##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2102322 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.70748819638476 31.0 31.0 34.0 27.0 34.0 2 30.80350964314696 31.0 31.0 34.0 27.0 34.0 3 30.91407025184534 33.0 31.0 34.0 27.0 34.0 4 34.26728303276092 37.0 35.0 37.0 30.0 37.0 5 34.3118637392369 37.0 35.0 37.0 31.0 37.0 6 34.26798796759012 37.0 35.0 37.0 30.0 37.0 7 34.20937277924124 37.0 35.0 37.0 30.0 37.0 8 34.12438912783104 37.0 35.0 37.0 30.0 37.0 9 35.532229601364584 39.0 37.0 39.0 29.0 39.0 10-11 35.46326252591183 39.0 37.0 39.0 28.5 39.0 12-13 35.36603574523788 39.0 37.0 39.0 27.5 39.0 14-15 36.58225975849561 40.0 37.0 41.0 27.0 41.0 16-17 36.62355742840535 40.0 37.0 41.0 28.0 41.0 18-19 36.5966245893826 40.0 37.0 41.0 28.0 41.0 20-21 36.62717794895359 40.0 37.0 41.0 28.5 41.0 22-23 36.63374211942795 40.0 37.0 41.0 28.5 41.0 24-25 36.633514989616245 40.0 37.0 41.0 29.0 41.0 26-27 36.48773261184538 40.0 37.0 41.0 28.0 41.0 28-29 36.405153206787546 40.0 36.0 41.0 28.5 41.0 30-31 36.477703225290895 40.0 36.0 41.0 29.5 41.0 32-33 36.206107104430245 40.0 36.0 41.0 29.0 41.0 34-35 36.2748163697093 40.0 36.0 41.0 29.5 41.0 36-37 36.28114722673311 40.0 36.0 41.0 29.5 41.0 38-39 36.16537166999156 40.0 36.0 41.0 29.0 41.0 40-41 35.88218384243707 40.0 35.0 41.0 28.0 41.0 42-43 35.66540496650846 40.0 35.0 41.0 27.0 41.0 44-45 35.46204767870954 39.0 35.0 41.0 27.0 41.0 46-47 35.2325419226931 39.0 35.0 41.0 26.0 41.0 48-49 35.00112137912271 39.0 34.0 41.0 24.5 41.0 50-51 34.89570627144653 39.0 34.0 41.0 24.5 41.0 52-53 34.7554856011591 39.0 34.0 41.0 24.5 41.0 54-55 34.49839154040152 38.0 33.5 40.5 24.0 41.0 56-57 34.157530578094125 38.0 33.0 40.0 23.0 41.0 58-59 33.824733080850606 38.0 33.0 40.0 22.0 41.0 60-61 33.56178882207388 37.5 32.0 40.0 21.5 41.0 62-63 33.04118969406209 37.0 31.5 40.0 19.0 41.0 64-65 32.802203230523205 36.5 32.0 40.0 18.0 41.0 66-67 32.411666005492975 36.0 31.0 39.0 15.5 41.0 68-69 32.0145403510975 35.0 31.0 39.0 10.5 41.0 70-71 31.506437643710143 35.0 30.5 39.0 7.5 40.5 72-73 31.277270323004757 35.0 30.0 37.5 4.5 40.0 74-75 30.955003324895046 35.0 30.0 37.0 2.0 39.5 76-77 30.445585642922445 35.0 30.0 36.5 2.0 39.0 78-79 30.08643870919869 35.0 30.0 36.0 2.0 39.0 80-81 29.840799363751128 35.0 30.0 36.0 2.0 37.0 82-83 29.595545068738282 35.0 29.5 35.5 2.0 37.0 84-85 29.311354778192875 34.0 29.0 35.0 2.0 36.5 86-87 28.96286439470262 34.0 29.0 35.0 2.0 36.0 88-89 28.649036636633205 34.0 29.0 35.0 2.0 36.0 90-91 28.49803931081918 34.0 29.0 35.0 2.0 36.0 92-93 28.171629750342717 34.0 28.0 35.0 2.0 35.5 94-95 28.095762923091705 34.0 28.0 35.0 2.0 35.0 96-97 28.021812310388228 34.0 28.0 35.0 2.0 35.0 98-99 27.814748168929405 34.0 27.0 35.0 2.0 35.0 100-101 27.39534880955439 34.0 26.0 35.0 2.0 35.0 102-103 26.215670101915883 32.5 24.0 34.5 2.0 35.0 104-105 26.985893930615767 34.0 25.0 35.0 2.0 35.0 106-107 27.017426921280375 34.0 25.0 35.0 2.0 35.0 108-109 26.982506247853564 34.0 25.0 35.0 2.0 35.0 110-111 26.976484810604653 34.0 25.0 35.0 2.0 35.0 112-113 26.878832072346672 34.0 25.0 35.0 2.0 35.0 114-115 26.702895655375343 34.0 25.0 35.0 2.0 35.0 116-117 26.38783949366462 34.0 24.0 35.0 2.0 35.0 118-119 26.137084851892336 34.0 24.0 35.0 2.0 35.0 120-121 25.95420016534099 34.0 23.0 35.0 2.0 35.0 122-123 25.49941612179295 34.0 18.5 35.0 2.0 35.0 124-125 24.514560091175376 32.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39653678177886675 1101 2 0.44159815197023633 1101 3 0.43019437487507517 1101 4 0.6045224651208301 1101 5 0.46233783968721553 1101 6 0.5032735301439288 1101 7 0.5153956884172572 1101 8 0.457786984013417 1101 9 0.6010528423234902 1101 10-11 0.577445135318726 1101 12-13 0.6442654525462572 1101 14-15 0.5547870327799274 1101 16-17 0.6834543412545955 1101 18-19 0.6874950914822904 1101 20-21 0.6521934566294831 1101 22-23 0.6298320084289699 1101 24-25 0.7038826434161933 1101 26-27 0.8928518018934071 1101 28-29 0.8521347478329204 1101 30-31 0.7963960466051958 1101 32-33 0.8564863168322603 1101 34-35 0.8378893650043722 1101 36-37 0.7696741222856076 1101 38-39 0.7026735015483254 1101 40-41 0.8291278423391901 1101 42-43 0.9085247022301175 1101 44-45 0.8588961167285802 1101 46-47 0.8374776515372346 1101 48-49 0.8385371061003895 1101 50-51 0.7525838480401532 1101 52-53 0.7699035783754411 1101 54-55 0.7801205164959271 1101 56-57 0.8330478174494971 1101 58-59 0.8697494139588784 1101 60-61 0.8185398554906556 1101 62-63 0.7919247155280331 1101 64-65 0.8524315734561725 1101 66-67 0.8668052717586789 1101 68-69 0.8436275009723602 1101 70-71 0.7988079610278724 1101 72-73 0.7447019032199584 1101 74-75 0.6998708793170039 1101 76-77 0.683871015941488 1101 78-79 0.7311236089133111 1101 80-81 0.5928331749412266 1101 82-83 0.5579022877727624 1101 84-85 0.647624835343386 1101 86-87 0.6301458882577293 1101 88-89 0.639260822479347 1101 90-91 0.6120627448723539 1101 92-93 0.6407034813673889 1101 94-95 0.6099244555479331 1101 96-97 0.595307187710592 1101 98-99 0.545312920817171 1101 100-101 0.6202537730814406 1101 102-103 0.646094119934979 1101 104-105 0.6690055776080044 1101 106-107 0.5565141049791933 1101 108-109 0.5636209006657076 1101 110-111 0.47484659667203033 1101 112-113 0.5595097451071709 1101 114-115 0.5090272919211252 1101 116-117 0.5289420100337452 1101 118-119 0.5665699877472541 1101 120-121 0.4351934607789083 1101 122-123 0.33789115831960714 1101 124-125 0.5020059405046382 1102 1 0.34781038740029757 1102 2 0.36499046014515457 1102 3 0.3440597365665212 1102 4 0.45614531891759924 1102 5 0.3823673271080068 1102 6 0.44132798776360715 1102 7 0.46288612069029966 1102 8 0.4716988772731341 1102 9 0.5412775532003735 1102 10-11 0.531955821894627 1102 12-13 0.5778037339106277 1102 14-15 0.5561236390078363 1102 16-17 0.5310770486599097 1102 18-19 0.564859836238341 1102 20-21 0.6090613069755975 1102 22-23 0.49582475159562733 1102 24-25 0.6074972051829306 1102 26-27 0.70355249411584 1102 28-29 0.6813471618279436 1102 30-31 0.5542666783888706 1102 32-33 0.6142534289471939 1102 34-35 0.5033211152187249 1102 36-37 0.6047790535861992 1102 38-39 0.5882623088762671 1102 40-41 0.6263166036583812 1102 42-43 0.6210500593206945 1102 44-45 0.5688862875883203 1102 46-47 0.6141130531726304 1102 48-49 0.6714017253780469 1102 50-51 0.6390559709253196 1102 52-53 0.5902820525582655 1102 54-55 0.6025631101341702 1102 56-57 0.6419154795780244 1102 58-59 0.5492441543231834 1102 60-61 0.5388067061922897 1102 62-63 0.5368522517599175 1102 64-65 0.635926721625232 1102 66-67 0.6899798226506206 1102 68-69 0.6900960686365778 1102 70-71 0.6496000433416889 1102 72-73 0.47683691796367 1102 74-75 0.47405631545895943 1102 76-77 0.5553905592001591 1102 78-79 0.460884990848605 1102 80-81 0.5082340783856054 1102 82-83 0.46796460870464784 1102 84-85 0.4014025190123718 1102 86-87 0.38120356648931875 1102 88-89 0.3982442907019461 1102 90-91 0.4749896327992431 1102 92-93 0.32346745652266407 1102 94-95 0.4702225712808321 1102 96-97 0.5248683270543388 1102 98-99 0.48520798951659216 1102 100-101 0.4149653819234871 1102 102-103 0.26448559158732365 1102 104-105 0.4304379656708264 1102 106-107 0.4798128271909583 1102 108-109 0.39008481517310045 1102 110-111 0.5025216390209835 1102 112-113 0.6413707987073565 1102 114-115 0.59686582215096 1102 116-117 0.5593475143522966 1102 118-119 0.5679766527408745 1102 120-121 0.571550038983311 1102 122-123 0.47410578909945755 1102 124-125 0.46357818468558065 1103 1 0.3201334194666998 1103 2 0.31480123134810967 1103 3 0.34106267060720796 1103 4 0.4269232119357653 1103 5 0.33207744144075235 1103 6 0.41319638120040736 1103 7 0.348387554056373 1103 8 0.35667642579392833 1103 9 0.5132253911173166 1103 10-11 0.5734862142524548 1103 12-13 0.5944698396727475 1103 14-15 0.6407175288196711 1103 16-17 0.7303225300638871 1103 18-19 0.6416112096896001 1103 20-21 0.6174287280078872 1103 22-23 0.6644270998480053 1103 24-25 0.6611329374540134 1103 26-27 0.77626662962426 1103 28-29 0.8820961733611625 1103 30-31 0.7001539732890265 1103 32-33 0.7321116098419722 1103 34-35 0.6108395307347791 1103 36-37 0.5909668811417035 1103 38-39 0.7514290841544522 1103 40-41 0.8402126568280579 1103 42-43 0.8592215512961943 1103 44-45 0.7261921202817163 1103 46-47 0.7117439963387255 1103 48-49 0.6634070509117507 1103 50-51 0.7712308833987223 1103 52-53 0.7058182416096059 1103 54-55 0.7164900130538356 1103 56-57 0.7508712384869796 1103 58-59 0.7593166231904362 1103 60-61 0.7325541502522555 1103 62-63 0.7016143925388008 1103 64-65 0.6922439855532545 1103 66-67 0.7915506103339709 1103 68-69 0.8148526105682734 1103 70-71 0.7674822411492634 1103 72-73 0.765484413932807 1103 74-75 0.7850256706940897 1103 76-77 0.7672062684027701 1103 78-79 0.7596658393238123 1103 80-81 0.7725538184522414 1103 82-83 0.8176108819018744 1103 84-85 0.7679341096662746 1103 86-87 0.7434094612901418 1103 88-89 0.8540892730028595 1103 90-91 0.810520794106214 1103 92-93 0.6609264977430342 1103 94-95 0.6841562560463608 1103 96-97 0.7645144370793382 1103 98-99 0.814441314589331 1103 100-101 0.6995977191923295 1103 102-103 0.5926201556472108 1103 104-105 0.7173502269422016 1103 106-107 0.6775238267330579 1103 108-109 0.6951065935668517 1103 110-111 0.6981793199968358 1103 112-113 0.5995848378421798 1103 114-115 0.6177684399434646 1103 116-117 0.5917464317045393 1103 118-119 0.4940136887580877 1103 120-121 0.3232200557798528 1103 122-123 0.18120745253837 1103 124-125 0.23437683117192165 1104 1 0.26283948940634616 1104 2 0.3667349162394764 1104 3 0.39740226876633855 1104 4 0.39918143909392967 1104 5 0.34057330606862735 1104 6 0.32039194258022263 1104 7 0.3985554819591357 1104 8 0.36275656031872217 1104 9 0.5369118236177925 1104 10-11 0.5235321011246015 1104 12-13 0.46006136975150724 1104 14-15 0.6243387815322734 1104 16-17 0.530534093635012 1104 18-19 0.5391403300520494 1104 20-21 0.4977673784796366 1104 22-23 0.4841764347511628 1104 24-25 0.48944760695250267 1104 26-27 0.5749814401849562 1104 28-29 0.5453713734059136 1104 30-31 0.5754224070283556 1104 32-33 0.5427808084352392 1104 34-35 0.4471622875093644 1104 36-37 0.4973420423705335 1104 38-39 0.5192800393888177 1104 40-41 0.6207299061069236 1104 42-43 0.5519473068725844 1104 44-45 0.5820868627192013 1104 46-47 0.4287608359381707 1104 48-49 0.5239432400541233 1104 50-51 0.5499160296387018 1104 52-53 0.5083012400623304 1104 54-55 0.6346187390688414 1104 56-57 0.6390716479878904 1104 58-59 0.6453889013114917 1104 60-61 0.6209066399745709 1104 62-63 0.6279096826436046 1104 64-65 0.6617898592355331 1104 66-67 0.5283729360079903 1104 68-69 0.5853524492154705 1104 70-71 0.5528845530859847 1104 72-73 0.4625916864714732 1104 74-75 0.5532072157021979 1104 76-77 0.5324360587909034 1104 78-79 0.5176594970777622 1104 80-81 0.586790431277123 1104 82-83 0.6081686984682335 1104 84-85 0.5287423672008167 1104 86-87 0.5195395903902309 1104 88-89 0.4738143405240969 1104 90-91 0.5114281383263801 1104 92-93 0.4596008461385388 1104 94-95 0.5725426625836185 1104 96-97 0.40237225699771173 1104 98-99 0.4373365134162448 1104 100-101 0.4613372511442506 1104 102-103 0.24248527184602153 1104 104-105 0.4510347017879255 1104 106-107 0.5058496034925781 1104 108-109 0.46871656447574495 1104 110-111 0.623511848582659 1104 112-113 0.6801821982412619 1104 114-115 0.6463397367578452 1104 116-117 0.5595117221881765 1104 118-119 0.5376886787962611 1104 120-121 0.4837465558204137 1104 122-123 0.40188046528573906 1104 124-125 0.5038167197660925 1105 1 0.17308386177422008 1105 2 0.24706331260781056 1105 3 0.1946733186643641 1105 4 0.17500810974440384 1105 5 0.28314193980165925 1105 6 0.2774404870695051 1105 7 0.23135744058591 1105 8 0.22347299021666345 1105 9 0.30042298048672933 1105 10-11 0.307276494891461 1105 12-13 0.3674977757785811 1105 14-15 0.4321536795431271 1105 16-17 0.356668419641089 1105 18-19 0.35104313347861194 1105 20-21 0.40777306220909537 1105 22-23 0.37701193578131864 1105 24-25 0.514599209736204 1105 26-27 0.5103643659576989 1105 28-29 0.5425068597567559 1105 30-31 0.452114976462056 1105 32-33 0.369890887995453 1105 34-35 0.4323926905421729 1105 36-37 0.4793681479279144 1105 38-39 0.4555939208480595 1105 40-41 0.5309170811973942 1105 42-43 0.539122967311144 1105 44-45 0.5912653758219832 1105 46-47 0.5841641387409524 1105 48-49 0.47496522140781394 1105 50-51 0.46270248100361755 1105 52-53 0.4633621791616278 1105 54-55 0.5020883475154818 1105 56-57 0.4859823350391892 1105 58-59 0.4563295005389634 1105 60-61 0.4382625338340276 1105 62-63 0.5341660948383833 1105 64-65 0.5885698445082355 1105 66-67 0.5418469446451795 1105 68-69 0.4489124937573479 1105 70-71 0.4574016980698836 1105 72-73 0.38484263732621216 1105 74-75 0.470074972796958 1105 76-77 0.4205510345180592 1105 78-79 0.4311845632846101 1105 80-81 0.35822436102206723 1105 82-83 0.29500525504814235 1105 84-85 0.2841056119103662 1105 86-87 0.24981092947449213 1105 88-89 0.21800246716927063 1105 90-91 0.22939576306744414 1105 92-93 0.19170694788809328 1105 94-95 0.2863131371090333 1105 96-97 0.2842400300917127 1105 98-99 0.23303268827831758 1105 100-101 0.14373571897717952 1105 102-103 0.05363546109700934 1105 104-105 0.07820574844610562 1105 106-107 0.09273136792059589 1105 108-109 0.06015811185809028 1105 110-111 0.12709962780436967 1105 112-113 0.03552233405079619 1105 114-115 0.14326623305662167 1105 116-117 0.08925200655064103 1105 118-119 0.05882567267535421 1105 120-121 0.08459490543897275 1105 122-123 0.14859632161097736 1105 124-125 0.279853380421045 1106 1 0.1233523760517663 1106 2 0.15427260591603797 1106 3 0.1518927903116314 1106 4 0.13883865493986747 1106 5 0.06492743857662475 1106 6 0.11689839357904219 1106 7 0.0810318549073159 1106 8 0.13747208385980514 1106 9 0.30753751830531684 1106 10-11 0.2542758236057665 1106 12-13 0.19218679116968218 1106 14-15 0.2162647470327883 1106 16-17 0.24023761174754554 1106 18-19 0.23752466342837408 1106 20-21 0.19958907176703633 1106 22-23 0.17140585204266046 1106 24-25 0.3292565026372998 1106 26-27 0.426539382497495 1106 28-29 0.4755664078869728 1106 30-31 0.3299942952888344 1106 32-33 0.02338175535957987 1106 34-35 0.1912363342906218 1106 36-37 0.2959747745113148 1106 38-39 0.1383947695276646 1106 40-41 0.26126864587467935 1106 42-43 0.24954407185480676 1106 44-45 0.0943179687538489 1106 46-47 0.14641679904374172 1106 48-49 -0.01650829915848817 1106 50-51 0.08294016945435345 1106 52-53 0.040410388137473774 1106 54-55 0.05248748381071522 1106 56-57 0.10608128303514519 1106 58-59 0.07872039237957296 1106 60-61 0.057055261653118805 1106 62-63 0.03376532273865962 1106 64-65 -0.01069683039803948 1106 66-67 0.027298281279200864 1106 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-1.9744876670812808 2216 92-93 -1.9794194064974349 2216 94-95 -1.943200108060502 2216 96-97 -2.061236940204502 2216 98-99 -2.1048808370378715 2216 100-101 -2.0458386022851265 2216 102-103 -1.8898732728872503 2216 104-105 -1.9618176314410114 2216 106-107 -2.0693813705130566 2216 108-109 -2.078754465903206 2216 110-111 -2.1224023908995377 2216 112-113 -2.0618420214058837 2216 114-115 -2.092878866436646 2216 116-117 -2.0994792998391745 2216 118-119 -2.1277813598299 2216 120-121 -2.1472496337609606 2216 122-123 -2.1714022364576557 2216 124-125 -2.239807353728011 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51802.0 3 8888.0 4 6076.0 5 4344.0 6 5067.0 7 5288.0 8 6134.0 9 7107.0 10 8745.0 11 11004.0 12 12628.0 13 13794.0 14 14377.0 15 14356.0 16 14090.0 17 14180.0 18 14382.0 19 14649.0 20 14975.0 21 16357.0 22 18224.0 23 21261.0 24 25237.0 25 28131.0 26 31707.0 27 34318.0 28 39754.0 29 48991.0 30 58461.0 31 69960.0 32 88532.0 33 111183.0 34 137860.0 35 176591.0 36 262713.0 37 456636.0 38 232934.0 39 1586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.338017208041 17.70738873068828 10.936461036679543 23.01813302459118 2 13.255472708154095 22.7300449287831 38.26001338304177 25.754468980021027 3 22.229560403640335 30.189586324093597 26.588344589105382 20.99250868316069 4 13.120996461689327 18.65397959593295 33.82336355520892 34.401660387168796 5 11.970442007829178 41.35891827153992 32.850242469398005 13.820397251232897 6 31.00053289745667 30.796311298142985 20.757861432323217 17.44529437207713 7 26.372405475095047 33.592730073838474 21.685296720562572 18.34956773050391 8 28.611743921593746 32.956419406924596 21.77743850850685 16.654398162974804 9 31.072563951792947 16.286660834397004 19.20858012917043 33.43219508463962 10-11 23.951696026986507 29.583900651714956 24.7481488847413 21.716254436557232 12-13 26.855178239720956 26.84485180732885 24.627035317115894 21.6729346358343 14-15 27.210534667396978 25.79232731781132 23.343604552793305 23.6535334619984 16-17 23.047754470446954 29.268155122866347 28.399457512083497 19.284632894603202 18-19 24.55987676047504 28.131089425835615 28.56702511170195 18.742008701987398 20-21 22.55420087067426 28.572210354557082 28.244339386599304 20.629249388169356 22-23 21.08261039162184 30.111151690603254 23.79290086217611 25.01333705559879 24-25 21.769779492720218 31.202488673995322 22.889773604177616 24.137958229106847 26-27 23.702806626073507 27.41914589923986 23.84453851809149 25.033508956595146 28-29 23.36566734518141 27.244553777969614 26.52478801492971 22.864990861919264 30-31 25.38097443980252 28.91559785391291 25.15667076360295 20.546756942681615 32-33 20.25719798943663 29.41599821975891 27.34711331086361 22.979690479940857 34-35 18.763756494188602 30.45887685103944 28.034746254301872 22.742620400470084 36-37 23.992854919362216 28.53493117353195 25.668107325284407 21.804106581821422 38-39 26.03445669485399 26.95496881912946 24.968339111460416 22.04223537455613 40-41 22.11409441649312 30.10642995082907 24.98514078464776 22.794334848030047 42-43 24.689560106264587 28.556816303838 24.653426899154677 22.100196690742735 44-45 26.389924780344558 26.853794452484713 24.62150079051591 22.134779976654816 46-47 22.8126903108214 28.33645555569625 28.981789173179294 19.86906496030306 48-49 24.380855860860077 27.872900240034003 28.186064935564474 19.560178963541446 50-51 21.173037522361753 29.00488906969128 28.23384596862558 21.588227439321383 52-53 20.216575541080562 30.312287264154325 24.174913353063275 25.29622384170184 54-55 21.268234865279783 30.94796815510734 23.11855221302608 24.665244766586795 56-57 23.368385756731836 27.5197705746405 23.826190419895887 25.285653248731776 58-59 23.19335975984468 27.119003132824147 26.400056174448412 23.287580932882758 60-61 24.842913165961946 28.868363311165457 25.243769434315418 21.04495408855718 62-63 20.062209066797116 29.39977948314212 27.155413746187772 23.382597703872996 64-65 18.459359723300246 30.127434686229442 27.96682871032313 23.44637688014718 66-67 23.38985917269332 28.71373618026354 25.55548867237849 22.34091597466465 68-69 25.342287907145067 27.05732846190504 24.97824458981228 22.62213904113761 70-71 21.64888704353065 29.826717357744304 24.990240996034764 23.534154602690283 72-73 24.315728491653477 28.40892286558963 24.76813091647505 22.50721772628184 74-75 25.75978241964492 26.757423897524696 24.707167690158236 22.775625992672143 76-77 22.398181397739933 28.200972176302745 28.90994230390686 20.49090412205046 78-79 23.984739290236543 27.86844756053895 27.958213631305096 20.188599517919414 80-81 20.853934334316833 29.08057484765591 27.961605156897274 22.103885661129986 82-83 20.031260025555536 30.086066594890752 24.187199920118047 25.695473459435664 84-85 21.079592158001866 30.62316944438539 23.29440171572076 25.00283668189198 86-87 22.899493762391067 27.256754035316472 24.2187311498696 25.625021052422863 88-89 22.972824490205397 26.802380992191548 26.45641097912571 23.768383538477337 90-91 24.345742086296738 28.59214216885083 25.360708359987445 21.701407384864986 92-93 20.13594501806875 29.001659596598817 27.046885574792096 23.815509810540338 94-95 18.69832252762741 29.60576293698609 27.91364672269389 23.782267812692602 96-97 23.34647541275276 28.08495567443848 25.795181778863917 22.773387133944848 98-99 24.91187582489576 26.79648210012014 25.130956678527976 23.160685396456124 100-101 21.56802166753494 28.984386825856067 25.17747886656101 24.27011264004798 102-103 23.857492391239482 27.98030673748284 25.052861490720296 23.10933938055738 104-105 25.115961856182235 26.453100945717843 25.089941903891845 23.340995294208078 106-107 22.144317668485378 27.85630310606936 28.75976884265657 21.23961038278869 108-109 23.456284567274963 27.944290739151995 27.691068996895673 20.908355696677365 110-111 20.544324096484807 29.01641712807127 27.891971022556984 22.547287752886938 112-113 19.901809144659214 29.85756140988103 24.352922549867355 25.887706895592395 114-115 20.555540696858053 30.964878178315196 23.471270867581303 25.008310257245448 116-117 22.32888207691876 27.927103961725642 24.138295726244746 25.605718235110853 118-119 22.21460891029854 27.544102458613967 26.12781142049349 24.113477210594002 120-121 23.470981220233273 29.204828523111665 25.184651061220958 22.1395391954341 122-123 20.081369098608924 29.666286870969845 26.308187610317674 23.944156420103553 124-125 19.004522617745312 30.39553025239402 26.926040788333417 23.67390634152725 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10056.0 1 5861.0 2 2336.0 3 3117.5 4 2940.0 5 2434.0 6 1984.0 7 1680.0 8 1606.5 9 1506.0 10 1335.0 11 1235.5 12 1204.5 13 1192.5 14 1258.5 15 1338.0 16 1426.5 17 1575.5 18 1828.5 19 2173.5 20 2578.5 21 3183.5 22 3911.5 23 4869.5 24 6275.5 25 7987.5 26 10191.0 27 12577.5 28 14529.5 29 16779.0 30 20099.5 31 23502.0 32 26769.0 33 30453.5 34 34664.5 35 37728.5 36 40481.0 37 43711.5 38 44559.5 39 44386.0 40 44393.5 41 45507.0 42 46865.0 43 48536.0 44 50466.5 45 51926.0 46 54903.5 47 62425.0 48 76143.5 49 117080.0 50 256348.5 51 268140.0 52 124599.0 53 64918.5 54 49595.0 55 42153.0 56 36122.5 57 31065.0 58 27570.5 59 24454.0 60 21005.0 61 17618.5 62 15236.0 63 12250.0 64 9464.0 65 7486.0 66 5459.5 67 4203.0 68 3142.0 69 2129.0 70 1684.5 71 1468.0 72 1379.0 73 1274.5 74 924.0 75 704.5 76 491.5 77 280.5 78 182.0 79 123.0 80 80.0 81 46.5 82 29.0 83 20.5 84 20.5 85 15.5 86 12.0 87 13.0 88 10.5 89 9.0 90 9.0 91 9.0 92 6.0 93 4.0 94 4.0 95 2.5 96 1.5 97 4.5 98 4.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4943581430437392 2 0.4814676343585807 3 0.4645339771928373 4 0.4660561036796456 5 0.47057491668735807 6 0.4750461632423577 7 0.4588260028673058 8 0.48727074158953765 9 0.5000185509165579 10-11 0.5046800632824087 12-13 0.504323314887063 14-15 0.4788990459120915 16-17 0.48087305369967115 18-19 0.4666982507912679 20-21 0.47735313619892666 22-23 0.4949765069290052 24-25 0.48153898403764983 26-27 0.4920273868608139 28-29 0.4876750564375962 30-31 0.5001612502746963 32-33 0.49804454312897833 34-35 0.49257440106701067 36-37 0.4477668026115885 38-39 0.41147359919175086 40-41 0.40448133064297476 42-43 0.4128768095467773 44-45 0.44641115870927484 46-47 0.45323694467355624 48-49 0.45157211882860954 50-51 0.4368265184876532 52-53 0.43080936221948873 54-55 0.43349686679775984 56-57 0.4158972792940378 58-59 0.420653924565314 60-61 0.4306190964086377 62-63 0.4308569286722015 64-65 0.44557874578680146 66-67 0.43380604874039275 68-69 0.4359227558861107 70-71 0.4463160258038492 72-73 0.44638737548291835 74-75 0.45121537043326376 76-77 0.4417020798907113 78-79 0.4330212022706322 80-81 0.44144046440079115 82-83 0.43920484112329133 84-85 0.43796811335275954 86-87 0.44391391994185475 88-89 0.431760691273744 90-91 0.4351854758690629 92-93 0.44443715092169517 94-95 0.4419874786069879 96-97 0.4121870959824423 98-99 0.3861444631222049 100-101 0.38666769410204527 102-103 0.3661189865301319 104-105 0.36985295306808375 106-107 0.3883325199469919 108-109 0.3951583059112734 110-111 0.41023687142121895 112-113 0.40633642229877254 114-115 0.4054564429235864 116-117 0.37908084489435967 118-119 0.4517148181867478 120-121 0.4562098479681038 122-123 0.41156873209717637 124-125 0.4049094287173896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2102322.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.40760521954179 #Duplication Level Percentage of deduplicated Percentage of total 1 85.05954947355944 42.87648190015268 2 9.813601651592583 9.893603156706444 3 2.5207852817614276 3.812002479787844 4 0.9140166007360973 1.8429355189605097 5 0.4780919725203444 1.2049735704718771 6 0.26774867229383814 0.8097941622626555 7 0.18047114661332508 0.6367982818401776 8 0.11952490930046326 0.48199715535354337 9 0.0893482460369279 0.4053448001959156 >10 0.4909303829441122 4.633817597616405 >50 0.03917767732708594 1.3792452745808594 >100 0.02055972005214298 1.7989580583253242 >500 0.0018451002908508123 0.6356370063645527 >1k 0.0017133074129328974 2.0939216977011217 >5k 0.0011861359012612365 4.533896192868063 >10k+ 0.0014497216570970668 22.960593146812037 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 90226 4.291730762461697 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 66427 3.1596967543506653 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 44301 2.1072414216280855 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 24822 1.1806944892361873 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 23815 1.1327950713544357 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 23465 1.1161468129049688 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 19930 0.9479994025653539 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 16492 0.784465938138877 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 14891 0.7083120473457444 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 11080 0.527036296057407 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10168 0.4836556911833677 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9073 0.4315704254628929 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8731 0.41530269863512814 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8683 0.41301950890491557 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7991 0.38010352362768407 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 7573 0.3602207463937494 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7501 0.35679596179843054 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7060 0.3358191561521023 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5844 0.2779783496533833 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5791 0.27545732765960684 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4813 0.22893733690652526 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4672 0.22223046707402577 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 4031 0.1917403708851451 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 3918 0.18636536172860294 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2807 0.13351903276472396 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2587 0.12305441316791624 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.756645271276236E-5 0.0 0.0 0.0 0.0 8 4.756645271276236E-5 0.0 0.0 0.0 0.0 9 4.756645271276236E-5 0.0 0.0 0.0 0.0 10-11 4.756645271276236E-5 0.0 0.0 3.329651689893366E-4 0.0 12-13 4.756645271276236E-5 0.0 0.0 9.275458278988661E-4 0.0 14-15 7.134967906914354E-5 0.0 0.0 0.002092923919361544 0.0 16-17 9.513290542552473E-5 0.0 0.0 0.0034247845953188903 0.0 18-19 9.513290542552473E-5 0.0 0.0 0.0045425962340688054 0.0 20-21 9.513290542552473E-5 0.0 0.0 0.006207422079015488 0.0 22-23 9.513290542552473E-5 0.0 0.0 0.00984625571154181 0.0 24-25 1.426993581382871E-4 0.0 0.0 0.012771592553376695 0.0 26-27 1.426993581382871E-4 0.0 0.0 0.01610124424327006 0.0 28-29 1.9026581085104945E-4 0.0 0.0 0.021690302437019637 0.0 30-31 2.3783226356381183E-4 0.0 0.0 0.03179817363848164 0.0 32-33 2.3783226356381183E-4 0.0 0.0 0.05153825151427802 0.0 34-35 2.3783226356381183E-4 0.0 0.0 0.08114836832797259 0.0 36-37 2.3783226356381183E-4 0.0 0.0 0.12343494478961833 0.0 38-39 3.3296516898933653E-4 0.0 0.0 0.18959988051307078 0.0 40-41 3.3296516898933653E-4 0.0 0.0 0.28254472911380846 0.0 42-43 3.567483953457177E-4 0.0 0.0 0.35869861990694096 0.0 44-45 3.805316217020989E-4 0.0 0.0 0.4428912412085304 0.0 46-47 3.805316217020989E-4 0.0 0.0 0.5492022630215543 0.0 48-49 3.805316217020989E-4 0.0 0.0 0.6528971299353762 0.0 50-51 3.805316217020989E-4 0.0 0.0 0.7589227530321235 0.0 52-53 3.805316217020989E-4 0.0 0.0 0.8833328101023534 0.0 54-55 3.805316217020989E-4 0.0 0.0 1.02025284423604 0.0 56-57 3.805316217020989E-4 0.0 0.0 1.1751054310424378 0.0 58-59 3.805316217020989E-4 0.0 0.0 1.3530990970935948 0.0 60-61 3.805316217020989E-4 0.0 0.0 1.52426697718047 0.0 62-63 3.805316217020989E-4 0.0 0.0 1.6913917087867607 0.0 64-65 3.805316217020989E-4 0.0 0.0 1.887151444926134 0.0 66-67 4.7566452712762366E-4 0.0 0.0 2.110452157186197 0.0 68-69 4.7566452712762366E-4 0.0 0.0 2.3810814898954584 0.0 70-71 4.994477534840049E-4 0.0 0.0 2.753407898504606 0.0 72-73 5.23230979840386E-4 0.0 0.0 3.057785629413572 0.0 74-75 5.23230979840386E-4 0.0 0.0 3.3474891096606516 0.0 76-77 5.23230979840386E-4 0.0 0.0 3.7004797552420605 0.0 78-79 5.707974325531484E-4 0.0 0.0 4.0536368834079655 0.0 80-81 7.134967906914355E-4 0.0 0.0 4.427461635277565 0.0 82-83 7.134967906914355E-4 0.0 0.0 4.866381077684579 0.0 84-85 7.134967906914355E-4 0.0 0.0 5.297737454110265 0.0 86-87 7.134967906914355E-4 0.0 0.0 5.741437325014912 0.0 88-89 7.134967906914355E-4 0.0 0.0 6.254108552353065 0.0 90-91 7.134967906914355E-4 0.0 0.0 6.747158617947203 0.0 92-93 7.134967906914355E-4 0.0 0.0 7.241825942933575 0.0 94-95 7.134967906914355E-4 0.0 0.0 7.783988370953641 0.0 96-97 7.134967906914355E-4 0.0 0.0 8.331549591356605 0.0 98-99 7.134967906914355E-4 0.0 0.0 8.963065600797595 0.0 100-101 7.134967906914355E-4 0.0 0.0 9.803683736363887 0.0 102-103 7.134967906914355E-4 0.0 0.0 10.528691608611812 0.0 104-105 9.037626015424849E-4 0.0 0.0 11.188723706454102 0.0 106-107 9.037626015424849E-4 0.0 0.0 11.886856532919316 0.0 108-109 9.037626015424849E-4 0.0 0.0 12.550384765035993 0.0 110-111 9.037626015424849E-4 0.0 0.0 13.25729360202671 0.0 112-113 9.037626015424849E-4 0.0 0.0 14.080169450731145 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1670 0.0 34.22694 5 GTGTACG 160 2.591878E-7 29.76675 1 GCGCGAC 85 0.007199466 28.009743 9 AAGTACT 2265 0.0 25.496803 4 CATGGGT 6125 0.0 24.980967 4 GTATAGC 1185 0.0 22.105267 1 CATGGGG 8150 0.0 21.549967 4 AAAGTAC 2565 0.0 21.122057 3 ATGGGTG 2790 0.0 20.913908 5 ATGGGGG 2885 0.0 20.637995 5 TTTACGC 145 0.0038547614 20.529774 3 ATGGGTA 3170 0.0 20.097305 5 GTACAAA 1685 0.0 19.785616 1 ATGGGAG 3145 0.0 19.499788 5 TAGCCCT 1590 0.0 19.096565 4 ATGGGAT 3085 0.0 18.914038 5 ATAGCCC 885 0.0 18.836359 3 GGACCGT 190 8.305365E-4 18.796011 6 GTGTAGC 960 0.0 17.98408 1 ATGGGGC 1560 0.0 17.938519 5 >>END_MODULE