##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364138_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1555174 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08113883076749 33.0 31.0 34.0 30.0 34.0 2 31.913241862325375 33.0 31.0 34.0 30.0 34.0 3 32.14250173935521 34.0 31.0 34.0 30.0 34.0 4 35.61082489804999 37.0 35.0 37.0 33.0 37.0 5 35.744018354216315 37.0 35.0 37.0 33.0 37.0 6 35.86853561080625 37.0 35.0 37.0 35.0 37.0 7 35.84663388148207 37.0 35.0 37.0 35.0 37.0 8 35.89235481045851 37.0 35.0 37.0 35.0 37.0 9 37.558513066705075 39.0 37.0 39.0 35.0 39.0 10-11 37.519962074983255 39.0 37.0 39.0 34.5 39.0 12-13 37.4993187257503 39.0 37.0 39.0 34.5 39.0 14-15 38.80184564556764 40.0 38.0 41.0 34.0 41.0 16-17 38.78779030513628 40.0 38.0 41.0 34.0 41.0 18-19 38.86840347125145 40.0 38.0 41.0 34.5 41.0 20-21 38.91563419913142 40.0 38.5 41.0 35.0 41.0 22-23 38.874874772855 40.0 38.0 41.0 35.0 41.0 24-25 38.84709942424449 40.0 38.0 41.0 35.0 41.0 26-27 38.679287333764584 40.0 38.0 41.0 34.0 41.0 28-29 38.53775815439302 40.0 38.0 41.0 34.0 41.0 30-31 38.40661366509471 40.0 38.0 41.0 34.0 41.0 32-33 38.12289010747351 40.0 37.0 41.0 34.0 41.0 34-35 37.91535095108328 40.0 37.0 41.0 33.0 41.0 36-37 37.79723490747659 40.0 37.0 41.0 33.0 41.0 38-39 37.700730914997294 40.0 37.0 41.0 33.0 41.0 40-41 37.49387592642367 40.0 37.0 41.0 33.0 41.0 42-43 37.3387800979183 40.0 37.0 41.0 32.5 41.0 44-45 37.102826436141555 40.0 36.5 41.0 31.5 41.0 46-47 36.78294872470862 40.0 36.0 41.0 31.0 41.0 48-49 36.45197964986555 40.0 36.0 41.0 30.0 41.0 50-51 36.28521760266054 40.0 36.0 41.0 30.0 41.0 52-53 36.16575315688148 40.0 35.5 41.0 29.5 41.0 54-55 35.98638673228848 39.0 35.0 41.0 28.5 41.0 56-57 35.73566559111714 39.0 35.0 41.0 28.0 41.0 58-59 35.57642199522368 39.0 35.0 41.0 28.0 41.0 60-61 35.41884766592034 39.0 35.0 41.0 28.0 41.0 62-63 35.09099014001006 38.0 35.0 40.0 28.0 41.0 64-65 34.81376874870593 38.0 35.0 40.0 27.0 41.0 66-67 34.39448704775157 37.0 34.0 40.0 26.0 41.0 68-69 34.07626156301481 37.0 34.0 39.5 26.0 41.0 70-71 33.651280499802596 36.0 34.0 39.0 26.0 41.0 72-73 33.35082119428437 35.5 34.0 39.0 26.0 40.0 74-75 33.02199528798707 35.0 34.0 37.5 26.0 39.5 76-77 32.57764983210882 35.0 33.0 37.0 25.5 39.0 78-79 32.20056662469923 35.0 33.0 36.5 24.5 39.0 80-81 31.952135259462928 35.0 33.0 36.0 24.0 37.5 82-83 31.726285611770773 35.0 33.0 36.0 24.0 37.0 84-85 31.49080392290509 35.0 33.0 35.0 23.0 37.0 86-87 31.228899788705316 35.0 33.0 35.0 20.5 36.0 88-89 31.03781859779034 35.0 32.0 35.0 20.0 36.0 90-91 30.89054890320954 35.0 32.0 35.0 19.5 36.0 92-93 30.703307797069655 35.0 32.0 35.0 18.5 36.0 94-95 30.628032940365514 35.0 32.0 35.0 18.0 35.0 96-97 30.53883231072536 35.0 32.0 35.0 17.5 35.0 98-99 30.441373762678644 35.0 32.0 35.0 14.0 35.0 100-101 30.243522911262662 35.0 32.0 35.0 8.5 35.0 102-103 28.962496479493613 33.5 29.5 34.5 7.0 35.0 104-105 29.83595758416743 34.0 31.0 35.0 6.0 35.0 106-107 29.913200066359135 35.0 31.0 35.0 2.0 35.0 108-109 29.84624421447375 35.0 31.0 35.0 2.0 35.0 110-111 29.83594665291472 35.0 31.0 35.0 2.0 35.0 112-113 29.77014115462321 35.0 31.0 35.0 2.0 35.0 114-115 29.690244307067893 35.0 31.0 35.0 2.0 35.0 116-117 29.495763818067946 35.0 31.0 35.0 2.0 35.0 118-119 29.34763216206032 35.0 31.0 35.0 2.0 35.0 120-121 29.19616840302114 35.0 31.0 35.0 2.0 35.0 122-123 28.907216491530853 35.0 31.0 35.0 2.0 35.0 124-125 27.934766784938535 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.295590326963044 1101 2 0.5990225854675835 1101 3 0.4906515299834382 1101 4 0.5389448872168288 1101 5 0.2739540195502741 1101 6 0.30544117575461627 1101 7 0.3087910998490955 1101 8 0.3216799230948979 1101 9 0.32324214228346904 1101 10-11 0.2981529202715478 1101 12-13 0.3168412892289325 1101 14-15 0.42414646251728527 1101 16-17 0.5348286521570742 1101 18-19 0.39237845236329605 1101 20-21 0.30428940286520856 1101 22-23 0.2927840579862391 1101 24-25 0.34264907285967894 1101 26-27 0.34314144544775615 1101 28-29 0.3831824088299527 1101 30-31 0.28687801093421683 1101 32-33 0.23452505025757375 1101 34-35 0.3370058806242966 1101 36-37 0.259470889877484 1101 38-39 0.232005499042792 1101 40-41 0.1977532515862208 1101 42-43 0.12337030443558916 1101 44-45 0.1879898065141532 1101 46-47 0.18935587498063455 1101 48-49 0.19951802560898102 1101 50-51 0.22408846724924558 1101 52-53 0.15371266776252668 1101 54-55 0.2045266561842496 1101 56-57 0.21214107788865988 1101 58-59 0.24436856916207716 1101 60-61 0.2089600082813945 1101 62-63 0.23907743162867945 1101 64-65 0.09678201395048092 1101 66-67 0.004764918080063296 1101 68-69 0.17747438983678876 1101 70-71 0.24914269429744706 1101 72-73 0.12205230602756245 1101 74-75 0.11039865464603338 1101 76-77 0.03010742653835763 1101 78-79 0.08431324661790995 1101 80-81 0.10059592521349359 1101 82-83 0.1137365280022884 1101 84-85 0.055364867782852656 1101 86-87 0.0896896793693358 1101 88-89 0.09917283455118664 1101 90-91 0.14477015859678488 1101 92-93 0.21741502199715868 1101 94-95 0.10031652760897813 1101 96-97 0.1447594944519004 1101 98-99 0.0837187191621176 1101 100-101 0.12523192991871923 1101 102-103 0.1320260163484548 1101 104-105 0.22111247586409988 1101 106-107 0.23632987656923277 1101 108-109 0.2732456084885193 1101 110-111 0.21997346619566116 1101 112-113 0.1993488863413262 1101 114-115 0.19215748694711365 1101 116-117 0.2429895929248218 1101 118-119 0.30777593289566596 1101 120-121 0.42402454580834004 1101 122-123 0.5011433024420064 1101 124-125 0.43843921387890816 1102 1 0.306778958914947 1102 2 0.6001906750248871 1102 3 0.4238977937176287 1102 4 0.4494356431392603 1102 5 0.2970819289375797 1102 6 0.221636875044112 1102 7 0.17279177831350268 1102 8 0.1575965096650691 1102 9 0.2838333486308855 1102 10-11 0.25591116078718557 1102 12-13 0.2698175806670946 1102 14-15 0.35793926702530143 1102 16-17 0.41128188522844766 1102 18-19 0.2882227636276795 1102 20-21 0.18327651204144502 1102 22-23 0.20572086656504496 1102 24-25 0.2361202663916515 1102 26-27 0.2762996972711278 1102 28-29 0.3273257532825653 1102 30-31 0.23598752639755105 1102 32-33 0.2910792049097637 1102 34-35 0.284289478747219 1102 36-37 0.2683644275039896 1102 38-39 0.1498768169599103 1102 40-41 0.18967961935162236 1102 42-43 0.1711423027017318 1102 44-45 0.27283752894683033 1102 46-47 0.38019443814378917 1102 48-49 0.31370179523261754 1102 50-51 0.36634351332828174 1102 52-53 0.3256376220604196 1102 54-55 0.22366717370181988 1102 56-57 0.3179387447249411 1102 58-59 0.35191139722788733 1102 60-61 0.3850594033172996 1102 62-63 0.31361108157874185 1102 64-65 0.25963420294051076 1102 66-67 0.2643265546869955 1102 68-69 0.2689741731051072 1102 70-71 0.21696058314236666 1102 72-73 0.3052894293384725 1102 74-75 0.24519765956489437 1102 76-77 0.29179105481524203 1102 78-79 0.2550391093042492 1102 80-81 0.3751686810982342 1102 82-83 0.3613479563583333 1102 84-85 0.32884670943679595 1102 86-87 0.4218227903171119 1102 88-89 0.3903395106409704 1102 90-91 0.42974801427232734 1102 92-93 0.4196110793650938 1102 94-95 0.39588210307872274 1102 96-97 0.364327538778241 1102 98-99 0.41150287324892076 1102 100-101 0.33707537425637213 1102 102-103 0.3145515058793684 1102 104-105 0.3963413633709294 1102 106-107 0.3344840764523873 1102 108-109 0.3446105469555647 1102 110-111 0.44067671340170733 1102 112-113 0.38698000901145946 1102 114-115 0.341301491017898 1102 116-117 0.33386490624345555 1102 118-119 0.4014018540053321 1102 120-121 0.4612372820929913 1102 122-123 0.4374994774104408 1102 124-125 0.33648109020992933 1103 1 0.287862443931175 1103 2 0.6485406778396232 1103 3 0.46761286755749154 1103 4 0.5451544324282551 1103 5 0.20736903182550037 1103 6 0.2485633729518284 1103 7 0.24503774855237737 1103 8 0.24429651529453622 1103 9 0.36291180465535433 1103 10-11 0.3413775622433377 1103 12-13 0.294031331581877 1103 14-15 0.4084097029338025 1103 16-17 0.4472816788146048 1103 18-19 0.2832829989394625 1103 20-21 0.21370407016570425 1103 22-23 0.2896367247774947 1103 24-25 0.28951155197458434 1103 26-27 0.24306500473392845 1103 28-29 0.2665107939085658 1103 30-31 0.35590083258433935 1103 32-33 0.3306933986760612 1103 34-35 0.4151227338934902 1103 36-37 0.43463265290790787 1103 38-39 0.41028157573718005 1103 40-41 0.41094108939346796 1103 42-43 0.32500074033660553 1103 44-45 0.3944761733708404 1103 46-47 0.26946026164118564 1103 48-49 0.21867908971011474 1103 50-51 0.3077023731732922 1103 52-53 0.27608310065905783 1103 54-55 0.4170100458565926 1103 56-57 0.18936862969613344 1103 58-59 0.054746209860411454 1103 60-61 -0.027539534589664072 1103 62-63 0.12018761420151236 1103 64-65 0.016777060083370543 1103 66-67 0.06787499626249627 1103 68-69 0.10311746184054016 1103 70-71 0.017049997865299815 1103 72-73 0.01526681764043758 1103 74-75 0.07285767720389913 1103 76-77 0.09991916275091484 1103 78-79 0.04564286978913401 1103 80-81 0.019353346426331797 1103 82-83 0.06061593781861774 1103 84-85 -0.005371451228228352 1103 86-87 -0.02443952414499151 1103 88-89 -0.014418234569291144 1103 90-91 0.022352581647680836 1103 92-93 0.07656112721557662 1103 94-95 0.13243883873765228 1103 96-97 0.08944997971573443 1103 98-99 0.08152773673431213 1103 100-101 0.16260172955088592 1103 102-103 0.05772511868453023 1103 104-105 0.16727041330431192 1103 106-107 0.10176260378345603 1103 108-109 0.1974460163594074 1103 110-111 0.22326129616427792 1103 112-113 0.21053848980558953 1103 114-115 0.08087984383545077 1103 116-117 0.09738060307781993 1103 118-119 0.12309153894275227 1103 120-121 0.09167403501562532 1103 122-123 0.2357328564050185 1103 124-125 0.25273336545340186 1104 1 0.302650749981872 1104 2 0.5796171477953287 1104 3 0.03689378718846825 1104 4 0.3981743284041315 1104 5 0.15875502583622847 1104 6 0.1878610922870294 1104 7 0.21478465559499682 1104 8 0.1968931412127759 1104 9 0.2978087158960534 1104 10-11 0.29265400097119 1104 12-13 0.21406932424922331 1104 14-15 0.36693244108673895 1104 16-17 0.41969151573742636 1104 18-19 0.2928629179532507 1104 20-21 0.250394778846605 1104 22-23 0.18991214123487055 1104 24-25 0.17910698548944026 1104 26-27 0.1947219409274581 1104 28-29 0.30019858307927194 1104 30-31 0.33863814221592037 1104 32-33 0.32726892148491515 1104 34-35 0.21146747251483333 1104 36-37 0.004391657063273158 1104 38-39 0.05009124046487301 1104 40-41 0.06974045923884375 1104 42-43 -0.06576896009690358 1104 44-45 -0.052992712928819685 1104 46-47 -0.05877684839724395 1104 48-49 0.0011672945648655286 1104 50-51 -0.005214581344510805 1104 52-53 -0.0740307263592328 1104 54-55 -0.05360096697187089 1104 56-57 0.00666926854152905 1104 58-59 0.07844983022981467 1104 60-61 0.09443913324755471 1104 62-63 0.06828796242159996 1104 64-65 0.060018161984878304 1104 66-67 -0.012433721318352298 1104 68-69 0.05511751781499896 1104 70-71 -0.017818686778994675 1104 72-73 -0.029701667263402953 1104 74-75 0.06966271372724009 1104 76-77 0.027287048286090965 1104 78-79 -0.0012103342333631417 1104 80-81 0.0456750618668913 1104 82-83 0.01764117493675954 1104 84-85 4.645310720547968E-4 1104 86-87 0.04565459028743746 1104 88-89 0.045309832647205894 1104 90-91 0.15207662385832066 1104 92-93 0.12969046799842232 1104 94-95 0.058286391548822536 1104 96-97 0.14733503247166624 1104 98-99 0.13400324422384458 1104 100-101 0.16470187670480385 1104 102-103 0.13724181601440222 1104 104-105 0.22803115414107467 1104 106-107 0.19979755026155743 1104 108-109 0.23313369579070553 1104 110-111 0.25697698050365503 1104 112-113 0.32828546976231365 1104 114-115 0.3809279192713504 1104 116-117 0.2145590003226978 1104 118-119 0.22263052294731267 1104 120-121 0.29976785042657283 1104 122-123 0.3151419317805271 1104 124-125 0.3711023248144656 1105 1 0.16262872700574604 1105 2 0.4626507979556145 1105 3 0.03342605647263497 1105 4 0.3057103895474924 1105 5 0.07578738540275509 1105 6 0.06961228889832682 1105 7 0.0794639756060107 1105 8 0.03643696367917215 1105 9 0.08989805695321706 1105 10-11 0.026398735733444312 1105 12-13 0.07782787517291467 1105 14-15 0.1432471594797704 1105 16-17 0.11324783417071416 1105 18-19 -0.026191618513422554 1105 20-21 -0.04296184536268299 1105 22-23 -0.03172624682535741 1105 24-25 -0.059687456373154646 1105 26-27 -0.02783092605554316 1105 28-29 -0.016456440616153145 1105 30-31 0.04976067018125718 1105 32-33 -0.004040520570086414 1105 34-35 -0.05534003216948946 1105 36-37 -0.03668478249638696 1105 38-39 -0.054228918550485616 1105 40-41 -0.11286930783266058 1105 42-43 -0.11189325670850536 1105 44-45 0.0020865579448994254 1105 46-47 0.00938614974678842 1105 48-49 0.010298763444097858 1105 50-51 0.028740096909871227 1105 52-53 0.047184814639315675 1105 54-55 0.01316394959834355 1105 56-57 0.07596431129985604 1105 58-59 0.009908682193383811 1105 60-61 0.032527633642722265 1105 62-63 -0.006218851676656811 1105 64-65 -0.13112902342549404 1105 66-67 -0.02757359237737944 1105 68-69 0.02104702653095103 1105 70-71 -0.04794061976875952 1105 72-73 0.006914490615294255 1105 74-75 -0.08814937463699124 1105 76-77 -0.09646472406027584 1105 78-79 -0.05020109583168875 1105 80-81 -0.05483603250333857 1105 82-83 0.004178002740172104 1105 84-85 -0.02938248472587901 1105 86-87 0.025007865169104804 1105 88-89 0.014900957151020577 1105 90-91 0.013618446792406758 1105 92-93 -0.02675850470670227 1105 94-95 -0.0042987106774532435 1105 96-97 6.637505509310415E-4 1105 98-99 -0.02987293277694647 1105 100-101 -0.08556971039991623 1105 102-103 -0.2313237156599044 1105 104-105 -0.07330417379110443 1105 106-107 -0.05912578817297742 1105 108-109 0.032206044523348965 1105 110-111 -0.040179088099101534 1105 112-113 0.015917563217875852 1105 114-115 -0.11446305515654132 1105 116-117 -0.060658454365917436 1105 118-119 -0.18823821537888108 1105 120-121 -0.13510138075251632 1105 122-123 0.014996554977468435 1105 124-125 0.08758407297700188 1106 1 0.2737390154720316 1106 2 0.5069174335968221 1106 3 0.415195071907803 1106 4 0.4911111468508196 1106 5 0.28645626615921316 1106 6 0.2170981687874871 1106 7 0.1973022060264782 1106 8 0.11023701867885194 1106 9 0.23624762929542698 1106 10-11 0.2797658797691511 1106 12-13 0.29539198858508087 1106 14-15 0.353142519057819 1106 16-17 0.3400323270806922 1106 18-19 0.09605945503808044 1106 20-21 0.12623692208032367 1106 22-23 0.28244939254595636 1106 24-25 0.2654415813439286 1106 26-27 0.26965777433015603 1106 28-29 0.33837736162473675 1106 30-31 0.3440999336085824 1106 32-33 0.3108370698797174 1106 34-35 0.2324124904121092 1106 36-37 0.2529879693915902 1106 38-39 0.18816596506877659 1106 40-41 0.23646470241984474 1106 42-43 0.31065449782368404 1106 44-45 0.3089297876955044 1106 46-47 0.1820625894339969 1106 48-49 0.09482763475188705 1106 50-51 0.11755242106954 1106 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20-21 -0.8092421908101599 2216 22-23 -0.7143868117772172 2216 24-25 -0.6576514492624028 2216 26-27 -0.6339147203580708 2216 28-29 -0.8503867079814 2216 30-31 -0.6417365165485762 2216 32-33 -0.8649100898597055 2216 34-35 -0.7585315999143134 2216 36-37 -0.7295946922881669 2216 38-39 -0.6955887979648097 2216 40-41 -0.7874022662714424 2216 42-43 -0.7667050695084896 2216 44-45 -0.7130714765935977 2216 46-47 -0.7164044600313133 2216 48-49 -0.7333616223026667 2216 50-51 -0.7690922083357137 2216 52-53 -0.8870825081552525 2216 54-55 -1.0206313519813435 2216 56-57 -1.0909090344092505 2216 58-59 -1.1670860047190814 2216 60-61 -1.1789847948859773 2216 62-63 -1.0715161604158112 2216 64-65 -1.185758165670002 2216 66-67 -1.1675903702198838 2216 68-69 -1.2225161076724333 2216 70-71 -1.3507811453454508 2216 72-73 -1.072089197762665 2216 74-75 -1.0373058618925128 2216 76-77 -1.1004528178488542 2216 78-79 -0.9784928342272252 2216 80-81 -0.9517113076331079 2216 82-83 -1.0403805314495962 2216 84-85 -1.0795152650835007 2216 86-87 -1.092959661869319 2216 88-89 -1.1838087661925343 2216 90-91 -1.2230181296535498 2216 92-93 -1.2173524852888633 2216 94-95 -1.335849671422796 2216 96-97 -1.3931692527827835 2216 98-99 -1.402657611303617 2216 100-101 -1.5611818955968246 2216 102-103 -1.5351478399120992 2216 104-105 -1.4199681492593648 2216 106-107 -1.545499020552029 2216 108-109 -1.4771728755880211 2216 110-111 -1.6119368340362357 2216 112-113 -1.6247946490159002 2216 114-115 -1.7529314573027541 2216 116-117 -1.7546565511703847 2216 118-119 -1.7777666125173468 2216 120-121 -1.7663260258858493 2216 122-123 -1.929518239433083 2216 124-125 -2.3357181773269318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52.0 3 23.0 4 37.0 5 191.0 6 557.0 7 1157.0 8 1773.0 9 3539.0 10 7477.0 11 9813.0 12 8397.0 13 10052.0 14 14800.0 15 15123.0 16 8388.0 17 7368.0 18 7446.0 19 7359.0 20 7315.0 21 7005.0 22 7052.0 23 7821.0 24 8904.0 25 10470.0 26 12450.0 27 15305.0 28 19154.0 29 24531.0 30 30741.0 31 39099.0 32 50747.0 33 68071.0 34 95849.0 35 148940.0 36 251827.0 37 433620.0 38 211175.0 39 1546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.76384918506173 18.21721426285261 11.811823286324145 23.207113265761517 2 13.545730399718616 22.59188551212119 37.86990352816961 25.99248055999059 3 21.547075008664667 28.445691041540876 28.885643792595907 21.121590157198554 4 13.277742554852384 18.294351628820955 34.78022394921726 33.647681867109405 5 11.912429091535738 38.978918114628975 34.53890047030107 14.569752323534216 6 31.089327672840305 31.511485390706483 19.762189844293573 17.636997092159636 7 24.30493308144298 32.796394487047756 23.664811783118804 19.23386064839047 8 27.403943224359463 32.67737243549597 22.730382580984507 17.18830175916007 9 28.758068229021315 14.8801999004613 21.33291837440698 35.02881349611041 10-11 23.870737293704757 27.44721812478861 26.880593425558814 21.801451155947824 12-13 26.79881524770544 25.069208079516788 27.043334385789148 21.088642286988623 14-15 26.47509414707875 23.705998116576154 26.384491750455663 23.43441598588943 16-17 22.17665719607154 27.218889062861194 30.902197163065388 19.70225657800188 18-19 23.569195472660937 25.24267381013314 32.14807474919205 19.04005596801387 20-21 21.36860789951462 25.576214584693712 32.637525829144884 20.417651686646778 22-23 20.4128605545103 26.737908426967017 28.837094755956567 24.012136262566116 24-25 21.20653380264845 27.855307508998994 27.869196630087696 23.068962058264862 26-27 22.76465527330061 24.48652047938044 28.939687777702044 23.809136469616902 28-29 22.219557276838778 24.196832437343687 31.346530814466966 22.23707947135057 30-31 24.044061950624172 25.54498724901522 30.25773964842519 20.15321115193541 32-33 19.935293414113147 25.803414923346196 31.74371485119993 22.517576811340724 34-35 18.752093818501223 26.9055597030041 32.25239249909817 22.089953979396505 36-37 23.181489659677954 25.467246751810407 29.74843972442955 21.60282386408209 38-39 24.497548184318926 24.324191376656245 29.53052198660729 21.647738452417542 40-41 21.732951896670016 26.43281487011413 29.58436135401013 22.24987187920572 42-43 24.00599547060329 25.524217868868693 28.970263134543146 21.499523525984873 44-45 25.32642649632774 23.91086142129434 28.819636902365907 21.943075180012013 46-47 22.330073676643256 25.44699178355605 32.395796226017154 19.82713831378354 48-49 24.101515328831372 25.22135786735118 30.875258974236967 19.801867829580484 50-51 21.2127388960978 26.258830201636602 30.98097061807875 21.54746028418685 52-53 20.56509432385058 27.482583942375584 27.248076421030703 24.70424531274314 54-55 21.483480305097693 28.148586589024767 26.328725917485762 24.03920718839178 56-57 23.023629510267018 25.208143911870955 27.178952323019807 24.589274254842223 58-59 22.76076503336604 24.677528045093347 29.42143451472311 23.1402724068175 60-61 24.30133219819776 26.101741670063927 28.461959883588587 21.134966248149727 62-63 20.325667738786784 26.356825667095773 29.984168974018345 23.3333376200991 64-65 18.94533987836731 27.140017772930875 30.778035126616054 23.136607222085757 66-67 23.118216996940536 26.04428829185673 28.330173986962233 22.507320724240504 68-69 24.288825559069274 24.91701893164366 28.199545517093267 22.5946099921938 70-71 21.697250597039304 26.75440175825985 28.26265742611438 23.285690218586474 72-73 23.840354841323222 26.055862559430647 27.688284397758707 22.41549820148742 74-75 25.10606530201765 24.353641457483214 27.67320569916935 22.86708754132978 76-77 22.18494522156363 25.786342878674667 31.33565761773281 20.693054282028893 78-79 23.625459273367483 25.59713575458438 30.128107851597314 20.64929712045083 80-81 20.877438794630056 26.51102063177497 30.25728953801954 22.35425103557544 82-83 20.32512117615135 27.505539573063846 26.80944383072248 25.359895420062323 84-85 21.216854191235193 28.00005015515949 25.985355979459534 24.797739674145785 86-87 22.614864960448156 25.086774856061123 26.94962107133993 25.348739112150792 88-89 22.317309831568686 24.664667747788993 28.91133082214595 24.106691598496376 90-91 23.663622205618147 26.199383477347226 27.89520658138575 22.24178773564887 92-93 20.170154593633896 26.307120618014444 29.10593927110407 24.41678551724759 94-95 18.880931145947137 27.1006372981798 29.91301854022643 24.105413015646633 96-97 22.785676186017827 25.97900947862575 27.727769470150577 23.507544865205844 98-99 23.77121232581618 24.9985531570114 27.5137771604581 23.716457356714315 100-101 21.376450480782214 26.549955181863893 27.710211204662627 24.36338313269126 102-103 23.15664757911794 25.752468466962252 27.418743951411955 23.672140002507852 104-105 24.3422267014572 24.14493812502854 27.53039080181808 23.98244437169618 106-107 21.7657657194142 25.560831705951603 30.741227493334783 21.932175081299416 108-109 23.034693354505585 25.62038324215181 29.274171946062218 22.070751457280387 110-111 20.31321848505487 26.491442330510683 29.7183908378625 23.476948346571948 112-113 19.85016823756997 27.178599357918998 26.59500920933487 26.376223195176163 114-115 20.51211620232482 27.862022604903093 25.815809279975298 25.810051912796794 116-117 21.840974792640424 25.25283909257108 26.491721698811098 26.414464415977395 118-119 21.409561468050402 24.930152866176496 28.26220202415193 25.39808364162117 120-121 22.588128993257083 26.207535217834483 27.410424028836662 23.79391176007177 122-123 19.608224545221592 26.566730466447886 28.232415969000574 25.592629019329948 124-125 18.537192476721646 27.36449588511436 28.859531883330607 25.238779754833384 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 808.0 1 679.5 2 1839.5 3 2505.5 4 1265.5 5 694.5 6 1066.5 7 2273.5 8 3426.0 9 3602.0 10 2917.5 11 2120.0 12 1905.0 13 1998.0 14 2301.0 15 2731.0 16 2989.5 17 3197.5 18 3479.5 19 3664.5 20 3869.5 21 4345.5 22 4917.5 23 5531.0 24 6263.0 25 7085.5 26 8453.0 27 10066.0 28 11243.5 29 12487.0 30 14585.0 31 16893.0 32 18921.0 33 21055.0 34 23435.0 35 25605.5 36 28059.5 37 30194.0 38 31056.5 39 31518.0 40 31774.0 41 33450.5 42 35363.5 43 36933.0 44 38978.5 45 40952.5 46 43598.0 47 50103.0 48 58910.5 49 86798.5 50 189357.0 51 189542.0 52 78264.5 53 40852.0 54 34285.5 55 30364.5 56 26640.5 57 23696.0 58 21838.5 59 19777.5 60 17327.5 61 14110.0 62 11389.5 63 9314.5 64 7323.5 65 5709.0 66 4087.5 67 3222.5 68 2532.5 69 1694.5 70 1361.0 71 1169.0 72 1005.5 73 890.0 74 685.5 75 533.5 76 330.5 77 148.5 78 89.0 79 62.5 80 38.0 81 12.5 82 5.5 83 1.0 84 2.0 85 2.0 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15155860373180105 2 3.21507432608827E-4 3 4.501104056523578E-4 4 0.0 5 0.0 6 0.0043725010834800475 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005208420408262998 14-15 0.0017682908793485487 16-17 6.108641219567713E-4 18-19 0.0 20-21 1.607537163044135E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.250552028261789E-4 30-31 0.0 32-33 0.0 34-35 6.43014865217654E-5 36-37 0.0 38-39 0.0 40-41 6.43014865217654E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.645222978264811E-4 96-97 0.0027971146636967953 98-99 0.004115295137392986 100-101 0.0 102-103 0.0037937877047841593 104-105 0.022184012850009066 106-107 0.017586456563702838 108-109 0.01983700859196463 110-111 0.01626827609000665 112-113 0.014789341900006045 114-115 0.041410157320016926 116-117 0.04038133353566868 118-119 0.022505520282617895 120-121 0.013439010683048971 122-123 0.0031186220963056225 124-125 0.0017682908793485487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1555174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.54527115134189 #Duplication Level Percentage of deduplicated Percentage of total 1 87.63964742744447 49.55607627392851 2 8.729324115352046 9.872039981410582 3 1.81937242603475 3.08630721466229 4 0.6427439623457548 1.4537652652691433 5 0.30292920104773113 0.8564606906451661 6 0.18413291098015766 0.6247107227555347 7 0.11833252536783997 0.46838013130632883 8 0.08471550415716925 0.3832208922631813 9 0.058472997175046955 0.2975734332265211 >10 0.3620769856387357 3.818030113351961 >50 0.0306370813463728 1.205912957513669 >100 0.020506088083299324 2.497069565564256 >500 0.002050608180780221 0.7308080381814313 >1k 0.0025974370289882794 4.1506247939616845 >5k 0.0012303649084681324 4.66104635902536 >10k+ 0.0012303649084681324 16.33797356693444 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 54799 3.5236571599062225 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 43358 2.787983852610705 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 28434 1.8283484677598778 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17393 1.1183957550730659 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 15096 0.9706952405325706 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14972 0.9627218562038717 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14372 0.9241409642908125 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12806 0.8234448363977279 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10124 0.650988249546353 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 9360 0.6018619138437242 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7184 0.4619418791723627 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7166 0.46078445241497096 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6863 0.441301101998876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6306 0.40548517400625267 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6266 0.4029131145453821 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6192 0.3981548045427714 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5750 0.36973354750015114 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5210 0.3350107447783978 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4871 0.3132125408475193 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4679 0.30086665543534036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4646 0.2987447063801221 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4457 0.28659172542750844 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4430 0.28485558529142074 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 4278 0.2750817593401124 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 3899 0.25071149594836334 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3148 0.20242107957051753 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2684 0.17258518982441837 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 2529 0.16261845941354472 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2527 0.16248985644050118 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2060 0.13246106223483675 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.12648102398831257 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1806 0.11612848465830831 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.2860297304353083E-4 0.0 0.0 0.0 0.0 6 1.2860297304353083E-4 0.0 0.0 0.0 0.0 7 1.2860297304353083E-4 0.0 0.0 0.0 0.0 8 1.2860297304353083E-4 0.0 0.0 0.0 0.0 9 1.2860297304353083E-4 0.0 0.0 0.0 0.0 10-11 1.2860297304353083E-4 0.0 0.0 6.430148652176541E-5 0.0 12-13 1.2860297304353083E-4 0.0 0.0 3.215074326088271E-4 0.0 14-15 1.2860297304353083E-4 0.0 0.0 0.001286029730435308 0.0 16-17 1.2860297304353083E-4 0.0 0.0 0.002411305744566203 0.0 18-19 1.6075371630441354E-4 0.0 0.0 0.003279375812610036 0.0 20-21 1.9290445956529623E-4 0.0 0.0 0.004822611489132406 0.0 22-23 1.9290445956529623E-4 0.0 0.0 0.007555424666307435 0.0 24-25 1.9290445956529623E-4 0.0 0.0 0.010995554195221885 0.0 26-27 1.9290445956529623E-4 0.0 0.0 0.013471161426309854 0.0 28-29 1.9290445956529623E-4 0.0 0.0 0.01816516994239873 0.0 30-31 1.9290445956529623E-4 0.0 0.0 0.02626715724414117 0.0 32-33 1.9290445956529623E-4 0.0 0.0 0.04105649914414721 0.0 34-35 1.9290445956529623E-4 0.0 0.0 0.06333696422393893 0.0 36-37 1.9290445956529623E-4 0.0 0.0 0.09420167775438633 0.0 38-39 1.9290445956529623E-4 0.0 0.0 0.14303865676766714 0.0 40-41 1.9290445956529623E-4 0.0 0.0 0.22071485248595976 0.0 42-43 2.2505520282617894E-4 0.0 0.0 0.2854986001566384 0.0 44-45 2.5720594608706165E-4 0.0 0.0 0.3507646089762303 0.0 46-47 2.5720594608706165E-4 0.0 0.0 0.4346458981438733 0.0 48-49 2.5720594608706165E-4 0.0 0.0 0.5171125546080374 0.0 50-51 2.5720594608706165E-4 0.0 0.0 0.6017333108706807 0.0 52-53 2.5720594608706165E-4 0.0 0.0 0.7007897508574603 0.0 54-55 2.5720594608706165E-4 0.0 0.0 0.8119348703103318 0.0 56-57 2.5720594608706165E-4 0.0 0.0 0.9416952701112544 0.0 58-59 2.5720594608706165E-4 0.0 0.0 1.0919678441126202 0.0 60-61 2.5720594608706165E-4 6.430148652176541E-5 0.0 1.2347171441909395 0.0 62-63 2.5720594608706165E-4 6.430148652176541E-5 0.0 1.3739620132538226 0.0 64-65 2.5720594608706165E-4 6.430148652176541E-5 0.0 1.5393132858445422 0.0 66-67 2.5720594608706165E-4 6.430148652176541E-5 0.0 1.7196468047948332 0.0 68-69 3.21507432608827E-4 6.430148652176541E-5 0.0 1.947466971541448 0.0 70-71 3.21507432608827E-4 6.430148652176541E-5 0.0 2.2614511302272287 0.0 72-73 3.21507432608827E-4 6.430148652176541E-5 0.0 2.511841118742983 0.0 74-75 3.21507432608827E-4 6.430148652176541E-5 0.0 2.7573441942830836 0.0 76-77 3.21507432608827E-4 6.430148652176541E-5 0.0 3.0456720598466793 0.0 78-79 3.21507432608827E-4 6.430148652176541E-5 0.0 3.3441274095374536 0.0 80-81 3.8580891913059245E-4 6.430148652176541E-5 0.0 3.6520350777469273 0.0 82-83 3.8580891913059245E-4 6.430148652176541E-5 0.0 4.032153315320343 0.0 84-85 3.8580891913059245E-4 6.430148652176541E-5 0.0 4.402851385118321 0.0 86-87 3.8580891913059245E-4 6.430148652176541E-5 0.0 4.785927491071739 0.0 88-89 3.8580891913059245E-4 6.430148652176541E-5 0.0 5.231118833005182 0.0 90-91 4.501104056523579E-4 6.430148652176541E-5 0.0 5.657116181211878 0.0 92-93 4.501104056523579E-4 6.430148652176541E-5 0.0 6.08478536806814 0.0 94-95 4.501104056523579E-4 6.430148652176541E-5 0.0 6.558558720760507 0.0 96-97 4.501104056523579E-4 6.430148652176541E-5 0.0 7.044356451432444 0.0 98-99 4.501104056523579E-4 6.430148652176541E-5 0.0 7.595388040180713 0.0 100-101 4.501104056523579E-4 6.430148652176541E-5 0.0 8.343567986604715 0.0 102-103 4.501104056523579E-4 6.430148652176541E-5 0.0 8.983271325266497 0.0 104-105 5.144118921741233E-4 6.430148652176541E-5 0.0 9.588573368639135 0.0 106-107 5.144118921741233E-4 6.430148652176541E-5 0.0 10.21885653952548 0.0 108-109 5.46562635435006E-4 6.430148652176541E-5 0.0 10.813323782419202 0.0 110-111 5.787133786958887E-4 6.430148652176541E-5 0.0 11.4477543991862 0.0 112-113 5.787133786958887E-4 6.430148652176541E-5 0.0 12.190661623715418 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1545 0.0 38.512398 5 TAACGCA 195 1.8189894E-12 36.6164 4 AAAGTAC 1735 0.0 31.89426 3 AAGTACT 1835 0.0 31.453192 4 ATAACGC 190 1.0768417E-9 31.316662 3 GCAACGC 100 4.438626E-4 29.750828 3 TGTACGG 110 7.751212E-4 27.046207 5 TATAACG 220 5.156835E-9 27.046207 2 GTTATCA 870 0.0 24.64991 1 GTGTTAT 390 0.0 24.439226 1 AAAAGTA 2400 0.0 23.304815 2 AAAAAGT 2420 0.0 22.64668 1 GTATAAC 440 3.6379788E-12 20.308165 1 GTAGTGC 150 0.004706752 19.833248 8 GAGTACT 10355 0.0 19.450493 12-13 GTGTAGC 530 0.0 19.107555 1 CATGGGG 4525 0.0 18.935333 4 AGTACTT 10620 0.0 18.572958 12-13 TTATCAA 1065 0.0 18.437132 2 CTATCCG 85 0.0096996045 17.498236 42-43 >>END_MODULE