##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364137_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1393832 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.413925781586304 31.0 31.0 34.0 26.0 34.0 2 30.481348541287616 31.0 31.0 34.0 26.0 34.0 3 30.613084647217168 33.0 31.0 34.0 26.0 34.0 4 33.89640286634257 37.0 35.0 37.0 28.0 37.0 5 33.94337696365129 37.0 35.0 37.0 30.0 37.0 6 33.79628750093268 37.0 35.0 37.0 28.0 37.0 7 33.7458904659959 37.0 35.0 37.0 28.0 37.0 8 33.64296773212266 37.0 35.0 37.0 28.0 37.0 9 34.984653100230155 39.0 35.0 39.0 27.0 39.0 10-11 34.943672910365095 39.0 35.0 39.0 27.0 39.0 12-13 34.824816046697165 39.0 35.0 39.0 26.0 39.0 14-15 36.001872535571 40.0 36.0 41.0 25.5 41.0 16-17 36.03391728701881 40.0 36.0 41.0 27.0 41.0 18-19 35.95075339065254 40.0 36.0 41.0 26.0 41.0 20-21 35.991047342864846 40.0 36.5 41.0 27.0 41.0 22-23 36.02718943172491 40.0 36.0 41.0 27.0 41.0 24-25 36.04764419241343 40.0 36.0 41.0 27.0 41.0 26-27 35.84990371866911 40.0 36.0 41.0 26.0 41.0 28-29 35.72404098915794 40.0 36.0 41.0 25.5 41.0 30-31 35.790287136469814 40.0 36.0 41.0 27.0 41.0 32-33 35.44443770841823 39.0 35.0 41.0 26.0 41.0 34-35 35.495281353850395 39.5 35.0 41.0 26.5 41.0 36-37 35.515781313673386 40.0 35.0 41.0 26.5 41.0 38-39 35.349530287724775 40.0 35.0 41.0 25.5 41.0 40-41 34.947033788864076 39.0 34.0 41.0 24.5 41.0 42-43 34.67750130575277 39.0 33.5 41.0 24.0 41.0 44-45 34.3821396696302 38.0 33.0 40.5 23.0 41.0 46-47 34.088221177301136 38.0 33.0 40.0 22.5 41.0 48-49 33.77730242956109 38.0 33.0 40.0 19.5 41.0 50-51 33.66910000631353 38.0 33.0 40.0 18.5 41.0 52-53 33.516453202394544 38.0 33.0 40.0 16.5 41.0 54-55 33.221456029134075 38.0 32.0 40.0 13.5 41.0 56-57 32.79873363504353 38.0 31.0 40.0 8.0 41.0 58-59 32.341507800079206 37.0 31.0 40.0 7.5 41.0 60-61 32.06335160908918 36.0 31.0 40.0 4.0 41.0 62-63 31.422394521003966 36.0 29.5 39.0 2.0 41.0 64-65 31.189457552990604 36.0 30.0 39.0 2.0 41.0 66-67 30.737478763581265 35.0 29.5 38.5 2.0 40.5 68-69 30.308602830183265 35.0 29.0 38.0 2.0 40.0 70-71 29.60042350871554 34.5 28.0 37.0 2.0 39.5 72-73 29.35443403509175 34.0 28.0 37.0 2.0 39.0 74-75 29.001442426346934 34.0 28.0 36.0 2.0 39.0 76-77 28.40635420911559 34.0 26.5 36.0 2.0 38.0 78-79 28.042724661221726 34.0 26.0 35.0 2.0 37.0 80-81 27.795927701473346 34.0 26.0 35.0 2.0 37.0 82-83 27.597129711471684 34.0 26.0 35.0 2.0 36.0 84-85 27.34552621836778 34.0 26.0 35.0 2.0 36.0 86-87 26.957096335856832 33.0 25.5 35.0 2.0 36.0 88-89 26.569327221645075 33.0 25.0 35.0 2.0 35.0 90-91 26.44037911312124 33.0 24.0 35.0 2.0 35.0 92-93 26.032132997377012 33.0 22.0 35.0 2.0 35.0 94-95 25.966264226965663 33.0 23.0 35.0 2.0 35.0 96-97 25.872204469405208 33.0 22.0 35.0 2.0 35.0 98-99 25.63282770089939 33.0 20.0 35.0 2.0 35.0 100-101 25.01977856728788 32.0 18.0 35.0 2.0 35.0 102-103 23.731918193871287 30.0 15.0 34.0 2.0 35.0 104-105 24.515398197200238 31.5 16.0 35.0 2.0 35.0 106-107 24.518964624144086 32.0 13.5 35.0 2.0 35.0 108-109 24.488233876105586 32.0 10.0 35.0 2.0 35.0 110-111 24.48059701599619 32.0 8.5 35.0 2.0 35.0 112-113 24.373707519988063 32.0 7.0 35.0 2.0 35.0 114-115 24.215385354906473 32.0 2.0 35.0 2.0 35.0 116-117 23.799636182839826 32.0 2.0 35.0 2.0 35.0 118-119 23.48249824943035 32.0 2.0 35.0 2.0 35.0 120-121 23.289927337010486 32.0 2.0 35.0 2.0 35.0 122-123 22.698838167010084 31.0 2.0 35.0 2.0 35.0 124-125 21.64110595825035 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4394662402619183 1101 2 0.36581216449959797 1101 3 0.4457645555454768 1101 4 0.4819335492719077 1101 5 0.23828592831886652 1101 6 0.2708704391429535 1101 7 0.26907292980068576 1101 8 0.3489780025253708 1101 9 0.4542790744322218 1101 10-11 0.36528562538677534 1101 12-13 0.37412166813633263 1101 14-15 0.5032195983620014 1101 16-17 0.5266774971667303 1101 18-19 0.4967318654268169 1101 20-21 0.560199681127024 1101 22-23 0.5024891221670202 1101 24-25 0.6185663667847621 1101 26-27 0.6546389895130176 1101 28-29 0.753222485027031 1101 30-31 0.5613532012239304 1101 32-33 0.6442704368520253 1101 34-35 0.5196143808296512 1101 36-37 0.4894051914602855 1101 38-39 0.49380772274464846 1101 40-41 0.6208276778246642 1101 42-43 0.6210965627275229 1101 44-45 0.6045836583648807 1101 46-47 0.6671239436262013 1101 48-49 0.5566654248237342 1101 50-51 0.5974980947120372 1101 52-53 0.6627639254401174 1101 54-55 0.6806406778172658 1101 56-57 0.6238346856204586 1101 58-59 0.6387428663106363 1101 60-61 0.5406395510579145 1101 62-63 0.39289571129958034 1101 64-65 0.5237950253220411 1101 66-67 0.7590376290975236 1101 68-69 0.5460714137917613 1101 70-71 0.5230250739114517 1101 72-73 0.5513301768820078 1101 74-75 0.4755262819752524 1101 76-77 0.6307416556695706 1101 78-79 0.6206996882714861 1101 80-81 0.5176315242085892 1101 82-83 0.4648213908058807 1101 84-85 0.5078859173410777 1101 86-87 0.5744728041097851 1101 88-89 0.47497377978583444 1101 90-91 0.5288363789967718 1101 92-93 0.6988439695675126 1101 94-95 0.5992263589062325 1101 96-97 0.6623858860964909 1101 98-99 0.5728634848321974 1101 100-101 0.6623482297093588 1101 102-103 0.5545600613450681 1101 104-105 0.7476455871490835 1101 106-107 0.7954208076436444 1101 108-109 0.7883935714737582 1101 110-111 0.7499201662999972 1101 112-113 0.5972319427955419 1101 114-115 0.7484376028619693 1101 116-117 0.7470848099985261 1101 118-119 0.7317968317530728 1101 120-121 0.7861179400867044 1101 122-123 0.7633246515306276 1101 124-125 0.6744390629984984 1102 1 0.3640578290441141 1102 2 0.4839506953895025 1102 3 0.48998720518016725 1102 4 0.5367218757363332 1102 5 0.5353692799257601 1102 6 0.5378656156257051 1102 7 0.5791844919593743 1102 8 0.6068721234254539 1102 9 0.7312393040435552 1102 10-11 0.6860352159612333 1102 12-13 0.6802287986724238 1102 14-15 0.8136984298358101 1102 16-17 0.6680650434535806 1102 18-19 0.7280118599670331 1102 20-21 0.703453241657634 1102 22-23 0.7786949956148348 1102 24-25 0.8919703459109201 1102 26-27 0.9060075133131704 1102 28-29 0.994737356033653 1102 30-31 0.8517199334721681 1102 32-33 1.0239003813558938 1102 34-35 1.0455066330184337 1102 36-37 0.9947132415299293 1102 38-39 0.9502345956950293 1102 40-41 1.0536116472460932 1102 42-43 1.130282986145886 1102 44-45 1.0494737953544657 1102 46-47 0.9421190713273404 1102 48-49 1.017716349786582 1102 50-51 1.052655941950988 1102 52-53 1.004155529044425 1102 54-55 0.9375420458368353 1102 56-57 0.9394688900731367 1102 58-59 0.9612604388340174 1102 60-61 1.0250300188478505 1102 62-63 0.9325748689535658 1102 64-65 0.9298872399395997 1102 66-67 0.9015674392408641 1102 68-69 0.9559845426378573 1102 70-71 0.9395546790781744 1102 72-73 1.0084452693759474 1102 74-75 0.9639230576039566 1102 76-77 0.9026509940899494 1102 78-79 1.017604658626098 1102 80-81 0.8613132841744005 1102 82-83 0.7840979171605014 1102 84-85 0.8672238803941603 1102 86-87 0.8154515463460612 1102 88-89 0.8365947461262806 1102 90-91 0.9095330696563337 1102 92-93 0.9217995447555865 1102 94-95 0.848142081855709 1102 96-97 0.8650085548685844 1102 98-99 0.8696580164125507 1102 100-101 0.8103243436013088 1102 102-103 0.5785208662511856 1102 104-105 0.703120632184131 1102 106-107 0.6043772069596685 1102 108-109 0.6303250562807428 1102 110-111 0.6697187263509967 1102 112-113 0.5505589548438969 1102 114-115 0.5471250020300076 1102 116-117 0.5141427224594395 1102 118-119 0.7094964781566837 1102 120-121 0.5199227352730844 1102 122-123 0.48700303899787656 1102 124-125 0.5505899164071479 1103 1 0.36531042965735594 1103 2 0.3709667825765628 1103 3 0.36761872528696315 1103 4 0.4523317542927572 1103 5 0.45986684961084023 1103 6 0.36373614820811184 1103 7 0.4748485867803467 1103 8 0.42187266643988863 1103 9 0.6992499570639552 1103 10-11 0.5738560055823001 1103 12-13 0.5505334096575965 1103 14-15 0.5737648408558513 1103 16-17 0.5638854175917061 1103 18-19 0.5628343226404056 1103 20-21 0.5317627253396324 1103 22-23 0.5954322383839354 1103 24-25 0.6217697949464664 1103 26-27 0.7583598778630147 1103 28-29 0.8718583952520689 1103 30-31 0.7045681092394318 1103 32-33 0.8260670917555331 1103 34-35 0.6743867905612433 1103 36-37 0.8225602756655448 1103 38-39 0.9086088128492236 1103 40-41 0.8666411306820194 1103 42-43 0.8773470999427815 1103 44-45 0.7903386718322807 1103 46-47 0.7484904170117446 1103 48-49 0.6754754108519236 1103 50-51 0.6955528517848037 1103 52-53 0.7684517740582066 1103 54-55 0.9479828292256514 1103 56-57 0.8814822834827183 1103 58-59 0.9147891690296603 1103 60-61 0.9255618417413913 1103 62-63 0.9240313235733026 1103 64-65 0.9432405445314167 1103 66-67 0.9579312726340987 1103 68-69 1.0714905741203893 1103 70-71 1.0137517452859477 1103 72-73 1.0016331000794274 1103 74-75 0.913222072109658 1103 76-77 0.7983396961008999 1103 78-79 0.7564185021579668 1103 80-81 0.8266780727525038 1103 82-83 0.8789285853853102 1103 84-85 0.9563824730237869 1103 86-87 0.6852518282403537 1103 88-89 0.6959554276697268 1103 90-91 0.6037522965315496 1103 92-93 0.5068980693416023 1103 94-95 0.5163058436514838 1103 96-97 0.6042136290604461 1103 98-99 0.6015172528751052 1103 100-101 0.5221968632143046 1103 102-103 0.3422854539967588 1103 104-105 0.4789776426424268 1103 106-107 0.45755580523475103 1103 108-109 0.5697530384887983 1103 110-111 0.6587569330357717 1103 112-113 0.43563012759181774 1103 114-115 0.47597189254481265 1103 116-117 0.5199331018526188 1103 118-119 0.2559347412013935 1103 120-121 0.16918462604697382 1103 122-123 0.11881452804756165 1103 124-125 0.07204324544052909 1104 1 0.10750178007628008 1104 2 0.13785375737942118 1104 3 0.14201449829706547 1104 4 0.16533909511643685 1104 5 0.09658704351793546 1104 6 0.13020679646464117 1104 7 0.037125262482661014 1104 8 -0.007952967339065253 1104 9 0.05513503361028427 1104 10-11 0.0694769963335915 1104 12-13 0.1889562106833509 1104 14-15 0.15678035419370673 1104 16-17 0.11818968058301493 1104 18-19 0.05411516930902138 1104 20-21 0.00987140918943652 1104 22-23 0.16505743860571442 1104 24-25 0.12506322670934367 1104 26-27 0.23542377460248076 1104 28-29 0.25833011681287843 1104 30-31 0.12170793624011367 1104 32-33 0.011149630298227464 1104 34-35 -0.08783552203887268 1104 36-37 0.04943742708712051 1104 38-39 0.15865333331804976 1104 40-41 0.15592274358400715 1104 42-43 0.1568107462124999 1104 44-45 -0.01091918783534851 1104 46-47 0.11155231345858141 1104 48-49 0.24372962128501996 1104 50-51 0.27848895023231535 1104 52-53 0.31987002773921347 1104 54-55 0.37352703781054686 1104 56-57 0.4072882724243172 1104 58-59 0.3084043803021501 1104 60-61 0.09085094082036704 1104 62-63 0.15492169444564752 1104 64-65 0.2683003704154707 1104 66-67 0.16525900489136802 1104 68-69 0.33346357209507005 1104 70-71 0.2682864558499567 1104 72-73 0.2408059627118817 1104 74-75 0.19987565376934313 1104 76-77 0.026207247465489303 1104 78-79 0.2734266448313889 1104 80-81 0.29089119632784843 1104 82-83 0.2658000285608253 1104 84-85 0.212041746565248 1104 86-87 0.25144028891987347 1104 88-89 0.1751318808178297 1104 90-91 -0.024630537145753806 1104 92-93 -0.011972593161480916 1104 94-95 -0.09222747883650229 1104 96-97 -0.10401137113207426 1104 98-99 -0.13404945178236005 1104 100-101 -0.1177931786397508 1104 102-103 -0.2419838520037345 1104 104-105 -0.14166031401725832 1104 106-107 -0.08048717602910926 1104 108-109 -0.014256123583034963 1104 110-111 -0.04147604137434158 1104 112-113 3.0513298240109066E-4 1104 114-115 0.11732955507099518 1104 116-117 0.07736342370913718 1104 118-119 0.041734011161963736 1104 120-121 0.030686019898624295 1104 122-123 -0.008398139253142034 1104 124-125 0.030715134879603312 1105 1 0.11685725821179105 1105 2 0.13649942803055026 1105 3 0.031673259860017566 1105 4 0.0575678444406833 1105 5 0.026956400948257908 1105 6 0.08120644732360205 1105 7 0.0658148948709325 1105 8 0.0636261074747253 1105 9 0.20526521065858105 1105 10-11 0.11672894195018557 1105 12-13 0.11754255551456083 1105 14-15 0.06436450469226429 1105 16-17 0.16587805249705667 1105 18-19 0.11504720224841236 1105 20-21 0.15416497110378202 1105 22-23 0.10169960282964752 1105 24-25 0.21990675614981825 1105 26-27 0.19149922754728266 1105 28-29 0.1002453665801184 1105 30-31 3.829506938046734E-4 1105 32-33 -0.11160077984863648 1105 34-35 0.06504848148257025 1105 36-37 0.08222817616358924 1105 38-39 0.04307709105170687 1105 40-41 -0.07391541734373419 1105 42-43 0.016987972831316256 1105 44-45 -0.021891203054899222 1105 46-47 0.05921248190974637 1105 48-49 0.19056937868831625 1105 50-51 0.12981681243797993 1105 52-53 0.2348985315384695 1105 54-55 0.14415005746756293 1105 56-57 0.22880352575805318 1105 58-59 0.17976667325775963 1105 60-61 0.1566027860119803 1105 62-63 0.17876464855424956 1105 64-65 0.2705015075640773 1105 66-67 0.20714278973039058 1105 68-69 0.11175654929203205 1105 70-71 0.18090135797784157 1105 72-73 0.25031043584679225 1105 74-75 0.1539959872928911 1105 76-77 -0.012189585111997303 1105 78-79 0.12319052881895232 1105 80-81 0.10538620417413469 1105 82-83 0.22683220491083844 1105 84-85 0.22474257305341538 1105 86-87 0.20931177686136948 1105 88-89 0.2663764184499797 1105 90-91 0.26333503116575585 1105 92-93 0.19087717670576865 1105 94-95 0.19734571032285686 1105 96-97 0.2559592569088309 1105 98-99 0.23780264756891256 1105 100-101 0.13505051410862734 1105 102-103 -0.016052996946068987 1105 104-105 0.23027484711320767 1105 106-107 0.1590802410405061 1105 108-109 0.15499445268738654 1105 110-111 0.14035710393590506 1105 112-113 0.32586835754391075 1105 114-115 0.11469633242413124 1105 116-117 0.20165493488915942 1105 118-119 0.27578802665932045 1105 120-121 0.2526073843267831 1105 122-123 0.12416013069670484 1105 124-125 0.24518170462521738 1106 1 0.24276426851291433 1106 2 0.34649484491411187 1106 3 0.39709966972398547 1106 4 0.33562187364904617 1106 5 0.24387272373301272 1106 6 0.33234077975557597 1106 7 0.31471834758904293 1106 8 0.347876234499509 1106 9 0.46843055714173687 1106 10-11 0.44838076201641286 1106 12-13 0.5026283566997733 1106 14-15 0.5516142391960202 1106 16-17 0.5520935185368785 1106 18-19 0.5595976056382312 1106 20-21 0.4913677631151998 1106 22-23 0.64476011552523 1106 24-25 0.6255693498736576 1106 26-27 0.7434144678986172 1106 28-29 0.8274359162029086 1106 30-31 0.6789513469275619 1106 32-33 0.44255334177776007 1106 34-35 0.5776157112665175 1106 36-37 0.60286131302896 1106 38-39 0.4646394536637928 1106 40-41 0.455744369610251 1106 42-43 0.4899238034222577 1106 44-45 0.5418036689668 1106 46-47 0.5060553325815036 1106 48-49 0.5052591114850671 1106 50-51 0.6557618746078901 1106 52-53 0.6154138854230666 1106 54-55 0.6589457220438959 1106 56-57 0.7237369816634498 1106 58-59 0.8136641314729474 1106 60-61 0.7623261916008985 1106 62-63 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1.568070884359063 2203 88-89 1.6082241527772894 2203 90-91 1.560928026440152 2203 92-93 1.7027976318626514 2203 94-95 1.6487261212479574 2203 96-97 1.6174826186724616 2203 98-99 1.8371287576770996 2203 100-101 1.8914794519707705 2203 102-103 1.9698359477066916 2203 104-105 1.8977603845831297 2203 106-107 1.839345149320792 2203 108-109 1.982329678178548 2203 110-111 1.9376683164321271 2203 112-113 2.038760580617538 2203 114-115 1.9918101671004358 2203 116-117 2.0903594345078815 2203 118-119 2.0866634271851296 2203 120-121 2.0794990628522925 2203 122-123 2.1452228715456023 2203 124-125 2.0068883295586595 2204 1 0.2538263710291204 2204 2 0.363587260146506 2204 3 0.32281766690871194 2204 4 0.2981276275093663 2204 5 0.1770834267590331 2204 6 0.28893908863258844 2204 7 0.3235033904903588 2204 8 0.2542554737701721 2204 9 0.2806232208696713 2204 10-11 0.28549821377524154 2204 12-13 0.3306738515940779 2204 14-15 0.2814993222519391 2204 16-17 0.2530612800025267 2204 18-19 0.29757459731973057 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1.131648778904232 2204 88-89 1.184135241922256 2204 90-91 1.2853293664574679 2204 92-93 1.1849739336972789 2204 94-95 1.04146829797714 2204 96-97 1.247549953985228 2204 98-99 1.2891827727123442 2204 100-101 1.2857275688488201 2204 102-103 1.2687179195313973 2204 104-105 1.2330868512257638 2204 106-107 1.2419337684637917 2204 108-109 1.2363609248108531 2204 110-111 1.184153836738627 2204 112-113 1.3570060999578608 2204 114-115 1.3072500484089389 2204 116-117 1.2599474795170025 2204 118-119 1.413602243837616 2204 120-121 1.5251161359958338 2204 122-123 1.3426622158810027 2204 124-125 1.3230219559665848 2205 1 0.14136470655393651 2205 2 0.13169404600267853 2205 3 0.15421050157073068 2205 4 0.14214256813121295 2205 5 0.1283499617363404 2205 6 0.13454618783297434 2205 7 0.20276828266526792 2205 8 0.329363733616006 2205 9 0.22112297135055314 2205 10-11 0.2101936223922749 2205 12-13 0.24056033542806432 2205 14-15 0.26378785884892153 2205 16-17 0.27303807171858807 2205 18-19 0.3577189946559116 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-3.041716637772712 2216 106-107 -3.1093499048879423 2216 108-109 -3.025488529188447 2216 110-111 -3.0822699932721527 2216 112-113 -3.238505653287927 2216 114-115 -3.3905547054221117 2216 116-117 -3.4833297807174297 2216 118-119 -3.529751448477068 2216 120-121 -3.5665386987150214 2216 122-123 -3.4853634083365925 2216 124-125 -3.5666520394497816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48525.0 3 7326.0 4 4688.0 5 3297.0 6 3830.0 7 3966.0 8 4892.0 9 6040.0 10 7924.0 11 9730.0 12 11174.0 13 12505.0 14 12920.0 15 12572.0 16 12451.0 17 12686.0 18 12743.0 19 12984.0 20 13429.0 21 14451.0 22 15362.0 23 17901.0 24 20355.0 25 22529.0 26 25408.0 27 27247.0 28 31299.0 29 38694.0 30 45883.0 31 55016.0 32 69195.0 33 85818.0 34 103242.0 35 122584.0 36 158878.0 37 228234.0 38 97463.0 39 591.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.756814916436035 16.09706583541255 9.175635353579773 22.970483894571643 2 12.38705603606422 23.340611243542327 36.30994815119696 27.96238456919649 3 23.371373301967296 32.3625141262483 25.91135923337715 18.35475333840725 4 13.964187000219821 18.757464044656338 32.07508549641978 35.20326345870406 5 11.336503644079524 42.25597063978966 31.730015222104342 14.677510494026476 6 30.737610684849553 27.171477275594015 22.331004011143264 19.759908028413165 7 25.698391661459763 32.8847829424012 21.43954118714511 19.977284208993925 8 30.638068723300265 31.816641748114527 22.157158448830945 15.388131079754267 9 33.20062810284391 15.829676850026747 18.980356645907744 31.9893384012216 10-11 23.96738756218206 30.780367502778567 23.369423171832118 21.882821763207254 12-13 27.773668025768867 27.374228970867627 22.709480188471783 22.142622814891723 14-15 29.203948075014292 25.033355943052342 22.445864619922453 23.316831362010916 16-17 24.0062971874581 28.12963585014193 29.828059967447228 18.036006994952736 18-19 26.712408985338556 26.906033049065485 28.201544884349893 18.18001308124607 20-21 21.909484894627298 28.424734428356473 29.434113905232145 20.23166677178408 22-23 20.639756612764852 31.146176527838858 21.576682118680115 26.637384740716175 24-25 21.21610765250092 32.93552808610528 20.58224069644271 25.266123564951087 26-27 24.653585545500487 26.267122472946113 21.651435076081054 27.427856905472343 28-29 23.961194723837707 27.052785051714533 25.26931387903178 23.71670634541598 30-31 27.5132334259541 28.87658992168811 23.826907797880057 19.783268854477736 32-33 19.650989214759978 29.321621055568553 27.32188709076811 23.705502638903358 34-35 17.153901664824712 31.044514758350616 27.935899959123734 23.865683617700935 36-37 25.008656188445133 27.875845216901922 24.560480005015364 22.55501858963758 38-39 28.364131299968808 25.881349131742976 23.690483136573548 22.06403643171467 40-41 22.53746953169568 30.9521597201814 23.682551961784245 22.827818786338675 42-43 26.264804040284712 28.51704233986136 22.700200760830963 22.517952859022962 44-45 28.41020863012307 25.776156948536748 23.286798144068854 22.52683627727133 46-47 23.6413393026042 27.561276607244196 30.236999270355724 18.560384819795882 48-49 26.308803277944282 26.72690547445808 28.106959794595227 18.857331453002416 50-51 20.912614237995463 28.68267127413372 29.401423165491263 21.00329132237955 52-53 19.993241911818476 31.210299384026918 21.97639496342311 26.82006374073149 54-55 20.803755764361235 32.55835149265479 20.95037524861369 25.687517494370283 56-57 24.250100582097502 26.336337169200156 21.828962529431745 27.584599719270592 58-59 23.737157434019025 26.877041672174762 25.22717064264069 24.158630251165526 60-61 26.838839223959056 28.706929842762946 24.064063436621016 20.390167496656982 62-63 19.435796754264306 29.299411012446193 27.127073614438302 24.137718618851203 64-65 16.973648181241714 30.649211679252897 27.99929382602179 24.3778463134836 66-67 24.246277816184335 27.986708249020033 24.60767971837166 23.159334216423975 68-69 27.46732195088034 26.149428289501415 23.799253042283773 22.583996717334472 70-71 22.195520817307987 30.44267606684222 23.751968528050828 23.609834587798964 72-73 25.73152110705101 28.378954847347416 22.91294814157213 22.976575904029445 74-75 27.82355100909888 25.59795949912751 23.447528407647628 23.130961084125985 76-77 23.330914682388137 27.32111230082157 29.965319859982447 19.38265315680784 78-79 25.75589680926025 26.769150850820704 27.94653136919523 19.528420970723815 80-81 20.60403107701342 28.685711815281635 29.083235309482163 21.62702179822278 82-83 19.807379915070776 30.917602368824266 22.173394103085936 27.101623613019022 84-85 20.633325191424916 32.12263875649281 21.326143956146097 25.91789209593618 86-87 23.67265118129073 26.15715187447219 22.3086617418598 27.86153520237728 88-89 23.388736728383833 26.403964346530167 25.47754042500152 24.729758500084476 90-91 26.018612952115944 28.36189377583256 24.311326896050844 21.308166376000653 92-93 19.571894420511484 28.78439329912303 26.941965580490113 24.701746699875375 94-95 17.364622044166154 30.00275610621637 27.895073415103823 24.737548434513656 96-97 24.047372246421546 27.496958376763654 24.88926618543349 23.566403191381312 98-99 26.801568643266165 26.087570219687112 23.971644587291337 23.139216549755382 100-101 22.063840252457346 29.41341258873203 24.056111554806275 24.466635604004345 102-103 24.99739890188658 27.966934912784907 23.515691011864966 23.519975173463543 104-105 26.7845821577246 25.554184687066968 23.916930889867256 23.744302265341176 106-107 22.879166732682393 27.140133865492118 29.666711797418614 20.313987604406876 108-109 24.905310055103463 27.059357757661438 27.62839769272708 20.406934494508018 110-111 20.194500241135348 28.946182695954437 28.855959536804026 22.00335752610619 112-113 19.554799380237174 30.637742000312617 22.59353887888045 27.213919740569757 114-115 20.02316465488554 32.48608752148277 21.84323953664928 25.64750828698241 116-117 22.79315025582124 27.09562781520381 22.551011223009148 27.560210705965805 118-119 22.507437755078875 27.429417442810266 25.121506453020448 24.941638349090407 120-121 24.80127758872424 29.100816221225788 24.313209581338903 21.78469660871107 122-123 19.515210015631414 29.640585493758238 26.213703781073743 24.630500709536605 124-125 17.878821274453237 30.766440997095035 26.925088218578953 24.42964950987277 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6305.0 1 3665.5 2 1603.0 3 2142.5 4 1983.5 5 1678.5 6 1363.5 7 1151.5 8 1058.5 9 995.0 10 894.5 11 840.5 12 860.5 13 915.0 14 945.0 15 1000.5 16 1121.0 17 1241.5 18 1351.0 19 1494.5 20 1736.0 21 2148.5 22 2605.5 23 3264.0 24 3972.0 25 4639.5 26 5584.5 27 6604.5 28 7506.5 29 8471.0 30 9848.5 31 11334.5 32 12597.0 33 13926.0 34 15681.0 35 16838.5 36 18024.5 37 19538.0 38 19947.5 39 19904.0 40 20014.0 41 21402.0 42 23025.5 43 23976.5 44 25358.5 45 27240.0 46 30918.0 47 39901.5 48 54192.5 49 95118.0 50 229238.5 51 244836.0 52 106713.5 53 43779.5 54 29750.5 55 23365.0 56 18826.5 57 15721.0 58 14021.5 59 12697.0 60 11041.5 61 9340.5 62 7849.5 63 6341.5 64 5065.5 65 4085.0 66 3020.0 67 2438.0 68 2023.5 69 1461.0 70 1165.0 71 1034.0 72 998.0 73 924.5 74 687.5 75 569.5 76 453.0 77 305.5 78 221.0 79 180.5 80 162.0 81 124.5 82 95.5 83 86.0 84 84.0 85 68.0 86 50.0 87 42.0 88 38.0 89 30.5 90 26.0 91 25.0 92 17.0 93 12.5 94 10.0 95 9.0 96 9.0 97 4.5 98 2.5 99 3.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4800435059605462 2 0.4684208713819169 3 0.4551481096717538 4 0.4553633436454322 5 0.4574439387243226 6 0.46253780943470946 7 0.44847585648772587 8 0.47681499635537133 9 0.48772018435507297 10-11 0.49335213999965566 12-13 0.49410545890752977 14-15 0.4679904034345602 16-17 0.46964053056609406 18-19 0.4555427052901641 20-21 0.4659456806846162 22-23 0.4837024835130776 24-25 0.4701786155002899 26-27 0.48018699527633174 28-29 0.4770661026579961 30-31 0.4888680988813573 32-33 0.4876843120261265 34-35 0.4820523563815438 36-37 0.4373913068432924 38-39 0.4011243822784955 40-41 0.39488259704182427 42-43 0.4027027647521365 44-45 0.43513135011967013 46-47 0.4426286668694649 48-49 0.4400099868563787 50-51 0.4261632678830734 52-53 0.4208182908700618 54-55 0.4222531840279172 56-57 0.40625412531782884 58-59 0.410630549449288 60-61 0.4207106738832227 62-63 0.42020846127797323 64-65 0.4356335627249195 66-67 0.4235445878699872 68-69 0.4258404169225559 70-71 0.4345215205275815 72-73 0.43570530738281227 74-75 0.4400458591853251 76-77 0.4308984153039964 78-79 0.42171509909372146 80-81 0.42971462844876573 82-83 0.428351479948803 84-85 0.4269524591198939 86-87 0.4324767977776375 88-89 0.4204595675805979 90-91 0.42526645965941373 92-93 0.43358883997497544 94-95 0.43079079831715733 96-97 0.40126787159428107 98-99 0.3761931136607568 100-101 0.3760137520160249 102-103 0.358400438503349 104-105 0.35962009768752623 106-107 0.3783813257264864 108-109 0.3840491537000155 110-111 0.4023081691337263 112-113 0.3988285532259268 114-115 0.3953130649891809 116-117 0.3707046473319596 118-119 0.44112202905371667 120-121 0.4461082827772644 122-123 0.4019135735153161 124-125 0.39595876691021586 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1393832.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.72877635992734 #Duplication Level Percentage of deduplicated Percentage of total 1 89.0623192314222 44.28960155155849 2 7.552943528580923 7.511972791839224 3 1.6199050588411024 2.4166768918627235 4 0.5986698608383338 1.1908447849223327 5 0.30076407382738674 0.7478314682231394 6 0.18381838634723766 0.54846380553027 7 0.1210276781444771 0.4212990837865576 8 0.07802083507369904 0.31039045270358034 9 0.0651549126629866 0.29160666725114326 >10 0.36126979348526683 3.269120748122799 >50 0.027358184072915556 0.9080451336706791 >100 0.0200538027230001 1.9726042830499237 >500 0.0026644907359841897 0.983651995069412 >1k 0.0022437816724077387 2.5484292701752236 >5k 0.0022437816724077387 7.941882772243517 >10k+ 0.00154259989978032 24.647578299990958 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 90800 6.514414936663816 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 61558 4.4164576505633395 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 42578 3.054744043758502 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 31272 2.243598941622807 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22577 1.619779141245143 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 21734 1.559298394641535 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 21166 1.5185474289584397 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 18372 1.3180928548060311 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 17033 1.2220267578876076 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13719 0.984264961630957 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 12623 0.9056328165804773 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9553 0.6853767168496634 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9220 0.6614857457713699 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9072 0.6508675364032394 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8927 0.6404645610087873 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8315 0.59655683037841 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8238 0.5910324917206664 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7503 0.538300168169478 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 7316 0.5248839171435294 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 6862 0.4923118424602104 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6315 0.45306751459286343 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5730 0.41109688972559105 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 5730 0.41109688972559105 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 5433 0.38978872633143735 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5419 0.38878430112093854 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5131 0.36812183964781986 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 5118 0.36718915909521377 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4790 0.34365691130638415 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4323 0.310152156070459 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4315 0.3095781988073168 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3229 0.23166350033576502 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 2931 0.21028359228371854 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2775 0.19909142565244592 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 1925 0.13810846644358862 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1757 0.1260553639176027 No Hit ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG 1605 0.11515017591790117 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1590 0.11407400604950955 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1467 0.10524941312869843 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.174465789277331E-5 0.0 0.0 5.022126052494131E-4 0.0 12-13 7.174465789277331E-5 0.0 0.0 0.0013631484999626928 0.0 14-15 7.174465789277331E-5 0.0 0.0 0.0035154882367458918 0.0 16-17 7.174465789277331E-5 0.0 0.0 0.005560210986689931 0.0 18-19 1.0761698683915997E-4 0.0 0.0 0.006564636197188757 0.0 20-21 1.4348931578554662E-4 0.0 0.0 0.008394124973454476 0.0 22-23 2.1523397367831991E-4 0.0 0.0 0.013272761710163061 0.0 24-25 2.1523397367831991E-4 0.0 0.0 0.018617738723174675 0.0 26-27 2.1523397367831991E-4 0.0 0.0 0.023496375459883256 0.0 28-29 2.5110630262470656E-4 0.0 0.0 0.03131654317019555 0.0 30-31 2.8697863157109324E-4 0.0 0.0 0.04286743309093205 0.0 32-33 2.8697863157109324E-4 0.0 0.0 0.061879767432516976 0.0 34-35 3.2285096051747986E-4 0.0 0.0 0.09696290514208313 0.0 36-37 3.5872328946386653E-4 0.0 0.0 0.1405477848119429 0.0 38-39 3.5872328946386653E-4 0.0 0.0 0.2107140602310752 0.0 40-41 3.5872328946386653E-4 0.0 0.0 0.329451469043615 0.0 42-43 3.5872328946386653E-4 0.0 0.0 0.4113838683571621 0.0 44-45 3.5872328946386653E-4 0.0 0.0 0.49622192631536655 0.0 46-47 4.3046794735663983E-4 0.0 0.0 0.6089327838649135 0.0 48-49 5.022126052494131E-4 0.0 0.0 0.7066131355859243 0.0 50-51 5.022126052494131E-4 0.0 0.0 0.8102482939120353 0.0 52-53 5.022126052494131E-4 0.0 0.0 0.9458098250004305 0.0 54-55 5.022126052494131E-4 0.0 0.0 1.078788548404686 0.0 56-57 5.022126052494131E-4 0.0 0.0 1.2323938609531133 0.0 58-59 5.022126052494131E-4 0.0 0.0 1.414553547342865 0.0 60-61 5.022126052494131E-4 0.0 0.0 1.5756561766410875 0.0 62-63 5.022126052494131E-4 0.0 0.0 1.7302300420710672 0.0 64-65 5.022126052494131E-4 0.0 0.0 1.9203175131579702 0.0 66-67 5.739572631421865E-4 3.5872328946386655E-5 0.0 2.125076766783945 0.0 68-69 5.739572631421865E-4 7.174465789277331E-5 0.0 2.3806312381980037 0.0 70-71 5.739572631421865E-4 7.174465789277331E-5 0.0 2.8048932726469187 0.0 72-73 5.739572631421865E-4 7.174465789277331E-5 0.0 3.1158704922831446 0.0 74-75 5.739572631421865E-4 7.174465789277331E-5 0.0 3.41791550201172 0.0 76-77 5.739572631421865E-4 7.174465789277331E-5 0.0 3.770899218844165 0.0 78-79 5.739572631421865E-4 7.174465789277331E-5 0.0 4.084100522875067 0.0 80-81 5.739572631421865E-4 7.174465789277331E-5 0.0 4.410108248339829 0.0 82-83 5.739572631421865E-4 7.174465789277331E-5 0.0 4.841365386933289 0.0 84-85 5.739572631421865E-4 7.174465789277331E-5 0.0 5.251673085422059 0.0 86-87 5.739572631421865E-4 7.174465789277331E-5 0.0 5.670984738476372 0.0 88-89 5.739572631421865E-4 7.174465789277331E-5 0.0 6.1534675628052735 0.0 90-91 5.739572631421865E-4 7.174465789277331E-5 0.0 6.581424447135666 0.0 92-93 5.739572631421865E-4 7.174465789277331E-5 0.0 7.014654563821178 0.0 94-95 5.739572631421865E-4 7.174465789277331E-5 0.0 7.508724150399761 0.0 96-97 5.739572631421865E-4 7.174465789277331E-5 0.0 7.997663993939011 0.0 98-99 7.174465789277331E-4 7.174465789277331E-5 0.0 8.582454700423007 0.0 100-101 7.891912368205063E-4 7.174465789277331E-5 0.0 9.48503836904304 0.0 102-103 7.891912368205063E-4 7.174465789277331E-5 0.0 10.182755167050262 0.0 104-105 8.609358947132797E-4 7.174465789277331E-5 0.0 10.833371597150876 0.0 106-107 8.609358947132797E-4 7.174465789277331E-5 0.0 11.511466231224423 0.0 108-109 8.609358947132797E-4 7.174465789277331E-5 0.0 12.087504089445499 0.0 110-111 8.609358947132797E-4 7.174465789277331E-5 0.0 12.69091253465267 0.0 112-113 8.609358947132797E-4 7.174465789277331E-5 0.0 13.446706633224089 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 275 0.0 71.41673 2 ATAACGC 300 0.0 67.45158 3 AGTACTC 1450 0.0 57.87427 5 AAGTACT 1630 0.0 51.848377 4 GTATAAC 495 0.0 44.489983 1 AAAAAGT 1945 0.0 42.53642 1 AAAAGTA 2070 0.0 40.25098 2 AAAGTAC 2120 0.0 39.864555 3 TATCACG 130 4.1951353E-8 36.62397 2 TAACGCA 575 0.0 36.22719 4 GTACAAA 995 0.0 31.704325 1 ATCACGC 165 3.4005643E-7 28.856289 3 TCACGCA 200 6.1018E-8 26.782242 4 GTCGGGA 300 7.2759576E-12 25.789377 2 TACAACG 310 1.2732926E-11 24.957462 2 GGTATGC 120 0.0012867788 24.79569 8 TCTATAG 275 2.1118467E-9 23.806437 3 GTACAAC 380 0.0 23.494888 1 GTACATA 660 0.0 23.447424 1 GTATAGG 210 2.7825226E-6 22.674433 1 >>END_MODULE