##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364134_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330146 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09162678382674 33.0 31.0 34.0 30.0 34.0 2 31.90055377379626 33.0 31.0 34.0 30.0 34.0 3 32.11949064238061 34.0 31.0 34.0 30.0 34.0 4 35.61551137995378 37.0 35.0 37.0 33.0 37.0 5 35.75633577065976 37.0 35.0 37.0 33.0 37.0 6 35.88927080185183 37.0 35.0 37.0 35.0 37.0 7 35.8685166891454 37.0 35.0 37.0 35.0 37.0 8 35.91517622877489 37.0 35.0 37.0 35.0 37.0 9 37.57424072244701 39.0 37.0 39.0 35.0 39.0 10-11 37.538160472609775 39.0 37.0 39.0 35.0 39.0 12-13 37.51427850777283 39.0 37.0 39.0 34.5 39.0 14-15 38.78520891691589 40.0 38.0 41.0 34.0 41.0 16-17 38.777171077460665 40.0 38.0 41.0 34.0 41.0 18-19 38.869451548927714 40.0 38.5 41.0 35.0 41.0 20-21 38.916882808353364 40.0 38.5 41.0 35.0 41.0 22-23 38.84230114588925 40.0 38.0 41.0 35.0 41.0 24-25 38.8002591444849 40.0 38.0 41.0 35.0 41.0 26-27 38.614548327777555 40.0 38.0 41.0 34.0 41.0 28-29 38.458177147471034 40.0 38.0 41.0 34.0 41.0 30-31 38.274716083798324 40.0 37.5 41.0 34.0 41.0 32-33 37.945803693729864 40.0 37.0 41.0 33.5 41.0 34-35 37.72259436182193 40.0 36.0 41.0 33.0 41.0 36-37 37.589429280695505 40.0 36.0 41.0 33.0 41.0 38-39 37.501567497101824 40.0 36.0 41.0 33.0 41.0 40-41 37.2969117675804 40.0 36.0 41.0 33.0 41.0 42-43 37.11025631772753 40.0 36.0 41.0 32.5 41.0 44-45 36.8473581095609 40.0 35.0 41.0 31.0 41.0 46-47 36.470308146624504 40.0 35.0 41.0 30.5 41.0 48-49 36.10203090487811 40.0 35.0 41.0 30.0 41.0 50-51 35.94553830932845 39.5 35.0 41.0 28.5 41.0 52-53 35.854891869012874 39.0 35.0 41.0 28.0 41.0 54-55 35.69153498939214 39.0 35.0 41.0 28.0 41.0 56-57 35.45783094487372 39.0 35.0 41.0 27.5 41.0 58-59 35.30282841131725 39.0 35.0 41.0 27.0 41.0 60-61 35.140500366125224 39.0 35.0 40.5 27.0 41.0 62-63 34.837432131510376 38.0 35.0 40.0 27.0 41.0 64-65 34.571418851765145 37.5 35.0 40.0 26.0 41.0 66-67 34.16793006181276 37.0 34.0 40.0 26.0 41.0 68-69 33.866927389925614 36.5 34.0 39.0 26.0 41.0 70-71 33.46400658273603 36.0 34.0 39.0 25.5 41.0 72-73 33.14884042804324 35.0 34.0 39.0 25.0 40.0 74-75 32.808016563595274 35.0 33.5 37.5 24.5 39.5 76-77 32.3462616133868 35.0 33.0 37.0 23.0 39.0 78-79 31.975844756891348 35.0 33.0 36.5 21.5 39.0 80-81 31.732120759676008 35.0 33.0 36.0 20.5 37.5 82-83 31.512122729384593 35.0 33.0 36.0 20.0 37.0 84-85 31.253930019712122 35.0 33.0 35.0 20.0 37.0 86-87 31.001116042900556 35.0 32.5 35.0 18.5 36.0 88-89 30.804926301323313 35.0 32.0 35.0 18.0 36.0 90-91 30.619972544367307 35.0 32.0 35.0 15.0 36.0 92-93 30.435528881791925 35.0 32.0 35.0 10.0 36.0 94-95 30.358621158880304 35.0 32.0 35.0 8.5 35.0 96-97 30.254673171215792 35.0 32.0 35.0 7.0 35.0 98-99 30.15599565761954 35.0 32.0 35.0 2.0 35.0 100-101 29.96438548851028 35.0 32.0 35.0 2.0 35.0 102-103 28.655713733680365 33.5 29.0 34.5 2.0 35.0 104-105 29.479428574006164 34.0 30.5 35.0 2.0 35.0 106-107 29.542915965615805 35.0 31.0 35.0 2.0 35.0 108-109 29.492811315449583 35.0 31.0 35.0 2.0 35.0 110-111 29.485069684079793 35.0 31.0 35.0 2.0 35.0 112-113 29.43138948656764 35.0 31.0 35.0 2.0 35.0 114-115 29.3167001968205 35.0 31.0 35.0 2.0 35.0 116-117 29.123510501854682 35.0 31.0 35.0 2.0 35.0 118-119 28.962773635375363 35.0 31.0 35.0 2.0 35.0 120-121 28.77374588954897 35.0 31.0 35.0 2.0 35.0 122-123 28.5043213301397 35.0 30.5 35.0 2.0 35.0 124-125 27.575040258738515 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27829681718489496 1101 2 0.5587622181998917 1101 3 0.4342870121017768 1101 4 0.4958399755542331 1101 5 0.26864148245842756 1101 6 0.2732744018229951 1101 7 0.21354918445533855 1101 8 0.1857831631393907 1101 9 0.2883550830804822 1101 10-11 0.2125885737695441 1101 12-13 0.20272764472318272 1101 14-15 0.2623293052752942 1101 16-17 0.28912578644781206 1101 18-19 0.2940901455417304 1101 20-21 0.21606657678164964 1101 22-23 0.2261672909392587 1101 24-25 0.30494025579077544 1101 26-27 0.23297806367126128 1101 28-29 0.2730080083237425 1101 30-31 0.26139314240872835 1101 32-33 0.3371054541841829 1101 34-35 0.41239063775372387 1101 36-37 0.4179174868050879 1101 38-39 0.4249129111804706 1101 40-41 0.4977475537806413 1101 42-43 0.5040958410146672 1101 44-45 0.46988699263936695 1101 46-47 0.4552711513752925 1101 48-49 0.5266338243714372 1101 50-51 0.5118624898352735 1101 52-53 0.40147561871385307 1101 54-55 0.4146493578925501 1101 56-57 0.5220865048588976 1101 58-59 0.4885182316210006 1101 60-61 0.416190413181873 1101 62-63 0.37316417039689753 1101 64-65 0.39445353996008947 1101 66-67 0.43579477698482094 1101 68-69 0.43412165100569666 1101 70-71 0.39278586571924734 1101 72-73 0.4470260461046678 1101 74-75 0.46554371219737334 1101 76-77 0.4318707971006148 1101 78-79 0.44654627044492656 1101 80-81 0.40203916459236666 1101 82-83 0.45121034341485 1101 84-85 0.4014209896119745 1101 86-87 0.388671065506486 1101 88-89 0.40248830569741756 1101 90-91 0.4723551097515646 1101 92-93 0.4813926197132936 1101 94-95 0.4345106351123995 1101 96-97 0.4909552610701482 1101 98-99 0.4959611566538591 1101 100-101 0.5128368258965423 1101 102-103 0.4965046521841323 1101 104-105 0.5772549273225138 1101 106-107 0.49453744903525276 1101 108-109 0.5238830226020497 1101 110-111 0.41514376909763584 1101 112-113 0.5094170479646358 1101 114-115 0.4927440443152449 1101 116-117 0.36403551585259564 1101 118-119 0.3728980162142328 1101 120-121 0.4606213129132719 1101 122-123 0.5364212482809769 1101 124-125 0.5535450739946555 1102 1 0.1728877423945292 1102 2 0.5964137144141013 1102 3 0.4425107336885574 1102 4 0.4438684438682472 1102 5 0.20496986901614633 1102 6 0.1728687383894112 1102 7 0.153981768805167 1102 8 0.14282913427683042 1102 9 0.2784381024509628 1102 10-11 0.12657138433645088 1102 12-13 0.19352596562747948 1102 14-15 0.3079898269195738 1102 16-17 0.3098322728877321 1102 18-19 0.2846325486272292 1102 20-21 0.17717882706690347 1102 22-23 0.1889792741821239 1102 24-25 0.1379228967146915 1102 26-27 0.16401977206604812 1102 28-29 0.23573120644825707 1102 30-31 0.212007345173852 1102 32-33 0.23716102415557572 1102 34-35 0.13399631133417955 1102 36-37 0.24389442698453223 1102 38-39 0.1450866604861787 1102 40-41 0.18576087577625344 1102 42-43 0.2467285258819345 1102 44-45 0.20436835806993514 1102 46-47 0.17492526992139545 1102 48-49 0.2383967691624207 1102 50-51 0.21211943819562862 1102 52-53 0.10888677149877424 1102 54-55 0.11662480035160883 1102 56-57 0.2752288638689251 1102 58-59 0.20165391060783833 1102 60-61 0.12530513042505476 1102 62-63 0.04907642427582459 1102 64-65 0.06563138025152426 1102 66-67 0.12591661342043636 1102 68-69 0.2032651494247375 1102 70-71 0.03843732388268961 1102 72-73 0.09165766449353896 1102 74-75 0.1562316858831565 1102 76-77 0.19073001931127465 1102 78-79 0.18407724614581156 1102 80-81 0.0669033524278575 1102 82-83 0.09639158396491965 1102 84-85 0.09244322869867005 1102 86-87 0.1237649425570666 1102 88-89 0.08080396744514218 1102 90-91 0.2058696158129969 1102 92-93 0.14302804159322235 1102 94-95 0.15457287453732604 1102 96-97 0.09409080265144354 1102 98-99 0.09928880175014854 1102 100-101 0.0642551681621164 1102 102-103 0.009289841346642902 1102 104-105 0.07390447819136625 1102 106-107 0.10403988771935246 1102 108-109 0.013413513155683177 1102 110-111 0.007542521032544158 1102 112-113 0.09515400936794904 1102 114-115 0.053578497591811214 1102 116-117 -0.07091987226785079 1102 118-119 -0.13760135251300554 1102 120-121 -0.0975099505993775 1102 122-123 -0.15743101645299262 1102 124-125 -0.08391793881206411 1103 1 0.12859028684596296 1103 2 0.537568162006405 1103 3 0.29709646243777144 1103 4 0.4404366572705598 1103 5 0.14347023142239834 1103 6 0.17623981998578842 1103 7 0.17514775295308027 1103 8 0.22171593482103447 1103 9 0.3063894988483682 1103 10-11 0.29193206844273334 1103 12-13 0.21487562799825355 1103 14-15 0.37037151078773434 1103 16-17 0.37532593481662957 1103 18-19 0.2573591984354451 1103 20-21 0.16643130620183655 1103 22-23 0.15581506376660315 1103 24-25 0.12648860167170284 1103 26-27 0.17679335491691717 1103 28-29 0.11068567769728332 1103 30-31 0.17895295088731444 1103 32-33 0.09605355129175308 1103 34-35 0.21132914200578767 1103 36-37 0.1926244939893067 1103 38-39 0.0924581599830816 1103 40-41 0.07989604314749954 1103 42-43 0.026943723808891207 1103 44-45 -0.12897778497397638 1103 46-47 -0.020547960736244875 1103 48-49 -0.08992649096190064 1103 50-51 -0.054354153565583374 1103 52-53 -0.01851648218507762 1103 54-55 -0.07948557228331055 1103 56-57 -0.14562366883230737 1103 58-59 -0.23998526511724805 1103 60-61 -0.1620409511322478 1103 62-63 -0.09804683605691622 1103 64-65 -0.1730957270587652 1103 66-67 -0.23321508621017273 1103 68-69 -0.2375371831063191 1103 70-71 -0.17685986748188043 1103 72-73 -0.09111089521386617 1103 74-75 -0.13457523209005728 1103 76-77 -0.16890555788386052 1103 78-79 -0.23383565338037826 1103 80-81 -0.32409078706442074 1103 82-83 -0.28395169369871454 1103 84-85 -0.30541109701466596 1103 86-87 -0.4377793770440803 1103 88-89 -0.3892390262858285 1103 90-91 -0.30859628178469833 1103 92-93 -0.3289246845824074 1103 94-95 -0.29163037935495595 1103 96-97 -0.2731786238608578 1103 98-99 -0.23674620810073677 1103 100-101 -0.26011653507432086 1103 102-103 -0.35327051844382495 1103 104-105 -0.2044249566476779 1103 106-107 -0.22662390192974158 1103 108-109 -0.022692935799749847 1103 110-111 0.009604276516967047 1103 112-113 -0.03172999509869001 1103 114-115 -0.12171878756589649 1103 116-117 -0.07933103219192006 1103 118-119 -0.19866641524758677 1103 120-121 -0.24188572341572723 1103 122-123 -0.177715345131233 1103 124-125 -0.06475248407429035 1104 1 0.18813731278107326 1104 2 0.5252779717380172 1104 3 0.02340225559517961 1104 4 0.34978195864508166 1104 5 0.12034828549914067 1104 6 0.1843017427191853 1104 7 0.07575291446297427 1104 8 0.11471367158174672 1104 9 0.17920333798335264 1104 10-11 0.13153274315435937 1104 12-13 0.11391658126090931 1104 14-15 0.21175122742150876 1104 16-17 0.2779526020902665 1104 18-19 0.23726415553078084 1104 20-21 0.16622450596745608 1104 22-23 0.041963197001940955 1104 24-25 0.07491768671881971 1104 26-27 0.10238434227301241 1104 28-29 0.24915431891871265 1104 30-31 0.18059396369481107 1104 32-33 0.21488347337468383 1104 34-35 0.28221244800514 1104 36-37 0.2616898080517913 1104 38-39 0.16745982144465188 1104 40-41 0.31534681288499655 1104 42-43 0.13936904628459246 1104 44-45 0.10541549186398669 1104 46-47 0.038392418719674026 1104 48-49 -0.03583307568097638 1104 50-51 0.03143593165948033 1104 52-53 -0.0049021131351523195 1104 54-55 0.02015501645590234 1104 56-57 0.04377698538187502 1104 58-59 0.049628543052470775 1104 60-61 0.09186117088889745 1104 62-63 0.11254299131905299 1104 64-65 0.07854899817200334 1104 66-67 0.03314325051708522 1104 68-69 0.17871091290741248 1104 70-71 0.19788930050928855 1104 72-73 0.17718033599366834 1104 74-75 0.144401570498367 1104 76-77 0.103930774884617 1104 78-79 0.07774292011914952 1104 80-81 0.0855384842611393 1104 82-83 0.05308485285665654 1104 84-85 0.08518646680797914 1104 86-87 0.1387735904723364 1104 88-89 0.032618967316143 1104 90-91 0.21335667682189907 1104 92-93 0.15203082744109508 1104 94-95 0.14270013927179193 1104 96-97 0.127252358051976 1104 98-99 0.10511697559070043 1104 100-101 0.14389224912774878 1104 102-103 -0.050583502991134566 1104 104-105 0.10351897188488834 1104 106-107 -0.029214394377213182 1104 108-109 -0.05964834460571211 1104 110-111 0.03890430336813111 1104 112-113 0.011830119015119323 1104 114-115 0.04029525927003874 1104 116-117 -0.02893385158873585 1104 118-119 0.05651411928780803 1104 120-121 0.20595472953399252 1104 122-123 0.25894523640709366 1104 124-125 0.2837496079066568 1105 1 0.09022184003391942 1105 2 0.4626394791568522 1105 3 0.18974324350350713 1105 4 0.42697581853043687 1105 5 0.12927576397611062 1105 6 0.03377020600674996 1105 7 0.1077977557064429 1105 8 0.1352504194873987 1105 9 0.1354895209267113 1105 10-11 0.06109969449641284 1105 12-13 0.07402097500127525 1105 14-15 0.222421682653966 1105 16-17 0.25693045876596443 1105 18-19 0.12364140694862158 1105 20-21 0.15352615711129403 1105 22-23 0.13210709688308242 1105 24-25 0.04295528525309322 1105 26-27 0.15193489559381135 1105 28-29 0.22510988337887028 1105 30-31 0.19292045918984968 1105 32-33 0.05642447767104386 1105 34-35 0.2613749198194739 1105 36-37 0.2065853118862364 1105 38-39 0.2379172273096657 1105 40-41 0.22244542734712525 1105 42-43 0.22793547350949694 1105 44-45 0.29550837900992377 1105 46-47 0.25298528363185113 1105 48-49 0.24884296846877163 1105 50-51 0.15507561287235205 1105 52-53 0.11636067939490857 1105 54-55 0.12920323173464254 1105 56-57 0.1368107741991409 1105 58-59 0.08186711777363342 1105 60-61 0.056299148945925026 1105 62-63 0.13352778692586043 1105 64-65 0.13172790098349907 1105 66-67 0.23133309783362677 1105 68-69 0.17099879660837303 1105 70-71 0.16334815781402767 1105 72-73 0.183108630374619 1105 74-75 0.17460925378572512 1105 76-77 -0.003073433541132431 1105 78-79 -3.847576122666396E-4 1105 80-81 -0.02751642912463126 1105 82-83 0.0033727925167319484 1105 84-85 0.06661340942639171 1105 86-87 0.193246298159373 1105 88-89 0.1446393864000015 1105 90-91 0.10510687829972198 1105 92-93 0.1426700404959611 1105 94-95 0.10604166828484551 1105 96-97 0.09782160721545807 1105 98-99 0.1998130501082649 1105 100-101 0.0616002384415566 1105 102-103 -0.08266831503593508 1105 104-105 0.11090157889046992 1105 106-107 0.0249507879570956 1105 108-109 0.012913274131797436 1105 110-111 0.06111782638956598 1105 112-113 0.01668332374906001 1105 114-115 0.06688683412460605 1105 116-117 0.10465306317003353 1105 118-119 0.10463759009238416 1105 120-121 0.1779875345699118 1105 122-123 0.06476708354775695 1105 124-125 0.07477039222371218 1106 1 0.2244556873808463 1106 2 0.4307563215276353 1106 3 0.35684734637015936 1106 4 0.39726490166555095 1106 5 0.19658083038372354 1106 6 0.05952595608648892 1106 7 0.06076624153789112 1106 8 0.026377795766613588 1106 9 0.16022273348734473 1106 10-11 0.11861847228785649 1106 12-13 0.11767582220436168 1106 14-15 0.18259301241136683 1106 16-17 0.17791572213451445 1106 18-19 0.1117235289068077 1106 20-21 0.05836111267625199 1106 22-23 0.013783558090246117 1106 24-25 0.0372351728469269 1106 26-27 0.08559138969727798 1106 28-29 0.16432622980332923 1106 30-31 -0.01230287301080324 1106 32-33 0.02932071877726372 1106 34-35 0.07641810404925309 1106 36-37 -0.04790275225531815 1106 38-39 0.05098621575842799 1106 40-41 0.05808607159788437 1106 42-43 0.09691099723424657 1106 44-45 0.1143777402001902 1106 46-47 0.2674337075064912 1106 48-49 0.15822045054569855 1106 50-51 0.04937509649612082 1106 52-53 0.04729978785893252 1106 54-55 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-0.747760964453775 2216 94-95 -0.7865541073114244 2216 96-97 -0.9726560008458733 2216 98-99 -0.9050424743391723 2216 100-101 -0.9837329531332628 2216 102-103 -1.0722918871142362 2216 104-105 -0.9710953851146797 2216 106-107 -1.054457606509601 2216 108-109 -0.9673158430670554 2216 110-111 -1.0271903448074653 2216 112-113 -1.128861324276361 2216 114-115 -1.1885605321203272 2216 116-117 -1.1136166560299756 2216 118-119 -1.2746934278518793 2216 120-121 -1.385794936989786 2216 122-123 -1.4553183259571512 2216 124-125 -1.5757239905951508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32.0 3 14.0 4 43.0 5 216.0 6 648.0 7 1042.0 8 1715.0 9 3132.0 10 6239.0 11 8001.0 12 7815.0 13 10247.0 14 14578.0 15 14630.0 16 8031.0 17 6601.0 18 6597.0 19 6458.0 20 6718.0 21 6442.0 22 7080.0 23 7507.0 24 8542.0 25 9748.0 26 11733.0 27 14036.0 28 16976.0 29 21026.0 30 26502.0 31 34134.0 32 43942.0 33 58607.0 34 84370.0 35 137382.0 36 203343.0 37 353094.0 38 181628.0 39 1297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.48973943526237 18.79208728858478 12.366811424854237 22.351361851298606 2 13.507860803040876 23.386189765920083 38.15737243486419 24.94857699617485 3 20.988692919542302 29.559895950802172 29.5690679176628 19.882343211992723 4 12.662594933187785 19.14135741490032 35.062617186384045 33.133430465527844 5 10.895570862145961 39.39469802563027 35.35168319868646 14.35804791353731 6 29.673678180104023 33.238302741602496 19.53743258015575 17.550586498137733 7 22.6586404800676 33.99175729581565 24.593240140556 18.756362083560752 8 26.724735480165336 32.68445719492446 23.169035579552922 17.421771745357276 9 28.187807954916227 14.599600344623823 21.997134149183623 35.21545755127632 10-11 23.047842940313227 27.813830682993725 27.271896877330576 21.866429499362475 12-13 26.530956711203267 25.976623733992348 27.761872146290372 19.730547408514006 14-15 26.000433042332894 24.208870751899823 27.806091762873237 21.984604442894046 16-17 20.733351652213923 27.82741960523689 32.005853521378775 19.433375221170408 18-19 22.069005958744377 25.13592492854168 34.54233595409828 18.252733158615673 20-21 20.457686771860672 24.98749196986345 35.13465256244523 19.420168695830643 22-23 19.40388498706157 26.14066425790853 31.91871418626226 22.53673656876764 24-25 20.142187398977253 27.183933192296184 31.01366316178826 21.660216246938305 26-27 21.35427238814386 24.24211327177618 32.05193264498784 22.351681695092118 28-29 20.931815704302913 23.484018659619814 34.496201541937154 21.087964094140112 30-31 22.566695686037473 25.066458869928564 33.12820547518844 19.238639968845526 32-33 18.714035902825703 25.35808099261284 34.528427706432225 21.399455398129227 34-35 17.501424655639305 26.27422102536113 35.37908620557443 20.845268113425142 36-37 21.826100292749818 24.978686550198248 32.65934716940847 20.53586598764346 38-39 22.846777722144786 23.91936674620681 32.517445453356245 20.716410078292157 40-41 20.314236181592097 25.796942591264415 32.56586119117751 21.322960035965977 42-43 22.503469543944803 25.13318086886703 31.923788817167438 20.439560770020734 44-45 23.798289811795094 23.501405108912856 31.77918063129912 20.921124447992927 46-47 21.051974745629426 25.22365965841344 34.75780102334631 18.966564572610825 48-49 22.573762579446168 25.114987377325498 33.22714198291015 19.08410806031819 50-51 20.133466551792058 25.8154368016744 33.150082772868544 20.901013873664997 52-53 19.4204245248266 26.99147311648496 29.93584914738683 23.652253211301616 54-55 20.436102503033503 27.63335754120224 28.95193459966049 22.978605356103763 56-57 21.834332471773777 24.95372688411648 29.725797017770983 23.486143626338762 58-59 21.419002124578807 24.098332062796114 32.14868894091325 22.333976871711826 60-61 22.898726906670397 25.709546170119673 30.940062218733882 20.45166470447605 62-63 19.270328219608977 25.949482237288237 32.435499561702244 22.34468998140054 64-65 18.009564363611215 26.603846495046408 33.432194661337924 21.95439448000445 66-67 21.960596806666334 25.70424600006315 30.840411503699595 21.49474568957092 68-69 22.99240083419414 24.646542559989655 30.677497056714074 21.68355954910213 70-71 20.688142504657385 26.173217075418787 30.852703387447693 22.285937032476134 72-73 22.60421036487724 25.65759698559406 30.264083792305506 21.474108857223193 74-75 23.673115582800687 24.065102627831834 30.259385059985895 22.002396729381587 76-77 20.85316198372209 25.570238154307873 33.68430984267892 19.892290019291114 78-79 22.199066869351185 25.297223011609255 32.51597944887253 19.987730670167032 80-81 19.803991441541005 26.061124117202173 32.39392517813834 21.740959263118484 82-83 19.282808052649862 26.833370171394723 29.403238441494395 24.480583334461027 84-85 20.13083526169308 27.269863608957213 28.668243937131717 23.931057192218 86-87 21.280145187069692 24.728338092209427 29.60614849798443 24.38536822273645 88-89 21.02686472011343 24.110022508807305 31.57269953824618 23.29041323283309 90-91 22.297627478487325 25.542120940107328 30.373131971979017 21.787119609426334 92-93 19.06426061500016 25.691615807588043 31.487520918756285 23.756602658655517 94-95 17.8640003728942 26.354974228199225 32.52693709515418 23.2540883037524 96-97 21.512449435972155 25.35980762645189 30.367436634500404 22.76030630307555 98-99 22.29227963772635 24.317391292907033 30.17338176580023 23.21694730356639 100-101 20.095914283093734 25.787131638181073 30.332196616010577 23.78475746271462 102-103 21.97414925377623 24.994116970416442 30.042884090408446 22.988849685398886 104-105 22.753141772175695 23.578151876603783 30.208799781932345 23.459906569288176 106-107 20.240936858873017 24.96970713197314 33.10894868051673 21.68040732863711 108-109 21.650840342295254 24.92032322621662 31.723255316453177 21.705581115034953 110-111 19.28132906752484 25.39352835246305 32.016308822478955 23.308833757533147 112-113 18.604965915909368 26.193995846447898 29.339424307453083 25.861613930189648 114-115 19.356509103817896 26.783102074522773 28.57272863334805 25.287660188311285 116-117 20.480367326447844 24.476772280541436 29.226030990348654 25.816829402662062 118-119 19.999947362128253 23.894736288022404 30.973815666684967 25.131500683164376 120-121 21.103262115920344 25.16186516186516 30.024737493091923 23.71013522912257 122-123 18.359564863220008 25.478747818320358 30.843452480075907 25.31823483838373 124-125 17.240299552145334 26.127699852419216 31.5812344609768 25.050766134458648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4671.0 1 3337.0 2 5416.5 3 6975.0 4 3296.5 5 1621.5 6 2691.5 7 6100.0 8 9425.5 9 9607.5 10 6715.0 11 3420.5 12 2271.5 13 2219.0 14 2472.5 15 2823.5 16 2975.0 17 3200.0 18 3505.0 19 3451.5 20 3409.5 21 3804.5 22 4434.5 23 5083.5 24 5776.5 25 6653.0 26 7866.0 27 9198.0 28 10147.0 29 11475.0 30 13760.5 31 15694.0 32 17861.0 33 20562.0 34 22824.0 35 24972.5 36 27800.0 37 30617.0 38 31481.0 39 31508.5 40 30641.0 41 29750.5 42 29517.5 43 29974.0 44 31261.0 45 32860.5 46 34549.5 47 38255.0 48 44209.0 49 66703.5 50 150498.5 51 150132.5 52 60870.0 53 31798.5 54 25675.5 55 22686.5 56 20107.0 57 17617.0 58 16417.0 59 15295.5 60 13392.5 61 11490.0 62 9477.5 63 7053.5 64 5207.0 65 4006.5 66 2771.0 67 2010.0 68 1551.0 69 1051.5 70 807.0 71 728.0 72 675.5 73 675.0 74 513.0 75 383.5 76 289.0 77 178.5 78 126.0 79 66.0 80 33.5 81 22.5 82 10.0 83 3.0 84 3.5 85 5.5 86 4.5 87 1.0 88 0.0 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1469011672402879 2 1.5035943422752089E-4 3 4.510783026825627E-4 4 0.0 5 0.0 6 0.0036086264214605017 7 0.0 8 0.0 9 0.0 10-11 1.5035943422752089E-4 12-13 0.005939197651987075 14-15 0.0016539537765027298 16-17 4.886681612394428E-4 18-19 0.0 20-21 3.758985855688022E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 7.517971711376044E-5 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.517971711376044E-4 96-97 0.0019170827864008913 98-99 0.0034958568457898603 100-101 0.0 102-103 0.0036838061385742614 104-105 0.021313449801751084 106-107 0.015975689886674093 108-109 0.01815590168297315 110-111 0.014359325968728244 112-113 0.015035943422752088 114-115 0.04052186752431688 116-117 0.040484277665759995 118-119 0.022929813719696934 120-121 0.01360752879759064 122-123 0.0026688799575384954 124-125 0.002029852362071532 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1330146.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.47053081426834 #Duplication Level Percentage of deduplicated Percentage of total 1 83.85170724640814 38.96633345423218 2 10.348382868456234 9.61789689932884 3 2.599186904223465 3.6235678537427782 4 1.0079601515824466 1.8736177313466673 5 0.534513744303712 1.24195687126578 6 0.3107128886405076 0.8663395719575423 7 0.20143178405891649 0.6552449349658049 8 0.14385474894523734 0.5348005234910791 9 0.11016263112374065 0.4607384349795 >10 0.7486236884045897 6.921478442180592 >50 0.08330861761979674 2.6622078171852848 >100 0.05063383431303031 4.502338945570622 >500 0.002596606887341297 0.8266606022898446 >1k 0.0036785264237335037 5.330708978128152 >5k 0.0012983034436706484 4.375841069194401 >10k+ 0.0019474551655059725 17.540267870140987 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 42508 3.195739415071729 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 37164 2.7939790068157935 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 22704 1.706880297350817 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13192 0.9917708281647277 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12884 0.9686154752936895 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 12640 0.950271624317932 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 11861 0.8917066246863127 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11171 0.8398326198778179 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10311 0.7751780631599839 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9825 0.7386407206426964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8642 0.6497031152971178 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8017 0.6027157921010176 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6056 0.45528836684093327 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 5879 0.44198155691179764 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5098 0.3832661978459508 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 4883 0.3671025586664922 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4830 0.36311803365946294 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4449 0.3344745614391202 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4086 0.30718432412682517 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4052 0.30462821374495735 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4007 0.3012451264748381 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3906 0.29365197504634827 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3899 0.29312571702655194 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3374 0.25365636554182774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3182 0.23922185985598574 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2479 0.18637051872501215 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2313 0.1738906856841279 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2232 0.1678011285979133 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1439 0.10818361292670127 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1400 0.10525160395926463 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.5035943422752089E-4 0.0 0.0 0.0 0.0 3 1.5035943422752089E-4 0.0 0.0 0.0 0.0 4 1.5035943422752089E-4 0.0 0.0 0.0 0.0 5 1.5035943422752089E-4 0.0 0.0 0.0 0.0 6 2.2553915134128133E-4 0.0 0.0 0.0 0.0 7 3.0071886845504177E-4 0.0 0.0 0.0 0.0 8 3.0071886845504177E-4 0.0 0.0 0.0 0.0 9 3.0071886845504177E-4 0.0 0.0 1.5035943422752089E-4 0.0 10-11 3.758985855688022E-4 0.0 0.0 3.3830872701192196E-4 0.0 12-13 3.758985855688022E-4 0.0 0.0 6.014377369100835E-4 0.0 14-15 3.758985855688022E-4 0.0 0.0 0.0011652856152632868 0.0 16-17 3.758985855688022E-4 0.0 0.0 0.002518520523310975 0.0 18-19 3.758985855688022E-4 0.0 0.0 0.003683806138574262 0.0 20-21 3.758985855688022E-4 0.0 0.0 0.005112220763735711 0.0 22-23 3.758985855688022E-4 0.0 0.0 0.008833616760866851 0.0 24-25 4.5107830268256266E-4 0.0 0.0 0.012780551909339275 0.0 26-27 4.5107830268256266E-4 0.0 0.0 0.017404104511835543 0.0 28-29 4.5107830268256266E-4 0.0 0.0 0.022478735417014374 0.0 30-31 4.5107830268256266E-4 0.0 0.0 0.031763430480563785 0.0 32-33 4.5107830268256266E-4 0.0 0.0 0.049505843719411254 0.0 34-35 4.5107830268256266E-4 0.0 0.0 0.07277396616612011 0.0 36-37 5.262580197963232E-4 0.0 0.0 0.10600340113040223 0.0 38-39 5.262580197963232E-4 0.0 0.0 0.16332793542964458 0.0 40-41 5.262580197963232E-4 0.0 0.0 0.24425890090260768 0.0 42-43 5.262580197963232E-4 0.0 0.0 0.3088382779033279 0.0 44-45 5.262580197963232E-4 0.0 0.0 0.37646243344715546 0.0 46-47 5.262580197963232E-4 0.0 0.0 0.47081297842492476 0.0 48-49 5.262580197963232E-4 0.0 0.0 0.5605775606587547 0.0 50-51 5.262580197963232E-4 0.0 0.0 0.654364257758171 0.0 52-53 5.262580197963232E-4 0.0 0.0 0.7708928192844996 0.0 54-55 5.262580197963232E-4 0.0 0.0 0.8893760534557861 0.0 56-57 6.014377369100835E-4 0.0 0.0 1.0224065628885852 0.0 58-59 6.76617454023844E-4 0.0 0.0 1.1805095079788233 0.0 60-61 6.76617454023844E-4 0.0 0.0 1.3285007811172609 0.0 62-63 6.76617454023844E-4 0.0 0.0 1.4802510401113862 0.0 64-65 6.76617454023844E-4 0.0 0.0 1.6594418958520343 0.0 66-67 6.76617454023844E-4 0.0 0.0 1.8627654407861993 0.0 68-69 8.269768882513649E-4 0.0 0.0 2.108565525889639 0.0 70-71 8.645667468082451E-4 0.0 0.0 2.441874801713496 0.0 72-73 9.021566053651253E-4 0.0 0.0 2.721656118952356 0.0 74-75 9.021566053651253E-4 0.0 0.0 2.9888448335746602 0.0 76-77 9.021566053651253E-4 0.0 0.0 3.301103788606664 0.0 78-79 9.021566053651253E-4 0.0 0.0 3.6094533983487525 0.0 80-81 9.021566053651253E-4 0.0 0.0 3.948100434087687 0.0 82-83 9.773363224788857E-4 0.0 0.0 4.362077546374609 0.0 84-85 0.001014926181035766 0.0 0.0 4.760191738350526 0.0 86-87 0.0010525160395926463 0.0 0.0 5.170973712660114 0.0 88-89 0.0010525160395926463 0.0 0.0 5.630584913235088 0.0 90-91 0.0010525160395926463 0.0 0.0 6.101736200387025 0.0 92-93 0.0010525160395926463 0.0 0.0 6.578413196746823 0.0 94-95 0.0010525160395926463 0.0 0.0 7.0921913835022625 0.0 96-97 0.0010525160395926463 0.0 0.0 7.612359846212371 0.0 98-99 0.0011276957567064067 0.0 0.0 8.203046883575187 0.0 100-101 0.0011276957567064067 0.0 0.0 8.990592010200384 0.0 102-103 0.0011276957567064067 0.0 0.0 9.661232676713684 0.0 104-105 0.0011276957567064067 0.0 0.0 10.304395156621904 0.0 106-107 0.0011276957567064067 0.0 0.0 10.993191724818178 0.0 108-109 0.0011276957567064067 0.0 0.0 11.6360910757165 0.0 110-111 0.001202875473820167 0.0 0.0 12.29733427759058 0.0 112-113 0.001202875473820167 0.0 0.0 13.100366425941212 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1235 0.0 42.394657 5 AAAGTAC 1345 0.0 36.715652 3 AAGTACT 1570 0.0 32.969704 4 ACGTTTA 110 7.74902E-4 27.047163 7 TATAACG 90 0.009531794 26.443132 2 AAAAGTA 1910 0.0 26.166243 2 GTTATCA 880 0.0 25.737381 1 AAAAAGT 2030 0.0 24.075829 1 CGGTTAC 180 3.6379788E-12 23.136871 28-29 ATATAGG 495 0.0 22.837254 2 GAGTACT 12940 0.0 21.795767 12-13 ACTTGAC 475 0.0 21.296083 8 GGCTTAC 510 0.0 21.001328 7 TATAGGC 550 0.0 20.553526 3 GTGTTAT 495 0.0 20.469498 1 GATATAG 620 0.0 20.187895 1 AGTACTT 13145 0.0 20.075258 12-13 ACACCGT 180 5.780359E-4 19.835333 6 AGGCTTA 530 0.0 19.086828 6 GTACTTT 14905 0.0 18.841064 14-15 >>END_MODULE