FastQCFastQC Report
Thu 26 May 2016
SRR1364131_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364131_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867862
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49340.26415227677419423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47690.2553186477373596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26660.14273003037697646No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.072.4066541
GTATCAA53600.050.8267561
ATCAACG68650.039.3455053
TCAACGC69700.038.7527854
CAACGCA70450.038.340235
TATCAAC70950.038.2377472
AACGCAG73700.036.730246
GTGGTAT13850.033.5726661
ACGCAGA84600.032.069047
CGCAGAG85800.031.4818338
TGGTATC14050.031.3354022
GTACATG54900.031.2723921
TACATGG54350.031.0884192
ACATGGG52350.030.9123543
CATGGGG35450.027.6915684
GCAGAGT98700.026.9452279
GAGTACT73000.026.61113512-13
CAGAGTA94250.026.10306210-11
AGAGTAC89100.024.907410-11
GTACGGG1451.3233589E-424.667451
Produced by FastQC (version 0.11.5)