##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364135_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1497360 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09861890260191 33.0 31.0 34.0 30.0 34.0 2 31.908911684564835 33.0 31.0 34.0 30.0 34.0 3 32.142883474915855 34.0 31.0 34.0 30.0 34.0 4 35.600928968317575 37.0 35.0 37.0 33.0 37.0 5 35.739113506437995 37.0 35.0 37.0 33.0 37.0 6 35.8859205535075 37.0 35.0 37.0 35.0 37.0 7 35.85762141368809 37.0 35.0 37.0 35.0 37.0 8 35.9137228188278 37.0 35.0 37.0 35.0 37.0 9 37.58713602607256 39.0 37.0 39.0 35.0 39.0 10-11 37.54197354009724 39.0 37.0 39.0 35.0 39.0 12-13 37.52421394988514 39.0 37.0 39.0 34.5 39.0 14-15 38.8200359299033 40.0 38.0 41.0 34.0 41.0 16-17 38.7956803974996 40.0 38.0 41.0 34.0 41.0 18-19 38.856129120585564 40.0 38.0 41.0 35.0 41.0 20-21 38.89704145963562 40.0 38.5 41.0 35.0 41.0 22-23 38.850629775070786 40.0 38.0 41.0 35.0 41.0 24-25 38.81448382486509 40.0 38.0 41.0 35.0 41.0 26-27 38.62149416306032 40.0 38.0 41.0 34.0 41.0 28-29 38.46324531174868 40.0 38.0 41.0 34.0 41.0 30-31 38.303644414168936 40.0 38.0 41.0 34.0 41.0 32-33 37.96183750066784 40.0 37.0 41.0 33.5 41.0 34-35 37.72297610461078 40.0 37.0 41.0 33.0 41.0 36-37 37.59313324784955 40.0 37.0 41.0 33.0 41.0 38-39 37.489756972271195 40.0 37.0 41.0 33.0 41.0 40-41 37.23708026125982 40.0 36.0 41.0 32.0 41.0 42-43 37.05345073996901 40.0 36.0 41.0 31.5 41.0 44-45 36.80521016989903 40.0 36.0 41.0 31.0 41.0 46-47 36.441499372228456 40.0 35.5 41.0 30.0 41.0 48-49 36.06507553293797 39.5 35.0 41.0 29.5 41.0 50-51 35.895093698242235 39.0 35.0 41.0 28.0 41.0 52-53 35.78157958006091 39.0 35.0 41.0 28.0 41.0 54-55 35.58738680076935 39.0 35.0 41.0 28.0 41.0 56-57 35.29551844579794 39.0 35.0 41.0 26.5 41.0 58-59 35.10671415023775 39.0 35.0 41.0 26.0 41.0 60-61 34.9326457899236 38.5 35.0 40.0 26.0 41.0 62-63 34.5756200913608 38.0 34.0 40.0 26.0 41.0 64-65 34.279793102527115 37.5 34.0 40.0 25.5 41.0 66-67 33.82935666773521 37.0 34.0 39.5 24.0 41.0 68-69 33.51263323449271 36.5 34.0 39.0 23.0 41.0 70-71 33.024455040871935 36.0 33.0 39.0 22.0 40.5 72-73 32.683148675001334 35.0 33.0 38.5 22.0 39.5 74-75 32.29285075065449 35.0 33.0 37.0 20.5 39.0 76-77 31.745870398568147 35.0 32.0 37.0 20.0 39.0 78-79 31.39387355078271 35.0 32.0 36.0 19.0 38.0 80-81 31.219944769460916 35.0 32.0 36.0 18.0 37.0 82-83 31.010615015761072 35.0 32.0 36.0 18.0 37.0 84-85 30.790477239942298 35.0 32.0 35.0 16.0 36.0 86-87 30.518474515146657 35.0 32.0 35.0 11.5 36.0 88-89 30.274538187209487 35.0 31.0 35.0 7.5 36.0 90-91 30.1247268525939 35.0 31.0 35.0 7.0 35.5 92-93 29.93559631618315 34.0 31.0 35.0 4.5 35.0 94-95 29.84961732649463 34.0 31.0 35.0 2.0 35.0 96-97 29.762350403376608 34.5 31.0 35.0 2.0 35.0 98-99 29.650101511994443 35.0 31.0 35.0 2.0 35.0 100-101 29.392746567291766 34.0 30.5 35.0 2.0 35.0 102-103 28.00597551690976 33.0 28.0 34.5 2.0 35.0 104-105 28.82203311160977 34.0 29.0 35.0 2.0 35.0 106-107 28.833315635518513 34.0 29.5 35.0 2.0 35.0 108-109 28.827440962761127 34.0 29.0 35.0 2.0 35.0 110-111 28.891636613773574 34.0 30.0 35.0 2.0 35.0 112-113 28.819894347384732 34.0 30.0 35.0 2.0 35.0 114-115 28.72301483945077 34.0 30.0 35.0 2.0 35.0 116-117 28.47472251162045 34.0 29.0 35.0 2.0 35.0 118-119 28.245028917561577 34.0 29.0 35.0 2.0 35.0 120-121 28.059103689159585 34.0 29.0 35.0 2.0 35.0 122-123 27.73799787626222 34.0 29.0 35.0 2.0 35.0 124-125 26.688643345621628 33.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32137317568365376 1101 2 0.6224759765278414 1101 3 0.45133702619271077 1101 4 0.5270344235928377 1101 5 0.2381186690361261 1101 6 0.22809993613402924 1101 7 0.2214038709286541 1101 8 0.23732737272018767 1101 9 0.26510801000932105 1101 10-11 0.2438549347231529 1101 12-13 0.20397952625225457 1101 14-15 0.3599798075149181 1101 16-17 0.3982667911481599 1101 18-19 0.30807147868867446 1101 20-21 0.28509463266633617 1101 22-23 0.31147162786142957 1101 24-25 0.2831936319287891 1101 26-27 0.2929465629302612 1101 28-29 0.41187663865505897 1101 30-31 0.42001750984939434 1101 32-33 0.4047229271564845 1101 34-35 0.43282656184172197 1101 36-37 0.5163130458342451 1101 38-39 0.4621775551552574 1101 40-41 0.4525982413517795 1101 42-43 0.4054035037617183 1101 44-45 0.39732846744082906 1101 46-47 0.39566223084932517 1101 48-49 0.4474009950113427 1101 50-51 0.5486450720777185 1101 52-53 0.4707878995457264 1101 54-55 0.6057087337757281 1101 56-57 0.6542567079193162 1101 58-59 0.5460710037976213 1101 60-61 0.33993622832979753 1101 62-63 0.4544897890322446 1101 64-65 0.3498227960752942 1101 66-67 0.465982075412569 1101 68-69 0.43060810044008946 1101 70-71 0.3084260854786791 1101 72-73 0.3805282902953664 1101 74-75 0.27281121804960407 1101 76-77 0.3574745386510578 1101 78-79 0.42329041746866736 1101 80-81 0.3713321236050078 1101 82-83 0.3289154297009702 1101 84-85 0.4045893630476485 1101 86-87 0.4158584498601776 1101 88-89 0.35272159291821836 1101 90-91 0.35117036897246834 1101 92-93 0.2801393124023761 1101 94-95 0.27083953499244373 1101 96-97 0.33367722127026767 1101 98-99 0.3544597109653651 1101 100-101 0.17541873022211263 1101 102-103 0.15292709336891264 1101 104-105 0.3236495527898917 1101 106-107 0.31591157988666296 1101 108-109 0.3695357312994929 1101 110-111 0.24080303819615523 1101 112-113 0.2977569362600576 1101 114-115 0.27372676943463503 1101 116-117 0.3251839369347067 1101 118-119 0.3124825250884271 1101 120-121 0.30161663632969393 1101 122-123 0.3772630410363007 1101 124-125 0.39565801257671396 1102 1 0.3055145440570115 1102 2 0.5690097327580155 1102 3 0.466289450297829 1102 4 0.546970989066331 1102 5 0.3160525158870513 1102 6 0.2394275301530584 1102 7 0.18787419263232152 1102 8 0.17525215749590473 1102 9 0.2709983588992131 1102 10-11 0.23298044446488575 1102 12-13 0.2336578225821171 1102 14-15 0.3534098029838759 1102 16-17 0.3672291835360184 1102 18-19 0.26683903645939466 1102 20-21 0.19198180982990465 1102 22-23 0.29697230751706627 1102 24-25 0.30131778235924145 1102 26-27 0.2725568936960059 1102 28-29 0.348668664028871 1102 30-31 0.3407243517161831 1102 32-33 0.4321357046825369 1102 34-35 0.40767930311947254 1102 36-37 0.4800647449733475 1102 38-39 0.4775830830211376 1102 40-41 0.5284931212792898 1102 42-43 0.4341755925700497 1102 44-45 0.5334861475495813 1102 46-47 0.6056758239621445 1102 48-49 0.4184023543226303 1102 50-51 0.4677660507818402 1102 52-53 0.5274258696408793 1102 54-55 0.5024010763221796 1102 56-57 0.40074515377341413 1102 58-59 0.41580367257877526 1102 60-61 0.43055698048203794 1102 62-63 0.41484320996563895 1102 64-65 0.3840321300127627 1102 66-67 0.27069435452447976 1102 68-69 0.3676266776942825 1102 70-71 0.3322140329186425 1102 72-73 0.24346440266509717 1102 74-75 0.23407084650452248 1102 76-77 0.2888293188957327 1102 78-79 0.20851923486785395 1102 80-81 0.07477571218679202 1102 82-83 0.16693083522883967 1102 84-85 0.23263648583876773 1102 86-87 0.1700496596472192 1102 88-89 0.1630976690396544 1102 90-91 0.2100285474953516 1102 92-93 0.21987651222113413 1102 94-95 0.26562884174369117 1102 96-97 0.2115657577451202 1102 98-99 0.1650623389671786 1102 100-101 0.08253245926606567 1102 102-103 0.06638427506351974 1102 104-105 0.22736500362813317 1102 106-107 0.22008013448566643 1102 108-109 0.27982118666877653 1102 110-111 0.11937123031214725 1102 112-113 0.2653600173656301 1102 114-115 0.21504983241605657 1102 116-117 0.14620795143402532 1102 118-119 0.15253689753971855 1102 120-121 0.11516443877644988 1102 122-123 -0.015577919543673602 1102 124-125 0.07123571987168731 1103 1 0.14244038247547763 1103 2 0.4325605046748855 1103 3 0.33354894502276267 1103 4 0.46723739414274945 1103 5 0.16328158496440182 1103 6 0.19609797752968916 1103 7 0.14858394997885682 1103 8 0.08886508062982301 1103 9 0.2658524540892131 1103 10-11 0.15671863770637628 1103 12-13 0.1137697238445341 1103 14-15 0.273370331873501 1103 16-17 0.29533260166606823 1103 18-19 0.17376109970538067 1103 20-21 0.13936767938835004 1103 22-23 0.1259775516428121 1103 24-25 0.06217088412650895 1103 26-27 -0.01337224104193524 1103 28-29 0.05972977406248248 1103 30-31 0.03758412669264288 1103 32-33 -0.01021296759054735 1103 34-35 0.08512974930831518 1103 36-37 0.09651147704034457 1103 38-39 0.030026001707078365 1103 40-41 -0.05538911410459235 1103 42-43 -0.25814369648481517 1103 44-45 -0.3219492494478118 1103 46-47 -0.36198166281955935 1103 48-49 -0.28286075028121616 1103 50-51 -0.19113413639860255 1103 52-53 -0.30726019922507675 1103 54-55 -0.26126461201138085 1103 56-57 -0.3024629537521406 1103 58-59 -0.5964115438166857 1103 60-61 -0.5799438341495176 1103 62-63 -0.49790161038556846 1103 64-65 -0.44489866970499037 1103 66-67 -0.4982339408709322 1103 68-69 -0.5081368187550197 1103 70-71 -0.556742528509723 1103 72-73 -0.5564916367179649 1103 74-75 -0.4590126138792172 1103 76-77 -0.48401307035237195 1103 78-79 -0.4946774516188164 1103 80-81 -0.40740725950107404 1103 82-83 -0.4824554189071364 1103 84-85 -0.5571283528940896 1103 86-87 -0.6081987226145387 1103 88-89 -0.5278627240434339 1103 90-91 -0.5461073537305232 1103 92-93 -0.4322639773551984 1103 94-95 -0.47730714187978407 1103 96-97 -0.39604534715940787 1103 98-99 -0.4167616175547977 1103 100-101 -0.38876252457212956 1103 102-103 -0.5559192695701078 1103 104-105 -0.44582674481663176 1103 106-107 -0.37476391019011857 1103 108-109 -0.29629204275664023 1103 110-111 -0.3551117807886861 1103 112-113 -0.2438076505454454 1103 114-115 -0.24913233606129737 1103 116-117 -0.1587206006232762 1103 118-119 -0.21642225542619897 1103 120-121 -0.19722360908559367 1103 122-123 -0.07939162454114523 1103 124-125 -0.05413447752954781 1104 1 0.234481459420131 1104 2 0.5186898189283546 1104 3 0.12278822401523115 1104 4 0.45284513264378745 1104 5 0.21431217820381931 1104 6 0.10345508240914825 1104 7 0.14513368886478872 1104 8 0.12894184914348017 1104 9 0.2746880908585112 1104 10-11 0.2144579134369522 1104 12-13 0.1340925038856966 1104 14-15 0.21775128277883482 1104 16-17 0.25954256444793344 1104 18-19 0.19851943464247057 1104 20-21 -0.009718095195680121 1104 22-23 0.09514904862067652 1104 24-25 0.17274233413319706 1104 26-27 0.16384323269824108 1104 28-29 0.23812686641628744 1104 30-31 0.2397640458630761 1104 32-33 0.26300575662016 1104 34-35 0.2760588081560229 1104 36-37 0.22825767123353558 1104 38-39 0.24261602979192531 1104 40-41 0.32761718786134963 1104 42-43 0.2814935327377768 1104 44-45 0.3543039675883719 1104 46-47 0.3420116617284279 1104 48-49 0.30079381097866076 1104 50-51 0.3753021458981536 1104 52-53 0.3952583446032705 1104 54-55 0.40919039677940106 1104 56-57 0.5059480668597303 1104 58-59 0.545239574521311 1104 60-61 0.47791327725371957 1104 62-63 0.3749673579773898 1104 64-65 0.5031835037556434 1104 66-67 0.4830762613334514 1104 68-69 0.5454835089994035 1104 70-71 0.4158943158101849 1104 72-73 0.38729300044602155 1104 74-75 0.48669775990043007 1104 76-77 0.46096500976610244 1104 78-79 0.4798961381858291 1104 80-81 0.4481129544134852 1104 82-83 0.47583462646571206 1104 84-85 0.28608741115404257 1104 86-87 0.30789942430738293 1104 88-89 0.3498360798225555 1104 90-91 0.23793655453216545 1104 92-93 0.23978385169581884 1104 94-95 0.37865444613734667 1104 96-97 0.37735053829616305 1104 98-99 0.2350380471232043 1104 100-101 0.2913668337168147 1104 102-103 0.11673160086447254 1104 104-105 0.29056038084104685 1104 106-107 0.24927831820069812 1104 108-109 0.22639132422849428 1104 110-111 0.1899085815417365 1104 112-113 0.2649535744236502 1104 114-115 0.3213961875107323 1104 116-117 0.2644022241057442 1104 118-119 0.22329888584286905 1104 120-121 0.3174306227236805 1104 122-123 0.18737406758237896 1104 124-125 0.30379565937185404 1105 1 0.17095077871041298 1105 2 0.4990002701157259 1105 3 0.17085009766253023 1105 4 0.47230307225464685 1105 5 0.15527475553204084 1105 6 0.18297233136611624 1105 7 0.23041844170694503 1105 8 0.16130014462622455 1105 9 0.17401120703779327 1105 10-11 0.1696795745096935 1105 12-13 0.242475531753108 1105 14-15 0.3234041084901449 1105 16-17 0.30119892200195153 1105 18-19 0.20997944608854624 1105 20-21 0.2174172151685596 1105 22-23 0.14827154380272844 1105 24-25 0.1769377806804826 1105 26-27 0.21939801973634587 1105 28-29 0.29823676616823747 1105 30-31 0.3280612992783247 1105 32-33 0.29775118806936973 1105 34-35 0.23491764274278637 1105 36-37 0.34970814164525876 1105 38-39 0.28111147507444656 1105 40-41 0.3272379574362816 1105 42-43 0.36247750822672486 1105 44-45 0.39332873475393626 1105 46-47 0.28891633912763837 1105 48-49 0.34621875853063955 1105 50-51 0.4173029529732233 1105 52-53 0.4231529260546125 1105 54-55 0.51729250956123 1105 56-57 0.4983747226283768 1105 58-59 0.5805083992903803 1105 60-61 0.4908619509155585 1105 62-63 0.5427029116498474 1105 64-65 0.46320562124869724 1105 66-67 0.5219119073334113 1105 68-69 0.5066724665366564 1105 70-71 0.5458424922631977 1105 72-73 0.513738581769303 1105 74-75 0.5111220568914021 1105 76-77 0.4977151848563359 1105 78-79 0.5364269643429687 1105 80-81 0.5049209408995239 1105 82-83 0.5744168501037663 1105 84-85 0.513378247492497 1105 86-87 0.5108683301791892 1105 88-89 0.5013069168250119 1105 90-91 0.5228156750811941 1105 92-93 0.5358019519944648 1105 94-95 0.6106363569053528 1105 96-97 0.6865373607602052 1105 98-99 0.5945293210686735 1105 100-101 0.6257948103693458 1105 102-103 0.5483321864484232 1105 104-105 0.6966283245465696 1105 106-107 0.5669888335746478 1105 108-109 0.69496075007482 1105 110-111 0.8119196964119979 1105 112-113 0.6459583196456222 1105 114-115 0.7484163651779134 1105 116-117 0.7440325702864037 1105 118-119 0.7277306844424665 1105 120-121 0.6529476764332998 1105 122-123 0.6410125058565512 1105 124-125 0.6553768466970631 1106 1 0.2832092484265445 1106 2 0.5138384295400336 1106 3 0.4193894615070164 1106 4 0.5211365755988879 1106 5 0.2838714922514569 1106 6 0.15480231169232184 1106 7 0.08355514285563714 1106 8 0.07511622636767612 1106 9 0.2346277042353222 1106 10-11 0.18931128678003972 1106 12-13 0.14499108039815667 1106 14-15 0.2589935174558846 1106 16-17 0.18454054949387455 1106 18-19 0.18163954450396602 1106 20-21 0.105702348218216 1106 22-23 0.12183014029178452 1106 24-25 0.17605383363100913 1106 26-27 0.16443609418099925 1106 28-29 0.3060354681001698 1106 30-31 0.1873310663350125 1106 32-33 0.24575464994538976 1106 34-35 0.3075308405936852 1106 36-37 0.3520207994342144 1106 38-39 0.3066194955129049 1106 40-41 0.383732883423491 1106 42-43 0.3565017475504817 1106 44-45 0.3628039737882318 1106 46-47 0.255216740652628 1106 48-49 0.21543880707274354 1106 50-51 0.17765452726386144 1106 52-53 0.20891306827557088 1106 54-55 0.20183224954515566 1106 56-57 0.2906773500320696 1106 58-59 0.25287981532382275 1106 60-61 0.2899165135799251 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-1.1003350982078004 2216 108-109 -1.2723343799333229 2216 110-111 -1.3162808668120505 2216 112-113 -1.3875675526703475 2216 114-115 -1.434920506476555 2216 116-117 -1.5953202220559994 2216 118-119 -1.6757546177868683 2216 120-121 -1.8444826877294354 2216 122-123 -1.8772086499172786 2216 124-125 -2.0941269496898904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34.0 3 19.0 4 50.0 5 211.0 6 714.0 7 1187.0 8 1961.0 9 3968.0 10 8315.0 11 10967.0 12 9448.0 13 11311.0 14 15876.0 15 16222.0 16 9546.0 17 8473.0 18 8638.0 19 8285.0 20 8414.0 21 8413.0 22 8581.0 23 9274.0 24 10326.0 25 12021.0 26 14143.0 27 17034.0 28 20920.0 29 26011.0 30 32740.0 31 40994.0 32 53557.0 33 70536.0 34 99991.0 35 156762.0 36 245700.0 37 376052.0 38 169580.0 39 1086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.797769612203595 17.887378391429447 11.103048089643558 23.2118039067234 2 12.687079509375849 23.338373321543678 36.88309676109521 27.091450407985263 3 22.286802679654887 29.561487340694303 28.39569988987262 19.75601008977819 4 13.485534540791793 18.98474648715072 33.414542928888174 34.115176043169306 5 11.233504300902924 39.30137041192499 34.96861142277075 14.496513864401347 6 31.505928946146938 29.663898378096793 20.15046934011881 18.679703335637456 7 23.001883314633755 32.96955975850831 24.45651012448576 19.572046802372174 8 27.805604530640593 32.06116097665224 23.463562536731313 16.66967195597585 9 29.114040711652507 14.441082972698617 21.549460383608483 34.89541593204039 10-11 23.517054705688288 27.444071491253265 26.65140868115708 22.387465121901375 12-13 27.37911891954708 25.16613259351178 26.769687797619447 20.6850606893217 14-15 27.57601176491017 22.904108235036404 26.61560480160071 22.904275198452716 16-17 22.01940155120247 26.756456136472696 32.43159852927279 18.792543783052043 18-19 23.991625260458406 24.689052732809746 33.171682160602664 18.147639846129188 20-21 20.599228174663466 25.137784212809283 34.082830475914434 20.180157136612817 22-23 19.505563124432335 26.615777101031146 29.096142544211144 24.782517230325375 24-25 20.71649436341294 28.034340439172944 27.848446599348186 23.40071859806593 26-27 22.85739568306887 23.639472137628896 29.287512688999307 24.215619490302934 28-29 21.942707775058544 23.20816958664208 32.44029402444371 22.408828613855665 30-31 24.272786771384304 25.003138857722924 30.93020382539937 19.7938705454934 32-33 18.955428220334454 25.30209835977988 32.77792247689266 22.964550942993 34-35 17.43042249721009 26.87919196398459 33.83116540522346 21.85922013358186 36-37 23.336372014745955 24.90950739969012 30.44778810706844 21.306332478495484 38-39 24.83694635892504 23.48303013303414 30.09994256558209 21.580080942458725 40-41 21.25956433961395 26.024353545141814 30.290431352801832 22.425650762442405 42-43 24.4472939039376 24.940795800609074 29.4539055404178 21.158004755035527 44-45 26.10384276326334 22.70038601271571 29.288981941550464 21.90678928247048 46-47 22.072914997061492 24.897386066143078 34.1715085216648 18.85819041513063 48-49 24.397940375060106 24.710390286904953 31.979250146925253 18.912419191109684 50-51 20.46585323502698 25.838542501469252 32.300415397766734 21.39518886573703 52-53 19.632820430624566 27.3401186087514 27.386266495699097 25.64079446492493 54-55 20.868595394561094 28.26397793449805 26.31234305711385 24.555083613827 56-57 23.109138750868194 24.275959021210664 27.43278169578458 25.182120532136558 58-59 22.458693968050436 23.616131057327564 30.41763503766629 23.50753993695571 60-61 24.431399262702357 25.523087300315222 29.074938558529677 20.970574878452744 62-63 19.43533953090773 25.851331677085003 30.836872896297486 23.876455895709782 64-65 17.789743281508787 26.986062136026074 32.21102473687022 23.013169845594913 66-67 23.28531548859326 25.446419030827588 28.936995779238128 22.33126970134103 68-69 24.55858978468772 24.121587327028905 28.632292835390288 22.68753005289309 70-71 21.265660896511193 26.25450793396378 28.894554415771758 23.585276753753273 72-73 24.06852727467009 25.403777314740612 28.201902014211676 22.325793396377623 74-75 25.653416680023504 23.10643399048993 28.18881230966501 23.051337019821553 76-77 21.74129801784474 25.005643265480582 33.20019901693648 20.052859699738207 78-79 23.690495271678152 24.82936635144521 31.3897793449805 20.09035903189614 80-81 19.93588716140407 26.017023294331352 31.628198963509107 22.418890580755463 82-83 19.183830207832454 27.167314473473315 27.123437249559224 26.52541806913501 84-85 20.4269848266282 27.92090746380296 26.080735427686058 25.57137228188278 86-87 22.404231447347332 24.0230472297911 27.359319068226746 26.213402254634826 88-89 21.768612758454882 23.430604530640593 30.051891328738577 24.748891382165947 90-91 23.47575065448523 25.272913661377356 28.655901052519102 22.595434631618314 92-93 18.954593417748573 25.567064700539614 30.11977747502271 25.358564406689105 94-95 17.477478500307544 26.606090638976802 31.48859282450895 24.427838036206705 96-97 22.491414649393114 25.054564227505445 28.569615806395166 23.88440531670627 98-99 23.603399573433745 23.984150195133857 28.1445570059016 24.267893225530795 100-101 20.60843751669605 25.682000320564192 28.60845087353743 25.101111289202326 102-103 23.03669432195565 24.742895974594312 28.21734419063616 24.003065512813876 104-105 24.29009628371834 22.62690760530489 28.398887400018967 24.6841087109578 106-107 20.796316390452727 24.415568974667 32.79733701774442 21.99077761713585 108-109 22.55057460553795 24.565275983996745 30.713015853294817 22.171133557170485 110-111 19.012831876435662 25.5703074747634 31.287308859411766 24.129551789389172 112-113 18.398784891699265 26.43885835124792 27.163802169521368 27.998554587531448 114-115 19.23883798975247 27.324217588630304 26.333237793059766 27.103706628557454 116-117 21.0074323599789 23.877320796076607 27.374461797789966 27.740785046154524 118-119 20.3002433502848 23.43831307418276 29.676978047706807 26.584465527825635 120-121 21.857898860441676 24.915690317461273 28.596862457494492 24.629548364602556 122-123 17.97845146403485 25.34970038084775 29.568812225863923 27.103035929253483 124-125 16.619171235333127 26.420939307584064 30.732961250540125 26.22692820654268 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1934.0 1 1495.5 2 3293.0 3 4454.0 4 2211.0 5 1119.5 6 1689.5 7 3619.5 8 5469.0 9 5436.0 10 4133.0 11 2796.0 12 2320.5 13 2364.0 14 2657.5 15 3077.0 16 3289.5 17 3625.0 18 4054.0 19 4063.5 20 4076.5 21 4422.0 22 4895.0 23 5529.0 24 6221.5 25 6970.0 26 8012.0 27 9189.5 28 10068.5 29 11071.0 30 12576.5 31 14215.0 32 15799.5 33 17438.0 34 19227.5 35 20329.5 36 21733.0 37 23598.5 38 24282.0 39 24451.5 40 24817.5 41 26319.0 42 28177.0 43 29838.0 44 32024.5 45 34365.5 46 37702.0 47 47298.5 48 59035.5 49 93931.5 50 221854.0 51 222464.5 52 84031.5 53 36722.0 54 28888.5 55 24934.5 56 21439.0 57 18606.5 58 16664.5 59 14709.5 60 13063.0 61 11414.0 62 9719.0 63 7781.5 64 6037.0 65 4742.0 66 3329.5 67 2577.0 68 1968.0 69 1266.5 70 997.0 71 894.0 72 877.0 73 867.0 74 673.5 75 456.5 76 278.5 77 149.0 78 86.5 79 65.0 80 31.0 81 7.0 82 5.5 83 3.5 84 2.0 85 1.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1465245498744457 2 1.335684137415184E-4 3 2.003526206122776E-4 4 0.0 5 0.0 6 0.003005289309184164 7 0.0 8 0.0 9 0.0 10-11 1.335684137415184E-4 12-13 0.004140620825987071 14-15 0.0017363893786397392 16-17 3.673131377891756E-4 18-19 0.0 20-21 3.33921034353796E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.001763103061388E-4 30-31 0.0 32-33 0.0 34-35 6.67842068707592E-5 36-37 0.0 38-39 0.0 40-41 6.67842068707592E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.681946893198696E-4 96-97 0.0028049366885718866 98-99 0.0036397392744563764 100-101 0.0 102-103 0.003840091895068654 104-105 0.0217716514398675 106-107 0.016128385959288345 108-109 0.019334027889084792 110-111 0.015293583373403858 112-113 0.01399129133942405 114-115 0.040671581984292354 116-117 0.039703210984666344 118-119 0.022773414542928888 120-121 0.013423625581022601 122-123 0.002838328792007266 124-125 0.0015360367580274617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1497360.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.944356845238794 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7145100497805 45.91043963735625 2 9.177922077365675 9.718383631236872 3 2.0490049108213686 3.254497415285197 4 0.7634491502141595 1.6168129696853113 5 0.3659081299644255 0.9686385302705279 6 0.21586631484126548 0.6857341922293577 7 0.13477418459929721 0.4994872766065895 8 0.09307239185353296 0.39421263413866764 9 0.0663552162344065 0.3161820822171661 >10 0.358062726895748 3.4500289466955705 >50 0.030826564719222353 1.1298782963109186 >100 0.021626110215043706 2.5609393733173857 >500 0.003392330162103591 1.2072431848870224 >1k 0.0019788592612270946 2.4971045495481135 >5k 0.0021202063513147446 8.064402435519174 >10k+ 0.001130776720701197 17.726014844695904 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 67263 4.492106106747876 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 57988 3.8726825880215845 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 35536 2.373243575359299 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 18263 1.2196799700806753 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17461 1.1661190361703264 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15464 1.0327509750494204 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 13468 0.899449698135385 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11424 0.7629427792915531 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9138 0.6102740823849976 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8950 0.5977186514932948 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8798 0.5875674520489395 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7798 0.5207832451781803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7747 0.5173772506277715 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7621 0.5089624405620559 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7546 0.5039536250467489 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7393 0.49373564139552284 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7347 0.49066356787946785 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6353 0.4242800662499332 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6255 0.41773521397659885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6180 0.4127263984612919 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5912 0.39482823101992837 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 5374 0.3588983277234599 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5350 0.35729550675856175 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 4916 0.32831116097665225 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3936 0.26286263824330824 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3359 0.22432815087888017 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3096 0.20676390447187049 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3076 0.20542822033445532 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2260 0.1509323075279158 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2138 0.1427846342896832 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2092 0.13971256077362826 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2067 0.13804295560185928 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.67842068707592E-5 0.0 0.0 0.0 0.0 8 6.67842068707592E-5 0.0 0.0 6.67842068707592E-5 0.0 9 1.335684137415184E-4 0.0 0.0 6.67842068707592E-5 0.0 10-11 1.335684137415184E-4 0.0 0.0 5.008815515306941E-4 0.0 12-13 1.335684137415184E-4 0.0 0.0 0.0012021157236736658 0.0 14-15 1.335684137415184E-4 0.0 0.0 0.0017029972752043597 0.0 16-17 1.335684137415184E-4 0.0 0.0 0.0034059945504087193 0.0 18-19 2.003526206122776E-4 0.0 0.0 0.004875247101565422 0.0 20-21 2.003526206122776E-4 0.0 0.0 0.007246086445477373 0.0 22-23 2.003526206122776E-4 0.0 0.0 0.011119570443981408 0.0 24-25 2.003526206122776E-4 0.0 0.0 0.014625741304696266 0.0 26-27 3.33921034353796E-4 0.0 0.0 0.01776459902762195 0.0 28-29 3.33921034353796E-4 0.0 0.0 0.02437623550782711 0.0 30-31 3.33921034353796E-4 0.0 0.0 0.0340599455040872 0.0 32-33 3.33921034353796E-4 0.0 0.0 0.05349414970347812 0.0 34-35 3.673131377891756E-4 0.0 0.0 0.08501629534647646 0.0 36-37 4.007052412245552E-4 0.0 0.0 0.1301290270876743 0.0 38-39 4.007052412245552E-4 6.67842068707592E-5 0.0 0.19818213388897793 0.0 40-41 4.007052412245552E-4 6.67842068707592E-5 0.0 0.29936020729817814 0.0 42-43 4.674894480953144E-4 6.67842068707592E-5 0.0 0.38287385799006246 0.0 44-45 4.674894480953144E-4 6.67842068707592E-5 0.0 0.4705949137148047 0.0 46-47 4.674894480953144E-4 6.67842068707592E-5 0.0 0.5819909707752311 0.0 48-49 4.674894480953144E-4 6.67842068707592E-5 0.0 0.6808649890473901 0.0 50-51 4.674894480953144E-4 6.67842068707592E-5 0.0 0.7828444729390394 0.0 52-53 4.674894480953144E-4 6.67842068707592E-5 0.0 0.9114374632686861 0.0 54-55 4.674894480953144E-4 6.67842068707592E-5 0.0 1.04417107442432 0.0 56-57 4.674894480953144E-4 6.67842068707592E-5 0.0 1.195904792434685 0.0 58-59 4.674894480953144E-4 6.67842068707592E-5 0.0 1.3813979270182188 0.0 60-61 4.674894480953144E-4 6.67842068707592E-5 0.0 1.5492266388844367 0.0 62-63 4.674894480953144E-4 1.335684137415184E-4 0.0 1.7136493562002457 0.0 64-65 4.674894480953144E-4 1.335684137415184E-4 0.0 1.9068894587807874 0.0 66-67 4.674894480953144E-4 1.335684137415184E-4 0.0 2.1264425388684085 0.0 68-69 4.674894480953144E-4 1.335684137415184E-4 0.0 2.394080247902976 0.0 70-71 4.674894480953144E-4 1.335684137415184E-4 0.0 2.782263450339264 0.0 72-73 4.674894480953144E-4 1.335684137415184E-4 0.0 3.088602607255436 0.0 74-75 4.674894480953144E-4 1.335684137415184E-4 0.0 3.3735708179729658 0.0 76-77 4.674894480953144E-4 1.335684137415184E-4 0.0 3.714737938772239 0.0 78-79 4.674894480953144E-4 1.335684137415184E-4 0.0 4.054836512261581 0.0 80-81 5.342736549660736E-4 1.335684137415184E-4 0.0 4.40542020622963 0.0 82-83 5.342736549660736E-4 1.335684137415184E-4 0.0 4.8501362397820165 0.0 84-85 5.342736549660736E-4 1.335684137415184E-4 0.0 5.275484853341881 0.0 86-87 5.342736549660736E-4 1.335684137415184E-4 0.0 5.710116471656782 0.0 88-89 5.342736549660736E-4 1.335684137415184E-4 0.0 6.211832825773362 0.0 90-91 5.342736549660736E-4 1.335684137415184E-4 0.0 6.679556018592724 0.0 92-93 5.342736549660736E-4 1.335684137415184E-4 0.0 7.1542581610300795 0.0 94-95 5.342736549660736E-4 1.335684137415184E-4 0.0 7.684524763583908 0.0 96-97 5.342736549660736E-4 1.335684137415184E-4 0.0 8.207278142864777 0.0 98-99 5.342736549660736E-4 1.335684137415184E-4 0.0 8.821893198696372 0.0 100-101 5.342736549660736E-4 1.335684137415184E-4 0.0 9.70037265587434 0.0 102-103 5.342736549660736E-4 1.335684137415184E-4 0.0 10.422042795319761 0.0 104-105 5.342736549660736E-4 1.335684137415184E-4 0.0 11.076928727894428 0.0 106-107 5.342736549660736E-4 1.335684137415184E-4 0.0 11.780433563071005 0.0 108-109 5.342736549660736E-4 1.335684137415184E-4 0.0 12.432547951060533 0.0 110-111 5.342736549660736E-4 2.003526206122776E-4 0.0 13.116718758348025 0.0 112-113 5.342736549660736E-4 2.003526206122776E-4 0.0 13.948916760164556 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1740 0.0 62.227863 5 AAGTACT 2025 0.0 53.176075 4 AAAGTAC 2035 0.0 50.86834 3 ATAACGC 130 1.8189894E-11 45.763515 3 TATAACG 125 6.2391337E-10 42.83465 2 AAAAGTA 2390 0.0 42.81474 2 TATCACG 85 3.484589E-6 41.994755 2 AAAAAGT 2600 0.0 39.191933 1 GTTATCA 740 0.0 38.65302 1 TAACGCA 215 3.6379788E-12 33.205154 4 ATCACGC 145 4.0719333E-6 28.72055 3 TTATCAA 960 0.0 28.506857 2 TCACGCA 155 6.8397694E-6 26.867613 4 TCAAACG 135 8.2548344E-5 26.441141 3 ACACCGT 160 8.745328E-6 26.030605 6 GTGTTAT 390 0.0 22.91926 1 GTCGGGA 340 1.0713848E-9 20.997377 2 CGCATGT 145 0.0038341708 20.5483 1 GTAATAC 145 0.0038707145 20.514679 3 GTATAAC 360 2.1409505E-9 19.863358 1 >>END_MODULE