FastQCFastQC Report
Thu 26 May 2016
SRR1364132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2044106
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42590.20835514400916588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42370.20727887888397178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20870.10209842346727617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32100.073.138661
GTATCAA59100.052.0257071
ATCAACG74100.040.9474953
TCAACGC74350.040.6507644
CAACGCA74600.040.4347845
TATCAAC78100.039.003622
TGGTATC14950.038.6025962
AACGCAG78550.038.5529486
GTGGTAT16450.037.6723981
ACGCAGA92000.032.727477
CGCAGAG91500.032.7112128
GTACATG60200.028.0121041
TACATGG60800.027.399362
GCAGAGT107200.027.3098969
ACATGGG60400.027.2846373
TAAGGTG16300.026.6453385
GTAAGGT16450.026.0406954
GAGTACT78200.025.22411212-13
CAGAGTA101400.025.2040110-11
AGGTAAG15850.025.1496282
Produced by FastQC (version 0.11.5)