##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364141_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104319 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06079068810385 33.0 31.0 34.0 30.0 34.0 2 31.89027091424827 33.0 31.0 34.0 30.0 34.0 3 32.11461570227708 34.0 31.0 34.0 30.0 34.0 4 35.60474766420871 37.0 35.0 37.0 33.0 37.0 5 35.73706030311944 37.0 35.0 37.0 33.0 37.0 6 35.86976451764205 37.0 35.0 37.0 35.0 37.0 7 35.84991439035622 37.0 35.0 37.0 35.0 37.0 8 35.89186097735182 37.0 35.0 37.0 35.0 37.0 9 37.54743553615208 39.0 37.0 39.0 35.0 39.0 10-11 37.51335895365674 39.0 37.0 39.0 34.5 39.0 12-13 37.49238162084741 39.0 37.0 39.0 34.5 39.0 14-15 38.76943847391959 40.0 38.0 41.0 34.0 41.0 16-17 38.7583450988182 40.0 38.0 41.0 34.0 41.0 18-19 38.866687987895375 40.0 38.0 41.0 34.5 41.0 20-21 38.93397864107105 40.0 38.5 41.0 35.0 41.0 22-23 38.90876858499115 40.0 38.0 41.0 35.0 41.0 24-25 38.87759032732205 40.0 38.0 41.0 35.0 41.0 26-27 38.715163432920576 40.0 38.0 41.0 34.0 41.0 28-29 38.57858932034544 40.0 38.0 41.0 34.0 41.0 30-31 38.44528586207699 40.0 38.0 41.0 34.0 41.0 32-33 38.19249838071129 40.0 37.0 41.0 34.0 41.0 34-35 38.032736006280416 40.0 37.0 41.0 33.5 41.0 36-37 37.93857086306782 40.0 37.0 41.0 33.0 41.0 38-39 37.846860195626235 40.0 37.0 41.0 33.0 41.0 40-41 37.650551318502565 40.0 37.0 41.0 33.0 41.0 42-43 37.480340195569205 40.0 37.0 41.0 33.0 41.0 44-45 37.231722946948636 40.0 36.5 41.0 32.5 41.0 46-47 36.867641503023066 40.0 36.0 41.0 31.0 41.0 48-49 36.51307026168561 40.0 36.0 41.0 30.0 41.0 50-51 36.32014585241116 40.0 36.0 41.0 30.0 41.0 52-53 36.200165469208805 40.0 35.5 41.0 29.5 41.0 54-55 36.01997510833671 39.0 35.0 41.0 29.0 41.0 56-57 35.77846324630438 39.0 35.0 41.0 28.0 41.0 58-59 35.6287630345019 39.0 35.0 41.0 28.0 41.0 60-61 35.467690497495866 39.0 35.0 41.0 28.0 41.0 62-63 35.15827970949272 38.5 35.0 40.0 28.0 41.0 64-65 34.88565659484137 38.0 35.0 40.0 28.0 41.0 66-67 34.50233282121199 37.0 34.5 40.0 26.5 41.0 68-69 34.1736129360615 37.0 34.0 39.5 26.0 41.0 70-71 33.803142964541024 36.0 34.0 39.0 26.0 41.0 72-73 33.50995476446299 36.0 34.0 39.0 26.0 40.5 74-75 33.14478840898172 35.0 34.0 37.5 26.0 39.5 76-77 32.72625015503828 35.0 33.5 37.0 26.0 39.0 78-79 32.373594022579276 35.0 33.0 36.5 25.0 39.0 80-81 32.095554903985565 35.0 33.0 36.0 24.5 38.0 82-83 31.886533838263112 35.0 33.0 36.0 24.0 37.0 84-85 31.63676871234827 35.0 33.0 35.0 24.0 37.0 86-87 31.37735628485985 35.0 33.0 35.0 23.0 36.0 88-89 31.200573439673356 35.0 33.0 35.0 20.5 36.0 90-91 31.040689173076895 35.0 33.0 35.0 20.0 36.0 92-93 30.8595899671105 35.0 32.0 35.0 19.0 36.0 94-95 30.78092294941974 35.0 32.0 35.0 19.0 35.0 96-97 30.707244006255706 35.0 32.0 35.0 18.5 35.0 98-99 30.605310078937652 35.0 32.0 35.0 17.5 35.0 100-101 30.453626565173817 35.0 32.0 35.0 14.0 35.0 102-103 29.199195559228425 33.5 29.5 34.5 7.5 35.0 104-105 30.030266086082957 34.5 31.0 35.0 7.0 35.0 106-107 30.125416346095818 35.0 32.0 35.0 7.0 35.0 108-109 30.09845988179549 35.0 32.0 35.0 3.5 35.0 110-111 30.057392439074114 35.0 32.0 35.0 2.0 35.0 112-113 30.017731627191505 35.0 32.0 35.0 2.0 35.0 114-115 29.91052924960522 35.0 32.0 35.0 2.0 35.0 116-117 29.719896318001215 35.0 32.0 35.0 2.0 35.0 118-119 29.58707496344423 35.0 32.0 35.0 2.0 35.0 120-121 29.422763611410627 35.0 31.0 35.0 2.0 35.0 122-123 29.15276224754897 35.0 31.0 35.0 2.0 35.0 124-125 28.170418078247643 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38457038074742655 1101 2 0.6542168477368229 1101 3 0.47449178033947703 1101 4 0.4796476836287411 1101 5 0.2550790356337558 1101 6 0.18718121416885936 1101 7 0.20671222943132506 1101 8 0.21912190543758214 1101 9 0.30194920769506695 1101 10-11 0.2935864105505601 1101 12-13 0.2564461743055375 1101 14-15 0.3959153389846293 1101 16-17 0.36440070636749056 1101 18-19 0.2595997584487151 1101 20-21 0.26550616097532753 1101 22-23 0.28101507367712486 1101 24-25 0.3427219767750529 1101 26-27 0.3845831674069373 1101 28-29 0.3910839821940897 1101 30-31 0.3193880011922019 1101 32-33 0.3164548577624444 1101 34-35 0.3132335039973313 1101 36-37 0.3559603830157556 1101 38-39 0.30141799029755134 1101 40-41 0.34799229306863566 1101 42-43 0.29335647886183835 1101 44-45 0.3082674917165775 1101 46-47 0.1771222432909738 1101 48-49 0.16574749179491732 1101 50-51 0.15129788464936667 1101 52-53 0.17870453856082946 1101 54-55 0.19292118976856898 1101 56-57 0.1990123480140653 1101 58-59 0.20526641900386267 1101 60-61 0.16081336811296865 1101 62-63 0.13058050440136526 1101 64-65 0.07111207488648574 1101 66-67 0.15540758689898837 1101 68-69 0.04193087007801921 1101 70-71 0.040750939901315064 1101 72-73 0.07308852791800291 1101 74-75 0.06875164137674972 1101 76-77 -0.055582498718209195 1101 78-79 -0.02661555062196186 1101 80-81 -0.04340967931213413 1101 82-83 0.013923071882302906 1101 84-85 0.03726411615436831 1101 86-87 0.023727038831953706 1101 88-89 -0.05687067099767873 1101 90-91 0.03150109142513102 1101 92-93 0.06640338963392267 1101 94-95 0.009001089409665042 1101 96-97 0.08625663985253595 1101 98-99 0.07725851448238075 1101 100-101 0.06686793299802574 1101 102-103 -0.01373480544578598 1101 104-105 0.029751087616119776 1101 106-107 -0.022723290171501986 1101 108-109 -0.06663364889404377 1101 110-111 -0.06536336040502988 1101 112-113 0.056512265531630135 1101 114-115 0.038826369451914644 1101 116-117 0.08376727855758048 1101 118-119 0.14032330066697796 1101 120-121 0.06200387142296648 1101 122-123 0.14611884791661112 1101 124-125 0.18255701835455085 1102 1 0.3370796009169865 1102 2 0.6170167330296117 1102 3 0.4383748714256086 1102 4 0.5193183100750574 1102 5 0.2807677778356066 1102 6 0.2265159123639009 1102 7 0.2058389000126084 1102 8 0.22966131293073744 1102 9 0.30062492768763605 1102 10-11 0.2343868819845767 1102 12-13 0.23913074313984595 1102 14-15 0.38254853537660694 1102 16-17 0.35416145990561176 1102 18-19 0.3652058631410995 1102 20-21 0.3156849445537091 1102 22-23 0.3015932001050601 1102 24-25 0.318897436785619 1102 26-27 0.3603307338178894 1102 28-29 0.4750426728932382 1102 30-31 0.5170693819644541 1102 32-33 0.5210176730890836 1102 34-35 0.5289804821661974 1102 36-37 0.5077359287415959 1102 38-39 0.5178500078944026 1102 40-41 0.48312436917456125 1102 42-43 0.3855349599157165 1102 44-45 0.4769465941011646 1102 46-47 0.4719288534513879 1102 48-49 0.5607913946131191 1102 50-51 0.6166385030808783 1102 52-53 0.5662872207136687 1102 54-55 0.4933263415087623 1102 56-57 0.5724223348941564 1102 58-59 0.5585273285311771 1102 60-61 0.5519744529574382 1102 62-63 0.5523321738009059 1102 64-65 0.6916984997296254 1102 66-67 0.6138964454887414 1102 68-69 0.7135111614469309 1102 70-71 0.6230773638410909 1102 72-73 0.5754713285596083 1102 74-75 0.5570634811143904 1102 76-77 0.5375485143502203 1102 78-79 0.48584992954640427 1102 80-81 0.5661671120163447 1102 82-83 0.5272364610075968 1102 84-85 0.4876738897967101 1102 86-87 0.5740391222698946 1102 88-89 0.5546905445205681 1102 90-91 0.582191488057866 1102 92-93 0.5841051698015498 1102 94-95 0.6738487399602526 1102 96-97 0.5980238976674919 1102 98-99 0.5388560224367751 1102 100-101 0.5753243857511592 1102 102-103 0.5024258329649527 1102 104-105 0.48139821799262705 1102 106-107 0.4199299862844228 1102 108-109 0.49358521918111364 1102 110-111 0.5200107353040622 1102 112-113 0.49902289328048255 1102 114-115 0.5913533419274373 1102 116-117 0.647076203345577 1102 118-119 0.7398185679442228 1102 120-121 0.6928164648457908 1102 122-123 0.7482817405550755 1102 124-125 0.7852356207333564 1103 1 0.22032462835092304 1103 2 0.5781145350331478 1103 3 0.4210282405118093 1103 4 0.4816274819968527 1103 5 0.1534714627587448 1103 6 0.19934832452820217 1103 7 0.21234491875215866 1103 8 0.1738652524501063 1103 9 0.28035716232267305 1103 10-11 0.2400005435864898 1103 12-13 0.20732362863082443 1103 14-15 0.3868005570612141 1103 16-17 0.43230720539618517 1103 18-19 0.26100354329836506 1103 20-21 0.1369046139914687 1103 22-23 0.21359945015137782 1103 24-25 0.1740159325400512 1103 26-27 0.16499572011415609 1103 28-29 0.25062641811493336 1103 30-31 0.2033795680367163 1103 32-33 0.19230472319214442 1103 34-35 0.22583656278163033 1103 36-37 0.2165799454126187 1103 38-39 0.1627152095123776 1103 40-41 0.20714152716077905 1103 42-43 0.2280848708334986 1103 44-45 0.30338892673192674 1103 46-47 0.21664341455606717 1103 48-49 0.20626148723110305 1103 50-51 0.2813643278191691 1103 52-53 0.27721977335580306 1103 54-55 0.24944374371682443 1103 56-57 0.2657851011748278 1103 58-59 0.18908570411195313 1103 60-61 0.25709871814825647 1103 62-63 0.20358546476152384 1103 64-65 0.15523417972386966 1103 66-67 0.22842748558579018 1103 68-69 0.22096765513283856 1103 70-71 0.27393759209904545 1103 72-73 0.18552168674104053 1103 74-75 0.31134212689534735 1103 76-77 0.30867825468324384 1103 78-79 0.1673823341945564 1103 80-81 0.19473524376697782 1103 82-83 0.1929075205619455 1103 84-85 0.17765555463374483 1103 86-87 0.21907814800793446 1103 88-89 0.1653119710428257 1103 90-91 0.1027114677748564 1103 92-93 -0.04304293957002514 1103 94-95 0.11492845028161724 1103 96-97 0.08345199514026191 1103 98-99 0.1385357393215365 1103 100-101 0.1414398506989194 1103 102-103 0.016992601223101644 1103 104-105 0.17758954308925468 1103 106-107 0.2893370999465539 1103 108-109 0.22081590030277454 1103 110-111 0.2455063510617137 1103 112-113 0.2911720665572979 1103 114-115 0.20239519627722302 1103 116-117 0.33186405217152526 1103 118-119 0.2849781166790244 1103 120-121 0.37315417271752693 1103 122-123 0.39481388584324506 1103 124-125 0.5139939850928492 1104 1 0.20453259310381355 1104 2 0.5515835742762896 1104 3 0.1278206514548046 1104 4 0.42858840108561935 1104 5 0.2095445014554258 1104 6 0.22406356411971728 1104 7 0.1878766565164227 1104 8 0.199361128333841 1104 9 0.3217671746181736 1104 10-11 0.23724035283544254 1104 12-13 0.23696816908644536 1104 14-15 0.28674516326361754 1104 16-17 0.36046699995883813 1104 18-19 0.24714836979516974 1104 20-21 0.17509911773134945 1104 22-23 0.20519069302169157 1104 24-25 0.2431354379876467 1104 26-27 0.29346855900892166 1104 28-29 0.35652046760432654 1104 30-31 0.33275823961020734 1104 32-33 0.3733267440944914 1104 34-35 0.40674245949941223 1104 36-37 0.3873521925863628 1104 38-39 0.4542303526183602 1104 40-41 0.4332206922620472 1104 42-43 0.5128188098341582 1104 44-45 0.5413354925299387 1104 46-47 0.5680635877177167 1104 48-49 0.56439103400524 1104 50-51 0.5972281935591184 1104 52-53 0.6655354176742563 1104 54-55 0.5993955009489724 1104 56-57 0.694179278742368 1104 58-59 0.7530412054952009 1104 60-61 0.6036806326603639 1104 62-63 0.6264698272739011 1104 64-65 0.6363594102645322 1104 66-67 0.6940605972016272 1104 68-69 0.7149329331477929 1104 70-71 0.6489330026321909 1104 72-73 0.6084264350247963 1104 74-75 0.6508488979599392 1104 76-77 0.6338227695232277 1104 78-79 0.5564367759446185 1104 80-81 0.5675762731737208 1104 82-83 0.5276920504297635 1104 84-85 0.4627205756385635 1104 86-87 0.5389238565362291 1104 88-89 0.5215368890728129 1104 90-91 0.5480861390409508 1104 92-93 0.5079782701707423 1104 94-95 0.4211844272617036 1104 96-97 0.4029438877937004 1104 98-99 0.45253980366799595 1104 100-101 0.46465881736444814 1104 102-103 0.38772247587729325 1104 104-105 0.5559039920116895 1104 106-107 0.5559338928698097 1104 108-109 0.5536060399933369 1104 110-111 0.5954182973372042 1104 112-113 0.6465152732925006 1104 114-115 0.5982268930564842 1104 116-117 0.4360680038092184 1104 118-119 0.4625344497163546 1104 120-121 0.42496583807495014 1104 122-123 0.35234904329397665 1104 124-125 0.4590682063777294 1105 1 0.13101248713192604 1105 2 0.43065581402924735 1105 3 0.04079334850683125 1105 4 0.3446241370671217 1105 5 0.07299622349858481 1105 6 0.04296448546765674 1105 7 0.06764950459526631 1105 8 0.0888351289413265 1105 9 0.09160009593235685 1105 10-11 0.08647504133209338 1105 12-13 0.1005287316824095 1105 14-15 0.20069642336722637 1105 16-17 0.20191885067010418 1105 18-19 0.11955428388706935 1105 20-21 0.059417799073216315 1105 22-23 0.048389424058967734 1105 24-25 0.11299926073785116 1105 26-27 0.1494181928321794 1105 28-29 0.22651564782403 1105 30-31 0.20820778695162545 1105 32-33 0.1487822502452545 1105 34-35 0.24536514136438115 1105 36-37 0.23636297750392998 1105 38-39 0.21383336935786446 1105 40-41 0.17302738426860031 1105 42-43 0.17393615513713456 1105 44-45 0.27466917741544705 1105 46-47 0.3576360932441247 1105 48-49 0.36448595309198595 1105 50-51 0.3736682743036184 1105 52-53 0.3225926517742579 1105 54-55 0.3693915776267147 1105 56-57 0.42627780264509596 1105 58-59 0.4536873408118538 1105 60-61 0.48382329604775265 1105 62-63 0.410691427483151 1105 64-65 0.4321661486759609 1105 66-67 0.3974841704622847 1105 68-69 0.4896266081617142 1105 70-71 0.43376076667600216 1105 72-73 0.387816179122936 1105 74-75 0.2909164592565716 1105 76-77 0.22462922317740208 1105 78-79 0.32708062265662363 1105 80-81 0.2981859912007536 1105 82-83 0.30232091680056783 1105 84-85 0.338470120026944 1105 86-87 0.3381048102832125 1105 88-89 0.31887783632311084 1105 90-91 0.33157570216003407 1105 92-93 0.2971541193801741 1105 94-95 0.16961499289508808 1105 96-97 0.22925614416804763 1105 98-99 0.22902813494826546 1105 100-101 0.2683908776570547 1105 102-103 0.04364036453931419 1105 104-105 0.19153777730049626 1105 106-107 0.17621852357128276 1105 108-109 0.1879357424958279 1105 110-111 0.16434025908269945 1105 112-113 0.21819764027922162 1105 114-115 0.27014318285921846 1105 116-117 0.18054982996807212 1105 118-119 0.2204272354438963 1105 120-121 0.1333468770381785 1105 122-123 0.17647425844390696 1105 124-125 0.10059493770187089 1106 1 0.13160426163685202 1106 2 0.44913314281590644 1106 3 0.34390819509488324 1106 4 0.41898387898562106 1106 5 0.1911176850401688 1106 6 0.11158003207308553 1106 7 0.07683118849719506 1106 8 0.026241356909039837 1106 9 0.165622569519698 1106 10-11 0.0941006963569464 1106 12-13 0.1031635778210429 1106 14-15 0.18360904739509465 1106 16-17 0.13432268304954675 1106 18-19 0.09455775488179086 1106 20-21 0.12742336349909067 1106 22-23 0.0492507022437394 1106 24-25 -0.0024923203945448336 1106 26-27 0.034538399599135516 1106 28-29 0.11760566456105437 1106 30-31 0.09782744618421191 1106 32-33 0.12112950741707351 1106 34-35 0.07562090953563683 1106 36-37 0.01683003495511315 1106 38-39 0.0061840694354344805 1106 40-41 0.014296337805824066 1106 42-43 0.06432068718083883 1106 44-45 0.13430285304637835 1106 46-47 0.08880373988161239 1106 48-49 0.05287499421865505 1106 50-51 0.10561799824918694 1106 52-53 -0.07664271617726826 1106 54-55 -0.006510730524588837 1106 56-57 0.0390933571956964 1106 58-59 -0.0074577323218036895 1106 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-0.9173231600074594 2216 88-89 -0.9548200294771263 2216 90-91 -1.014837511754866 2216 92-93 -1.028125301793633 2216 94-95 -1.0587139817889124 2216 96-97 -1.066968850371353 2216 98-99 -1.082593718788054 2216 100-101 -1.169996968494992 2216 102-103 -1.1289255512909122 2216 104-105 -1.1853035917637627 2216 106-107 -1.3098216546540904 2216 108-109 -1.2989087579499525 2216 110-111 -1.254959536093871 2216 112-113 -1.3257628768963947 2216 114-115 -1.4812209288499254 2216 116-117 -1.5758659919082447 2216 118-119 -1.6764155526704094 2216 120-121 -1.7125255525225604 2216 122-123 -1.8835036199948476 2216 124-125 -2.25154739999698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23.0 3 17.0 4 37.0 5 181.0 6 571.0 7 1187.0 8 1964.0 9 3764.0 10 6937.0 11 9496.0 12 10949.0 13 15138.0 14 22832.0 15 23035.0 16 11958.0 17 10095.0 18 9766.0 19 9328.0 20 9321.0 21 9021.0 22 9310.0 23 10094.0 24 11480.0 25 13801.0 26 16269.0 27 20215.0 28 24997.0 29 31882.0 30 40340.0 31 52116.0 32 67426.0 33 90116.0 34 127014.0 35 198620.0 36 332459.0 37 602449.0 38 297940.0 39 2171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.25883505782164 19.05308530399014 12.836170517555894 22.851909120632325 2 13.92936520495724 22.620308632207028 37.98325062241098 25.467075540424755 3 21.236727253373076 27.38733609844928 29.975830580256158 21.400106067921488 4 13.163165850804939 18.513067648013443 35.12951220798748 33.19425429319414 5 11.758816035021306 39.048309690688534 34.817344708668216 14.375529565621942 6 31.02616134014494 33.274705952239515 18.30984911488654 17.389283592729 7 24.201226144895333 34.114552023718836 22.658684353465418 19.02553747792041 8 27.353837512278318 33.35825034132183 21.58954987337946 17.698362273020393 9 28.157612985483667 15.036598538529567 21.057358698942508 35.74842977704426 10-11 24.118117100619514 27.685091396573284 26.13955539525029 22.057236107556914 12-13 26.710073338757383 25.78445191730095 26.754222966550834 20.751251777390827 14-15 26.091110562496272 24.419524975056902 26.346921371797936 23.142443090648886 16-17 21.6451390088478 28.17550278393116 30.210042871565644 19.969315335655395 18-19 22.667166907678922 25.712071221140903 32.58303517670087 19.037726694479307 20-21 21.31482566552473 25.52383980170288 32.884090182230146 20.277244350542244 22-23 20.368299673195935 26.562726468753073 30.039932158574818 23.02904169947617 24-25 21.073064492598316 27.41694581477428 29.206360822669946 22.303628869957453 26-27 22.12485369376031 24.976251224267802 30.07502664757577 22.823868434396115 28-29 21.597094161973335 24.332282872981736 32.35714891612023 21.713474048924695 30-31 23.086803854358585 25.670846482876406 31.2585211652796 19.98382849748541 32-33 19.75613013046026 25.848077216429637 32.463115145564906 21.932677507545193 34-35 18.770732662379768 26.66366807115938 33.16073588670156 21.40486337975929 36-37 22.426020009323683 25.588397006347424 30.91375404584571 21.071828938483186 38-39 23.362736353185994 24.55637667102754 30.890183475033968 21.1907035007525 40-41 21.223165599694152 26.180257408752528 30.79790440468019 21.79867258687314 42-43 23.008274886079537 25.644876085802583 30.326153021476305 21.02069600664158 44-45 24.24929395210517 24.198398626824165 30.091326457633087 21.460980963437578 46-47 21.737554999978613 25.86670557078086 32.6452643349226 19.750475094317924 48-49 23.12717320900491 25.653002230175176 31.45143393183258 19.768390628987333 50-51 20.81949077112358 26.47600007413325 31.140882157125414 21.563626997617757 52-53 20.304312226425747 27.55741881340234 28.271165160795487 23.867103799376423 54-55 21.19578828114939 27.907033106672515 27.44410424465112 23.453074367526977 56-57 22.36177119533683 25.815572638939248 28.02395929514489 23.798696870579032 58-59 22.180073458444276 24.98093207351167 30.16089765857743 22.678096809466624 60-61 23.278671152044915 26.39050923362855 29.28522244013384 21.045597174192697 62-63 20.14014985370564 26.447225919644314 30.63047950429569 22.78214472235436 64-65 19.0451400191701 26.981199143285785 31.444044367797847 22.52961646974627 66-67 22.456528691705014 26.39775623372692 28.935655668175787 22.210059406392283 68-69 23.40179887174901 25.27663343818119 29.038586830228684 22.282980859841118 70-71 21.36056843092706 26.749532746698574 29.006842593732223 22.88305622864214 72-73 23.111918867814243 26.353014918365513 28.5953080307691 21.939758183051143 74-75 24.054385290443133 24.81653209423096 28.606998273550733 22.522084341775177 76-77 21.601240116161094 26.26778544507748 31.45563956795524 20.675334870806182 78-79 22.880466317131575 25.990356024918277 30.526241506159472 20.602936151790672 80-81 20.574518407142644 26.725391920141384 30.303128945753947 22.39696072696202 82-83 20.116294155021173 27.519092875177193 27.770361813014095 24.594251156787543 84-85 21.029439928071742 27.787493246033513 26.960598654481572 24.222468171413176 86-87 21.98846752797461 25.62501217733623 27.77615941309279 24.610360881596375 88-89 21.88893413973832 24.9863495030934 29.53554095172833 23.589175405439956 90-91 22.836152693579255 26.36297063325475 28.581621892878406 22.21925478028759 92-93 20.049669275428297 26.458535991928983 29.542882994450935 23.948911738191786 94-95 18.99496840282431 27.00470274703916 30.37646212378261 23.623866726353917 96-97 22.298931854995747 26.210125148775898 28.258521838246352 23.232421157982003 98-99 22.891682783001777 25.224696046989127 28.221918548410667 23.661702621598433 100-101 21.116498971876414 26.46131598868803 28.298775043137468 24.12340999629809 102-103 22.59211744726514 25.92982371932339 28.11779798733931 23.360260846072162 104-105 23.481096681919748 24.540399279982243 28.194033092867937 23.784470945230073 106-107 21.342663391877906 25.915461494496668 30.70729971327775 22.034575400347677 108-109 22.46665716051143 25.77948096392414 29.589357859213838 22.164504016350588 110-111 20.381194282323413 26.316008358498106 29.666132992136518 23.636664367041966 112-113 19.774528610374944 27.00309696995046 27.370874033741572 25.851500385933022 114-115 20.518913671431534 27.450162875121464 26.61786434869919 25.413059104747816 116-117 21.49714341404511 25.610722269960746 27.153694664418005 25.738439651576144 118-119 21.125790843481614 25.072325890850827 28.76511131639657 25.036771949270992 120-121 21.9850153800527 26.244710136386278 27.912205374083186 23.85806910947784 122-123 19.6525233496962 26.4042430188525 28.58056990675804 25.362663724693256 124-125 18.758693578762934 27.12282520934684 29.251816414427147 24.866664797463077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1586.0 1 1246.0 2 1601.0 3 2175.0 4 1396.0 5 902.0 6 1889.0 7 4499.0 8 7083.5 9 7548.5 10 5866.0 11 3858.5 12 3145.5 13 3149.0 14 3453.5 15 4012.0 16 4401.5 17 4715.5 18 5117.0 19 5214.0 20 5395.5 21 6015.5 22 6743.5 23 7741.0 24 9089.5 25 10645.5 26 12992.0 27 15465.0 28 16987.0 29 19018.5 30 22320.5 31 25475.5 32 28553.5 33 32275.5 34 36808.5 35 40769.0 36 44809.5 37 48529.0 38 49346.0 39 49388.5 40 49733.0 41 50341.0 42 51283.0 43 53250.0 44 55509.0 45 57227.5 46 59737.5 47 66195.0 48 76268.5 49 109955.0 50 226155.5 51 222543.5 52 95961.5 53 54953.0 54 46101.5 55 40671.5 56 35653.0 57 31203.5 58 28794.0 59 26310.0 60 22574.0 61 18741.5 62 15404.5 63 11684.0 64 8611.5 65 6862.5 66 5138.5 67 3800.0 68 2833.0 69 1970.0 70 1561.5 71 1405.0 72 1339.0 73 1279.5 74 997.5 75 774.5 76 510.5 77 241.5 78 143.5 79 75.5 80 43.5 81 24.5 82 10.0 83 5.5 84 3.5 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14807640856733223 2 9.504262424090645E-5 3 3.326491848431725E-4 4 0.0 5 0.0 6 0.003278970536311272 7 0.0 8 0.0 9 0.0 10-11 2.376065606022661E-5 12-13 0.005274865645370308 14-15 0.0014018787075533701 16-17 4.989737772647588E-4 18-19 0.0 20-21 9.504262424090645E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.6632459242158626E-4 30-31 0.0 32-33 0.0 34-35 2.376065606022661E-5 36-37 0.0 38-39 0.0 40-41 2.376065606022661E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.078623060477047E-4 96-97 0.0030176033196487794 98-99 0.004158114810539656 100-101 0.0 102-103 0.004253157434780564 104-105 0.022905272442058453 106-107 0.017416560892146105 108-109 0.019911429778469898 110-111 0.01675126252245976 112-113 0.015539469063388203 114-115 0.04120097760843294 116-117 0.04022679070996365 118-119 0.0225013412890346 120-121 0.0140187870755337 122-123 0.0029700820075283262 124-125 0.0017107672363363162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2104319.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.90448897512333 #Duplication Level Percentage of deduplicated Percentage of total 1 83.72827458268212 38.4350365748683 2 10.20133891389303 9.365744994085984 3 2.743948113042605 3.778786077104242 4 1.0256415240444996 1.8832620013171768 5 0.5587885449857116 1.2825451301360904 6 0.33588578353703435 0.925119914836587 7 0.2131844712138504 0.6850286945952583 8 0.14544742332959462 0.5341351712554777 9 0.12026867008662553 0.4968784656039822 >10 0.7658894852784466 6.9294097035630635 >50 0.0937490133887745 2.9745018808172223 >100 0.057388401130531126 4.916638294286056 >500 0.004604226691004188 1.4461943525764454 >1k 0.00213767667796623 2.051574524175333 >5k 0.0018088033428945024 5.642188489588786 >10k+ 0.0016443666753586386 18.65295573119007 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 58866 2.7973895592825992 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 50938 2.4206405967916464 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 32376 1.5385500012117934 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 18305 0.8698776183648962 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17143 0.8146578536809295 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16520 0.7850520762298872 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 14896 0.7078774653462712 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13879 0.6595482909197702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12970 0.6163514182022782 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11219 0.5331416006793647 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9390 0.4462251208110557 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8782 0.4173321630418202 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8239 0.39152809056041404 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7372 0.3503271129519811 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7161 0.3403001160945655 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6903 0.32803961756748856 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6606 0.31392578786771397 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5145 0.2444971508597318 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5105 0.2425962983749137 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5042 0.23960245571132516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5002 0.237701603226507 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3897 0.1851905533334062 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3221 0.1530661463399798 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3216 0.15282853977937758 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3015 0.14327675604316645 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2373 0.11276807366183549 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.504262424090645E-5 0.0 0.0 0.0 0.0 6 1.4256393636135966E-4 0.0 0.0 0.0 0.0 7 2.376065606022661E-4 0.0 0.0 0.0 0.0 8 2.376065606022661E-4 0.0 0.0 0.0 0.0 9 2.376065606022661E-4 0.0 0.0 0.0 0.0 10-11 2.376065606022661E-4 0.0 0.0 1.4256393636135966E-4 0.0 12-13 2.376065606022661E-4 0.0 0.0 5.227344333249854E-4 0.0 14-15 2.376065606022661E-4 0.0 0.0 0.001164272146951104 0.0 16-17 2.851278727227193E-4 0.0 0.0 0.0020196557651192618 0.0 18-19 3.5640984090339913E-4 0.0 0.0 0.002851278727227193 0.0 20-21 3.801704969636258E-4 0.0 0.0 0.004253157434780564 0.0 22-23 3.801704969636258E-4 0.0 0.0 0.007151957474128209 0.0 24-25 4.27691809084079E-4 0.0 0.0 0.009931954233174722 0.0 26-27 4.27691809084079E-4 0.0 0.0 0.012664429680100783 0.0 28-29 6.890590257465717E-4 0.0 0.0 0.016988869083062026 0.0 30-31 7.603409939272516E-4 0.0 0.0 0.024782364270816357 0.0 32-33 7.841016499874782E-4 0.0 0.0 0.0431255907493113 0.0 34-35 9.029049302886113E-4 0.0 0.0 0.07002265340948782 0.0 36-37 0.0010454688666499709 0.0 0.0 0.1126017490694139 0.0 38-39 0.0010454688666499709 0.0 0.0 0.17856133029260296 0.0 40-41 0.0010692295227101975 0.0 0.0 0.26452738391850283 0.0 42-43 0.0011880328030113305 0.0 0.0 0.3360707193158452 0.0 44-45 0.0014256393636135965 0.0 0.0 0.4169044712327361 0.0 46-47 0.0016870065802760893 0.0 0.0 0.5201207611583605 0.0 48-49 0.001710767236336316 0.0 0.0 0.6207471395734202 0.0 50-51 0.001710767236336316 0.0 0.0 0.722965481944515 0.0 52-53 0.0017345278923965425 0.0 0.0 0.8365889392245187 0.0 54-55 0.0017582885484567691 0.0 0.0 0.964967763917923 0.0 56-57 0.0018058098605772223 0.0 0.0 1.1178675856654814 0.0 58-59 0.0018058098605772223 0.0 0.0 1.2931024241096525 0.0 60-61 0.0018058098605772223 0.0 0.0 1.46163675754484 0.0 62-63 0.0018058098605772223 0.0 0.0 1.6307413467254728 0.0 64-65 0.0018533311726976755 0.0 0.0 1.8183792476330822 0.0 66-67 0.0019958951090590354 0.0 0.0 2.0402800145795386 0.0 68-69 0.0020909377332999418 0.0 0.0 2.2963010836284803 0.0 70-71 0.002162219701480622 0.0 0.0 2.641448373559332 0.0 72-73 0.002185980357540848 0.0 0.0 2.9271465020274965 0.0 74-75 0.002185980357540848 0.0 0.0 3.2120130075335536 0.0 76-77 0.002185980357540848 0.0 0.0 3.553263549870528 0.0 78-79 0.002185980357540848 0.0 0.0 3.906370659581556 0.0 80-81 0.0027087147908658334 0.0 0.0 4.276751766248369 0.0 82-83 0.0027087147908658334 0.0 0.0 4.697291617858319 0.0 84-85 0.0027324754469260602 0.0 0.0 5.102054393844279 0.0 86-87 0.0027562361029862866 0.0 0.0 5.5288195373420095 0.0 88-89 0.0027562361029862866 0.0 0.0 6.014653671805463 0.0 90-91 0.0027562361029862866 0.0 0.0 6.4975890062295685 0.0 92-93 0.002993842663588553 0.0 0.0 6.998059704826122 0.0 94-95 0.002993842663588553 0.0 0.0 7.531795321906992 0.0 96-97 0.002993842663588553 0.0 0.0 8.067835722625704 0.0 98-99 0.003112645943889686 0.0 0.0 8.688606622855186 0.0 100-101 0.0032076885681305923 0.0 0.0 9.481618518865249 0.0 102-103 0.0032789705363112723 0.0 0.0 10.184957698904016 0.0 104-105 0.0033264918484317255 0.0 0.0 10.852418288291842 0.0 106-107 0.0033502525044919523 0.0 0.0 11.544589959982304 0.0 108-109 0.003397773816612405 0.0 0.0 12.213000975612538 0.0 110-111 0.003421534472672632 0.0 0.0 12.932711247676803 0.0 112-113 0.003421534472672632 0.0 0.0 13.752905334219765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCA 1425 0.0 39.30344 1 AGTACTC 1700 0.0 37.097496 5 AAAGTAC 1965 0.0 30.883411 3 AAGTACT 2190 0.0 29.340487 4 TTATCAA 1970 0.0 27.482918 2 GCAACGC 170 1.4002135E-5 24.498346 4 CATGGGG 5090 0.0 22.325607 4 GAGTACT 16520 0.0 22.132763 12-13 GTACTTT 18015 0.0 20.410675 14-15 AGTACTT 16735 0.0 20.10623 12-13 GTGTTAT 815 0.0 19.73894 1 CTATACA 920 0.0 18.754168 4 AAAAAGT 3270 0.0 18.585318 1 ATGGGAT 2670 0.0 18.495005 5 CATGGGT 4120 0.0 18.48419 4 CATATAG 550 0.0 18.38967 3 ACTTTTT 20550 0.0 18.08041 16-17 AAAAGTA 3325 0.0 18.072464 2 TATACAG 965 0.0 17.879622 5 CGCGTCA 85 0.009699626 17.498402 26-27 >>END_MODULE