##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364127_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1020198 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.81446738770317 31.0 31.0 34.0 27.0 34.0 2 30.833285303441098 33.0 31.0 34.0 27.0 34.0 3 30.908293292086437 33.0 31.0 34.0 27.0 34.0 4 34.23143154564114 37.0 35.0 37.0 30.0 37.0 5 34.38159945422359 37.0 35.0 37.0 31.0 37.0 6 34.3022276067979 37.0 35.0 37.0 30.0 37.0 7 34.23710691454012 37.0 35.0 37.0 30.0 37.0 8 34.125694227983196 37.0 35.0 37.0 30.0 37.0 9 35.47545770526898 39.0 37.0 39.0 28.0 39.0 10-11 35.432726784408516 39.0 36.0 39.0 28.5 39.0 12-13 35.30939190235621 39.0 35.0 39.0 27.5 39.0 14-15 36.53219571102864 40.0 37.0 41.0 27.0 41.0 16-17 36.60019231560932 40.0 37.0 41.0 28.0 41.0 18-19 36.63508260161262 40.0 37.0 41.0 28.5 41.0 20-21 36.68612661463755 40.0 37.0 41.0 29.0 41.0 22-23 36.68165836435672 40.0 37.0 41.0 29.0 41.0 24-25 36.73381000550874 40.0 37.0 41.0 30.0 41.0 26-27 36.74210398373649 40.0 37.0 41.0 30.0 41.0 28-29 36.72262835253549 40.0 37.0 41.0 30.0 41.0 30-31 36.68195487542614 40.0 36.5 41.0 30.0 41.0 32-33 36.596385701599104 40.0 36.0 41.0 30.0 41.0 34-35 36.566661569616876 40.0 36.0 41.0 30.0 41.0 36-37 36.50109292509885 40.0 36.0 41.0 30.0 41.0 38-39 36.4061035210812 40.0 36.0 41.0 30.0 41.0 40-41 36.319062084026825 40.0 36.0 41.0 29.5 41.0 42-43 36.185197383253055 40.0 35.5 41.0 29.0 41.0 44-45 36.07695712008845 40.0 35.0 41.0 29.0 41.0 46-47 35.95722987106424 40.0 35.0 41.0 28.0 41.0 48-49 35.837442339624275 40.0 35.0 41.0 28.0 41.0 50-51 35.711182045054 40.0 35.0 41.0 28.0 41.0 52-53 35.57147975196972 39.0 35.0 41.0 27.0 41.0 54-55 35.39831238641911 39.0 35.0 41.0 27.0 41.0 56-57 35.22027243731119 39.0 35.0 41.0 27.0 41.0 58-59 35.009432482714146 39.0 34.0 41.0 26.5 41.0 60-61 34.78278579256184 38.0 34.0 40.5 26.0 41.0 62-63 34.523847331596414 38.0 34.0 40.0 26.0 41.0 64-65 34.2404430316468 37.0 34.0 40.0 26.0 41.0 66-67 33.9409746931478 37.0 33.5 40.0 25.5 41.0 68-69 33.61116763608633 36.0 33.0 39.5 25.0 41.0 70-71 33.263639019092366 36.0 33.0 39.0 24.5 41.0 72-73 32.90513900242894 35.0 33.0 39.0 24.0 40.5 74-75 32.62381175026809 35.0 33.0 37.5 23.5 40.0 76-77 32.381797455003834 35.0 33.0 37.0 23.5 39.0 78-79 32.06619695392463 35.0 33.0 37.0 22.5 39.0 80-81 31.840780417134713 35.0 32.5 36.0 22.5 39.0 82-83 31.71918637362551 35.0 33.0 36.0 23.0 37.0 84-85 31.49506517362316 35.0 33.0 36.0 22.5 37.0 86-87 31.28874689030953 35.0 33.0 35.0 20.5 37.0 88-89 31.101486182094064 35.0 33.0 35.0 20.0 36.0 90-91 30.951143307475608 35.0 32.5 35.0 19.5 36.0 92-93 30.8228157671354 35.0 32.0 35.0 19.0 36.0 94-95 30.709479434384306 35.0 32.0 35.0 18.0 36.0 96-97 30.620460440032232 35.0 32.0 35.0 16.5 35.0 98-99 30.538338636225518 35.0 32.0 35.0 13.5 35.0 100-101 30.468481118371137 35.0 32.0 35.0 9.0 35.0 102-103 29.405306126849887 33.5 30.0 35.0 7.0 35.0 104-105 30.19886286779625 35.0 31.5 35.0 6.0 35.0 106-107 30.289621720489553 35.0 32.0 35.0 2.0 35.0 108-109 30.26386054471779 35.0 32.0 35.0 2.0 35.0 110-111 30.192594966859374 35.0 32.0 35.0 2.0 35.0 112-113 30.116372998182705 35.0 32.0 35.0 2.0 35.0 114-115 30.01782693163484 35.0 32.0 35.0 2.0 35.0 116-117 29.92429214721064 35.0 32.0 35.0 2.0 35.0 118-119 29.799398254064407 35.0 32.0 35.0 2.0 35.0 120-121 29.662463560995022 35.0 32.0 35.0 2.0 35.0 122-123 29.515679309310546 35.0 31.0 35.0 2.0 35.0 124-125 28.70932848329442 34.5 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5970683984228629 1101 2 0.5582063371591346 1101 3 0.5691369220534135 1101 4 0.5991389763086303 1101 5 0.5784403604469617 1101 6 0.545763147431181 1101 7 0.47476001004507395 1101 8 0.5228628728070746 1101 9 0.583663867962656 1101 10-11 0.6230645022956196 1101 12-13 0.5660155178502038 1101 14-15 0.7712441186234784 1101 16-17 0.6315268466074571 1101 18-19 0.614875699363175 1101 20-21 0.5002682630363111 1101 22-23 0.6458186691307688 1101 24-25 0.6734414751278166 1101 26-27 0.6263936065289784 1101 28-29 0.6693548412997288 1101 30-31 0.6074379993694592 1101 32-33 0.6453845204138347 1101 34-35 0.6194850815085715 1101 36-37 0.6261821579347071 1101 38-39 0.6073790564977344 1101 40-41 0.7318825804648199 1101 42-43 0.7065695108123293 1101 44-45 0.7110443009847955 1101 46-47 0.6418681306964231 1101 48-49 0.5189044865648498 1101 50-51 0.589285259349623 1101 52-53 0.7272171346969429 1101 54-55 0.604981125858977 1101 56-57 0.628876339069194 1101 58-59 0.5320217824470603 1101 60-61 0.5431357364316938 1101 62-63 0.6479799344545825 1101 64-65 0.7190524396159859 1101 66-67 0.7251561334246119 1101 68-69 0.6586467224198742 1101 70-71 0.7066179370682946 1101 72-73 0.7450655635898045 1101 74-75 0.7759661491545131 1101 76-77 0.7199919420820962 1101 78-79 0.703265419741399 1101 80-81 0.6287315566710525 1101 82-83 0.7578231666342354 1101 84-85 0.780346179569694 1101 86-87 0.7637760109861418 1101 88-89 0.722406118492497 1101 90-91 0.7518893821566017 1101 92-93 0.7361551406180027 1101 94-95 0.6204855141677719 1101 96-97 0.6086535200313072 1101 98-99 0.5988097433118398 1101 100-101 0.5737928809085169 1101 102-103 0.5957963891369147 1101 104-105 0.6608582662731095 1101 106-107 0.6404919223808996 1101 108-109 0.6473688972243821 1101 110-111 0.6011080444224426 1101 112-113 0.6769022658741335 1101 114-115 0.6124527204294132 1101 116-117 0.6739609303279295 1101 118-119 0.58437995028412 1101 120-121 0.6136595877914068 1101 122-123 0.6352601110918386 1101 124-125 0.5888451379712016 1102 1 0.13517812612181146 1102 2 0.10767073930773563 1102 3 0.13020128694303423 1102 4 0.11731514919030417 1102 5 0.04817054332645654 1102 6 0.14494655476499219 1102 7 0.09786368315960914 1102 8 0.07048606031289495 1102 9 0.16923222266814975 1102 10-11 0.10197747152591319 1102 12-13 0.2159177130251706 1102 14-15 0.24471882909014653 1102 16-17 0.26111047749515137 1102 18-19 0.11976128324244684 1102 20-21 0.167548667917238 1102 22-23 0.2572338894244979 1102 24-25 0.26093177226818653 1102 26-27 0.2781525409221288 1102 28-29 0.26181455502656803 1102 30-31 0.2143976665225651 1102 32-33 0.3501738519256179 1102 34-35 0.44217735337493025 1102 36-37 0.35516498775430705 1102 38-39 0.41368048165375626 1102 40-41 0.49848145648556397 1102 42-43 0.2509635866343558 1102 44-45 0.33828993081682057 1102 46-47 0.42019737589156847 1102 48-49 0.3964016145184033 1102 50-51 0.33718944167024034 1102 52-53 0.40766548443457395 1102 54-55 0.28709526253733486 1102 56-57 0.3941168147421834 1102 58-59 0.48530212522968696 1102 60-61 0.3762496557660029 1102 62-63 0.3616275886912419 1102 64-65 0.310754554063152 1102 66-67 0.2284939171362339 1102 68-69 0.24954621631871987 1102 70-71 0.3793381531703872 1102 72-73 0.3597968331895842 1102 74-75 0.2729683536442167 1102 76-77 0.30139194829192917 1102 78-79 0.335739726569102 1102 80-81 0.3144396325551817 1102 82-83 0.387346251677851 1102 84-85 0.3117989693358929 1102 86-87 0.3587646780461 1102 88-89 0.34504560680248986 1102 90-91 0.3125982342694975 1102 92-93 0.19622593268183763 1102 94-95 0.3154943631757554 1102 96-97 0.38087694972050556 1102 98-99 0.3455557923074011 1102 100-101 0.2804187696904563 1102 102-103 0.3255321608635562 1102 104-105 0.4039063614077065 1102 106-107 0.35961509436214456 1102 108-109 0.3220498671998513 1102 110-111 0.38359942828309457 1102 112-113 0.4095473430312708 1102 114-115 0.5259513387421997 1102 116-117 0.4953118788794839 1102 118-119 0.4180403346726038 1102 120-121 0.527527690998788 1102 122-123 0.5763474523530547 1102 124-125 0.579337887994491 1103 1 0.3036987801265596 1103 2 0.3408114362074741 1103 3 0.24632409322278903 1103 4 0.23962297940759214 1103 5 0.3012757821373526 1103 6 0.28415784469130756 1103 7 0.3180371666180335 1103 8 0.36625477264421136 1103 9 0.3569427412371695 1103 10-11 0.39200825941703954 1103 12-13 0.4369404641921619 1103 14-15 0.47275152490091443 1103 16-17 0.5450197649911956 1103 18-19 0.44104476867077125 1103 20-21 0.4151603376567934 1103 22-23 0.39884424442591637 1103 24-25 0.40067272016339217 1103 26-27 0.47227650257781306 1103 28-29 0.578125718569801 1103 30-31 0.4295016614229752 1103 32-33 0.17004653923707025 1103 34-35 0.5943071627379055 1103 36-37 0.46123093271680204 1103 38-39 0.5242869212145393 1103 40-41 0.5361267914892451 1103 42-43 0.5500966120149968 1103 44-45 0.6297189006508859 1103 46-47 0.5865865605104617 1103 48-49 0.6316768795133854 1103 50-51 0.49466142601733054 1103 52-53 0.4193960914938799 1103 54-55 0.4106578487201915 1103 56-57 0.49116440870501066 1103 58-59 0.4671660431935649 1103 60-61 0.6076650355460345 1103 62-63 0.6205809774619624 1103 64-65 0.495205009020097 1103 66-67 0.5521828863948244 1103 68-69 0.5760742673854509 1103 70-71 0.4824180489652221 1103 72-73 0.48052874834692716 1103 74-75 0.44832409654872407 1103 76-77 0.5351647819067367 1103 78-79 0.4894671419577392 1103 80-81 0.5221332852916696 1103 82-83 0.3520743067286425 1103 84-85 0.3226997862884886 1103 86-87 0.2736145787767299 1103 88-89 0.26268567400021325 1103 90-91 0.290397088189696 1103 92-93 0.3159323963682823 1103 94-95 0.2690022128706424 1103 96-97 0.31100326639532483 1103 98-99 0.4222663352234193 1103 100-101 0.3068199950086843 1103 102-103 0.3022546559443562 1103 104-105 0.3063195920114481 1103 106-107 0.3047958265871742 1103 108-109 0.2657361361545476 1103 110-111 0.28131634347363743 1103 112-113 0.22486005814257126 1103 114-115 0.3592830489420429 1103 116-117 0.34626506402073076 1103 118-119 0.3517233057151685 1103 120-121 0.29792859759261603 1103 122-123 0.28116373208683143 1103 124-125 0.2713972238525315 1104 1 0.3435131666034934 1104 2 0.38502905341709237 1104 3 0.39090911518312765 1104 4 0.2970391255693059 1104 5 0.40957177069189754 1104 6 0.27990464798419623 1104 7 0.20162460519785697 1104 8 0.30981831294710105 1104 9 0.34748211355910286 1104 10-11 0.45190554862369936 1104 12-13 0.2784708201836068 1104 14-15 0.2632513346791967 1104 16-17 0.2972066448728796 1104 18-19 0.26255696875900725 1104 20-21 0.2991918501605184 1104 22-23 0.3327605075784561 1104 24-25 0.384803272985188 1104 26-27 0.48273378485432517 1104 28-29 0.36933461773086407 1104 30-31 0.3307388616451661 1104 32-33 -0.2822531340816994 1104 34-35 0.21270499581790858 1104 36-37 0.3478199448677799 1104 38-39 0.28461939840133255 1104 40-41 0.2862786116341738 1104 42-43 0.34479447206540215 1104 44-45 0.31368428208560317 1104 46-47 0.3161008878668383 1104 48-49 0.36639595866169117 1104 50-51 0.38289165858792984 1104 52-53 0.4028731478977434 1104 54-55 0.39644123173741264 1104 56-57 0.39691334641052833 1104 58-59 0.47015645351876856 1104 60-61 0.41061766944693545 1104 62-63 0.486285342559043 1104 64-65 0.5052387577452464 1104 66-67 0.4277470768451579 1104 68-69 0.47994867283097165 1104 70-71 0.6329236100478184 1104 72-73 0.5866470108814639 1104 74-75 0.44458641853960756 1104 76-77 0.5235114694590877 1104 78-79 0.5087764317431009 1104 80-81 0.5151310276368086 1104 82-83 0.5283044517793947 1104 84-85 0.5798464963459544 1104 86-87 0.48258607767023065 1104 88-89 0.46140127557414345 1104 90-91 0.32856489588894533 1104 92-93 0.3574746300534244 1104 94-95 0.39371129868117194 1104 96-97 0.3891803708457964 1104 98-99 0.36488434859430896 1104 100-101 0.402514465004554 1104 102-103 0.3147724501584044 1104 104-105 0.43654533070575496 1104 106-107 0.4402638255798017 1104 108-109 0.4544476832375466 1104 110-111 0.49857097086234603 1104 112-113 0.39642686882999456 1104 114-115 0.38692681815399155 1104 116-117 0.43685837714710374 1104 118-119 0.47810330617955543 1104 120-121 0.48452046753455136 1104 122-123 0.6000706538351572 1104 124-125 0.6967396675853053 1105 1 -0.09946504872342388 1105 2 -0.030015116582468693 1105 3 -0.0746631903003987 1105 4 -0.0676325660757513 1105 5 -0.013586713903542602 1105 6 -0.04559272232594225 1105 7 0.023529042707927772 1105 8 0.06415107492720296 1105 9 -0.14687211079226614 1105 10-11 -0.04423415456562907 1105 12-13 -0.04527403397481322 1105 14-15 -0.11280164002010906 1105 16-17 -0.11977042042253316 1105 18-19 0.09194485167554234 1105 20-21 -0.07284242370812422 1105 22-23 -0.02993433852242333 1105 24-25 0.07792249535879847 1105 26-27 0.0673572411018526 1105 28-29 0.008673386244900883 1105 30-31 0.10181483697442673 1105 32-33 -0.3176394933896347 1105 34-35 0.0036904336356258227 1105 36-37 -0.07244697964034685 1105 38-39 0.05513042991366035 1105 40-41 0.10294381329256197 1105 42-43 -0.006900908763498137 1105 44-45 -0.14987089012611676 1105 46-47 -0.12287693923288145 1105 48-49 -0.09590113769190367 1105 50-51 -0.13839399605101477 1105 52-53 -0.11582951402229469 1105 54-55 -0.07498378181391274 1105 56-57 -0.13877150833389607 1105 58-59 -0.19137352787562634 1105 60-61 -0.22295903838646325 1105 62-63 -0.09683373331372991 1105 64-65 -0.21370489803639714 1105 66-67 -0.23374261682160835 1105 68-69 -0.2906923781659714 1105 70-71 -0.23855121378493038 1105 72-73 -0.2918488651202438 1105 74-75 -0.1778397450432152 1105 76-77 -0.17083927549762024 1105 78-79 -0.2790931165948436 1105 80-81 -0.2667292398737011 1105 82-83 -0.1258318159655154 1105 84-85 -0.18788559759038392 1105 86-87 -0.21803215582279023 1105 88-89 -0.3091573687071971 1105 90-91 -0.300752300378214 1105 92-93 -0.21508631429091452 1105 94-95 -0.36196011461231237 1105 96-97 -0.19649606010103682 1105 98-99 -0.24062188501591208 1105 100-101 -0.3657120692253635 1105 102-103 -0.26233001984555315 1105 104-105 -0.1866994062520071 1105 106-107 -0.16781532881741867 1105 108-109 -0.12409141050833128 1105 110-111 -0.17490523740323027 1105 112-113 -0.1615490024836781 1105 114-115 -0.1978248263178024 1105 116-117 -0.17357303116535405 1105 118-119 -0.177368865988214 1105 120-121 -0.17982507664277492 1105 122-123 -0.17611179097065488 1105 124-125 -0.12002625339214745 1106 1 -0.0028822206674306017 1106 2 -0.04667322828886 1106 3 -0.02511660319486353 1106 4 0.1126912926390844 1106 5 -0.0036081547727278007 1106 6 -0.06098496725445557 1106 7 -0.11763715878277026 1106 8 -0.23521639269761607 1106 9 -0.24255800473214606 1106 10-11 -0.028063942937293973 1106 12-13 -0.09998968848274359 1106 14-15 -0.03932522497620994 1106 16-17 -0.10942760712384825 1106 18-19 -0.09271463417134385 1106 20-21 -0.05531879063037337 1106 22-23 -0.0016702201645486525 1106 24-25 0.04647766950571253 1106 26-27 0.07126378350772455 1106 28-29 0.013294401504815312 1106 30-31 0.013746867815058295 1106 32-33 0.1026837504374285 1106 34-35 0.04757336969800008 1106 36-37 -0.03716044193970447 1106 38-39 0.027743194983756325 1106 40-41 -0.03057238231659909 1106 42-43 -0.0024550028466734375 1106 44-45 6.877220454484245E-4 1106 46-47 0.038754999641930965 1106 48-49 0.10022600505831036 1106 50-51 -0.0623918626577975 1106 52-53 0.0027382252225152115 1106 54-55 -0.010864354568504098 1106 56-57 0.1431846100667471 1106 58-59 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-1.2132669373805598 2216 92-93 -1.286422184704655 2216 94-95 -1.324040520545175 2216 96-97 -1.228487668219696 2216 98-99 -1.2984087702618154 2216 100-101 -1.2495576976406646 2216 102-103 -1.4401793563014458 2216 104-105 -1.346598898835925 2216 106-107 -1.426895149270191 2216 108-109 -1.3319368437636 2216 110-111 -1.449553949347024 2216 112-113 -1.368394240578919 2216 114-115 -1.4002252947018725 2216 116-117 -1.5236803691825287 2216 118-119 -1.5691135966673713 2216 120-121 -1.5939839369081916 2216 122-123 -1.7062591367942304 2216 124-125 -1.936463607022123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22250.0 3 4196.0 4 2622.0 5 2567.0 6 3510.0 7 3350.0 8 3137.0 9 3366.0 10 3701.0 11 3973.0 12 4317.0 13 4325.0 14 4453.0 15 4054.0 16 3621.0 17 3466.0 18 3215.0 19 3175.0 20 3167.0 21 3389.0 22 4125.0 23 5605.0 24 7574.0 25 8129.0 26 10224.0 27 10864.0 28 12815.0 29 16534.0 30 19346.0 31 22064.0 32 29084.0 33 38937.0 34 55044.0 35 83459.0 36 150115.0 37 301860.0 38 153709.0 39 856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.4963201802634 19.313513944709637 12.358048971689072 22.832116903337887 2 15.717761940541761 21.337037368939868 39.67506639789102 23.270134292627347 3 18.830980839335197 27.6811440072948 28.752124531519875 24.73575062185013 4 12.138509757737904 16.861453808910024 37.419612762701696 33.580423670650376 5 12.812265803724687 40.82236190975153 33.09340473744905 13.271967549074738 6 30.67515491780907 37.06164098087187 18.202805615961484 14.06039848535757 7 28.661609568222058 34.18438276829339 20.567361910964458 16.586645752520095 8 26.70064876710601 35.667705902755884 19.99333890395409 17.63830642618402 9 28.032235844343205 16.74052569495975 19.484790164480458 35.74244829621659 10-11 24.6122291887502 28.422645667537445 27.07592595604139 19.889199187670968 12-13 24.820271351201633 26.36706881264998 27.264985108655317 21.547674727493074 14-15 22.36704852127219 27.650665367155447 24.99919930267886 24.983086808893507 16-17 21.925889355814753 31.160034036481317 25.424384369824992 21.489692237878938 18-19 21.766090827471952 29.934719171217306 27.228481915219678 21.070708086091063 20-21 23.089912998230005 29.084539939213084 26.679425875043854 21.14612118751306 22-23 22.652794903107594 29.323591361540107 26.439636538899386 21.583977196452913 24-25 22.175402528362024 29.412585041723787 26.913198554376933 21.498813875537255 26-27 21.878500578326957 29.732165870183707 26.784694319582442 21.60463923190689 28-29 21.646404255549786 29.535342526230107 27.065174438714624 21.753078779505483 30-31 21.787438899400822 29.283644749290445 27.241899243140967 21.68701710816777 32-33 21.558245865943178 29.563279809088595 26.88724824740825 21.99122607755998 34-35 22.014408908077552 29.52229655584622 26.900192614806357 21.56310192126987 36-37 22.317057134231238 29.10335697529931 26.65505664073947 21.924529249729982 38-39 21.797195294933207 29.326907217662267 27.012826136940465 21.86307135046406 40-41 21.80552150199349 29.30899004191788 27.23549736745451 21.649991088634117 42-43 21.817079734083492 29.28882942867146 26.792649233355476 22.101441603889576 44-45 21.64933071558256 29.504355777739367 26.884832261562348 21.961481245115724 46-47 21.752978944377443 29.524703435173333 26.654270095224774 22.068047525224447 48-49 21.770250682989126 29.520792075306844 26.72805975927065 21.980897482433377 50-51 21.738753618135636 29.44367132801705 26.58949290456395 22.228082149283367 52-53 21.960245347053018 29.44711230694382 26.750037916913698 21.842604429089462 54-55 21.654050413150706 29.282148797278946 26.815307699049423 22.24849309052092 56-57 21.727388236521946 29.61619556928718 26.564043792192372 22.092372401998503 58-59 21.731143065914825 29.176965755488638 26.752305846993785 22.339585331602756 60-61 21.765437770093584 29.45383304554534 26.807512915993968 21.973216268367104 62-63 21.726067952654937 29.351142557177102 26.78967138554632 22.133118104621637 64-65 21.465434374950757 29.22024456735845 26.75408531492775 22.560235742763044 66-67 21.6404128512205 29.694242283663343 26.36289854729873 22.30244631781743 68-69 21.57056371380502 29.33930984755653 26.774829688060237 22.315296750578213 70-71 21.477501010850204 29.306135676043315 26.865320278221432 22.351043034885045 72-73 21.74123128906577 29.369332538677885 26.602059865138433 22.287376307117913 74-75 21.440979105359716 29.47136683206718 26.691751726855216 22.395902335717892 76-77 21.438284212304495 29.386035979021514 26.84439642032031 22.33128338835368 78-79 21.630159974032974 29.18369849876888 26.774360219616433 22.411781307581712 80-81 21.47016785904608 29.59878723045872 26.621828078688175 22.30921683180703 82-83 21.737747097089404 29.434794343510756 26.647822866306537 22.1796356930933 84-85 21.64515852588217 29.033872243515297 26.701615548509306 22.61935368209323 86-87 21.648308191899947 28.933274696527445 26.81836067446773 22.600056437104886 88-89 21.865398016749527 28.795102696127316 26.9300494212031 22.409449865920053 90-91 21.745718160357615 28.976219060009278 26.98206507234145 22.295997707291658 92-93 21.885136236434338 28.815410501725697 26.791991810007136 22.507461451832825 94-95 21.67600158550006 28.565344021507656 26.93882776397714 22.819826629015143 96-97 21.93643614886937 28.571238669445425 26.746189177120243 22.746136004564963 98-99 21.690700238203043 28.659517294328406 26.68641848928086 22.963363978187687 100-101 21.459955746986147 28.710717576005734 26.95673913557013 22.872587541437987 102-103 21.837842891694326 28.520125088267932 26.95115800144181 22.69087401859593 104-105 21.522665681141646 28.537002215954978 26.923336225278423 23.016995877624957 106-107 21.460785900964453 28.654880399421494 26.99214965118036 22.892184048433695 108-109 21.644191997117886 28.785737874432225 26.50170313355904 23.068366994890845 110-111 21.44688417483356 29.254316786880985 26.491701175363254 22.807097862922202 112-113 21.445477566033798 29.18975437435758 26.68865167787085 22.67611638173777 114-115 21.346770950292665 29.434396368023602 26.342018330198556 22.876814351485177 116-117 21.284613559645727 29.579759465969957 26.445748914919232 22.689878059465084 118-119 21.319990171375558 29.325690379618308 26.361788908131413 22.992530540874718 120-121 21.207895528568777 29.58129787662535 26.45510226892796 22.75570432587791 122-123 21.454036943314108 29.5506150574006 26.367112801295928 22.628235197989365 124-125 21.53570709504712 29.668509891931834 26.079463306886915 22.716319706134136 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6369.0 1 3813.0 2 1850.5 3 2401.5 4 2090.0 5 1586.5 6 1251.0 7 1087.5 8 1061.0 9 1054.5 10 897.5 11 751.0 12 707.0 13 683.5 14 696.0 15 716.5 16 762.0 17 853.0 18 936.5 19 1112.0 20 1343.0 21 1640.5 22 2132.5 23 2811.0 24 3626.0 25 4466.0 26 5850.5 27 7639.5 28 8756.5 29 10152.5 30 12411.5 31 14802.0 32 17692.5 33 20629.5 34 24015.5 35 26406.5 36 28739.0 37 31539.5 38 31918.5 39 31843.0 40 31695.5 41 32228.5 42 32974.0 43 34461.0 44 36516.5 45 37657.0 46 37001.0 47 35474.0 48 35657.5 49 35600.0 50 34487.5 51 32670.0 52 31281.0 53 30521.0 54 29397.5 55 27550.0 56 24672.0 57 22039.5 58 20625.0 59 18705.0 60 16383.0 61 14101.0 62 11863.0 63 9209.5 64 6900.5 65 5567.5 66 4270.5 67 3395.5 68 2494.5 69 1604.0 70 1293.0 71 1176.5 72 1052.5 73 1001.0 74 803.0 75 681.0 76 513.0 77 275.0 78 162.0 79 94.0 80 50.5 81 32.5 82 28.5 83 18.0 84 20.5 85 18.0 86 11.0 87 12.0 88 10.0 89 6.0 90 4.5 91 6.0 92 6.0 93 3.0 94 1.5 95 1.0 96 0.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4700068026010637 2 0.4635374701773577 3 0.45873448095369723 4 0.4553037743653683 5 0.4662820354480209 6 0.5199970986024282 7 0.5150960891905296 8 0.5244080070731367 9 0.5319555615674604 10-11 0.5473937412149407 12-13 0.5569997196622617 14-15 0.5349451773087185 16-17 0.5297501073321061 18-19 0.539601136250022 20-21 0.53969915643826 22-23 0.5507754377091506 24-25 0.5439140245324927 26-27 0.5521967304386011 28-29 0.5321516019439363 30-31 0.5369545911675969 32-33 0.5377877627676196 34-35 0.5370036012617159 36-37 0.48848360808392094 38-39 0.456234966153629 40-41 0.4549116936124164 42-43 0.4672132272362816 44-45 0.4727023577776079 46-47 0.47672118549536463 48-49 0.48926776958982465 50-51 0.4731434486246787 52-53 0.472457307307013 54-55 0.4615770664125983 56-57 0.4447666041297866 58-59 0.4642236114950235 60-61 0.4600577534949098 62-63 0.47480979182472416 64-65 0.4757899937071039 66-67 0.48196526556609603 68-69 0.4902969815663234 70-71 0.4865232043191616 72-73 0.4982366168135989 74-75 0.48867964846039685 76-77 0.4869152850721135 78-79 0.49642324333119653 80-81 0.4901989613780854 82-83 0.49108114307222717 84-85 0.4901989613780854 86-87 0.4811320939660733 88-89 0.4847588409308781 90-91 0.47098700448344344 92-93 0.4717221558952282 94-95 0.46515480328328423 96-97 0.45447060276534557 98-99 0.4173699615172741 100-101 0.4138412347407072 102-103 0.40340208469336347 104-105 0.4075189325993582 106-107 0.4046763471404571 108-109 0.41918333499967647 110-111 0.44226708932971837 112-113 0.44167896820029057 114-115 0.4354546862471794 116-117 0.4288383235411165 118-119 0.4701538328834206 120-121 0.46682114648332973 122-123 0.4311417979647088 124-125 0.44074777641202983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1020198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.65679609437308 #Duplication Level Percentage of deduplicated Percentage of total 1 83.3264641934571 51.37642812041064 2 9.714047903931093 11.978741417273033 3 2.858622713681474 5.2876055330460625 4 1.233431878731755 3.0419783137305334 5 0.6602550770519695 2.0354606328033933 6 0.42306618862179707 1.565094343576663 7 0.26417942771966646 1.1401919975067754 8 0.182603294102174 0.9006987256494865 9 0.15014121098626365 0.8331503428028073 >10 1.046655256264408 12.787844339987457 >50 0.10177537546456582 4.305952368783241 >100 0.0375740454843013 3.666215854558236 >500 8.875758775818868E-4 0.31430440428916717 >1k 2.95858625860629E-4 0.7663336055825835 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4201 0.41178281078770984 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.96040376475939E-4 0.0 5 0.0 0.0 0.0 1.96040376475939E-4 0.0 6 0.0 0.0 0.0 1.96040376475939E-4 0.0 7 0.0 0.0 0.0 1.96040376475939E-4 0.0 8 0.0 0.0 0.0 1.96040376475939E-4 0.0 9 0.0 0.0 0.0 1.96040376475939E-4 0.0 10-11 0.0 0.0 0.0 1.96040376475939E-4 0.0 12-13 0.0 0.0 0.0 1.96040376475939E-4 0.0 14-15 0.0 0.0 0.0 2.450504705949237E-4 0.0 16-17 0.0 0.0 0.0 3.92080752951878E-4 0.0 18-19 0.0 0.0 0.0 5.881211294278169E-4 0.0 20-21 0.0 0.0 0.0 5.881211294278169E-4 0.0 22-23 0.0 0.0 0.0 7.84161505903756E-4 0.0 24-25 0.0 0.0 0.0 0.0014703028235695424 0.0 26-27 0.0 0.0 0.0 0.0020094138588783745 0.0 28-29 0.0 0.0 0.0 0.005685170917802231 0.0 30-31 0.0 0.0 0.0 0.01597729068278903 0.0 32-33 0.0 0.0 0.0 0.036316479742167694 0.0 34-35 0.0 0.0 0.0 0.0652324352723687 0.0 36-37 0.0 0.0 0.0 0.11257618619130796 0.0 38-39 0.0 0.0 0.0 0.18359181256971685 0.0 40-41 0.0 0.0 0.0 0.2624980641012823 0.0 42-43 0.0 0.0 0.0 0.3354740942444506 0.0 44-45 0.0 0.0 0.0 0.42486850591747877 0.0 46-47 0.0 0.0 0.0 0.5345040864616477 0.0 48-49 0.0 0.0 0.0 0.6578624933591322 0.0 50-51 0.0 0.0 0.0 0.800187806680664 0.0 52-53 0.0 0.0 0.0 0.9386903326609148 0.0 54-55 0.0 0.0 0.0 1.0850344737002033 0.0 56-57 0.0 0.0 0.0 1.2610297216814774 0.0 58-59 0.0 0.0 0.0 1.4660879554753097 0.0 60-61 0.0 0.0 0.0 1.6926126104932573 0.0 62-63 0.0 0.0 0.0 1.9225189620054146 0.0 64-65 0.0 0.0 0.0 2.143554486482036 0.0 66-67 0.0 0.0 0.0 2.403160955030298 0.0 68-69 0.0 0.0 0.0 2.7131988104269955 0.0 70-71 0.0 0.0 0.0 3.067982881754326 0.0 72-73 0.0 0.0 0.0 3.4339412545407852 0.0 74-75 0.0 0.0 0.0 3.774855469232443 0.0 76-77 0.0 0.0 0.0 4.14767525519556 0.0 78-79 0.0 0.0 0.0 4.5873938196310915 0.0 80-81 0.0 4.901009411898475E-5 0.0 5.081513588538695 0.0 82-83 0.0 9.80201882379695E-5 0.0 5.5902873755878755 0.0 84-85 0.0 9.80201882379695E-5 0.0 6.078574943295321 0.0 86-87 0.0 9.80201882379695E-5 0.0 6.5721556011676165 0.0 88-89 0.0 9.80201882379695E-5 0.0 7.127439967535714 0.0 90-91 0.0 9.80201882379695E-5 0.0 7.755357293388146 0.0 92-93 0.0 9.80201882379695E-5 0.0 8.43154956194778 0.0 94-95 0.0 9.80201882379695E-5 0.0 9.07789468318895 0.0 96-97 0.0 9.80201882379695E-5 0.0 9.71992691614765 0.0 98-99 0.0 9.80201882379695E-5 0.0 10.388326579742365 0.0 100-101 0.0 9.80201882379695E-5 0.0 11.169400449716623 0.0 102-103 0.0 9.80201882379695E-5 0.0 11.989829425268429 0.0 104-105 0.0 9.80201882379695E-5 0.0 12.794379130325682 0.0 106-107 0.0 9.80201882379695E-5 0.0 13.588195624770878 0.0 108-109 0.0 9.80201882379695E-5 0.0 14.371867029733444 0.0 110-111 0.0 9.80201882379695E-5 0.0 15.224740687592018 0.0 112-113 0.0 9.80201882379695E-5 0.0 16.148237891076047 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1840 0.0 59.822308 1 GTATCAA 3320 0.0 50.17983 1 GTACATG 8365 0.0 40.898853 1 ATCAACG 3975 0.0 39.37435 3 TACATGG 8705 0.0 39.0983 2 TCAACGC 4020 0.0 38.7932 4 CAACGCA 4085 0.0 38.32164 5 AACGCAG 4135 0.0 37.570362 6 ACATGGG 8905 0.0 36.421463 3 TATCAAC 4555 0.0 36.315083 2 CATGGGA 6420 0.0 34.953182 4 ATGGGAG 2320 0.0 32.583378 5 ACGCAGA 4935 0.0 31.238703 7 CGCAGAG 4940 0.0 30.966103 8 ATGGGAT 2530 0.0 28.467232 5 CATGGGG 2330 0.0 28.356163 4 GAGTACA 5795 0.0 27.31099 1 AGTACAT 5595 0.0 27.012466 2 GCAGAGT 5215 0.0 26.708036 9 GTGTACG 115 0.001004836 25.869106 1 >>END_MODULE