##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364128_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1856029 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.884457624315136 31.0 31.0 34.0 27.0 34.0 2 30.928234957535686 31.0 31.0 34.0 27.0 34.0 3 31.02176797884085 33.0 31.0 34.0 27.0 34.0 4 34.45115351107122 37.0 35.0 37.0 30.0 37.0 5 34.53667857560415 37.0 35.0 37.0 31.0 37.0 6 34.548492507390776 37.0 35.0 37.0 31.0 37.0 7 34.4463001386293 37.0 35.0 37.0 30.0 37.0 8 34.38468526084452 37.0 35.0 37.0 30.0 37.0 9 35.833626521999385 39.0 37.0 39.0 30.0 39.0 10-11 35.75314690664855 39.0 37.0 39.0 30.0 39.0 12-13 35.64364242153544 39.0 37.0 39.0 30.0 39.0 14-15 36.87239800671218 40.0 37.0 41.0 30.0 41.0 16-17 36.899610135401986 40.0 37.0 41.0 30.0 41.0 18-19 36.919534931835656 40.0 37.0 41.0 30.0 41.0 20-21 36.952394870985316 40.0 37.0 41.0 30.0 41.0 22-23 36.92000825418137 40.0 37.0 41.0 30.0 41.0 24-25 36.94779068646018 40.0 37.0 41.0 30.0 41.0 26-27 36.93055927466651 40.0 37.0 41.0 30.0 41.0 28-29 36.895008914192616 40.0 37.0 41.0 30.0 41.0 30-31 36.84989404799171 40.0 37.0 41.0 30.0 41.0 32-33 36.7519273675142 40.0 37.0 41.0 30.0 41.0 34-35 36.72895870700296 40.0 37.0 41.0 30.0 41.0 36-37 36.666143955724834 40.0 36.0 41.0 30.0 41.0 38-39 36.57855319070984 40.0 36.0 41.0 30.0 41.0 40-41 36.50133861054972 40.0 36.0 41.0 30.0 41.0 42-43 36.39123230294354 40.0 36.0 41.0 30.0 41.0 44-45 36.300007704620995 40.0 36.0 41.0 29.0 41.0 46-47 36.19211041422305 40.0 36.0 41.0 29.0 41.0 48-49 36.08926692416983 40.0 35.0 41.0 29.0 41.0 50-51 35.9769357052072 40.0 35.0 41.0 28.0 41.0 52-53 35.84909395273458 39.5 35.0 41.0 28.0 41.0 54-55 35.68561859755424 39.0 35.0 41.0 28.0 41.0 56-57 35.520441221554194 39.0 35.0 41.0 28.0 41.0 58-59 35.33418362536361 39.0 35.0 41.0 27.5 41.0 60-61 35.11041395366128 38.5 34.0 41.0 27.0 41.0 62-63 34.864177499381746 38.0 34.0 40.0 26.0 41.0 64-65 34.60033005949799 38.0 34.0 40.0 26.0 41.0 66-67 34.305760578094414 37.0 34.0 40.0 26.0 41.0 68-69 33.987870070995655 37.0 34.0 39.5 26.0 41.0 70-71 33.64575930656256 36.0 33.0 39.0 25.5 41.0 72-73 33.29364357992251 36.0 33.0 39.0 25.0 40.5 74-75 33.010614327685616 35.0 33.0 38.5 25.0 40.0 76-77 32.76839774594039 35.0 33.0 37.0 25.0 39.5 78-79 32.44498469582102 35.0 33.0 37.0 24.5 39.0 80-81 32.209644084224976 35.0 33.0 36.5 24.5 39.0 82-83 32.0780289532114 35.0 33.0 36.0 25.0 38.0 84-85 31.841201295884925 35.0 33.0 36.0 24.5 37.0 86-87 31.631985006699786 35.0 33.0 35.5 24.0 37.0 88-89 31.432186943199703 35.0 33.0 35.0 23.5 36.0 90-91 31.276763455743417 35.0 33.0 35.0 23.0 36.0 92-93 31.145477252779994 35.0 33.0 35.0 23.0 36.0 94-95 31.030764874902278 35.0 32.5 35.0 20.5 36.0 96-97 30.938803219130733 35.0 32.0 35.0 20.0 35.5 98-99 30.85764177176111 35.0 32.0 35.0 20.0 35.0 100-101 30.792005135695618 35.0 32.0 35.0 19.5 35.0 102-103 29.717417400266914 34.0 30.0 35.0 17.5 35.0 104-105 30.52129815859558 35.0 32.0 35.0 18.0 35.0 106-107 30.622519906747147 35.0 32.0 35.0 18.0 35.0 108-109 30.610091221635006 35.0 32.0 35.0 18.0 35.0 110-111 30.539458973970774 35.0 32.0 35.0 16.0 35.0 112-113 30.46829090493737 35.0 32.0 35.0 11.5 35.0 114-115 30.370340388000404 35.0 32.0 35.0 6.0 35.0 116-117 30.27948916746452 35.0 32.0 35.0 2.0 35.0 118-119 30.159896208518294 35.0 32.0 35.0 2.0 35.0 120-121 30.029129932775835 35.0 32.0 35.0 2.0 35.0 122-123 29.874986867123305 35.0 32.0 35.0 2.0 35.0 124-125 29.0712575072911 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36752714316267543 1101 2 0.32165319821568517 1101 3 0.3567429514119773 1101 4 0.4237457877562818 1101 5 0.4555592425583157 1101 6 0.3652173111600021 1101 7 0.3317082270142322 1101 8 0.38820758850656745 1101 9 0.29723265392409104 1101 10-11 0.33917439703377283 1101 12-13 0.4254518146290067 1101 14-15 0.4570070116137046 1101 16-17 0.45657127168017553 1101 18-19 0.5013200520282552 1101 20-21 0.49170208006783866 1101 22-23 0.5516135105991751 1101 24-25 0.5322871012766797 1101 26-27 0.48723255744511107 1101 28-29 0.5521290669533343 1101 30-31 0.6483544224182296 1101 32-33 0.49897391894918997 1101 34-35 0.5978150083104055 1101 36-37 0.5214988250648744 1101 38-39 0.3892083854907753 1101 40-41 0.4913740640745914 1101 42-43 0.43539595788580243 1101 44-45 0.43273846945901084 1101 46-47 0.525091715888756 1101 48-49 0.3335704773012367 1101 50-51 0.4729132966568841 1101 52-53 0.40374752406911796 1101 54-55 0.42604309398132756 1101 56-57 0.39699944639630047 1101 58-59 0.30934899046497577 1101 60-61 0.3449902723832565 1101 62-63 0.39817715511729546 1101 64-65 0.5241587137854609 1101 66-67 0.46031471591079054 1101 68-69 0.5254797170110166 1101 70-71 0.4892159996350216 1101 72-73 0.5772256455940763 1101 74-75 0.5184754799037492 1101 76-77 0.5482208040832859 1101 78-79 0.4720518361011088 1101 80-81 0.392269540096855 1101 82-83 0.3802626172569177 1101 84-85 0.40410489386677284 1101 86-87 0.3657343404599125 1101 88-89 0.439330607810156 1101 90-91 0.48964778519797747 1101 92-93 0.4531872060908171 1101 94-95 0.4658473121407347 1101 96-97 0.48710967491170365 1101 98-99 0.38703738899020124 1101 100-101 0.43320463359013317 1101 102-103 0.4739507650124857 1101 104-105 0.4459923707381179 1101 106-107 0.45720614253043124 1101 108-109 0.4437203308108266 1101 110-111 0.4654045938680085 1101 112-113 0.4670401238316728 1101 114-115 0.47841524355076714 1101 116-117 0.4605490549450195 1101 118-119 0.4961897652100191 1101 120-121 0.4569477959961361 1101 122-123 0.5738808487151168 1101 124-125 0.6462776447732708 1102 1 0.48462705382364035 1102 2 0.4302412870704515 1102 3 0.43596443458889667 1102 4 0.46116049062573694 1102 5 0.4677017123283065 1102 6 0.4575160215146852 1102 7 0.4801016204759492 1102 8 0.5507063181934484 1102 9 0.6720219749929228 1102 10-11 0.541960012718171 1102 12-13 0.6100513036908026 1102 14-15 0.7009442914462127 1102 16-17 0.6849759831663818 1102 18-19 0.5889779310927921 1102 20-21 0.6342189395532145 1102 22-23 0.6587318971629372 1102 24-25 0.6391515631072338 1102 26-27 0.6841028920176626 1102 28-29 0.7039004246570997 1102 30-31 0.6458961166112189 1102 32-33 0.602354999508897 1102 34-35 0.7068979849563704 1102 36-37 0.7631434051748514 1102 38-39 0.733850297839794 1102 40-41 0.7995696761614397 1102 42-43 0.725032864132487 1102 44-45 0.7844656609439085 1102 46-47 0.7949556555292645 1102 48-49 0.8049935685808052 1102 50-51 0.8031819521960912 1102 52-53 0.8287185912658899 1102 54-55 0.8393274873383874 1102 56-57 0.8622118268920715 1102 58-59 0.8208095244391131 1102 60-61 0.8251048563500305 1102 62-63 0.8162364090019025 1102 64-65 0.7244322464996955 1102 66-67 0.8770791122297297 1102 68-69 0.8536436860634993 1102 70-71 0.8645190994472287 1102 72-73 0.8375934345074043 1102 74-75 0.736966026636857 1102 76-77 0.7300104363702218 1102 78-79 0.8113247881598795 1102 80-81 0.7142056999854631 1102 82-83 0.7201667615319494 1102 84-85 0.7539547107968971 1102 86-87 0.7360555376664202 1102 88-89 0.7619508407921316 1102 90-91 0.8295130444473422 1102 92-93 0.7578966101584932 1102 94-95 0.8040316903160871 1102 96-97 0.8265538176065093 1102 98-99 0.7862409216258328 1102 100-101 0.7347221561892994 1102 102-103 0.8010573233581582 1102 104-105 0.748128951219293 1102 106-107 0.7880900639722022 1102 108-109 0.8199571538545882 1102 110-111 0.8551238343303886 1102 112-113 0.8822804201907246 1102 114-115 0.855638739648267 1102 116-117 0.8958801547335113 1102 118-119 0.9067398656009473 1102 120-121 0.8112805599809931 1102 122-123 0.8167451362525071 1102 124-125 0.9577909631013952 1103 1 0.2940271628816902 1103 2 0.37520225939628205 1103 3 0.38752044152053244 1103 4 0.3626826719190248 1103 5 0.36278392765124323 1103 6 0.3805206534492811 1103 7 0.3358831029927529 1103 8 0.4225636627678071 1103 9 0.4398752682096543 1103 10-11 0.4475505058116198 1103 12-13 0.5437076346538632 1103 14-15 0.5885804554920071 1103 16-17 0.5419359873125771 1103 18-19 0.44712741505194487 1103 20-21 0.5554264293820879 1103 22-23 0.529632158119334 1103 24-25 0.6163007868267556 1103 26-27 0.6692582749255394 1103 28-29 0.6559058549539571 1103 30-31 0.51943033894959 1103 32-33 0.37796868535665595 1103 34-35 0.5527861574511306 1103 36-37 0.5590484767049091 1103 38-39 0.6842916025004513 1103 40-41 0.6573493386548961 1103 42-43 0.6560160874980383 1103 44-45 0.5546594567649663 1103 46-47 0.6548410122532928 1103 48-49 0.5617887451054244 1103 50-51 0.5433346114095343 1103 52-53 0.5303160187530409 1103 54-55 0.5668237891237311 1103 56-57 0.5769727896243211 1103 58-59 0.5875993224942562 1103 60-61 0.49439879921750673 1103 62-63 0.43234284370242904 1103 64-65 0.44231891048677596 1103 66-67 0.4512613284899558 1103 68-69 0.3527715800577482 1103 70-71 0.3537175696572348 1103 72-73 0.39599938131376433 1103 74-75 0.2844698902580589 1103 76-77 0.319511366230671 1103 78-79 0.36234719357903344 1103 80-81 0.3441546637961679 1103 82-83 0.2416683717646606 1103 84-85 0.23336428386606656 1103 86-87 0.33554578226410214 1103 88-89 0.29257283004842094 1103 90-91 0.2678548603796287 1103 92-93 0.2903263551071156 1103 94-95 0.30414113431301715 1103 96-97 0.33325381644866425 1103 98-99 0.37277783512868723 1103 100-101 0.35691927337377294 1103 102-103 0.39171658146569754 1103 104-105 0.5061670200868633 1103 106-107 0.5303782595136148 1103 108-109 0.4928721216582055 1103 110-111 0.46930569496655394 1103 112-113 0.5002162512731516 1103 114-115 0.5861394445558226 1103 116-117 0.5159644789898827 1103 118-119 0.5996960416883468 1103 120-121 0.47240534929440514 1103 122-123 0.5101785791401063 1103 124-125 0.6173100683843167 1104 1 0.3852768198945 1104 2 0.3332539845545952 1104 3 0.3523350670323211 1104 4 0.38846562424514985 1104 5 0.4783829850417405 1104 6 0.40148187841121796 1104 7 0.3529598298996248 1104 8 0.4136293172411669 1104 9 0.41029820504082437 1104 10-11 0.4387008982965739 1104 12-13 0.4327370212582622 1104 14-15 0.523415579532319 1104 16-17 0.524746007380692 1104 18-19 0.549004429261025 1104 20-21 0.4776751341010552 1104 22-23 0.5222555124533415 1104 24-25 0.601733919120278 1104 26-27 0.5698900438786083 1104 28-29 0.6639378079095692 1104 30-31 0.6193634900737806 1104 32-33 -0.17004777264826032 1104 34-35 0.44597496988152585 1104 36-37 0.5673992059898296 1104 38-39 0.6142234892797092 1104 40-41 0.5673864888752149 1104 42-43 0.6868635483167935 1104 44-45 0.6439391567655122 1104 46-47 0.6561381763544958 1104 48-49 0.682412094059238 1104 50-51 0.7057437534304469 1104 52-53 0.5074500715972761 1104 54-55 0.6617537659351029 1104 56-57 0.6566440061393592 1104 58-59 0.6913629007518338 1104 60-61 0.7874401944086884 1104 62-63 0.8109791804378332 1104 64-65 0.774790920041994 1104 66-67 0.7155355137945065 1104 68-69 0.7295477156816119 1104 70-71 0.7709967262584598 1104 72-73 0.7280261800461929 1104 74-75 0.7238266614977107 1104 76-77 0.7561690300440489 1104 78-79 0.8325530396948082 1104 80-81 0.7701725959734702 1104 82-83 0.7133438171099868 1104 84-85 0.7449719119011675 1104 86-87 0.7416297024866232 1104 88-89 0.7044514852158343 1104 90-91 0.668015190077746 1104 92-93 0.6761794113247355 1104 94-95 0.6778739647778096 1104 96-97 0.654953069140106 1104 98-99 0.6856944636856674 1104 100-101 0.6757165854302407 1104 102-103 0.7227450857187385 1104 104-105 0.7760508617585913 1104 106-107 0.7719262481291267 1104 108-109 0.6633363514569339 1104 110-111 0.732710844885581 1104 112-113 0.687036934880247 1104 114-115 0.7475353620667313 1104 116-117 0.7901320289046083 1104 118-119 0.8990935947773835 1104 120-121 0.9486309568524973 1104 122-123 1.0302023057833516 1104 124-125 1.2211293316209293 1105 1 0.3958723326022877 1105 2 0.35607266195159326 1105 3 0.3682541964686692 1105 4 0.3011165195313197 1105 5 0.3248375276751503 1105 6 0.4078693909535005 1105 7 0.3365022874627499 1105 8 0.3015584701587386 1105 9 0.2861125482390321 1105 10-11 0.4314311590725879 1105 12-13 0.4716372312445003 1105 14-15 0.4414627591998581 1105 16-17 0.5507545619926262 1105 18-19 0.5339557600359015 1105 20-21 0.5002768429546194 1105 22-23 0.4503325091328705 1105 24-25 0.45840020650145163 1105 26-27 0.5225892204842779 1105 28-29 0.5231254818205855 1105 30-31 0.5478837335750697 1105 32-33 0.24940116862799755 1105 34-35 0.5408807346762501 1105 36-37 0.4890403312264553 1105 38-39 0.5567820371679488 1105 40-41 0.6042187740013674 1105 42-43 0.6512779918532487 1105 44-45 0.6409105189866082 1105 46-47 0.5786143330890567 1105 48-49 0.6359069943933591 1105 50-51 0.6072894280241741 1105 52-53 0.7033078860852982 1105 54-55 0.6464002028926856 1105 56-57 0.7407606933219242 1105 58-59 0.7858189536311215 1105 60-61 0.6825121121106292 1105 62-63 0.8033982116047582 1105 64-65 0.7436338234379818 1105 66-67 0.6955859156647293 1105 68-69 0.7984733722765185 1105 70-71 0.728514466475481 1105 72-73 0.6655741510411914 1105 74-75 0.7649871975315605 1105 76-77 0.7400838770023483 1105 78-79 0.5650850031178933 1105 80-81 0.6027653312770198 1105 82-83 0.533350695969645 1105 84-85 0.4874896003974385 1105 86-87 0.4490639136934824 1105 88-89 0.410366270109229 1105 90-91 0.38422124037100147 1105 92-93 0.43835227758522066 1105 94-95 0.5286651629406869 1105 96-97 0.55034229363919 1105 98-99 0.49029024016927636 1105 100-101 0.41664308507336756 1105 102-103 0.4570797758597287 1105 104-105 0.5355176984141252 1105 106-107 0.5017770079069344 1105 108-109 0.5275854178974058 1105 110-111 0.5611390254530626 1105 112-113 0.5588650306257001 1105 114-115 0.5634300534490215 1105 116-117 0.5947234091245264 1105 118-119 0.6746934261677389 1105 120-121 0.6823059488357828 1105 122-123 0.687963521427843 1105 124-125 0.7846593503438442 1106 1 -0.049174850507132106 1106 2 0.10672517795694247 1106 3 0.011469157691895049 1106 4 0.03982409702080503 1106 5 -0.03853802527193295 1106 6 -0.06901985560323709 1106 7 0.0326030814511995 1106 8 -0.1449907043290679 1106 9 0.01941905648390474 1106 10-11 0.03335160901646361 1106 12-13 0.08901014952366637 1106 14-15 0.09208029153092667 1106 16-17 0.12162187519518142 1106 18-19 0.17046487629446716 1106 20-21 0.12177334971299558 1106 22-23 0.19212499315315057 1106 24-25 0.14255042357963532 1106 26-27 0.16357848959459176 1106 28-29 0.2652355740339587 1106 30-31 0.30144534072250195 1106 32-33 0.22056917484048455 1106 34-35 0.21461901578608433 1106 36-37 0.2670178519731863 1106 38-39 0.3129563993886535 1106 40-41 0.20371586766535188 1106 42-43 0.13295442746535002 1106 44-45 0.18763083155868543 1106 46-47 0.20064680767784182 1106 48-49 0.20127604012073874 1106 50-51 0.17012638616165532 1106 52-53 0.23111856161619926 1106 54-55 0.25888860574058725 1106 56-57 0.38713424463900026 1106 58-59 0.37867843971710613 1106 60-61 0.3218770110552285 1106 62-63 0.39155079168277496 1106 64-65 0.3931391824755579 1106 66-67 0.38070087483762904 1106 68-69 0.3441031040427234 1106 70-71 0.3582993489149544 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0.8539930345208369 2203 104-105 0.8194872931506545 2203 106-107 0.7694232573502546 2203 108-109 0.8238718332105357 2203 110-111 0.710886628575512 2203 112-113 0.7306078189862433 2203 114-115 0.6904493335034694 2203 116-117 0.7639122939987786 2203 118-119 0.800254308117843 2203 120-121 0.8060912297809182 2203 122-123 0.776241508806951 2203 124-125 0.7532410332417747 2204 1 0.5712712871222827 2204 2 0.5919509706766419 2204 3 0.5587813602794753 2204 4 0.637991605775305 2204 5 0.6084412274405722 2204 6 0.5477449561052481 2204 7 0.5845257782945126 2204 8 0.5885794609296155 2204 9 0.7941312094077304 2204 10-11 0.8697296768936411 2204 12-13 0.7723730446165789 2204 14-15 0.8754490778602175 2204 16-17 0.8090020233756192 2204 18-19 0.7607469968355005 2204 20-21 0.8261913335672091 2204 22-23 0.8732456076661137 2204 24-25 0.8341550950133438 2204 26-27 0.8574664902229898 2204 28-29 0.8331852844131831 2204 30-31 0.8345988118643106 2204 32-33 0.8751408376134435 2204 34-35 0.8260733530029967 2204 36-37 0.7751958382598971 2204 38-39 0.7709268621231047 2204 40-41 0.7443297344955369 2204 42-43 0.7999017408081812 2204 44-45 0.7552477187560811 2204 46-47 0.8212470676524006 2204 48-49 0.6817766178419831 2204 50-51 0.701321242922269 2204 52-53 0.7917034030215859 2204 54-55 0.8639359383407879 2204 56-57 0.904199129322798 2204 58-59 0.8145432732168274 2204 60-61 0.7994026411007837 2204 62-63 0.824097782211183 2204 64-65 0.8450572550150426 2204 66-67 0.7548614053276239 2204 68-69 0.7273712959272629 2204 70-71 0.7356509024276505 2204 72-73 0.735160362556087 2204 74-75 0.8727561570029749 2204 76-77 0.8328473980296422 2204 78-79 0.9205066042932444 2204 80-81 0.8649018889854077 2204 82-83 0.7890230134773759 2204 84-85 0.756527198715574 2204 86-87 0.7335889021266446 2204 88-89 0.8206689763439599 2204 90-91 0.731608960851208 2204 92-93 0.7388770468571977 2204 94-95 0.7281586571004475 2204 96-97 0.7242461833442952 2204 98-99 0.7241681958013118 2204 100-101 0.7578374729237218 2204 102-103 0.8443355531919714 2204 104-105 0.6889934324011513 2204 106-107 0.7166072188698642 2204 108-109 0.7130901379798757 2204 110-111 0.6689627752196934 2204 112-113 0.7313129189671344 2204 114-115 0.7509285314963776 2204 116-117 0.7989466247872414 2204 118-119 0.7415259180303657 2204 120-121 0.6615576757447172 2204 122-123 0.6207145218580514 2204 124-125 0.7875292457146728 2205 1 0.38030323283377854 2205 2 0.4883644803835949 2205 3 0.359359736105727 2205 4 0.5049191517492844 2205 5 0.42825245005224133 2205 6 0.4446846257648289 2205 7 0.45303580534272925 2205 8 0.4173544085136811 2205 9 0.5002918822522773 2205 10-11 0.5889413172322975 2205 12-13 0.5595953602005181 2205 14-15 0.6613945052701453 2205 16-17 0.542275938645858 2205 18-19 0.49461009121619526 2205 20-21 0.5557511197422116 2205 22-23 0.5642566750305917 2205 24-25 0.553797366263808 2205 26-27 0.5574998003492624 2205 28-29 0.5187596990352219 2205 30-31 0.5417852965556591 2205 32-33 0.7038012503789304 2205 34-35 0.6476886240453865 2205 36-37 0.6202652393578347 2205 38-39 0.5548156643189657 2205 40-41 0.5906565178376724 2205 42-43 0.62431536254919 2205 44-45 0.639183935365935 2205 46-47 0.5908825368350321 2205 48-49 0.6595368569546736 2205 50-51 0.7393718102947489 2205 52-53 0.6361861257692993 2205 54-55 0.642834223419456 2205 56-57 0.6652619924331802 2205 58-59 0.6480706655440969 2205 60-61 0.597765843514324 2205 62-63 0.576286447565451 2205 64-65 0.6355475640690003 2205 66-67 0.7048081891499081 2205 68-69 0.6074898519682961 2205 70-71 0.5621361539441168 2205 72-73 0.5979591533884943 2205 74-75 0.6623386900930299 2205 76-77 0.6106983647235182 2205 78-79 0.5973548661648564 2205 80-81 0.5665601931098578 2205 82-83 0.5541481115071747 2205 84-85 0.5434404203349459 2205 86-87 0.5458588321853775 2205 88-89 0.5661726094484223 2205 90-91 0.5752624315062853 2205 92-93 0.6009061036904768 2205 94-95 0.5783516157598818 2205 96-97 0.5988965770639894 2205 98-99 0.605214033736047 2205 100-101 0.597845835465936 2205 102-103 0.6500574926615705 2205 104-105 0.5553868159897632 2205 106-107 0.5983432666283832 2205 108-109 0.5081933493561337 2205 110-111 0.5314754790816671 2205 112-113 0.5137082671529853 2205 114-115 0.5557922100546584 2205 116-117 0.49374634390185435 2205 118-119 0.4594016522535682 2205 120-121 0.5152892101311686 2205 122-123 0.568740721822838 2205 124-125 0.4972319953505604 2206 1 0.5725236806978984 2206 2 0.5034361906631766 2206 3 0.5573879302527658 2206 4 0.6306283909125909 2206 5 0.5511821975691475 2206 6 0.621400863561071 2206 7 0.6337919843432047 2206 8 0.7101892782798274 2206 9 0.8006161058697856 2206 10-11 0.7099437186646895 2206 12-13 0.678462482957876 2206 14-15 0.7593024009620848 2206 16-17 0.7105501132668977 2206 18-19 0.7713373683427065 2206 20-21 0.7005894011150815 2206 22-23 0.6618004996594848 2206 24-25 0.6893660127511794 2206 26-27 0.8021098095436656 2206 28-29 0.7221472611881694 2206 30-31 0.7665426581254806 2206 32-33 0.9550454433887623 2206 34-35 0.8490516788851465 2206 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0.5955534459152965 2206 104-105 0.5261288102069699 2206 106-107 0.5681559911955816 2206 108-109 0.5052425968669922 2206 110-111 0.4545181150922666 2206 112-113 0.5396034358581474 2206 114-115 0.6374726683229675 2206 116-117 0.6537913027085196 2206 118-119 0.5852299800823602 2206 120-121 0.6195390498340458 2206 122-123 0.6542698233120916 2206 124-125 0.636774422672449 2207 1 0.3398667702009526 2207 2 0.3495786573690438 2207 3 0.3307112041540883 2207 4 0.34726861458702274 2207 5 0.3277884706605434 2207 6 0.3250529551462833 2207 7 0.4230857160596315 2207 8 0.42927187287362756 2207 9 0.4097676001762238 2207 10-11 0.45001132396512844 2207 12-13 0.3102558355964433 2207 14-15 0.3925310718226527 2207 16-17 0.39688426392907417 2207 18-19 0.3453463108819008 2207 20-21 0.4071302509151806 2207 22-23 0.41445792792131186 2207 24-25 0.3308913111131986 2207 26-27 0.390372264562842 2207 28-29 0.26709369101438085 2207 30-31 0.372245653395332 2207 32-33 0.5005469911359341 2207 34-35 0.4766487320633104 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0.48813570694727915 2208 100-101 0.36982091116508187 2208 102-103 0.4703232786081166 2208 104-105 0.3693048872362539 2208 106-107 0.35290483952229934 2208 108-109 0.3879675348175837 2208 110-111 0.353292349444299 2208 112-113 0.3650138321934193 2208 114-115 0.38613461747405964 2208 116-117 0.3808545897617037 2208 118-119 0.40394448188093435 2208 120-121 0.46690344667359795 2208 122-123 0.43860098011245796 2208 124-125 0.4546141833356394 2209 1 0.12665806342094044 2209 2 0.09607992504311724 2209 3 0.05858211307880268 2209 4 0.1257467802842882 2209 5 0.14821570328233946 2209 6 0.16014010473182339 2209 7 0.17635111938204773 2209 8 0.25102511181695775 2209 9 0.13712638802002175 2209 10-11 0.2878509138833891 2209 12-13 0.34566050506305146 2209 14-15 0.2817111609206506 2209 16-17 0.22518947750931062 2209 18-19 0.14786458030232552 2209 20-21 0.1497219347808425 2209 22-23 0.3057525911818644 2209 24-25 0.35771410374866264 2209 26-27 0.31306050333779467 2209 28-29 0.15100577649457847 2209 30-31 0.09069656530279957 2209 32-33 0.23415815436520404 2209 34-35 0.20904225465773152 2209 36-37 0.1623543561763654 2209 38-39 0.20715197595045964 2209 40-41 0.2527854108707288 2209 42-43 0.2619269011923109 2209 44-45 0.2943647774728504 2209 46-47 0.27028811652122897 2209 48-49 0.26294026094881673 2209 50-51 0.3524546833098725 2209 52-53 0.3258087041807727 2209 54-55 0.26976451078391506 2209 56-57 0.28685239184775213 2209 58-59 0.16109531027287005 2209 60-61 0.1797255991128921 2209 62-63 0.19272236497570816 2209 64-65 0.2413007548217152 2209 66-67 0.25155212632630963 2209 68-69 0.253128435289824 2209 70-71 0.23900654092615525 2209 72-73 0.16854373784311605 2209 74-75 0.18702718843274369 2209 76-77 0.14264671072543678 2209 78-79 0.29481219820772964 2209 80-81 0.26360589444596627 2209 82-83 0.22704727034449945 2209 84-85 0.22979650991209155 2209 86-87 0.21098815406190496 2209 88-89 0.14103726802608207 2209 90-91 0.166622809518028 2209 92-93 0.18999046388750784 2209 94-95 0.11168189997686895 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-2.6476814874091374 2216 116-117 -2.8766933111831463 2216 118-119 -3.0022671881938763 2216 120-121 -3.105981245378363 2216 122-123 -3.111805186568251 2216 124-125 -3.3783479964021694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37020.0 3 6737.0 4 3882.0 5 3316.0 6 4040.0 7 4402.0 8 4967.0 9 5427.0 10 6097.0 11 6635.0 12 7381.0 13 7373.0 14 7603.0 15 6956.0 16 6163.0 17 5690.0 18 5478.0 19 5324.0 20 5380.0 21 6051.0 22 7174.0 23 8829.0 24 11983.0 25 14513.0 26 18140.0 27 20355.0 28 24692.0 29 30692.0 30 36486.0 31 43401.0 32 54704.0 33 72471.0 34 99094.0 35 146527.0 36 264706.0 37 550571.0 38 303784.0 39 1985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.06701283740549 18.91046080190206 12.31517200087914 23.7073543598133 2 15.372969192678646 20.54397001653118 40.34863793683942 23.734422853950758 3 18.918747760494416 25.85756149100463 29.407482303169786 25.816208445331167 4 12.041432256625816 16.50557009135361 38.42442894520624 33.02856870681434 5 12.896723259256854 39.15628053252788 34.87245691953351 13.074539288681752 6 31.32198039793407 37.2674312801137 18.147617768380186 13.262970553572048 7 28.12810904519919 32.92483266330052 21.697960439270005 17.249097852230282 8 25.667506392732893 36.069800356075625 20.499805821246134 17.762887429945344 9 26.557274575845195 16.227462459550782 20.359538973708418 36.855723990895605 10-11 24.292381147933988 27.259355413335008 28.60586964047626 19.842393798254744 12-13 24.703699138371263 25.099579683387674 28.31173053140319 21.884990646837874 14-15 21.957813687502338 26.767599343246033 25.859876958845625 25.414710010406004 16-17 21.870616723826146 30.030650263886937 26.48865842736007 21.610074584926853 18-19 21.631836052563507 29.016296420692246 28.081578259521145 21.27028926722311 20-21 22.57231824687503 28.343422151773247 27.784898517718364 21.29936108363336 22-23 22.09253407485565 28.538387459857155 27.500220099932143 21.868858365355052 24-25 21.846825371887483 28.51896737228405 27.925095917661963 21.709111338166505 26-27 21.77385689506507 28.84127928821639 27.620434088527055 21.76442972819148 28-29 21.536861081183268 28.838713032343193 27.895809939510546 21.728615946962986 30-31 21.753011162203386 28.662439704302233 27.8150699902736 21.769479143220785 32-33 21.460097590836266 28.725129978913554 27.713143072283035 22.10162935796714 34-35 21.99110357057055 28.74625241363657 27.608968801284274 21.65367521450861 36-37 22.075452952423856 28.462984284221875 27.47595989204996 21.98560287130431 38-39 21.601173425273295 28.777415893471602 27.67600364259783 21.945407038657272 40-41 21.867599337842435 28.58416326292023 27.85824718762998 21.689990211607352 42-43 21.72356423552516 28.512216965799208 27.72236415075598 22.04185464791965 44-45 21.64721676746871 28.727187589236536 27.68226583318334 21.943329810111422 46-47 21.78661922212938 28.752775470243087 27.425449035423433 22.0351562722041 48-49 21.706411224400572 28.69215332058613 27.536106333690768 22.065329121322534 50-51 21.802166011825044 28.701497239704516 27.498936265811203 21.997400482659238 52-53 21.937500456757206 28.631382183843492 27.53367620123085 21.89744115816846 54-55 21.65661602282464 28.569232526304795 27.742889356673995 22.03126209419657 56-57 21.701421350812282 28.86908619484577 27.45225430779401 21.977238146547933 58-59 21.737578344941294 28.501563515764257 27.58004459145877 22.18081354783568 60-61 21.712057707645304 28.712633344330058 27.62162997467686 21.953678973347778 62-63 21.569827982188382 28.594161555612036 27.77828755241318 22.0577229097864 64-65 21.503100331200038 28.53985606494509 27.610162720019577 22.346880883835293 66-67 21.538768885274763 28.787503911592065 27.32930570188309 22.344421501250086 68-69 21.686679470096124 28.514197566626475 27.570265000668687 22.228857962608718 70-71 21.529699642687657 28.474536995051665 27.707394245806743 22.28836911645394 72-73 21.668232459287136 28.473690622842522 27.558202813011874 22.299874104858468 74-75 21.500931940588618 28.580567177553295 27.523988841077397 22.394512040780693 76-77 21.600697996562534 28.492710207712452 27.655921046041946 22.250670749683067 78-79 21.7799653721144 28.419232961198627 27.50918830232662 22.29161336436035 80-81 21.59712124653577 28.656044783978434 27.47943119062889 22.26740277885691 82-83 21.7819147280127 28.581604041630243 27.465044397885407 22.171436832471656 84-85 21.79208442128887 28.322714345852773 27.51290995450552 22.372291278352833 86-87 21.665508563422325 28.27232741396854 27.69045158988582 22.371712432723314 88-89 21.859235607579226 28.200252027249263 27.683311925003622 22.257200440167892 90-91 21.7955158387746 28.375057245647977 27.626587314905738 22.20283960067168 92-93 21.807405370394378 28.12613256846702 27.70963157200977 22.356830489128832 94-95 21.642002517613417 27.9244521105379 27.764739825412633 22.668805546436054 96-97 21.898025529745325 27.857239313826867 27.65917256866237 22.585562587765434 98-99 21.664807274246133 27.912311702702088 27.625963466455733 22.796917556596046 100-101 21.5914513471021 27.97085814391091 27.765376246119487 22.672314262867506 102-103 21.8158551587366 27.779310523907053 27.695000470643222 22.709833846713128 104-105 21.622238650494104 27.797609821690134 27.72714497823705 22.853006549578712 106-107 21.570218582117267 27.98256298480672 27.616370518179384 22.830847914896633 108-109 21.67718205885265 27.992720654156404 27.34832486853731 22.981772418453634 110-111 21.60390402822763 28.09766564655394 27.509057897485434 22.789372427732996 112-113 21.66254423797575 28.074727036232183 27.615726328099864 22.647002397692198 114-115 21.483157011823913 28.205227414161637 27.46237722882113 22.849238345193324 116-117 21.565807896000592 28.213950908709677 27.41961622488113 22.8006249704086 118-119 21.49726934808375 28.20533224848892 27.333559071916685 22.963839331510645 120-121 21.509525166646775 28.278514265082922 27.420116591168487 22.791843977101816 122-123 21.758846911819294 28.362226914265122 27.301024974486566 22.577901199429018 124-125 21.567758407770228 28.246920515143348 27.299345085216846 22.885975991869575 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8879.0 1 4926.5 2 1186.5 3 1345.0 4 1116.5 5 880.5 6 765.0 7 701.5 8 713.5 9 710.0 10 636.5 11 553.5 12 538.5 13 593.5 14 634.5 15 655.5 16 757.5 17 899.5 18 1077.5 19 1351.5 20 1781.5 21 2467.5 22 3360.0 23 4527.0 24 5996.5 25 7978.0 26 11025.0 27 14420.5 28 17160.0 29 20165.5 30 24409.5 31 28900.0 32 34380.5 33 40010.5 34 45423.0 35 49963.0 36 55023.0 37 60528.5 38 62243.0 39 63091.5 40 64101.5 41 65273.0 42 66177.5 43 67492.0 44 69122.5 45 70726.5 46 70583.0 47 68660.0 48 67851.5 49 66622.5 50 63917.0 51 60378.5 52 57348.0 53 54840.0 54 52056.0 55 47889.5 56 42775.0 57 38029.5 58 34736.0 59 30976.5 60 26083.0 61 21443.5 62 17426.5 63 12938.0 64 9380.0 65 7626.0 66 5763.5 67 4450.5 68 3409.0 69 2293.5 70 1701.5 71 1393.5 72 1249.5 73 1162.0 74 899.5 75 712.5 76 495.0 77 266.5 78 153.0 79 81.5 80 53.5 81 39.0 82 24.5 83 17.5 84 12.0 85 11.0 86 11.0 87 8.5 88 5.5 89 4.0 90 4.0 91 2.5 92 1.5 93 2.0 94 3.0 95 3.5 96 2.0 97 0.5 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47256804715874584 2 0.46394749219974474 3 0.4592061869722941 4 0.45705104823254383 5 0.464109127605226 6 0.5222439951099902 7 0.5173410544770582 8 0.5275779634908722 9 0.5326964179977791 10-11 0.5509612188171629 12-13 0.5601474976953486 14-15 0.5377071155676986 16-17 0.5322384510155822 18-19 0.5426369954348774 20-21 0.5436876255705057 22-23 0.5536820814760977 24-25 0.5482134169239813 26-27 0.554921286251454 28-29 0.5345821643950606 30-31 0.5399969504786832 32-33 0.541828818407471 34-35 0.5391887734512769 36-37 0.4883274991931699 38-39 0.4550844841325216 40-41 0.454330185573609 42-43 0.466560597921692 44-45 0.47205620170805523 46-47 0.4751811528806931 48-49 0.48967446090551386 50-51 0.4721370194107959 52-53 0.4723794725190178 54-55 0.46076866255861304 56-57 0.44420103349678264 58-59 0.4628160443613758 60-61 0.4584788276476284 62-63 0.47453461125876806 64-65 0.47450767202452115 66-67 0.4824816853615972 68-69 0.49180266041101733 70-71 0.4873576867602823 72-73 0.4985913474412307 74-75 0.4890279192835888 76-77 0.4864417527958884 78-79 0.49845665126999633 80-81 0.4912908149603266 82-83 0.4930149259521268 84-85 0.49037488099593274 86-87 0.4794914303601937 88-89 0.4852564264890257 90-91 0.4701973945450206 92-93 0.47060148305872374 94-95 0.4657524208942856 96-97 0.45362976548319023 98-99 0.4178275231690884 100-101 0.414379301185488 102-103 0.40381912136071146 104-105 0.40891063663337157 106-107 0.4066477409566337 108-109 0.4194977556923949 110-111 0.44148017083784785 112-113 0.44236916556799494 114-115 0.4352841469610658 116-117 0.4278758575431742 118-119 0.4719484447710677 120-121 0.4651597577408542 122-123 0.4301118139856651 124-125 0.4393789105665914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1856029.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.54811619478031 #Duplication Level Percentage of deduplicated Percentage of total 1 84.33656062054278 52.75092993161843 2 9.289148913813996 11.620375312237103 3 2.7535870991721683 5.166950574944064 4 1.1926036073272828 2.9838043602168414 5 0.5711555542430946 1.7862351986045615 6 0.37477370233125623 1.4064833448098055 7 0.24056321951531262 1.0532743344503952 8 0.16430928347393106 0.8221788923686829 9 0.11375593844427406 0.6403697690092859 >10 0.8074090587684503 10.165450209031993 >50 0.10111003683629441 4.382836355970187 >100 0.054029771209495245 5.786704719324656 >500 5.959165930622941E-4 0.2020221548544983 >1k 1.98638864354098E-4 0.27458115758206714 >5k 1.98638864354098E-4 0.9578036849774461 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7709 0.41534911361837556 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2210 0.11907141537120379 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.3878468493757374E-5 0.0 4 0.0 0.0 0.0 1.0775693698751475E-4 0.0 5 0.0 0.0 0.0 1.0775693698751475E-4 0.0 6 0.0 0.0 0.0 1.0775693698751475E-4 0.0 7 0.0 0.0 0.0 1.0775693698751475E-4 0.0 8 0.0 0.0 0.0 1.0775693698751475E-4 0.0 9 0.0 0.0 0.0 1.0775693698751475E-4 0.0 10-11 0.0 0.0 0.0 1.0775693698751475E-4 0.0 12-13 0.0 0.0 0.0 1.0775693698751475E-4 0.0 14-15 0.0 0.0 0.0 1.0775693698751475E-4 0.0 16-17 0.0 0.0 0.0 1.0775693698751475E-4 0.0 18-19 0.0 0.0 0.0 1.616354054812721E-4 0.0 20-21 0.0 0.0 0.0 1.616354054812721E-4 0.0 22-23 0.0 0.0 0.0 2.6939234246878686E-4 0.0 24-25 0.0 0.0 0.0 7.004200904188458E-4 0.0 26-27 0.0 0.0 0.0 0.0012661440096032983 0.0 28-29 0.0 0.0 0.0 0.002747801893181626 0.0 30-31 0.0 0.0 0.0 0.008782190364482452 0.0 32-33 0.0 0.0 0.0 0.024460824696165846 0.0 34-35 0.0 0.0 0.0 0.04911022403205984 0.0 36-37 0.0 0.0 0.0 0.09003092085306857 0.0 38-39 0.0 0.0 0.0 0.14892008691674538 0.0 40-41 0.0 0.0 0.0 0.21053011563935692 0.0 42-43 0.0 0.0 0.0 0.2715474812085371 0.0 44-45 0.0 0.0 0.0 0.3530386648053452 0.0 46-47 0.0 0.0 0.0 0.4501815434995897 0.0 48-49 0.0 0.0 0.0 0.5576960273788826 0.0 50-51 0.0 0.0 0.0 0.6760400834254205 0.0 52-53 0.0 0.0 0.0 0.7887269002801142 0.0 54-55 0.0 0.0 0.0 0.9145331242130377 0.0 56-57 0.0 0.0 0.0 1.0679790024832587 0.0 58-59 0.0 0.0 0.0 1.2396358031043695 0.0 60-61 0.0 0.0 0.0 1.4317125432846147 0.0 62-63 0.0 0.0 0.0 1.6177010165250651 0.0 64-65 0.0 0.0 0.0 1.8124178016615042 0.0 66-67 0.0 0.0 0.0 2.0353399650544253 0.0 68-69 0.0 0.0 0.0 2.3019036879272896 0.0 70-71 0.0 0.0 0.0 2.59586461202923 0.0 72-73 0.0 0.0 0.0 2.8971260686120743 0.0 74-75 0.0 0.0 0.0 3.199707547673016 0.0 76-77 0.0 0.0 0.0 3.535774494902827 0.0 78-79 0.0 0.0 0.0 3.92154432931813 0.0 80-81 0.0 0.0 0.0 4.355939481549049 0.0 82-83 0.0 0.0 0.0 4.802080139911607 0.0 84-85 0.0 0.0 0.0 5.23997739259462 0.0 86-87 0.0 0.0 0.0 5.699830121188839 0.0 88-89 0.0 0.0 0.0 6.20653017813838 0.0 90-91 0.0 0.0 0.0 6.76718952128442 0.0 92-93 0.0 0.0 0.0 7.359152254625332 0.0 94-95 0.0 0.0 0.0 7.947963097559359 0.0 96-97 0.0 0.0 0.0 8.53424165247418 0.0 98-99 0.0 0.0 0.0 9.16567036398677 0.0 100-101 0.0 0.0 0.0 9.862345900845298 0.0 102-103 0.0 0.0 0.0 10.60225890867007 0.0 104-105 0.0 0.0 0.0 11.330049261083744 0.0 106-107 0.0 0.0 0.0 12.041541376778056 0.0 108-109 0.0 0.0 0.0 12.764213274684824 0.0 110-111 0.0 0.0 0.0 13.558785988796512 0.0 112-113 0.0 0.0 0.0 14.413756466089701 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2020 0.0 63.048004 1 GTATCAA 3715 0.0 47.578037 1 TCAACGC 4075 0.0 41.339172 4 CAACGCA 4095 0.0 40.994133 5 ATCAACG 4180 0.0 40.153996 3 GTACATG 9095 0.0 39.391518 1 AACGCAG 4325 0.0 38.538822 6 TACATGG 9115 0.0 38.06662 2 ACATGGG 9310 0.0 36.44023 3 TATCAAC 5075 0.0 33.89177 2 CGCAGAG 5295 0.0 30.805952 8 ACGCAGA 5350 0.0 30.60053 7 CATGGGG 3315 0.0 29.08938 4 CATGGGA 6320 0.0 29.009258 4 GCAGAGT 6370 0.0 25.513687 9 GAGTACA 6260 0.0 25.097965 1 CATTCCG 330 2.910383E-11 23.451975 9 AGTACAT 6455 0.0 23.234684 2 ATGGGAT 3045 0.0 22.286612 5 CAGAGTA 6075 0.0 22.048866 10-11 >>END_MODULE