24-25 0.20286580918379116 2208 26-27 0.19780155650649078 2208 28-29 0.17455654381616625 2208 30-31 0.26911015403700844 2208 32-33 0.21723884641473035 2208 34-35 0.20220997834680787 2208 36-37 0.20793695300752546 2208 38-39 0.170193328658236 2208 40-41 0.21452316365230217 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120-121 -2.184064220801819 2216 122-123 -2.3561487445413327 2216 124-125 -2.532196980057556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 20.0 4 51.0 5 224.0 6 655.0 7 1091.0 8 1985.0 9 3843.0 10 7926.0 11 10649.0 12 9936.0 13 12329.0 14 17459.0 15 17433.0 16 10299.0 17 9455.0 18 9514.0 19 9588.0 20 9556.0 21 9524.0 22 9994.0 23 10587.0 24 11871.0 25 13730.0 26 16395.0 27 19714.0 28 24588.0 29 30725.0 30 38066.0 31 48561.0 32 62205.0 33 82080.0 34 114227.0 35 171083.0 36 275503.0 37 438826.0 38 204032.0 39 1729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.23367282721774 18.353511093779005 11.728710020537765 23.684106058465492 2 13.118582316851018 23.05967734883924 36.73944534312653 27.082294991183197 3 22.011577002220967 28.307752394387542 28.986167056258633 20.694503547132857 4 13.632542804446112 18.793617631491202 33.71717237664648 33.85666718741621 5 11.62015673783836 39.155662918047234 34.98824815969445 14.23593218441995 6 32.076639806086156 30.61278383305128 19.241973159628564 18.068603201233994 7 23.537723640552244 33.12981353280372 23.74308938160604 19.589373445037992 8 27.81525360891088 32.70392590744493 22.717601412086207 16.76321907155798 9 28.770323805171277 14.436692789639727 21.039291718450155 35.75369168673884 10-11 23.784702133433054 27.443252581137173 26.440264778054118 22.331780507375658 12-13 27.140112667960636 25.18164189053888 26.724931203871762 20.95331423762872 14-15 27.20889820035349 23.38205529984004 25.992481264369427 23.41656523543704 16-17 22.080187564250053 27.231384134510428 31.3232472468843 19.36518105435522 18-19 23.848949212929824 25.326265129911462 32.214994555447 18.60979110171171 20-21 21.13485835401761 25.635975829420904 32.65246223492749 20.576703581634 22-23 19.97667119796488 26.897549135050564 28.452132236184006 24.673647430800546 24-25 21.171762239751533 28.033706096733503 27.306007195670475 23.488524467844492 26-27 23.02757368800263 24.320217828754235 28.595649254201167 24.05655922904197 28-29 22.240590997868516 23.70616474829223 31.547915543918975 22.505328709920274 30-31 24.153267081556372 25.495139541445056 30.073664685486712 20.27792869151186 32-33 19.556904322765227 25.75865240901068 31.72956077496858 22.954882493255514 34-35 18.157507395910756 27.10144420641513 32.66512190501173 22.075926492662383 36-37 23.46611086369031 25.333639176531765 29.640198988502313 21.56005097127561 38-39 24.73415541808804 24.048952010987037 29.381611867493334 21.835280703431582 40-41 21.528905988137396 26.342674769378743 29.565076728019935 22.563342514463926 42-43 24.30590693195358 25.357626687869722 28.99046678589499 21.345999594281704 44-45 25.91003896294673 23.440287127304607 28.59375473629477 22.055919173453898 46-47 22.26067283949178 25.425975064646778 32.90993286994397 19.40341922591747 48-49 24.284047109956653 25.23952535622766 31.087581522562836 19.388846011252852 50-51 20.91745964385395 26.212841217248155 31.164732120997318 21.70496701790057 52-53 20.09284886527121 27.46447633193353 26.986562330222046 25.45611247257321 54-55 21.29802457160578 28.35822244088521 25.765968162772314 24.57778482473669 56-57 23.24494775794007 24.905594715402604 26.92278994284968 24.926667583807642 58-59 22.708536872564817 24.156764653075882 29.666984557055887 23.467713917303417 60-61 24.341698747169882 25.97885368259305 28.371134309078062 21.308313261159 62-63 19.89465314583241 26.235371407119658 29.996368354905577 23.873607092142358 64-65 18.4412955937594 27.26613488034808 31.147739752698378 23.144829773194147 66-67 23.35127393213312 25.907153466443134 28.133911520767413 22.60766108065633 68-69 24.590521814353565 24.583672403461197 27.965211987809795 22.86059379437544 70-71 21.616973947755607 26.607367284648692 28.15262352839678 23.623035239198916 72-73 24.116775752036173 25.80884256031562 27.70003777377241 22.374343913875798 74-75 25.53996674975342 23.849969337956345 27.55162415562794 23.058439756662292 76-77 22.018961501064428 25.63999729521136 31.906304722421062 20.434736481303148 78-79 23.702138648398464 25.395575338856386 30.43939408070996 20.46289193203519 80-81 20.48378992186425 26.41252340458275 30.564636287537688 22.53905038601531 82-83 19.77611461775041 27.319239674585944 26.7481445383089 26.156501169354744 84-85 20.910377061526948 28.00855272822237 25.643115963149587 25.437954247101096 86-87 22.65954172486237 24.589414250039056 26.916319435538593 25.834724589559983 88-89 22.22065479202274 23.94778475478526 29.287264875754598 24.5442955774374 90-91 23.56069102686368 25.799952899370204 27.98409887401514 22.655257199750974 92-93 19.601265188204323 26.004356808256134 29.33419062697467 25.060187376564873 94-95 18.2262032694992 26.934880205705582 30.50730080506033 24.331615719734895 96-97 22.78155671036704 25.55310427726202 27.844772583457868 23.820566428913075 98-99 23.78444866111116 24.4985324250983 27.497281998816614 24.21973691497393 100-101 21.08726674112608 26.021961251570993 27.957225866173584 24.93354614112934 102-103 23.284506541410472 25.205146852005416 27.65286001804225 23.857486588541857 104-105 24.54119380420324 23.336853959509206 27.622096394414303 24.49985584187326 106-107 21.332575409752437 25.170933630672582 31.4834167778045 22.01307418177048 108-109 22.823643617060043 25.208790343856542 29.766015470702012 22.201550568381407 110-111 19.876314144000887 26.003034574030053 30.132562203666268 23.988089078302796 112-113 19.17639706070973 26.741734703634002 26.723574022121632 27.358294213534634 114-115 19.968772141601278 27.57848514271219 25.731988581858683 26.720754133827846 116-117 21.606720487795776 24.574968976263342 26.704351953795623 27.11395858214526 118-119 21.04308938704651 24.138511075900933 28.85891326877918 25.95948626827338 120-121 22.28787173441127 25.63131338895388 27.70453463584246 24.376280240792397 122-123 18.96903802959846 25.93473811476676 28.624884649711124 26.471339205923655 124-125 17.65640985283702 26.980113031895513 29.59555683802162 25.76792027724585 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 883.0 1 748.5 2 1645.5 3 2226.5 4 1198.0 5 728.0 6 1262.0 7 2647.5 8 3883.5 9 4130.5 10 3378.0 11 2376.5 12 2156.0 13 2316.5 14 2659.5 15 3062.5 16 3275.5 17 3556.0 18 3897.0 19 3956.5 20 4191.0 21 4755.5 22 5340.0 23 6054.0 24 6875.5 25 7875.5 26 9263.5 27 10840.0 28 12075.0 29 13420.5 30 15538.5 31 17921.0 32 20046.0 33 21969.0 34 24355.5 35 26201.5 36 27858.0 37 29776.5 38 30442.0 39 30541.5 40 31172.5 41 33236.0 42 35250.0 43 37500.0 44 40167.5 45 41889.0 46 44864.5 47 53410.0 48 66020.5 49 104873.5 50 238936.0 51 238292.5 52 93410.0 53 44412.5 54 35433.0 55 30779.5 56 26799.0 57 23363.5 58 21325.0 59 19282.5 60 16620.0 61 13859.5 62 11516.5 63 9021.0 64 6876.0 65 5468.0 66 3957.5 67 3047.5 68 2341.0 69 1602.5 70 1271.0 71 1040.5 72 912.0 73 839.0 74 654.5 75 516.5 76 320.5 77 139.5 78 77.5 79 44.5 80 22.5 81 12.5 82 7.5 83 2.0 84 0.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1477141038405667 2 5.829285865847147E-5 3 3.4975715195082884E-4 4 0.0 5 0.0 6 0.00437196439938536 7 0.0 8 0.0 9 0.0 10-11 8.743928798770721E-5 12-13 0.0047508679806654245 14-15 0.0016322000424372012 16-17 6.120750159139504E-4 18-19 0.0 20-21 1.1658571731694294E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.623178639631216E-4 30-31 0.0 32-33 0.0 34-35 5.829285865847147E-5 36-37 0.0 38-39 0.0 40-41 5.829285865847147E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.452464505478363E-4 96-97 0.0025940322103019803 98-99 0.00355586437816676 100-101 0.0 102-103 0.0034101322315205807 104-105 0.02014018266650189 106-107 0.01536016825650723 108-109 0.017400418309553734 110-111 0.014194311083337801 112-113 0.01352394320876538 114-115 0.03853157957324964 116-117 0.03847328671459117 118-119 0.02206384700223145 120-121 0.0129701610515099 122-123 0.002856350074265102 124-125 0.0018070786184126153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1715476.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.18457141923365 #Duplication Level Percentage of deduplicated Percentage of total 1 86.34105689654612 47.64694220719566 2 9.517360764254347 10.504229496352124 3 2.100135183936686 3.476851801439985 4 0.7608768211323603 1.6795464510807285 5 0.37384696302918147 1.0315292215573737 6 0.21848405488556594 0.7234169358477767 7 0.1391148317776903 0.5373894658797438 8 0.08922524650617493 0.3939085590574991 9 0.06659074227935091 0.33073034158571607 >10 0.3432011207916345 3.328069337964018 >50 0.024255959415456996 0.9146008003860862 >100 0.01873291621571245 2.260918016046147 >500 0.0029972628335756687 1.134524445671379 >1k 0.0014986314167878343 1.897582073084422 >5k 0.0016235173681868203 6.570069521002546 >10k+ 9.990876111918895E-4 17.569691325848886 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 68507 3.993468868115905 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 61070 3.5599448782728524 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 36737 2.141504748536266 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 22073 1.2866982691684408 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 19012 1.1082638288148596 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 18343 1.069265906372342 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16760 0.9769883111159817 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12435 0.7248716974180928 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9702 0.5655573147044902 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9216 0.5372269853964731 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8378 0.48837756984067393 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8187 0.4772436338369059 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7748 0.4516530688858369 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7746 0.45153648316851996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7428 0.4329993541151261 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7007 0.40845806061990964 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6436 0.37517283832592235 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6351 0.37021794533995234 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6053 0.3528466734597278 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5724 0.33366832296109067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5356 0.3122165509747732 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4076 0.2376016918919297 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4000 0.23317143463388584 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3265 0.19032618351990935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3023 0.17621931172455926 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2368 0.13803748930326043 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2110 0.1229979317693748 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1886 0.10994033142987718 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.10708398135561208 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1658571731694294E-4 0.0 12-13 0.0 0.0 0.0 7.578071625601291E-4 0.0 14-15 0.0 0.0 0.0 0.0017196393304249084 0.0 16-17 0.0 0.0 0.0 0.0030895215088989876 0.0 18-19 2.9146429329235735E-5 0.0 0.0 0.00396391438877606 0.0 20-21 5.829285865847147E-5 0.0 0.0 0.005858432295176383 0.0 22-23 5.829285865847147E-5 0.0 0.0 0.009064539521392313 0.0 24-25 5.829285865847147E-5 0.0 0.0 0.012532964611571365 0.0 26-27 5.829285865847147E-5 0.0 0.0 0.016147121848396596 0.0 28-29 5.829285865847147E-5 0.0 0.0 0.02232616486619457 0.0 30-31 5.829285865847147E-5 0.0 0.0 0.03462595804313205 0.0 32-33 5.829285865847147E-5 0.0 0.0 0.05540736215487713 0.0 34-35 1.1658571731694293E-4 0.0 0.0 0.08778904513965803 0.0 36-37 1.748785759754144E-4 0.0 0.0 0.13354893918655814 0.0 38-39 1.748785759754144E-4 0.0 0.0 0.206356719650989 0.0 40-41 1.748785759754144E-4 0.0 0.0 0.30868983302593567 0.0 42-43 1.748785759754144E-4 0.0 0.0 0.38659823862298276 0.0 44-45 1.748785759754144E-4 0.0 0.0 0.47447472305062854 0.0 46-47 1.748785759754144E-4 0.0 0.0 0.5866010366801984 0.0 48-49 1.748785759754144E-4 0.0 0.0 0.6923151358573364 0.0 50-51 1.748785759754144E-4 0.0 0.0 0.8008564386794103 0.0 52-53 1.748785759754144E-4 0.0 0.0 0.9321902492369465 0.0 54-55 1.748785759754144E-4 0.0 0.0 1.0664678491567354 0.0 56-57 1.748785759754144E-4 0.0 0.0 1.2184956245380292 0.0 58-59 1.748785759754144E-4 0.0 0.0 1.402584472181482 0.0 60-61 1.748785759754144E-4 0.0 0.0 1.5710508337044646 0.0 62-63 1.748785759754144E-4 0.0 0.0 1.7369523094464743 0.0 64-65 1.748785759754144E-4 0.0 0.0 1.9336907074188154 0.0 66-67 1.748785759754144E-4 0.0 0.0 2.154416616729118 0.0 68-69 1.748785759754144E-4 0.0 0.0 2.4241959666005237 0.0 70-71 1.748785759754144E-4 0.0 0.0 2.806276508677475 0.0 72-73 1.748785759754144E-4 0.0 0.0 3.1101571808640864 0.0 74-75 1.748785759754144E-4 0.0 0.0 3.3916533953258456 0.0 76-77 1.748785759754144E-4 0.0 0.0 3.7381461471917996 0.0 78-79 1.748785759754144E-4 0.0 0.0 4.085979634806899 0.0 80-81 1.748785759754144E-4 0.0 0.0 4.456022701570876 0.0 82-83 1.748785759754144E-4 0.0 0.0 4.909133091923175 0.0 84-85 1.748785759754144E-4 0.0 0.0 5.343735499651409 0.0 86-87 1.748785759754144E-4 0.0 0.0 5.7847792682614045 0.0 88-89 1.748785759754144E-4 0.0 0.0 6.297319228016014 0.0 90-91 1.748785759754144E-4 0.0 0.0 6.7767196976232835 0.0 92-93 1.748785759754144E-4 0.0 0.0 7.264397753160056 0.0 94-95 1.748785759754144E-4 0.0 0.0 7.817888446122243 0.0 96-97 1.748785759754144E-4 0.0 0.0 8.36490863177334 0.0 98-99 1.748785759754144E-4 0.0 0.0 8.997444441076413 0.0 100-101 2.0402500530465015E-4 0.0 0.0 9.863209977872032 0.0 102-103 2.3317143463388588E-4 0.0 0.0 10.591637539668291 0.0 104-105 2.3317143463388588E-4 0.0 0.0 11.258595282009193 0.0 106-107 3.497571519508288E-4 0.0 0.0 11.976879886398876 0.0 108-109 3.497571519508288E-4 0.0 0.0 12.650220696762881 0.0 110-111 3.497571519508288E-4 0.0 0.0 13.368651033299212 0.0 112-113 3.497571519508288E-4 0.0 0.0 14.218764937545032 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1700 0.0 45.84957 5 AAGTACT 1940 0.0 40.17746 4 AAAGTAC 2025 0.0 38.491 3 GTTATCA 975 0.0 37.28616 1 TATCACG 95 3.287165E-4 31.315498 2 TATACGA 100 4.4397806E-4 29.749722 4 ATAACGC 145 4.0687246E-6 28.723871 3 AAAAGTA 2680 0.0 28.417648 2 AAAAAGT 2765 0.0 28.020159 1 ATCACGC 135 8.249034E-5 26.444199 3 TTATCAA 1405 0.0 25.8325 2 TATAACG 120 0.0012881906 24.791437 2 TCACGCA 135 0.0025559291 22.036833 4 ACAACGC 250 5.281836E-7 21.4198 3 CGCATGT 200 4.849863E-5 20.858854 1 GAGTACT 11970 0.0 18.316814 12-13 ATGGGCG 195 9.909726E-4 18.308056 7 TTACTCT 1165 0.0 17.87537 4 TTTACTC 1025 0.0 17.414473 3 GTATAGG 345 5.904476E-7 17.274414 1 >>END_MODULE