##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364139_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1930041 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09891240652401 33.0 31.0 34.0 30.0 34.0 2 31.91795355642704 33.0 31.0 34.0 30.0 34.0 3 32.15861113831261 34.0 31.0 34.0 30.0 34.0 4 35.58643728293855 37.0 35.0 37.0 33.0 37.0 5 35.71534076219106 37.0 35.0 37.0 33.0 37.0 6 35.88570968181505 37.0 35.0 37.0 35.0 37.0 7 35.85837295684392 37.0 35.0 37.0 35.0 37.0 8 35.92629275751137 37.0 35.0 37.0 35.0 37.0 9 37.592535080860976 39.0 37.0 39.0 35.0 39.0 10-11 37.55310586666293 39.0 37.0 39.0 35.0 39.0 12-13 37.53834996251375 39.0 37.0 39.0 35.0 39.0 14-15 38.81621219445597 40.0 38.0 41.0 34.0 41.0 16-17 38.77414987557259 40.0 38.0 41.0 34.0 41.0 18-19 38.81445420071387 40.0 38.0 41.0 35.0 41.0 20-21 38.87787098823289 40.0 38.0 41.0 35.0 41.0 22-23 38.872543122140925 40.0 38.0 41.0 35.0 41.0 24-25 38.84504992380991 40.0 38.0 41.0 35.0 41.0 26-27 38.62023501055159 40.0 38.0 41.0 34.0 41.0 28-29 38.450016605864846 40.0 38.0 41.0 34.0 41.0 30-31 38.290867396081225 40.0 38.0 41.0 34.0 41.0 32-33 37.95730608831626 40.0 37.0 41.0 34.0 41.0 34-35 37.790376991991366 40.0 37.0 41.0 33.0 41.0 36-37 37.72648456690817 40.0 37.0 41.0 33.0 41.0 38-39 37.62635897372128 40.0 37.0 41.0 33.0 41.0 40-41 37.338831921187165 40.0 36.0 41.0 33.0 41.0 42-43 37.14604922900601 40.0 36.0 41.0 31.5 41.0 44-45 36.895433309447824 40.0 35.5 41.0 31.0 41.0 46-47 36.53134311654519 39.0 35.0 41.0 30.5 41.0 48-49 36.17848221877152 39.0 35.0 41.0 30.0 41.0 50-51 36.060057014332855 39.0 35.0 41.0 29.0 41.0 52-53 35.99128671359831 39.0 35.0 41.0 29.0 41.0 54-55 35.782030019051405 39.0 35.0 41.0 28.0 41.0 56-57 35.43885855274577 38.5 35.0 40.0 28.0 41.0 58-59 35.23445253235553 38.0 35.0 40.0 27.5 41.0 60-61 35.01253367156448 38.0 35.0 40.0 28.0 41.0 62-63 34.609443270894246 37.5 34.0 40.0 26.5 41.0 64-65 34.320138794978966 37.0 34.0 39.0 26.0 41.0 66-67 33.80048454929196 36.0 34.0 39.0 26.0 41.0 68-69 33.47456504810001 36.0 33.5 39.0 25.5 40.5 70-71 32.92321665705548 35.0 33.0 38.0 24.5 39.5 72-73 32.53557411474679 35.0 33.0 37.0 24.5 39.0 74-75 32.18474814783727 35.0 32.0 37.0 23.5 39.0 76-77 31.580038455141626 35.0 32.0 36.0 21.5 37.5 78-79 31.160885701391837 35.0 31.5 35.0 20.5 37.0 80-81 31.00014377932904 35.0 31.5 35.0 20.0 36.5 82-83 30.86228297740825 35.0 32.0 35.0 20.0 36.0 84-85 30.6149400453151 34.5 31.0 35.0 20.0 36.0 86-87 30.323273184352043 34.0 31.0 35.0 18.0 35.0 88-89 30.11100360044165 34.0 31.0 35.0 15.5 35.0 90-91 29.909443892642695 34.0 31.0 35.0 10.5 35.0 92-93 29.66951712424762 34.0 30.0 35.0 7.0 35.0 94-95 29.631141514610313 34.0 30.5 35.0 7.0 35.0 96-97 29.52950222300977 34.0 30.0 35.0 4.0 35.0 98-99 29.406740582194885 34.0 30.0 35.0 2.0 35.0 100-101 29.099907204043852 34.0 29.0 35.0 2.0 35.0 102-103 27.494052717014817 32.5 26.0 34.0 2.0 35.0 104-105 28.370537724328138 34.0 29.0 35.0 2.0 35.0 106-107 28.38940494010231 34.0 29.0 35.0 2.0 35.0 108-109 28.32813862503439 34.0 29.0 35.0 2.0 35.0 110-111 28.38740783226885 34.0 29.0 35.0 2.0 35.0 112-113 28.379214483008393 34.0 29.0 35.0 2.0 35.0 114-115 28.218136816782646 34.0 29.0 35.0 2.0 35.0 116-117 27.896979649655112 34.0 29.0 35.0 2.0 35.0 118-119 27.64688081755776 34.0 27.0 35.0 2.0 35.0 120-121 27.35971334287717 34.0 27.0 35.0 2.0 35.0 122-123 26.94023987055197 34.0 26.0 35.0 2.0 35.0 124-125 25.79620562464735 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2159086726148871 1101 2 0.5333546935208773 1101 3 0.3958178253762412 1101 4 0.4691526286537737 1101 5 0.20546587915414705 1101 6 0.21009867598603194 1101 7 0.2306139896551258 1101 8 0.19269428250086662 1101 9 0.31033784794677644 1101 10-11 0.30973134092879917 1101 12-13 0.2742243118786192 1101 14-15 0.3352246545557449 1101 16-17 0.3964976555905295 1101 18-19 0.2868362067896939 1101 20-21 0.19940109555104613 1101 22-23 0.2867077365983235 1101 24-25 0.31690286794378153 1101 26-27 0.24861217428423288 1101 28-29 0.35349274975141043 1101 30-31 0.34555402929780143 1101 32-33 0.3650477105227097 1101 34-35 0.29964060758629074 1101 36-37 0.42487150442426014 1101 38-39 0.4053711631555714 1101 40-41 0.5371462172203181 1101 42-43 0.6344609853970482 1101 44-45 0.6061560903205176 1101 46-47 0.5851396314334352 1101 48-49 0.558505486045874 1101 50-51 0.5489672477877221 1101 52-53 0.5143298236554585 1101 54-55 0.6716422792838301 1101 56-57 0.6242879182281129 1101 58-59 0.6385031099917384 1101 60-61 0.49533796271567354 1101 62-63 0.5514148435955946 1101 64-65 0.49204036680657737 1101 66-67 0.5310890119016207 1101 68-69 0.42802627939859406 1101 70-71 0.45676912102366884 1101 72-73 0.5222469160987089 1101 74-75 0.5396957998187304 1101 76-77 0.6391781866999509 1101 78-79 0.5811370578593191 1101 80-81 0.5613582469155709 1101 82-83 0.5949848880726236 1101 84-85 0.6541296183837062 1101 86-87 0.7199819255950004 1101 88-89 0.5917900540486194 1101 90-91 0.6711220205989683 1101 92-93 0.7249172395651975 1101 94-95 0.679602654017728 1101 96-97 0.6792375860917303 1101 98-99 0.6496343631199508 1101 100-101 0.6789786577142713 1101 102-103 0.5579253984790569 1101 104-105 0.7301872519296957 1101 106-107 0.7112155169852947 1101 108-109 0.7120472712084194 1101 110-111 0.6690485235365671 1101 112-113 0.7663799762952337 1101 114-115 0.8994151055294921 1101 116-117 0.8544758796971408 1101 118-119 0.8688303136686706 1101 120-121 0.8539063061878451 1101 122-123 0.8951257756118878 1101 124-125 0.8922259970894579 1102 1 0.27636372912644447 1102 2 0.5832181116905772 1102 3 0.4376675926677436 1102 4 0.4926629284082722 1102 5 0.27242657159300876 1102 6 0.2237193443585639 1102 7 0.2285644264780302 1102 8 0.18594189174621079 1102 9 0.2510993558775425 1102 10-11 0.23916267938616187 1102 12-13 0.2869617143513423 1102 14-15 0.3695483996130662 1102 16-17 0.40768513317304667 1102 18-19 0.36638096208579185 1102 20-21 0.29884855293012436 1102 22-23 0.29464939152463643 1102 24-25 0.3571720671841234 1102 26-27 0.35013426651754287 1102 28-29 0.3933164160031879 1102 30-31 0.4083942380278671 1102 32-33 0.36947097342381596 1102 34-35 0.42407945083234466 1102 36-37 0.3889252194710977 1102 38-39 0.41898508384215916 1102 40-41 0.49078255755775047 1102 42-43 0.503362114216813 1102 44-45 0.5318937826342065 1102 46-47 0.6262356388709946 1102 48-49 0.6527855160031208 1102 50-51 0.5312296862181896 1102 52-53 0.48378564940321667 1102 54-55 0.4912083523150841 1102 56-57 0.5637484846895404 1102 58-59 0.611737454153193 1102 60-61 0.6175126168472431 1102 62-63 0.5945239519439909 1102 64-65 0.5586220126303232 1102 66-67 0.5724340646654298 1102 68-69 0.5546155296786921 1102 70-71 0.46349965918072655 1102 72-73 0.4259415898716128 1102 74-75 0.45793458445277224 1102 76-77 0.5026728095371169 1102 78-79 0.4618887327402099 1102 80-81 0.502294389911043 1102 82-83 0.5058686950254163 1102 84-85 0.49121577939983396 1102 86-87 0.4970224722541303 1102 88-89 0.41745071131284917 1102 90-91 0.4455995608953458 1102 92-93 0.43711803386068837 1102 94-95 0.4963992662584289 1102 96-97 0.5254560900294649 1102 98-99 0.5353782113041952 1102 100-101 0.5924555570423884 1102 102-103 0.4434830971987189 1102 104-105 0.3126332026557428 1102 106-107 0.22319711672667708 1102 108-109 0.23774685457285827 1102 110-111 0.35897285411969193 1102 112-113 0.43694593876348975 1102 114-115 0.35429976776695327 1102 116-117 0.3545727616100365 1102 118-119 0.29096981792794807 1102 120-121 0.3723227221067873 1102 122-123 0.2966831043850249 1102 124-125 0.5484933035110373 1103 1 0.2753915218531162 1103 2 0.5832364346184633 1103 3 0.3979156972458 1103 4 0.5055296281371042 1103 5 0.24317844626515495 1103 6 0.2136200134609254 1103 7 0.21453913525571977 1103 8 0.15366878450163313 1103 9 0.2682536207331552 1103 10-11 0.21193978363790933 1103 12-13 0.25254391446416236 1103 14-15 0.449901112334679 1103 16-17 0.425182558558987 1103 18-19 0.2831965682695241 1103 20-21 0.26437293267778017 1103 22-23 0.2946087413203742 1103 24-25 0.24800867387009617 1103 26-27 0.2200414784826421 1103 28-29 0.39124974748400376 1103 30-31 0.39486172435636036 1103 32-33 0.3475858741990905 1103 34-35 0.46503817194537334 1103 36-37 0.4190832419830528 1103 38-39 0.38484746700467554 1103 40-41 0.48147681572246626 1103 42-43 0.5215846300373315 1103 44-45 0.4512221781609398 1103 46-47 0.48420770817632075 1103 48-49 0.40097461007509594 1103 50-51 0.49308941971544584 1103 52-53 0.40175202039930014 1103 54-55 0.3950408293043495 1103 56-57 0.4590284620613332 1103 58-59 0.36443089421738506 1103 60-61 0.37901064846727905 1103 62-63 0.3188576253233606 1103 64-65 0.32437581727709386 1103 66-67 0.3316609026340842 1103 68-69 0.3747690151424763 1103 70-71 0.404660490682474 1103 72-73 0.41258672365992766 1103 74-75 0.4267078862722897 1103 76-77 0.221123002904676 1103 78-79 0.23524430760022952 1103 80-81 0.331012024006057 1103 82-83 0.2675856917009547 1103 84-85 0.16659216692174894 1103 86-87 0.20398455386493808 1103 88-89 0.19186768120779263 1103 90-91 0.1374462584181444 1103 92-93 0.13594522086291505 1103 94-95 0.21875867449338315 1103 96-97 0.21191323785824068 1103 98-99 0.08687291931499175 1103 100-101 0.24324773244838127 1103 102-103 0.08587888190601234 1103 104-105 0.29580887184608073 1103 106-107 0.09441162712651519 1103 108-109 0.09492381801716121 1103 110-111 0.10856953735424568 1103 112-113 0.29093277125225114 1103 114-115 0.24037647822137131 1103 116-117 0.21316380611521524 1103 118-119 0.28650211779151746 1103 120-121 0.25536255894293447 1103 122-123 0.246584033163316 1103 124-125 0.24591921123313298 1104 1 0.1901565120374471 1104 2 0.45988899235390335 1104 3 0.08942957002371088 1104 4 0.34848846894630015 1104 5 0.14912301510376835 1104 6 0.15778811306591933 1104 7 0.12206263243091797 1104 8 0.0730307946817561 1104 9 0.29153978049967577 1104 10-11 0.18799399247991033 1104 12-13 0.255965599904151 1104 14-15 0.2443868888952565 1104 16-17 0.33550843347607895 1104 18-19 0.23202960771965309 1104 20-21 0.11154143623339507 1104 22-23 0.0821245258457779 1104 24-25 0.09499601817781667 1104 26-27 0.242248592866801 1104 28-29 0.3318447396381359 1104 30-31 0.23270427353698864 1104 32-33 0.27901335197704924 1104 34-35 0.2589074361081458 1104 36-37 0.27149763204615596 1104 38-39 0.18180601377206784 1104 40-41 0.3579870510415475 1104 42-43 0.3209526066966859 1104 44-45 0.3143574369129638 1104 46-47 0.43977325808682366 1104 48-49 0.3094067582645508 1104 50-51 0.24712303801873503 1104 52-53 0.27761315130880604 1104 54-55 0.24227618216878 1104 56-57 0.4363165283415853 1104 58-59 0.363683716400125 1104 60-61 0.35555617593924893 1104 62-63 0.4038297579638126 1104 64-65 0.3790848396023847 1104 66-67 0.33974592210839916 1104 68-69 0.4549622548455474 1104 70-71 0.39731628525668583 1104 72-73 0.35093322384881986 1104 74-75 0.4046197803106679 1104 76-77 0.419257752555211 1104 78-79 0.3565198376129537 1104 80-81 0.23668943350731908 1104 82-83 0.2399511747145695 1104 84-85 0.26472546539510944 1104 86-87 0.26857543847399157 1104 88-89 0.34717147206319154 1104 90-91 0.3576366409674776 1104 92-93 0.30030102666590963 1104 94-95 0.30536926941892517 1104 96-97 0.29645364529169527 1104 98-99 0.27825547245424787 1104 100-101 0.2878719061100412 1104 102-103 0.23399904841551233 1104 104-105 0.17065947196731202 1104 106-107 0.15739253772353834 1104 108-109 0.16354023240104 1104 110-111 0.1158085919099463 1104 112-113 0.07023875885681719 1104 114-115 0.10162728826371037 1104 116-117 -0.14215751781512154 1104 118-119 -0.0807918340826852 1104 120-121 -0.11232214022566112 1104 122-123 -0.12468673889148363 1104 124-125 -0.015767047118274036 1105 1 0.16764070835821343 1105 2 0.43069657811994233 1105 3 0.18227174631464038 1105 4 0.41690597018928344 1105 5 0.22308956102322242 1105 6 0.1008149615636853 1105 7 0.15197420285495866 1105 8 0.09534239351690132 1105 9 0.18132243551778515 1105 10-11 0.17411250231726427 1105 12-13 0.18460932460050827 1105 14-15 0.2663921804642655 1105 16-17 0.344861903182732 1105 18-19 0.15236308310853985 1105 20-21 0.10905990345408156 1105 22-23 0.15189155475408 1105 24-25 0.22270839121568287 1105 26-27 0.2007284209795941 1105 28-29 0.34656524535586897 1105 30-31 0.28291646900541423 1105 32-33 0.1769730792304074 1105 34-35 0.20566768186423445 1105 36-37 0.2305900746912286 1105 38-39 0.11378804374579232 1105 40-41 0.1582258819244231 1105 42-43 0.21657729595018793 1105 44-45 0.2669685953759213 1105 46-47 0.12634545781691742 1105 48-49 0.2007786596780079 1105 50-51 0.18676274964598605 1105 52-53 0.24537098212065445 1105 54-55 0.3499676020139333 1105 56-57 0.3025401592812855 1105 58-59 0.3913534727748669 1105 60-61 0.4263619860109884 1105 62-63 0.35727993198185004 1105 64-65 0.4214173916302215 1105 66-67 0.3716329524372597 1105 68-69 0.23459859265362581 1105 70-71 0.28379170964762324 1105 72-73 0.3147889664441692 1105 74-75 0.32227195731186953 1105 76-77 0.18504811408656607 1105 78-79 0.21024258070550061 1105 80-81 0.1396122263193078 1105 82-83 0.1718843730951356 1105 84-85 0.20750815592710836 1105 86-87 0.16208261227895093 1105 88-89 0.07897060552437196 1105 90-91 0.087495828409768 1105 92-93 0.09496861669360968 1105 94-95 0.17820736161297646 1105 96-97 0.08241589403095162 1105 98-99 0.12051166962734072 1105 100-101 0.09341562370362055 1105 102-103 -0.10171815451083432 1105 104-105 0.09213827017741139 1105 106-107 0.06538049851620542 1105 108-109 0.04154115576207573 1105 110-111 0.12923728002699875 1105 112-113 0.2290790529117963 1105 114-115 0.39618833663362096 1105 116-117 0.2795166067675865 1105 118-119 0.23945907152140578 1105 120-121 0.2844256756703558 1105 122-123 0.16556381157374744 1105 124-125 0.2293764289808813 1106 1 0.20290498037698512 1106 2 0.5018109357645386 1106 3 0.3733026884143342 1106 4 0.5135297251107502 1106 5 0.19409583066639158 1106 6 0.15032115303847604 1106 7 0.1833156800756086 1106 8 0.1018838290477575 1106 9 0.1717548953419339 1106 10-11 0.14098303571312698 1106 12-13 0.20849380790751582 1106 14-15 0.27773692817806506 1106 16-17 0.23150652097529445 1106 18-19 0.09976182810171252 1106 20-21 0.0780719809180539 1106 22-23 0.14178874087209437 1106 24-25 0.15862424609922954 1106 26-27 0.13768786866158678 1106 28-29 0.2121151823719245 1106 30-31 0.24307710053405174 1106 32-33 0.2977607469554968 1106 34-35 0.19887736539909895 1106 36-37 0.2552167083654453 1106 38-39 0.2035918321269108 1106 40-41 0.2395367207340371 1106 42-43 0.22697818293668348 1106 44-45 0.29365950322529244 1106 46-47 0.40356714693677276 1106 48-49 0.3277991326056906 1106 50-51 0.3170941476380449 1106 52-53 0.3383758555148333 1106 54-55 0.34261999890804873 1106 56-57 0.45983987302328444 1106 58-59 0.39405342659262743 1106 60-61 0.3896630546715798 1106 62-63 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120-121 -2.291685176200346 2216 122-123 -2.486130008846793 2216 124-125 -2.799863924757357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38.0 3 11.0 4 60.0 5 233.0 6 662.0 7 1148.0 8 2062.0 9 3916.0 10 7214.0 11 9490.0 12 9972.0 13 12814.0 14 17450.0 15 17359.0 16 11559.0 17 11431.0 18 11722.0 19 11726.0 20 12110.0 21 12087.0 22 13051.0 23 14041.0 24 15653.0 25 18046.0 26 21334.0 27 26019.0 28 31574.0 29 38945.0 30 48238.0 31 60489.0 32 78007.0 33 103398.0 34 148187.0 35 240418.0 36 349171.0 37 442782.0 38 127295.0 39 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.2630124376212 17.42818491887188 11.155148803639804 24.153653839867122 2 11.838374082983902 23.485819183777306 34.570010833989194 30.1057958992496 3 23.806846825935104 28.417597004818052 27.95216454848181 19.823391620765033 4 14.225863595643823 19.80761030465156 30.91784060545864 35.04868549424598 5 11.188052481786656 38.619594091524476 35.14821705860135 15.044136368087518 6 34.069462579535326 27.57383572716533 18.264989947978197 20.09171174532115 7 22.34128705037872 33.06261369577123 23.22567240799548 21.37042684585457 8 29.253471817438076 31.32643296178682 22.671798163873202 16.7482970569019 9 29.606262250387427 14.171926917614702 20.544537654899557 35.677273177098314 10-11 23.941751481380834 27.30157735031985 24.393477753446106 24.363193414853214 12-13 28.714590165378457 25.274892392860494 24.80689784174302 21.20361960001803 14-15 29.894050293274237 21.42198563278765 25.111507940722877 23.572456133215233 16-17 22.71846340331751 26.28726525966027 32.30297018468352 18.691301152338706 18-19 25.4951060625137 23.96037182629799 32.625964940641154 17.918557170547153 20-21 20.912008422636458 24.40372904768147 33.24223836123349 21.442024168448583 22-23 19.373531280820274 26.532733380465334 27.35491819990306 26.73881713881134 24-25 21.75684350746953 28.015259779455466 25.311431207938067 24.916465505136937 26-27 23.995811487942483 22.754283458227054 27.342320707176686 25.907584346653778 28-29 23.016312394253887 21.37794874757485 31.993064376277015 23.612674481894246 30-31 25.81134286784581 24.24044359679406 29.535693801323394 20.41251973403674 32-33 18.835428884671362 24.59079884831462 32.16068985063011 24.4130824163839 34-35 16.556370996552143 26.45409588767484 34.09748867834052 22.8920444374325 36-37 24.48095662216502 24.396528363905222 28.970317210877905 22.152197803051852 38-39 26.173148653318762 22.276961991999134 29.099666794643227 22.45022256003888 40-41 21.662265636394675 25.16296937810372 29.292649558390092 23.882115427111504 42-43 25.605932723708978 24.189719285756105 28.36665127839253 21.837696712142385 44-45 28.239503720387287 20.605546721546332 28.362480382541094 22.792469175525287 46-47 22.578924489168884 24.019412022853402 34.80451969673183 18.597143791245884 48-49 25.573031868234924 23.746775327570763 32.383042640026815 18.297150164167498 50-51 20.507699059242782 24.963252075992166 32.22478175334099 22.30426711142406 52-53 19.15912667140232 26.904013956180208 26.396200909721607 27.540658462695873 54-55 21.43879326915853 28.000337816657783 24.472433487164263 26.088435427019423 56-57 24.01138110537548 23.067463333680475 26.202992578914124 26.718162982029913 58-59 23.163782531044678 21.483610969922402 30.781030040294482 24.57157645873844 60-61 25.77269083921015 24.399507575227677 28.48719275911755 21.340608826444623 62-63 19.058584765815855 24.73271293200507 31.01662089043704 25.19208141174203 64-65 16.76909972378825 26.334984593591535 33.03911678560196 23.85679889701825 66-67 24.29774807892682 24.664450133442763 28.011840162980995 23.025961624649423 68-69 25.83522837079627 22.482527573248447 28.27750809438763 23.404735961567656 70-71 21.707155443848084 25.1909933519547 28.36501918871154 24.736832015485682 72-73 25.360782491149152 24.313784007697244 27.635086508524946 22.690346992628655 74-75 27.672624571187864 20.887794611617057 27.830393240350855 23.609187576844224 76-77 22.28165101155882 24.010940700223465 34.16207220468374 19.545336083533975 78-79 24.78527658220732 23.813172880783362 32.078411805759565 19.32313873124975 80-81 20.24433677833787 24.865352601317795 31.926264778831126 22.96404584151321 82-83 18.939442219103118 26.530446762529913 26.428894515712358 28.10121650265461 84-85 21.08336040529709 27.4026044006319 24.78348905541385 26.730546138657157 86-87 23.346472950574626 22.58498653655544 26.60707725898051 27.461463253889423 88-89 22.5777328046399 21.37125584378777 30.587769897116175 25.46324145445615 90-91 24.83245174584374 24.24046950297947 28.41509584511417 22.511982906062617 92-93 18.791647431323998 24.555022406259763 30.399742803391224 26.253587359025016 94-95 16.691355453242597 25.83327892992115 32.55495003694251 24.920415579893742 96-97 23.725712183545944 24.114472688836152 28.03070498139877 24.129110146219134 98-99 24.919156481238243 22.403486491709945 28.08286998248153 24.594487044570286 100-101 21.141545697733882 24.57546238655034 28.342895306369137 25.94009660934664 102-103 24.509148112319366 23.704390917743932 27.82579544353487 23.96066552640183 104-105 26.401081260925196 20.56611244097867 28.072231588013885 24.960574710082252 106-107 21.421753153877727 23.503177715775813 33.7486940894072 21.32637504093926 108-109 23.878291665932508 23.72989657170279 31.3502839380902 21.041527824274503 110-111 19.42016691104702 24.528083357581355 31.62305952110433 24.42869021026729 112-113 18.25397545121789 25.96795594454099 26.77782789942425 29.00024070481687 114-115 20.155393159622037 26.98986204639092 25.126325946210297 27.728418847776748 116-117 22.13784595608149 22.532091403158212 26.708807285713913 28.621255355046376 118-119 21.469888528046265 21.437394553294652 30.22297241095705 26.86974450770203 120-121 23.52143607420815 23.98105257594075 28.27554811191645 24.221963237934645 122-123 18.226536026280066 24.480435030414824 29.760697002041468 27.53233194126364 124-125 16.418179180418754 25.78463324024228 31.49991450821498 26.297273071123982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8243.0 1 5399.5 2 4226.5 3 5110.0 4 2726.0 5 1541.0 6 3444.5 7 8024.5 8 12099.0 9 12072.5 10 8162.0 11 3778.0 12 2198.5 13 2024.0 14 2180.5 15 2665.0 16 2792.0 17 2990.5 18 3259.0 19 3060.0 20 2937.0 21 3157.0 22 3535.0 23 3899.0 24 4148.0 25 4403.5 26 4887.0 27 5514.0 28 5824.0 29 6464.0 30 7621.0 31 8953.5 32 10684.0 33 12134.0 34 13395.0 35 15046.0 36 17716.5 37 20251.5 38 21464.0 39 21453.5 40 20675.5 41 20567.5 42 21145.5 43 22577.0 44 25254.5 45 28351.5 46 33976.5 47 49010.5 48 69811.0 49 140325.0 50 387282.5 51 389236.0 52 131992.0 53 49666.0 54 37306.0 55 32246.0 56 28443.0 57 25104.0 58 23464.0 59 21820.5 60 18845.0 61 15332.0 62 12324.0 63 10167.0 64 7922.0 65 6067.0 66 4329.5 67 3362.5 68 2468.5 69 1524.0 70 1081.5 71 769.0 72 589.5 73 463.0 74 356.0 75 280.0 76 184.5 77 118.5 78 92.0 79 55.5 80 33.0 81 22.5 82 14.0 83 5.5 84 2.0 85 7.0 86 6.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15046312487662178 2 2.0724948330113195E-4 3 4.144989666022639E-4 4 0.0 5 0.0 6 0.004196802036847922 7 0.0 8 0.0 9 0.0 10-11 2.5906185412641494E-5 12-13 0.005388486565829431 14-15 0.0011139659727435842 16-17 4.922175228401883E-4 18-19 0.0 20-21 2.0724948330113195E-4 22-23 2.5906185412641494E-5 24-25 0.0 26-27 0.0 28-29 2.849680395390564E-4 30-31 0.0 32-33 0.0 34-35 7.771855623792449E-5 36-37 0.0 38-39 0.0 40-41 2.5906185412641494E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.771855623792449E-4 96-97 0.003160554620342262 98-99 0.004041364924372073 100-101 0.0 102-103 0.004144989666022639 104-105 0.02290106790477508 106-107 0.018289766901324896 108-109 0.02046588647598678 110-111 0.017123988557756025 112-113 0.01510330609556999 114-115 0.041734864699765443 116-117 0.040724523468672426 118-119 0.02344509779844055 120-121 0.014507463831079235 122-123 0.003160554620342262 124-125 0.0016061834955837725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1930041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.34425382863757 #Duplication Level Percentage of deduplicated Percentage of total 1 80.48638275091332 34.0813582095355 2 11.857901847721337 10.042280114299654 3 3.6636565293419734 4.6540440605820566 4 1.5455394565253913 2.617788601971429 5 0.7583449644894497 1.6055775833005197 6 0.4295082455060757 1.0912323701521232 7 0.2841848004478157 0.8423515327082116 8 0.1724424657046155 0.5841558030905895 9 0.13800127403073428 0.5259204878609506 >10 0.5816523749975162 4.323470102544457 >50 0.042650111534133145 1.2410493805514005 >100 0.027860301092007482 2.4853484894633247 >500 0.005328470760841537 1.550788036744118 >1k 0.0031970824565049224 3.099255306555962 >5k 6.089680869533186E-4 1.9437067715277645 >10k+ 0.0027403563912899334 29.311673149111904 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 114519 5.9335008945405825 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 61970 3.210812620042787 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 34429 1.7838481151436678 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 33968 1.7599626121932124 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 28803 1.4923517168806257 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 21715 1.12510563247102 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 16920 0.8766653143637883 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 16422 0.8508627536927972 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15368 0.7962525148429489 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 14097 0.7303989915240142 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 13879 0.7191038946841026 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 13723 0.7110211648353584 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 13703 0.7099849174188527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13013 0.6742343815494075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10907 0.5651175285913616 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 10860 0.5626823471625731 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 10301 0.53371923187124 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10089 0.52273500925628 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 9829 0.5092637928417065 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 7926 0.4106648511611929 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 6254 0.3240345671413198 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6142 0.3182315816088881 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4823 0.24989106449033985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4748 0.24600513667844362 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4481 0.23217123366809306 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 4250 0.2202025760074527 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4038 0.2092183533924927 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2604 0.1349194136290369 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 2331 0.12077463639373465 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2197 0.11383177870314672 No Hit AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA 2185 0.11321003025324332 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.181237082528299E-5 0.0 3 0.0 0.0 0.0 5.181237082528299E-5 0.0 4 0.0 0.0 0.0 5.181237082528299E-5 0.0 5 0.0 0.0 0.0 5.181237082528299E-5 0.0 6 0.0 0.0 0.0 5.181237082528299E-5 0.0 7 0.0 0.0 0.0 5.181237082528299E-5 0.0 8 0.0 0.0 0.0 5.181237082528299E-5 0.0 9 0.0 0.0 0.0 1.0362474165056598E-4 0.0 10-11 0.0 0.0 0.0 4.663113374275469E-4 0.0 12-13 0.0 0.0 0.0 0.0013471216414573577 0.0 14-15 0.0 0.0 0.0 0.002901492766215847 0.0 16-17 2.5906185412641494E-5 0.0 0.0 0.005518017492892638 0.0 18-19 5.181237082528299E-5 0.0 0.0 0.008264073146632637 0.0 20-21 5.181237082528299E-5 0.0 0.0 0.011839126733577163 0.0 22-23 5.181237082528299E-5 0.0 0.0 0.018471110199213384 0.0 24-25 5.181237082528299E-5 0.0 0.0 0.02448134521494621 0.0 26-27 5.181237082528299E-5 0.0 0.0 0.03103561012434451 0.0 28-29 5.181237082528299E-5 0.0 0.0 0.04051727398537129 0.0 30-31 5.181237082528299E-5 0.0 0.0 0.053340835764628836 0.0 32-33 7.771855623792449E-5 0.0 0.0 0.07367719131355241 0.0 34-35 1.0362474165056598E-4 0.0 0.0 0.10113774785095239 0.0 36-37 1.0362474165056598E-4 0.0 0.0 0.13836493628891822 0.0 38-39 1.0362474165056598E-4 0.0 0.0 0.19639479161323514 0.0 40-41 1.0362474165056598E-4 0.0 0.0 0.30009725182003905 0.0 42-43 1.0362474165056598E-4 0.0 0.0 0.37711634105182223 0.0 44-45 1.0362474165056598E-4 0.0 0.0 0.4447055787934039 0.0 46-47 1.2953092706320747E-4 0.0 0.0 0.5359471638167272 0.0 48-49 1.5543711247584897E-4 0.0 0.0 0.6141320313920793 0.0 50-51 1.5543711247584897E-4 0.0 0.0 0.7006327844848892 0.0 52-53 1.5543711247584897E-4 0.0 0.0 0.8237389775657615 0.0 54-55 1.5543711247584897E-4 0.0 0.0 0.9399800315122839 0.0 56-57 1.5543711247584897E-4 0.0 0.0 1.0615059472829853 0.0 58-59 1.5543711247584897E-4 0.0 0.0 1.215285063892425 0.0 60-61 1.5543711247584897E-4 0.0 0.0 1.3582613011847935 0.0 62-63 1.5543711247584897E-4 0.0 0.0 1.4930511838867673 0.0 64-65 1.5543711247584897E-4 0.0 0.0 1.6548094056033005 0.0 66-67 1.5543711247584897E-4 0.0 0.0 1.830375624144772 0.0 68-69 1.5543711247584897E-4 0.0 0.0 2.065292913466605 0.0 70-71 1.5543711247584897E-4 0.0 0.0 2.4569685307203315 0.0 72-73 1.5543711247584897E-4 0.0 0.0 2.73675533317686 0.0 74-75 1.5543711247584897E-4 0.0 0.0 2.980947036876419 0.0 76-77 1.5543711247584897E-4 0.0 0.0 3.26723629187152 0.0 78-79 1.5543711247584897E-4 0.0 0.0 3.5303654171077197 0.0 80-81 2.0724948330113195E-4 0.0 0.0 3.820540599914717 0.0 82-83 2.0724948330113195E-4 0.0 0.0 4.229495642838676 0.0 84-85 2.0724948330113195E-4 0.0 0.0 4.598969659193768 0.0 86-87 2.0724948330113195E-4 0.0 0.0 4.9523818405930236 0.0 88-89 2.0724948330113195E-4 0.0 0.0 5.382217268959572 0.0 90-91 3.1087422495169794E-4 0.0 0.0 5.769100241911959 0.0 92-93 3.1087422495169794E-4 0.0 0.0 6.154532468481239 0.0 94-95 3.1087422495169794E-4 0.0 0.0 6.616051161607448 0.0 96-97 3.1087422495169794E-4 0.0 0.0 7.063010578531751 0.0 98-99 3.626865957769809E-4 0.0 0.0 7.602610514491661 0.0 100-101 3.626865957769809E-4 0.0 0.0 8.474353653627047 0.0 102-103 3.885927811896224E-4 0.0 0.0 9.136412128032513 0.0 104-105 4.6631133742754686E-4 0.0 0.0 9.71295428439085 0.0 106-107 5.181237082528299E-4 0.0 0.0 10.3264127549622 0.0 108-109 5.440298936654714E-4 0.0 0.0 10.848189235358213 0.0 110-111 5.699360790781128E-4 0.0 0.0 11.407348341304665 0.0 112-113 5.699360790781128E-4 0.0 0.0 12.17854957485359 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCA 775 0.0 59.213047 1 AAAAAGT 2025 0.0 50.621128 1 AAAAGTA 2060 0.0 50.25751 2 AGTACTC 2305 0.0 49.044506 5 AAAGTAC 2195 0.0 47.16528 3 TTATCAA 1005 0.0 45.587273 2 AAGTACT 2440 0.0 43.160965 4 GTGTAAG 165 3.3612378E-7 28.895782 1 TATCACG 110 7.7529496E-4 27.045576 2 GCCTAAC 90 0.009454728 26.4878 1 CGTACCT 45 0.009520049 26.451078 104-105 TAGTACA 310 1.2732926E-11 24.95108 4 GTGTAAC 195 1.444907E-6 24.45028 1 TAGATAG 270 1.713488E-9 24.240223 5 GTGTTAT 370 0.0 24.16117 1 CCCTTAC 325 2.3646862E-11 23.839022 1 ATAGTAC 355 3.6379788E-12 23.464289 3 ATTTACT 1015 0.0 23.448383 2 GATTACA 545 0.0 22.964195 1 TCGCGCT 260 0.0 22.882347 60-61 >>END_MODULE