##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364130_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2661905 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.267626756026228 31.0 31.0 34.0 28.0 34.0 2 31.329461795218087 33.0 31.0 34.0 28.0 34.0 3 31.415705669435987 33.0 31.0 34.0 28.0 34.0 4 34.90145065282194 37.0 35.0 37.0 32.0 37.0 5 34.95296075554913 37.0 35.0 37.0 32.0 37.0 6 34.98818665579726 37.0 35.0 37.0 32.0 37.0 7 34.89496093962782 37.0 35.0 37.0 32.0 37.0 8 34.87334934943208 37.0 35.0 37.0 32.0 37.0 9 36.4359979037569 39.0 37.0 39.0 32.0 39.0 10-11 36.35251877884447 39.0 37.0 39.0 32.0 39.0 12-13 36.268206228246314 39.0 37.0 39.0 32.0 39.0 14-15 37.57401353541918 40.0 38.0 41.0 32.0 41.0 16-17 37.584369276890044 40.0 38.0 41.0 32.0 41.0 18-19 37.585779169429415 40.0 38.0 41.0 32.0 41.0 20-21 37.598342916069505 40.0 38.0 41.0 32.0 41.0 22-23 37.56140433261142 40.0 38.0 41.0 32.0 41.0 24-25 37.57430017224507 40.0 38.0 41.0 32.0 41.0 26-27 37.53778027390159 40.0 38.0 41.0 32.0 41.0 28-29 37.49262576989036 40.0 38.0 41.0 32.0 41.0 30-31 37.446024933271474 40.0 38.0 41.0 32.0 41.0 32-33 37.35580045118064 40.0 37.5 41.0 31.0 41.0 34-35 37.333659728652975 40.0 37.0 41.0 31.0 41.0 36-37 37.27525193423507 40.0 37.0 41.0 31.0 41.0 38-39 37.19208874847149 40.0 37.0 41.0 31.0 41.0 40-41 37.119969345262135 40.0 37.0 41.0 31.0 41.0 42-43 37.01844844199924 40.0 37.0 41.0 30.0 41.0 44-45 36.92365599072845 40.0 37.0 41.0 30.0 41.0 46-47 36.817458173751504 40.0 36.5 41.0 30.0 41.0 48-49 36.71723464962123 40.0 36.0 41.0 30.0 41.0 50-51 36.59706394480645 40.0 36.0 41.0 30.0 41.0 52-53 36.46136864388474 40.0 36.0 41.0 30.0 41.0 54-55 36.29151096677003 39.5 35.0 41.0 30.0 41.0 56-57 36.10756300469025 39.0 35.0 41.0 29.0 41.0 58-59 35.899966940968966 39.0 35.0 41.0 29.0 41.0 60-61 35.65978199823059 39.0 35.0 41.0 28.5 41.0 62-63 35.398819830159226 38.0 35.0 40.0 28.0 41.0 64-65 35.1226287189062 37.5 34.0 40.0 28.0 41.0 66-67 34.81542635819085 37.0 34.0 40.0 28.0 41.0 68-69 34.48722512636627 36.5 34.0 39.5 28.0 41.0 70-71 34.14788487943785 36.0 34.0 39.0 27.5 41.0 72-73 33.796020331304085 35.5 34.0 39.0 26.5 40.5 74-75 33.52205995330412 35.0 33.5 38.0 26.5 40.0 76-77 33.287221557493595 35.0 33.5 37.0 27.0 39.0 78-79 32.98109248827438 35.0 33.0 37.0 26.0 39.0 80-81 32.773261818134 35.0 33.0 36.0 26.5 39.0 82-83 32.67114998469141 35.0 34.0 36.0 27.0 37.0 84-85 32.46307606770339 35.0 33.5 36.0 27.0 37.0 86-87 32.27232771267194 35.0 33.0 35.0 26.5 37.0 88-89 32.097801950107154 35.0 33.0 35.0 26.0 36.0 90-91 31.961825083915468 35.0 33.0 35.0 26.0 36.0 92-93 31.85489583587694 35.0 33.0 35.0 26.0 36.0 94-95 31.76190359911417 35.0 33.0 35.0 26.0 36.0 96-97 31.684391065796863 35.0 33.0 35.0 25.0 35.0 98-99 31.615431993252955 35.0 33.0 35.0 25.0 35.0 100-101 31.551997911270313 35.0 33.0 35.0 25.0 35.0 102-103 30.47181154098287 34.0 31.0 35.0 23.5 35.0 104-105 31.30657311211332 35.0 33.0 35.0 24.5 35.0 106-107 31.409039203127087 35.0 33.0 35.0 25.0 35.0 108-109 31.402152781560574 35.0 33.0 35.0 25.0 35.0 110-111 31.3479243248726 35.0 33.0 35.0 24.5 35.0 112-113 31.28623467028312 35.0 33.0 35.0 24.0 35.0 114-115 31.20169258482177 35.0 33.0 35.0 24.0 35.0 116-117 31.123978128445607 35.0 33.0 35.0 23.5 35.0 118-119 31.01327714550294 35.0 33.0 35.0 23.0 35.0 120-121 30.897272442104434 35.0 33.0 35.0 19.5 35.0 122-123 30.758719225517062 35.0 33.0 35.0 18.5 35.0 124-125 29.953438608815866 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4151489044971193 1101 2 0.43954003404426345 1101 3 0.47820699666810995 1101 4 0.4666234432410974 1101 5 0.444196971114458 1101 6 0.40969977676314073 1101 7 0.40678085586615964 1101 8 0.41616177676828414 1101 9 0.4380284325730841 1101 10-11 0.4320348392723403 1101 12-13 0.5592634043970364 1101 14-15 0.6237891409867373 1101 16-17 0.5895981916610324 1101 18-19 0.590236647643799 1101 20-21 0.5790770523481328 1101 22-23 0.6231731712500945 1101 24-25 0.5870169661213538 1101 26-27 0.6018807357631601 1101 28-29 0.6828143780964808 1101 30-31 0.7226053326496995 1101 32-33 0.7021763505027891 1101 34-35 0.7178098225631544 1101 36-37 0.714318205115589 1101 38-39 0.651426369609851 1101 40-41 0.6992389482489614 1101 42-43 0.6632795415443553 1101 44-45 0.706273020448954 1101 46-47 0.6639149747371889 1101 48-49 0.5592136117648749 1101 50-51 0.6680492918430545 1101 52-53 0.6414310949254585 1101 54-55 0.6468614745001844 1101 56-57 0.7374832718328079 1101 58-59 0.7058274213204072 1101 60-61 0.6957835341407232 1101 62-63 0.7003522676416338 1101 64-65 0.6849861676478 1101 66-67 0.7479930579743481 1101 68-69 0.7941464436563379 1101 70-71 0.6975932950369241 1101 72-73 0.6619003133078465 1101 74-75 0.5999115889183102 1101 76-77 0.6523749167813193 1101 78-79 0.6678897187524626 1101 80-81 0.616303002203054 1101 82-83 0.6099278843587683 1101 84-85 0.52824501270171 1101 86-87 0.6047862324700262 1101 88-89 0.6346693977186675 1101 90-91 0.6703800355500249 1101 92-93 0.6736377335573849 1101 94-95 0.6965879845898044 1101 96-97 0.6860189189356021 1101 98-99 0.6877774179716241 1101 100-101 0.705319273864152 1101 102-103 0.7156970372280256 1101 104-105 0.6993001770657123 1101 106-107 0.8028253386017958 1101 108-109 0.7551372517992299 1101 110-111 0.6843098487643857 1101 112-113 0.7000615392290364 1101 114-115 0.6676645139956037 1101 116-117 0.7688060019969498 1101 118-119 0.7533269078355005 1101 120-121 0.8006350894402736 1101 122-123 0.8201403921575228 1101 124-125 0.9082482398048413 1102 1 0.2450951742448595 1102 2 0.3088197551189289 1102 3 0.2764322723342332 1102 4 0.269187141793374 1102 5 0.32108742269895174 1102 6 0.2850896232707427 1102 7 0.25176972561706634 1102 8 0.361145466312756 1102 9 0.4679819689992897 1102 10-11 0.4438334069452594 1102 12-13 0.39993237769451184 1102 14-15 0.42604321428378 1102 16-17 0.4644164887257176 1102 18-19 0.39206130341188583 1102 20-21 0.44488952455848363 1102 22-23 0.4510189752109355 1102 24-25 0.4497348765411928 1102 26-27 0.525966537249019 1102 28-29 0.5312172916748068 1102 30-31 0.5125041099770868 1102 32-33 0.4630435962014232 1102 34-35 0.5026618557222378 1102 36-37 0.42496986069517106 1102 38-39 0.4080861725029976 1102 40-41 0.441387410087394 1102 42-43 0.372873967354451 1102 44-45 0.42838063060388976 1102 46-47 0.42339061553592217 1102 48-49 0.41017525953098044 1102 50-51 0.3008147810200228 1102 52-53 0.3359747547125309 1102 54-55 0.31443187134716766 1102 56-57 0.36050813826261674 1102 58-59 0.383485802534679 1102 60-61 0.41552152361627037 1102 62-63 0.3832508953186107 1102 64-65 0.4863701821063344 1102 66-67 0.5300280301235389 1102 68-69 0.4496884371436707 1102 70-71 0.4225270675366417 1102 72-73 0.44677353495017513 1102 74-75 0.38379614657904426 1102 76-77 0.38279488762826475 1102 78-79 0.4131617731216153 1102 80-81 0.4123362951020013 1102 82-83 0.3555777700344862 1102 84-85 0.31216709088540284 1102 86-87 0.29682497465834246 1102 88-89 0.2983594961857605 1102 90-91 0.3086311474957313 1102 92-93 0.3144217865796719 1102 94-95 0.3355728511922962 1102 96-97 0.33601269549315305 1102 98-99 0.3213128153294278 1102 100-101 0.3639921625343199 1102 102-103 0.38043573178722667 1102 104-105 0.39435743273623913 1102 106-107 0.3785717559854227 1102 108-109 0.40287213458986315 1102 110-111 0.38158538394446495 1102 112-113 0.3876910264029867 1102 114-115 0.40022020481210063 1102 116-117 0.32766293189005324 1102 118-119 0.3416297858718558 1102 120-121 0.44645620971747135 1102 122-123 0.42271948743447396 1102 124-125 0.42025941178338044 1103 1 0.21282719197687427 1103 2 0.29078617532064044 1103 3 0.250811273183821 1103 4 0.2538621529389715 1103 5 0.2746456027277233 1103 6 0.22010783475954554 1103 7 0.22897928812074042 1103 8 0.27013639000956147 1103 9 0.2773577313162292 1103 10-11 0.2852218542160614 1103 12-13 0.31438064949572464 1103 14-15 0.4185241405882323 1103 16-17 0.42267138227473566 1103 18-19 0.34834405675345437 1103 20-21 0.2967449514139133 1103 22-23 0.38694250378151906 1103 24-25 0.4047714256953867 1103 26-27 0.3950949093185656 1103 28-29 0.48678588547869595 1103 30-31 0.45961992326937207 1103 32-33 0.27983875270246017 1103 34-35 0.47117301393927846 1103 36-37 0.4599102632826373 1103 38-39 0.4274858242555908 1103 40-41 0.4953018287076887 1103 42-43 0.48786984558562096 1103 44-45 0.5046442892204865 1103 46-47 0.5647704407392737 1103 48-49 0.4432633896779379 1103 50-51 0.3777873418161093 1103 52-53 0.37011477708784213 1103 54-55 0.36536431639726175 1103 56-57 0.4001002513841456 1103 58-59 0.426753229625632 1103 60-61 0.42706226603936415 1103 62-63 0.4108886170739794 1103 64-65 0.3861516862995984 1103 66-67 0.41773632665537264 1103 68-69 0.4170286765427349 1103 70-71 0.41406860907818555 1103 72-73 0.4168591668005064 1103 74-75 0.39587145718376604 1103 76-77 0.3979457527791368 1103 78-79 0.4205843217206322 1103 80-81 0.34947238076749443 1103 82-83 0.27298782715043046 1103 84-85 0.26706316342853853 1103 86-87 0.2576214854548553 1103 88-89 0.32142268542541785 1103 90-91 0.2490806305922746 1103 92-93 0.2527247954709182 1103 94-95 0.26938986147989397 1103 96-97 0.2501064110574589 1103 98-99 0.28759190954970393 1103 100-101 0.33091160298317135 1103 102-103 0.3692581676684874 1103 104-105 0.32477209109207195 1103 106-107 0.34462688674643616 1103 108-109 0.35544151068865304 1103 110-111 0.33694347312608386 1103 112-113 0.32733045574829944 1103 114-115 0.34955252032089135 1103 116-117 0.26947995900119537 1103 118-119 0.34515894528336943 1103 120-121 0.42188310720318967 1103 122-123 0.39630189336982014 1103 124-125 0.4586789237323039 1104 1 0.15570475071757528 1104 2 0.13509334041273036 1104 3 0.11806458449531476 1104 4 0.09211990570592121 1104 5 0.1081606656819929 1104 6 0.050029101740548754 1104 7 0.07606844112883948 1104 8 0.12529458543854588 1104 9 0.1018353513752217 1104 10-11 0.1468660663542849 1104 12-13 0.15377864231060556 1104 14-15 0.13454523441099298 1104 16-17 0.17050731627844584 1104 18-19 0.24401971594571847 1104 20-21 0.2639069034125683 1104 22-23 0.25330509269922885 1104 24-25 0.19701243626043663 1104 26-27 0.20852843707374547 1104 28-29 0.2515111358411204 1104 30-31 0.2084875128422965 1104 32-33 -0.34193410422728476 1104 34-35 0.1826529276650959 1104 36-37 0.253444191502318 1104 38-39 0.360059807991405 1104 40-41 0.32548559403004873 1104 42-43 0.3115914951466294 1104 44-45 0.2967975256148705 1104 46-47 0.31533540969689255 1104 48-49 0.2681664878177017 1104 50-51 0.2649340713834789 1104 52-53 0.25433199219884983 1104 54-55 0.22025156573606353 1104 56-57 0.3005111828535405 1104 58-59 0.2767500565687584 1104 60-61 0.31761797131924396 1104 62-63 0.3079626877597974 1104 64-65 0.29583618554589464 1104 66-67 0.34502428609958 1104 68-69 0.30657106404962775 1104 70-71 0.3343956234954035 1104 72-73 0.3016617490918847 1104 74-75 0.4173786015067549 1104 76-77 0.344865456308483 1104 78-79 0.3344512808030373 1104 80-81 0.3223231704465803 1104 82-83 0.3454761053838382 1104 84-85 0.3525737494880232 1104 86-87 0.3025019024192588 1104 88-89 0.29848230840033096 1104 90-91 0.33563152601243473 1104 92-93 0.31532323234893056 1104 94-95 0.28206581160438304 1104 96-97 0.3202620804035021 1104 98-99 0.30677551086210997 1104 100-101 0.27560398542141584 1104 102-103 0.29992605081098134 1104 104-105 0.30700544511208605 1104 106-107 0.3032485767742159 1104 108-109 0.3082742263061071 1104 110-111 0.25891079311279697 1104 112-113 0.28320951200701217 1104 114-115 0.22224144289088343 1104 116-117 0.3033169017897457 1104 118-119 0.3469051567770869 1104 120-121 0.33269404163959493 1104 122-123 0.42481902087148526 1104 124-125 0.4911671113069396 1105 1 0.045921868429626755 1105 2 0.14336709451653462 1105 3 0.1729894624542574 1105 4 0.15436061282556324 1105 5 0.17274135276342406 1105 6 0.09204471540628134 1105 7 0.10333576567811065 1105 8 0.14040782677552244 1105 9 0.07581474235548313 1105 10-11 0.2049758009389535 1105 12-13 0.17959163126597133 1105 14-15 0.29701740522853726 1105 16-17 0.3063389369701568 1105 18-19 0.24662102053878954 1105 20-21 0.2513863089219228 1105 22-23 0.21932904274675025 1105 24-25 0.28668957594790356 1105 26-27 0.31058429698835255 1105 28-29 0.2818735550902147 1105 30-31 0.3038523277330327 1105 32-33 -0.05388264390756348 1105 34-35 0.3280410421776949 1105 36-37 0.33413439105243015 1105 38-39 0.3855320267085105 1105 40-41 0.44445570563073034 1105 42-43 0.402743669408693 1105 44-45 0.37256120190244957 1105 46-47 0.37138705524595395 1105 48-49 0.3424730752815819 1105 50-51 0.3779074162710856 1105 52-53 0.4388565717740178 1105 54-55 0.3915447863024184 1105 56-57 0.34865246746146994 1105 58-59 0.33001377498406725 1105 60-61 0.24819558035610356 1105 62-63 0.26597400930612025 1105 64-65 0.33333394813203654 1105 66-67 0.341698915239526 1105 68-69 0.40335100588814754 1105 70-71 0.3226474592362507 1105 72-73 0.30934485247413335 1105 74-75 0.33684314674020044 1105 76-77 0.37678945442805656 1105 78-79 0.37587345734596056 1105 80-81 0.3873831517140047 1105 82-83 0.32218447784288173 1105 84-85 0.20847381480923133 1105 86-87 0.21528986537816053 1105 88-89 0.23775713818770328 1105 90-91 0.20825334913354254 1105 92-93 0.2098762889448551 1105 94-95 0.2469231883296743 1105 96-97 0.22387253328260215 1105 98-99 0.2216354285199742 1105 100-101 0.19668331652058413 1105 102-103 0.16786360172085324 1105 104-105 0.21946258926576334 1105 106-107 0.21615847795914078 1105 108-109 0.2236384162468532 1105 110-111 0.1762606919996017 1105 112-113 0.21636182213930866 1105 114-115 0.26392295377848285 1105 116-117 0.23583981695457368 1105 118-119 0.23142079996540588 1105 120-121 0.23814799748680926 1105 122-123 0.29358801401070167 1105 124-125 0.3448010362994083 1106 1 0.2058214368470601 1106 2 0.2014662106218097 1106 3 0.25080345263337733 1106 4 0.25211021708763326 1106 5 0.17706422062172322 1106 6 0.20457423330062596 1106 7 0.24728507588478976 1106 8 0.22216261509878876 1106 9 0.22813956102281452 1106 10-11 0.2380972055112096 1106 12-13 0.2327297949216458 1106 14-15 0.2779767868901928 1106 16-17 0.21867007054308374 1106 18-19 0.35319094230540315 1106 20-21 0.30278851373561366 1106 22-23 0.3169837697775293 1106 24-25 0.331878858585128 1106 26-27 0.31974993688534425 1106 28-29 0.2568359210953304 1106 30-31 0.3493188797958595 1106 32-33 0.31052930995776507 1106 34-35 0.3394501572804245 1106 36-37 0.3470314205756395 1106 38-39 0.38158482659194704 1106 40-41 0.3537970540628166 1106 42-43 0.3278185692850428 1106 44-45 0.31913632591185603 1106 46-47 0.312847579467622 1106 48-49 0.2615316255446771 1106 50-51 0.2177403734458636 1106 52-53 0.13637124925609356 1106 54-55 0.2918892508305575 1106 56-57 0.2516746209748817 1106 58-59 0.3045506338921484 1106 60-61 0.2945469490273851 1106 62-63 0.2845582950502177 1106 64-65 0.36726744290604074 1106 66-67 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116-117 -1.2100429571024023 2216 118-119 -1.3131869256525732 2216 120-121 -1.3171341510413548 2216 122-123 -1.4451457717107523 2216 124-125 -1.663966364151218 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41448.0 3 7036.0 4 4050.0 5 3352.0 6 3754.0 7 4458.0 8 5129.0 9 5836.0 10 6605.0 11 7209.0 12 7990.0 13 8229.0 14 8205.0 15 7371.0 16 6585.0 17 6155.0 18 6102.0 19 5950.0 20 6247.0 21 6778.0 22 8208.0 23 10319.0 24 13577.0 25 17511.0 26 21945.0 27 25768.0 28 31395.0 29 39203.0 30 47820.0 31 58260.0 32 75565.0 33 101504.0 34 142582.0 35 217993.0 36 417981.0 37 846770.0 38 424749.0 39 2266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21729884706058 18.562603938549152 12.754123383314422 24.465973831075843 2 15.917418104248659 19.65815062568218 39.93737894344328 24.487052326625875 3 19.06796068139567 23.89828730236725 29.474725425683594 27.559026590553483 4 12.727178037236103 15.79632228014706 37.25768871089601 34.21881097172082 5 13.734719857608644 37.6532448872452 34.806134815117304 13.80590044002885 6 33.093989207233946 36.04644137050757 17.233305766086243 13.62626365617223 7 29.01322748026111 31.844736743726585 21.171596243333678 17.970439532678625 8 26.000972819219527 35.79181668702185 19.942718470800695 18.264492022957928 9 26.22178278846401 15.952515472919181 20.031179990422427 37.794521748194384 10-11 24.94562462367858 26.16305511423173 28.243163509910712 20.64815675217898 12-13 25.277084675203003 23.790126231765527 28.104955772605273 22.8278333204262 14-15 22.45386758772547 25.687796191190206 25.30899250380758 26.549343717276745 16-17 22.669690281529288 28.744867483639396 26.104963925094722 22.480478309736597 18-19 22.683603618371663 27.833923093643943 27.40001159565023 22.08246169233416 20-21 23.229845030999655 27.224285886144045 27.23302988691911 22.312839195937194 22-23 23.022273545176507 27.074230859235783 27.075458530492615 22.828037065095096 24-25 22.80113529269784 27.32285320479576 27.17142292712574 22.70458857538066 26-27 22.696835406338707 27.520777744850445 27.074332225199534 22.708054623611314 28-29 22.611802291403826 27.497148066926623 27.204108520939634 22.686941120729916 30-31 22.784758252968796 27.341751450263125 27.06858189402343 22.80490840274465 32-33 22.595794843476575 27.46407162219992 27.081303948278396 22.858829586045108 34-35 23.08478063892075 27.41538535915828 26.890517940445402 22.609316061475564 36-37 23.119818011528302 27.269787867611388 26.80688904584782 22.803505075012488 38-39 22.724353120013767 27.473114911487528 27.056328109786566 22.746203858712143 40-41 22.930511748176695 27.249686586979376 27.069695810115107 22.75010585472882 42-43 22.77465969657414 27.314366181979654 26.929446024086335 22.98152809735987 44-45 22.771134428414978 27.42214580215872 27.027036208125526 22.77968356130077 46-47 22.902003503966263 27.525008176739107 26.69572735420728 22.877260965087352 48-49 22.825248426951763 27.36989732598316 26.896307642650253 22.908546604414823 50-51 22.66333432664393 27.509054444429555 26.909010547524247 22.918600681402264 52-53 22.910448268209056 27.33126720203397 26.889411775363136 22.86887275439383 54-55 22.828204754183155 27.327712329819214 26.83864690578419 23.005436010213444 56-57 22.68724216555931 27.561061893472928 26.880459156383164 22.8712367845846 58-59 22.752256702171923 27.31889534582009 26.81074461028662 23.11810334172137 60-61 22.799887307975865 27.370707508530685 27.006307696401045 22.823097487092404 62-63 22.651558959013066 27.420968757407525 27.00163663897164 22.925835644607773 64-65 22.607028770876656 27.287132390270525 26.881762843244907 23.224075995607915 66-67 22.57410804038595 27.553405087464267 26.749068282185267 23.123418589964512 68-69 22.479622949294125 27.25174408980116 27.09273315893222 23.1758998019725 70-71 22.57567486760862 27.13779802217018 27.077453676229652 23.209073433991552 72-73 22.64701379540649 27.24448841617392 26.914190755629757 23.194307032789833 74-75 22.57501666219007 27.41710289107637 26.756825269072426 23.251055177661133 76-77 22.695088471313476 27.3015234864936 26.878159774029854 23.125228268163067 78-79 22.72743060864746 27.181562791533537 27.000663342028137 23.09034325779087 80-81 22.59072554766791 27.35055125434714 26.93108905389019 23.12763414409476 82-83 22.643277048320368 27.36758631846257 26.94790737200717 23.04122926120989 84-85 22.71227954074295 27.197636933094323 26.883321400844608 23.20676212531812 86-87 22.5897630539992 27.030098872514362 27.129884880881516 23.250253192604927 88-89 22.744151044821308 27.068752981031064 27.01622454763355 23.17087142651408 90-91 22.73082067897227 27.132224611008617 27.095556152752152 23.04139855726696 92-93 22.682913204596016 26.950743293075558 27.072958444583843 23.293385057744583 94-95 22.64355103295527 26.865781767971963 27.103873726155186 23.38679347291758 96-97 22.670401476312765 26.80636037202176 27.14362538186196 23.37961276980352 98-99 22.656024681237465 26.794406204865513 26.96865821612946 23.580910897767563 100-101 22.50409304220549 26.755292661948666 27.108275112794434 23.63233918305141 102-103 22.57239544169409 26.621123590758838 27.194730974666708 23.611749992880355 104-105 22.538206423703713 26.705567798342813 27.144158002620905 23.612067775332573 106-107 22.559375090178012 26.673642633467704 27.099273426904087 23.6677088494502 108-109 22.544679656679754 26.722005445347897 26.954509803625683 23.77880509434667 110-111 22.536858285286705 26.82885827849455 26.98253076798699 23.651752668231754 112-113 22.546188989733473 26.854733799539076 27.113307240150675 23.485769970576776 114-115 22.464295537975147 26.908699735388346 26.983917663629654 23.643087063006853 116-117 22.431890862388755 26.86285207202394 27.085625395215608 23.619631670371696 118-119 22.40354923855109 26.904698007856247 26.840512622496703 23.85124013109596 120-121 22.41688016220125 26.957107400960467 26.99437790898674 23.631634527851542 122-123 22.489145700867493 26.977029350703987 26.952316542772493 23.581508405656027 124-125 22.50264932485373 26.92949805552668 26.882690329252075 23.685162290367515 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11058.0 1 5831.5 2 610.0 3 539.5 4 455.0 5 410.0 6 353.0 7 346.5 8 373.0 9 372.5 10 343.5 11 327.5 12 359.5 13 400.0 14 412.5 15 463.0 16 556.0 17 675.5 18 898.0 19 1154.5 20 1445.5 21 2064.0 22 2960.5 23 4162.0 24 5813.0 25 8308.5 26 12344.5 27 16861.5 28 20501.5 29 24539.0 30 30395.5 31 36804.0 32 42811.5 33 48004.5 34 54081.5 35 59702.5 36 66434.0 37 73189.0 38 75293.0 39 77189.5 40 79606.0 41 82900.5 42 86507.0 43 90227.5 44 93807.0 45 97326.5 46 97911.0 47 95946.0 48 96566.5 49 96723.5 50 94897.5 51 92818.0 52 92729.5 53 93137.5 54 91373.5 55 85330.0 56 77998.0 57 71774.5 58 66445.0 59 59106.5 60 49089.5 61 40360.5 62 33017.0 63 26118.5 64 20035.0 65 15614.0 66 12138.0 67 9846.5 68 7700.0 69 5372.5 70 3879.0 71 2960.0 72 2272.5 73 1924.0 74 1531.0 75 1184.0 76 858.5 77 533.5 78 349.0 79 207.5 80 117.5 81 72.5 82 55.0 83 43.5 84 34.5 85 23.0 86 19.5 87 19.0 88 16.5 89 14.5 90 7.0 91 4.5 92 5.5 93 5.5 94 6.5 95 5.5 96 3.5 97 4.5 98 4.5 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4697012102235053 2 0.46271373320986287 3 0.45707867110208666 4 0.4549749145818502 5 0.46230049532195927 6 0.519740561740558 7 0.5148944083278704 8 0.5233470014895347 9 0.5279301853371927 10-11 0.5472396648265058 12-13 0.5556171238267331 14-15 0.5344856409225723 16-17 0.528982063597311 18-19 0.5394820626581339 20-21 0.5400455688689115 22-23 0.549099235322072 24-25 0.5440464629654327 26-27 0.5507897539544049 28-29 0.5311797378193437 30-31 0.536533046821731 32-33 0.5389373399877156 34-35 0.5366081809831681 36-37 0.4863434269818044 38-39 0.4548997804204132 40-41 0.4525706214158657 42-43 0.4656251819655472 44-45 0.4700956645710496 46-47 0.47415290928864856 48-49 0.4879024608316225 50-51 0.4701707987324867 52-53 0.4699266127078164 54-55 0.46055362606854866 56-57 0.4443246471981532 58-59 0.46198117513585196 60-61 0.45852500370974925 62-63 0.47233090587380094 64-65 0.4731198145688896 66-67 0.47958135245247296 68-69 0.48951784530251835 70-71 0.48615559157821187 72-73 0.49714396268837546 74-75 0.48624950928000815 76-77 0.4839579173561791 78-79 0.4957351971614314 80-81 0.4897620313271886 82-83 0.49192213846850286 84-85 0.4874140887822819 86-87 0.4784543400309177 88-89 0.4832441428225275 90-91 0.46917527109344626 92-93 0.46879960028626116 94-95 0.46201874221657047 96-97 0.4530589934652063 98-99 0.41874146522884925 100-101 0.4156797481502909 102-103 0.4052924503316234 104-105 0.40989441771964064 106-107 0.4094248292106593 108-109 0.42082643820872645 110-111 0.44285953105013137 112-113 0.4428219639694129 114-115 0.43600353881900367 116-117 0.430255775469072 118-119 0.4713917288558382 120-121 0.4642164164386032 122-123 0.43042482733230525 124-125 0.4395911950276212 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2661905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.22604846846518 #Duplication Level Percentage of deduplicated Percentage of total 1 84.0586287372858 55.66870820948209 2 9.735193527130331 12.894467967552437 3 2.849556844569909 5.661446691064005 4 1.1857774281416735 3.141174137156898 5 0.5928670731058295 1.9631621759431876 6 0.3297542316637257 1.3102991837306037 7 0.22544054465181196 1.045102550580766 8 0.1569395672343513 0.8314789909025679 9 0.12351293107805983 0.7361796024052011 >10 0.6339565288820385 8.212617217442277 >50 0.0709835741580902 3.2525537993531284 >100 0.03696252175523288 4.143038850048882 >500 2.8432689545452417E-4 0.10026893399305711 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.4216344772726209E-4 1.0395016903449235 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11041 0.41477813821304665 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.756708071850798E-5 0.0 6 0.0 0.0 0.0 3.756708071850798E-5 0.0 7 0.0 0.0 0.0 3.756708071850798E-5 0.0 8 0.0 0.0 0.0 3.756708071850798E-5 0.0 9 0.0 0.0 0.0 1.1270124215552396E-4 0.0 10-11 0.0 0.0 0.0 2.254024843110479E-4 0.0 12-13 0.0 0.0 0.0 3.0053664574806386E-4 0.0 14-15 0.0 0.0 0.0 3.0053664574806386E-4 0.0 16-17 0.0 0.0 0.0 3.0053664574806386E-4 0.0 18-19 0.0 0.0 0.0 3.1932018610731787E-4 3.756708071850798E-5 20-21 0.0 0.0 0.0 3.568872668258259E-4 7.513416143701597E-5 22-23 0.0 0.0 0.0 3.756708071850799E-4 9.391770179626997E-5 24-25 0.0 0.0 0.0 4.6958850898134986E-4 1.5026832287403193E-4 26-27 0.0 0.0 0.0 7.513416143701598E-4 1.5026832287403193E-4 28-29 0.0 0.0 0.0 0.0022540248431104793 1.5026832287403193E-4 30-31 0.0 0.0 0.0 0.007025044094360994 1.5026832287403193E-4 32-33 0.0 0.0 0.0 0.018088549365961597 1.5026832287403193E-4 34-35 0.0 0.0 0.0 0.038055452767848594 1.5026832287403193E-4 36-37 0.0 0.0 0.0 0.07139623690552442 1.5026832287403193E-4 38-39 0.0 0.0 0.0 0.12002682289563302 1.5026832287403193E-4 40-41 0.0 0.0 0.0 0.1701600921144819 1.5026832287403193E-4 42-43 1.878354035925399E-5 0.0 0.0 0.2203497119544086 1.5026832287403193E-4 44-45 3.756708071850798E-5 0.0 0.0 0.2838756454494056 1.5026832287403193E-4 46-47 3.756708071850798E-5 0.0 0.0 0.3653398599874902 1.5026832287403193E-4 48-49 3.756708071850798E-5 0.0 0.0 0.45172536209969927 1.5026832287403193E-4 50-51 3.756708071850798E-5 0.0 0.0 0.5432012036492662 1.5026832287403193E-4 52-53 3.756708071850798E-5 0.0 0.0 0.635109066627096 1.5026832287403193E-4 54-55 3.756708071850798E-5 0.0 0.0 0.7374230109639526 1.5026832287403193E-4 56-57 3.756708071850798E-5 0.0 0.0 0.8652074360279574 1.5026832287403193E-4 58-59 3.756708071850798E-5 0.0 0.0 1.0117378343704977 1.5026832287403193E-4 60-61 3.756708071850798E-5 0.0 0.0 1.174290592639482 1.5026832287403193E-4 62-63 3.756708071850798E-5 0.0 0.0 1.3353406676797257 1.5026832287403193E-4 64-65 3.756708071850798E-5 1.878354035925399E-5 0.0 1.5031903843300194 1.5026832287403193E-4 66-67 3.756708071850798E-5 3.756708071850798E-5 0.0 1.7001545885371567 1.5026832287403193E-4 68-69 3.756708071850798E-5 3.756708071850798E-5 0.0 1.9264211157047302 1.5026832287403193E-4 70-71 3.756708071850798E-5 3.756708071850798E-5 0.0 2.1788343310523857 1.5026832287403193E-4 72-73 5.6350621077761975E-5 3.756708071850798E-5 0.0 2.4310409274560887 1.8783540359253994E-4 74-75 7.513416143701597E-5 3.756708071850798E-5 0.0 2.6978235511785735 1.8783540359253994E-4 76-77 7.513416143701597E-5 3.756708071850798E-5 0.0 2.994058766184368 2.0661894395179395E-4 78-79 7.513416143701597E-5 3.756708071850798E-5 0.0 3.3361633867474607 2.2540248431104793E-4 80-81 7.513416143701597E-5 3.756708071850798E-5 0.0 3.709317199524401 2.2540248431104793E-4 82-83 7.513416143701597E-5 3.756708071850798E-5 0.0 4.093891404839766 2.629695650295559E-4 84-85 7.513416143701597E-5 3.756708071850798E-5 0.0 4.480212479408544 2.629695650295559E-4 86-87 7.513416143701597E-5 3.756708071850798E-5 0.0 4.889017451787348 2.629695650295559E-4 88-89 7.513416143701597E-5 3.756708071850798E-5 0.0 5.34949594369446 3.0053664574806386E-4 90-91 7.513416143701597E-5 3.756708071850798E-5 0.0 5.858473536809164 3.381037264665719E-4 92-93 7.513416143701597E-5 3.756708071850798E-5 0.0 6.377387622773916 3.381037264665719E-4 94-95 7.513416143701597E-5 3.756708071850798E-5 0.0 6.902988649106561 3.381037264665719E-4 96-97 7.513416143701597E-5 3.756708071850798E-5 0.0 7.4383195493453 3.381037264665719E-4 98-99 7.513416143701597E-5 3.756708071850798E-5 0.0 8.017359748000022 4.1323788790358784E-4 100-101 7.513416143701597E-5 3.756708071850798E-5 0.0 8.659550209342557 4.883720493406038E-4 102-103 7.513416143701597E-5 3.756708071850798E-5 0.0 9.336095766002167 4.883720493406038E-4 104-105 1.1270124215552396E-4 3.756708071850798E-5 0.0 10.001840786955206 4.883720493406038E-4 106-107 1.1270124215552396E-4 3.756708071850798E-5 0.0 10.661650209154722 4.883720493406038E-4 108-109 1.1270124215552396E-4 3.756708071850798E-5 0.0 11.357524028844004 4.883720493406038E-4 110-111 1.1270124215552396E-4 3.756708071850798E-5 0.0 12.100112513406753 4.883720493406038E-4 112-113 1.1270124215552396E-4 3.756708071850798E-5 0.0 12.876793123721544 4.883720493406038E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2450 0.0 53.434917 1 GTATCAA 4180 0.0 46.26746 1 ATCAACG 4445 0.0 41.506477 3 TCAACGC 4765 0.0 38.848354 4 CAACGCA 4805 0.0 38.64955 5 AACGCAG 4845 0.0 38.331196 6 GTACATG 8795 0.0 37.483704 1 TATCAAC 5415 0.0 34.28727 2 TACATGG 9535 0.0 33.451904 2 ACATGGG 9420 0.0 33.169147 3 ACGCAGA 5880 0.0 31.2822 7 CGCAGAG 6145 0.0 29.8363 8 CATGGGG 5490 0.0 29.38137 4 GAGTACA 6830 0.0 24.046782 1 TTACGCG 65 0.002053517 22.883877 82-83 GCAGAGT 7435 0.0 22.818127 9 AGTACAT 6670 0.0 22.661291 2 AGAGTAC 6300 0.0 21.118414 10-11 CATGGGT 2580 0.0 20.994078 4 GTGGTAT 1770 0.0 20.844284 1 >>END_MODULE