##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364129_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2976281 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23140926545578 33.0 31.0 34.0 28.0 34.0 2 31.21766325155454 33.0 31.0 34.0 28.0 34.0 3 31.31727313382036 33.0 31.0 34.0 28.0 34.0 4 34.80756521309648 37.0 35.0 37.0 32.0 37.0 5 34.83110398514119 37.0 35.0 37.0 32.0 37.0 6 34.886175734078876 37.0 35.0 37.0 32.0 37.0 7 34.782969753191985 37.0 35.0 37.0 32.0 37.0 8 34.71621463161576 37.0 35.0 37.0 32.0 37.0 9 36.26643922398456 39.0 37.0 39.0 32.0 39.0 10-11 36.185934224624624 39.0 37.0 39.0 32.0 39.0 12-13 36.10376086800944 39.0 37.0 39.0 31.0 39.0 14-15 37.39329350958461 40.0 38.0 41.0 31.5 41.0 16-17 37.39461898926882 40.0 38.0 41.0 31.0 41.0 18-19 37.39405301448351 40.0 38.0 41.0 31.0 41.0 20-21 37.40657316967047 40.0 38.0 41.0 31.0 41.0 22-23 37.37991960436531 40.0 38.0 41.0 31.0 41.0 24-25 37.38830641327213 40.0 38.0 41.0 31.0 41.0 26-27 37.35598688430292 40.0 37.5 41.0 31.0 41.0 28-29 37.31584383329397 40.0 37.0 41.0 31.0 41.0 30-31 37.25904207297631 40.0 37.0 41.0 31.0 41.0 32-33 37.15882018532524 40.0 37.0 41.0 31.0 41.0 34-35 37.1373862212607 40.0 37.0 41.0 31.0 41.0 36-37 37.07391422382497 40.0 37.0 41.0 30.0 41.0 38-39 36.97792328748529 40.0 37.0 41.0 30.0 41.0 40-41 36.89044162832744 40.0 37.0 41.0 30.0 41.0 42-43 36.76619260748565 40.0 36.0 41.0 30.0 41.0 44-45 36.671708921301445 40.0 36.0 41.0 30.0 41.0 46-47 36.55048330449981 40.0 36.0 41.0 30.0 41.0 48-49 36.41857119673848 40.0 35.5 41.0 30.0 41.0 50-51 36.27598553362401 40.0 35.0 41.0 29.5 41.0 52-53 36.10513221029869 39.0 35.0 41.0 29.0 41.0 54-55 35.914927219573684 39.0 35.0 41.0 29.0 41.0 56-57 35.7081962355033 39.0 35.0 41.0 28.0 41.0 58-59 35.4726791589907 38.5 35.0 41.0 28.0 41.0 60-61 35.20874709074849 38.0 34.5 40.0 28.0 41.0 62-63 34.922383840773094 37.5 34.0 40.0 28.0 41.0 64-65 34.61356168990764 37.0 34.0 40.0 27.5 41.0 66-67 34.299836104185054 36.5 34.0 39.5 27.0 41.0 68-69 33.964771975495594 36.0 34.0 39.0 26.0 41.0 70-71 33.600879083661795 35.5 33.0 39.0 26.0 40.5 72-73 33.26888472560219 35.0 33.0 38.0 26.0 40.0 74-75 33.01427183790778 35.0 33.0 37.0 26.0 39.5 76-77 32.80737336293179 35.0 33.0 37.0 26.0 39.0 78-79 32.521592551240964 35.0 33.0 36.0 26.0 39.0 80-81 32.35040894996138 35.0 33.0 36.0 26.0 38.0 82-83 32.27298934475609 35.0 33.0 36.0 26.0 37.0 84-85 32.09191890819449 35.0 33.0 35.0 26.0 37.0 86-87 31.930477498596403 35.0 33.0 35.0 26.0 36.5 88-89 31.766258293487745 35.0 33.0 35.0 25.0 36.0 90-91 31.63938116058262 35.0 33.0 35.0 25.0 36.0 92-93 31.53056667028416 35.0 33.0 35.0 25.0 36.0 94-95 31.439094124513108 35.0 33.0 35.0 25.0 35.5 96-97 31.370672997610104 35.0 33.0 35.0 24.5 35.0 98-99 31.298103068897056 35.0 33.0 35.0 24.0 35.0 100-101 31.230689071361205 35.0 33.0 35.0 24.0 35.0 102-103 30.12355704988877 34.0 30.5 35.0 20.0 35.0 104-105 30.97569449927611 35.0 32.5 35.0 23.0 35.0 106-107 31.084151496448083 35.0 33.0 35.0 23.0 35.0 108-109 31.078893256382713 35.0 33.0 35.0 23.0 35.0 110-111 31.011096566486835 35.0 33.0 35.0 23.0 35.0 112-113 30.943298532631832 35.0 33.0 35.0 21.0 35.0 114-115 30.847653665766103 35.0 33.0 35.0 19.5 35.0 116-117 30.7660657713435 35.0 33.0 35.0 19.0 35.0 118-119 30.63724023370105 35.0 33.0 35.0 17.5 35.0 120-121 30.51018200230422 35.0 32.5 35.0 10.0 35.0 122-123 30.36943067539658 35.0 32.0 35.0 2.0 35.0 124-125 29.542281961951844 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.413572041198389 1101 2 0.4428053821706932 1101 3 0.4066749428220575 1101 4 0.5219906171445956 1101 5 0.4705438674361133 1101 6 0.3536905909562833 1101 7 0.44116305067912975 1101 8 0.42125410124925367 1101 9 0.4553522251742237 1101 10-11 0.5144012460454164 1101 12-13 0.6401382522248298 1101 14-15 0.640756762093396 1101 16-17 0.5522327026704161 1101 18-19 0.5383307393823884 1101 20-21 0.5312162977942592 1101 22-23 0.6669459003510028 1101 24-25 0.6048904868453633 1101 26-27 0.5559640911885566 1101 28-29 0.6068019879097548 1101 30-31 0.634456490100753 1101 32-33 0.6152130707285295 1101 34-35 0.6240772547026623 1101 36-37 0.6498069417764611 1101 38-39 0.5801443072388892 1101 40-41 0.6470824170846967 1101 42-43 0.6011574142848559 1101 44-45 0.6556704200109564 1101 46-47 0.6184282791542799 1101 48-49 0.5380720366319167 1101 50-51 0.569093761532244 1101 52-53 0.5690967523852208 1101 54-55 0.5648423563360794 1101 56-57 0.4639911312216114 1101 58-59 0.45031328710288676 1101 60-61 0.5090222145587617 1101 62-63 0.468604718424082 1101 64-65 0.4718423726014649 1101 66-67 0.4634988383853553 1101 68-69 0.565552930633558 1101 70-71 0.5913615117907653 1101 72-73 0.4945995648768715 1101 74-75 0.5427904647369175 1101 76-77 0.5189566885768144 1101 78-79 0.5443312075109432 1101 80-81 0.5102433284692083 1101 82-83 0.5771513576180922 1101 84-85 0.542269894095817 1101 86-87 0.5115996333121942 1101 88-89 0.5872405639951985 1101 90-91 0.5308728716087003 1101 92-93 0.5522965278259981 1101 94-95 0.5580196444056469 1101 96-97 0.5241742262654938 1101 98-99 0.5219590792926141 1101 100-101 0.5355541699420812 1101 102-103 0.558358532672397 1101 104-105 0.5163532575729413 1101 106-107 0.5820513163720946 1101 108-109 0.5946881254453587 1101 110-111 0.5210507076355562 1101 112-113 0.559972940284716 1101 114-115 0.5453129604888289 1101 116-117 0.5527584781522812 1101 118-119 0.5281593967427902 1101 120-121 0.5729819240116072 1101 122-123 0.5932929053771687 1101 124-125 0.7066609439612286 1102 1 0.364090544956035 1102 2 0.32140946057939956 1102 3 0.37847447746260343 1102 4 0.34329276134827325 1102 5 0.43885119487236324 1102 6 0.40610866441269167 1102 7 0.41259390156245956 1102 8 0.39780372049263235 1102 9 0.34490217313354776 1102 10-11 0.40242486181556814 1102 12-13 0.3500148202653648 1102 14-15 0.3934416194278967 1102 16-17 0.5193199803114084 1102 18-19 0.43025124293353656 1102 20-21 0.4686444395301379 1102 22-23 0.5105051926308377 1102 24-25 0.5747233213287899 1102 26-27 0.6229188716538516 1102 28-29 0.6310652366732583 1102 30-31 0.5397536541599237 1102 32-33 0.523988749792025 1102 34-35 0.6088520698424915 1102 36-37 0.5538520560024125 1102 38-39 0.5752169976071357 1102 40-41 0.6302163342266169 1102 42-43 0.5842961533503086 1102 44-45 0.5690121512269357 1102 46-47 0.561199885729458 1102 48-49 0.5642911297781694 1102 50-51 0.64698899655383 1102 52-53 0.5410553589308051 1102 54-55 0.4780760713079033 1102 56-57 0.5381160861869105 1102 58-59 0.5137858652105649 1102 60-61 0.5718390368523671 1102 62-63 0.6058012582736509 1102 64-65 0.5354243078090448 1102 66-67 0.5666716537523016 1102 68-69 0.5595395278521522 1102 70-71 0.520198326656029 1102 72-73 0.5693496680454047 1102 74-75 0.6212806271362084 1102 76-77 0.5119031427405432 1102 78-79 0.5102333491773052 1102 80-81 0.5494081001457118 1102 82-83 0.47815992352027337 1102 84-85 0.44665133104140864 1102 86-87 0.43548536414201067 1102 88-89 0.4388544540642165 1102 90-91 0.4831178818461339 1102 92-93 0.38115723055198814 1102 94-95 0.40986978294183274 1102 96-97 0.3769903740055156 1102 98-99 0.44055913100838495 1102 100-101 0.46033814518947125 1102 102-103 0.5358982707372562 1102 104-105 0.5479896915526616 1102 106-107 0.5264252971283412 1102 108-109 0.4715943452728837 1102 110-111 0.4561622374374714 1102 112-113 0.4912468120293916 1102 114-115 0.5507670201410271 1102 116-117 0.5364239672381821 1102 118-119 0.5464193507143094 1102 120-121 0.5674171921894597 1102 122-123 0.5790278505841258 1102 124-125 0.7313719869706183 1103 1 0.17132916707952717 1103 2 0.17985137502460447 1103 3 0.20837486764259694 1103 4 0.1303103764086515 1103 5 0.1446444421952151 1103 6 0.11840040228008775 1103 7 0.0978300743808731 1103 8 0.1420766898478405 1103 9 0.20771866037437547 1103 10-11 0.11721289724504658 1103 12-13 0.20324484134078347 1103 14-15 0.27564286116860615 1103 16-17 0.2643104750140779 1103 18-19 0.25305808247557593 1103 20-21 0.24784136650666966 1103 22-23 0.28347401383156523 1103 24-25 0.25128849135612796 1103 26-27 0.2758601737727844 1103 28-29 0.25385427603301736 1103 30-31 0.2660940078851439 1103 32-33 0.007545119409243739 1103 34-35 0.16516828652054727 1103 36-37 0.216640774958897 1103 38-39 0.2476651634763627 1103 40-41 0.3235145656005045 1103 42-43 0.33677358271918933 1103 44-45 0.32161311386078495 1103 46-47 0.3215946581719322 1103 48-49 0.18808214139812662 1103 50-51 0.1679492451867759 1103 52-53 0.15201368514406965 1103 54-55 0.24581270693104074 1103 56-57 0.27295638295154845 1103 58-59 0.2886327836430098 1103 60-61 0.24702184472432265 1103 62-63 0.26997750143837607 1103 64-65 0.25043466751512966 1103 66-67 0.241252993465217 1103 68-69 0.24483906931945398 1103 70-71 0.23765371939925473 1103 72-73 0.24924204814337259 1103 74-75 0.24676532609952773 1103 76-77 0.18528902221194699 1103 78-79 0.22749115989729063 1103 80-81 0.17213572887663275 1103 82-83 0.17962096248131587 1103 84-85 0.16979477203024373 1103 86-87 0.199511907773811 1103 88-89 0.2138302458163217 1103 90-91 0.16926951803889523 1103 92-93 0.14603177486732122 1103 94-95 0.1639328525476671 1103 96-97 0.13865910549332483 1103 98-99 0.1791588262692656 1103 100-101 0.18648269690968178 1103 102-103 0.1986282490922342 1103 104-105 0.2484549883173024 1103 106-107 0.24201006871640374 1103 108-109 0.2132805921400518 1103 110-111 0.1306321480093544 1103 112-113 0.08535868973501692 1103 114-115 0.16831443982660588 1103 116-117 0.23737912501899316 1103 118-119 0.2649908148861009 1103 120-121 0.2973104622965437 1103 122-123 0.2780856869363717 1103 124-125 0.33926316377626975 1104 1 0.25013023481379193 1104 2 0.30064363264611416 1104 3 0.27327531216378276 1104 4 0.24726718316440355 1104 5 0.2535659362200775 1104 6 0.15916328616649622 1104 7 0.17387948770927864 1104 8 0.21682948266161617 1104 9 0.29361741033422106 1104 10-11 0.20238513414633275 1104 12-13 0.2682812121686311 1104 14-15 0.283879352864453 1104 16-17 0.3081252006316717 1104 18-19 0.40079102944625333 1104 20-21 0.28624842865129096 1104 22-23 0.2890772846963543 1104 24-25 0.300169031808764 1104 26-27 0.26197611469604 1104 28-29 0.36146998907391037 1104 30-31 0.31764757329039384 1104 32-33 -0.3097063030987073 1104 34-35 0.2181459159015091 1104 36-37 0.30106531678485027 1104 38-39 0.41781598746684523 1104 40-41 0.4019790300066859 1104 42-43 0.3804477390525136 1104 44-45 0.31697987395588 1104 46-47 0.3576177144458299 1104 48-49 0.35534759322762 1104 50-51 0.3411013651954562 1104 52-53 0.27700628147707107 1104 54-55 0.3334093059624976 1104 56-57 0.24039445315939645 1104 58-59 0.2974953996923517 1104 60-61 0.31030329550173263 1104 62-63 0.2808026915745856 1104 64-65 0.25078073247866683 1104 66-67 0.2693622324385174 1104 68-69 0.31030058631326085 1104 70-71 0.37544858612320553 1104 72-73 0.30018125977079535 1104 74-75 0.2301092588069693 1104 76-77 0.2746252320163123 1104 78-79 0.28497782325781884 1104 80-81 0.24795545284784737 1104 82-83 0.2418631642867055 1104 84-85 0.23490984760285727 1104 86-87 0.15676840493080135 1104 88-89 0.13193735654907712 1104 90-91 0.15412374861970335 1104 92-93 0.17038296266648345 1104 94-95 0.14880663382878367 1104 96-97 0.1332342504546169 1104 98-99 0.1371523271045021 1104 100-101 0.10349281006782718 1104 102-103 0.11515594432819043 1104 104-105 0.12095134420730957 1104 106-107 0.15201817008490082 1104 108-109 0.12150589049528548 1104 110-111 0.1271436907026633 1104 112-113 0.0944094091204768 1104 114-115 0.12706743141001553 1104 116-117 0.10148619760404642 1104 118-119 0.17451807945451137 1104 120-121 0.18719906095053318 1104 122-123 0.2425191984931203 1104 124-125 0.31438373061105196 1105 1 0.10326527858509493 1105 2 0.10568580298067687 1105 3 0.17080381577917336 1105 4 0.1766316057784607 1105 5 0.0703991895050109 1105 6 0.09799214744402462 1105 7 0.022156092478631706 1105 8 0.1059633599068519 1105 9 0.13973188854503604 1105 10-11 0.19527473455452338 1105 12-13 0.18147521110631715 1105 14-15 0.2615021104349111 1105 16-17 0.24341114381871165 1105 18-19 0.27336375341028685 1105 20-21 0.23347355551547366 1105 22-23 0.1993750116147055 1105 24-25 0.23257268598991487 1105 26-27 0.2031710111295979 1105 28-29 0.20820458785257756 1105 30-31 0.15076805684535088 1105 32-33 -0.19371263663386173 1105 34-35 0.2273788524898137 1105 36-37 0.2045049095955207 1105 38-39 0.1480604110386068 1105 40-41 0.16725830356951832 1105 42-43 0.14766719919494165 1105 44-45 0.062138907511581465 1105 46-47 0.09255379915934014 1105 48-49 0.05659507175251122 1105 50-51 0.20999427165494922 1105 52-53 0.21466419577078 1105 54-55 0.19308879741196705 1105 56-57 0.26868059916222364 1105 58-59 0.23186653589142736 1105 60-61 0.15087888600836408 1105 62-63 0.20630524430656294 1105 64-65 0.23719537805349233 1105 66-67 0.2497736946549054 1105 68-69 0.17395095554180529 1105 70-71 0.15646163085906295 1105 72-73 0.1001918799067667 1105 74-75 0.09798975038712854 1105 76-77 0.17623846444742952 1105 78-79 0.19886339770062733 1105 80-81 0.17159349616181174 1105 82-83 0.10781489412952538 1105 84-85 0.10049918641693978 1105 86-87 0.08380732574219252 1105 88-89 0.08448745336468377 1105 90-91 0.03928245135171693 1105 92-93 0.12598627406262963 1105 94-95 0.13033321032165546 1105 96-97 0.10154069492499218 1105 98-99 0.09116313967454914 1105 100-101 0.11030430101520494 1105 102-103 0.13834993879528668 1105 104-105 0.1240526424717423 1105 106-107 0.1874727588454661 1105 108-109 0.15770989923399625 1105 110-111 0.14043942537689347 1105 112-113 0.1324587037382976 1105 114-115 0.12991852562924677 1105 116-117 0.16928496182356767 1105 118-119 0.1779649295236716 1105 120-121 0.13667008827086136 1105 122-123 0.18820554812637624 1105 124-125 0.27502251639074515 1106 1 0.22033657589411604 1106 2 0.24101955240193718 1106 3 0.2619469746768992 1106 4 0.17638682560534846 1106 5 0.18207184666764675 1106 6 0.20154399216284702 1106 7 0.24992313234874075 1106 8 0.17691507999113298 1106 9 0.246133906792096 1106 10-11 0.2517812591642823 1106 12-13 0.2359175779886371 1106 14-15 0.3439260787109433 1106 16-17 0.2554297352181507 1106 18-19 0.321670693743485 1106 20-21 0.3883707032413639 1106 22-23 0.3524740274008238 1106 24-25 0.3805346512562622 1106 26-27 0.40639507390304885 1106 28-29 0.4422386523899462 1106 30-31 0.38096659255730714 1106 32-33 0.33370698708185387 1106 34-35 0.39366779802728047 1106 36-37 0.41572281468967986 1106 38-39 0.429330382755559 1106 40-41 0.4187239392435771 1106 42-43 0.41617205753465214 1106 44-45 0.43479293366105054 1106 46-47 0.3891020534172611 1106 48-49 0.4119830315414248 1106 50-51 0.3410078236713616 1106 52-53 0.372302099712023 1106 54-55 0.34953577195492613 1106 56-57 0.42690028764108945 1106 58-59 0.4824845530741868 1106 60-61 0.4246295167825025 1106 62-63 0.44986413029204897 1106 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2216 114-115 -0.7582759619591108 2216 116-117 -0.9167472390683038 2216 118-119 -0.9764402367011975 2216 120-121 -0.9178774097272608 2216 122-123 -1.0221003249683882 2216 124-125 -1.2250566526919826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49651.0 3 8620.0 4 4908.0 5 4170.0 6 4543.0 7 5578.0 8 6386.0 9 7127.0 10 8023.0 11 8754.0 12 9726.0 13 10094.0 14 9945.0 15 9247.0 16 8302.0 17 7510.0 18 7291.0 19 7597.0 20 8054.0 21 8534.0 22 10096.0 23 12903.0 24 16796.0 25 21345.0 26 26688.0 27 31968.0 28 38673.0 29 48002.0 30 56820.0 31 70331.0 32 90785.0 33 121129.0 34 169219.0 35 259058.0 36 502173.0 37 914798.0 38 388786.0 39 2651.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.959922166903645 17.727892038311598 12.201357353026566 25.110828441758187 2 16.19955192901076 19.462665870486084 38.614004429401476 25.72377777110168 3 19.16008133275547 23.31015808851823 29.25413920808575 28.275621370640547 4 12.73662665339942 15.223088253759093 36.31298511754882 35.727299975292674 5 13.748508847672586 37.828517651125374 34.35483315634163 14.06814034486041 6 34.29249831545207 35.3664883888629 16.587333888375692 13.753679407309333 7 29.721011525309244 31.863283657702535 20.448988896863256 17.96671592012496 8 27.432233013119202 34.28744558155496 19.58341200483817 18.69690940048767 9 26.866882129842683 15.36701927143361 19.23660775208043 38.52949084664327 10-11 25.44397969596654 25.725272555246292 27.63548621247746 21.1952615363097 12-13 25.846041047048594 23.106885014372462 27.568118216581173 23.478955721997767 14-15 22.654418180583647 25.01206820894267 24.600448542136714 27.73306506833697 16-17 23.24127278051964 28.206550358911514 25.71994261739401 22.832234243174838 18-19 23.416868527292102 27.066963564079693 26.74654272331365 22.769625185314556 20-21 23.507172198123367 26.481080068530417 26.90626187124923 23.10548586209698 22-23 23.3899445780104 26.494592943663093 26.812009178886935 23.303453299439564 24-25 23.125369823047432 26.725011786196124 26.930009673886808 23.21960871686964 26-27 22.693869842285032 27.08210330996859 26.623981371723747 23.600045476022633 28-29 23.00929167802496 27.41100753245095 26.508981414954985 23.0707193745691 30-31 22.933972519628597 26.82993032279336 26.448759586274274 23.787337571303773 32-33 22.603609603651662 27.053531931180686 26.53243300717708 23.81042545799057 34-35 23.533318447137848 27.04823971864415 26.310545405655024 23.10789642856298 36-37 23.731309192586156 26.57479859685895 26.215217959043933 23.478674251510956 38-39 23.063932408019163 26.80192825819691 26.421727585011023 23.7124117487729 40-41 23.45945774451677 26.66239372275513 26.625874565470127 23.252273967257977 42-43 23.119263670437398 26.66770973157619 26.423011429662235 23.790015168324178 44-45 23.108963209132995 26.93405278147286 26.781078905521966 23.175905103872182 46-47 23.664260138031473 27.148671210325066 26.009802675454875 23.177265976188583 48-49 23.192755473599213 27.152658091638376 26.056005263123243 23.598581171639164 50-51 23.334175598645558 27.20689124936978 26.286914724772508 23.17201842721216 52-53 23.638019003019988 26.729503697507244 26.342941518418673 23.2895357810541 54-55 22.95739650383086 27.054375308324435 26.533242669283304 23.454985518561404 56-57 23.110537837334803 27.48752699243714 26.21858503860064 23.183350131627417 58-59 23.07052397037285 26.79592567626638 26.337317847140625 23.796232506220143 60-61 22.726425822209038 27.08516234909249 26.671783691609463 23.516628137089015 62-63 22.92811375405951 27.128027331222277 26.872826769473836 23.07103214524438 64-65 22.679609815052064 27.19961784966165 26.524221140813285 23.596551194472998 66-67 22.656862323079118 27.32226462105655 26.454070414701114 23.56680264116322 68-69 23.1750477935938 26.678093685573188 26.819887537335052 23.326970983497958 70-71 22.950682405278137 26.780157221800373 26.48967537678907 23.779484996132425 72-73 22.857658048871002 26.966338612223506 26.456757071157217 23.719246267748275 74-75 22.768588114161503 27.388472665583187 26.3707302925313 23.47220892772402 76-77 23.152746947952842 26.959894808046464 26.51138732608476 23.37597091791594 78-79 23.158629608744246 27.036593066328095 26.541048593782186 23.26372873114547 80-81 23.306434458471625 27.13741385888558 26.335309669076313 23.220842013566486 82-83 23.146686565450736 27.157889760961357 26.47757757064884 23.217846102939067 84-85 23.00799350107041 27.29157075528954 26.425992206889344 23.27444353675071 86-87 23.17335970282332 27.20705777733964 26.34784364260878 23.27173887722826 88-89 22.97849748258064 27.432975028381634 26.142131851157846 23.44639563787988 90-91 23.033243702023466 27.283712211423445 26.329021454633285 23.354022631919808 92-93 23.032946236907602 26.852379780515957 26.631774989189182 23.48289899338726 94-95 22.84656774054171 26.644500553590234 26.99100078312765 23.51793092274041 96-97 23.188547651963535 26.311066903918434 26.783877230310623 23.716508213807405 98-99 22.829674857574428 26.782755182205708 26.481481512721427 23.906088447498437 100-101 22.830899023487053 26.51583189834333 26.88474016334595 23.768528914823673 102-103 22.61861328796724 26.079059726342745 27.150179205847124 24.15214777984289 104-105 22.39586179814627 26.6049094085189 26.776305535482592 24.22292325785224 106-107 22.811958332461828 26.501150721975993 26.351617589212157 24.335273356350022 108-109 22.805772726720626 26.395659320860442 26.497300149669282 24.301267802749653 110-111 22.80052524971635 26.58651467587665 26.73478534079473 23.87817473361227 112-113 22.785499395827845 26.759198966098698 26.827234774667662 23.6280668634058 114-115 22.734768792183772 26.96337923035676 26.507108622505505 23.794743354953965 116-117 22.724225093477386 26.795354626932355 26.715990377759653 23.76442990183061 118-119 22.54180901197726 27.039307560392672 26.377013651646703 24.041869775983365 120-121 22.625213907249783 27.261104484630156 26.455468050750724 23.658213557369336 122-123 22.58557609327429 27.190458635725957 26.439121753119394 23.784843517880358 124-125 22.617374141925662 27.147453208868576 26.486489405124143 23.748683244081622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12357.0 1 6536.0 2 742.0 3 696.0 4 592.5 5 546.5 6 485.0 7 456.5 8 478.5 9 467.5 10 434.5 11 406.0 12 406.5 13 445.0 14 483.0 15 543.5 16 620.5 17 733.5 18 912.5 19 1106.5 20 1468.5 21 2138.5 22 3180.5 23 4630.0 24 6503.0 25 9186.0 26 13028.5 27 17696.5 28 22093.0 29 26699.0 30 32511.0 31 39563.0 32 51511.0 33 56307.5 34 54166.5 35 56734.0 36 62551.5 37 68481.0 38 71079.0 39 74467.5 40 78402.0 41 82406.0 42 84456.0 43 86550.0 44 90459.5 45 97621.0 46 102574.0 47 104878.5 48 105937.5 49 104041.0 50 105057.0 51 105284.0 52 106954.0 53 113672.5 54 119439.0 55 115193.0 56 105905.0 57 98104.5 58 92164.5 59 82229.0 60 65494.0 61 53612.0 62 47249.5 63 36245.5 64 25048.0 65 18784.0 66 13406.0 67 10509.5 68 7931.5 69 5223.0 70 3916.0 71 2705.0 72 1938.0 73 1588.0 74 1242.5 75 909.0 76 626.0 77 377.5 78 244.0 79 161.5 80 105.5 81 74.5 82 59.0 83 40.5 84 32.0 85 28.0 86 21.5 87 20.5 88 17.5 89 16.0 90 12.0 91 8.5 92 9.5 93 5.5 94 2.5 95 4.0 96 3.5 97 2.5 98 4.0 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4718976467611762 2 0.46380029305028664 3 0.45818926371535484 4 0.4568453045932155 5 0.46390108998444707 6 0.5223297128194548 7 0.5176258558919672 8 0.5260927983614451 9 0.5321070154330185 10-11 0.5502336640928729 12-13 0.5587006065623508 14-15 0.5383900243290201 16-17 0.5309310512011467 18-19 0.5413299349086997 20-21 0.5421363103819834 22-23 0.5525351940895366 24-25 0.5460001928581341 26-27 0.5536943588323817 28-29 0.5338877612698533 30-31 0.5395659885608919 32-33 0.5407587522817906 34-35 0.5390620038900897 36-37 0.48710118433037736 38-39 0.4537038001452148 40-41 0.4519902522644871 42-43 0.465597838376148 44-45 0.47073848201833096 46-47 0.47477035938474893 48-49 0.48857953936473064 50-51 0.471444060557454 52-53 0.4708896774195716 54-55 0.46015480393148367 56-57 0.4429185281900466 58-59 0.46124677071822184 60-61 0.4576852790445526 62-63 0.4737791895321712 64-65 0.4736111946419038 66-67 0.4814229570393387 68-69 0.4901082928661642 70-71 0.4864460042583345 72-73 0.49832324300024095 74-75 0.48817635162808887 76-77 0.48575722520823805 78-79 0.49696248438907475 80-81 0.48957070921730844 82-83 0.49141865301025 84-85 0.4892851179038539 86-87 0.47952461477931685 88-89 0.4844468650641522 90-91 0.4690585331156567 92-93 0.4693777234071649 94-95 0.4636994961161261 96-97 0.4524102394901557 98-99 0.41543456414229707 100-101 0.41209146582597544 102-103 0.4015917851842618 104-105 0.4063796395568833 106-107 0.4050356804347439 108-109 0.417064114577891 110-111 0.4407849930836504 112-113 0.4413393762215329 114-115 0.43369560871436535 116-117 0.4277149906208453 118-119 0.47041929172682284 120-121 0.46502665574923874 122-123 0.428840556385637 124-125 0.4378786814820241 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2976281.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.06136542031521 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26309350399086 52.87087821648393 2 10.750673078346292 13.989069393292661 3 3.555909807005211 6.9405704206574566 4 1.6032825860402173 4.1724701680956215 5 0.8204464799792557 2.668968412087084 6 0.4997780977340808 1.950974726744822 7 0.3243963242910734 1.4773967456996022 8 0.21864962327712345 1.138051443123775 9 0.15243307060529648 0.8925753337912713 >10 0.7420158423953638 8.473063554319237 >50 0.05006107517115902 2.199050433400231 >100 0.01887009742711354 2.083384849153478 >500 2.602758246146777E-4 0.10346613227086277 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 1.3013791230733886E-4 1.0400801708799332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 12284 0.41272984640899163 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0159386832090115E-4 0.0 12-13 0.0 0.0 0.0 2.0159386832090115E-4 0.0 14-15 0.0 0.0 0.0 2.183933573476429E-4 0.0 16-17 0.0 0.0 0.0 2.687918244278682E-4 0.0 18-19 0.0 0.0 0.0 3.52789269561577E-4 0.0 20-21 0.0 0.0 0.0 4.199872256685441E-4 0.0 22-23 0.0 0.0 0.0 5.039846708022529E-4 0.0 24-25 0.0 0.0 0.0 5.879821159359617E-4 0.0 26-27 0.0 0.0 0.0 8.231749623103464E-4 0.0 28-29 0.0 0.0 0.0 0.0023687279527705883 0.0 30-31 0.0 0.0 0.0 0.007324577215659408 0.0 32-33 0.0 0.0 0.0 0.018479437929415938 0.0 34-35 0.0 0.0 0.0 0.03663968556732378 0.0 36-37 0.0 0.0 0.0 0.06682836734837873 0.0 38-39 0.0 0.0 0.0 0.1118677974290734 0.0 40-41 0.0 0.0 0.0 0.1619470742177906 0.0 42-43 0.0 0.0 0.0 0.21337031012864713 0.0 44-45 0.0 0.0 0.0 0.2802994744111863 0.0 46-47 0.0 0.0 0.0 0.3602314432004236 0.0 48-49 0.0 0.0 0.0 0.445623245923352 0.0 50-51 0.0 0.0 0.0 0.5451266194287434 0.0 52-53 0.0 0.0 0.0 0.6401613288530216 0.0 54-55 0.0 0.0 0.0 0.7459813102324679 0.0 56-57 0.0 0.0 0.0 0.8721958712903788 0.0 58-59 0.0 0.0 0.0 1.0140675561212131 0.0 60-61 0.0 0.0 0.0 1.1774425869062766 0.0 62-63 0.0 0.0 0.0 1.3380457020019279 0.0 64-65 0.0 0.0 0.0 1.4997239843952905 0.0 66-67 0.0 0.0 0.0 1.6984787390706724 0.0 68-69 0.0 0.0 0.0 1.926195812828157 0.0 70-71 0.0 0.0 0.0 2.1805400766930276 0.0 72-73 0.0 0.0 0.0 2.4459216048484667 0.0 74-75 0.0 0.0 0.0 2.7023994038197334 0.0 76-77 0.0 0.0 0.0 2.995046502665575 0.0 78-79 0.0 0.0 0.0 3.3375376854537593 0.0 80-81 0.0 0.0 0.0 3.7196924618340805 0.0 82-83 0.0 0.0 0.0 4.118613128263091 0.0 84-85 0.0 0.0 0.0 4.512880336231693 0.0 86-87 0.0 0.0 0.0 4.931036417596323 0.0 88-89 0.0 0.0 0.0 5.385832184528276 0.0 90-91 0.0 0.0 0.0 5.904130019981312 0.0 92-93 0.0 0.0 0.0 6.4323731529381805 0.0 94-95 0.0 0.0 0.0 6.9618090496159475 0.0 96-97 0.0 0.0 0.0 7.4988551148228275 0.0 98-99 0.0 0.0 0.0 8.070760119760196 0.0 100-101 0.0 0.0 0.0 8.716465279992043 0.0 102-103 0.0 0.0 0.0 9.395147837183384 0.0 104-105 0.0 0.0 0.0 10.066253824823665 0.0 106-107 0.0 0.0 0.0 10.720056338766401 0.0 108-109 0.0 0.0 0.0 11.42240937599642 0.0 110-111 0.0 3.3598978053483525E-5 0.0 12.17699202461058 0.0 112-113 0.0 3.3598978053483525E-5 0.0 12.969087932221454 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2525 0.0 53.976433 1 GTATCAA 4110 0.0 47.496555 1 ATCAACG 4760 0.0 40.390976 3 TCAACGC 4940 0.0 39.045013 4 CAACGCA 4955 0.0 38.929443 5 GTACATG 10340 0.0 37.758385 1 TACATGG 10115 0.0 37.540455 2 AACGCAG 5185 0.0 37.08777 6 TATCAAC 5400 0.0 35.710506 2 ACATGGG 10600 0.0 34.59104 3 ACGCAGA 6255 0.0 29.982979 7 CGCAGAG 6355 0.0 29.04275 8 CATGGGA 4900 0.0 27.214443 4 CATGGGG 6405 0.0 27.140089 4 GAGTACA 7290 0.0 26.69625 1 TAAGGTG 1745 0.0 25.929554 5 GTATAGG 800 0.0 24.550142 1 GCAGAGT 7900 0.0 22.30777 9 GTAAGGT 1820 0.0 22.242573 4 GGTAAGG 1900 0.0 21.616446 3 >>END_MODULE