FastQCFastQC Report
Thu 26 May 2016
SRR1364128_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364128_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856029
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82620.4451439066954234No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76090.40996126676899985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46410.25004997227952797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.11966407852463512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44500.073.398011
GTATCAA80200.052.8395271
ATCAACG102450.040.939693
TCAACGC102750.040.820164
CAACGCA105000.039.9454425
TATCAAC108000.038.8368952
AACGCAG109050.038.790296
TGGTATC18450.036.1160962
GTACATG91150.035.0486951
TACATGG92050.034.3848342
ACGCAGA125400.033.5958027
GTGGTAT20450.033.2257841
CGCAGAG126500.033.2095878
ACATGGG90400.033.1029743
GCAGAGT140900.029.6466399
CATGGGA61400.029.165244
GAGTACT98250.027.01131612-13
CAGAGTA139850.026.27385510-11
CATGGGG31200.025.7422544
AGAGTAC134450.025.2047410-11
Produced by FastQC (version 0.11.5)