##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364140_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2102322 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08948438916588 33.0 31.0 34.0 30.0 34.0 2 31.912342162618287 33.0 31.0 34.0 30.0 34.0 3 32.14299569713869 34.0 31.0 34.0 30.0 34.0 4 35.610187212044586 37.0 35.0 37.0 33.0 37.0 5 35.744335548978704 37.0 35.0 37.0 33.0 37.0 6 35.88299508828809 37.0 35.0 37.0 35.0 37.0 7 35.86464347516698 37.0 35.0 37.0 35.0 37.0 8 35.9151057735209 37.0 35.0 37.0 35.0 37.0 9 37.57930469262082 39.0 37.0 39.0 35.0 39.0 10-11 37.53750567229949 39.0 37.0 39.0 35.0 39.0 12-13 37.52098298928518 39.0 37.0 39.0 34.5 39.0 14-15 38.808552876295835 40.0 38.0 41.0 34.0 41.0 16-17 38.785578993132354 40.0 38.0 41.0 34.0 41.0 18-19 38.87044563106888 40.0 38.5 41.0 35.0 41.0 20-21 38.936033585720935 40.0 38.5 41.0 35.0 41.0 22-23 38.91183700689048 40.0 38.0 41.0 35.0 41.0 24-25 38.88453790618183 40.0 38.0 41.0 35.0 41.0 26-27 38.71159651090556 40.0 38.0 41.0 34.0 41.0 28-29 38.56194769402594 40.0 38.0 41.0 34.0 41.0 30-31 38.42076855971635 40.0 38.0 41.0 34.0 41.0 32-33 38.13549851069437 40.0 37.0 41.0 34.0 41.0 34-35 37.96107565824836 40.0 37.0 41.0 33.5 41.0 36-37 37.866318289967 40.0 37.0 41.0 33.0 41.0 38-39 37.76680594124021 40.0 37.0 41.0 33.0 41.0 40-41 37.53406138545856 40.0 37.0 41.0 33.0 41.0 42-43 37.349665988369054 40.0 37.0 41.0 32.5 41.0 44-45 37.091608469111776 40.0 36.0 41.0 31.5 41.0 46-47 36.725421224722 40.0 36.0 41.0 31.0 41.0 48-49 36.34468601860229 40.0 36.0 41.0 30.0 41.0 50-51 36.160143165509375 40.0 35.5 41.0 29.5 41.0 52-53 36.04418875890563 39.5 35.0 41.0 29.0 41.0 54-55 35.85986566282425 39.0 35.0 41.0 28.0 41.0 56-57 35.5954639679364 39.0 35.0 41.0 28.0 41.0 58-59 35.44030648016812 39.0 35.0 41.0 27.5 41.0 60-61 35.271071938551756 39.0 35.0 40.5 28.0 41.0 62-63 34.93738732696514 38.0 35.0 40.0 27.5 41.0 64-65 34.65805309557717 38.0 34.5 40.0 26.0 41.0 66-67 34.22618585544936 37.0 34.0 40.0 26.0 41.0 68-69 33.92192466234954 37.0 34.0 39.0 26.0 41.0 70-71 33.48067517725639 36.0 34.0 39.0 25.5 41.0 72-73 33.16609182608563 35.5 34.0 39.0 25.5 40.0 74-75 32.839496994275855 35.0 33.0 37.5 25.0 39.0 76-77 32.354831467301395 35.0 33.0 37.0 24.0 39.0 78-79 31.94012239799612 35.0 33.0 36.0 22.5 39.0 80-81 31.738162850410166 35.0 33.0 36.0 22.5 37.0 82-83 31.526403662236326 35.0 33.0 36.0 21.5 37.0 84-85 31.27919676433962 35.0 33.0 35.0 20.0 36.5 86-87 31.02900649852877 35.0 32.0 35.0 20.0 36.0 88-89 30.826336070307022 35.0 32.0 35.0 18.5 36.0 90-91 30.66054914518328 35.0 32.0 35.0 18.0 36.0 92-93 30.478771805651085 35.0 32.0 35.0 17.5 35.5 94-95 30.401293426982164 35.0 32.0 35.0 14.5 35.0 96-97 30.30967877423154 35.0 32.0 35.0 10.5 35.0 98-99 30.22238101489686 35.0 32.0 35.0 7.0 35.0 100-101 30.003930178155393 35.0 31.0 35.0 6.0 35.0 102-103 28.67453106612593 33.5 28.0 34.5 2.0 35.0 104-105 29.560486452598603 34.0 30.5 35.0 2.0 35.0 106-107 29.597285763075305 34.0 31.0 35.0 2.0 35.0 108-109 29.52879363865288 34.0 31.0 35.0 2.0 35.0 110-111 29.58065248805844 34.5 31.0 35.0 2.0 35.0 112-113 29.51855852719041 35.0 31.0 35.0 2.0 35.0 114-115 29.410381949101993 35.0 31.0 35.0 2.0 35.0 116-117 29.210304843882145 35.0 31.0 35.0 2.0 35.0 118-119 29.022178572074118 34.0 31.0 35.0 2.0 35.0 120-121 28.8354666887375 34.0 31.0 35.0 2.0 35.0 122-123 28.547800955324636 34.0 30.0 35.0 2.0 35.0 124-125 27.526125636320224 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3295489539128411 1101 2 0.6035123396840127 1101 3 0.41845214445285706 1101 4 0.46607192519373797 1101 5 0.2614896290407813 1101 6 0.23617677094210165 1101 7 0.1851950620723315 1101 8 0.16244792998980273 1101 9 0.3306240656316817 1101 10-11 0.17446870322093133 1101 12-13 0.24540389927099682 1101 14-15 0.32064258464325945 1101 16-17 0.38325864639706 1101 18-19 0.2761497555618746 1101 20-21 0.282745593730759 1101 22-23 0.21213196999944017 1101 24-25 0.2748729175537008 1101 26-27 0.33090542692189473 1101 28-29 0.4357632362897945 1101 30-31 0.33009965226919036 1101 32-33 0.34046882240203047 1101 34-35 0.47019252959353963 1101 36-37 0.47322418278702116 1101 38-39 0.3867969911708329 1101 40-41 0.4511974693036507 1101 42-43 0.5125786036528552 1101 44-45 0.5020414562398585 1101 46-47 0.46940303760265323 1101 48-49 0.5373495251767721 1101 50-51 0.5569210421980273 1101 52-53 0.49745472478505093 1101 54-55 0.4938537033542616 1101 56-57 0.5829213211748865 1101 58-59 0.5745444332106828 1101 60-61 0.6372006370267869 1101 62-63 0.6400679094215604 1101 64-65 0.655791408364756 1101 66-67 0.6193197666185313 1101 68-69 0.6351162544788167 1101 70-71 0.5458288367359287 1101 72-73 0.49229498846610653 1101 74-75 0.4730850609494155 1101 76-77 0.5451903418465562 1101 78-79 0.54661817995067 1101 80-81 0.5270059242779155 1101 82-83 0.5263465245070904 1101 84-85 0.4966850945629844 1101 86-87 0.5550608692520385 1101 88-89 0.4931949457201448 1101 90-91 0.4712948600730904 1101 92-93 0.44360734941897206 1101 94-95 0.422480108624228 1101 96-97 0.45220264607909044 1101 98-99 0.4546760273575039 1101 100-101 0.39779745797808985 1101 102-103 0.32696688493284043 1101 104-105 0.3727740902291323 1101 106-107 0.30727714947187224 1101 108-109 0.3869764008679546 1101 110-111 0.3199547441020485 1101 112-113 0.25473613226375846 1101 114-115 0.29818595368760725 1101 116-117 0.30442210543915493 1101 118-119 0.35201017628967435 1101 120-121 0.32592828330741597 1101 122-123 0.25795833384375655 1101 124-125 0.34219065246459124 1102 1 0.28037083734341905 1102 2 0.6199703985333791 1102 3 0.38814650001677364 1102 4 0.4592652455609709 1102 5 0.26458214843764694 1102 6 0.23706348487229434 1102 7 0.16445819024850294 1102 8 0.158563328279115 1102 9 0.2235967531695806 1102 10-11 0.19922585026068163 1102 12-13 0.19306085181925425 1102 14-15 0.3447362647152019 1102 16-17 0.34649635474345786 1102 18-19 0.23717852148195107 1102 20-21 0.20118333553806877 1102 22-23 0.19215554947953706 1102 24-25 0.2286621034081051 1102 26-27 0.294993774162414 1102 28-29 0.34434386660955596 1102 30-31 0.30874375373937823 1102 32-33 0.2778070075663024 1102 34-35 0.30845716351034724 1102 36-37 0.35272441283072453 1102 38-39 0.4187199997242743 1102 40-41 0.3857301668798243 1102 42-43 0.4378747849272955 1102 44-45 0.5031587367049681 1102 46-47 0.5452411286581409 1102 48-49 0.5414842994202402 1102 50-51 0.5688328622620205 1102 52-53 0.4431113427242721 1102 54-55 0.42920723870779653 1102 56-57 0.45994413732813655 1102 58-59 0.4625256951049863 1102 60-61 0.382961558874662 1102 62-63 0.3572683820568159 1102 64-65 0.42816159051754 1102 66-67 0.4247396486688473 1102 68-69 0.44189261016821035 1102 70-71 0.40639437827973524 1102 72-73 0.3415193751904937 1102 74-75 0.2703522462600745 1102 76-77 0.29464686358569026 1102 78-79 0.28603695360857273 1102 80-81 0.18426223479509574 1102 82-83 0.28011636577693366 1102 84-85 0.3074286585674173 1102 86-87 0.32617783602118067 1102 88-89 0.4194149616140699 1102 90-91 0.3692338775773294 1102 92-93 0.3583406032826595 1102 94-95 0.3646486888362901 1102 96-97 0.31374508914327137 1102 98-99 0.3667823389203413 1102 100-101 0.38587518138189836 1102 102-103 0.2014171327526526 1102 104-105 0.1941043702550651 1102 106-107 0.274407824754725 1102 108-109 0.4338096400924947 1102 110-111 0.40155856701021975 1102 112-113 0.36532520100499966 1102 114-115 0.2825340934705274 1102 116-117 0.31214190174590684 1102 118-119 0.32034910386773063 1102 120-121 0.3184150035115252 1102 122-123 0.3498356996341663 1102 124-125 0.5168884586841394 1103 1 0.24139888359051298 1103 2 0.6056526471283128 1103 3 0.42288882556781715 1103 4 0.506394791054916 1103 5 0.2034652753071171 1103 6 0.19370372783574652 1103 7 0.22087254165418102 1103 8 0.21994239878889488 1103 9 0.27053610103136094 1103 10-11 0.2466464542223008 1103 12-13 0.2082226115909691 1103 14-15 0.4305190681977038 1103 16-17 0.3907005790685645 1103 18-19 0.3092410388827318 1103 20-21 0.24191515279741083 1103 22-23 0.18790664655783473 1103 24-25 0.18573679243753105 1103 26-27 0.12248309402995972 1103 28-29 0.25254148653859687 1103 30-31 0.2791980048349245 1103 32-33 0.2837232247444632 1103 34-35 0.3669729182991972 1103 36-37 0.3077544008273705 1103 38-39 0.38152065730837137 1103 40-41 0.32172912257754405 1103 42-43 0.3114827473242485 1103 44-45 0.25551953294466045 1103 46-47 0.42767064080376116 1103 48-49 0.6368542873556962 1103 50-51 0.7523538946527282 1103 52-53 0.713579820398742 1103 54-55 0.7199749538580349 1103 56-57 0.7308840297772434 1103 58-59 0.6010888514270505 1103 60-61 0.6477748949411364 1103 62-63 0.6891025195232956 1103 64-65 0.701254795515915 1103 66-67 0.61451471574793 1103 68-69 0.7286332346487399 1103 70-71 0.7492597234552889 1103 72-73 0.686375472579833 1103 74-75 0.6896669432849336 1103 76-77 0.7387859719922218 1103 78-79 0.7586553274137202 1103 80-81 0.6987653396567097 1103 82-83 0.567372716491171 1103 84-85 0.5605227610130648 1103 86-87 0.5886494466024921 1103 88-89 0.691627189537126 1103 90-91 0.6971574395455491 1103 92-93 0.60536474971342 1103 94-95 0.6636809755217392 1103 96-97 0.6969393691407042 1103 98-99 0.7503128556969934 1103 100-101 0.7478993431100562 1103 102-103 0.5465375691567864 1103 104-105 0.6806522475373065 1103 106-107 0.7841597111786918 1103 108-109 0.7615367834872337 1103 110-111 0.7171381496768419 1103 112-113 0.7302467916487032 1103 114-115 0.7348265613383482 1103 116-117 0.7550954109177148 1103 118-119 0.7897176477289349 1103 120-121 0.7813983120120831 1103 122-123 0.7803557907250678 1103 124-125 0.7361053033615086 1104 1 0.20604278579345703 1104 2 0.5592599979136601 1104 3 0.04709993731142248 1104 4 0.38193505517368465 1104 5 0.1710424861429658 1104 6 0.19243254147911415 1104 7 0.1406370597864921 1104 8 0.12257357008725478 1104 9 0.230591018503425 1104 10-11 0.1928077748915058 1104 12-13 0.17235230711119698 1104 14-15 0.22992074384703898 1104 16-17 0.2609585967755166 1104 18-19 0.18623253607607637 1104 20-21 0.1626410936270375 1104 22-23 0.0835879451235968 1104 24-25 0.038176928763284934 1104 26-27 0.13976678557576605 1104 28-29 0.25204438702130716 1104 30-31 0.2725420282458657 1104 32-33 0.2802453628049406 1104 34-35 0.28311731229596404 1104 36-37 0.24354486195126412 1104 38-39 0.2871122607743928 1104 40-41 0.42308992072945273 1104 42-43 0.388088088687077 1104 44-45 0.3705164278701645 1104 46-47 0.4386411436869935 1104 48-49 0.4478756456269437 1104 50-51 0.4451320130311416 1104 52-53 0.46719865529863114 1104 54-55 0.3191818359169716 1104 56-57 0.4587698697450193 1104 58-59 0.39220237814704717 1104 60-61 0.3581264853719901 1104 62-63 0.45372959224234677 1104 64-65 0.41550533208587836 1104 66-67 0.36729529954160967 1104 68-69 0.4391824748228288 1104 70-71 0.3888332681016209 1104 72-73 0.38524349392243806 1104 74-75 0.40915961120342104 1104 76-77 0.2998293960275831 1104 78-79 0.37069169099959964 1104 80-81 0.41476333598615156 1104 82-83 0.3497095731402666 1104 84-85 0.31525747895904743 1104 86-87 0.39819215074849623 1104 88-89 0.3855931402284192 1104 90-91 0.35028481781343856 1104 92-93 0.32377500512687263 1104 94-95 0.41309055517736226 1104 96-97 0.3900365237579422 1104 98-99 0.3496172238059039 1104 100-101 0.41962508619078065 1104 102-103 0.3511571286071131 1104 104-105 0.3838301340913155 1104 106-107 0.46543943425880485 1104 108-109 0.5259196452214674 1104 110-111 0.4900884782038375 1104 112-113 0.3401709409564724 1104 114-115 0.29855313270339323 1104 116-117 0.33435766862323035 1104 118-119 0.3912051380770265 1104 120-121 0.39542847853968155 1104 122-123 0.2655756460648888 1104 124-125 0.3019444361065702 1105 1 0.2164416908055813 1105 2 0.5518433509460614 1105 3 0.14120885772680936 1105 4 0.39952011812469124 1105 5 0.12074018053271374 1105 6 0.07469744566967762 1105 7 0.09742781654646393 1105 8 0.01324987389519805 1105 9 0.1283554849148203 1105 10-11 0.19675208714224368 1105 12-13 0.19348425123065738 1105 14-15 0.22049144268176946 1105 16-17 0.3030652924968038 1105 18-19 0.1821740792586155 1105 20-21 0.16973100614720948 1105 22-23 0.15854457718791082 1105 24-25 0.13204791050456066 1105 26-27 0.12428607245771417 1105 28-29 0.14210332021608707 1105 30-31 0.14981072025612008 1105 32-33 0.123631317633496 1105 34-35 0.2410061667864909 1105 36-37 0.2613673136025625 1105 38-39 0.2426052793067086 1105 40-41 0.18345352742177568 1105 42-43 0.1057436282833919 1105 44-45 0.3374998994569225 1105 46-47 0.29187197989919156 1105 48-49 0.462580695666567 1105 50-51 0.4414840366748152 1105 52-53 0.32563119390979267 1105 54-55 0.5461293332161006 1105 56-57 0.4248255854929468 1105 58-59 0.42087846316540833 1105 60-61 0.34992807561629036 1105 62-63 0.3334164191493798 1105 64-65 0.34695219521519505 1105 66-67 0.4393656689920249 1105 68-69 0.48246121475480663 1105 70-71 0.3350869764251101 1105 72-73 0.31613623342803976 1105 74-75 0.27794150718516875 1105 76-77 0.26797131247235484 1105 78-79 0.3040390636646535 1105 80-81 0.2779459484938016 1105 82-83 0.26012913716901664 1105 84-85 0.3429706098140173 1105 86-87 0.3265748597315472 1105 88-89 0.35369920837957736 1105 90-91 0.3047397357593482 1105 92-93 0.29611477175398093 1105 94-95 0.34589695359279915 1105 96-97 0.42988007720479615 1105 98-99 0.4764663857857947 1105 100-101 0.361424906519332 1105 102-103 0.09381537807443507 1105 104-105 0.33239078260444543 1105 106-107 0.3024868408022563 1105 108-109 0.32883712634936657 1105 110-111 0.31336794010145397 1105 112-113 0.4725229909988897 1105 114-115 0.42774343324508735 1105 116-117 0.3498619306203601 1105 118-119 0.26996040932611365 1105 120-121 0.33021188284963543 1105 122-123 0.33452946386316285 1105 124-125 0.3413887085592009 1106 1 0.231481398444906 1106 2 0.519292053329 1106 3 0.4259648562824552 1106 4 0.3977601136656048 1106 5 0.19750969689197007 1106 6 0.10029778591700733 1106 7 0.07243332981344963 1106 8 0.021156868110502103 1106 9 0.20396721520822325 1106 10-11 0.173057156197423 1106 12-13 0.16790784219713828 1106 14-15 0.24581642250685576 1106 16-17 0.2377046036655912 1106 18-19 0.18470806158223496 1106 20-21 0.16956954618564168 1106 22-23 0.14111114231352673 1106 24-25 0.10845627758393306 1106 26-27 0.19382805931795843 1106 28-29 0.21290699701989269 1106 30-31 0.15178307637197008 1106 32-33 0.19318451278375903 1106 34-35 0.10481059969548312 1106 36-37 0.1077852652845337 1106 38-39 0.14743478001071253 1106 40-41 0.14698962780927616 1106 42-43 0.1519987265407181 1106 44-45 0.12266727254878873 1106 46-47 0.18311127749685596 1106 48-49 0.21715034579828085 1106 50-51 0.23090419851040878 1106 52-53 0.14266827941511195 1106 54-55 0.0809677278496963 1106 56-57 0.17806084325938087 1106 58-59 0.11329723638473865 1106 60-61 0.047247294986156874 1106 62-63 0.05068033621045487 1106 64-65 0.06650199758578168 1106 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112-113 -1.2014187343749825 2216 114-115 -1.2418503540906336 2216 116-117 -1.2133652147675171 2216 118-119 -1.4227681367950922 2216 120-121 -1.4345328952989256 2216 122-123 -1.506571759718593 2216 124-125 -1.7928149333474437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43.0 3 9.0 4 45.0 5 211.0 6 619.0 7 1201.0 8 2129.0 9 4266.0 10 8302.0 11 11499.0 12 11801.0 13 15847.0 14 23170.0 15 23546.0 16 12957.0 17 10694.0 18 10507.0 19 10158.0 20 10073.0 21 9879.0 22 10284.0 23 11119.0 24 12614.0 25 14641.0 26 17583.0 27 21449.0 28 26902.0 29 33620.0 30 42626.0 31 53861.0 32 70232.0 33 93541.0 34 132944.0 35 207992.0 36 346763.0 37 569627.0 38 267660.0 39 1908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.42240241768062 18.340874294136533 12.156667152565594 23.080056135617248 2 13.200832222656663 22.85192277871801 37.639524297419705 26.307720701205618 3 22.336086335466064 28.397844086816843 28.516713274106447 20.749356303610643 4 13.311519358119261 18.962318807490004 34.35696339571198 33.36919843867875 5 11.655255474660875 39.21915862555784 34.43073896386947 14.694846935911816 6 31.379406301358788 31.37541057298269 19.146150900584853 18.09903222507367 7 23.825179967673837 33.85171253499702 22.971552407290606 19.351555090038538 8 27.75297980043019 32.31403181815155 22.318797976713366 17.614190404704893 9 29.25798236426199 15.01915501050743 20.80666044497465 34.91620218025593 10-11 23.921067220721103 27.617696730826722 25.82356020406974 22.637675844382436 12-13 27.40110064964873 25.518037420565854 26.24091849874394 20.83994343104148 14-15 27.316350843345067 23.633806675885218 26.0123637017471 23.037478779022617 16-17 21.980408679316092 27.63017416330667 30.86587910385179 19.523538053525446 18-19 23.450332537070913 25.243159706267644 32.842495107790334 18.464012648871105 20-21 21.389060656798204 25.368616575973213 32.81132272917539 20.4310000380532 22-23 19.958098711805327 26.74088460283439 29.16218352849849 24.138833156861793 24-25 21.177107978701645 27.68988290090671 27.98700674777698 23.14600237261466 26-27 22.682681339966 24.45341389187765 29.14577310231258 23.718131665843767 28-29 22.113427071045322 23.59039061608128 32.036868792572015 22.259313520301383 30-31 23.98664429140731 25.448813264571267 30.547960778605756 20.01658166541567 32-33 19.450017647153956 25.744391201728373 32.27524137596429 22.530349775153375 34-35 18.069567839750523 26.90636829182209 33.20361961678563 21.820444251641756 36-37 23.084522732483414 25.47478454775244 30.15489539661384 21.28579732315031 38-39 24.419522794319803 23.968545256150104 30.01830832764914 21.593623621880948 40-41 21.444774608634933 26.210221940417284 29.91743411210752 22.42756933884026 42-43 23.910419050935108 25.405908324224356 29.426700572034157 21.256972052806372 44-45 25.63368028303942 23.431591354702086 29.110455011173357 21.824273351085132 46-47 22.108792087986522 25.553578376671126 32.807676464404594 19.529953070937754 48-49 23.957367139762606 25.26696671585038 31.40660659975018 19.369059544636833 50-51 21.015405822704608 26.274376617853974 30.96083758815253 21.74937997128889 52-53 20.059938487063352 27.555864420388502 27.329257839664905 25.05493925288324 54-55 21.424667581845217 28.11327189650301 26.07554884551463 24.38651167613715 56-57 23.01483787925922 25.203370368573413 26.96734848419985 24.814443267967516 58-59 22.670147579676186 24.164828223269318 29.860768236264473 23.304255960790023 60-61 24.247118186462398 26.07443103387588 28.513400896722768 21.16504988293896 62-63 19.956077137565035 26.343704722682826 30.181342344322136 23.518875795430006 64-65 18.411094970228156 27.15932668734856 31.351619780414225 23.077958562009055 66-67 23.12122025075131 26.178221033695127 28.266007776163686 22.434550939389876 68-69 24.47705441887589 24.547714384380697 28.252403770687838 22.72282742605557 70-71 21.528766763607095 26.544340020225256 28.24591094989255 23.680982266275098 72-73 23.93755571220774 25.801280679172837 27.896606704396376 22.364556904223047 74-75 25.360839110279016 23.83395597819934 27.7884168077012 23.016788103820442 76-77 21.884540046672203 25.77045761781497 31.734791340241884 20.610210995270943 78-79 23.48565062821014 25.394088060725235 30.66055057217686 20.45971073888776 80-81 20.576866911919296 26.347129507278144 30.357029037416723 22.718974543385837 82-83 19.750304663129626 27.32388283050836 27.023619597759048 25.902192908602963 84-85 21.024776413889022 27.664078100310036 26.003509452881147 25.30763603291979 86-87 22.43621576523482 24.76742382946095 27.0221212544986 25.774239150805634 88-89 22.229087646897096 23.90352191529176 29.45084054678589 24.41654989102526 90-91 23.53640403325466 25.81086531939446 28.061852561120514 22.59087808623037 92-93 19.670916253552026 26.06922250730383 29.4713892543578 24.788471984786344 94-95 18.187524051027896 26.84960431451062 30.613646666200513 24.34922496826097 96-97 22.6332398150183 25.698152751655122 27.914765143450243 23.753842289876335 98-99 23.668010391770114 24.35304143365138 27.754697904450037 24.224250270128465 100-101 21.01866912363309 26.01733369515557 27.889835117987428 25.074162063223916 102-103 23.178947974291226 25.190683554371425 27.719629471332834 23.91073900000452 104-105 24.481795182571524 23.287679053784824 27.754130592252768 24.476395171390884 106-107 21.224330662716046 25.168004829804346 31.250551571553725 22.35711293592588 108-109 22.739179686217682 25.06152757762846 29.887392520016387 22.311900216137474 110-111 19.96400058326001 25.679425360440177 30.04300472150405 24.313569334795766 112-113 19.16168391096192 26.575149042613948 26.9032889874617 27.359878058962433 114-115 20.12992853074263 27.1733311507269 25.932481323725426 26.764258994805047 116-117 21.53394674579865 24.49934997820571 26.75113301749063 27.215570258505007 118-119 21.09755842267577 23.776190172601314 28.987057994003347 26.139193410719567 120-121 22.35114822666154 25.36640875651277 27.781462057521704 24.500980959303988 122-123 19.032858264441092 25.69500914850305 28.78447515800284 26.48765742905302 124-125 17.769596213648228 26.574410017659332 29.75730008383725 25.89869368485519 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1866.0 1 1340.5 2 1854.5 3 2474.0 4 1376.5 5 872.0 6 1680.5 7 3624.0 8 5507.0 9 5873.5 10 4613.0 11 3105.0 12 2608.5 13 2639.5 14 2961.0 15 3442.5 16 3745.5 17 4073.5 18 4490.0 19 4728.5 20 5009.0 21 5592.5 22 6266.0 23 6999.5 24 8094.0 25 9442.5 26 11319.5 27 13382.0 28 15047.0 29 16945.5 30 19899.5 31 23015.0 32 26068.5 33 29393.5 34 33115.0 35 35950.0 36 38786.0 37 41802.5 38 42297.5 39 41929.0 40 41686.0 41 42706.5 42 44163.0 43 45929.5 44 47566.5 45 49150.0 46 52641.0 47 60938.5 48 74291.5 49 120707.5 50 285296.0 51 282798.0 52 108731.0 53 55709.0 54 44985.5 55 38612.0 56 33523.0 57 29123.0 58 26514.0 59 23734.0 60 20157.5 61 16804.5 62 14390.5 63 11732.0 64 9168.0 65 7131.5 66 5094.0 67 3897.5 68 2902.5 69 1955.0 70 1536.5 71 1361.5 72 1272.0 73 1204.5 74 907.0 75 711.0 76 468.0 77 217.5 78 135.5 79 89.5 80 44.5 81 16.0 82 10.5 83 4.0 84 3.0 85 1.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14840733246381857 2 0.0 3 5.23230979840386E-4 4 0.0 5 0.0 6 0.003662616858882702 7 0.0 8 0.0 9 0.0 10-11 7.134967906914355E-5 12-13 0.005184743345691098 14-15 0.0018550916557977322 16-17 6.421471116222918E-4 18-19 0.0 20-21 9.513290542552473E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 9.513290542552473E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.756645271276236E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.988955069680098E-4 96-97 0.002853987162765742 98-99 0.0038766658960901327 100-101 2.378322635638118E-5 102-103 0.003924232348802895 104-105 0.02257028181220574 106-107 0.017766070088216745 108-109 0.01993034368664743 110-111 0.01633907650683387 112-113 0.015340180999865862 114-115 0.040859582880262875 116-117 0.04062175061669906 118-119 0.023022163112976987 120-121 0.014174802908403185 122-123 0.0031156026526859347 124-125 0.0016410426185903015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2102322.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.01526644608221 #Duplication Level Percentage of deduplicated Percentage of total 1 85.07310057666582 41.69880692159625 2 9.827276159440684 9.633731187884331 3 2.4404572254319796 3.5885898346444502 4 0.9615621305337801 1.8852489613030285 5 0.45122879624841195 1.1058549838130425 6 0.24523560991759752 0.7212173257307114 7 0.17310732592992148 0.5939431192956727 8 0.12797109810780213 0.5018029976921318 9 0.0870467753881492 0.3839958800030164 >10 0.5283203034054103 4.922318660041643 >50 0.04665993754322334 1.5971119873771982 >100 0.029582057628250086 2.8463457336266953 >500 0.003565689085865394 1.2318921410232768 >1k 0.0022450634985078407 2.5747328990248466 >5k 0.0010565004698860428 4.235531420502132 >10k+ 0.0015847507048290643 22.478875946441608 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 81514 3.877331826428111 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 70861 3.370606405679054 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 44877 2.134639698390637 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 24967 1.187591624879538 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 23128 1.100116918340768 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 22300 1.0607318954946008 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 19090 0.9080435822866335 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 14518 0.690569760483884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12026 0.5720341603236802 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 11903 0.5661834866400105 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 11307 0.537833880823204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10776 0.5125760944327272 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9676 0.4602529964486886 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9672 0.4600627306378376 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9168 0.4360892384706053 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 8928 0.4246732898195424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7248 0.3447616492621016 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7222 0.3435249214915698 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6833 0.3250215713863052 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6686 0.31802930283752917 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4923 0.2341696467049291 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4677 0.22246829933758958 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 4628 0.22013754315466424 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4025 0.1914549721688685 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2846 0.13537412442052169 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2590 0.12319711252605453 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2329 0.11078226836802356 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2233 0.10621588890759835 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.513290542552473E-5 0.0 0.0 0.0 0.0 6 9.513290542552473E-5 0.0 0.0 0.0 0.0 7 9.513290542552473E-5 0.0 0.0 0.0 0.0 8 9.513290542552473E-5 0.0 0.0 0.0 0.0 9 9.513290542552473E-5 0.0 0.0 0.0 0.0 10-11 9.513290542552473E-5 0.0 0.0 4.043148480584801E-4 0.0 12-13 9.513290542552473E-5 0.0 0.0 9.988955069680098E-4 0.0 14-15 9.513290542552473E-5 0.0 0.0 0.00221184005114345 0.0 16-17 9.513290542552473E-5 0.0 0.0 0.0034723510480316527 0.0 18-19 9.513290542552473E-5 0.0 0.0 0.004709078818563474 0.0 20-21 9.513290542552473E-5 0.0 0.0 0.006373904663510157 0.0 22-23 9.513290542552473E-5 0.0 0.0 0.010012738296036477 0.0 24-25 9.513290542552473E-5 0.0 0.0 0.013223473854147938 0.0 26-27 9.513290542552473E-5 0.0 0.0 0.016529342317684922 0.0 28-29 9.513290542552473E-5 0.0 0.0 0.022189750190503642 0.0 30-31 9.513290542552473E-5 0.0 0.0 0.03227383816560926 0.0 32-33 1.1891613178190592E-4 0.0 0.0 0.052227965078613076 0.0 34-35 1.426993581382871E-4 0.0 0.0 0.08221861351400975 0.0 36-37 1.426993581382871E-4 0.0 0.0 0.12467167256015016 0.0 38-39 2.3783226356381183E-4 0.0 0.0 0.19093174118902811 0.0 40-41 2.3783226356381183E-4 0.0 0.0 0.2842809046378243 0.0 42-43 2.3783226356381183E-4 0.0 0.0 0.361386124485212 0.0 44-45 2.3783226356381183E-4 0.0 0.0 0.4461257599929982 0.0 46-47 2.3783226356381183E-4 0.0 0.0 0.5526508308432296 0.0 48-49 3.3296516898933653E-4 0.0 0.0 0.6562029983989133 0.0 50-51 3.3296516898933653E-4 0.0 0.0 0.7623475376274425 0.0 52-53 3.3296516898933653E-4 0.0 0.0 0.8877089237519276 0.0 54-55 3.567483953457177E-4 0.0 0.0 1.0255089372608002 0.0 56-57 3.805316217020989E-4 0.0 0.0 1.1816220350640863 0.0 58-59 3.805316217020989E-4 0.0 0.0 1.3608286456594185 0.0 60-61 3.805316217020989E-4 0.0 0.0 1.5334235193276768 0.0 62-63 3.805316217020989E-4 0.0 0.0 1.7019276780626376 0.0 64-65 3.805316217020989E-4 0.0 0.0 1.8982819948609206 0.0 66-67 4.280980744148613E-4 0.0 0.0 2.1226053858543077 0.0 68-69 4.280980744148613E-4 0.0 0.0 2.3949233276348725 0.0 70-71 4.280980744148613E-4 0.0 0.0 2.767987016261068 0.0 72-73 4.280980744148613E-4 0.0 0.0 3.0730306775080125 0.0 74-75 4.280980744148613E-4 0.0 0.0 3.3647795152217403 0.0 76-77 4.280980744148613E-4 0.0 0.0 3.720314966023283 0.0 78-79 4.5188130077124244E-4 0.0 0.0 4.076230948446527 0.0 80-81 4.7566452712762366E-4 0.0 0.0 4.451863225519212 0.0 82-83 4.7566452712762366E-4 0.0 0.0 4.893446389278141 0.0 84-85 4.994477534840049E-4 0.0 0.0 5.326015710248003 0.0 86-87 5.23230979840386E-4 0.0 0.0 5.772569568315415 0.0 88-89 5.23230979840386E-4 0.0 0.0 6.288427747985323 0.0 90-91 5.23230979840386E-4 0.0 0.0 6.785378262701908 0.0 92-93 5.23230979840386E-4 0.0 0.0 7.283708204547162 0.0 94-95 5.23230979840386E-4 0.0 0.0 7.830651061064861 0.0 96-97 5.23230979840386E-4 0.0 0.0 8.383159192549952 0.0 98-99 5.23230979840386E-4 0.0 0.0 9.020430742769186 0.0 100-101 5.23230979840386E-4 0.0 0.0 9.86611470554939 0.0 102-103 5.470142061967672E-4 0.0 0.0 10.599327790890264 0.0 104-105 6.659303379786731E-4 0.0 0.0 11.268611563785186 0.0 106-107 6.659303379786731E-4 0.0 0.0 11.976162547887526 0.0 108-109 6.659303379786731E-4 0.0 0.0 12.649037587962262 0.0 110-111 6.659303379786731E-4 0.0 0.0 13.365507281948247 0.0 112-113 6.659303379786731E-4 0.0 0.0 14.202795765824646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCA 1175 0.0 40.565018 1 AGTACTC 1755 0.0 36.613956 5 AAAGTAC 2045 0.0 31.7127 3 TTATCAA 1600 0.0 30.864422 2 AAGTACT 2305 0.0 28.651814 4 TATAACG 120 0.0012885104 24.790699 2 AAAAGTA 2955 0.0 21.745346 2 GTATAGG 550 0.0 21.66541 1 ACCGTCC 170 3.933635E-4 21.00118 8 TAGCCCT 1310 0.0 20.892315 4 ATAGCCC 570 0.0 20.876379 3 CATGGGG 5700 0.0 20.771997 4 GTGTAGC 870 0.0 20.544783 1 AAAAAGT 3130 0.0 20.177208 1 GAGTACT 16040 0.0 19.938608 12-13 ACACCGA 180 5.7817774E-4 19.83539 6 ATAACGC 150 0.004708236 19.832561 3 CATGGGT 4955 0.0 18.851938 4 AGTACTT 16240 0.0 18.667185 12-13 GTATAGC 900 0.0 18.53596 1 >>END_MODULE