FastQCFastQC Report
Thu 26 May 2016
SRR1364129_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364129_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976281
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71450.24006469819213977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68210.22917862930281113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38810.13039763382556957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46500.073.4431461
GTATCAA84550.054.7717971
ATCAACG110300.041.694573
TCAACGC112400.040.915584
TATCAAC113900.040.4825742
CAACGCA114350.040.217855
AACGCAG117650.039.140346
GTGGTAT21200.037.6719861
TGGTATC19750.037.65592
GTACATG98950.033.6701931
ACGCAGA137700.033.229697
TACATGG98850.033.043482
CGCAGAG139800.032.602848
ACATGGG98800.030.95153
TAAGGTG18450.029.9890085
GCAGAGT160550.028.2779649
GTAAGGT18350.026.9102384
GAGTACT113900.026.09430112-13
AGAGTAC144800.025.74461610-11
CAGAGTA151100.025.5375610-11
Produced by FastQC (version 0.11.5)