##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364129_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2976281 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.136256623618536 33.0 31.0 34.0 30.0 34.0 2 32.17077554169112 34.0 31.0 34.0 30.0 34.0 3 32.30470510008968 34.0 31.0 34.0 30.0 34.0 4 35.923448424392724 37.0 35.0 37.0 35.0 37.0 5 35.85288519464392 37.0 35.0 37.0 35.0 37.0 6 35.88086507960774 37.0 35.0 37.0 35.0 37.0 7 35.80206002054241 37.0 35.0 37.0 33.0 37.0 8 35.781257213280604 37.0 35.0 37.0 33.0 37.0 9 37.44933794893694 39.0 37.0 39.0 34.0 39.0 10-11 37.36663809633566 39.0 37.0 39.0 34.0 39.0 12-13 37.31311341234245 39.0 37.0 39.0 33.5 39.0 14-15 38.65195473814468 40.0 38.0 41.0 34.0 41.0 16-17 38.6306543300179 40.0 38.0 41.0 34.0 41.0 18-19 38.75300971245659 40.0 38.0 41.0 34.0 41.0 20-21 38.79896656263303 40.0 38.0 41.0 34.0 41.0 22-23 38.77787984400666 40.0 38.0 41.0 34.0 41.0 24-25 38.74572965388685 40.0 38.0 41.0 34.0 41.0 26-27 38.630382682280334 40.0 38.0 41.0 34.0 41.0 28-29 38.55740418999416 40.0 38.0 41.0 34.0 41.0 30-31 38.420045183905685 40.0 38.0 41.0 34.0 41.0 32-33 38.28630865163605 40.0 38.0 41.0 34.0 41.0 34-35 38.1718649885545 40.0 38.0 41.0 33.0 41.0 36-37 38.052451364639296 40.0 37.0 41.0 33.0 41.0 38-39 37.93283245096817 40.0 37.0 41.0 33.0 41.0 40-41 37.7924717121804 40.0 37.0 41.0 33.0 41.0 42-43 37.643005482345245 40.0 37.0 41.0 33.0 41.0 44-45 37.489640427096774 40.0 37.0 41.0 32.0 41.0 46-47 37.24262712425339 40.0 36.5 41.0 31.5 41.0 48-49 36.98087361374816 40.0 36.0 41.0 31.0 41.0 50-51 36.792773095013544 40.0 36.0 41.0 31.0 41.0 52-53 36.61514621771264 39.5 35.5 41.0 30.5 41.0 54-55 36.42501413677002 39.0 35.0 41.0 30.0 41.0 56-57 36.2147122533121 39.0 35.0 41.0 30.0 41.0 58-59 35.98002742348589 39.0 35.0 41.0 29.5 41.0 60-61 35.72280876704854 38.0 35.0 40.5 29.0 41.0 62-63 35.445099101865715 37.5 35.0 40.0 29.0 41.0 64-65 35.21271949792376 37.0 34.0 40.0 29.0 41.0 66-67 35.00506151804886 37.0 34.0 39.5 29.0 41.0 68-69 34.69215826731414 36.0 34.0 39.0 29.0 41.0 70-71 34.36562105526998 36.0 34.0 39.0 29.0 41.0 72-73 34.05049859203483 35.0 34.0 38.5 28.0 40.5 74-75 33.7945362013869 35.0 34.0 37.0 28.5 39.5 76-77 33.618859408772224 35.0 34.0 37.0 29.0 39.0 78-79 33.35562535929907 35.0 34.0 36.5 29.0 39.0 80-81 33.12146769743852 35.0 34.0 36.0 29.0 38.0 82-83 32.88133445733114 35.0 34.0 36.0 28.5 37.0 84-85 32.69244923446408 35.0 34.0 35.0 28.0 37.0 86-87 32.533450470570486 35.0 33.0 35.0 28.0 36.5 88-89 32.40010049454335 35.0 33.0 35.0 27.0 36.0 90-91 32.28503138648534 35.0 33.0 35.0 27.0 36.0 92-93 32.186513471006265 35.0 33.0 35.0 27.0 36.0 94-95 32.107546800856504 35.0 33.0 35.0 27.0 35.5 96-97 32.052257666530814 35.0 33.0 35.0 27.0 35.0 98-99 31.9941484019822 35.0 33.0 35.0 27.0 35.0 100-101 31.934209673078584 35.0 33.0 35.0 27.0 35.0 102-103 30.731890570816397 34.0 31.0 35.0 24.0 35.0 104-105 31.683479315293148 35.0 33.0 35.0 25.0 35.0 106-107 31.838387571603622 35.0 33.0 35.0 26.5 35.0 108-109 31.840300025434427 35.0 33.0 35.0 27.0 35.0 110-111 31.795756348274914 35.0 33.0 35.0 25.5 35.0 112-113 31.740694175045974 35.0 33.0 35.0 25.0 35.0 114-115 31.67081082061808 35.0 33.0 35.0 25.0 35.0 116-117 31.608012314697437 35.0 33.0 35.0 25.0 35.0 118-119 31.517492635943988 35.0 33.0 35.0 25.0 35.0 120-121 31.404766048635864 35.0 33.0 35.0 25.0 35.0 122-123 31.275290874752752 35.0 33.0 35.0 24.5 35.0 124-125 30.429398467416213 34.5 31.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20838570657830502 1101 2 0.27676543161096134 1101 3 0.22007529394770842 1101 4 0.1844391178338114 1101 5 0.15359544633094657 1101 6 0.20964662782060373 1101 7 0.2160840725590063 1101 8 0.1799343968337439 1101 9 0.28184741440279026 1101 10-11 0.26143307879793554 1101 12-13 0.24126460476307443 1101 14-15 0.326308323173194 1101 16-17 0.3182084169620296 1101 18-19 0.3528585872235013 1101 20-21 0.3159989109388164 1101 22-23 0.3253442058033329 1101 24-25 0.3366530841267874 1101 26-27 0.326528986583547 1101 28-29 0.41764053879700924 1101 30-31 0.43615349992914787 1101 32-33 0.3816736572437236 1101 34-35 0.4409880439731779 1101 36-37 0.4742385971632288 1101 38-39 0.4511046628532469 1101 40-41 0.4595864967510863 1101 42-43 0.44276356808869366 1101 44-45 0.42887427580087945 1101 46-47 0.42597552440525277 1101 48-49 0.44426470315145394 1101 50-51 0.4540540352098077 1101 52-53 0.4384274132087569 1101 54-55 0.47438351476331775 1101 56-57 0.4820279204530422 1101 58-59 0.458632073686438 1101 60-61 0.4090468240873122 1101 62-63 0.44921742145005794 1101 64-65 0.42825108988238725 1101 66-67 0.43734262954448155 1101 68-69 0.3999702941982335 1101 70-71 0.4769360915214662 1101 72-73 0.3952378619474999 1101 74-75 0.35949762928892426 1101 76-77 0.3655807230196686 1101 78-79 0.38861017384038377 1101 80-81 0.3561514533745651 1101 82-83 0.3659872670511817 1101 84-85 0.31499939669799915 1101 86-87 0.36225485125788737 1101 88-89 0.3124058627395172 1101 90-91 0.3648685237097098 1101 92-93 0.3483530617862911 1101 94-95 0.3427727291976126 1101 96-97 0.36643483500078844 1101 98-99 0.36607448704776147 1101 100-101 0.3496620422567318 1101 102-103 0.3903931379197729 1101 104-105 0.34134241690544087 1101 106-107 0.3343944582900207 1101 108-109 0.3036197787727275 1101 110-111 0.30230157676338365 1101 112-113 0.31179274154470704 1101 114-115 0.3578549439708354 1101 116-117 0.3786657530894928 1101 118-119 0.33413004854109474 1101 120-121 0.35775613906387704 1101 122-123 0.39320855173824754 1101 124-125 0.5289328936205564 1102 1 0.2706383380334856 1102 2 0.2975701492778029 1102 3 0.21970908561647917 1102 4 0.16515322872113103 1102 5 0.17118716421572344 1102 6 0.14462925497162615 1102 7 0.12667364579660045 1102 8 0.17057692562165983 1102 9 0.263460620635378 1102 10-11 0.21476696703926734 1102 12-13 0.30203167120072294 1102 14-15 0.3076255097154217 1102 16-17 0.3166270065447563 1102 18-19 0.3504240573414492 1102 20-21 0.36797880821070095 1102 22-23 0.35932182547702496 1102 24-25 0.3497826403932791 1102 26-27 0.37928846253998927 1102 28-29 0.3436309232110659 1102 30-31 0.42812997389318497 1102 32-33 0.4059150913157552 1102 34-35 0.393697480224823 1102 36-37 0.43077328855651587 1102 38-39 0.33475654741051386 1102 40-41 0.35452013522193226 1102 42-43 0.3908304202679176 1102 44-45 0.3768806347576543 1102 46-47 0.3206195380361905 1102 48-49 0.3179343808644006 1102 50-51 0.27293046823648837 1102 52-53 0.28119423396037746 1102 54-55 0.3059253101134658 1102 56-57 0.23216553866624423 1102 58-59 0.2510734584356058 1102 60-61 0.2939542137598963 1102 62-63 0.28879826581992774 1102 64-65 0.2383007984989618 1102 66-67 0.2709771139199475 1102 68-69 0.28765668474969885 1102 70-71 0.2023687005760948 1102 72-73 0.21237770629065267 1102 74-75 0.24672356482201963 1102 76-77 0.2715860394095415 1102 78-79 0.23936658666345778 1102 80-81 0.2285697258964845 1102 82-83 0.24044676036655233 1102 84-85 0.2191862032757399 1102 86-87 0.20200072402656133 1102 88-89 0.15207953558370946 1102 90-91 0.15363243109123204 1102 92-93 0.16609438307173008 1102 94-95 0.22901486340263233 1102 96-97 0.2516873403123867 1102 98-99 0.22387500814556205 1102 100-101 0.2437012525917268 1102 102-103 0.33244284839291893 1102 104-105 0.31500293038511984 1102 106-107 0.26461952298888036 1102 108-109 0.3193161454971829 1102 110-111 0.28065799911685474 1102 112-113 0.25104480808704466 1102 114-115 0.28265748491308784 1102 116-117 0.2615031967129582 1102 118-119 0.24607254522067024 1102 120-121 0.224560970600308 1102 122-123 0.31331407502215924 1102 124-125 0.46613492066438056 1103 1 0.17046959230552972 1103 2 0.21527883861477903 1103 3 0.16451534843260873 1103 4 0.1085080646746519 1103 5 0.11845056419294053 1103 6 0.12959272899760066 1103 7 0.15576377167948863 1103 8 0.1849092763849356 1103 9 0.2451200248276777 1103 10-11 0.2468409374767191 1103 12-13 0.1910081963203396 1103 14-15 0.15824282003667633 1103 16-17 0.2657709957791994 1103 18-19 0.20172311464379078 1103 20-21 0.296069987864378 1103 22-23 0.3421551516902923 1103 24-25 0.2841724145445639 1103 26-27 0.3069992522984819 1103 28-29 0.31857841267336084 1103 30-31 0.40689969649514524 1103 32-33 0.3739648321451057 1103 34-35 0.41087038407535204 1103 36-37 0.34630750762691065 1103 38-39 0.3829505409170153 1103 40-41 0.42737396724517396 1103 42-43 0.3867248082264467 1103 44-45 0.3359355874803782 1103 46-47 0.37386468926653293 1103 48-49 0.3189587813542616 1103 50-51 0.23929097176804248 1103 52-53 0.34667078044020627 1103 54-55 0.4198192423873337 1103 56-57 0.354330503635488 1103 58-59 0.35507938945633555 1103 60-61 0.3289534661522424 1103 62-63 0.37366277083245336 1103 64-65 0.3796395858081425 1103 66-67 0.29348526640342953 1103 68-69 0.35431569733798796 1103 70-71 0.2785465386371584 1103 72-73 0.3838080577374541 1103 74-75 0.30821109045427875 1103 76-77 0.2349091198378872 1103 78-79 0.20725523584322758 1103 80-81 0.15257451770554553 1103 82-83 0.14298720238067375 1103 84-85 0.1456874706487099 1103 86-87 0.07611747898212684 1103 88-89 0.022109368689200437 1103 90-91 -0.006643614135477094 1103 92-93 0.05654939133101067 1103 94-95 0.04762619998270878 1103 96-97 0.029503329148113266 1103 98-99 0.008758737354952473 1103 100-101 0.033470063824349694 1103 102-103 0.15036709793723801 1103 104-105 0.14816929800872458 1103 106-107 0.20242105807660238 1103 108-109 0.15197057987858997 1103 110-111 0.10064368777030452 1103 112-113 0.18143256725769774 1103 114-115 0.20795563796617955 1103 116-117 0.23524703784988787 1103 118-119 0.16583056954284103 1103 120-121 0.2896973555968927 1103 122-123 0.24199800074556066 1103 124-125 0.4292289127468578 1104 1 0.2112580251173739 1104 2 0.21598020430272413 1104 3 0.11879516990133965 1104 4 0.12004644126882624 1104 5 0.1146455812991718 1104 6 0.1283246690695634 1104 7 0.127355123393329 1104 8 0.15498947692140774 1104 9 0.1387621069697289 1104 10-11 0.13040487203132045 1104 12-13 0.15082011306196108 1104 14-15 0.17125972147906765 1104 16-17 0.21239344289443096 1104 18-19 0.2056099143321859 1104 20-21 0.18711657023130357 1104 22-23 0.27370764795583113 1104 24-25 0.2311604773236482 1104 26-27 0.24488712915767508 1104 28-29 0.26435252503642914 1104 30-31 0.21571618393451075 1104 32-33 0.26319026301342774 1104 34-35 0.17621590192045744 1104 36-37 0.1860149046682622 1104 38-39 0.16566546309568508 1104 40-41 0.22845005887194958 1104 42-43 0.26689997145847855 1104 44-45 0.2982693939791403 1104 46-47 0.2635980618853111 1104 48-49 0.24052370574148796 1104 50-51 0.27369679110049816 1104 52-53 0.2712577311132165 1104 54-55 0.23936284019805498 1104 56-57 0.24909470562558766 1104 58-59 0.27108423521912783 1104 60-61 0.25785309049770433 1104 62-63 0.28789061102969526 1104 64-65 0.30202932889336864 1104 66-67 0.2884058739960338 1104 68-69 0.26829600408571963 1104 70-71 0.14852958800430827 1104 72-73 0.16599864970957157 1104 74-75 0.14361397040545398 1104 76-77 0.10260403454763889 1104 78-79 0.16633225706942056 1104 80-81 0.13025898697054572 1104 82-83 0.11446877904552366 1104 84-85 0.15039946549275385 1104 86-87 0.10519511759885347 1104 88-89 0.11444594591622348 1104 90-91 0.1387869429816888 1104 92-93 0.09018650925159477 1104 94-95 0.1063848886251364 1104 96-97 0.07368168775262518 1104 98-99 0.10761114387299386 1104 100-101 0.0926487537115932 1104 102-103 0.06946752980944382 1104 104-105 0.15494799512395474 1104 106-107 0.07798388735155726 1104 108-109 0.0034418265456857 1104 110-111 0.018063587622531685 1104 112-113 0.06873510267483596 1104 114-115 0.11837701453625726 1104 116-117 0.048831361350483604 1104 118-119 0.09632577196662595 1104 120-121 0.05094111643887089 1104 122-123 0.09652693678590651 1104 124-125 0.1610714821756858 1105 1 0.12125545254094305 1105 2 0.1694019669085094 1105 3 0.10722895765032092 1105 4 -0.00765438522909534 1105 5 0.0557846450254047 1105 6 0.11644626058585317 1105 7 0.12282780612361677 1105 8 0.07965198087229197 1105 9 0.08501507910519024 1105 10-11 0.1249539804490496 1105 12-13 0.13061413706476088 1105 14-15 0.20754867143622135 1105 16-17 0.14710513873890818 1105 18-19 0.19227558356045193 1105 20-21 0.13460272990420208 1105 22-23 0.11687146196356935 1105 24-25 0.18857075170414106 1105 26-27 0.13501762519601357 1105 28-29 0.13527552661668096 1105 30-31 0.22640404934628577 1105 32-33 0.19716289574760992 1105 34-35 0.24443062974420116 1105 36-37 0.2265854136037504 1105 38-39 0.24522160698094098 1105 40-41 0.22420836265840194 1105 42-43 0.28360259409062394 1105 44-45 0.2816222315051107 1105 46-47 0.2308086652913417 1105 48-49 0.26500731653208476 1105 50-51 0.2772542634465438 1105 52-53 0.23848157243428147 1105 54-55 0.32779236101681875 1105 56-57 0.31627084077928913 1105 58-59 0.30525539197298457 1105 60-61 0.2774183563743975 1105 62-63 0.26293423529961757 1105 64-65 0.2458848227200079 1105 66-67 0.32069332262956607 1105 68-69 0.2585996907978654 1105 70-71 0.2671550413904811 1105 72-73 0.2553071879561415 1105 74-75 0.2596692068353619 1105 76-77 0.25660355639315213 1105 78-79 0.222810194780088 1105 80-81 0.22343987615012395 1105 82-83 0.2561573876184582 1105 84-85 0.19576177679596185 1105 86-87 0.2403710941956092 1105 88-89 0.2570085549210148 1105 90-91 0.22813762896149825 1105 92-93 0.2412160688452829 1105 94-95 0.23228528416807848 1105 96-97 0.23842371555980435 1105 98-99 0.22946786552245868 1105 100-101 0.26710944775883405 1105 102-103 0.29181964752068623 1105 104-105 0.26118608814094557 1105 106-107 0.2615510499507536 1105 108-109 0.2745706441753484 1105 110-111 0.27694268939547584 1105 112-113 0.31133854803967154 1105 114-115 0.3263545944258297 1105 116-117 0.24521118648979723 1105 118-119 0.262001879973905 1105 120-121 0.24571346627409696 1105 122-123 0.30637779341809335 1105 124-125 0.24559547034570883 1106 1 0.12408308557517245 1106 2 0.19463389180626933 1106 3 0.08138939858458372 1106 4 0.09330913935984597 1106 5 0.07839750839615078 1106 6 0.10367476872838921 1106 7 0.07805874019881287 1106 8 0.16819670039708967 1106 9 0.10076076516636334 1106 10-11 0.1599627487012114 1106 12-13 0.19587514975064835 1106 14-15 0.16135696463069138 1106 16-17 0.2473965249802461 1106 18-19 0.2373593771275111 1106 20-21 0.2570332694536077 1106 22-23 0.20598324276714663 1106 24-25 0.25045654122714467 1106 26-27 0.25229648688380735 1106 28-29 0.26319582745842496 1106 30-31 0.2906009828607523 1106 32-33 0.23643294439000329 1106 34-35 0.33198821950740154 1106 36-37 0.24747378530820185 1106 38-39 0.3079634317116202 1106 40-41 0.3513627183307122 1106 42-43 0.3603987525382095 1106 44-45 0.32803217345518476 1106 46-47 0.2825201793881149 1106 48-49 0.3192081164606506 1106 50-51 0.3462737116725165 1106 52-53 0.4120834215604887 1106 54-55 0.41691915283023206 1106 56-57 0.44714049332284844 1106 58-59 0.453056552395978 1106 60-61 0.4262514877544987 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-1.006601657561589 2216 76-77 -0.831298717463703 2216 78-79 -0.7464681024504287 2216 80-81 -0.7724316826912769 2216 82-83 -0.723781770833611 2216 84-85 -0.6896919936369983 2216 86-87 -0.7508728483988243 2216 88-89 -0.7854582156361936 2216 90-91 -0.7372859752465857 2216 92-93 -0.7157720372404199 2216 94-95 -0.768032902199824 2216 96-97 -0.8387661400979205 2216 98-99 -0.8982105086767938 2216 100-101 -0.9193883191121586 2216 102-103 -0.9539979814278432 2216 104-105 -0.9003829093325493 2216 106-107 -0.9776601671218117 2216 108-109 -0.9212654072245812 2216 110-111 -1.0094815602455682 2216 112-113 -0.9482549327423015 2216 114-115 -1.0030996695735048 2216 116-117 -1.0048013252453458 2216 118-119 -1.1177786814492237 2216 120-121 -1.251575585871361 2216 122-123 -1.3195750399290453 2216 124-125 -1.6925158789198065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19.0 3 17.0 4 43.0 5 171.0 6 464.0 7 1133.0 8 2175.0 9 3588.0 10 6068.0 11 8393.0 12 10880.0 13 14318.0 14 20292.0 15 19263.0 16 9711.0 17 7894.0 18 7536.0 19 7311.0 20 7007.0 21 7325.0 22 7913.0 23 9396.0 24 12279.0 25 16382.0 26 21587.0 27 28166.0 28 36441.0 29 47427.0 30 60048.0 31 76356.0 32 97278.0 33 126378.0 34 172291.0 35 255188.0 36 499097.0 37 959092.0 38 414542.0 39 2812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.894565161054075 18.333435423214162 12.968096126934295 24.803903288797464 2 16.55171649110263 20.04534615587506 37.730615744752484 25.672321608269826 3 19.278690419352206 23.627876534507326 29.822822509030566 27.270610537109903 4 13.163172428947401 15.852333835414061 35.792655330595466 35.19183840504307 5 13.263868566173691 37.81948008269381 34.43962448438168 14.477026866750823 6 33.842436248459066 36.0138710020996 16.02889646508512 14.114796284356215 7 28.809040544222807 32.48419756064699 20.523129368497127 18.18363252663307 8 27.298598485828457 34.18121474417234 19.28426784970908 19.23591892029012 9 26.63207985541678 14.850489487040697 19.42868931306507 39.08874134447745 10-11 25.35023727701941 25.690092424472528 27.469787666970852 21.489882631537213 12-13 26.078256745389123 23.077069112696076 27.847122153793478 22.99755198812132 14-15 22.682888477264076 24.641876892672432 25.55407234733548 27.121162282728008 16-17 22.554204253619176 27.947960287817065 26.576106869498034 22.921728589065726 18-19 22.71467311050267 26.015218321119544 28.801631969562013 22.46847659881577 20-21 22.800048113736572 25.40554806485006 29.275377560116134 22.51902626129724 22-23 22.64619839322967 25.41070550798127 29.164568130495745 22.778527968293318 24-25 22.508089793940826 25.614029723671926 29.24522247731313 22.63265800507412 26-27 22.095763135268477 25.936210324226778 29.00290664759141 22.96511989291334 28-29 22.344580367243548 26.283690955255906 28.913281373633737 22.458447303866805 30-31 22.303976002265916 25.685561947947793 28.92025316157984 23.09020888820646 32-33 22.102415732923067 25.837681321084936 28.93246639010228 23.127436555889716 34-35 22.912083906055912 25.894597989907535 28.630949161050317 22.56236894298623 36-37 23.168208915757617 25.45262023310299 28.4588719949494 22.92029885618999 38-39 22.62207432698727 25.72856863985625 28.58184425462515 23.06751277853133 40-41 22.98112980595582 25.624865394094172 28.636476192940112 22.75752860700989 42-43 22.77048101304951 25.64158088567578 28.338604453006955 23.249333648267754 44-45 22.768582670789485 25.93315281722391 28.529446648350742 22.76881786363586 46-47 23.42616506976324 26.129740437814842 27.602484442833187 22.84161004958873 48-49 23.17128322224951 26.347529013557526 27.34064760686239 23.140540157330573 50-51 23.323486592831795 26.50707376084449 27.291509101459173 22.877930544864547 52-53 23.57079187079446 26.08577617503186 27.377539284765113 22.965892669408568 54-55 23.000953203007377 26.3839503057675 27.426073008563375 23.189023482661753 56-57 23.129435695083895 26.737781143648736 27.14162406036258 22.991159100904788 58-59 22.983448135441513 26.141180889842055 27.323125739807498 23.552245234908938 60-61 22.71778101597262 26.383295125695454 27.610161809318406 23.288762049013517 62-63 22.869178011081615 26.338910875686807 27.8741321803956 22.91777893283598 64-65 22.54049600827341 26.341128408238333 27.66376897880274 23.454606604685512 66-67 22.47983305339785 26.527518738990036 27.53211138330017 23.46053682431195 68-69 22.95480500665092 25.942006147941004 27.829277544694204 23.273911300713877 70-71 22.749649646656348 26.113310537546692 27.449693090134968 23.687346725661993 72-73 22.649020707386164 26.297231343411458 27.40616561406668 23.647582335135695 74-75 22.62027678166141 26.68603871744637 27.148696645242836 23.54498785564938 76-77 22.9269010553775 26.37966643606568 27.280253443811254 23.413179064745567 78-79 22.954082628622768 26.44536923764927 27.196356795611702 23.40419133811626 80-81 23.10163254074464 26.62473738198779 26.843987513275795 23.429642563991774 82-83 22.95871928759415 26.599386953046437 27.032763371469294 23.409130387890123 84-85 22.803324014096788 26.635405057519772 26.963583075657173 23.59768785272627 86-87 22.957711318252542 26.54114312459072 26.840325224667964 23.660820332488765 88-89 22.753580727088604 26.825306481478062 26.63407789788666 23.787034893546675 90-91 22.674740725086107 26.67837815045018 26.74386255867642 23.903018565787303 92-93 22.669066306082374 26.264308757188715 27.03849653955667 24.028128397172242 94-95 22.473278995626565 26.081813656620124 27.249485806616246 24.195421541137065 96-97 22.759819898524917 25.714408118006787 27.13421928026612 24.391552703202176 98-99 22.466596454527785 26.078008049476203 26.844517632021976 24.610877863974032 100-101 22.346945924110397 25.81473187848108 27.176226157134053 24.662096040274466 102-103 22.105526478220447 25.40904177627368 27.40513540421005 25.08029634129582 104-105 21.883996633668122 25.870737627562658 27.063571016816706 25.181694721952514 106-107 22.266698053464083 25.76874550432014 26.688103109998124 25.276453332217656 108-109 22.153558785627304 25.586207835238223 26.88104647737582 25.379186901758654 110-111 22.13978702573135 25.711206595995606 27.031286150926192 25.117720227346847 112-113 21.993124376067964 25.94871696636409 27.063993645618194 24.994165011949754 114-115 21.844163744183742 26.05179978457366 26.78001475384768 25.324021717394924 116-117 21.831914735233017 25.888349694836034 27.01319075264369 25.26654481728726 118-119 21.549481070019002 26.099094875891975 26.64949501568467 25.701929038404348 120-121 21.56351039493808 26.2909782832616 26.778773981048055 25.366737340752266 122-123 21.42309641326731 26.213482776048302 26.70272015850229 25.660700652182094 124-125 21.2691439097118 26.172686546374923 26.779874714724222 25.77829482918905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 359.0 1 268.5 2 241.5 3 327.5 4 237.5 5 168.0 6 440.5 7 977.5 8 1503.0 9 1724.0 10 1419.0 11 1104.0 12 1134.0 13 1284.5 14 1534.0 15 1784.5 16 2036.5 17 2303.5 18 2607.5 19 2932.5 20 3450.5 21 4269.0 22 5331.0 23 6820.0 24 8745.5 25 11420.5 26 15291.0 27 19548.0 28 23718.0 29 28807.5 30 34351.5 31 41037.5 32 53336.5 33 57883.5 34 54985.0 35 56705.5 36 62418.5 37 68122.0 38 70519.0 39 73993.5 40 77545.5 41 80828.0 42 82754.5 43 84865.0 44 88572.5 45 95973.0 46 100885.0 47 103492.0 48 104355.5 49 102946.5 50 104301.0 51 104196.5 52 106937.0 53 113692.5 54 118904.0 55 114527.0 56 105300.0 57 97602.0 58 91001.5 59 81372.5 60 65477.5 61 53583.0 62 46482.0 63 35088.5 64 24236.0 65 18284.5 66 12962.0 67 10098.0 68 7577.5 69 4833.0 70 3559.5 71 2493.5 72 1834.0 73 1480.0 74 1156.5 75 842.0 76 524.5 77 292.0 78 187.0 79 108.0 80 69.0 81 40.0 82 26.5 83 11.5 84 5.5 85 4.5 86 2.5 87 3.0 88 3.5 89 1.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1694396463237174 2 0.0021503345954229456 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 6.719795610696705E-5 10-11 4.367867146952858E-4 12-13 0.006467803275295579 14-15 0.0 16-17 5.207841598289946E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 1.6799489026741762E-5 94-95 5.879821159359617E-4 96-97 0.006081415027680517 98-99 0.0020999361283427203 100-101 0.0034438952504820615 102-103 0.0039646794103110556 104-105 0.011474051005264625 106-107 0.01705148136214289 108-109 0.02474564733639062 110-111 0.015976314064431417 112-113 0.023401688214251273 114-115 0.02640879675003805 116-117 0.025182434051085903 118-119 0.014985144211853652 120-121 0.021839335734764292 122-123 0.014279565672730497 124-125 0.014531558008131624 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2976281.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.94440317144607 #Duplication Level Percentage of deduplicated Percentage of total 1 80.06430023210646 49.59535293217306 2 11.375398098589601 14.092844921094706 3 3.760685212293144 6.9886020297354525 4 1.6906427618478037 4.1890342743514974 5 0.8960411819650962 2.7752368116932487 6 0.5587622331093955 2.076731582682356 7 0.3494866317151155 1.5154118572594264 8 0.24073725206117286 1.1929860320050663 9 0.17410239481913017 0.9706202044011434 >10 0.8003051999436775 8.822233602868968 >50 0.06021702887426772 2.544708713510195 >100 0.02730422733965857 2.9451835749134 >500 0.0013450302236985665 0.5363121370040305 >1k 4.0350906710957E-4 0.5911347851551337 >5k 2.690060447397133E-4 1.163606541152445 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7145 0.24006469819213977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6821 0.22917862930281113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3881 0.13039763382556957 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.3598978053483525E-5 0.0 6 0.0 0.0 0.0 3.3598978053483525E-5 0.0 7 0.0 0.0 0.0 3.3598978053483525E-5 0.0 8 0.0 0.0 0.0 3.3598978053483525E-5 0.0 9 0.0 0.0 0.0 3.3598978053483525E-5 0.0 10-11 0.0 0.0 0.0 6.719795610696705E-5 0.0 12-13 0.0 0.0 0.0 6.719795610696705E-5 0.0 14-15 0.0 0.0 0.0 1.1759642318719234E-4 0.0 16-17 0.0 0.0 0.0 2.0159386832090115E-4 0.0 18-19 0.0 0.0 0.0 2.8559131345461E-4 0.0 20-21 0.0 0.0 0.0 3.52789269561577E-4 0.0 22-23 0.0 0.0 0.0 4.367867146952858E-4 0.0 24-25 0.0 0.0 0.0 5.543831378824782E-4 0.0 26-27 0.0 0.0 0.0 8.063754732836046E-4 0.0 28-29 0.0 0.0 0.0 0.0023687279527705883 0.0 30-31 0.0 0.0 0.0 0.007274178748579183 0.0 32-33 0.0 0.0 0.0 0.018462638440389197 0.0 34-35 0.0 0.0 0.0 0.036673284545377265 0.0 36-37 0.0 0.0 0.0 0.06701316172767288 0.0 38-39 0.0 0.0 0.0 0.11242218056695588 0.0 40-41 0.0 0.0 0.0 0.16285424662523465 0.0 42-43 0.0 0.0 0.0 0.21456307384954582 0.0 44-45 0.0 0.0 0.0 0.2816602330223524 0.0 46-47 0.0 0.0 0.0 0.3619449910811513 0.0 48-49 0.0 0.0 0.0 0.44799197387612255 0.0 50-51 0.0 0.0 0.0 0.5476801417608081 0.0 52-53 0.0 0.0 0.0 0.6426140542509259 0.0 54-55 0.0 0.0 0.0 0.748383637163292 0.0 56-57 0.0 0.0 0.0 0.8746989951553634 0.0 58-59 0.0 0.0 0.0 1.016856271299652 0.0 60-61 0.0 0.0 0.0 1.1813568678495074 0.0 62-63 0.0 0.0 0.0 1.3429007543306564 0.0 64-65 0.0 0.0 0.0 1.5053854121973025 0.0 66-67 0.0 0.0 0.0 1.7054673265057971 0.0 68-69 0.0 0.0 0.0 1.9354523312818919 0.0 70-71 0.0 0.0 0.0 2.192602109814228 0.0 72-73 0.0 0.0 0.0 2.4598147822735825 0.0 74-75 0.0 0.0 0.0 2.71803972810363 0.0 76-77 0.0 0.0 0.0 3.0118291922032903 0.0 78-79 0.0 0.0 0.0 3.355093151486704 0.0 80-81 0.0 0.0 0.0 3.7383230951647377 0.0 82-83 0.0 0.0 0.0 4.138604520204913 0.0 84-85 0.0 0.0 0.0 4.535106060214073 0.0 86-87 0.0 0.0 0.0 4.954186113475172 0.0 88-89 0.0 0.0 0.0 5.410779425732986 0.0 90-91 0.0 0.0 0.0 5.931026001913126 0.0 92-93 0.0 0.0 0.0 6.463217014791278 0.0 94-95 0.0 0.0 0.0 6.994349659860745 0.0 96-97 0.0 0.0 0.0 7.535713193747499 0.0 98-99 0.0 0.0 0.0 8.111599677584206 0.0 100-101 0.0 0.0 0.0 8.76387343802551 0.0 102-103 0.0 0.0 0.0 9.447713438348059 0.0 104-105 0.0 0.0 0.0 10.124044067075655 0.0 106-107 0.0 0.0 0.0 10.783188818528895 0.0 108-109 0.0 0.0 0.0 11.492883232463601 0.0 110-111 0.0 0.0 0.0 12.255227245008115 0.0 112-113 0.0 0.0 0.0 13.055168513994479 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4650 0.0 73.443146 1 GTATCAA 8455 0.0 54.771797 1 ATCAACG 11030 0.0 41.69457 3 TCAACGC 11240 0.0 40.91558 4 TATCAAC 11390 0.0 40.482574 2 CAACGCA 11435 0.0 40.21785 5 AACGCAG 11765 0.0 39.14034 6 GTGGTAT 2120 0.0 37.671986 1 TGGTATC 1975 0.0 37.6559 2 GTACATG 9895 0.0 33.670193 1 ACGCAGA 13770 0.0 33.22969 7 TACATGG 9885 0.0 33.04348 2 CGCAGAG 13980 0.0 32.60284 8 ACATGGG 9880 0.0 30.9515 3 TAAGGTG 1845 0.0 29.989008 5 GCAGAGT 16055 0.0 28.277964 9 GTAAGGT 1835 0.0 26.910238 4 GAGTACT 11390 0.0 26.094301 12-13 AGAGTAC 14480 0.0 25.744616 10-11 CAGAGTA 15110 0.0 25.53756 10-11 >>END_MODULE