##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364141_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104319 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.4275264349179 31.0 31.0 34.0 26.0 34.0 2 30.506504004383366 31.0 31.0 34.0 26.0 34.0 3 30.634678487434652 31.0 31.0 34.0 26.0 34.0 4 34.02120115818942 37.0 35.0 37.0 30.0 37.0 5 34.066088839192155 37.0 35.0 37.0 30.0 37.0 6 33.9814034849279 37.0 35.0 37.0 30.0 37.0 7 33.92077436928526 37.0 35.0 37.0 29.0 37.0 8 33.83989547212186 37.0 35.0 37.0 29.0 37.0 9 35.20537665629593 39.0 35.0 39.0 28.0 39.0 10-11 35.130014033043466 39.0 35.0 39.0 27.0 39.0 12-13 35.022911687819196 39.0 35.0 39.0 27.0 39.0 14-15 36.202093171234964 40.0 36.0 41.0 27.0 41.0 16-17 36.24769723601793 40.0 36.5 41.0 27.0 41.0 18-19 36.2391357964263 40.0 36.5 41.0 27.0 41.0 20-21 36.26471129139641 40.0 37.0 41.0 27.0 41.0 22-23 36.24750453709727 40.0 36.0 41.0 27.0 41.0 24-25 36.23288911994807 40.0 36.0 41.0 27.0 41.0 26-27 36.09632950137313 40.0 36.0 41.0 27.0 41.0 28-29 36.02409235481883 40.0 36.0 41.0 27.0 41.0 30-31 36.101745267708935 40.0 36.0 41.0 27.5 41.0 32-33 35.87621981268049 40.0 36.0 41.0 27.0 41.0 34-35 35.951710030656 40.0 36.0 41.0 27.5 41.0 36-37 35.947376086990616 40.0 35.5 41.0 27.5 41.0 38-39 35.843345519381806 40.0 35.0 41.0 27.0 41.0 40-41 35.628760420829735 40.0 35.0 41.0 27.0 41.0 42-43 35.464311969810666 40.0 35.0 41.0 26.0 41.0 44-45 35.27993498134076 39.0 35.0 41.0 25.5 41.0 46-47 35.08077149899801 39.0 35.0 41.0 25.0 41.0 48-49 34.89943611211038 39.0 34.0 41.0 24.0 41.0 50-51 34.79396422310495 39.0 34.0 41.0 24.0 41.0 52-53 34.664126494129455 39.0 34.0 41.0 23.5 41.0 54-55 34.44092792014899 39.0 34.0 41.0 23.0 41.0 56-57 34.15885566779561 38.0 33.0 40.0 22.0 41.0 58-59 33.88016241834056 38.0 33.0 40.0 21.0 41.0 60-61 33.632690195735535 38.0 33.0 40.0 20.5 41.0 62-63 33.203459646564994 37.0 32.0 40.0 18.5 41.0 64-65 32.975889349476006 37.0 32.0 40.0 18.0 41.0 66-67 32.624786688710216 36.0 31.5 39.5 15.0 41.0 68-69 32.24784645293798 36.0 31.0 39.0 10.5 41.0 70-71 31.84989704507729 35.0 31.0 39.0 7.5 41.0 72-73 31.664080873669818 35.0 31.0 38.5 7.0 40.0 74-75 31.350425006854948 35.0 31.0 37.0 2.0 39.5 76-77 30.89775124398915 35.0 31.0 37.0 2.0 39.0 78-79 30.555318133800057 35.0 30.5 36.0 2.0 39.0 80-81 30.2857045438453 35.0 30.5 36.0 2.0 38.0 82-83 30.03270630546034 35.0 30.0 36.0 2.0 37.0 84-85 29.761222751873646 35.0 30.0 35.0 2.0 37.0 86-87 29.453274194644443 35.0 30.0 35.0 2.0 36.0 88-89 29.18210380650462 35.0 30.0 35.0 2.0 36.0 90-91 29.02810576723396 34.5 29.5 35.0 2.0 36.0 92-93 28.76283800127262 34.0 29.0 35.0 2.0 36.0 94-95 28.683545365507797 34.0 29.0 35.0 2.0 35.0 96-97 28.607007777813152 34.0 29.0 35.0 2.0 35.0 98-99 28.435536864895482 34.0 29.0 35.0 2.0 35.0 100-101 28.115438058583322 34.0 28.5 35.0 2.0 35.0 102-103 27.013357765623937 32.5 25.5 34.5 2.0 35.0 104-105 27.770351120718864 34.0 27.0 35.0 2.0 35.0 106-107 27.810160674308413 34.0 27.5 35.0 2.0 35.0 108-109 27.779397515300673 34.0 28.0 35.0 2.0 35.0 110-111 27.754756526933416 34.0 28.0 35.0 2.0 35.0 112-113 27.65956064646092 34.0 27.5 35.0 2.0 35.0 114-115 27.51620357940027 34.0 27.5 35.0 2.0 35.0 116-117 27.26535449235596 34.0 26.5 35.0 2.0 35.0 118-119 27.06595958122319 34.0 26.0 35.0 2.0 35.0 120-121 26.888707700686066 34.0 25.0 35.0 2.0 35.0 122-123 26.526134583207202 34.0 25.0 35.0 2.0 35.0 124-125 25.60635008285341 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5372122872266907 1101 2 0.6177606312038684 1101 3 0.6246575420849467 1101 4 0.6367345793195724 1101 5 0.5363012527999445 1101 6 0.5973434813738265 1101 7 0.5748819332121684 1101 8 0.5560835583404042 1101 9 0.6979720558416034 1101 10-11 0.6962676568534789 1101 12-13 0.7002153029977336 1101 14-15 0.6782216235969685 1101 16-17 0.7005017269335738 1101 18-19 0.6470771242625943 1101 20-21 0.709132610569398 1101 22-23 0.7381255590888642 1101 24-25 0.7511923287640201 1101 26-27 0.9300384434651647 1101 28-29 0.9519413703521096 1101 30-31 0.9018425431052322 1101 32-33 0.9149135287336847 1101 34-35 0.8512257372611174 1101 36-37 0.8111511982986315 1101 38-39 0.7910648409347232 1101 40-41 0.8708833491524288 1101 42-43 0.9887073720050168 1101 44-45 0.8341173129881341 1101 46-47 0.9146082367394328 1101 48-49 0.8491559840724534 1101 50-51 0.7910227374741297 1101 52-53 0.7839265548167731 1101 54-55 0.8859416675729648 1101 56-57 0.8915971112534464 1101 58-59 0.8709334103487905 1101 60-61 0.8684760183886482 1101 62-63 0.8570157472064537 1101 64-65 0.8007577035772186 1101 66-67 0.873791103862871 1101 68-69 0.7706135027104395 1101 70-71 0.5996622631553379 1101 72-73 0.6296003559798109 1101 74-75 0.6321033057130769 1101 76-77 0.5497706833257112 1101 78-79 0.5259056498069157 1101 80-81 0.5850328470480335 1101 82-83 0.5671507435238645 1101 84-85 0.5681711724131375 1101 86-87 0.6491850494220763 1101 88-89 0.6094239582552525 1101 90-91 0.6302889040642299 1101 92-93 0.6213688372487525 1101 94-95 0.6758605955156014 1101 96-97 0.6672725444728442 1101 98-99 0.6263509409647625 1101 100-101 0.6151875911581257 1101 102-103 0.422903012136441 1101 104-105 0.5585754992910559 1101 106-107 0.4682008889925662 1101 108-109 0.48402041194703216 1101 110-111 0.4443862720265912 1101 112-113 0.4278563418104824 1101 114-115 0.5893245583789373 1101 116-117 0.6468440433124556 1101 118-119 0.5797894611509662 1101 120-121 0.5093920183442826 1101 122-123 0.48463274417767366 1101 124-125 0.5354896225904717 1102 1 0.5874171740478609 1102 2 0.5922235714269171 1102 3 0.6299797674467946 1102 4 0.710366207416115 1102 5 0.683262719791685 1102 6 0.7316441928316237 1102 7 0.7406850044661439 1102 8 0.8370967843335606 1102 9 1.005177391197364 1102 10-11 1.0035619113038905 1102 12-13 1.0440081934618064 1102 14-15 1.1675072140817235 1102 16-17 1.0205815282758124 1102 18-19 0.9805548220090969 1102 20-21 1.023317018162345 1102 22-23 1.0611282408425353 1102 24-25 1.0762430383835664 1102 26-27 1.2106827051110471 1102 28-29 1.2523957348458126 1102 30-31 1.1540663082958034 1102 32-33 1.1928343345413097 1102 34-35 1.1108116673506458 1102 36-37 1.098032454279533 1102 38-39 1.0040635205413153 1102 40-41 1.0010735089126825 1102 42-43 0.9811796064531038 1102 44-45 1.111272721296423 1102 46-47 1.1009223117970208 1102 48-49 1.079444936991024 1102 50-51 1.0699930827197122 1102 52-53 1.0712130150594845 1102 54-55 1.090547182972827 1102 56-57 1.0919684915950327 1102 58-59 1.116857207787632 1102 60-61 1.139485819972485 1102 62-63 1.142905500134276 1102 64-65 1.1348777932001042 1102 66-67 1.108995817362711 1102 68-69 1.1069095103323718 1102 70-71 0.9914884292139803 1102 72-73 1.0038999539793778 1102 74-75 0.949231077147406 1102 76-77 1.0247700268055198 1102 78-79 0.8845738697128169 1102 80-81 0.8830974255253068 1102 82-83 0.8617564585058659 1102 84-85 0.8809995244214051 1102 86-87 0.8106254127035832 1102 88-89 0.9014252839008208 1102 90-91 0.9465959727383435 1102 92-93 0.873617497684954 1102 94-95 0.9270868701889441 1102 96-97 0.983060489501618 1102 98-99 0.9001576236050006 1102 100-101 0.7772625731169498 1102 102-103 0.6982819293298128 1102 104-105 0.9003638497867001 1102 106-107 0.8965957548996322 1102 108-109 0.8501451255729258 1102 110-111 0.9774695324424201 1102 112-113 1.000593211669269 1102 114-115 0.9478807496794701 1102 116-117 0.9014115330628627 1102 118-119 0.8072466061389854 1102 120-121 0.9327302081763058 1102 122-123 0.9319070824315787 1102 124-125 0.8462674256953377 1103 1 0.4172717313203691 1103 2 0.3709577464980818 1103 3 0.4545154336790773 1103 4 0.4927383032459218 1103 5 0.39966591553012876 1103 6 0.5445915491064923 1103 7 0.5412679821940003 1103 8 0.5898700835937021 1103 9 0.7040025433993335 1103 10-11 0.6852996867150836 1103 12-13 0.7105261956912443 1103 14-15 0.7835250220558265 1103 16-17 0.752654036858516 1103 18-19 0.6648603141949536 1103 20-21 0.7400470074809462 1103 22-23 0.8424692484084346 1103 24-25 0.8622401928340224 1103 26-27 0.887974309363706 1103 28-29 0.9577217715182584 1103 30-31 0.7768293284316599 1103 32-33 1.0176370886267634 1103 34-35 0.8423911273102291 1103 36-37 0.8564099405625711 1103 38-39 0.9041699110654662 1103 40-41 0.9454096161627987 1103 42-43 0.8626390727220254 1103 44-45 0.9158155437470441 1103 46-47 0.7983530600346995 1103 48-49 0.8055871530685863 1103 50-51 0.898662364864208 1103 52-53 0.9239393119388097 1103 54-55 1.0371879418707266 1103 56-57 1.034823071098394 1103 58-59 1.028517609234541 1103 60-61 0.9470794039357884 1103 62-63 1.0125257124283635 1103 64-65 0.8759494051382859 1103 66-67 1.0528651825024085 1103 68-69 1.0134431059192366 1103 70-71 1.0326502878405606 1103 72-73 1.0381130293218561 1103 74-75 0.9770362670994821 1103 76-77 0.8650312181888431 1103 78-79 0.8425248411482862 1103 80-81 0.8640695067462687 1103 82-83 0.8113079774555523 1103 84-85 0.8929496150915384 1103 86-87 0.8744945026041044 1103 88-89 0.8259694190305495 1103 90-91 0.8173312741728651 1103 92-93 0.7342264325834833 1103 94-95 0.7739969400255902 1103 96-97 0.8008044704974111 1103 98-99 0.7926289325683769 1103 100-101 0.5850419435179397 1103 102-103 0.5557504911945799 1103 104-105 0.7066378207362654 1103 106-107 0.6263514907250745 1103 108-109 0.6231891013815236 1103 110-111 0.6115813515298321 1103 112-113 0.5719781833990325 1103 114-115 0.5733576809321335 1103 116-117 0.5672472068112917 1103 118-119 0.5337095287766651 1103 120-121 0.5900393430466728 1103 122-123 0.5003376263571049 1103 124-125 0.6375322872870903 1104 1 0.3560260432845759 1104 2 0.3240837070841671 1104 3 0.32735838425798747 1104 4 0.42474759318069033 1104 5 0.3274023050926189 1104 6 0.3564392044225926 1104 7 0.4407059326021425 1104 8 0.5007735895194472 1104 9 0.5957060681098767 1104 10-11 0.4700886993179765 1104 12-13 0.5455327276766226 1104 14-15 0.5863216083463456 1104 16-17 0.6494382081075258 1104 18-19 0.6730134486940855 1104 20-21 0.522213193143017 1104 22-23 0.4708936310717746 1104 24-25 0.5173308721605068 1104 26-27 0.6433971380122259 1104 28-29 0.6373827486691184 1104 30-31 0.5624759309454106 1104 32-33 0.5760183089553266 1104 34-35 0.5018004949457335 1104 36-37 0.5037796423966512 1104 38-39 0.6042628744522034 1104 40-41 0.5846242623255833 1104 42-43 0.6197544540533428 1104 44-45 0.5145230218029937 1104 46-47 0.5439846347127997 1104 48-49 0.5415432777654701 1104 50-51 0.5396821657453827 1104 52-53 0.5456216610627536 1104 54-55 0.6269409431685915 1104 56-57 0.6238871187093054 1104 58-59 0.6767656069462049 1104 60-61 0.6270131875349492 1104 62-63 0.7470320992585116 1104 64-65 0.7527847649524873 1104 66-67 0.7041751017277846 1104 68-69 0.687464588893377 1104 70-71 0.6553908826354657 1104 72-73 0.5608829753582683 1104 74-75 0.6311507774994318 1104 76-77 0.48101761202059734 1104 78-79 0.486845109087767 1104 80-81 0.5720312711507916 1104 82-83 0.6331391106909763 1104 84-85 0.597828965479188 1104 86-87 0.4909154015419084 1104 88-89 0.5588338440450649 1104 90-91 0.5704706829641495 1104 92-93 0.5402502679262184 1104 94-95 0.566774933638758 1104 96-97 0.4512363073087755 1104 98-99 0.5211828876214852 1104 100-101 0.4053604738639258 1104 102-103 0.24525459396448213 1104 104-105 0.37593642212292266 1104 106-107 0.3474049761583231 1104 108-109 0.4223096314542687 1104 110-111 0.49929972476662243 1104 112-113 0.43451906167356213 1104 114-115 0.4289372985761801 1104 116-117 0.4292049026000839 1104 118-119 0.4486462154804478 1104 120-121 0.5464072816716268 1104 122-123 0.5451119313197061 1104 124-125 0.5870480451681637 1105 1 0.13238887043902636 1105 2 0.1997367504010228 1105 3 0.19484448388506337 1105 4 0.13953134640793508 1105 5 0.11499142356851166 1105 6 0.03696834669153759 1105 7 0.11488910112782236 1105 8 0.10834802717658931 1105 9 0.20350778424696614 1105 10-11 0.2515062842389142 1105 12-13 0.277034579653467 1105 14-15 0.2676922610521757 1105 16-17 0.312282553461138 1105 18-19 0.29790002038541274 1105 20-21 0.3035055903351065 1105 22-23 0.2450771159944125 1105 24-25 0.33810929304439696 1105 26-27 0.3995778470788949 1105 28-29 0.38014221526602654 1105 30-31 0.25366844161853663 1105 32-33 0.1395418531074668 1105 34-35 0.2773300907169798 1105 36-37 0.2412391435862986 1105 38-39 0.3090237521795416 1105 40-41 0.33829451974614244 1105 42-43 0.3449186640734254 1105 44-45 0.2815187061485105 1105 46-47 0.39997186704097487 1105 48-49 0.35126842372993394 1105 50-51 0.373017643546973 1105 52-53 0.3621534020053616 1105 54-55 0.4988139393212023 1105 56-57 0.4700478525815015 1105 58-59 0.4302837828966659 1105 60-61 0.52167251238604 1105 62-63 0.5977958815099029 1105 64-65 0.6904775561642467 1105 66-67 0.5851531585442444 1105 68-69 0.6194337591740009 1105 70-71 0.530094049558059 1105 72-73 0.4294230987108918 1105 74-75 0.436747101388665 1105 76-77 0.4361762007214267 1105 78-79 0.37264252433556777 1105 80-81 0.38900470224375283 1105 82-83 0.37673977132782 1105 84-85 0.38722945652726537 1105 86-87 0.3348715868914667 1105 88-89 0.35909518793058837 1105 90-91 0.3291702281962614 1105 92-93 0.2877382342953325 1105 94-95 0.3637756486958814 1105 96-97 0.3288348755070878 1105 98-99 0.2767718289150878 1105 100-101 0.3028855114096949 1105 102-103 0.17468420407906038 1105 104-105 0.28163357117542276 1105 106-107 0.2773416123946326 1105 108-109 0.29084374326473394 1105 110-111 0.2631938752197023 1105 112-113 0.3069123198872319 1105 114-115 0.37722037049531565 1105 116-117 0.26265475790120973 1105 118-119 0.28981589283992193 1105 120-121 0.18373721830786494 1105 122-123 0.155437598706353 1105 124-125 0.2003803273604987 1106 1 0.3001856836244734 1106 2 0.26526511805841224 1106 3 0.31745085181612254 1106 4 0.2744808123362077 1106 5 0.1987427141565803 1106 6 0.27973724144255385 1106 7 0.20832787779679762 1106 8 0.36021754907415726 1106 9 0.370156861865226 1106 10-11 0.3944489193298324 1106 12-13 0.39388140365316815 1106 14-15 0.3199084109285195 1106 16-17 0.32471939841120445 1106 18-19 0.46696003488735016 1106 20-21 0.4473856949544981 1106 22-23 0.438625499855128 1106 24-25 0.4170838528600669 1106 26-27 0.4988842600726926 1106 28-29 0.523262713757056 1106 30-31 0.44328973603072086 1106 32-33 0.18482825230556443 1106 34-35 0.41145133316456395 1106 36-37 0.4320592353088415 1106 38-39 0.3582701450536092 1106 40-41 0.418611030325053 1106 42-43 0.38221781285711387 1106 44-45 0.3582952535400281 1106 46-47 0.26754759993247745 1106 48-49 0.33207893911847464 1106 50-51 0.34650772071541525 1106 52-53 0.33709537820918456 1106 54-55 0.3544088718294134 1106 56-57 0.3106884657878908 1106 58-59 0.2670834796159909 1106 60-61 0.28752838745590736 1106 62-63 0.32449652847925137 1106 64-65 0.23304709944955704 1106 66-67 0.29094577724323756 1106 68-69 0.3473881775897709 1106 70-71 0.24644446112172602 1106 72-73 0.24767444166483443 1106 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-3.2944652129369345 2216 70-71 -3.285160298131302 2216 72-73 -3.038741535354898 2216 74-75 -3.061160173660536 2216 76-77 -3.0553043063247607 2216 78-79 -3.0730807221884078 2216 80-81 -3.103997254101184 2216 82-83 -3.2854225053535373 2216 84-85 -3.4004332990834882 2216 86-87 -3.357369025839059 2216 88-89 -3.379341610463193 2216 90-91 -3.4130549270248025 2216 92-93 -3.4456208075501102 2216 94-95 -3.401718105531465 2216 96-97 -3.365051062612526 2216 98-99 -3.3311400314603574 2216 100-101 -3.35159038264376 2216 102-103 -3.2780045592794735 2216 104-105 -3.427296706857856 2216 106-107 -3.3605098777034605 2216 108-109 -3.2618299738429855 2216 110-111 -3.326234484395826 2216 112-113 -3.4075628879043194 2216 114-115 -3.533871574393366 2216 116-117 -3.4937791759722217 2216 118-119 -3.515212280039254 2216 120-121 -3.540015818463395 2216 122-123 -3.510314592118327 2216 124-125 -3.6266595300831277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60967.0 3 11063.0 4 7188.0 5 5508.0 6 6156.0 7 6187.0 8 6402.0 9 7167.0 10 8616.0 11 10058.0 12 11308.0 13 12364.0 14 13133.0 15 12870.0 16 11810.0 17 11753.0 18 11923.0 19 12154.0 20 12277.0 21 13422.0 22 15476.0 23 19271.0 24 23174.0 25 25141.0 26 28833.0 27 31000.0 28 36140.0 29 44489.0 30 52959.0 31 62239.0 32 79190.0 33 102027.0 34 129946.0 35 172395.0 36 269844.0 37 501115.0 38 257005.0 39 1749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.778638055470005 18.65118644310588 11.552439137939679 23.017736363484435 2 13.845974620980995 22.453575722158085 38.49345376383514 25.206995893025784 3 21.058759505635354 29.10464433528578 28.011800592893866 21.824795566185003 4 12.98675012363878 18.16597384395354 34.70402117230568 34.143254860102004 5 12.053392834807982 40.9029673400683 33.46619004381455 13.577449781309166 6 30.757310364483452 32.68139075354842 19.890454932368595 16.67084394959953 7 26.38870392089193 33.89511097363812 21.71949113991361 17.996693965556336 8 27.807019637988738 34.177548808495 21.360621845770407 16.65480970774586 9 29.477479198252293 16.53763415187365 19.832439982331945 34.152446667542115 10-11 23.902610707148995 29.40305374825189 25.581439216217188 21.11289632838192 12-13 25.870804283840616 27.053389941193057 25.524753283759672 21.551052491206658 14-15 25.775159471948644 26.476568687878032 23.904321127640102 23.843950712533225 16-17 22.50165968378041 29.916322336491007 27.863470747909254 19.71854723181933 18-19 23.56268175924812 28.913969994328824 28.28431681436545 19.239031432057605 20-21 22.335536602191812 28.97420289647501 28.044175182551445 20.646085318781733 22-23 21.427423738921853 29.991054159935004 24.686985127749793 23.89453697339335 24-25 21.708001554534263 30.888419197161355 24.14199830223469 23.261580946069692 26-27 23.028120492248277 28.174137627255575 24.851991754798362 23.945750125697785 28-29 22.601183723515188 27.977738816887637 27.010257791223104 22.41081966837407 30-31 24.186586557332898 29.25829147840305 25.87701956604579 20.678102398218265 32-33 20.496777405187427 29.516700311217065 27.40028101456525 22.58624126903026 34-35 19.501485459117166 30.304535831031053 27.883108548814832 22.31087016103695 36-37 23.30488068549817 28.899921104517663 26.090325542901628 21.70487266708254 38-39 24.608223606680642 27.823519083801934 25.751610474597392 21.816646834920032 40-41 21.887830412185714 30.021315837934747 25.749513142063286 22.34134060781625 42-43 23.763953326373766 28.935820901219323 25.472872004412388 21.82735376799452 44-45 24.886689186044986 27.73825816466669 25.476122578281885 21.89893007100644 46-47 22.317316017729276 28.859806803222426 28.611700061000466 20.211177118047832 48-49 23.511289885699078 28.454425804634802 27.93257212269898 20.10171218696714 50-51 21.031285367574615 29.350299667677533 28.046517987338483 21.571896977409374 52-53 20.55427929518614 30.210586142325674 25.039059293773608 24.19607526871458 54-55 21.235558915140416 30.623832914101044 24.400321260302448 23.74028691045609 56-57 22.67233463501937 28.262801416976185 24.856652709863177 24.20821123814127 58-59 22.549205323703728 27.901323577758703 26.729456965233123 22.82001413330445 60-61 23.719200897700464 29.19621402511433 25.978674603384793 21.105910473800407 62-63 20.330395834595933 29.484926847262315 27.37624114372047 22.808436174421285 64-65 19.157690013124924 29.973249015630593 27.969502446008832 22.89955852523565 66-67 22.737435366374527 29.009814050819273 25.98494844819646 22.26780213460974 68-69 24.020862558660436 27.913049765238945 25.69188115939055 22.374206516710064 70-71 21.392878228095437 29.817342859065587 25.78531688777928 23.004462025059695 72-73 23.327897931214203 28.942860026980533 25.522088549883552 22.207153491921712 74-75 24.34449268853099 27.711104736548947 25.556969414352217 22.38743316056785 76-77 21.925337735534338 28.736269072605058 28.60908058953413 20.729312602326473 78-79 23.193650342188413 28.520274323389884 27.732866360643342 20.55320897377836 80-81 20.800823545497956 29.40803372069466 27.73070883089804 22.06043390290935 82-83 20.355796257104696 30.140909196120084 24.980021299478157 24.52327324729706 84-85 21.058222824393273 30.588303511390052 24.322378603222994 24.03109506099368 86-87 22.279075239354995 28.14496389838715 25.06335112979661 24.51260973246124 88-89 22.35845655386235 27.70664626134229 26.763663538343373 23.171233646451984 90-91 23.349269555357452 29.023029097524333 25.952069163649 21.675632183469215 92-93 20.38530586764456 29.211197070318047 27.06684112522429 23.336655936813102 94-95 19.36711893479235 29.64433102609614 27.726721491191437 23.261828547920075 96-97 22.722202262780446 28.58996801877173 26.131044311392102 22.556785407055717 98-99 23.70964199167041 27.676798704890025 25.65264083998986 22.960918463449705 100-101 21.452664471087445 29.24926025007089 25.7093836327511 23.588691646090567 102-103 23.03990320945358 28.536090674848907 25.631897387523196 22.792108728174316 104-105 23.95378854700133 27.312581625823057 25.739356779572304 22.994273047603308 106-107 21.770020031419683 28.36984692980751 28.432452569371225 21.427680469401583 108-109 22.719874446050444 28.445315816840065 27.521502067919347 21.31330766919015 110-111 20.60833553995415 29.300799393119636 27.660075430648888 22.430789636277325 112-113 20.140053276159385 29.943878024454936 25.088075982575873 24.827992716809806 114-115 20.653291432557133 30.8075186671393 24.443468540783364 24.095721359520205 116-117 21.92284725223047 28.606450492834746 24.920825928649855 24.549876326284934 118-119 21.773139654096035 28.30840589781724 26.341814516206014 23.57663993188071 120-121 22.561249778045926 29.560344778208215 25.692090988760054 22.186314454985805 122-123 20.22677816322665 29.729590466271212 26.567807062031928 23.475824308470212 124-125 19.474889335699324 30.408357650417457 26.9592238697595 23.157529144123718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9444.0 1 5680.0 2 2841.5 3 3825.0 4 3684.5 5 3074.5 6 2402.5 7 2057.0 8 1972.0 9 1853.0 10 1628.0 11 1506.0 12 1484.5 13 1510.5 14 1590.5 15 1683.0 16 1840.5 17 2030.0 18 2230.0 19 2568.5 20 3126.5 21 3826.0 22 4630.5 23 5833.0 24 7503.0 25 9461.0 26 12146.5 27 14864.0 28 16727.5 29 19130.5 30 23017.5 31 26604.5 32 29534.0 33 33376.0 34 38323.0 35 42572.0 36 47286.5 37 51308.5 38 51893.5 39 51824.5 40 51949.0 41 53075.5 42 53972.0 43 55496.5 44 58086.0 45 59834.5 46 62190.5 47 67514.0 48 77193.5 49 106729.5 50 202648.5 51 208769.0 52 106561.5 53 62386.5 54 50455.0 55 43623.0 56 37727.0 57 32967.5 58 30209.0 59 27279.0 60 23494.5 61 19696.0 62 16084.0 63 12214.0 64 9149.5 65 7315.0 66 5470.0 67 4118.0 68 3130.5 69 2221.0 70 1807.0 71 1562.0 72 1408.5 73 1342.5 74 1029.5 75 799.5 76 568.5 77 311.5 78 208.0 79 137.0 80 93.5 81 66.0 82 44.0 83 32.0 84 27.5 85 29.5 86 22.0 87 14.5 88 15.0 89 15.0 90 11.5 91 8.0 92 6.5 93 4.5 94 5.0 95 6.0 96 3.0 97 0.5 98 2.5 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47682884581662766 2 0.46727706208041647 3 0.4518801569533897 4 0.4517851143291488 5 0.4553016914260623 6 0.45886578983509624 7 0.444894524071683 8 0.47088868180157095 9 0.4820561901498775 10-11 0.48802011482099433 12-13 0.4877349869482716 14-15 0.46333279317441894 16-17 0.46539997025165863 18-19 0.4521652848261124 20-21 0.4611468128168781 22-23 0.4775654261544946 24-25 0.465756380092562 26-27 0.4756883343257367 28-29 0.4709837244258119 30-31 0.4838144786983342 32-33 0.4809869606271673 34-35 0.4760209835105799 36-37 0.4344398354051833 38-39 0.3969455201421458 40-41 0.3908627921907277 42-43 0.39932158574816845 44-45 0.4318024025824982 46-47 0.4397147010505536 48-49 0.43679214035514574 50-51 0.42362873689778024 52-53 0.4175697696024224 54-55 0.41923301552663833 56-57 0.4014362841375286 58-59 0.4067824317510796 60-61 0.41545507121306224 62-63 0.41685694992061567 64-65 0.43097077962039027 66-67 0.42075369751449276 68-69 0.42248822540688935 70-71 0.4315885566779562 72-73 0.43265778620066636 74-75 0.43653077313848326 76-77 0.42785813367650055 78-79 0.4198983138963246 80-81 0.42714531399469374 82-83 0.425909759879562 84-85 0.4247454877326109 86-87 0.4301866779704028 88-89 0.41759353025848267 90-91 0.422131815565986 92-93 0.43123214683705274 94-95 0.42902240582345164 96-97 0.39927406443604796 98-99 0.37149785750164305 100-101 0.3722581984955703 102-103 0.3526081359337629 104-105 0.3554356540049298 106-107 0.3736363165470635 108-109 0.38059818877270984 110-111 0.3969930414542662 112-113 0.39250227745888333 114-115 0.3923359528664618 116-117 0.36463102790023755 118-119 0.4358179534566765 120-121 0.44152051091113087 122-123 0.3968029562057844 124-125 0.3909815954710289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2104319.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.58979915290832 #Duplication Level Percentage of deduplicated Percentage of total 1 84.69567438817545 43.694328308060925 2 9.634117687603048 9.940443930378455 3 2.5503293163241234 3.9471293160880645 4 1.0186448968991397 2.1020674255664646 5 0.5113647566024986 1.3190602543499372 6 0.2991075690046044 0.9258539646037324 7 0.2186626793666719 0.7896534597534376 8 0.13722986804675003 0.5663729064249555 9 0.10557164390330794 0.4901777914692614 >10 0.6994639869212841 7.057961494110295 >50 0.08053284823238106 2.8582509516108088 >100 0.042897715548242225 3.804275621842499 >500 0.002240925180409329 0.8046378046191244 >1k 0.0016006608431495205 2.3851763494066898 >5k 0.0012805286745196163 4.338801265424674 >10k+ 0.0012805286745196163 14.975809156290708 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 62928 2.99042112911588 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 30426 1.4458834425769098 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17580 0.8354246670775676 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16009 0.7607686857363356 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 15802 0.7509317741274018 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14856 0.7059766128614531 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12749 0.6058492082236581 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11004 0.5229245185734672 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7985 0.37945767728181895 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7687 0.3652963262699239 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6933 0.3294652569311022 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6439 0.30598972874359825 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6195 0.2943945285862077 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6116 0.2906403449286919 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5698 0.27077643646234245 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 5489 0.26084448222916773 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4823 0.2291952883569459 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4336 0.20605240935428515 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4257 0.20229822569676936 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3454 0.16413861206404542 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 2781 0.1321567690069804 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2517 0.11961114260718075 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2431 0.11552430976482178 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.752131212045322E-5 0.0 0.0 0.0 0.0 3 4.752131212045322E-5 0.0 0.0 0.0 0.0 4 4.752131212045322E-5 0.0 0.0 0.0 0.0 5 9.504262424090645E-5 0.0 0.0 0.0 0.0 6 9.504262424090645E-5 0.0 0.0 0.0 0.0 7 9.504262424090645E-5 0.0 0.0 4.752131212045322E-5 0.0 8 9.504262424090645E-5 0.0 0.0 4.752131212045322E-5 0.0 9 9.504262424090645E-5 0.0 0.0 4.752131212045322E-5 0.0 10-11 9.504262424090645E-5 0.0 0.0 1.900852484818129E-4 0.0 12-13 1.4256393636135966E-4 0.0 0.0 5.940164015056652E-4 0.0 14-15 2.613672166624927E-4 0.0 0.0 0.0013068360833124637 0.0 16-17 3.088885287829459E-4 0.0 0.0 0.002185980357540848 0.0 18-19 4.27691809084079E-4 0.0 0.0 0.0030651246317692326 0.0 20-21 5.227344333249854E-4 0.0 0.0 0.004514524651443056 0.0 22-23 5.227344333249854E-4 0.0 0.0 0.007413324690790701 0.0 24-25 6.177770575658918E-4 0.0 0.0 0.010217082105897442 0.0 26-27 6.177770575658918E-4 0.0 0.0 0.013020839521004182 0.0 28-29 7.841016499874781E-4 0.0 0.0 0.017226475643664294 0.0 30-31 9.029049302886112E-4 0.0 0.0 0.024924928207177715 0.0 32-33 9.029049302886112E-4 0.0 0.0 0.043149351405371524 0.0 34-35 9.504262424090644E-4 0.0 0.0 0.07002265340948782 0.0 36-37 9.979475545295177E-4 0.0 0.0 0.1129581589103173 0.0 38-39 0.0011405114908908773 0.0 0.0 0.1786563729168439 0.0 40-41 0.0011405114908908773 0.0 0.0 0.26445610195032215 0.0 42-43 0.00133059673937269 0.0 0.0 0.3360231980037247 0.0 44-45 0.0013781180514931433 0.0 0.0 0.41621541220698954 0.0 46-47 0.0016394852681556361 0.0 0.0 0.5189802496674696 0.0 48-49 0.0018058098605772223 0.0 0.0 0.618846287088602 0.0 50-51 0.0018058098605772223 0.0 0.0 0.7197577933763845 0.0 52-53 0.0018058098605772223 0.0 0.0 0.8331436440957858 0.0 54-55 0.0018533311726976755 0.0 0.0 0.9612611015725276 0.0 56-57 0.001948373796938582 0.0 0.0 1.1129491298610144 0.0 58-59 0.001948373796938582 0.0 0.0 1.2876612338718607 0.0 60-61 0.001948373796938582 0.0 0.0 1.4556728328737232 0.0 62-63 0.001948373796938582 0.0 0.0 1.6232805007225615 0.0 64-65 0.001948373796938582 0.0 0.0 1.8098254114514007 0.0 66-67 0.0020434164211794886 0.0 0.0 2.0303005390342435 0.0 68-69 0.0020434164211794886 0.0 0.0 2.284895968719572 0.0 70-71 0.0020909377332999418 0.0 0.0 2.628047363541364 0.0 72-73 0.002162219701480622 0.0 0.0 2.9124148952701563 0.0 74-75 0.002185980357540848 0.0 0.0 3.195285505667154 0.0 76-77 0.002185980357540848 0.0 0.0 3.5341837430541663 0.0 78-79 0.002185980357540848 0.0 0.0 3.8835841904197985 0.0 80-81 0.002613672166624927 0.0 0.0 4.250852651142722 0.0 82-83 0.00266119347874538 0.0 0.0 4.669182761739071 0.0 84-85 0.0027324754469260602 0.0 0.0 5.072377334425056 0.0 86-87 0.00280375741510674 0.0 0.0 5.495530858201632 0.0 88-89 0.0028275180711669666 0.0 0.0 5.978252346721195 0.0 90-91 0.0028988000393476462 0.0 0.0 6.457172130271124 0.0 92-93 0.0028988000393476462 0.0 0.0 6.952985740279872 0.0 94-95 0.0028988000393476462 0.0 0.0 7.480424783504782 0.0 96-97 0.0028988000393476462 0.0 0.0 8.010643823488738 0.0 98-99 0.0029700820075283267 0.0 0.0 8.624737979365296 0.0 100-101 0.0030413639757090063 0.0 0.0 9.409552449034582 0.0 102-103 0.0030888852878294595 4.752131212045322E-5 0.0 10.105050612573475 0.0 104-105 0.003587859065094218 7.128196818067983E-5 0.0 10.763719759219015 0.0 106-107 0.0037779443135760307 9.504262424090645E-5 0.0 11.445389220930856 0.0 108-109 0.0038492262817567107 9.504262424090645E-5 0.0 12.1044147774173 0.0 110-111 0.003896747593877164 9.504262424090645E-5 0.0 12.811270534552984 0.0 112-113 0.003920508249937391 9.504262424090645E-5 0.0 13.615402417599233 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1455 0.0 32.73267 5 CATGGGG 7330 0.0 25.419914 4 AAGTACT 2000 0.0 22.918936 4 ATGGGAG 3555 0.0 22.272373 5 CATGGGT 5175 0.0 21.511217 4 ATGGGGA 3860 0.0 21.283655 5 ATGGGGG 2870 0.0 20.328186 5 GGGTACC 755 0.0 18.921993 7 ATGGGAT 3415 0.0 18.827276 5 CGTACAC 190 8.298259E-4 18.798405 3 AAAGTAC 2420 0.0 18.694832 3 ATGGGTA 3250 0.0 18.317705 5 ATGGGGT 1515 0.0 17.682934 5 GTACAAA 2320 0.0 16.675793 1 TGGGTAC 1060 0.0 16.285261 6 GTATTAC 330 7.4875607E-6 16.23266 1 GTATAGG 665 0.0 16.110611 1 GGGTGCG 225 0.002575725 15.87345 7 ATGGGTG 2080 0.0 15.741779 5 GTACATA 1215 0.0 15.675986 1 >>END_MODULE