FastQCFastQC Report
Thu 26 May 2016
SRR1364127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020198
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42010.41178281078770984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.059.8223081
GTATCAA33200.050.179831
GTACATG83650.040.8988531
ATCAACG39750.039.374353
TACATGG87050.039.09832
TCAACGC40200.038.79324
CAACGCA40850.038.321645
AACGCAG41350.037.5703626
ACATGGG89050.036.4214633
TATCAAC45550.036.3150832
CATGGGA64200.034.9531824
ATGGGAG23200.032.5833785
ACGCAGA49350.031.2387037
CGCAGAG49400.030.9661038
ATGGGAT25300.028.4672325
CATGGGG23300.028.3561634
GAGTACA57950.027.310991
AGTACAT55950.027.0124662
GCAGAGT52150.026.7080369
GTGTACG1150.00100483625.8691061
Produced by FastQC (version 0.11.5)