##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364131_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1867862 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.099908879778056 31.0 31.0 34.0 28.0 34.0 2 31.161767839379998 33.0 31.0 34.0 28.0 34.0 3 31.239499491932488 33.0 31.0 34.0 28.0 34.0 4 34.70674118323517 37.0 35.0 37.0 32.0 37.0 5 34.756929045079346 37.0 35.0 37.0 32.0 37.0 6 34.80123585147083 37.0 35.0 37.0 32.0 37.0 7 34.709348442229675 37.0 35.0 37.0 32.0 37.0 8 34.67244046937086 37.0 35.0 37.0 32.0 37.0 9 36.20610141434432 39.0 37.0 39.0 32.0 39.0 10-11 36.11208643893393 39.0 37.0 39.0 31.0 39.0 12-13 36.01497594576044 39.0 37.0 39.0 31.0 39.0 14-15 37.290267161064364 40.0 38.0 41.0 31.0 41.0 16-17 37.28953236373994 40.0 38.0 41.0 31.0 41.0 18-19 37.28728835427885 40.0 38.0 41.0 31.0 41.0 20-21 37.30642333320127 40.0 38.0 41.0 31.0 41.0 22-23 37.266482213354095 40.0 38.0 41.0 31.0 41.0 24-25 37.274932516427874 40.0 37.5 41.0 31.0 41.0 26-27 37.238433299676316 40.0 37.0 41.0 31.0 41.0 28-29 37.19301292065474 40.0 37.0 41.0 31.0 41.0 30-31 37.14469189908034 40.0 37.0 41.0 31.0 41.0 32-33 37.04604622825455 40.0 37.0 41.0 30.0 41.0 34-35 37.02337619160302 40.0 37.0 41.0 30.0 41.0 36-37 36.96359447325338 40.0 37.0 41.0 30.0 41.0 38-39 36.872130275148805 40.0 37.0 41.0 30.0 41.0 40-41 36.797201024486824 40.0 37.0 41.0 30.0 41.0 42-43 36.6808158204407 40.0 36.0 41.0 30.0 41.0 44-45 36.586331324262716 40.0 36.0 41.0 30.0 41.0 46-47 36.47681788055006 40.0 36.0 41.0 30.0 41.0 48-49 36.364054196723316 40.0 36.0 41.0 30.0 41.0 50-51 36.23366206925351 40.0 35.0 41.0 29.0 41.0 52-53 36.0881510518443 39.5 35.0 41.0 29.0 41.0 54-55 35.91104294642752 39.0 35.0 41.0 28.5 41.0 56-57 35.72210420255887 39.0 35.0 41.0 28.0 41.0 58-59 35.51035569008846 39.0 35.0 41.0 28.0 41.0 60-61 35.27026702186778 38.0 35.0 40.5 28.0 41.0 62-63 35.00576755670387 38.0 34.0 40.0 28.0 41.0 64-65 34.71632192313993 37.0 34.0 40.0 27.0 41.0 66-67 34.41079346332866 37.0 34.0 40.0 26.5 41.0 68-69 34.090139956806226 36.0 34.0 39.0 26.0 41.0 70-71 33.74676314417232 36.0 33.5 39.0 26.0 41.0 72-73 33.40239937425784 35.0 33.0 39.0 26.0 40.5 74-75 33.13315089658658 35.0 33.0 37.5 26.0 40.0 76-77 32.91173571709259 35.0 33.0 37.0 26.0 39.0 78-79 32.614190716444796 35.0 33.0 37.0 26.0 39.0 80-81 32.418578835053125 35.0 33.0 36.0 26.0 39.0 82-83 32.321884057815836 35.0 33.0 36.0 26.0 37.0 84-85 32.115766582327815 35.0 33.0 36.0 26.0 37.0 86-87 31.932272030803134 35.0 33.0 35.0 25.5 37.0 88-89 31.75826131694954 35.0 33.0 35.0 25.0 36.0 90-91 31.620771234705778 35.0 33.0 35.0 25.0 36.0 92-93 31.510754541823754 35.0 33.0 35.0 25.0 36.0 94-95 31.411520230081237 35.0 33.0 35.0 24.5 36.0 96-97 31.33545010284486 35.0 33.0 35.0 24.0 35.0 98-99 31.266044279502445 35.0 33.0 35.0 24.0 35.0 100-101 31.19877512364404 35.0 33.0 35.0 24.0 35.0 102-103 30.113640622272953 34.0 30.5 35.0 19.5 35.0 104-105 30.946916581631832 35.0 32.5 35.0 23.0 35.0 106-107 31.055087581416615 35.0 33.0 35.0 23.0 35.0 108-109 31.041735149598846 35.0 33.0 35.0 23.0 35.0 110-111 30.984605661446082 35.0 33.0 35.0 22.5 35.0 112-113 30.92067481430641 35.0 33.0 35.0 20.0 35.0 114-115 30.833824715102082 35.0 33.0 35.0 19.0 35.0 116-117 30.75268006951263 35.0 33.0 35.0 19.0 35.0 118-119 30.636022629080735 35.0 33.0 35.0 17.5 35.0 120-121 30.516011086472126 35.0 33.0 35.0 7.5 35.0 122-123 30.36957414412842 35.0 32.0 35.0 2.0 35.0 124-125 29.568958788176 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5784449412179988 1101 2 0.5997074891564189 1101 3 0.5482101293652697 1101 4 0.5930190338266783 1101 5 0.618307563028786 1101 6 0.603530042732956 1101 7 0.5960906207372219 1101 8 0.6473098967103112 1101 9 0.5043284831159625 1101 10-11 0.5753419082069158 1101 12-13 0.6189386023556551 1101 14-15 0.7828421897542626 1101 16-17 0.7348808160820397 1101 18-19 0.7910020888178266 1101 20-21 0.8117036323496478 1101 22-23 0.8683213644182857 1101 24-25 0.7667634993730843 1101 26-27 0.7229930815739181 1101 28-29 0.7278471333694796 1101 30-31 0.7975559571089406 1101 32-33 0.7600703221385814 1101 34-35 0.7773816997033833 1101 36-37 0.7895135222294272 1101 38-39 0.7636958145772326 1101 40-41 0.7915097227626688 1101 42-43 0.8588186300179004 1101 44-45 0.850015119629397 1101 46-47 0.8000112131167398 1101 48-49 0.7065271231778709 1101 50-51 0.8288443093908455 1101 52-53 0.8053758687760677 1101 54-55 0.7817633877669863 1101 56-57 0.8046932621734086 1101 58-59 0.7115858850288106 1101 60-61 0.7227627470528191 1101 62-63 0.7655811937549188 1101 64-65 0.7348220744938914 1101 66-67 0.7983109008585956 1101 68-69 0.7239358879260465 1101 70-71 0.691040995767203 1101 72-73 0.7169249031737266 1101 74-75 0.7232885468675576 1101 76-77 0.6848730873877926 1101 78-79 0.7359146198467954 1101 80-81 0.637224159782015 1101 82-83 0.6077866664069163 1101 84-85 0.614144147088183 1101 86-87 0.6057720857490914 1101 88-89 0.6136380590106825 1101 90-91 0.7138616195623406 1101 92-93 0.7293856072056784 1101 94-95 0.7178084684173207 1101 96-97 0.6811414592795728 1101 98-99 0.726477485858311 1101 100-101 0.6317700259155856 1101 102-103 0.7401137358956369 1101 104-105 0.6946261903809727 1101 106-107 0.6803047026155546 1101 108-109 0.6722199178427566 1101 110-111 0.6322988236372353 1101 112-113 0.7273858487876517 1101 114-115 0.7680001290223792 1101 116-117 0.7052560009804338 1101 118-119 0.7247469807961195 1101 120-121 0.7131580416784082 1101 122-123 0.7295215339771808 1101 124-125 0.7904772672273168 1102 1 0.3145392688829851 1102 2 0.3046544012382668 1102 3 0.29374173505346945 1102 4 0.32522803134445866 1102 5 0.3303317720217649 1102 6 0.33618641211744915 1102 7 0.39999114892587073 1102 8 0.3015099687182783 1102 9 0.3818552047635748 1102 10-11 0.34244710852050986 1102 12-13 0.3222451719296018 1102 14-15 0.4935666259491427 1102 16-17 0.49163521762261553 1102 18-19 0.505475186205139 1102 20-21 0.45002036482618024 1102 22-23 0.4060111209761601 1102 24-25 0.46860407544691185 1102 26-27 0.49248938999990344 1102 28-29 0.5092572066464669 1102 30-31 0.44042082320675746 1102 32-33 0.44166984164859713 1102 34-35 0.5631429999701822 1102 36-37 0.47533188446277563 1102 38-39 0.5224032148514297 1102 40-41 0.5036893777268006 1102 42-43 0.5190416505643682 1102 44-45 0.49940411677668095 1102 46-47 0.47928622621338945 1102 48-49 0.5388092376412956 1102 50-51 0.4986158838361092 1102 52-53 0.4202131394649058 1102 54-55 0.35776348757501353 1102 56-57 0.4165740349245013 1102 58-59 0.4589733908525204 1102 60-61 0.4610563800186043 1102 62-63 0.4873208704600671 1102 64-65 0.5406573284807621 1102 66-67 0.5101966136032559 1102 68-69 0.5265938565074393 1102 70-71 0.5350275554561321 1102 72-73 0.38679206397417687 1102 74-75 0.415661852100051 1102 76-77 0.5043741656800123 1102 78-79 0.47276765239164575 1102 80-81 0.4701729843470943 1102 82-83 0.4549561194021834 1102 84-85 0.5426735299052936 1102 86-87 0.5532634196519517 1102 88-89 0.564639686814175 1102 90-91 0.4479241228309938 1102 92-93 0.5183744119452456 1102 94-95 0.5498170559408386 1102 96-97 0.6023710564445253 1102 98-99 0.5203333814570925 1102 100-101 0.5409546081452419 1102 102-103 0.48928675954849865 1102 104-105 0.5029577793840936 1102 106-107 0.5686940542577794 1102 108-109 0.5967526316269627 1102 110-111 0.6379590859130886 1102 112-113 0.5941186214737222 1102 114-115 0.6200178328503583 1102 116-117 0.598106091204631 1102 118-119 0.6215024383863366 1102 120-121 0.6912177911526847 1102 122-123 0.6474388150450459 1102 124-125 0.7512181805691185 1103 1 0.27295685805450987 1103 2 0.2956142302324345 1103 3 0.33830861345455787 1103 4 0.2993750842246925 1103 5 0.28279475830992595 1103 6 0.3409576350015513 1103 7 0.3424437604641142 1103 8 0.38406599204500935 1103 9 0.3494546090063153 1103 10-11 0.4663341470555693 1103 12-13 0.4162192950953809 1103 14-15 0.44299433615080375 1103 16-17 0.42492938471483654 1103 18-19 0.48135315022341274 1103 20-21 0.45142912287114 1103 22-23 0.46044040320744983 1103 24-25 0.49673830547196474 1103 26-27 0.5586443346625174 1103 28-29 0.5472595863184111 1103 30-31 0.5258017224335276 1103 32-33 0.2861428501904939 1103 34-35 0.47795113165655323 1103 36-37 0.6045276702613194 1103 38-39 0.5528174365870129 1103 40-41 0.611055772962807 1103 42-43 0.6950907624483378 1103 44-45 0.6833313889405233 1103 46-47 0.7334442284252134 1103 48-49 0.6975884904656269 1103 50-51 0.7454362198953746 1103 52-53 0.637369825303665 1103 54-55 0.7060127888772456 1103 56-57 0.733962102864588 1103 58-59 0.7532834828953412 1103 60-61 0.7661320020678204 1103 62-63 0.6935458495265721 1103 64-65 0.7099105595342508 1103 66-67 0.792834498677891 1103 68-69 0.7835274109793886 1103 70-71 0.8271218281680817 1103 72-73 0.8186005178136355 1103 74-75 0.7775941379731535 1103 76-77 0.7451887675433326 1103 78-79 0.8188364485397557 1103 80-81 0.7923563559535935 1103 82-83 0.6447065508226331 1103 84-85 0.6349752330557479 1103 86-87 0.6303740227496526 1103 88-89 0.684178045950965 1103 90-91 0.730717596874424 1103 92-93 0.7456279180232244 1103 94-95 0.8083940431057179 1103 96-97 0.7280984994334361 1103 98-99 0.7996029913161422 1103 100-101 0.7721471620832894 1103 102-103 0.7646558637111198 1103 104-105 0.8426743492219124 1103 106-107 0.7836471871159141 1103 108-109 0.802462875901643 1103 110-111 0.7888098864215607 1103 112-113 0.7606912030820752 1103 114-115 0.773222868768169 1103 116-117 0.7508863240457799 1103 118-119 0.8134135551213149 1103 120-121 0.8113619788717408 1103 122-123 0.8396918401529732 1103 124-125 0.9377015018835806 1104 1 0.2562630558883896 1104 2 0.19851526181043155 1104 3 0.18277844156779466 1104 4 0.2539187251866437 1104 5 0.29549545911369535 1104 6 0.13074224563394665 1104 7 0.21566005720849546 1104 8 0.1912548615565015 1104 9 0.22585766448500522 1104 10-11 0.32620621336486266 1104 12-13 0.2506848115600846 1104 14-15 0.2693079410107373 1104 16-17 0.32023322723026126 1104 18-19 0.3408130358038406 1104 20-21 0.35345495415054273 1104 22-23 0.45365373971552714 1104 24-25 0.4169322653028473 1104 26-27 0.37448063359489936 1104 28-29 0.5213945216115121 1104 30-31 0.42893699879732594 1104 32-33 -0.183772139545205 1104 34-35 0.3738685796754453 1104 36-37 0.44363914889935074 1104 38-39 0.5530886843266174 1104 40-41 0.5530338543435676 1104 42-43 0.513545583450572 1104 44-45 0.49361595774349354 1104 46-47 0.5178318778045252 1104 48-49 0.558291031764135 1104 50-51 0.5300252940492882 1104 52-53 0.457140342637139 1104 54-55 0.4705829432600126 1104 56-57 0.5158998349374642 1104 58-59 0.4787894580679648 1104 60-61 0.5667028128508065 1104 62-63 0.5540279819263532 1104 64-65 0.5878561555392139 1104 66-67 0.6896010840453144 1104 68-69 0.6735314652160653 1104 70-71 0.6102819447756573 1104 72-73 0.4662448775981858 1104 74-75 0.49268651665173024 1104 76-77 0.5546097195810731 1104 78-79 0.5288726149050405 1104 80-81 0.5051963809225484 1104 82-83 0.5150842383632508 1104 84-85 0.5543982443883024 1104 86-87 0.5221503575909914 1104 88-89 0.4833208644820388 1104 90-91 0.30976362854895356 1104 92-93 0.2703890076475943 1104 94-95 0.33905501239005176 1104 96-97 0.3568073215811509 1104 98-99 0.34660736088233435 1104 100-101 0.40721373522364956 1104 102-103 0.41772131710992255 1104 104-105 0.4924314167860864 1104 106-107 0.35100318127266306 1104 108-109 0.28364864360031916 1104 110-111 0.31472932938231324 1104 112-113 0.3173327506421302 1104 114-115 0.381885099370578 1104 116-117 0.3830315944247573 1104 118-119 0.4510278620720811 1104 120-121 0.49302395849759506 1104 122-123 0.5377342230144926 1104 124-125 0.5816339827546635 1105 1 0.0026162977401114063 1105 2 0.07205865118677934 1105 3 0.10968156368113213 1105 4 0.06110996236770205 1105 5 0.05224575691184441 1105 6 0.021806072259700215 1105 7 0.013814823093333928 1105 8 0.08977912003291522 1105 9 0.169809452140683 1105 10-11 0.14931733107994916 1105 12-13 0.15591962013964178 1105 14-15 0.20994395366066954 1105 16-17 0.21261293782742285 1105 18-19 0.1845269564124692 1105 20-21 0.15737895556708992 1105 22-23 0.13107221979338846 1105 24-25 0.14837913093087707 1105 26-27 0.20958379186502896 1105 28-29 0.175971326293066 1105 30-31 0.19572984272632965 1105 32-33 -0.2114042170494983 1105 34-35 0.1083814661010507 1105 36-37 0.10932512477336331 1105 38-39 0.03071568502250699 1105 40-41 0.10074854761766261 1105 42-43 0.04869802390950895 1105 44-45 0.020732305809474383 1105 46-47 0.002265021190929417 1105 48-49 0.1294610751275158 1105 50-51 0.1801867613212096 1105 52-53 0.10866041336574028 1105 54-55 0.03640165909990145 1105 56-57 -3.7480306134085595E-4 1105 58-59 5.480397315267282E-4 1105 60-61 -0.03156896727634262 1105 62-63 0.05641271980206852 1105 64-65 0.06684596972803547 1105 66-67 0.08451189318951435 1105 68-69 0.008164709148040572 1105 70-71 5.588660999791273E-4 1105 72-73 -0.005067687874074522 1105 74-75 -0.023985969770897952 1105 76-77 0.06382747008441925 1105 78-79 0.007613046359452369 1105 80-81 0.0063343289508850376 1105 82-83 -0.0831796169898027 1105 84-85 -0.04365250883670768 1105 86-87 -0.03786866186680271 1105 88-89 -0.030830429361152767 1105 90-91 0.03267390277090243 1105 92-93 0.024724602018032726 1105 94-95 0.009489290009746298 1105 96-97 0.003999103590423658 1105 98-99 -0.0273902171085858 1105 100-101 -0.035681333338029475 1105 102-103 -0.007203583273192038 1105 104-105 0.021717047932430233 1105 106-107 0.07049453460119182 1105 108-109 0.051093424022166545 1105 110-111 -0.009343162488605117 1105 112-113 0.02429392172562217 1105 114-115 0.08324494107321456 1105 116-117 0.11332949163548278 1105 118-119 0.13505498461137577 1105 120-121 0.1941366463085501 1105 122-123 0.16946761875542293 1105 124-125 0.2492732916153031 1106 1 0.21882778093749167 1106 2 0.21474754392697548 1106 3 0.240716584878232 1106 4 0.23343019553456656 1106 5 0.0987535595563358 1106 6 0.08358124790712651 1106 7 0.10669144290860544 1106 8 0.08948749225196906 1106 9 0.218814763319493 1106 10-11 0.19817713051062213 1106 12-13 0.1667282182886467 1106 14-15 0.18890765621434724 1106 16-17 0.1947000065464337 1106 18-19 0.1668487292966674 1106 20-21 0.21995565439862474 1106 22-23 0.21685629002769957 1106 24-25 0.2419463876936021 1106 26-27 0.21956826658332318 1106 28-29 0.25902590310889195 1106 30-31 0.28753113797425556 1106 32-33 0.3262025328585878 1106 34-35 0.34033541090762753 1106 36-37 0.3757636014635608 1106 38-39 0.37332140402094893 1106 40-41 0.3815529254425414 1106 42-43 0.37130022838832133 1106 44-45 0.3447922261937606 1106 46-47 0.3260090819790662 1106 48-49 0.34410504389517627 1106 50-51 0.22268344319409294 1106 52-53 0.2526088166401905 1106 54-55 0.32286588798552884 1106 56-57 0.3602747352772653 1106 58-59 0.3947452874324071 1106 60-61 0.4160787700522306 1106 62-63 0.3386722151083461 1106 64-65 0.4230639864512469 1106 66-67 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2216 70-71 -1.2634717162957472 2216 72-73 -1.2706593519234346 2216 74-75 -1.3935399816318963 2216 76-77 -1.484558547984193 2216 78-79 -1.6292689780153182 2216 80-81 -1.4057767554740153 2216 82-83 -1.2387689408639062 2216 84-85 -1.2612708262506906 2216 86-87 -1.2310931154666704 2216 88-89 -1.2298791481151952 2216 90-91 -1.224447312149561 2216 92-93 -1.4263703536725956 2216 94-95 -1.3658679870115797 2216 96-97 -1.3347112750868817 2216 98-99 -1.3683817740025574 2216 100-101 -1.387422362152975 2216 102-103 -1.4347593933741294 2216 104-105 -1.407795333442234 2216 106-107 -1.3761662200437463 2216 108-109 -1.2961768846962194 2216 110-111 -1.3531565396694276 2216 112-113 -1.2788780010978655 2216 114-115 -1.35151300474557 2216 116-117 -1.4411711580710715 2216 118-119 -1.6283227504684668 2216 120-121 -1.6571476788280108 2216 122-123 -1.7637894125475029 2216 124-125 -2.1245638568195524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34254.0 3 5987.0 4 3449.0 5 2903.0 6 3134.0 7 3449.0 8 4060.0 9 4484.0 10 5058.0 11 5549.0 12 6265.0 13 6248.0 14 6313.0 15 5826.0 16 5162.0 17 4858.0 18 4544.0 19 4592.0 20 4860.0 21 5256.0 22 6297.0 23 7982.0 24 10561.0 25 13490.0 26 16679.0 27 19654.0 28 23907.0 29 29824.0 30 35507.0 31 43131.0 32 55497.0 33 74130.0 34 102586.0 35 154331.0 36 296173.0 37 571076.0 38 278719.0 39 2067.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.206748018120656 18.391054997756974 12.687846875951402 24.71435010817097 2 16.088302115739605 19.527562273828426 39.55178764291031 24.832347967521653 3 19.28429402074209 23.243556349637043 29.328713700935456 28.143435928685413 4 12.704288526282955 15.580946336951026 36.847121083371874 34.867644053394145 5 13.896862701436255 37.379027985527195 34.6900378709033 14.03407144213325 6 33.764931662551426 35.58654605808414 16.901920853846768 13.746601425517671 7 29.070862514516595 31.807139958820763 20.91105564578537 18.21094188087727 8 26.08961010792631 35.6621297752939 19.741693742878873 18.506566373900917 9 26.020347748205495 15.868864565077917 19.898799502232634 38.21198818448395 10-11 25.266666648722456 26.031493701986342 27.9616087234696 20.7402309258216 12-13 25.299351631366118 23.57175044456564 27.88760390022563 23.24129402384261 14-15 22.351438783089858 25.63765596236449 25.13416262070599 26.876742633839662 16-17 22.8847034685357 28.502646223137457 26.002849406140488 22.609800902186354 18-19 22.707580012891956 27.553717606940666 27.336680576881413 22.40202180328597 20-21 23.275903368661186 27.108972671647987 27.152977729403617 22.462146230287214 22-23 23.01742525305055 26.980502106556326 27.032345033792115 22.96972760660101 24-25 22.753975273339574 27.111313036592595 27.242391144610643 22.89232054545719 26-27 22.71219015855968 27.426880986701228 26.909277376477288 22.951651478261805 28-29 22.705115352786432 27.535709239660637 27.04741272009204 22.711762687460894 30-31 22.83771174226755 27.170052937080797 26.96760608072629 23.02462923992536 32-33 22.52325574823316 27.337988923796395 27.05646807604723 23.08228725192321 34-35 23.128717635827854 27.339730383007492 26.77289899495824 22.758652986206414 36-37 23.20667512381764 27.186835250179957 26.668969249756024 22.937520376246383 38-39 22.777274328477812 27.287397244802747 26.900497204735952 23.034831221983495 40-41 23.016201032742227 27.21595488051775 26.91906098600181 22.848783100738217 42-43 22.847293676846228 27.20590173210491 26.819334083842207 23.12747050720666 44-45 22.93064019572959 27.351741747532603 26.88592382693627 22.831694229801535 46-47 22.998027189020036 27.46658526187822 26.58692942124154 22.948458127860206 48-49 22.963268024631446 27.33773877451766 26.800230475983476 22.89876272486741 50-51 22.801612616925485 27.470052552592932 26.79681994954521 22.931514880936373 52-53 23.07988357763705 27.307240681342996 26.720771903288327 22.892103837731625 54-55 22.839883492566077 27.228946896393524 26.830782122439302 23.1003874886011 56-57 22.714559888664766 27.600371906137255 26.737905656511664 22.947162548686308 58-59 22.842169861858423 27.301839664553274 26.772755925501222 23.08323454808708 60-61 22.758134416739004 27.318613989400436 27.001025104850047 22.922226489010512 62-63 22.685061415942055 27.408454340804017 27.009432262263687 22.89705198099024 64-65 22.58998660868335 27.257125382690695 26.933114152166134 23.219773856459824 66-67 22.57386604952368 27.5427762342927 26.714216586955054 23.169141129228567 68-69 22.682113460063608 27.18062843632825 27.021325645624188 23.115932457983956 70-71 22.59993189121316 27.047446431578376 26.99012422590813 23.362497451300335 72-73 22.66327087600154 27.290954819968306 26.80332329786979 23.242451006160366 74-75 22.59344141468755 27.437976958066695 26.757693869920406 23.21088775732535 76-77 22.76413863596627 27.320108132825844 26.754132314768736 23.161620916439148 78-79 22.780793202596374 27.35234521616363 26.813361942804015 23.05349963843598 80-81 22.791726508105125 27.364603225371713 26.79867963716115 23.044990629362015 82-83 22.820300373651264 27.45866028939815 26.769167449029823 22.95187188792076 84-85 22.84789018969006 27.35713797680078 26.641503059282783 23.153468774226383 86-87 22.74097066069911 27.134845664733664 26.92574800692868 23.19843566763855 88-89 22.78311587914952 27.200664493649064 26.842326394847593 23.173893232353826 90-91 22.788045271700938 27.328017048830084 26.832301444142843 23.051636235326136 92-93 22.76553869390684 27.034581470665913 26.953763533978766 23.24611630144848 94-95 22.639653318660585 27.03825080181086 27.085071284925093 23.23702459460346 96-97 22.77276098312324 26.887509606485025 27.036507601022038 23.30322180936969 98-99 22.56783880560586 26.94961720927945 26.865884679204953 23.616659305909742 100-101 22.542945830784234 26.88526740546649 27.06356101227116 23.508225751478122 102-103 22.533278039034084 26.583675047981604 27.222291952287435 23.66075496069688 104-105 22.4087112928786 26.769088274061794 27.092189660325587 23.730010772734015 106-107 22.561724247294777 26.819286240317368 26.93072047906509 23.68826903332276 108-109 22.54486912572441 26.805833288755643 26.836612056083485 23.812685529436465 110-111 22.48899039080718 26.88420329792208 27.000974120864658 23.62583219040608 112-113 22.570711767759914 26.9785434796812 27.016238856405085 23.434505896153805 114-115 22.35859275503875 27.13826477212266 26.908423472579685 23.594719000258905 116-117 22.35219792701401 27.03737208498947 27.020327951706495 23.590102036290027 118-119 22.396721576900642 27.122915853645747 26.801974864239515 23.678387705214103 120-121 22.449972447834433 27.192871344430035 26.86636002948579 23.490796178249738 122-123 22.439924025416268 27.17599568778371 26.83042693360827 23.553653353191752 124-125 22.396440659783547 27.276579372528627 26.73379504595034 23.593184921737482 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7770.0 1 4133.0 2 517.5 3 524.0 4 453.5 5 373.0 6 347.5 7 351.5 8 334.5 9 311.0 10 330.5 11 341.5 12 337.0 13 333.0 14 342.5 15 394.0 16 487.5 17 624.5 18 787.0 19 990.5 20 1305.0 21 1846.5 22 2628.5 23 3673.0 24 5110.5 25 7022.5 26 9517.0 27 12590.0 28 15845.0 29 18824.0 30 22034.5 31 26148.0 32 31044.0 33 34285.0 34 36081.0 35 38920.5 36 43180.0 37 46657.5 38 48537.0 39 50801.5 40 53571.5 41 56504.5 42 58379.0 43 60017.5 44 62440.0 45 64991.5 46 65792.0 47 65766.5 48 66727.5 49 66974.5 50 65712.0 51 64479.5 52 64872.5 53 66284.0 54 67482.5 55 64459.5 56 58217.0 57 53149.0 58 49797.5 59 44651.0 60 37321.0 61 30982.0 62 25498.5 63 20169.0 64 15236.0 65 11284.0 66 8261.5 67 6859.0 68 5371.0 69 3752.5 70 2897.5 71 2083.5 72 1546.0 73 1216.5 74 942.5 75 700.5 76 475.0 77 270.0 78 145.5 79 95.0 80 63.0 81 36.5 82 27.5 83 18.5 84 12.5 85 8.5 86 7.5 87 9.0 88 9.0 89 5.0 90 4.5 91 5.0 92 5.0 93 6.5 94 4.5 95 2.5 96 2.0 97 2.5 98 4.0 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4694136933028243 2 0.46331045869555676 3 0.4577961326907448 4 0.45410206963897765 5 0.4631498472585234 6 0.5209164274448541 7 0.5163122329165646 8 0.5265378277410215 9 0.5335511938248115 10-11 0.5488895860614971 12-13 0.5584459665649818 14-15 0.5376200168963232 16-17 0.531677393726089 18-19 0.5415014599579626 20-21 0.5418494514048683 22-23 0.5529316405601699 24-25 0.5455970516023132 26-27 0.5546180606490201 28-29 0.5340062595630727 30-31 0.5402165684616959 32-33 0.5404842541900847 34-35 0.5395741227135623 36-37 0.4861172827542934 38-39 0.45329901245381077 40-41 0.45204088953038285 42-43 0.46464888733750137 44-45 0.4693333875843076 46-47 0.473241599218786 48-49 0.48718802566784913 50-51 0.46962784188553547 52-53 0.4694672304485021 54-55 0.45942901563391725 56-57 0.4418420632787647 58-59 0.46095482428573414 60-61 0.4572072240882892 62-63 0.47203701344103577 64-65 0.47227793059658585 66-67 0.4804155767396092 68-69 0.48919566863076613 70-71 0.48563544844319334 72-73 0.49717270333675617 74-75 0.4859566713172601 76-77 0.4838151854901486 78-79 0.49628934043307266 80-81 0.48882090861102157 82-83 0.4913103858850386 84-85 0.4868132656481046 86-87 0.47765841373720325 88-89 0.4819949225371039 90-91 0.46745958748558514 92-93 0.46818233895223527 94-95 0.4621861786363232 96-97 0.45190704666618836 98-99 0.4161174647805887 100-101 0.41373506179792724 102-103 0.40225134405004226 104-105 0.4074176786079486 106-107 0.4053832670721927 108-109 0.4183124877533779 110-111 0.4409587003750813 112-113 0.43994149460720333 114-115 0.43367764856290236 116-117 0.4274673396642793 118-119 0.47099303910031903 120-121 0.4637119872881401 122-123 0.42987651121977966 124-125 0.43854952881958087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1867862.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.0502008414688 #Duplication Level Percentage of deduplicated Percentage of total 1 87.81331679186476 69.41660328952433 2 8.360124299628493 13.21739009890552 3 1.964415799694522 4.658623905060195 4 0.7178751436620283 2.269926971423264 5 0.34980037501516364 1.3825894949684896 6 0.18503281180194725 0.8776128561123376 7 0.12907957264877132 0.7142636299691434 8 0.09232783197085279 0.5838826928442638 9 0.06058150436225952 0.431008207840344 >10 0.3052517157739885 4.215218807750095 >50 0.01685966374326941 0.8964931995132928 >100 0.005215946093110218 0.6162326798621931 >500 0.0 0.0 >1k 0.0 0.0 >5k 1.1854374084302867E-4 0.720154166226493 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7685 0.4114329645337825 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.944543012278209E-4 14-15 0.0 0.0 0.0 0.0 3.479914469056065E-4 16-17 0.0 0.0 0.0 0.0 3.747600197444993E-4 18-19 0.0 0.0 0.0 0.0 3.747600197444993E-4 20-21 0.0 0.0 0.0 5.3537145677785616E-5 3.747600197444993E-4 22-23 0.0 0.0 0.0 2.1414858271114246E-4 4.2829716542228493E-4 24-25 0.0 0.0 0.0 2.676857283889281E-4 4.2829716542228493E-4 26-27 0.0 0.0 0.0 5.62140029616749E-4 4.2829716542228493E-4 28-29 0.0 0.0 0.0 0.0021682543999503173 4.2829716542228493E-4 30-31 0.0 0.0 0.0 0.006825986073917666 4.550657382611777E-4 32-33 0.0 0.0 0.0 0.016221755140369044 5.353714567778562E-4 34-35 0.0 0.0 0.0 0.03305918745603262 5.353714567778562E-4 36-37 0.0 0.0 0.0 0.06469964055160392 5.353714567778562E-4 38-39 0.0 0.0 0.0 0.1068066056271823 5.353714567778562E-4 40-41 0.0 0.0 0.0 0.1492080250039885 5.353714567778562E-4 42-43 0.0 0.0 0.0 0.19292110444990046 5.62140029616749E-4 44-45 0.0 0.0 0.0 0.2542746733966428 5.889086024556418E-4 46-47 0.0 0.0 0.0 0.329146371627026 6.156771752945346E-4 48-49 0.0 0.0 0.0 0.4077121329091764 6.424457481334274E-4 50-51 0.0 0.0 0.0 0.4910694687294886 6.95982893811213E-4 52-53 0.0 0.0 0.0 0.5734363673547618 8.298257580056771E-4 54-55 0.0 0.0 0.0 0.6689466352439313 8.565943308445699E-4 56-57 0.0 0.0 0.0 0.7874778757745486 9.101314765223554E-4 58-59 0.0 0.0 0.0 0.9169842311691121 9.636686222001411E-4 60-61 0.0 0.0 0.0 1.058616750059694 9.636686222001411E-4 62-63 0.0 0.0 0.0 1.2017483090292538 0.0010707429135557124 64-65 0.0 0.0 0.0 1.3550519256775928 0.0010707429135557124 66-67 0.0 0.0 0.0 1.5367302295351584 0.0010707429135557124 68-69 0.0 0.0 0.0 1.7453912548143278 0.0010707429135557124 70-71 0.0 0.0 0.0 1.978090458502823 0.0010707429135557124 72-73 0.0 0.0 0.0 2.2108699679098347 0.0012045857777501765 74-75 0.0 0.0 0.0 2.4441848487736246 0.0012313543505890693 76-77 0.0 0.0 0.0 2.709836165626797 0.0012313543505890693 78-79 0.0 0.0 0.0 3.019361173362914 0.0012313543505890693 80-81 0.0 0.0 0.0 3.3556815225107637 0.0012313543505890693 82-83 0.0 0.0 0.0 3.7015850207349366 0.0013384286419446405 84-85 0.0 0.0 0.0 4.054742802198449 0.0013384286419446405 86-87 0.0 0.0 0.0 4.434562082209499 0.0013384286419446405 88-89 0.0 0.0 0.0 4.856113567276383 0.0013384286419446405 90-91 0.0 0.0 0.0 5.316238565804111 0.0013384286419446405 92-93 0.0 0.0 0.0 5.790524139363614 0.0013384286419446405 94-95 0.0 0.0 0.0 6.27053818751064 0.0013384286419446405 96-97 0.0 0.0 0.0 6.75384477011685 0.0014455029333002116 98-99 0.0 0.0 0.0 7.28380897518125 0.0014990400789779972 100-101 0.0 0.0 0.0 7.874885832036842 0.0014990400789779972 102-103 0.0 0.0 0.0 8.485557284210504 0.001552577224655783 104-105 0.0 0.0 0.0 9.110041319969035 0.0016328829431724614 106-107 0.0 0.0 0.0 9.713217571747805 0.0016596515160113541 108-109 0.0 0.0 0.0 10.353280916898571 0.0017399572345280325 110-111 0.0 0.0 0.0 11.041313544576633 0.0017667258073669253 112-113 0.0 0.0 0.0 11.767678768559989 0.0017667258073669253 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1650 0.0 59.150974 1 GTATCAA 2530 0.0 41.164185 1 ATCAACG 2900 0.0 35.097015 3 CAACGCA 2900 0.0 34.898346 5 TCAACGC 3015 0.0 33.96031 4 GTACATG 5900 0.0 32.07577 1 AACGCAG 3125 0.0 32.00724 6 TACATGG 5750 0.0 31.981905 2 TATCAAC 3265 0.0 31.7161 2 ACATGGG 5600 0.0 31.248663 3 CATGGGG 3770 0.0 27.317144 4 ACGCAGA 3685 0.0 26.496918 7 CGCAGAG 3835 0.0 25.150038 8 TAGCCCT 1720 0.0 24.57313 4 TATACCG 175 1.7441256E-5 23.81299 5 GAGTACA 4570 0.0 22.91915 1 GTAGCCC 1405 0.0 21.60558 3 AGTACAT 4530 0.0 21.545637 2 GTGTAGC 1480 0.0 20.507391 1 CAGAGTA 4320 0.0 20.121666 10-11 >>END_MODULE