FastQCFastQC Report
Thu 26 May 2016
SRR1364132_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364132_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83590.4089318264317017No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.056.024641
GTATCAA34100.045.37211
TCAACGC37650.038.8927044
ATCAACG38050.038.6345833
CAACGCA39150.037.4053235
TATCAAC41350.036.410412
AACGCAG41900.034.950326
GTACATG67250.032.5630761
TACATGG65450.031.9137652
ACGCAGA48500.029.8274387
ACATGGG66950.029.6024483
CGCAGAG48100.029.3328788
TAAGGTG14450.026.7778425
GTAAGGT14750.025.4241544
GAGTACA46450.023.4441911
TGGTATC13450.023.0070882
CATGGGG44950.022.9094374
AGGTAAG14650.022.747362
GTGTAAG13450.022.1216561
GTGGTAT14850.022.039721
Produced by FastQC (version 0.11.5)