FastQCFastQC Report
Thu 26 May 2016
SRR1364129_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364129_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976281
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN122840.41272984640899163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.053.9764331
GTATCAA41100.047.4965551
ATCAACG47600.040.3909763
TCAACGC49400.039.0450134
CAACGCA49550.038.9294435
GTACATG103400.037.7583851
TACATGG101150.037.5404552
AACGCAG51850.037.087776
TATCAAC54000.035.7105062
ACATGGG106000.034.591043
ACGCAGA62550.029.9829797
CGCAGAG63550.029.042758
CATGGGA49000.027.2144434
CATGGGG64050.027.1400894
GAGTACA72900.026.696251
TAAGGTG17450.025.9295545
GTATAGG8000.024.5501421
GCAGAGT79000.022.307779
GTAAGGT18200.022.2425734
GGTAAGG19000.021.6164463
Produced by FastQC (version 0.11.5)