FastQCFastQC Report
Thu 26 May 2016
SRR1364128_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364128_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856029
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77090.41534911361837556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22100.11907141537120379No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.063.0480041
GTATCAA37150.047.5780371
TCAACGC40750.041.3391724
CAACGCA40950.040.9941335
ATCAACG41800.040.1539963
GTACATG90950.039.3915181
AACGCAG43250.038.5388226
TACATGG91150.038.066622
ACATGGG93100.036.440233
TATCAAC50750.033.891772
CGCAGAG52950.030.8059528
ACGCAGA53500.030.600537
CATGGGG33150.029.089384
CATGGGA63200.029.0092584
GCAGAGT63700.025.5136879
GAGTACA62600.025.0979651
CATTCCG3302.910383E-1123.4519759
AGTACAT64550.023.2346842
ATGGGAT30450.022.2866125
CAGAGTA60750.022.04886610-11