##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364134_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330146 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.079102594752758 31.0 30.0 34.0 26.0 34.0 2 30.105951527125594 31.0 31.0 34.0 26.0 34.0 3 30.27726430030989 31.0 31.0 34.0 26.0 34.0 4 33.54042488568924 37.0 35.0 37.0 27.0 37.0 5 33.66736959702168 37.0 35.0 37.0 28.0 37.0 6 33.48705781169887 37.0 35.0 37.0 27.0 37.0 7 33.444951907534964 37.0 35.0 37.0 27.0 37.0 8 33.24971995555375 37.0 35.0 37.0 26.0 37.0 9 34.57911387171033 39.0 35.0 39.0 25.0 39.0 10-11 34.60425434501175 39.0 35.0 39.0 25.5 39.0 12-13 34.51288730710764 39.0 35.0 39.0 24.0 39.0 14-15 35.655680654604836 40.0 36.0 41.0 25.0 41.0 16-17 35.716486385705025 40.0 36.0 41.0 25.0 41.0 18-19 35.672128097216394 40.0 36.0 41.0 25.0 41.0 20-21 35.67110151817921 40.0 36.0 41.0 25.0 41.0 22-23 35.65982380881498 40.0 36.0 41.0 25.5 41.0 24-25 35.63263318462785 40.0 36.0 41.0 25.5 41.0 26-27 35.46607176956515 40.0 35.0 41.0 25.0 41.0 28-29 35.3843615663243 40.0 35.0 41.0 25.0 41.0 30-31 35.44226160135804 40.0 35.0 41.0 25.0 41.0 32-33 35.19025693420121 40.0 35.0 41.0 24.5 41.0 34-35 35.2527715002714 40.0 35.0 41.0 25.0 41.0 36-37 35.24116863863065 40.0 35.0 41.0 25.0 41.0 38-39 35.12020898457763 40.0 35.0 41.0 25.0 41.0 40-41 34.889293731665546 40.0 35.0 41.0 24.0 41.0 42-43 34.70240597648679 39.0 34.0 41.0 23.5 41.0 44-45 34.50276999667706 39.0 34.0 41.0 23.0 41.0 46-47 34.295977659595266 39.0 33.0 41.0 22.0 41.0 48-49 34.094334381338584 39.0 33.0 41.0 19.0 41.0 50-51 33.99353792741549 39.0 33.0 41.0 18.5 41.0 52-53 33.86565572501064 38.5 33.0 41.0 18.0 41.0 54-55 33.63065934115503 38.0 33.0 40.0 14.5 41.0 56-57 33.3265671587931 38.0 32.5 40.0 12.0 41.0 58-59 33.04275921590562 38.0 32.0 40.0 8.0 41.0 60-61 32.7821115125708 37.0 32.0 40.0 7.5 41.0 62-63 32.33041372901922 37.0 31.0 40.0 4.0 41.0 64-65 32.12305641636331 36.0 31.0 40.0 2.0 41.0 66-67 31.756901122132458 36.0 31.0 39.0 2.0 41.0 68-69 31.380318777036507 35.0 30.5 39.0 2.0 41.0 70-71 30.953018315282684 35.0 30.0 39.0 2.0 40.5 72-73 30.660028297645525 35.0 30.0 38.0 2.0 40.0 74-75 30.36388599446978 35.0 30.0 37.0 2.0 39.5 76-77 29.9403952648807 35.0 29.5 37.0 2.0 39.0 78-79 29.57701071912407 35.0 29.0 36.0 2.0 39.0 80-81 29.316045005585856 35.0 29.0 36.0 2.0 37.5 82-83 29.130657461662103 35.0 29.0 35.5 2.0 37.0 84-85 28.840971216693504 34.0 29.0 35.0 2.0 37.0 86-87 28.51602944338441 34.0 29.0 35.0 2.0 36.0 88-89 28.274611959890116 34.0 28.0 35.0 2.0 36.0 90-91 28.112719205260177 34.0 28.0 35.0 2.0 36.0 92-93 27.83388928734139 34.0 27.0 35.0 2.0 36.0 94-95 27.78297156853458 34.0 27.0 35.0 2.0 35.0 96-97 27.710333677656436 34.0 27.0 35.0 2.0 35.0 98-99 27.53277422177716 34.0 27.0 35.0 2.0 35.0 100-101 27.18747453287083 34.0 25.0 35.0 2.0 35.0 102-103 26.010384198426337 32.5 23.5 34.5 2.0 35.0 104-105 26.77593512291132 34.0 25.0 35.0 2.0 35.0 106-107 26.833615633171092 34.0 25.0 35.0 2.0 35.0 108-109 26.806976452209007 34.0 25.0 35.0 2.0 35.0 110-111 26.7755423088894 34.0 25.0 35.0 2.0 35.0 112-113 26.689611140431204 34.0 25.0 35.0 2.0 35.0 114-115 26.50931175976171 34.0 24.5 35.0 2.0 35.0 116-117 26.235892150185016 34.0 24.0 35.0 2.0 35.0 118-119 26.034272553539235 34.0 23.5 35.0 2.0 35.0 120-121 25.848909067124964 34.0 21.5 35.0 2.0 35.0 122-123 25.46199364581031 34.0 18.0 35.0 2.0 35.0 124-125 24.583138993764592 32.5 2.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.621817864476462 1101 2 0.6825529765611194 1101 3 0.6231037009604279 1101 4 0.7200413251204694 1101 5 0.5717176602536256 1101 6 0.5517616941560846 1101 7 0.5858833490143951 1101 8 0.7052984897984658 1101 9 0.8849349042215024 1101 10-11 0.7956931335358206 1101 12-13 0.867025878368274 1101 14-15 0.9539078270932606 1101 16-17 0.8668940685857791 1101 18-19 0.9028810190453598 1101 20-21 0.8793848694641895 1101 22-23 0.8927288868291967 1101 24-25 0.8981902915876887 1101 26-27 0.9470264199756144 1101 28-29 1.0499895140485123 1101 30-31 0.9352049688242587 1101 32-33 0.9701626892058499 1101 34-35 0.9764885449179843 1101 36-37 0.8860821057845527 1101 38-39 0.8583268342069417 1101 40-41 0.8205882758076015 1101 42-43 0.8502316307901552 1101 44-45 0.7487966329864122 1101 46-47 0.6881185296265215 1101 48-49 0.7653600521644179 1101 50-51 0.7508491839123366 1101 52-53 0.8577229521125815 1101 54-55 0.8385311630610275 1101 56-57 0.8684307501877271 1101 58-59 1.033499828252161 1101 60-61 0.9110352489498581 1101 62-63 1.1002341919608796 1101 64-65 0.9067645672829059 1101 66-67 0.817137051736534 1101 68-69 0.8086908954542658 1101 70-71 0.9054893660146348 1101 72-73 0.6883681505746111 1101 74-75 0.6975697476486751 1101 76-77 0.6690117877670723 1101 78-79 0.5960102912070013 1101 80-81 0.6853331614094387 1101 82-83 0.6940802117051064 1101 84-85 0.7478182966581599 1101 86-87 0.5521927774102515 1101 88-89 0.6133836242869464 1101 90-91 0.6015132744065994 1101 92-93 0.6563939938389503 1101 94-95 0.5093653755419005 1101 96-97 0.5174892201479615 1101 98-99 0.4923984165794053 1101 100-101 0.5331098344776528 1101 102-103 0.4606510947697622 1101 104-105 0.5613343581997796 1101 106-107 0.6121732876023565 1101 108-109 0.5808983225250763 1101 110-111 0.47369172799323067 1101 112-113 0.7261793444714115 1101 114-115 0.7357260621449662 1101 116-117 0.5828974031826348 1101 118-119 0.6393790452931292 1101 120-121 0.7025552598942042 1101 122-123 0.7876483423920497 1101 124-125 0.6538105161649739 1102 1 0.3956340761105892 1102 2 0.45800986319467896 1102 3 0.45723724540504307 1102 4 0.4757065291227818 1102 5 0.3062358873627886 1102 6 0.2833685092594962 1102 7 0.44255747962047565 1102 8 0.40396021268426097 1102 9 0.45797863247773307 1102 10-11 0.3794305800863853 1102 12-13 0.3562235568488248 1102 14-15 0.40582672188766367 1102 16-17 0.4638418687495047 1102 18-19 0.3326296620335327 1102 20-21 0.46581374703531964 1102 22-23 0.43921952290586574 1102 24-25 0.4448907353547398 1102 26-27 0.4718245527400171 1102 28-29 0.5920393087593538 1102 30-31 0.5321787967682141 1102 32-33 0.6004219523210779 1102 34-35 0.428384979191371 1102 36-37 0.47711327623137123 1102 38-39 0.42875998931824455 1102 40-41 0.4965067393599938 1102 42-43 0.5044618188930556 1102 44-45 0.5442053325539504 1102 46-47 0.5051642418073641 1102 48-49 0.47119896847862464 1102 50-51 0.4649309793165415 1102 52-53 0.5530301301251654 1102 54-55 0.45715270539593433 1102 56-57 0.3787972054788753 1102 58-59 0.42710328326162994 1102 60-61 0.462764867612492 1102 62-63 0.5461369701423564 1102 64-65 0.49143095455830377 1102 66-67 0.5529997392092163 1102 68-69 0.5610666900942824 1102 70-71 0.3153060141755972 1102 72-73 0.4705526238542532 1102 74-75 0.4569981458858692 1102 76-77 0.3547098264086834 1102 78-79 0.27128783718633187 1102 80-81 0.34969264812575673 1102 82-83 0.27752382179257395 1102 84-85 0.1948434402865864 1102 86-87 0.10443964865967459 1102 88-89 0.08093662715290861 1102 90-91 0.1541468629795304 1102 92-93 0.1573130255103976 1102 94-95 0.06966090843493689 1102 96-97 0.14483693913672013 1102 98-99 0.05223786877522585 1102 100-101 0.14426510326666886 1102 102-103 0.11265333130917199 1102 104-105 0.0516726347855041 1102 106-107 0.020806926909642698 1102 108-109 0.12640875504072469 1102 110-111 0.15603086177813452 1102 112-113 0.08418881911189757 1102 114-115 -0.03177015523905169 1102 116-117 0.03946575794682872 1102 118-119 -0.039362441508803414 1102 120-121 0.003403369167546799 1102 122-123 0.03475067094032269 1102 124-125 0.05672834923732495 1103 1 0.21479570096201783 1103 2 0.20218361601954626 1103 3 0.349824304974625 1103 4 0.285198863357742 1103 5 0.2582492351680514 1103 6 0.2717016854024834 1103 7 0.2804701759282011 1103 8 0.20419386180518018 1103 9 0.3334382839737131 1103 10-11 0.277224852139625 1103 12-13 0.32362926999711306 1103 14-15 0.3177411596212636 1103 16-17 0.3802242466868577 1103 18-19 0.44621353941047204 1103 20-21 0.43914731658840367 1103 22-23 0.44891486717096285 1103 24-25 0.47934147493966606 1103 26-27 0.6423669167542982 1103 28-29 0.6197968660109439 1103 30-31 0.44930331288682623 1103 32-33 0.39786773077022985 1103 34-35 0.4165128525831392 1103 36-37 0.44815464577788333 1103 38-39 0.5078923455007995 1103 40-41 0.5626936888789587 1103 42-43 0.5977353139431258 1103 44-45 0.5551048764587776 1103 46-47 0.566381327581702 1103 48-49 0.6574542879823397 1103 50-51 0.6153371296450558 1103 52-53 0.7096162059089721 1103 54-55 0.8294725014271336 1103 56-57 0.7906131604206479 1103 58-59 0.8083252202253419 1103 60-61 0.6281600728032117 1103 62-63 0.6373834913289187 1103 64-65 0.6549303660063828 1103 66-67 0.5625723707892583 1103 68-69 0.47058953315179153 1103 70-71 0.5597612766739211 1103 72-73 0.5033242057849066 1103 74-75 0.4745607801598588 1103 76-77 0.5258393093948648 1103 78-79 0.5851510617838933 1103 80-81 0.43449232422679884 1103 82-83 0.4411252332750415 1103 84-85 0.4487781925711012 1103 86-87 0.37381118775812183 1103 88-89 0.46586625885332467 1103 90-91 0.38231182113476336 1103 92-93 0.34164571601337457 1103 94-95 0.5160949976540898 1103 96-97 0.361256521684318 1103 98-99 0.4054241257882154 1103 100-101 0.4478177266867114 1103 102-103 0.3290637950797475 1103 104-105 0.4040607382726833 1103 106-107 0.4423011609766654 1103 108-109 0.46833384350588503 1103 110-111 0.4190058393329146 1103 112-113 0.4401211306906063 1103 114-115 0.45572708958760444 1103 116-117 0.40220953542365834 1103 118-119 0.42098924627260814 1103 120-121 0.2600357162313287 1103 122-123 0.3590580216646373 1103 124-125 0.4894743725386661 1104 1 0.41165662190103447 1104 2 0.3981202044218364 1104 3 0.4073069801297997 1104 4 0.4404129679370712 1104 5 0.41032299481091883 1104 6 0.4608417396744571 1104 7 0.4245511491137961 1104 8 0.32905495022926345 1104 9 0.5500191104881509 1104 10-11 0.48293631189123687 1104 12-13 0.40028606364651154 1104 14-15 0.5407648054448799 1104 16-17 0.5275405054697799 1104 18-19 0.5169702578619919 1104 20-21 0.39724050888048623 1104 22-23 0.4220347317028086 1104 24-25 0.4615833111991563 1104 26-27 0.5802015977987196 1104 28-29 0.5729714017571936 1104 30-31 0.5442331407837528 1104 32-33 0.5530707589391213 1104 34-35 0.6034090208511032 1104 36-37 0.5687055499172757 1104 38-39 0.6973789331849503 1104 40-41 0.7301069380672871 1104 42-43 0.8141754335294067 1104 44-45 0.6417646424811707 1104 46-47 0.6502060194079746 1104 48-49 0.7215214631252138 1104 50-51 0.7089999865945558 1104 52-53 0.6866053892464521 1104 54-55 0.8556168467735432 1104 56-57 0.8751091314067878 1104 58-59 0.7390820750019174 1104 60-61 0.694330061865557 1104 62-63 0.8034508957900144 1104 64-65 0.8784581710829826 1104 66-67 0.8668596232527896 1104 68-69 0.8229873569170998 1104 70-71 0.7750137859677579 1104 72-73 0.7463429633816787 1104 74-75 0.7580825625904346 1104 76-77 0.7045601130115706 1104 78-79 0.6978861585953702 1104 80-81 0.6887402493473438 1104 82-83 0.682424956316094 1104 84-85 0.629405355970551 1104 86-87 0.6413297480487294 1104 88-89 0.6665798231229161 1104 90-91 0.6864569060445795 1104 92-93 0.7466675657544428 1104 94-95 0.7958893853951601 1104 96-97 0.6301757001603434 1104 98-99 0.5879516847494131 1104 100-101 0.6823060659043385 1104 102-103 0.47473843246119785 1104 104-105 0.5550728665769533 1104 106-107 0.6361220332671138 1104 108-109 0.6751126553795039 1104 110-111 0.5398373052970555 1104 112-113 0.5520226971871693 1104 114-115 0.6488120500375132 1104 116-117 0.5300922929581091 1104 118-119 0.7320426031674536 1104 120-121 0.6358732936962106 1104 122-123 0.7550983631686314 1104 124-125 0.836674316840142 1105 1 0.3198052193505987 1105 2 0.3142672090349663 1105 3 0.29224178525465305 1105 4 0.3403200857874822 1105 5 0.3886483281549147 1105 6 0.3888898005659769 1105 7 0.37095405179339025 1105 8 0.08296396969054598 1105 9 0.3175951461940727 1105 10-11 0.26807726642395124 1105 12-13 0.48170708558998143 1105 14-15 0.4455439892511066 1105 16-17 0.5040294303101831 1105 18-19 0.37330008392405745 1105 20-21 0.38055120042031376 1105 22-23 0.2873262612404872 1105 24-25 0.5464916606498846 1105 26-27 0.5159372928734598 1105 28-29 0.5374854492455015 1105 30-31 0.43378170056083576 1105 32-33 0.3146253183334835 1105 34-35 0.415791575395005 1105 36-37 0.5040887524877959 1105 38-39 0.5141190545263115 1105 40-41 0.528539682947148 1105 42-43 0.567167622735191 1105 44-45 0.5384834643817697 1105 46-47 0.4576000930371862 1105 48-49 0.297655597140313 1105 50-51 0.28994410691466044 1105 52-53 0.33474892039694737 1105 54-55 0.43641644766508847 1105 56-57 0.4835797686063046 1105 58-59 0.24055449939589835 1105 60-61 0.18677008442907805 1105 62-63 0.26583387502368794 1105 64-65 0.22500889078224162 1105 66-67 0.3069037959846064 1105 68-69 0.2916840989470444 1105 70-71 0.2155509735522294 1105 72-73 0.0801838426193946 1105 74-75 0.07000083129514323 1105 76-77 -0.09685307965118994 1105 78-79 -0.2117588457289692 1105 80-81 -0.0558415836236712 1105 82-83 -0.099728055908475 1105 84-85 -0.045334121840905794 1105 86-87 -0.04433774684624936 1105 88-89 -0.2602836031441953 1105 90-91 -0.2587377542560745 1105 92-93 -0.3017949074089117 1105 94-95 -0.40879271932734085 1105 96-97 -0.2566734565306916 1105 98-99 -0.23899535543773354 1105 100-101 -0.31379128498084086 1105 102-103 -0.5609052798069882 1105 104-105 -0.27987407967880884 1105 106-107 -0.2721058812413375 1105 108-109 -0.2770116927841606 1105 110-111 -0.16604954435927155 1105 112-113 -0.22587542323586263 1105 114-115 -0.15968884006226602 1105 116-117 -0.1304582998338546 1105 118-119 -0.2373669931224569 1105 120-121 -0.15790272791999627 1105 122-123 -0.17982005392767775 1105 124-125 0.008147680547953229 1106 1 0.22571039958195627 1106 2 0.2619703041653558 1106 3 0.35902776533758285 1106 4 0.2936768440204176 1106 5 0.15855862420089295 1106 6 0.23374083946826119 1106 7 0.28715235944689255 1106 8 0.3524276221820557 1106 9 0.43745561829161517 1106 10-11 0.49661961365309537 1106 12-13 0.5051110514928894 1106 14-15 0.4425383960438438 1106 16-17 0.3700337581142392 1106 18-19 0.2807625231294608 1106 20-21 0.28570797908315626 1106 22-23 0.4202410247643513 1106 24-25 0.4901233678601855 1106 26-27 0.5736072102574354 1106 28-29 0.5626890186415139 1106 30-31 0.4982023460207117 1106 32-33 0.3083324389887423 1106 34-35 0.3733786787056914 1106 36-37 0.3640029364694968 1106 38-39 0.2951485340432427 1106 40-41 0.3234760896606659 1106 42-43 0.3001065685938613 1106 44-45 0.2528822727763753 1106 46-47 0.27705420809923 1106 48-49 0.21247749215979894 1106 50-51 0.27649659196226395 1106 52-53 0.1891702225322831 1106 54-55 0.26446258797683697 1106 56-57 0.32796774706735476 1106 58-59 0.30507833671549633 1106 60-61 0.34572437564693104 1106 62-63 0.12202246804957895 1106 64-65 0.11854668297677051 1106 66-67 0.07602764497843495 1106 68-69 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2216 96-97 -2.0341170189221565 2216 98-99 -2.118983483162154 2216 100-101 -2.312565664492009 2216 102-103 -2.149302050075505 2216 104-105 -2.3198485040801735 2216 106-107 -2.408932380632958 2216 108-109 -2.335497416520443 2216 110-111 -2.319441116815593 2216 112-113 -2.3444150742736554 2216 114-115 -2.362895240459192 2216 116-117 -2.4680178088768656 2216 118-119 -2.389490122205398 2216 120-121 -2.244449254245737 2216 122-123 -2.2480058993400185 2216 124-125 -2.417657980342053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50494.0 3 9140.0 4 6971.0 5 4939.0 6 5533.0 7 4816.0 8 4446.0 9 4951.0 10 5996.0 11 6970.0 12 7664.0 13 8257.0 14 8699.0 15 8282.0 16 8082.0 17 8074.0 18 7952.0 19 8148.0 20 8600.0 21 9294.0 22 10956.0 23 15136.0 24 18564.0 25 16840.0 26 20174.0 27 21460.0 28 24041.0 29 29756.0 30 34450.0 31 41374.0 32 52042.0 33 65330.0 34 81859.0 35 108783.0 36 160269.0 37 288126.0 38 152633.0 39 1045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.05089630591572 18.76716292302474 10.855541730785648 22.3263990402739 2 13.691961274406497 24.04577759749947 37.69158661781024 24.570674510283794 3 20.755805698300055 32.05573228684243 26.98135409397271 20.207107920884802 4 12.602071796916775 19.684034354731025 33.85014969019718 33.863744158155015 5 11.303099993580231 42.75136231292979 32.47912630708403 13.46641138640595 6 29.397084748937637 33.881673859875185 20.01194923614017 16.709292155047002 7 25.314525611775824 35.32935036178281 21.737101221399296 17.619022805042075 8 27.341847884795957 35.108738431740186 21.137838587129366 16.411575096334495 9 29.76909094480515 17.847477935810243 19.04372645693158 33.33970466245303 10-11 22.831420402023454 31.63550054983475 24.69704313190103 20.836035916240768 12-13 25.46534952684963 29.317416844530907 24.546210074986305 20.67102355363315 14-15 25.578953649717462 28.479103736922134 23.208225413306323 22.73371720005408 16-17 21.775950980118154 31.803400784517528 27.359425502937974 19.061222732426348 18-19 23.094436352691986 30.60547917167812 27.764235578627854 18.53584889700204 20-21 21.84584985163272 30.689045822961596 27.60945698368583 19.855647341719852 22-23 20.77609737045087 32.122397515424716 23.77843100370405 23.323074110420364 24-25 20.935980405672083 33.16562834605819 23.189441421910672 22.708949826359053 26-27 22.43154345735475 29.856246359699124 24.105458619363716 23.606751563582414 28-29 22.144336111448943 29.715802776705523 26.347579957380233 21.792281154465293 30-31 23.90398982193293 31.115858995391843 25.062961302266757 19.91718988040847 32-33 19.637095905996688 31.485647134016364 26.980698527675816 21.896558432311124 34-35 18.372068514981184 32.307215210576636 27.548357439183263 21.772358835258917 36-37 22.71088133451989 30.659417956076556 25.488500745498598 21.141199963904956 38-39 24.38338607021654 29.38619400731875 24.954710691899855 21.27570923056485 40-41 21.040102227431884 32.12004930224343 25.11414163752586 21.725706832798824 42-43 23.347411089346572 30.786165302294332 24.658189819480143 21.20823378887895 44-45 24.54583896519697 29.411353837092523 24.752814673301568 21.289992524408937 46-47 21.750561372652893 30.581603590670507 28.40653235005488 19.26130268662172 48-49 23.08694910160197 30.2019413311424 27.41828179960521 19.292827767650422 50-51 20.50073314115119 31.007831246158744 27.634249394837664 20.8571862178524 52-53 19.800705687743555 32.13631166226949 24.30779823838535 23.755184411601608 54-55 20.554420117463103 32.754977157435135 23.483221228221044 23.20738149688071 56-57 22.20709547289565 29.863059094216794 24.054110567269454 23.875734865618103 58-59 21.99690866987878 29.47332265144781 26.239730952367474 22.290037726305936 60-61 23.42374990185242 31.082205391170948 25.14103051936678 20.35301418760985 62-63 19.468744597111566 31.506172410747112 26.79285297424054 22.232230017900783 64-65 18.1202124394175 32.08379630311316 27.46814333733158 22.32784792013776 66-67 22.251374124184586 30.790725416767334 25.410085467504228 21.547814991543852 68-69 23.744339642229527 29.58550797404965 24.950083147564747 21.72006923615607 70-71 20.771934037240158 31.79771853241241 25.121854713691448 22.30849271665598 72-73 22.970315272100255 30.764996313147957 24.661849784093565 21.602838630658223 74-75 24.077176488356507 29.43811061082321 24.764704216577467 21.72000868424282 76-77 21.30176956303259 30.63253261087471 28.259851030662585 19.805846795430117 78-79 22.640411115334942 30.378608593693667 27.2544733103837 19.726506980587686 80-81 20.16417605036655 31.274431409325636 27.37941256641832 21.181979973889494 82-83 19.716793899758116 32.016114387840894 24.157650993105214 24.109440719295776 84-85 20.320048081206497 32.658638534731274 23.49093116565214 23.53038221841009 86-87 21.769614879573883 29.778553678476584 24.34221902398102 24.109612417968513 88-89 21.77718184041965 29.351223533888398 26.302996397196278 22.56859822849567 90-91 23.05121178614397 30.83764417554034 25.234348367894942 20.876795670420744 92-93 19.583529185237204 31.16637123704782 26.49333537208086 22.756764205634116 94-95 18.30662613394223 31.625152337502886 27.349470915051928 22.718750613502955 96-97 22.211776197077366 30.344667249647966 25.63199185990031 21.811564693374354 98-99 23.47890919600861 29.245912352630004 25.05152243027398 22.223656021087407 100-101 20.67731033397641 31.24092311860232 25.219137471290736 22.862629076130535 102-103 22.625897272232056 30.320181136949735 24.998377855540113 22.055543735278096 104-105 23.413772536639783 29.27074298903061 25.11069554565727 22.204788928672343 106-107 21.00815921178935 30.253530225004678 28.168109043050936 20.570201520155035 108-109 22.30643021344487 30.33295195716914 26.947546689649865 20.413071139736125 110-111 19.97143724118774 31.125972032047056 27.311817162111772 21.590773564653436 112-113 19.305659864664435 31.89533948998192 24.411199716195355 24.387800929158285 114-115 19.848771266540645 32.98188025259637 23.744556584537246 23.424791896325743 116-117 21.268744845238302 30.355915308350017 24.34062438925742 24.034715457154263 118-119 21.04512798672182 30.126265028944072 25.845441818107677 22.983165166226428 120-121 22.108910707900346 31.492214217666604 25.099448600696356 21.299426473736695 122-123 19.33780498206887 31.851137273728458 26.073659920696922 22.737397823505745 124-125 18.4539889062237 32.589835963368955 26.452671057984567 22.503504072422782 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11033.0 1 7284.0 2 5773.0 3 7977.0 4 7005.5 5 5242.5 6 3847.0 7 3101.5 8 2906.0 9 2634.0 10 2107.0 11 1707.0 12 1530.5 13 1443.0 14 1411.0 15 1459.5 16 1512.5 17 1569.5 18 1719.0 19 1908.5 20 2177.0 21 2599.0 22 3145.5 23 3941.5 24 4831.5 25 5907.0 26 7366.0 27 8802.0 28 10027.5 29 11649.5 30 14083.0 31 16360.0 32 18483.5 33 20970.5 34 23953.5 35 26501.5 36 29481.0 37 32335.0 38 32957.0 39 32944.0 40 32095.0 41 31426.5 42 30987.0 43 31473.0 44 33073.0 45 34700.0 46 36274.5 47 39477.0 48 45801.0 49 66584.0 50 137246.5 51 140494.5 52 65599.0 53 35884.5 54 28395.0 55 24438.0 56 21348.0 57 18709.5 58 17392.0 59 16018.5 60 14049.0 61 12101.5 62 9978.0 63 7402.5 64 5481.0 65 4338.5 66 3052.5 67 2249.0 68 1661.0 69 1065.0 70 910.5 71 823.0 72 723.0 73 723.0 74 565.5 75 435.5 76 319.5 77 188.0 78 147.5 79 97.0 80 60.0 81 45.5 82 35.5 83 22.0 84 14.5 85 16.5 86 13.0 87 8.5 88 5.5 89 4.5 90 2.0 91 2.0 92 2.5 93 2.5 94 4.0 95 3.0 96 0.5 97 1.0 98 1.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4837814796270485 2 0.4711512871519367 3 0.4587466338281662 4 0.456942320617436 5 0.4594232512821901 6 0.46701640271067985 7 0.4517549201365865 8 0.4791203371659953 9 0.48926959897635297 10-11 0.493667612427508 12-13 0.4940059211545199 14-15 0.469798052243889 16-17 0.4717527248888468 18-19 0.45825796566692684 20-21 0.4687831260628533 22-23 0.4855857928377787 24-25 0.47314354965545136 26-27 0.4828793230216833 28-29 0.47716566452103754 30-31 0.4898334468547062 32-33 0.48795395392686214 34-35 0.4838190694856054 36-37 0.44089145101364813 38-39 0.4007078922163431 40-41 0.39521977286703863 42-43 0.4030384634468698 44-45 0.43874882907590596 46-47 0.44630439064583893 48-49 0.44356033097118663 50-51 0.42912582528534465 52-53 0.4226979594721181 54-55 0.4243895031071777 56-57 0.40702298845389906 58-59 0.41149618162216783 60-61 0.4217582130081961 62-63 0.422735549330675 64-65 0.43720764487507385 66-67 0.4259682771665667 68-69 0.4280357193871951 70-71 0.437771492753427 72-73 0.43946303638848666 74-75 0.4441993585666536 76-77 0.4345011750589785 78-79 0.42487817126841715 80-81 0.4341628663319666 82-83 0.43149398637442804 84-85 0.43002798189070973 86-87 0.4365686172796069 88-89 0.42266036961356124 90-91 0.4266824844791474 92-93 0.4363054882697087 94-95 0.4348394837859904 96-97 0.4019483575487202 98-99 0.37552268698323343 100-101 0.37646243344715546 102-103 0.3563518591192245 104-105 0.35909591879387676 106-107 0.3772518204768499 108-109 0.38492015162245347 110-111 0.4017604082559358 112-113 0.3977758832489065 114-115 0.39585880046250566 116-117 0.3695083096141326 118-119 0.4411545800235463 120-121 0.44878532131059296 122-123 0.4025873851441872 124-125 0.3953701323012662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1330146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.19758267137131 #Duplication Level Percentage of deduplicated Percentage of total 1 84.49821632471802 41.57107983218731 2 9.867799779530602 9.709437908759927 3 2.549150184604537 3.7623608084646945 4 0.9940618410382903 1.956217584197474 5 0.5324477870545318 1.3097572010902014 6 0.31220383377507405 0.9215804353480963 7 0.18662584937965496 0.6427078457429325 8 0.14225258019698453 0.5598786459565619 9 0.10164889622993588 0.4500791978153322 >10 0.7013089127723546 6.700266748367216 >50 0.0716410264646265 2.402048846445081 >100 0.03454081723304133 2.916649604486335 >500 0.001492504447800735 0.5024546521273376 >1k 0.0036246536589446426 5.269689845286234 >5k 0.0012792895266863444 4.526287732629357 >10k+ 0.0017057193689151257 16.79950311109593 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 46004 3.4585677061014355 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 33644 2.5293464025753565 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 22077 1.6597426147204892 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 12418 0.9335817271186773 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 12195 0.9168166502023086 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 12127 0.9117044294385729 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 11218 0.8433660665821647 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 10470 0.7871316381810718 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 9110 0.6848872229063577 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8125 0.6108352015493036 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7785 0.585274097730625 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 7614 0.5724183661041721 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 5365 0.4033391823153248 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 5151 0.38725072285298007 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4914 0.3694331298970188 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 4840 0.36386983083060054 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 4419 0.3322191699257074 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4377 0.32906162180692944 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3922 0.29485485052016847 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3307 0.2486193244952058 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3299 0.2480178867582957 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 3171 0.23839488296773437 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 3073 0.23102727069058585 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3049 0.22922295747985558 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2965 0.22290786124229972 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2361 0.17749931210558842 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 1870 0.14058607100273202 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.517971711376044E-5 0.0 5 0.0 0.0 0.0 7.517971711376044E-5 0.0 6 0.0 0.0 0.0 7.517971711376044E-5 0.0 7 0.0 0.0 0.0 7.517971711376044E-5 0.0 8 7.517971711376044E-5 0.0 0.0 7.517971711376044E-5 0.0 9 7.517971711376044E-5 0.0 0.0 2.2553915134128133E-4 0.0 10-11 7.517971711376044E-5 0.0 0.0 4.1348844412568246E-4 0.0 12-13 7.517971711376044E-5 0.0 0.0 6.766174540238439E-4 0.0 14-15 7.517971711376044E-5 0.0 0.0 0.0012404653323770474 0.0 16-17 7.517971711376044E-5 0.0 0.0 0.002368161089083454 0.0 18-19 7.517971711376044E-5 0.0 0.0 0.0034206771286761 0.0 20-21 7.517971711376044E-5 0.0 0.0 0.00503704104662195 0.0 22-23 7.517971711376044E-5 0.0 0.0 0.008608077609525571 0.0 24-25 7.517971711376044E-5 0.0 0.0 0.012630192475111754 0.0 26-27 7.517971711376044E-5 0.0 0.0 0.017216155219051142 0.0 28-29 7.517971711376044E-5 0.0 0.0 0.02244114555845749 0.0 30-31 7.517971711376044E-5 0.0 0.0 0.03161307104633627 0.0 32-33 7.517971711376044E-5 0.0 0.0 0.04946825386085437 0.0 34-35 7.517971711376044E-5 0.0 0.0 0.07311227489313205 0.0 36-37 1.5035943422752089E-4 0.0 0.0 0.10637929971597103 0.0 38-39 2.2553915134128133E-4 0.0 0.0 0.16377901373232712 0.0 40-41 2.2553915134128133E-4 0.0 0.0 0.24425890090260768 0.0 42-43 2.2553915134128133E-4 0.0 0.0 0.30872550832765727 0.0 44-45 3.758985855688022E-4 0.0 0.0 0.3760113551444728 0.0 46-47 5.262580197963232E-4 0.0 0.0 0.4692342043655358 0.0 48-49 5.262580197963232E-4 0.0 0.0 0.5585477082966832 0.0 50-51 5.262580197963232E-4 0.0 0.0 0.6515450183664049 0.0 52-53 5.262580197963232E-4 0.0 0.0 0.7667203449846859 0.0 54-55 5.262580197963232E-4 0.0 0.0 0.8841510631163797 0.0 56-57 5.262580197963232E-4 0.0 0.0 1.016504955095155 0.0 58-59 5.262580197963232E-4 0.0 0.0 1.173743333438585 0.0 60-61 5.262580197963232E-4 0.0 0.0 1.320870039830214 0.0 62-63 5.262580197963232E-4 0.0 0.0 1.47092875518928 0.0 64-65 5.262580197963232E-4 0.0 0.0 1.6486160165876527 0.0 66-67 5.262580197963232E-4 0.0 0.0 1.8502104280282015 0.0 68-69 5.262580197963232E-4 0.0 0.0 2.0934544027497735 0.0 70-71 5.262580197963232E-4 0.0 0.0 2.424921775504343 0.0 72-73 5.262580197963232E-4 0.0 0.0 2.7028235998153587 0.0 74-75 5.262580197963232E-4 0.0 0.0 2.9679072823584782 0.0 76-77 5.262580197963232E-4 0.0 0.0 3.2796775692292424 0.0 78-79 5.262580197963232E-4 0.0 0.0 3.5850575801453375 0.0 80-81 6.014377369100835E-4 0.0 0.0 3.920735017058278 0.0 82-83 6.014377369100835E-4 0.0 0.0 4.330765194196728 0.0 84-85 6.014377369100835E-4 0.0 0.0 4.725609068478198 0.0 86-87 6.014377369100835E-4 0.0 0.0 5.131692310468174 0.0 88-89 6.014377369100835E-4 0.0 0.0 5.584913235088479 0.0 90-91 6.014377369100835E-4 0.0 0.0 6.0515913290721475 0.0 92-93 6.014377369100835E-4 0.0 0.0 6.52217876834573 0.0 94-95 6.014377369100835E-4 0.0 0.0 7.02967944872217 0.0 96-97 7.517971711376044E-4 0.0 0.0 7.5437207644875075 0.0 98-99 8.269768882513649E-4 0.0 0.0 8.129333170945145 0.0 100-101 8.645667468082451E-4 0.0 0.0 8.908646118546386 0.0 102-103 9.397464639220055E-4 0.0 0.0 9.570415578440262 0.0 104-105 0.001202875473820167 0.0 0.0 10.204518902436273 0.0 106-107 0.001202875473820167 0.0 0.0 10.880685278157436 0.0 108-109 0.001202875473820167 0.0 0.0 11.512307671488694 0.0 110-111 0.001202875473820167 0.0 0.0 12.160507192443536 0.0 112-113 0.001202875473820167 0.0 0.0 12.947225342180483 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1275 0.0 34.548588 5 GTACAAA 1495 0.0 31.452984 1 GAACAAA 1840 0.0 28.466942 1 AAAGTAC 1550 0.0 26.114756 3 AAGTACT 1685 0.0 25.435562 4 ATGGGAG 2975 0.0 24.810955 5 GTATAGG 290 1.4551915E-10 24.629704 1 GTACAAG 970 0.0 24.545065 1 ATGGGAT 3095 0.0 23.271988 5 CATGGGG 3955 0.0 23.178377 4 GTCGGGA 420 0.0 22.674965 2 TCGTAGC 185 2.6832851E-5 22.521757 7 CATGGGT 2115 0.0 21.952955 4 AAAAAGT 1970 0.0 20.545591 1 TAACGCA 175 4.7697136E-4 20.409012 4 CGCAAAA 380 1.9281288E-10 20.362717 2 ATAACGC 210 7.1227325E-5 19.842094 3 AAAAGTA 2085 0.0 19.41238 2 TGGGTAT 430 4.9112714E-11 19.379187 6 GGGCCTA 185 6.938959E-4 19.304363 7 >>END_MODULE