##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364137_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1393832 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07375996533298 33.0 31.0 34.0 30.0 34.0 2 31.883720563166868 33.0 31.0 34.0 30.0 34.0 3 32.14166556658191 34.0 31.0 34.0 30.0 34.0 4 35.571982132710396 37.0 35.0 37.0 33.0 37.0 5 35.71215828019446 37.0 35.0 37.0 33.0 37.0 6 35.876647974791794 37.0 35.0 37.0 35.0 37.0 7 35.850496329543304 37.0 35.0 37.0 35.0 37.0 8 35.915532861923104 37.0 35.0 37.0 35.0 37.0 9 37.58004981949044 39.0 37.0 39.0 35.0 39.0 10-11 37.52552029225904 39.0 37.0 39.0 35.0 39.0 12-13 37.5164108730464 39.0 37.0 39.0 34.5 39.0 14-15 38.80509415768902 40.0 38.0 41.0 34.0 41.0 16-17 38.77037440667168 40.0 38.0 41.0 34.0 41.0 18-19 38.81558860752229 40.0 38.0 41.0 34.0 41.0 20-21 38.84479872753675 40.0 38.0 41.0 35.0 41.0 22-23 38.801551047758984 40.0 38.0 41.0 35.0 41.0 24-25 38.78017365076997 40.0 38.0 41.0 35.0 41.0 26-27 38.56649294893502 40.0 38.0 41.0 34.0 41.0 28-29 38.38242270230559 40.0 38.0 41.0 34.0 41.0 30-31 38.22761351439772 40.0 38.0 41.0 34.0 41.0 32-33 37.85368107490716 40.0 37.0 41.0 33.5 41.0 34-35 37.58132687440093 40.0 37.0 41.0 33.0 41.0 36-37 37.457919247082856 40.0 37.0 41.0 33.0 41.0 38-39 37.33658073569842 40.0 37.0 41.0 32.5 41.0 40-41 37.032746414202 40.0 36.0 41.0 31.0 41.0 42-43 36.845242468245814 40.0 36.0 41.0 31.0 41.0 44-45 36.57728083441907 40.0 36.0 41.0 30.5 41.0 46-47 36.21545171871502 39.0 35.0 41.0 30.0 41.0 48-49 35.847751737655614 39.0 35.0 41.0 28.5 41.0 50-51 35.69108292821517 39.0 35.0 41.0 28.0 41.0 52-53 35.59313461019693 39.0 35.0 41.0 27.5 41.0 54-55 35.404457997807484 39.0 35.0 41.0 27.0 41.0 56-57 35.09186114251933 39.0 35.0 41.0 26.0 41.0 58-59 34.89052805503103 38.5 35.0 40.0 25.5 41.0 60-61 34.712406875434056 38.0 35.0 40.0 26.0 41.0 62-63 34.316283813257265 38.0 34.0 40.0 25.0 41.0 64-65 34.038554861704995 37.5 34.0 40.0 23.0 41.0 66-67 33.530501165133245 37.0 34.0 39.0 22.5 41.0 68-69 33.20067913493161 36.0 33.0 39.0 22.0 41.0 70-71 32.64267931859794 36.0 33.0 39.0 21.0 40.0 72-73 32.295869947023746 35.0 33.0 37.5 20.5 39.0 74-75 31.96516007668069 35.0 32.0 37.0 20.0 39.0 76-77 31.442242321886713 35.0 32.0 36.5 18.0 38.0 78-79 31.01267010658386 35.0 31.5 36.0 15.0 37.0 80-81 30.777636042220298 35.0 31.5 35.5 12.5 37.0 82-83 30.58114679530962 35.0 32.0 35.0 9.0 36.5 84-85 30.34301228555522 35.0 31.0 35.0 7.0 36.0 86-87 30.049710797284035 34.0 31.0 35.0 6.0 36.0 88-89 29.82195451101711 34.0 31.0 35.0 2.0 35.5 90-91 29.643779881650012 34.0 31.0 35.0 2.0 35.0 92-93 29.42136103920702 34.0 30.0 35.0 2.0 35.0 94-95 29.339954886959116 34.0 30.0 35.0 2.0 35.0 96-97 29.232707385108107 34.0 30.0 35.0 2.0 35.0 98-99 29.1157119365892 34.0 30.0 35.0 2.0 35.0 100-101 28.782970257534622 34.0 29.0 35.0 2.0 35.0 102-103 27.293635100930388 32.5 26.0 34.5 2.0 35.0 104-105 28.214822518065304 34.0 29.0 35.0 2.0 35.0 106-107 28.284699662513127 34.0 29.0 35.0 2.0 35.0 108-109 28.19846545351233 34.0 29.0 35.0 2.0 35.0 110-111 28.19141797576752 34.0 29.0 35.0 2.0 35.0 112-113 28.12593770267866 34.0 29.0 35.0 2.0 35.0 114-115 28.022538584277015 34.0 29.0 35.0 2.0 35.0 116-117 27.727725794787318 34.0 29.0 35.0 2.0 35.0 118-119 27.49761843608125 34.0 27.0 35.0 2.0 35.0 120-121 27.28529442572706 34.0 27.0 35.0 2.0 35.0 122-123 26.906069382823755 34.0 26.0 35.0 2.0 35.0 124-125 25.773097833885288 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2927484876620241 1101 2 0.6599104271327043 1101 3 0.4088224185128624 1101 4 0.4948839633794222 1101 5 0.16160920131637369 1101 6 0.14809003079597716 1101 7 0.16337677673231354 1101 8 0.18417407319343937 1101 9 0.2548050533392683 1101 10-11 0.19856290582667668 1101 12-13 0.1991533044159013 1101 14-15 0.25297706362112393 1101 16-17 0.3374702600102566 1101 18-19 0.25853085961676214 1101 20-21 0.14270670418322595 1101 22-23 0.1718540743067436 1101 24-25 0.14462809829629464 1101 26-27 0.13727283147753155 1101 28-29 0.21017989969948303 1101 30-31 0.23608528928935613 1101 32-33 0.2934078642020239 1101 34-35 0.1737495550447079 1101 36-37 0.19108986242133597 1101 38-39 0.2727170996046553 1101 40-41 0.31181289423702907 1101 42-43 0.3246011079588911 1101 44-45 0.2938638871175385 1101 46-47 0.3399449186351191 1101 48-49 0.39802839829147985 1101 50-51 0.25581966683949275 1101 52-53 0.24519724490897232 1101 54-55 0.2557212089961993 1101 56-57 0.26946732248049443 1101 58-59 0.26448110097317823 1101 60-61 0.29692380536215524 1101 62-63 0.29249626606306833 1101 64-65 0.14523113249156694 1101 66-67 0.1758439438247521 1101 68-69 0.3332704847239043 1101 70-71 0.16515831369746792 1101 72-73 0.2582386472950873 1101 74-75 0.35659777653204827 1101 76-77 0.3755074288356681 1101 78-79 0.34222626380367416 1101 80-81 0.2623332489254686 1101 82-83 0.15066754566356977 1101 84-85 0.23048253587434075 1101 86-87 0.32610078465036096 1101 88-89 0.4271560918401569 1101 90-91 0.49154686401078607 1101 92-93 0.41219791656841664 1101 94-95 0.3280067530931312 1101 96-97 0.3513860722103743 1101 98-99 0.43463905718000007 1101 100-101 0.3422275099255039 1101 102-103 0.307021999083986 1101 104-105 0.33512246168657356 1101 106-107 0.41009856594682503 1101 108-109 0.38832710781190727 1101 110-111 0.23980770964715958 1101 112-113 0.2718984275010037 1101 114-115 0.2394190216030907 1101 116-117 0.24709933491599756 1101 118-119 0.24263556066338765 1101 120-121 0.22272885858806646 1101 122-123 0.26083036178927443 1101 124-125 0.3241794335775836 1102 1 0.2607779986661498 1102 2 0.5836560769496266 1102 3 0.4414188601281879 1102 4 0.4258422572047209 1102 5 0.32173275953219616 1102 6 0.2257768720242339 1102 7 0.22159998594847963 1102 8 0.24568675840821896 1102 9 0.31114876300794947 1102 10-11 0.2811430360876699 1102 12-13 0.19911437293038858 1102 14-15 0.34287455420545143 1102 16-17 0.396700176464698 1102 18-19 0.3242725360768901 1102 20-21 0.2992708457990858 1102 22-23 0.266631837674133 1102 24-25 0.27356483955740885 1102 26-27 0.3484682837171533 1102 28-29 0.4183644940416187 1102 30-31 0.3282569883977544 1102 32-33 0.4026183031873529 1102 34-35 0.469579125196546 1102 36-37 0.5050578696522692 1102 38-39 0.47862736815920215 1102 40-41 0.4253738094646593 1102 42-43 0.4872171020919609 1102 44-45 0.6149977502161406 1102 46-47 0.5767537704353884 1102 48-49 0.5778645389523476 1102 50-51 0.5994249709964166 1102 52-53 0.6343982371458878 1102 54-55 0.5749899959591218 1102 56-57 0.7152033984353565 1102 58-59 0.7087471615594865 1102 60-61 0.6281086757425953 1102 62-63 0.6407239805433065 1102 64-65 0.6262007891510422 1102 66-67 0.6023870319499238 1102 68-69 0.7480424271306347 1102 70-71 0.6714201851621198 1102 72-73 0.7514682039850769 1102 74-75 0.6882281309891525 1102 76-77 0.6866844472514302 1102 78-79 0.6782014658545634 1102 80-81 0.574621456367435 1102 82-83 0.7038580395329141 1102 84-85 0.6650878421420039 1102 86-87 0.7122898636181887 1102 88-89 0.7330726691715732 1102 90-91 0.7054869859789079 1102 92-93 0.7575695609851145 1102 94-95 0.8247984234438235 1102 96-97 0.8678137519337064 1102 98-99 0.924065992749096 1102 100-101 0.8661832725006668 1102 102-103 0.6058693456804463 1102 104-105 0.7506513400540591 1102 106-107 0.808814671222251 1102 108-109 0.8093334820218132 1102 110-111 0.8519417624781234 1102 112-113 0.7730557010868608 1102 114-115 0.8551558785329334 1102 116-117 0.9094533623664489 1102 118-119 0.8072285237739294 1102 120-121 0.7092053269676093 1102 122-123 0.7445797499669062 1102 124-125 0.6464661142486392 1103 1 0.22808474408844148 1103 2 0.5401070292597048 1103 3 0.28760546733378334 1103 4 0.40587801807678403 1103 5 0.13387697564768075 1103 6 0.15528034861794993 1103 7 0.2355110929478741 1103 8 0.15604915370048644 1103 9 0.26580457348715925 1103 10-11 0.25979641708008216 1103 12-13 0.20163755633416258 1103 14-15 0.3630666464483383 1103 16-17 0.352532873126421 1103 18-19 0.22577090100009656 1103 20-21 0.1766832672719829 1103 22-23 0.218324729532398 1103 24-25 0.1633379967568871 1103 26-27 0.19261533344521098 1103 28-29 0.1963434014208616 1103 30-31 0.19591928405456116 1103 32-33 0.2958757438455777 1103 34-35 0.33042451940491446 1103 36-37 0.28337784688115875 1103 38-39 0.31931367688432033 1103 40-41 0.30810481478430063 1103 42-43 0.2368167028876229 1103 44-45 0.22266580016968618 1103 46-47 0.1660235686351328 1103 48-49 0.4095861407763053 1103 50-51 0.41507795945682346 1103 52-53 0.34938510424127855 1103 54-55 0.33000980356401755 1103 56-57 0.2918999872873016 1103 58-59 0.18195123300834837 1103 60-61 0.271453602059438 1103 62-63 0.14911077339122158 1103 64-65 0.2867146724566467 1103 66-67 0.24238183131495816 1103 68-69 0.3186154930942351 1103 70-71 0.2548469587785789 1103 72-73 0.2598657624786114 1103 74-75 0.4153000859617819 1103 76-77 0.3052310046464015 1103 78-79 0.42087674535320474 1103 80-81 0.40784918384375146 1103 82-83 0.2611170777656291 1103 84-85 0.3486977027162439 1103 86-87 0.34201210759516343 1103 88-89 0.2297129596121259 1103 90-91 0.15119760968363138 1103 92-93 0.11700098819976645 1103 94-95 0.10984089569034694 1103 96-97 0.18376190837012985 1103 98-99 0.08089920829128161 1103 100-101 0.17024627447846896 1103 102-103 -0.12989249647058188 1103 104-105 0.14474625329833302 1103 106-107 0.17758854936507618 1103 108-109 0.09348820256812829 1103 110-111 0.1900109545838511 1103 112-113 0.3118416012616443 1103 114-115 0.3447468005619747 1103 116-117 0.2550630019491962 1103 118-119 0.10400886225055928 1103 120-121 0.27846309130502434 1103 122-123 0.17996540970105102 1103 124-125 0.2084024114391383 1104 1 0.19588441256737354 1104 2 0.5053444845948469 1104 3 0.090190397949236 1104 4 0.4113497040212124 1104 5 0.12651449335960763 1104 6 0.15002717394754228 1104 7 0.1360232467952116 1104 8 0.09378956423215357 1104 9 0.24950671277991177 1104 10-11 0.17378960953942624 1104 12-13 0.15412182255982998 1104 14-15 0.2463480552781192 1104 16-17 0.3095822922907274 1104 18-19 0.19891904201784172 1104 20-21 0.07068688955358482 1104 22-23 -0.012222232102025998 1104 24-25 0.03259569855734412 1104 26-27 0.08907431974542135 1104 28-29 0.1774128167732556 1104 30-31 0.0705714227697527 1104 32-33 0.08605089458947646 1104 34-35 -0.006314830336336286 1104 36-37 -0.004297299049717651 1104 38-39 -0.02763132854477135 1104 40-41 -0.13684911495325025 1104 42-43 0.043991693839139145 1104 44-45 0.02878036168397813 1104 46-47 -0.06805783692178835 1104 48-49 -0.17022113131739758 1104 50-51 -0.10403371520587967 1104 52-53 -0.0818805862158456 1104 54-55 -0.14768676513515544 1104 56-57 0.02148783363248441 1104 58-59 -0.048345692047092825 1104 60-61 0.128213926957784 1104 62-63 -0.009317721441512106 1104 64-65 -0.0850903221142616 1104 66-67 0.03291302548388586 1104 68-69 0.12133566967444409 1104 70-71 0.031464784485514485 1104 72-73 -0.04550759339250732 1104 74-75 -0.008313979811070737 1104 76-77 0.02158736765619551 1104 78-79 -0.031975717182220365 1104 80-81 0.0013265260535852974 1104 82-83 -5.667778128604084E-4 1104 84-85 -0.10842150529108707 1104 86-87 -0.021739495141712695 1104 88-89 -0.008753952775439444 1104 90-91 0.11550434656910369 1104 92-93 -0.02131568565434705 1104 94-95 -0.040492093351360836 1104 96-97 -0.08079411907565515 1104 98-99 -0.19894009447486027 1104 100-101 -0.1763963527393706 1104 102-103 -0.28385258014018433 1104 104-105 -0.17809440770308171 1104 106-107 -0.23355749894997757 1104 108-109 -0.1832541601259301 1104 110-111 -0.2684649244667874 1104 112-113 -0.42923396994800456 1104 114-115 -0.3940230916647991 1104 116-117 -0.28415495871492524 1104 118-119 -0.47355710505168247 1104 120-121 -0.4882245039928428 1104 122-123 -0.5095262194882046 1104 124-125 -0.18979323412209936 1105 1 0.0828305265900795 1105 2 0.35297351661070664 1105 3 0.11618193034051671 1105 4 0.35040652578658893 1105 5 0.14973284165970568 1105 6 0.06201946468816999 1105 7 0.12797024305602633 1105 8 -0.005472312194441997 1105 9 0.04772477951465248 1105 10-11 0.05703759728569935 1105 12-13 0.05925318687536674 1105 14-15 0.16955990411130983 1105 16-17 0.20825595523071883 1105 18-19 0.06084220846425836 1105 20-21 0.03656363244311933 1105 22-23 0.010104257941826233 1105 24-25 0.08718453336482668 1105 26-27 0.08289572002623657 1105 28-29 0.038561678498382435 1105 30-31 0.05921765185883743 1105 32-33 0.0283043043459017 1105 34-35 0.1337479775084489 1105 36-37 0.09512694673055222 1105 38-39 0.04428761911410106 1105 40-41 0.03923575635261045 1105 42-43 0.08870743953682592 1105 44-45 0.21515363099560858 1105 46-47 0.1376153295246283 1105 48-49 0.25922433979774695 1105 50-51 0.21410380975958532 1105 52-53 0.24996248427309808 1105 54-55 0.2897894191287165 1105 56-57 0.24745494022937464 1105 58-59 0.32298410357400087 1105 60-61 0.3247439624725601 1105 62-63 0.33626773270463417 1105 64-65 0.31498436309566813 1105 66-67 0.25558171128698604 1105 68-69 0.28980818701887046 1105 70-71 0.28320356786039014 1105 72-73 0.19074310113536086 1105 74-75 0.18382727307966462 1105 76-77 0.11641963448253989 1105 78-79 0.021259257551982103 1105 80-81 -0.07328430370074202 1105 82-83 -0.010432123875450117 1105 84-85 0.010123960943595023 1105 86-87 0.09240815491385135 1105 88-89 0.023846035774237606 1105 90-91 0.012405790248525506 1105 92-93 0.052313654206297855 1105 94-95 0.18345525111193695 1105 96-97 0.15505205989479265 1105 98-99 0.047861792388427915 1105 100-101 0.1308787328564982 1105 102-103 0.0586900910890904 1105 104-105 0.09686342788527824 1105 106-107 0.1911850095345251 1105 108-109 0.07412871807265375 1105 110-111 0.016818733855533452 1105 112-113 0.16125397811632425 1105 114-115 0.05929349009426943 1105 116-117 0.10231366739632008 1105 118-119 -0.014177365368237105 1105 120-121 0.14987708384803966 1105 122-123 0.15128232358003046 1105 124-125 0.1536148986115613 1106 1 0.14315229603754176 1106 2 0.39100627055691817 1106 3 0.32176715341292805 1106 4 0.4292092344039631 1106 5 0.12252242149327941 1106 6 0.14311128660364858 1106 7 0.11744552289951571 1106 8 0.05912340225828672 1106 9 0.09853069231274958 1106 10-11 0.03200104570964157 1106 12-13 0.022088268920256837 1106 14-15 0.1894876811309203 1106 16-17 0.191228744143757 1106 18-19 0.0287550590159924 1106 20-21 0.023785917146668112 1106 22-23 0.1262242475753581 1106 24-25 0.037379815911066316 1106 26-27 0.08028232123420764 1106 28-29 0.14135317870665176 1106 30-31 0.10920007065076476 1106 32-33 0.2900713780104951 1106 34-35 0.2587338080771673 1106 36-37 0.2595060189139744 1106 38-39 0.049746795371163444 1106 40-41 0.049381624813165104 1106 42-43 0.0837920360425386 1106 44-45 0.1578916807751014 1106 46-47 0.23318775454444562 1106 48-49 0.11790387771647914 1106 50-51 0.027249574644379493 1106 52-53 0.06047440193169962 1106 54-55 0.2084751093589503 1106 56-57 0.2111281858092937 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124-125 -2.0647515917306904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64.0 3 21.0 4 64.0 5 235.0 6 731.0 7 1217.0 8 1961.0 9 4197.0 10 9149.0 11 12306.0 12 9364.0 13 10687.0 14 14500.0 15 14555.0 16 9275.0 17 8873.0 18 9100.0 19 8888.0 20 9068.0 21 8728.0 22 9101.0 23 9891.0 24 10812.0 25 12237.0 26 14469.0 27 17213.0 28 21427.0 29 25987.0 30 32147.0 31 39954.0 32 51581.0 33 67507.0 34 96493.0 35 150117.0 36 242763.0 37 336537.0 38 121924.0 39 689.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.62864172549381 16.871867023845112 10.225143139644507 23.27434811101658 2 11.955259967140899 23.55803792428058 35.42777813650158 29.05892397207694 3 23.553859829491525 29.97823982964936 28.147745314891115 18.320155025967995 4 13.901818870566899 19.038736375689467 32.85840761296914 34.201037140774496 5 10.88237319849164 39.265707775399044 34.41089026511086 15.441028760998456 6 31.411081503420192 27.17605766181512 21.041741104419486 20.371119730345207 7 22.243211520470187 32.03650081214953 24.46428263951466 21.256005027865626 8 28.54260771742936 31.406510971193086 24.10591807334026 15.944963238037296 9 29.877847545471763 14.339676517686492 22.2877649530216 33.49471098382015 10-11 23.692004267375314 27.43575081914522 26.30268662413162 22.56955828934785 12-13 27.689078448950983 25.368330913608467 25.792114602761533 21.150476034679016 14-15 28.69161879104106 21.950814408784602 26.423901521198307 22.93366527897604 16-17 22.700299822861293 25.7914383677124 33.51089055066533 17.997371258760978 18-19 25.428674330909317 23.85570857893921 32.736047098933014 17.97956999121845 20-21 20.483000425446736 24.76630921009679 34.52181006421878 20.22888030023769 22-23 19.560929868162017 27.231007754162622 27.342032612251693 25.866029765423665 24-25 20.78704607154951 29.026561307245064 26.215533866348313 23.97085875485711 26-27 23.415949698385457 22.969626181634514 27.540370718996265 26.074053400983765 28-29 22.321186432165852 22.86494153502661 31.41116609964228 23.402705933165258 30-31 25.82269599205643 24.738060254033485 29.986971170126676 19.45227258378341 32-33 18.86149119836537 24.98123877196104 32.443580001033126 23.713690028640467 34-35 16.565076085318868 26.990271410430257 33.654032414282945 22.79062008996793 36-37 23.965872501133564 24.65074700537798 28.982510087298902 22.400870406189554 38-39 26.025518139919303 23.17158739360267 29.021395691876783 21.781498774601243 40-41 21.892941788833003 26.232027495452282 29.153293746979998 22.72173696873472 42-43 25.376515964621277 25.055602109866896 27.87778584506598 21.69009608044585 44-45 27.516013407641665 21.817622209850253 28.313311790804057 22.353052591704024 46-47 22.76138731210074 24.354549185267665 34.6591626537488 18.22490084888279 48-49 25.675081358442046 23.920135281726925 31.64172583209454 18.763057527736485 50-51 20.45278771042708 25.394452129094468 32.88448679611316 21.26827336436529 52-53 19.71675926510512 27.856764660303394 25.87320423121294 26.55327184337854 54-55 20.944166872334684 29.10422489941399 24.924775726199428 25.0268325020519 56-57 23.627524694511244 23.494402481791205 25.95890322506586 26.919169598631687 58-59 22.794174620757737 23.29671007696767 29.60963014193963 24.29948516033496 60-61 25.87747303835756 25.22057895069133 28.271448782923624 20.63049922802748 62-63 19.164146037686034 25.53489229691957 30.617140372727846 24.683821292666547 64-65 16.9386984945101 26.969354986827682 32.198536122000355 23.893410396661867 66-67 23.782493155559635 25.08846116318179 27.63952183620408 23.489523845054496 68-69 25.684157057665484 23.681943017522915 27.826954754948947 22.80694516986265 70-71 21.838141182007586 26.30611149693794 27.988416107536633 23.867331213517843 72-73 25.00408944549989 25.414002548370245 26.802261678595414 22.779646327534454 74-75 26.936962273789096 22.1353434273284 27.34780805721206 23.579886241670444 76-77 22.49643429050273 24.458471322225346 33.62356438939557 19.42152999787636 78-79 24.93108925609399 24.170560010101646 31.019735520493143 19.878615213311218 80-81 20.017979211267928 25.54095472051151 32.094434623397945 22.346631444822616 82-83 19.46382347370415 27.750726055937875 25.54321467723513 27.242235793122845 84-85 20.56639537620029 28.732408209884692 24.77009424378261 25.931102170132412 86-87 22.945412359595704 23.436576287529633 25.833959903345598 27.784051449529066 88-89 22.14897491232803 23.136037915616804 29.275945738080345 25.439041433974825 90-91 24.856797662846024 25.005990678934047 27.91620510936756 22.221006548852372 92-93 18.934419643113372 25.286978631571095 29.861238657169586 25.917363068145942 94-95 16.829965888500706 26.629425858518168 31.481157961460354 25.05945029152077 96-97 23.148421516720052 24.846309398722404 27.358738633962567 24.646530450594977 98-99 24.75685576868231 23.63891785705189 27.37242080211857 24.231805572147227 100-101 21.335533981139765 25.75371350349253 27.749649886069484 25.16110262929822 102-103 24.000318557822727 24.85206612370734 26.894854035585926 24.252761282884013 104-105 25.75904249059225 21.868568500759224 27.474431875263715 24.89795713338481 106-107 21.772667274444863 24.13475023868031 33.02589228405128 21.066690202823548 108-109 23.946866864338425 24.024187043793003 30.26839251526311 21.760553576605464 110-111 19.392431658248547 25.360266528704717 31.64927910816499 23.598022704881743 112-113 18.826220250535027 27.18145402163793 25.741209478210486 28.251116249616555 114-115 19.61324231998625 28.362516960931274 25.000367838234432 27.023872880848042 116-117 21.86305994774234 23.493375564357276 25.873930632456954 28.769633855443434 118-119 20.99579199359327 23.442736237508377 28.750536406000858 26.8109353628975 120-121 23.39279897017438 24.91303312060412 27.696619054931208 23.997548854290294 122-123 18.37108438921494 25.29312373545323 29.144592403392217 27.19119947193962 124-125 16.43666079544843 26.76371515384146 30.464161055527857 26.33546299518225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 920.0 1 792.5 2 2343.5 3 3249.0 4 1646.5 5 881.5 6 1226.0 7 2267.5 8 3265.0 9 3326.5 10 2668.0 11 1956.0 12 1775.5 13 1898.0 14 2232.0 15 2672.0 16 2876.5 17 3106.5 18 3396.0 19 3526.5 20 3619.0 21 3942.0 22 4406.5 23 4839.5 24 5259.5 25 5769.5 26 6499.5 27 7227.5 28 7863.0 29 8487.5 30 9488.0 31 10867.0 32 12216.5 33 13457.5 34 14728.5 35 15676.5 36 16941.0 37 18065.0 38 18186.0 39 18212.0 40 18059.5 41 19271.5 42 21362.5 43 22393.0 44 23264.0 45 24933.5 46 28599.0 47 39028.5 48 53743.5 49 98994.5 50 254062.5 51 260739.5 52 95129.0 53 32773.5 54 23609.0 55 19700.0 56 16543.5 57 14006.0 58 12685.5 59 11588.5 60 10331.5 61 8607.0 62 7099.0 63 5781.0 64 4553.0 65 3594.0 66 2551.5 67 2041.0 68 1640.5 69 1079.5 70 878.5 71 839.5 72 800.5 73 721.0 74 551.5 75 434.0 76 256.0 77 112.0 78 64.0 79 45.0 80 28.5 81 10.0 82 5.0 83 1.5 84 1.0 85 1.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1566903328378169 2 1.4348931578554662E-4 3 7.174465789277331E-5 4 0.0 5 0.0 6 0.0035872328946386653 7 0.0 8 0.0 9 0.0 10-11 7.174465789277331E-5 12-13 0.005631955644582704 14-15 0.0021164674078368124 16-17 6.457019210349597E-4 18-19 0.0 20-21 2.1523397367831991E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.3046794735663983E-4 30-31 0.0 32-33 0.0 34-35 1.4348931578554662E-4 36-37 0.0 38-39 0.0 40-41 1.0761698683915996E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0014707654868018527 96-97 0.002977403302550092 98-99 0.004340551802512785 100-101 0.0 102-103 0.0036231052235850517 104-105 0.02152339736783199 106-107 0.01660888830217702 108-109 0.01980152557840543 110-111 0.015138122815375167 112-113 0.015389229117999875 114-115 0.03992590211732835 116-117 0.03899322156472229 118-119 0.021379908052046447 120-121 0.014205442262769114 122-123 0.0037307222104242116 124-125 0.0019371057631048794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1393832.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.081609168485485 #Duplication Level Percentage of deduplicated Percentage of total 1 88.37565656288581 41.60868122302089 2 7.945818842867151 7.482038745669177 3 1.7091616566471635 2.414102433720688 4 0.6615062555878182 1.245791159523757 5 0.3374767006170323 0.794447306096055 6 0.19302109784156865 0.545264633390924 7 0.13323774646523565 0.4391133253896189 8 0.09627552718380615 0.36262453946862894 9 0.06396052530713535 0.27102280092494146 >10 0.4106336686917873 3.6132275859993763 >50 0.03368987984414079 1.070154828603605 >100 0.028384299862271662 2.7876992986753937 >500 0.004706204715433733 1.486495917525802 >1k 0.002794309049788779 3.17801859170308 >5k 0.0019118956656449541 6.999991535604313 >10k+ 0.00176482676828765 25.701326074683696 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 79216 5.68332481963393 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 66540 4.773889536185136 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 41636 2.987160576023509 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 36894 2.6469474082959783 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 24702 1.772236539267286 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 23353 1.6754529957699351 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 21183 1.519767088142617 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 20609 1.4785856545121652 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 18681 1.3402619540948981 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 14911 1.0697845938391428 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12865 0.9229950237905287 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10589 0.7597041824265766 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9960 0.7145767926120221 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 9869 0.7080480287437798 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9393 0.6738975715868196 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8850 0.6349402223510437 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8587 0.6160713773252444 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 8001 0.5740290078000791 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7692 0.5518599085112122 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7295 0.5233772793277812 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6753 0.4844916747498981 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6577 0.47186461496077003 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6555 0.470286232487129 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5832 0.4184148448306539 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5730 0.41109688972559105 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4931 0.35377290806926515 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4425 0.3174701111755219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4045 0.29020714117626806 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3789 0.271840508755718 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 3740 0.26832502051897217 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 3478 0.24952792015106554 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 3068 0.2201126104150285 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2523 0.18101177186346704 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2446 0.1754874332057235 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2161 0.1550402057062831 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1541 0.11055851781276366 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 1433 0.10281009476034414 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.022126052494131E-4 0.0 12-13 0.0 0.0 0.0 0.0014348931578554661 0.0 14-15 0.0 0.0 0.0 0.0037307222104242116 0.0 16-17 0.0 0.0 0.0 0.005847189618261024 0.0 18-19 3.5872328946386655E-5 0.0 0.0 0.006923359486652623 0.0 20-21 7.174465789277331E-5 0.0 0.0 0.00900395456554305 0.0 22-23 7.174465789277331E-5 0.0 0.0 0.013954335960144407 0.0 24-25 7.174465789277331E-5 0.0 0.0 0.019550419275780724 0.0 26-27 7.174465789277331E-5 0.0 0.0 0.024464928341435696 0.0 28-29 7.174465789277331E-5 0.0 0.0 0.03239271303858715 0.0 30-31 7.174465789277331E-5 0.0 0.0 0.04401534761721643 0.0 32-33 7.174465789277331E-5 0.0 0.0 0.06295593730090858 0.0 34-35 7.174465789277331E-5 0.0 0.0 0.09764447939206447 0.0 36-37 7.174465789277331E-5 0.0 0.0 0.1410858697461387 0.0 38-39 7.174465789277331E-5 0.0 0.0 0.211252145165271 0.0 40-41 7.174465789277331E-5 0.0 0.0 0.32981019233307884 0.0 42-43 1.7936164473193327E-4 0.0 0.0 0.4123524212387145 0.0 44-45 2.1523397367831991E-4 0.0 0.0 0.4967958835785088 0.0 46-47 2.1523397367831991E-4 0.0 0.0 0.6098654644175194 0.0 48-49 2.1523397367831991E-4 0.0 0.0 0.7075816884674767 0.0 50-51 2.1523397367831991E-4 0.0 0.0 0.8111809744646414 0.0 52-53 2.1523397367831991E-4 7.174465789277331E-5 0.0 0.9472805904872323 0.0 54-55 2.1523397367831991E-4 7.174465789277331E-5 0.0 1.0807973988256836 0.0 56-57 2.1523397367831991E-4 7.174465789277331E-5 0.0 1.234761434663575 0.0 58-59 2.1523397367831991E-4 7.174465789277331E-5 0.0 1.4167417594085945 0.0 60-61 2.1523397367831991E-4 7.174465789277331E-5 0.0 1.5780954950094417 0.0 62-63 2.1523397367831991E-4 7.174465789277331E-5 0.0 1.7329204667420464 0.0 64-65 2.1523397367831991E-4 7.174465789277331E-5 0.0 1.9234025334473595 0.0 66-67 2.1523397367831991E-4 7.174465789277331E-5 0.0 2.1291662122838333 0.0 68-69 2.1523397367831991E-4 7.174465789277331E-5 0.0 2.3859762152110155 0.0 70-71 2.1523397367831991E-4 7.174465789277331E-5 0.0 2.808695739515236 0.0 72-73 2.1523397367831991E-4 7.174465789277331E-5 0.0 3.1205697673751214 0.0 74-75 2.1523397367831991E-4 7.174465789277331E-5 0.0 3.4233322236826247 0.0 76-77 2.1523397367831991E-4 7.174465789277331E-5 0.0 3.7773562380545145 0.0 78-79 2.1523397367831991E-4 7.174465789277331E-5 0.0 4.092853371137985 0.0 80-81 2.1523397367831991E-4 7.174465789277331E-5 0.0 4.421264542642155 0.0 82-83 2.1523397367831991E-4 7.174465789277331E-5 0.0 4.854494659327666 0.0 84-85 2.5110630262470656E-4 7.174465789277331E-5 0.0 5.266237250974292 0.0 86-87 2.8697863157109324E-4 7.174465789277331E-5 0.0 5.688705668975889 0.0 88-89 2.8697863157109324E-4 7.174465789277331E-5 0.0 6.174703981541534 0.0 90-91 2.8697863157109324E-4 7.174465789277331E-5 0.0 6.605781758490263 0.0 92-93 2.8697863157109324E-4 7.174465789277331E-5 0.0 7.043998128899322 0.0 94-95 2.8697863157109324E-4 7.174465789277331E-5 0.0 7.5434485648198635 0.0 96-97 2.8697863157109324E-4 7.174465789277331E-5 0.0 8.037159428108982 0.0 98-99 3.5872328946386653E-4 7.174465789277331E-5 0.0 8.630021408605915 0.0 100-101 3.5872328946386653E-4 7.174465789277331E-5 0.0 9.537160863002141 0.0 102-103 3.5872328946386653E-4 7.174465789277331E-5 0.0 10.238393149246107 0.0 104-105 3.5872328946386653E-4 7.174465789277331E-5 0.0 10.897547193635962 0.0 106-107 3.5872328946386653E-4 7.174465789277331E-5 0.0 11.585793696801336 0.0 108-109 3.5872328946386653E-4 7.174465789277331E-5 0.0 12.169723467390618 0.0 110-111 3.5872328946386653E-4 7.174465789277331E-5 0.0 12.784933908821149 0.0 112-113 3.5872328946386653E-4 7.174465789277331E-5 0.0 13.556009619523731 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 305 0.0 68.27672 3 TATAACG 280 0.0 65.8731 2 TATCACG 90 2.5465852E-11 59.49828 2 TAACGCA 365 0.0 58.683235 4 AGTACTC 1575 0.0 57.798332 5 AAAGTAC 1690 0.0 53.161182 3 GTATAAC 400 0.0 52.13019 1 AAGTACT 1740 0.0 51.63356 4 AAAAGTA 1780 0.0 49.470478 2 ATCACGC 125 1.2732926E-11 47.59862 3 AAAAAGT 1935 0.0 46.184 1 GTTATCA 680 0.0 36.797783 1 TCACGCA 175 4.456524E-10 33.999016 4 CTACGTA 235 0.0 32.91394 2 AACCGAT 110 3.6379788E-12 32.458263 98-99 GCTACGT 240 0.0 32.27107 1 TTATCAA 770 0.0 31.6809 2 GTGTCGA 115 7.2759576E-12 31.041468 62-63 GTTCAAC 280 0.0 29.78868 1 TACGTAA 260 1.8189894E-12 29.749138 3 >>END_MODULE