##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364139_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1930041 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.321192140477844 31.0 31.0 34.0 26.0 34.0 2 30.32947952919135 31.0 31.0 34.0 26.0 34.0 3 30.525274851674137 33.0 31.0 34.0 26.0 34.0 4 33.728849801636336 37.0 35.0 37.0 28.0 37.0 5 33.90427923551883 37.0 35.0 37.0 30.0 37.0 6 33.68292487050793 37.0 35.0 37.0 28.0 37.0 7 33.676748835905556 37.0 35.0 37.0 28.0 37.0 8 33.476041182544826 37.0 35.0 37.0 27.0 37.0 9 34.8358584092255 39.0 35.0 39.0 25.0 39.0 10-11 34.92577877879278 39.0 35.0 39.0 27.0 39.0 12-13 34.856521700834335 39.0 35.0 39.0 26.5 39.0 14-15 36.03149544491542 40.0 37.0 41.0 26.5 41.0 16-17 36.07577896013608 40.0 37.0 41.0 27.0 41.0 18-19 35.97295031556324 40.0 36.0 41.0 27.0 41.0 20-21 35.98110454648373 40.0 36.0 41.0 27.0 41.0 22-23 35.98666686355368 40.0 36.0 41.0 27.0 41.0 24-25 35.938271259522466 40.0 36.0 41.0 27.0 41.0 26-27 35.67993244703092 40.0 36.0 41.0 25.0 41.0 28-29 35.501441161094505 40.0 35.5 41.0 25.0 41.0 30-31 35.494367477167586 40.0 35.0 41.0 25.0 41.0 32-33 35.10127738219033 39.0 35.0 41.0 24.5 41.0 34-35 35.11666436101616 39.0 35.0 41.0 25.0 41.0 36-37 35.09225684842964 39.5 35.0 41.0 25.0 41.0 38-39 34.89865966577912 39.0 35.0 41.0 24.5 41.0 40-41 34.459273663098344 39.0 33.5 41.0 23.5 41.0 42-43 34.14177988965001 38.0 33.0 41.0 21.5 41.0 44-45 33.85382072194321 38.0 33.0 40.0 18.0 41.0 46-47 33.525083664025786 38.0 33.0 40.0 15.0 41.0 48-49 33.183289111474835 38.0 32.5 40.0 13.0 41.0 50-51 33.06194583431129 38.0 32.0 40.0 11.0 41.0 52-53 32.891128478617816 38.0 32.0 40.0 8.5 41.0 54-55 32.5251909156334 37.0 31.0 40.0 6.5 41.0 56-57 32.07318756440925 37.0 31.0 40.0 2.0 41.0 58-59 31.62797396531991 36.0 30.0 40.0 2.0 41.0 60-61 31.32134213729138 36.0 29.5 39.0 2.0 41.0 62-63 30.64666916402294 35.0 28.5 39.0 2.0 41.0 64-65 30.37889557786596 35.0 28.0 39.0 2.0 40.0 66-67 29.91683725889761 35.0 28.5 37.5 2.0 40.0 68-69 29.488127713349094 35.0 28.0 37.0 2.0 40.0 70-71 28.802490724290315 34.0 26.0 36.5 2.0 39.0 72-73 28.560567366185484 34.0 26.0 36.0 2.0 39.0 74-75 28.2396617481183 34.0 26.0 36.0 2.0 39.0 76-77 27.646088347345987 33.0 26.0 35.0 2.0 37.0 78-79 27.320858986933438 33.0 26.0 35.0 2.0 37.0 80-81 27.133793530811005 33.0 25.5 35.0 2.0 36.0 82-83 26.95615585368394 33.0 25.5 35.0 2.0 36.0 84-85 26.681842510081392 33.0 25.0 35.0 2.0 36.0 86-87 26.300302169746654 33.0 24.0 35.0 2.0 35.0 88-89 25.945430951984957 32.5 23.0 35.0 2.0 35.0 90-91 25.826106802912477 33.0 21.5 35.0 2.0 35.0 92-93 25.40814832431021 32.0 19.5 35.0 2.0 35.0 94-95 25.35616108673339 32.0 19.5 35.0 2.0 35.0 96-97 25.30127753762744 32.0 19.5 35.0 2.0 35.0 98-99 25.050334422947493 32.0 18.0 35.0 2.0 35.0 100-101 24.4461806769908 32.0 14.0 35.0 2.0 35.0 102-103 23.145633175668287 29.5 10.0 34.0 2.0 35.0 104-105 23.942519614868285 31.0 10.0 35.0 2.0 35.0 106-107 23.942747071176207 31.0 7.0 35.0 2.0 35.0 108-109 23.937008851107308 31.0 7.0 35.0 2.0 35.0 110-111 23.971094396440282 32.0 4.5 35.0 2.0 35.0 112-113 23.88856065751971 32.0 2.0 35.0 2.0 35.0 114-115 23.66963577457681 31.5 2.0 35.0 2.0 35.0 116-117 23.22326805492733 31.0 2.0 35.0 2.0 35.0 118-119 22.910038439597916 31.0 2.0 35.0 2.0 35.0 120-121 22.70335992862328 31.0 2.0 35.0 2.0 35.0 122-123 22.07110677959691 31.0 2.0 35.0 2.0 35.0 124-125 20.998517389008832 29.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6132496143403614 1101 2 0.6209550809333244 1101 3 0.5696155340399756 1101 4 0.7110738255499882 1101 5 0.615538757795072 1101 6 0.6408694932550745 1101 7 0.7270938428951013 1101 8 0.6273594738993893 1101 9 0.7981492668993866 1101 10-11 0.668351063195999 1101 12-13 0.7330186244085084 1101 14-15 0.7414587300247248 1101 16-17 0.7161190739232524 1101 18-19 0.7694733640212377 1101 20-21 0.7401867748167064 1101 22-23 0.766886473285723 1101 24-25 0.8364272557245371 1101 26-27 0.985590418884911 1101 28-29 1.0939094976270596 1101 30-31 1.0035423198482079 1101 32-33 1.1181961151551008 1101 34-35 1.0693420968666416 1101 36-37 1.0680677545889523 1101 38-39 0.9695041125735244 1101 40-41 1.1301571210977457 1101 42-43 1.0355746601628795 1101 44-45 1.0410374960092028 1101 46-47 1.09581589474287 1101 48-49 1.1605277927274003 1101 50-51 1.1365308571115307 1101 52-53 1.0841051975178857 1101 54-55 1.20684323556047 1101 56-57 1.043898994695823 1101 58-59 1.2151851961130014 1101 60-61 1.2287495449364627 1101 62-63 1.2427272444931532 1101 64-65 1.2190012886887303 1101 66-67 1.1994805496807004 1101 68-69 1.0823589663510127 1101 70-71 1.2572589747547696 1101 72-73 1.1730102977642929 1101 74-75 1.2815509806057719 1101 76-77 1.1962329186047143 1101 78-79 1.1263044132038758 1101 80-81 1.1527797624483256 1101 82-83 1.1312214263078246 1101 84-85 1.1794225856680178 1101 86-87 1.0995758278300762 1101 88-89 1.210090136892216 1101 90-91 1.0416768939040608 1101 92-93 1.1069462181745742 1101 94-95 1.1353852889412757 1101 96-97 1.0238293740610125 1101 98-99 0.97107384747137 1101 100-101 1.1123177195834515 1101 102-103 1.0629729976339597 1101 104-105 1.0462102991330369 1101 106-107 1.0070601325953206 1101 108-109 1.0641802983057111 1101 110-111 1.123663790158055 1101 112-113 1.179176406497966 1101 114-115 1.2542695800268469 1101 116-117 0.9938857397541092 1101 118-119 1.1013549609143034 1101 120-121 1.2606243519072677 1101 122-123 1.3294901927601401 1101 124-125 1.252314271182147 1102 1 0.38574550165673216 1102 2 0.4282774468626869 1102 3 0.46912589966179397 1102 4 0.4853590258442111 1102 5 0.3191139046686686 1102 6 0.4556411806581906 1102 7 0.41040500791341117 1102 8 0.4715213574472017 1102 9 0.5884668451908013 1102 10-11 0.4197718225199054 1102 12-13 0.44214098818356007 1102 14-15 0.5480738889426391 1102 16-17 0.547312895568659 1102 18-19 0.6302669934133149 1102 20-21 0.637293849944939 1102 22-23 0.6468881026352093 1102 24-25 0.7292868792185203 1102 26-27 0.8322670845078548 1102 28-29 0.8915155672262856 1102 30-31 0.8529669031909819 1102 32-33 1.040583798065363 1102 34-35 0.8571733695557455 1102 36-37 0.8793667361340951 1102 38-39 0.8972078577125515 1102 40-41 0.8647289705196002 1102 42-43 0.9151336660764571 1102 44-45 0.9945253253504447 1102 46-47 1.1018742643310944 1102 48-49 1.153485499098558 1102 50-51 1.0612804158190627 1102 52-53 1.0735940361643657 1102 54-55 1.1004711047662994 1102 56-57 1.091133415372326 1102 58-59 1.1848949577116876 1102 60-61 1.2354100062082694 1102 62-63 1.2816618257398105 1102 64-65 1.0817014798401487 1102 66-67 1.1344307523774724 1102 68-69 0.9926610484887952 1102 70-71 1.0307853389542458 1102 72-73 0.9709889758195693 1102 74-75 1.107031555132231 1102 76-77 1.0934460995468207 1102 78-79 1.0104147642407852 1102 80-81 0.942177303046801 1102 82-83 0.9557534866685344 1102 84-85 0.965639613781601 1102 86-87 0.8390744784786044 1102 88-89 0.8041599980991272 1102 90-91 0.8853068245588993 1102 92-93 0.8409478571813018 1102 94-95 0.7397721143382796 1102 96-97 0.5749282378126033 1102 98-99 0.6856345365921079 1102 100-101 0.7112007856527072 1102 102-103 0.4859903820253635 1102 104-105 0.6129946850620485 1102 106-107 0.7116354846233364 1102 108-109 0.7634675693347468 1102 110-111 0.8062164905138758 1102 112-113 0.619896375921666 1102 114-115 0.6843273389804345 1102 116-117 0.685496620391504 1102 118-119 0.598851383712173 1102 120-121 0.7803885097595327 1102 122-123 0.6384234603747281 1102 124-125 0.3911945861765105 1103 1 0.33801961362874877 1103 2 0.35755422148375615 1103 3 0.37393452760090895 1103 4 0.3828816810524529 1103 5 0.28604199057431856 1103 6 0.28735333935270546 1103 7 0.31351639881657434 1103 8 0.3348687185390844 1103 9 0.38491161472902036 1103 10-11 0.3796844719048522 1103 12-13 0.3703742053220296 1103 14-15 0.44045945042692125 1103 16-17 0.3316313451431725 1103 18-19 0.4350273386172745 1103 20-21 0.41553752085981444 1103 22-23 0.42333837147867115 1103 24-25 0.467438709794294 1103 26-27 0.6438831776688971 1103 28-29 0.7455861876733891 1103 30-31 0.5674719638987256 1103 32-33 0.675311558793652 1103 34-35 0.6403530066913916 1103 36-37 0.6739409288847469 1103 38-39 0.6901234007853319 1103 40-41 0.8906857034129345 1103 42-43 0.8862222093806338 1103 44-45 0.8337548595804023 1103 46-47 0.7543091933649322 1103 48-49 0.6915906972161352 1103 50-51 0.6863102512488339 1103 52-53 0.5704877538391173 1103 54-55 0.6881381846898194 1103 56-57 0.7155551358806918 1103 58-59 0.7984679881091772 1103 60-61 0.8329368841840079 1103 62-63 0.8034845542033651 1103 64-65 0.8456893061353945 1103 66-67 0.7995718431692787 1103 68-69 0.7389783523342679 1103 70-71 0.8032837405518123 1103 72-73 0.7714846898925813 1103 74-75 0.8000660273548554 1103 76-77 0.73771179365027 1103 78-79 0.7028767966466418 1103 80-81 0.6197936381675717 1103 82-83 0.5683327712516331 1103 84-85 0.6009387222316143 1103 86-87 0.6511326274430367 1103 88-89 0.6007855700384006 1103 90-91 0.6645283452381427 1103 92-93 0.5873090875161573 1103 94-95 0.6233602435564194 1103 96-97 0.6606718956830129 1103 98-99 0.6195164939807434 1103 100-101 0.6323295605247168 1103 102-103 0.47733219187681186 1103 104-105 0.46481273814539037 1103 106-107 0.5199288179534349 1103 108-109 0.5642650656704653 1103 110-111 0.664249838485329 1103 112-113 0.6404621636662 1103 114-115 0.5629947306364151 1103 116-117 0.5138082378194504 1103 118-119 0.4823093716047211 1103 120-121 0.493015045654694 1103 122-123 0.4034849981100699 1103 124-125 0.42353206635650054 1104 1 0.34409897541530654 1104 2 0.3313942551320359 1104 3 0.22958807594318742 1104 4 0.28658929597379057 1104 5 0.22502403499271395 1104 6 0.3042626460135551 1104 7 0.3974399964798465 1104 8 0.315901714025685 1104 9 0.3563585144912196 1104 10-11 0.3645386428735051 1104 12-13 0.3765374261169754 1104 14-15 0.38554298113620433 1104 16-17 0.33754881741141674 1104 18-19 0.3659425032759245 1104 20-21 0.4172697743904621 1104 22-23 0.3565685205645295 1104 24-25 0.483194509031307 1104 26-27 0.5440854860397621 1104 28-29 0.6635739806565439 1104 30-31 0.5708390517715429 1104 32-33 0.5343336818706064 1104 34-35 0.49932255433329686 1104 36-37 0.6221463936849005 1104 38-39 0.5908522366747988 1104 40-41 0.642834159312855 1104 42-43 0.726362495390994 1104 44-45 0.5768307223647042 1104 46-47 0.6265261448271033 1104 48-49 0.6681028000337008 1104 50-51 0.5962259901930054 1104 52-53 0.6343522766968164 1104 54-55 0.6850899394722774 1104 56-57 0.711343867323464 1104 58-59 0.7190225209681067 1104 60-61 0.6100695555585105 1104 62-63 0.4903139200341826 1104 64-65 0.47289438173459075 1104 66-67 0.613567983038017 1104 68-69 0.5879419144651088 1104 70-71 0.5458534755621045 1104 72-73 0.5528702619561372 1104 74-75 0.5192313078697808 1104 76-77 0.5851404980297801 1104 78-79 0.5239282149463165 1104 80-81 0.47934335930365535 1104 82-83 0.505989129643865 1104 84-85 0.4715748421740251 1104 86-87 0.40170810245376387 1104 88-89 0.3918231355435857 1104 90-91 0.3702375260034323 1104 92-93 0.30946837252830406 1104 94-95 0.33607200836865303 1104 96-97 0.29717628109282757 1104 98-99 0.19602118627884835 1104 100-101 0.18756909515836995 1104 102-103 -0.05802419344546195 1104 104-105 0.1716300473108312 1104 106-107 0.2444790957705969 1104 108-109 0.25013189661355995 1104 110-111 0.2632581254206414 1104 112-113 0.22285655365317325 1104 114-115 0.3068024268236762 1104 116-117 0.1762742422100949 1104 118-119 0.1783832117811066 1104 120-121 0.10722578132063632 1104 122-123 0.14318395973333153 1104 124-125 0.15220636367748597 1105 1 0.3471798050196817 1105 2 0.36602029391319846 1105 3 0.2964429662052801 1105 4 0.24431889324632294 1105 5 0.29143500168068925 1105 6 0.3262812552897856 1105 7 0.2535110952794781 1105 8 0.25683324325361667 1105 9 0.23755568758589618 1105 10-11 0.33535954634676557 1105 12-13 0.4594889851765629 1105 14-15 0.4682015506368842 1105 16-17 0.380298657598928 1105 18-19 0.26599754979911694 1105 20-21 0.30604028365958413 1105 22-23 0.3440765111888595 1105 24-25 0.5001479503268556 1105 26-27 0.5503980792637151 1105 28-29 0.6132241688801372 1105 30-31 0.45879274166429695 1105 32-33 0.2866642806347173 1105 34-35 0.45160520935140624 1105 36-37 0.5310799116904406 1105 38-39 0.5359522082636872 1105 40-41 0.5950679827378096 1105 42-43 0.6358154039782562 1105 44-45 0.6550500561242032 1105 46-47 0.6892969382073204 1105 48-49 0.6707485845354384 1105 50-51 0.5376591530596855 1105 52-53 0.6405405713200167 1105 54-55 0.6739252615832214 1105 56-57 0.7583820092369535 1105 58-59 0.7183433178997127 1105 60-61 0.7038217751763796 1105 62-63 0.5591709056155452 1105 64-65 0.5863523688075354 1105 66-67 0.4673972677883569 1105 68-69 0.5150629813022434 1105 70-71 0.5776384034689173 1105 72-73 0.5924815624746387 1105 74-75 0.667763696533747 1105 76-77 0.5041066888103742 1105 78-79 0.3825119211754533 1105 80-81 0.39222779711884215 1105 82-83 0.4399599372296308 1105 84-85 0.45596648930805017 1105 86-87 0.41871925381394703 1105 88-89 0.4954215800353836 1105 90-91 0.38512228344358945 1105 92-93 0.36440835406108363 1105 94-95 0.318696839677699 1105 96-97 0.2572231594222565 1105 98-99 0.2107106173463933 1105 100-101 0.33049472908123434 1105 102-103 0.16415067692885543 1105 104-105 0.3583132279344561 1105 106-107 0.2861368839657814 1105 108-109 0.43107958465958873 1105 110-111 0.3914676928615073 1105 112-113 0.4316870813895157 1105 114-115 0.5044416412565198 1105 116-117 0.4992685955022296 1105 118-119 0.4788351259795576 1105 120-121 0.4909533187877848 1105 122-123 0.5388663590514646 1105 124-125 0.49534596876307546 1106 1 0.21521495743005303 1106 2 0.22222955875306027 1106 3 0.22665477556068936 1106 4 0.22550592052978402 1106 5 0.1933893908801565 1106 6 0.29523313411621643 1106 7 0.2281297324182674 1106 8 0.3069962288185195 1106 9 0.3874431071488402 1106 10-11 0.36075214029706615 1106 12-13 0.3650647267429079 1106 14-15 0.3666909242296228 1106 16-17 0.39245114524023705 1106 18-19 0.4325752662401783 1106 20-21 0.45450360112563715 1106 22-23 0.4209692641713829 1106 24-25 0.5398601828696954 1106 26-27 0.565322175071195 1106 28-29 0.7146660610221502 1106 30-31 0.5789588809190178 1106 32-33 0.31454478544073083 1106 34-35 0.5517685543111668 1106 36-37 0.5236512201213017 1106 38-39 0.4325197178909761 1106 40-41 0.48748358334950126 1106 42-43 0.547880019578507 1106 44-45 0.5593701619300475 1106 46-47 0.47162787437699194 1106 48-49 0.46419784966281696 1106 50-51 0.4361031530524926 1106 52-53 0.5123118578277968 1106 54-55 0.4246447251504719 1106 56-57 0.5127614601175594 1106 58-59 0.7221607650821475 1106 60-61 0.5342857898684414 1106 62-63 0.6749041340055868 1106 64-65 0.6249003866550815 1106 66-67 0.5437268939638003 1106 68-69 0.4844766825121489 1106 70-71 0.4091954277514276 1106 72-73 0.6010573974836504 1106 74-75 0.49716861952441604 1106 76-77 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-3.18664607642987 2216 116-117 -3.063721686206133 2216 118-119 -3.1526358773090344 2216 120-121 -3.172779868027707 2216 122-123 -3.057038757346806 2216 124-125 -3.122538536813753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65152.0 3 13364.0 4 9393.0 5 7770.0 6 10405.0 7 9365.0 8 9478.0 9 10359.0 10 12548.0 11 14811.0 12 15791.0 13 16943.0 14 17037.0 15 16649.0 16 16565.0 17 16667.0 18 17066.0 19 17750.0 20 18510.0 21 19690.0 22 21573.0 23 28402.0 24 34012.0 25 30471.0 26 35777.0 27 38351.0 28 44396.0 29 54331.0 30 66046.0 31 81279.0 32 102209.0 33 123883.0 34 145791.0 35 173762.0 36 222276.0 37 294723.0 38 97174.0 39 272.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.975789446281205 16.964725483560255 9.491578371905344 23.567906698253196 2 11.822321066939878 24.1792469823381 34.89445862213657 29.103973328585454 3 23.670353968482 31.72880530171956 24.879241386059043 19.721599343739392 4 13.84130018025671 20.210613544290425 29.474757033421145 36.473329242031724 5 11.232800272343118 42.4831321124394 32.29884243763749 13.985225177579999 6 33.14017196081871 27.906751572993294 19.526719933535276 19.426356532652722 7 25.085149970124643 34.3522877028037 20.91014700340808 19.652415323663572 8 30.05281722221499 33.22388098553969 21.11095781650636 15.612343975738959 9 31.65476990717768 16.96133036821204 17.788072277174624 33.59582744743565 10-11 23.555009499142514 31.328554732218432 22.081776721073567 23.03465904756549 12-13 27.83342580281168 28.48061668767299 21.937240344533205 21.748717164982125 14-15 29.676346957893585 25.657955870865706 21.12109209573173 23.54460507550898 16-17 23.64526813777486 29.840555472405335 28.646992601076327 17.867183788743485 18-19 26.297571280632265 28.76288319041161 27.292232569288345 17.647312959667783 20-21 21.68665259569807 29.70783348989433 27.58045192119737 21.025061993210223 22-23 20.037466736296345 32.058686779438275 20.792867158135557 27.11097932612982 24-25 21.78538036416339 33.678345938562 19.009462250949557 25.52681144632505 26-27 24.667020359371225 27.819562573411194 20.716925393400697 26.79649167381688 28-29 24.016141472688115 27.37560760248578 25.02459304657802 23.583657878248086 30-31 27.02384587928116 30.08918675952691 22.69621698909228 20.190750372099647 32-33 19.28223651085645 30.673765973831628 26.01757922025989 24.026418295052025 34-35 16.80480723448909 32.42298269604884 27.3980918821558 23.37411818730627 36-37 25.093636954552057 29.78027256589455 23.23211296074924 21.893977518804153 38-39 27.902438136211533 27.425709481120602 22.430749102297156 22.24110328037071 40-41 21.98129973816304 31.862023081290662 22.59051867750358 23.566158503042715 42-43 26.251280081092826 29.82187721990187 21.83359895205675 22.09324374694855 44-45 28.87303383942859 26.663752390617073 22.097784369592652 22.365429400361684 46-47 23.18794267367745 29.23299995159832 29.287464850233242 18.291592524490987 48-49 25.95212191407217 28.750427078646744 27.29472967385373 18.002721333427356 50-51 20.635382352612126 30.31751685516333 27.58946902560465 21.457631766619894 52-53 19.208823331992615 32.312255997968634 21.1050082238083 27.37391244623045 54-55 21.082273078383025 33.45730400600887 19.35612780660887 26.10429510899923 56-57 24.301873540868684 27.855683377445978 20.85983885337478 26.982604228310564 58-59 23.74434497726285 27.01323491883379 25.084251416893572 24.15816868700979 60-61 26.484242194983747 29.721578897252837 23.06663863845322 20.7275402693102 62-63 19.08936159363412 30.354344710351345 26.04141327867134 24.5148804173432 64-65 16.60197211273557 31.822721136722947 27.546739842026984 24.02856690851449 66-67 24.559774919463564 29.463534465847403 23.453677045646245 22.52301356904279 68-69 27.164477413973387 27.05824746868064 22.78521751641517 22.992057600930806 70-71 21.78047812652464 30.966986501870224 22.81476662872012 24.437768742885023 72-73 25.82453324673757 29.12787030618681 22.226853846746312 22.820742600329314 74-75 28.303259562537942 26.14941324855794 22.46845448236363 23.07887270654049 76-77 22.93884362718977 28.549579947378938 29.299711083727438 19.211865341703856 78-79 25.38009562815229 28.256823353291853 27.47280050826847 18.89028051028739 80-81 20.498117637691895 29.77730908443739 27.602204246392887 22.122369031477824 82-83 19.14665981160907 31.4481664759063 21.46604391798805 27.93912979449658 84-85 20.91396151286365 32.572943275678774 19.959458467955745 26.55363674350183 86-87 23.826263166631886 27.02119931162213 21.662124489818222 27.490413031927762 88-89 23.463121178307368 26.390964083146294 25.398894757970414 24.74701998057592 90-91 25.790009371515275 29.15753668081847 23.46141417778613 21.591039769880126 92-93 19.24300366180274 29.64199783044758 25.992476560481414 25.12252194726827 94-95 17.046813761011716 30.725604319004564 27.753361505354913 24.474220414628807 96-97 24.4806526953257 28.461077662076807 24.021163800962885 23.03710584163461 98-99 26.56554695425169 26.661060173328018 23.23387008485932 23.53952278756097 100-101 21.657367074163655 29.75156214690955 23.34528407032723 25.245786708599567 102-103 25.35216870298492 28.51577866066045 22.817640817432302 23.314411818922327 104-105 27.37422207800852 25.849318174254527 23.01206091087593 23.764398836861023 106-107 22.528131787203236 28.04014695725258 29.181099225270657 20.250622030273526 108-109 24.64378176862752 28.355566767268677 27.241134041665095 19.759517422438712 110-111 19.9504588139144 29.772026267285177 27.618495931047075 22.659018987753342 112-113 18.946538017767946 31.22746884262809 22.023702387405336 27.802290752198626 114-115 20.3879737601936 32.98671701485658 20.507177035981808 26.118132188968012 116-117 22.955736652466847 27.850683715813236 21.98411107323452 27.2094685584854 118-119 22.75698348226797 27.20574057347967 25.179256307831384 24.858019636420977 120-121 24.705052056984755 29.841242330937106 23.52986640685016 21.923839205227974 122-123 19.22292585403868 30.39785688534628 25.406307923687304 24.972909336927735 124-125 17.629328035730726 31.650444579346736 26.61704585828289 24.10318152663965 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16705.0 1 11066.5 2 9436.5 3 12793.5 4 10413.5 5 7350.0 6 5065.5 7 3938.0 8 3649.5 9 3311.0 10 2598.5 11 1874.0 12 1581.5 13 1477.5 14 1391.0 15 1384.0 16 1413.0 17 1423.5 18 1443.5 19 1516.0 20 1658.0 21 1927.5 22 2328.0 23 2805.0 24 3148.5 25 3515.5 26 4271.0 27 5163.5 28 5853.5 29 6763.5 30 7997.0 31 9567.0 32 11220.5 33 12649.0 34 14279.0 35 16294.5 36 18772.5 37 21171.5 38 22816.5 39 23141.0 40 22317.0 41 22197.5 42 22979.5 43 24993.0 44 28113.0 45 31048.0 46 36933.0 47 50534.5 48 70589.0 49 132681.5 50 343333.5 51 363815.5 52 151303.0 53 63437.5 54 44493.5 55 36801.0 56 31477.0 57 27012.0 58 24534.5 59 22788.0 60 19732.5 61 16116.0 62 13104.5 63 10716.5 64 8337.0 65 6360.5 66 4693.0 67 3685.0 68 2679.5 69 1661.0 70 1169.0 71 879.5 72 670.0 73 527.0 74 421.0 75 323.5 76 222.0 77 150.0 78 108.5 79 65.0 80 46.5 81 37.5 82 27.0 83 18.0 84 15.0 85 13.0 86 8.0 87 8.0 88 8.0 89 5.0 90 3.0 91 4.0 92 4.5 93 3.0 94 2.5 95 5.0 96 5.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4865699744202325 2 0.47579300128857366 3 0.45869491891623027 4 0.46071560137841633 5 0.4629435333239035 6 0.46745120958570313 7 0.452062935450594 8 0.48009342806707217 9 0.49169939913193556 10-11 0.4976319155914305 12-13 0.49675110528740063 14-15 0.4720366044037407 16-17 0.4742645363492278 18-19 0.4603270085972267 20-21 0.4696532353457776 22-23 0.48724353524096126 24-25 0.47382413119721295 26-27 0.4846011043288718 28-29 0.48053383321908705 30-31 0.4932796764421067 32-33 0.49107765068203213 34-35 0.4845751981434591 36-37 0.44164864891471217 38-39 0.40276346461033724 40-41 0.3969604790779056 42-43 0.40411058625179463 44-45 0.4386435314068458 46-47 0.44662263651393935 48-49 0.44333255096653384 50-51 0.43006858403526144 52-53 0.42392881809246535 54-55 0.4256645325151124 56-57 0.408203763546992 58-59 0.4127632521796169 60-61 0.42320344490091144 62-63 0.4232811634571494 64-65 0.43765909636116535 66-67 0.4269339356003318 68-69 0.4283069634272018 70-71 0.4392911860421618 72-73 0.43879896851932165 74-75 0.4441097365289131 76-77 0.43403223040339556 78-79 0.42600131292547677 80-81 0.4345244479262358 82-83 0.4324001407223992 84-85 0.430457176816451 86-87 0.4363637870905333 88-89 0.42483553458190787 90-91 0.4279701830168374 92-93 0.4376331901757528 94-95 0.434938946892838 96-97 0.4039551491393188 98-99 0.37670184208521995 100-101 0.3775308400184245 102-103 0.3586193246671962 104-105 0.36108041228139715 106-107 0.3784893688786922 108-109 0.3859244440921203 110-111 0.4042401171788578 112-113 0.3991106924671548 114-115 0.3986702873151399 116-117 0.3693962977988551 118-119 0.443539800449835 120-121 0.4499904406175827 122-123 0.403721993470605 124-125 0.39698638526331825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1930041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.999169414351634 #Duplication Level Percentage of deduplicated Percentage of total 1 81.37426069264608 31.73528578720119 2 11.313265190209457 8.824158915649313 3 3.458838797052773 4.046755206695799 4 1.4928390797581732 2.3287793671941515 5 0.7532231665627531 1.4687538939797602 6 0.42235096926064924 0.9882802201507008 7 0.2571937183265603 0.7021238975327191 8 0.18571984804784736 0.5794335854100514 9 0.11987908169422928 0.42076661546071326 >10 0.5604220339628929 3.734703902451584 >50 0.03354671438130392 0.8960593821565097 >100 0.018924138085377754 1.5414854614651157 >500 0.0028311692222910924 0.7486599346306805 >1k 0.0025331514094183456 1.8236781858713045 >5k 0.0013410801579273595 3.910488575732963 >10k+ 0.0028311692222910924 36.25058706841748 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 125772 6.5165455034374915 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 103180 5.346000421752699 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 61308 3.176512830556449 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 38745 2.0074703076255895 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 37249 1.929959000870966 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 34408 1.7827600553563372 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 33871 1.75493681222316 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 29584 1.5328171784951719 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 25352 1.3135472251625744 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 22102 1.1451570199804046 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 15617 0.8091537951784444 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 14180 0.7346994183025127 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 12784 0.6623693486304176 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12717 0.6588979197851237 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 12540 0.6497271301490487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12473 0.6462557013037546 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 11142 0.577293435735303 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 10574 0.5478640091065423 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 10205 0.5287452442720129 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 9733 0.5042898052424793 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 8764 0.45408361791278007 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8708 0.45118212514656425 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8434 0.43698553554043673 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 7475 0.38729747191899033 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7137 0.3697848905800447 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 6397 0.33144373616933526 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 5373 0.2783878684442455 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 5271 0.27310300662006665 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 3778 0.19574713697791912 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 3161 0.16377890417871951 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 2645 0.1370437208328735 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2341 0.12129276010198746 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2286 0.1184430797065969 No Hit AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA 2035 0.10543817462945086 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.181237082528299E-5 0.0 3 0.0 0.0 0.0 5.181237082528299E-5 0.0 4 0.0 0.0 0.0 5.181237082528299E-5 0.0 5 0.0 0.0 0.0 5.181237082528299E-5 0.0 6 0.0 0.0 0.0 5.181237082528299E-5 0.0 7 0.0 0.0 0.0 5.181237082528299E-5 0.0 8 0.0 0.0 0.0 5.181237082528299E-5 0.0 9 0.0 0.0 0.0 1.5543711247584897E-4 0.0 10-11 2.5906185412641494E-5 0.0 0.0 4.922175228401883E-4 0.0 12-13 5.181237082528299E-5 0.0 0.0 0.0013471216414573577 0.0 14-15 5.181237082528299E-5 0.0 0.0 0.0027201494683273568 0.0 16-17 1.0362474165056598E-4 0.0 0.0 0.005284861824178864 0.0 18-19 1.0362474165056598E-4 0.0 0.0 0.007953198921680938 0.0 20-21 1.0362474165056598E-4 0.0 0.0 0.011657783435688672 0.0 22-23 1.0362474165056598E-4 0.0 0.0 0.01823795453049961 0.0 24-25 1.0362474165056598E-4 0.0 0.0 0.02430000191705772 0.0 26-27 1.0362474165056598E-4 0.0 0.0 0.03069882971398017 0.0 28-29 1.0362474165056598E-4 0.0 0.0 0.040102775018769035 0.0 30-31 1.0362474165056598E-4 0.0 0.0 0.05264136875848752 0.0 32-33 1.0362474165056598E-4 0.0 0.0 0.07295181812199844 0.0 34-35 1.2953092706320747E-4 0.0 0.0 0.10036056228857315 0.0 36-37 1.5543711247584897E-4 0.0 0.0 0.13719915794534937 0.0 38-39 2.0724948330113195E-4 0.0 0.0 0.19489223285930196 0.0 40-41 2.0724948330113195E-4 0.0 0.0 0.2991905353305966 0.0 42-43 2.0724948330113195E-4 0.0 0.0 0.3761319060061419 0.0 44-45 2.5906185412641493E-4 0.0 0.0 0.44369523756231083 0.0 46-47 2.5906185412641493E-4 0.0 0.0 0.5351181658835227 0.0 48-49 2.5906185412641493E-4 0.0 0.0 0.6130957839755735 0.0 50-51 2.5906185412641493E-4 0.0 0.0 0.6993892875850823 0.0 52-53 2.5906185412641493E-4 0.0 0.0 0.8216923889181629 0.0 54-55 2.5906185412641493E-4 0.0 0.0 0.936871289262767 0.0 56-57 2.5906185412641493E-4 0.0 0.0 1.0569982710211856 0.0 58-59 2.5906185412641493E-4 0.0 0.0 1.2092748288766924 0.0 60-61 2.5906185412641493E-4 0.0 0.0 1.3510593816400793 0.0 62-63 3.1087422495169794E-4 0.0 0.0 1.484320799402707 0.0 64-65 3.1087422495169794E-4 0.0 0.0 1.6458717716359392 0.0 66-67 3.1087422495169794E-4 0.0 0.0 1.8207903355420947 0.0 68-69 3.1087422495169794E-4 0.0 0.0 2.0542309722954073 0.0 70-71 3.1087422495169794E-4 0.0 0.0 2.4437563761598846 0.0 72-73 3.1087422495169794E-4 0.0 0.0 2.723698615728889 0.0 74-75 3.1087422495169794E-4 0.0 0.0 2.966569103972403 0.0 76-77 3.1087422495169794E-4 0.0 0.0 3.2500604909429383 0.0 78-79 3.1087422495169794E-4 0.0 0.0 3.511687057425205 0.0 80-81 4.6631133742754686E-4 0.0 0.0 3.799271621690938 0.0 82-83 4.6631133742754686E-4 0.0 0.0 4.204677517213365 0.0 84-85 4.6631133742754686E-4 0.0 0.0 4.570887354206465 0.0 86-87 4.6631133742754686E-4 0.0 0.0 4.921967978918582 0.0 88-89 4.6631133742754686E-4 0.0 0.0 5.347502980506631 0.0 90-91 4.6631133742754686E-4 0.0 0.0 5.730059620495108 0.0 92-93 4.6631133742754686E-4 0.0 0.0 6.109533424419482 0.0 94-95 4.922175228401883E-4 0.0 0.0 6.564575571192529 0.0 96-97 5.181237082528299E-4 0.0 0.0 7.006923687113383 0.0 98-99 5.181237082528299E-4 0.0 0.0 7.54061701279921 0.0 100-101 5.181237082528299E-4 0.0 0.0 8.401427741690462 0.0 102-103 5.181237082528299E-4 0.0 0.0 9.055714360472136 0.0 104-105 5.699360790781128E-4 0.0 0.0 9.621090950917623 0.0 106-107 5.699360790781128E-4 0.0 0.0 10.225741318448675 0.0 108-109 5.699360790781128E-4 0.0 0.0 10.736766731898442 0.0 110-111 5.699360790781128E-4 0.0 0.0 11.283283619363527 0.0 112-113 5.699360790781128E-4 0.0 0.0 12.035936024157 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 2380 0.0 49.28432 5 GAACAAA 2055 0.0 45.770496 1 AAGTACT 2575 0.0 44.16359 4 AAAAAGT 2640 0.0 43.29499 1 AAAAGTA 2755 0.0 40.62555 2 CGCAAAA 495 0.0 37.28374 2 AAAGTAC 3065 0.0 36.325203 3 GTACAAA 1560 0.0 33.96298 1 TATAACG 115 2.7542485E-5 31.06107 2 GTACAAG 1025 0.0 30.781683 1 AGAACAA 1770 0.0 27.58059 2 GGGCGTA 295 7.2759576E-12 26.231096 9 ATAGGGG 150 1.679811E-4 23.815348 3 TACAAAA 1840 0.0 23.295801 2 ATGATCG 130 0.002047769 22.894005 8 GTACATA 850 0.0 21.711164 1 GACTAGC 200 4.8919006E-5 20.835716 1 GTCCAAG 700 0.0 20.410498 1 ACGATCG 205 2.5465852E-11 20.31483 82-83 AGGGCGT 390 2.728484E-10 19.84147 8 >>END_MODULE