FastQCFastQC Report
Thu 26 May 2016
SRR1364133_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364133_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353263
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA610321.8200779360282804No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG338461.0093452258292894No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC179240.5345241336572765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173640.5178239821928671No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG164710.49119320494694274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158920.4739264412007051No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG143730.4286272803534945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113040.337104485988722No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT103180.307700290731744No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT85310.2544089145408517No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC83270.24832528793595968No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC73710.2198157436502893No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA73440.2190105577761124No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG70890.21140602451999738No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG63850.19041154839331123No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60240.17964591503857585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50850.15164333963664645No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG50800.15149423114142851No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA49150.14657365079923643No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42340.12626507375055282No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC36480.10878955811101007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCA20150.026.3196491
GTCTACG2351.0257281E-825.3569641
CATGGGG81700.023.5211544
AGTACTC19300.022.503175
GAGTACT203900.022.46968512-13
GCGCGAC1954.0391587E-521.3608889
GTACTTT221800.020.64135614-15
AGTACTT208700.020.5273812-13
CGCGGAA6100.019.99411682-83
ATGGGGG37650.019.594535
GTACCGT2158.5644315E-519.3732516
GTATAGG12400.019.2222141
TTATCAA26950.018.3230382
ACCGTCC4551.09139364E-1018.3087868
GTGTAGC15050.018.213211
ACTTTTT257100.018.0497316-17
ATTCGCG6550.017.71215680-81
CGATCGT6950.017.54905324-25
TCGCGGA6850.017.3707182-83
ATGGGAG40500.017.3342535
Produced by FastQC (version 0.11.5)