FastQCFastQC Report
Thu 26 May 2016
SRR1364131_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364131_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867862
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76850.4114329645337825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.059.1509741
GTATCAA25300.041.1641851
ATCAACG29000.035.0970153
CAACGCA29000.034.8983465
TCAACGC30150.033.960314
GTACATG59000.032.075771
AACGCAG31250.032.007246
TACATGG57500.031.9819052
TATCAAC32650.031.71612
ACATGGG56000.031.2486633
CATGGGG37700.027.3171444
ACGCAGA36850.026.4969187
CGCAGAG38350.025.1500388
TAGCCCT17200.024.573134
TATACCG1751.7441256E-523.812995
GAGTACA45700.022.919151
GTAGCCC14050.021.605583
AGTACAT45300.021.5456372
GTGTAGC14800.020.5073911
CAGAGTA43200.020.12166610-11
Produced by FastQC (version 0.11.5)