FastQCFastQC Report
Thu 26 May 2016
SRR1364133_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364133_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353263
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA651721.9435397700687358No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT503811.5024470195150217No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG326740.9743941945502038No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC200040.5965532676679401No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC154270.4600593511454366No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG153900.45895594828082376No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT146730.437573790066571No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125130.37315892013242025No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG122070.36403348022508225No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT104110.31047370874279767No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC76240.22736063350831714No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT71920.21447765952148698No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA64730.19303585790914699No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG64560.192528889025406No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC57630.17186245158819932No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG55920.16676294105174572No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG45840.13670266841580872No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41810.12468452370124265No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC41670.1242670199146324No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40260.12006216034948645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG102800.026.810614
ATGGGGG43600.023.7571645
GTATAGG11800.023.2029191
ATGGGAG52200.023.03635
ATGGGAT56250.021.4835155
AGTACTC18400.021.0294235
TATACCG2556.362534E-721.0103915
ATGGGGA57200.020.1900565
CATGGGT55600.018.3079684
CGCAAAA7250.017.240172
ACCGTCC4753.790774E-916.2903188
GTGTAGC15800.016.1986261
AAGTACT26200.016.1315424
GTACTAG7500.015.8721411
GTACATA20650.015.5646911
GTCTATG28950.015.2142281
GGGCGTA7450.015.1801879
ATGGGTA33450.015.1270645
TACACTG24450.015.0954025
TACATGG1156200.015.0008842
Produced by FastQC (version 0.11.5)