FastQCFastQC Report
Thu 26 May 2016
SRR1364138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555174
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA586003.7680671101754526No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT403212.592700238044103No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG270861.7416700639285378No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC152930.9833626333773585No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG145650.9365511511895133No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC137950.8870390065677538No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC121180.7792054136707532No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG109920.7068019398472454No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT91180.586300954105457No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG84460.5430903551628307No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC64620.41551620590364813No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT61650.3964186644066838No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG59910.3852302057518966No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57790.37159829060928234No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG56880.36574685533580165No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA54800.3523721461392744No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG52320.3364253774818766No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC51230.3294165154510042No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC40690.26164274865706344No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40350.25945649811532345No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG39830.25611282081619163No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA36750.23630796296748788No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT34900.2244121879609613No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC29400.1890463703739903No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG22700.1459643744044075No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC18180.11690010249656951No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC14650.041.455615
AAGTACT18350.033.094574
AAAGTAC19200.030.0789833
CATGGGG68400.024.8944534
AAAAAGT25100.022.2934131
TATAGGG2755.4889824E-821.6473032
AAAAGTA27150.021.049312
ATGGGGA36900.020.9767275
ATAACGC1754.7640066E-420.4129543
GTACAAA13000.020.1479911
ATGGGGG26600.020.1456645
CATGGGT29300.020.1168614
GTGTAGC5450.019.6606751
ATGGGTA18700.019.4226725
ATGGGAG23450.018.0275295
TAACGCA3003.0117753E-617.8613344
TATAACG2000.001170606617.8590242
ATGGGAT22850.017.7191725
GTGTAAG5052.5465852E-1117.6816311
CGGGAGT4752.0008883E-1017.5479774
Produced by FastQC (version 0.11.5)