FastQCFastQC Report
Thu 26 May 2016
SRR1364130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661905
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.21548477500136182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56430.21199103649454054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.11750982848749297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42700.073.389291
GTATCAA76750.049.9122851
ATCAACG93100.040.642293
TCAACGC96300.039.2925074
CAACGCA97800.038.689865
AACGCAG99850.038.253756
TATCAAC99950.037.7399832
TGGTATC21350.034.2769742
GTACATG86050.034.2019541
GTGGTAT22750.033.519951
ACGCAGA117300.032.007457
CGCAGAG117900.031.6931618
TACATGG91400.031.5711782
ACATGGG90350.030.685173
GCAGAGT133550.027.3109179
CATGGGG51950.025.8822364
GAGTACT90500.025.54170612-13
CAGAGTA128500.025.46684610-11
AGAGTAC121250.024.53600710-11
GTACTTT99200.023.3896514-15
Produced by FastQC (version 0.11.5)