FastQCFastQC Report
Thu 26 May 2016
SRR1364135_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364135_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497360
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA739104.9360207298178125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT523013.4928808035475774No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG354192.3654298231554205No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC185661.2399155847625154No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT180631.2063231287065235No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG179391.1980418870545493No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC158731.0600657156595608No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG139060.9287011807447775No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT120450.8044157717582946No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG113420.7574664743281508No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT82270.549433669925736No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC81680.5454934017203612No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG74700.49887802532457126No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG70580.47136293209381847No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA69980.46735587968157294No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG61680.4119249879788428No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC59050.39436074157183315No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA58140.388283378746594No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54610.364708553721216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53050.3542902174493776No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC50950.34026553400651816No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA46620.3113479724314794No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC46390.30981193567345194No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC37240.24870438638670728No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT36070.24089063418282847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28950.1933402788908479No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG28320.18913287385799007No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC21180.141448950152268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC17000.054.9880265
AAGTACT20300.046.3408364
TATAACG1401.882654E-938.2713282
AAAGTAC23850.037.94533
AAAAGTA25650.035.2789232
AAAAAGT26550.033.862211
ATAACGC1659.365976E-932.4759143
GTACAAA13900.030.4120961
CATGGGG57850.026.0387384
ATGGGAG24650.024.1546345
GTTAGTC1501.6794096E-423.8156723
ATGGGGG21400.023.3713025
ATGGGGA33600.021.6191185
GAACAAA17250.021.3995881
GTCGGGA3451.2751116E-920.7071932
CATGGGT34950.019.7612134
TGGGGGG13350.019.618136
GTACACG2801.5569658E-619.137591
ATGGGAT27700.019.1305585
TGGGTAC5101.8189894E-1218.6739436
Produced by FastQC (version 0.11.5)