FastQCFastQC Report
Thu 26 May 2016
SRR1364130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661905
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN110410.41477813821304665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.053.4349171
GTATCAA41800.046.267461
ATCAACG44450.041.5064773
TCAACGC47650.038.8483544
CAACGCA48050.038.649555
AACGCAG48450.038.3311966
GTACATG87950.037.4837041
TATCAAC54150.034.287272
TACATGG95350.033.4519042
ACATGGG94200.033.1691473
ACGCAGA58800.031.28227
CGCAGAG61450.029.83638
CATGGGG54900.029.381374
GAGTACA68300.024.0467821
TTACGCG650.00205351722.88387782-83
GCAGAGT74350.022.8181279
AGTACAT66700.022.6612912
AGAGTAC63000.021.11841410-11
CATGGGT25800.020.9940784
GTGGTAT17700.020.8442841
Produced by FastQC (version 0.11.5)