FastQCFastQC Report
Thu 26 May 2016
SRR1364127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020198
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99800.9782414786149355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88480.8672826255295542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58000.568517091780223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.23926727948888354No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.22348602918257046No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.14477581802748093No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13250.12987674941530958No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11300.11076281270890552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37550.084.1058351
GTATCAA69000.053.5430151
ATCAACG93550.039.4299663
TCAACGC94050.039.2836044
GTGGTAT12750.038.7907451
TATCAAC95350.038.6232152
CAACGCA96400.038.2642485
AACGCAG98550.038.0331656
TGGTATC13450.037.1565632
GTACATG70400.036.396191
ATACCGT700.002790050833.996996
TACATGG74300.033.871162
ACATGGG71600.033.8189353
ACGCAGA113200.032.9565857
CGCAGAG113850.032.7161678
ATGGGTA4550.031.3818365
CATGGGG20200.030.630954
GCAGAGT121300.030.4124899
CATGGGA50500.029.9240824
GAGTACT89950.028.2123312-13
Produced by FastQC (version 0.11.5)