##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364127_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1020198 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08688411465226 33.0 31.0 34.0 30.0 34.0 2 32.094930591904706 33.0 31.0 34.0 30.0 34.0 3 32.22456327105131 34.0 31.0 34.0 30.0 34.0 4 35.86961256540397 37.0 35.0 37.0 35.0 37.0 5 35.822875559450225 37.0 35.0 37.0 35.0 37.0 6 35.87525068663142 37.0 35.0 37.0 35.0 37.0 7 35.80150911881811 37.0 35.0 37.0 33.0 37.0 8 35.78702369540031 37.0 35.0 37.0 33.0 37.0 9 37.427692467540616 39.0 37.0 39.0 34.0 39.0 10-11 37.35763302809846 39.0 37.0 39.0 34.0 39.0 12-13 37.30333082401651 39.0 37.0 39.0 33.5 39.0 14-15 38.537481449679376 40.0 38.0 41.0 34.0 41.0 16-17 38.53867582567305 40.0 38.0 41.0 34.0 41.0 18-19 38.73026608560299 40.0 38.0 41.0 34.0 41.0 20-21 38.80212909650872 40.0 38.0 41.0 34.0 41.0 22-23 38.75087286977626 40.0 38.0 41.0 34.0 41.0 24-25 38.701474125610915 40.0 38.0 41.0 34.0 41.0 26-27 38.557353082440855 40.0 38.0 41.0 34.0 41.0 28-29 38.4494416770078 40.0 38.0 41.0 34.0 41.0 30-31 38.22372372813905 40.0 37.5 41.0 34.0 41.0 32-33 37.96760383768641 40.0 36.5 41.0 33.0 41.0 34-35 37.784293833157875 40.0 36.0 41.0 33.0 41.0 36-37 37.6114278796861 40.0 36.0 41.0 33.0 41.0 38-39 37.46257785253451 40.0 36.0 41.0 33.0 41.0 40-41 37.27314452684675 40.0 36.0 41.0 32.5 41.0 42-43 37.04752214766153 40.0 35.0 41.0 31.5 41.0 44-45 36.748302290339716 40.0 35.0 41.0 31.0 41.0 46-47 36.27146250041659 40.0 35.0 41.0 30.0 41.0 48-49 35.816829674239706 40.0 35.0 41.0 27.5 41.0 50-51 35.58922434664643 39.5 35.0 41.0 27.0 41.0 52-53 35.44269543755232 39.0 35.0 41.0 26.5 41.0 54-55 35.269003173893694 39.0 35.0 41.0 26.0 41.0 56-57 35.08480608666161 39.0 35.0 41.0 26.0 41.0 58-59 34.88689597509503 38.5 35.0 41.0 26.0 41.0 60-61 34.6676047198681 38.0 34.5 41.0 26.0 41.0 62-63 34.42135595247197 38.0 34.0 40.0 25.0 41.0 64-65 34.20530965557666 37.0 34.0 40.0 25.0 41.0 66-67 34.00142178283039 37.0 34.0 40.0 25.0 41.0 68-69 33.68442351386692 36.0 34.0 39.5 23.5 41.0 70-71 33.380786866863104 36.0 34.0 39.0 24.0 41.0 72-73 33.048950301804155 35.5 34.0 39.0 23.0 40.5 74-75 32.76303570483377 35.0 33.5 37.5 22.5 40.0 76-77 32.55809999627523 35.0 33.5 37.0 23.0 39.0 78-79 32.26224811262128 35.0 33.0 37.0 22.5 39.0 80-81 31.9737374509654 35.0 33.0 36.0 22.0 39.0 82-83 31.709829366456315 35.0 33.0 36.0 21.0 37.0 84-85 31.49152321412118 35.0 33.0 35.5 20.0 37.0 86-87 31.29313917494447 35.0 33.0 35.0 20.0 36.5 88-89 31.134056820342717 35.0 33.0 35.0 20.0 36.0 90-91 30.98681971538858 35.0 33.0 35.0 19.0 36.0 92-93 30.870674614143528 35.0 33.0 35.0 18.0 36.0 94-95 30.765169114230766 35.0 33.0 35.0 17.5 35.5 96-97 30.68596243082225 35.0 33.0 35.0 16.5 35.0 98-99 30.619660105195265 35.0 33.0 35.0 15.0 35.0 100-101 30.55268144026944 35.0 33.0 35.0 10.5 35.0 102-103 29.383224628944575 33.5 30.0 34.5 7.0 35.0 104-105 30.27445064585502 35.0 32.0 35.0 7.0 35.0 106-107 30.402099396391684 35.0 32.5 35.0 6.0 35.0 108-109 30.381424488187587 35.0 33.0 35.0 2.0 35.0 110-111 30.325836749336894 35.0 33.0 35.0 2.0 35.0 112-113 30.25643404515594 35.0 33.0 35.0 2.0 35.0 114-115 30.167575803912573 35.0 32.0 35.0 2.0 35.0 116-117 30.085661802904923 35.0 32.0 35.0 2.0 35.0 118-119 29.991780517115306 35.0 32.0 35.0 2.0 35.0 120-121 29.864029335481934 35.0 32.0 35.0 2.0 35.0 122-123 29.709750460204784 35.0 32.0 35.0 2.0 35.0 124-125 28.87749534894207 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2570811056282096 1101 2 0.27830917576899594 1101 3 0.23558017992139213 1101 4 0.11766233025570472 1101 5 0.07982498755065137 1101 6 0.15798914490827087 1101 7 0.17645384422819177 1101 8 0.16703664598794887 1101 9 0.20715667665970727 1101 10-11 0.21269416343604775 1101 12-13 0.21056031878429593 1101 14-15 0.3067776796163528 1101 16-17 0.3092626020717546 1101 18-19 0.31590007761394645 1101 20-21 0.301392980167428 1101 22-23 0.39047648838620574 1101 24-25 0.2815262176346991 1101 26-27 0.3906978307000699 1101 28-29 0.3887597924769608 1101 30-31 0.42047692672863235 1101 32-33 0.4632708867872566 1101 34-35 0.447141450202281 1101 36-37 0.416998521722995 1101 38-39 0.4265980724169154 1101 40-41 0.3583702614162263 1101 42-43 0.2823941097443594 1101 44-45 0.39619313018657465 1101 46-47 0.4530497726855671 1101 48-49 0.32776715698249603 1101 50-51 0.35711910922958623 1101 52-53 0.42057777126017015 1101 54-55 0.43575160968135407 1101 56-57 0.3743816893073415 1101 58-59 0.4716750620392318 1101 60-61 0.44872469522879044 1101 62-63 0.32654135964760655 1101 64-65 0.38130742827367925 1101 66-67 0.3169099075352051 1101 68-69 0.33255240059376945 1101 70-71 0.33473488310941235 1101 72-73 0.4550557690072523 1101 74-75 0.4918284708096792 1101 76-77 0.39350082828700295 1101 78-79 0.3080899342716421 1101 80-81 0.33823456529601614 1101 82-83 0.3386534069493976 1101 84-85 0.39892459486279463 1101 86-87 0.3908423016856837 1101 88-89 0.3210842129705007 1101 90-91 0.35282429547548944 1101 92-93 0.30127621436261975 1101 94-95 0.31726964243935285 1101 96-97 0.3301844597487822 1101 98-99 0.31068337679232627 1101 100-101 0.32794047702896734 1101 102-103 0.2317910226835309 1101 104-105 0.1990189245812921 1101 106-107 0.21112615566004322 1101 108-109 0.28118956305465304 1101 110-111 0.34796114288714364 1101 112-113 0.2717319586985525 1101 114-115 0.27323108740142743 1101 116-117 0.25572779590478945 1101 118-119 0.1689057780061347 1101 120-121 0.14137348774680447 1101 122-123 0.14677848650170233 1101 124-125 0.24745207277550563 1102 1 0.08729192936218055 1102 2 0.1568759469678085 1102 3 0.16559849892036738 1102 4 0.06057846649937915 1102 5 0.07955485993864642 1102 6 0.12122384543921783 1102 7 0.1612149210127427 1102 8 0.28808176040406863 1102 9 0.2525753744583241 1102 10-11 0.20195736708742373 1102 12-13 0.2987104103792859 1102 14-15 0.4193401660323488 1102 16-17 0.3217490180682745 1102 18-19 0.2078769770457427 1102 20-21 0.30804370827001293 1102 22-23 0.37372547152651237 1102 24-25 0.3648124597559743 1102 26-27 0.34795401724336017 1102 28-29 0.22464950589329646 1102 30-31 0.35593816186385396 1102 32-33 0.4183458704864549 1102 34-35 0.558930812763208 1102 36-37 0.6191657524492413 1102 38-39 0.5399833613292699 1102 40-41 0.5199019160254679 1102 42-43 0.5766205201520407 1102 44-45 0.413546500571897 1102 46-47 0.5149260728687537 1102 48-49 0.40203517325225846 1102 50-51 0.3439294299673179 1102 52-53 0.35833509109747297 1102 54-55 0.34189623662580715 1102 56-57 0.24318505503529764 1102 58-59 0.33126614472311644 1102 60-61 0.23327619024539814 1102 62-63 0.2985800468895121 1102 64-65 0.35307598790357275 1102 66-67 0.257736435929651 1102 68-69 0.26880073170191565 1102 70-71 0.45073606297713553 1102 72-73 0.3834020912353324 1102 74-75 0.29370694443342416 1102 76-77 0.24699851512527005 1102 78-79 0.3288354243824969 1102 80-81 0.2824407937556721 1102 82-83 0.31459497244757983 1102 84-85 0.31049506091054724 1102 86-87 0.3339587049520567 1102 88-89 0.290556687898313 1102 90-91 0.23117993244881418 1102 92-93 0.21026494352087965 1102 94-95 0.2744932273743039 1102 96-97 0.26144474477998614 1102 98-99 0.2799028011410698 1102 100-101 0.2034007806896625 1102 102-103 0.2622797363172147 1102 104-105 0.41991034570092367 1102 106-107 0.33069643541289295 1102 108-109 0.25498407977461923 1102 110-111 0.26922825873152334 1102 112-113 0.34720468201956933 1102 114-115 0.4398749846908352 1102 116-117 0.3473910989134552 1102 118-119 0.3616682209533195 1102 120-121 0.3847320743894578 1102 122-123 0.38752428710405695 1102 124-125 0.40180671617600794 1103 1 0.3163179828680356 1103 2 0.36746202358118296 1103 3 0.26061422487050834 1103 4 0.2535970742492495 1103 5 0.2385553933593343 1103 6 0.17588242299880363 1103 7 0.2131147618718785 1103 8 0.24370160188295387 1103 9 0.3297478464094681 1103 10-11 0.32543075470719174 1103 12-13 0.32772008499267713 1103 14-15 0.2550711626436879 1103 16-17 0.3691156684298278 1103 18-19 0.3786939960430473 1103 20-21 0.2518096583232463 1103 22-23 0.3791382528173912 1103 24-25 0.3544335539987955 1103 26-27 0.4153967630540194 1103 28-29 0.4716609113572048 1103 30-31 0.5100940844935096 1103 32-33 0.4933616407038741 1103 34-35 0.4779522403386238 1103 36-37 0.576490769436802 1103 38-39 0.5603598102525709 1103 40-41 0.5579815130161734 1103 42-43 0.6931614308627303 1103 44-45 0.7270535534557965 1103 46-47 0.7014909543099179 1103 48-49 0.6230293720256057 1103 50-51 0.5778242199301573 1103 52-53 0.5698567566095107 1103 54-55 0.5449876419126483 1103 56-57 0.5414272014670161 1103 58-59 0.5929326748990107 1103 60-61 0.4638336701978574 1103 62-63 0.5389478194265394 1103 64-65 0.4647789279147716 1103 66-67 0.5588278456432221 1103 68-69 0.5197762718180243 1103 70-71 0.4792333438596543 1103 72-73 0.5586994013342519 1103 74-75 0.5716306320746654 1103 76-77 0.4995769733402824 1103 78-79 0.462907283453184 1103 80-81 0.4146208907452724 1103 82-83 0.45285119587338585 1103 84-85 0.47646724778043037 1103 86-87 0.44915300509747524 1103 88-89 0.40052346530695004 1103 90-91 0.36988679675411973 1103 92-93 0.3762818403404964 1103 94-95 0.3568018101352983 1103 96-97 0.4121431717422972 1103 98-99 0.40714532605801423 1103 100-101 0.4644878522100875 1103 102-103 0.4254863250587171 1103 104-105 0.4766266876302261 1103 106-107 0.5184317436570076 1103 108-109 0.5527401715052704 1103 110-111 0.5751926102933851 1103 112-113 0.5019365219225342 1103 114-115 0.6518736301121599 1103 116-117 0.5582746516280359 1103 118-119 0.5220819871911075 1103 120-121 0.5291877086178047 1103 122-123 0.6001873843526262 1103 124-125 0.711610368669028 1104 1 0.20558706710678365 1104 2 0.19879386133724353 1104 3 0.12421811360955815 1104 4 0.09683384401667894 1104 5 0.14382721393292286 1104 6 0.0904853775974388 1104 7 0.08794441073867887 1104 8 0.15420273338956036 1104 9 0.21920849194872716 1104 10-11 0.19631665204462934 1104 12-13 0.09748148623902608 1104 14-15 0.10350888869941599 1104 16-17 0.181126606624737 1104 18-19 0.29009086640471793 1104 20-21 0.21918730468232894 1104 22-23 0.29800824654811464 1104 24-25 0.25245931162597657 1104 26-27 0.2595676732425787 1104 28-29 0.2460860934967286 1104 30-31 0.3634858358371176 1104 32-33 0.40641268085047244 1104 34-35 0.40123394886895625 1104 36-37 0.32263141204682455 1104 38-39 0.39204529964034407 1104 40-41 0.35955459298252634 1104 42-43 0.6408904247595615 1104 44-45 0.4818211525362983 1104 46-47 0.33554278392128367 1104 48-49 0.4015760245700193 1104 50-51 0.35674595753850724 1104 52-53 0.45500437044336195 1104 54-55 0.464286081022955 1104 56-57 0.41830218025685895 1104 58-59 0.35202670158006555 1104 60-61 0.4451341169063028 1104 62-63 0.4353589251594414 1104 64-65 0.36114560392879724 1104 66-67 0.3358525012347542 1104 68-69 0.40624806988651585 1104 70-71 0.18876680055429773 1104 72-73 0.32795242836002814 1104 74-75 0.2300549533046592 1104 76-77 0.22162375895058517 1104 78-79 0.3387456432240654 1104 80-81 0.43152427776340474 1104 82-83 0.3813331908972515 1104 84-85 0.34947215246416974 1104 86-87 0.3975120525957472 1104 88-89 0.44609987281365093 1104 90-91 0.41467306971244966 1104 92-93 0.35176757549828963 1104 94-95 0.38082489215568316 1104 96-97 0.416283619665311 1104 98-99 0.4295984091679337 1104 100-101 0.36586593259617217 1104 102-103 0.3966882762154995 1104 104-105 0.4497705970525381 1104 106-107 0.3877137058162816 1104 108-109 0.36886052379945156 1104 110-111 0.2392822788432163 1104 112-113 0.36618631607228025 1104 114-115 0.4078256495880055 1104 116-117 0.36950416260641816 1104 118-119 0.2970994911607576 1104 120-121 0.4475274481658822 1104 122-123 0.5022520043646637 1104 124-125 0.6744606490004621 1105 1 0.1370686299776125 1105 2 0.17133594370000793 1105 3 0.1140894126324028 1105 4 0.06818743841449049 1105 5 0.16460469382568732 1105 6 0.18368004789732595 1105 7 0.2650346112983115 1105 8 0.2088127481861406 1105 9 0.297020575337001 1105 10-11 0.1831178430963618 1105 12-13 0.21781635050601267 1105 14-15 0.23068259473085817 1105 16-17 0.2878506283654616 1105 18-19 0.2809217458491844 1105 20-21 0.30126439341788114 1105 22-23 0.3166230079715149 1105 24-25 0.3346334571598959 1105 26-27 0.24942157529212494 1105 28-29 0.28361960556107135 1105 30-31 0.2831768289662264 1105 32-33 0.18466170295744888 1105 34-35 0.3136264538866129 1105 36-37 0.3433356415257194 1105 38-39 0.29945860777553435 1105 40-41 0.23565609608101568 1105 42-43 0.14826399454523198 1105 44-45 0.10643200971264832 1105 46-47 0.015334093492178624 1105 48-49 0.22498667371519332 1105 50-51 0.28255976963654916 1105 52-53 0.3430951350311773 1105 54-55 0.3408627961813906 1105 56-57 0.1399780764756713 1105 58-59 0.057908892939444456 1105 60-61 -0.017707779400410573 1105 62-63 -0.0066670347872275215 1105 64-65 -0.031348426162473686 1105 66-67 0.005425378071507225 1105 68-69 4.068987588965456E-4 1105 70-71 -0.010969681081824945 1105 72-73 0.059712159677509646 1105 74-75 0.19102621716729828 1105 76-77 0.07690474753817256 1105 78-79 0.0651599255897608 1105 80-81 0.17844409786038185 1105 82-83 0.17540888054990234 1105 84-85 0.18312046257712566 1105 86-87 0.13359538959160133 1105 88-89 0.09348794618126632 1105 90-91 0.08136079587310263 1105 92-93 0.07635883818892708 1105 94-95 0.07433142609260202 1105 96-97 0.0687589342758379 1105 98-99 -3.000291332426741E-4 1105 100-101 0.012581017786406079 1105 102-103 0.08526414166913554 1105 104-105 0.00800467607508537 1105 106-107 -0.032278529639846454 1105 108-109 0.07084945566191436 1105 110-111 0.03192500727462999 1105 112-113 0.002319463349561346 1105 114-115 0.07385870017473906 1105 116-117 0.11326963675634616 1105 118-119 0.07999533639728185 1105 120-121 0.18629358156953302 1105 122-123 0.25475442162831996 1105 124-125 0.40754895969941884 1106 1 0.030947021217336612 1106 2 0.0925394127364001 1106 3 0.08319130220925075 1106 4 -0.004683445847597056 1106 5 0.017181340922881816 1106 6 -0.0720557856231494 1106 7 -0.022941392038127617 1106 8 0.059968469850510076 1106 9 0.030232802513971535 1106 10-11 0.04681393347951257 1106 12-13 0.07705049442182599 1106 14-15 0.17503124144307947 1106 16-17 0.1210436068818197 1106 18-19 0.21683038956717127 1106 20-21 0.12007969182558753 1106 22-23 0.17028640236587478 1106 24-25 0.11142117651442618 1106 26-27 0.11173684161872899 1106 28-29 0.09265417829427491 1106 30-31 0.05931958451893138 1106 32-33 -0.029593266939784257 1106 34-35 -0.02466875640500632 1106 36-37 -0.07529866600371093 1106 38-39 -0.1143515041875034 1106 40-41 -0.2182600706551554 1106 42-43 -0.10504637406661743 1106 44-45 -0.15300183905704046 1106 46-47 -0.10395012367548873 1106 48-49 -0.09926035610953932 1106 50-51 -0.20925553946486275 1106 52-53 -0.16349017931014487 1106 54-55 -0.21967023131211505 1106 56-57 -0.061857497187887134 1106 58-59 -0.022830549704892178 1106 60-61 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-0.8837130252299126 2216 80-81 -0.9589144111169752 2216 82-83 -0.8862909633220752 2216 84-85 -0.8934847286797059 2216 86-87 -0.9614524246160521 2216 88-89 -1.0340003442168602 2216 90-91 -0.9870232790683495 2216 92-93 -0.9822184451834381 2216 94-95 -1.0382905489269163 2216 96-97 -1.2042114013994158 2216 98-99 -1.209223721561031 2216 100-101 -1.2549394740168722 2216 102-103 -1.2458736413129046 2216 104-105 -1.2498017251739348 2216 106-107 -1.4434660784298572 2216 108-109 -1.445846485009433 2216 110-111 -1.392108950570801 2216 112-113 -1.287049193949425 2216 114-115 -1.5373190906137069 2216 116-117 -1.5670192000898666 2216 118-119 -1.6493713693013916 2216 120-121 -1.9139113364632543 2216 122-123 -1.8797791458970465 2216 124-125 -2.290704358021113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 29.0 5 117.0 6 333.0 7 649.0 8 1277.0 9 2223.0 10 3872.0 11 5551.0 12 6967.0 13 10096.0 14 15523.0 15 14458.0 16 5786.0 17 4459.0 18 4135.0 19 3753.0 20 3656.0 21 3511.0 22 3775.0 23 4022.0 24 4913.0 25 6250.0 26 7934.0 27 9980.0 28 12490.0 29 15444.0 30 19623.0 31 24717.0 32 31658.0 33 40619.0 34 55647.0 35 84777.0 36 148852.0 37 304792.0 38 157325.0 39 971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.07131860881586 19.44590624901819 13.677778126865437 21.80499701530051 2 16.39596386534194 21.478674435199924 39.03776181551563 23.087599883942506 3 18.931521136093192 27.21128643655447 30.51809550695061 23.339096920401726 4 12.215961999533425 17.318304878072688 37.26845181033486 33.197281312059026 5 11.890927055336316 39.708762416707344 33.90028210210175 14.50002842585459 6 30.017310365242828 37.72267736262961 17.242633292752977 15.017378979374593 7 26.707266628634834 34.999186432437625 21.085514772622567 17.208032166304974 8 26.912422882616905 34.158369257732325 19.910742816590503 19.018465043060267 9 27.786986238932283 14.620607588534371 20.537209970231242 37.055196202302106 10-11 24.657772584852943 27.270419328135944 27.101088374094473 20.970719712916644 12-13 26.0120465806346 25.22473994570265 28.345734609584035 20.41747886407871 14-15 22.800524996128203 25.668693724159432 27.9429581316568 23.587823148055573 16-17 20.588741253497496 29.24224669143648 28.298014053216374 21.87099800184965 18-19 20.31257657827206 26.036857551181242 33.15679897431675 20.49376689622995 20-21 21.463382598279942 25.11551679183845 33.4997716129614 19.921328996920206 22-23 21.0512567168334 25.27411345640748 33.387146416675975 20.28748341008314 24-25 20.730387630636407 25.417124911046677 33.74839982042702 20.1040876378899 26-27 20.48499408938265 25.72794692794928 33.59985022515237 20.187208757515698 28-29 20.25944963624708 25.498481667284196 33.885039962830746 20.35702873363798 30-31 20.38785608283882 25.27475058763103 34.00055675466919 20.336836574860957 32-33 20.324976132084164 25.45339238069473 33.61818000035287 20.603451486868234 34-35 20.72146779350675 25.40786200325819 33.51359245950296 20.3570777437321 36-37 21.147806602247798 25.05126455844846 33.10597550671536 20.69495333258838 38-39 20.65721556011676 25.246961864265565 33.39655635474683 20.69926622087085 40-41 20.702304846706227 25.280680809019422 33.38200035679348 20.63501398748086 42-43 20.978427716972586 25.40992042721119 32.64219298606741 20.969458869748813 44-45 20.837523696380504 25.667713522277047 32.401406393660835 21.09335638768161 46-47 21.236220812038447 25.89154262211845 31.48849537050651 21.38374119533659 48-49 21.415401716137456 26.21907708111563 30.76476331065146 21.60075789209546 50-51 21.496513421904375 26.41497042730921 30.1633604457174 21.925155705069017 52-53 21.6044826592485 26.589593392655154 30.16105697129381 21.644866976802543 54-55 21.256412970815468 26.54965016594818 30.122584047410406 22.071352815825946 56-57 21.412461110490316 26.752797006071372 29.852293378344203 21.98244850509411 58-59 21.48666239298646 26.241376673939765 30.1042542722099 22.16770666086387 60-61 21.615362900142912 26.424233335097696 30.29152184183854 21.668881922920843 62-63 21.50450206724577 26.296954120670694 30.456636848925406 21.74190696315813 64-65 21.32590928427619 26.060480416546593 30.447324931042797 22.16628536813442 66-67 21.509158026187073 26.480790983711007 29.98775727848908 22.022293711612846 68-69 21.379281276771763 26.31935173368307 30.26089053301418 22.040476456530985 70-71 21.34752273578266 26.360030111801823 30.18463082656504 22.10781632585047 72-73 21.533369012681852 26.53509416799484 29.70560616664608 22.225930652677224 74-75 21.27518383686304 26.66095208969239 29.648656437279826 22.415207636164745 76-77 21.096885114458175 26.715892405199774 29.80093079970751 22.386291680634542 78-79 21.207402876696484 26.59116171566696 29.594010182337154 22.6074252252994 80-81 20.95181523586598 26.972656288289137 29.399440108684782 22.676088367160098 82-83 21.22298808662632 26.717215677740985 29.362682538095548 22.697113697537144 84-85 21.038416072174225 26.445650746227695 29.27804210555206 23.237891076046022 86-87 21.07737909699882 26.39115152156738 29.381061323390167 23.150408058043634 88-89 21.078604349351792 26.32905573231863 29.38900095863744 23.20333895969214 90-91 20.95852961876028 26.54533727766571 29.417769883885285 23.07836321968873 92-93 21.06072598687999 26.39900607480414 29.188809029624167 23.3514589086917 94-95 20.864691871122044 26.146306080239956 29.26597006439977 23.72303198423823 96-97 21.034994525212937 26.180167056976018 29.11591746550198 23.668920952309065 98-99 20.82756430394996 26.269721503527094 28.951615630463216 23.951098562059727 100-101 20.6091489480582 26.111956838185346 29.33277198402997 23.946122229726484 102-103 21.021926645209067 25.889242921271986 29.182626305994745 23.906204127524198 104-105 20.698371587131508 25.883588723552137 29.110171526939737 24.307868162376614 106-107 20.711974110032365 25.96560422506076 29.153762368763413 24.168659296143467 108-109 20.83789864013911 25.928197344793052 28.678999980880864 24.55490403418698 110-111 20.613187361552214 26.253107186221374 28.716601577722667 24.417103874503745 112-113 20.533612436086614 26.078034384544114 28.80134518376171 24.587007995607564 114-115 20.339874625639336 26.245470781361607 28.553233608407837 24.86142098459122 116-117 20.17263220232294 26.240398700282576 28.74663942253955 24.84032967485494 118-119 20.126221159077875 26.141553966285468 28.60519491978216 25.12702995485449 120-121 20.04749203523078 26.405728457131428 28.622382925613294 24.9243965820245 122-123 20.11075971083657 26.48537418900716 28.39646447317082 25.007401626985448 124-125 20.012195504525277 26.472332674540805 28.177252443022287 25.338219377911624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1650.0 1 1102.0 2 880.0 3 1161.5 4 722.5 5 477.0 6 1240.0 7 2734.5 8 4185.5 9 4604.0 10 3326.0 11 1944.5 12 1658.5 13 1644.5 14 1789.0 15 1968.5 16 2099.0 17 2324.5 18 2574.5 19 2750.5 20 2943.5 21 3308.5 22 3767.5 23 4302.5 24 4983.5 25 5722.0 26 6998.5 27 8539.5 28 9555.0 29 10777.5 30 12886.5 31 15149.5 32 17644.0 33 20218.0 34 23119.5 35 25526.0 36 27642.5 37 30016.0 38 30734.5 39 30894.0 40 30904.0 41 31440.5 42 31956.5 43 33457.0 44 35432.5 45 36451.5 46 36034.5 47 34924.0 48 35251.5 49 35094.5 50 33931.0 51 32003.5 52 30615.0 53 29961.5 54 28947.0 55 26858.5 56 23885.0 57 21373.5 58 19954.5 59 18103.5 60 15746.5 61 13473.0 62 11262.0 63 8904.5 64 6808.5 65 5361.5 66 4061.0 67 3229.0 68 2278.0 69 1393.5 70 1154.5 71 1048.0 72 939.0 73 947.5 74 789.5 75 628.5 76 424.5 77 197.0 78 104.5 79 57.5 80 28.0 81 16.5 82 8.5 83 4.0 84 3.5 85 2.5 86 1.5 87 1.0 88 2.0 89 1.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16369371435740906 2 0.001372282635331573 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 9.80201882379695E-5 10-11 6.371312235468017E-4 12-13 0.007302504023728727 14-15 0.0 16-17 4.4109084707086267E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 4.901009411898475E-5 94-95 7.84161505903756E-4 96-97 0.006567352611943957 98-99 0.002303474423592283 100-101 0.003626746964804871 102-103 0.004557938753065581 104-105 0.012252523529746186 106-107 0.018525815576976235 108-109 0.027200602236036535 110-111 0.0179867045416674 112-113 0.02577930950658598 114-115 0.028670905059606077 116-117 0.027837733459583337 118-119 0.015977290682789025 120-121 0.023279794706517757 122-123 0.014899068612171364 124-125 0.014703028235695423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1020198.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.9804324010817 #Duplication Level Percentage of deduplicated Percentage of total 1 82.69333091108025 46.29208421088008 2 10.001240396490447 11.19747523885402 3 2.945004107249092 4.945878100402972 4 1.2829890129768995 2.8728911884913546 5 0.6890301578079324 1.9286103085736805 6 0.4280743430771909 1.4378272095162081 7 0.28940224363581746 1.134060391560338 8 0.20674443265195028 0.9258914189097992 9 0.158662666201446 0.7993804193878894 >10 1.1336886944387 12.703051278975655 >50 0.1165580749043377 4.529978163688271 >100 0.050468441269748074 4.783745473835387 >500 0.0024032591080831904 0.8522822914081919 >1k 0.001502036942551994 1.4551694292729054 >5k 9.012221655311963E-4 4.141674876243281 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9980 0.9782414786149355 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8848 0.8672826255295542 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5800 0.568517091780223 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2441 0.23926727948888354 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2280 0.22348602918257046 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1477 0.14477581802748093 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1325 0.12987674941530958 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1130 0.11076281270890552 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.901009411898475E-5 0.0 16-17 0.0 0.0 0.0 1.96040376475939E-4 0.0 18-19 0.0 0.0 0.0 3.92080752951878E-4 0.0 20-21 0.0 0.0 0.0 3.92080752951878E-4 0.0 22-23 0.0 0.0 0.0 5.881211294278169E-4 0.0 24-25 0.0 0.0 0.0 0.0013722826353315728 0.0 26-27 0.0 0.0 0.0 0.0019113936706404051 0.0 28-29 0.0 0.0 0.0 0.005636160823683246 0.0 30-31 0.0 0.0 0.0 0.016075310871026996 0.0 32-33 0.0 0.0 0.0 0.036414499930405665 0.0 34-35 0.0 0.0 0.0 0.0658695664959155 0.0 36-37 0.0 0.0 0.0 0.11316430732073576 0.0 38-39 0.0 0.0 0.0 0.1843269639815016 0.0 40-41 0.0 0.0 0.0 0.26445846786604166 0.0 42-43 0.0 0.0 0.0 0.33772855857392386 0.0 44-45 0.0 0.0 0.0 0.42776010147049887 0.0 46-47 0.0 0.0 0.0 0.5378367728617386 0.0 48-49 0.0 0.0 0.0 0.6623714220180789 0.0 50-51 0.0 0.0 0.0 0.8051868362808003 0.0 52-53 0.0 0.0 0.0 0.9440324329198841 0.0 54-55 0.0 0.0 0.0 1.0914547960297902 0.0 56-57 0.0 0.0 0.0 1.2684792559875633 0.0 58-59 0.0 0.0 0.0 1.474370661381418 0.0 60-61 0.0 0.0 0.0 1.7024636394111732 0.0 62-63 0.0 0.0 0.0 1.9333992028998293 0.0 64-65 0.0 0.0 0.0 2.156444141235329 0.0 66-67 0.0 0.0 0.0 2.419334286089563 0.0 68-69 0.0 0.0 0.0 2.7327048278863515 0.0 70-71 0.0 0.0 0.0 3.0891062323196086 0.0 72-73 0.0 0.0 0.0 3.45736807952966 0.0 74-75 0.0 0.0 0.0 3.798331304315437 0.0 76-77 0.0 0.0 0.0 4.174630806961002 0.0 78-79 0.0 0.0 0.0 4.617142946761314 0.0 80-81 0.0 0.0 0.0 5.1145954020690105 0.0 82-83 0.0 0.0 0.0 5.627633067306542 0.0 84-85 0.0 0.0 0.0 6.119498371884673 0.0 86-87 0.0 0.0 0.0 6.6173429079453205 0.0 88-89 0.0 0.0 0.0 7.175518869866438 0.0 90-91 0.0 0.0 0.0 7.80877829597784 0.0 92-93 0.0 0.0 0.0 8.491145836396464 0.0 94-95 0.0 0.0 0.0 9.142980088178962 0.0 96-97 0.0 0.0 0.0 9.791971754502558 0.0 98-99 0.0 0.0 0.0 10.467379861556285 0.0 100-101 0.0 0.0 0.0 11.2584027806367 0.0 102-103 0.0 0.0 0.0 12.090251108118228 0.0 104-105 0.0 0.0 0.0 12.904406791622803 0.0 106-107 0.0 0.0 0.0 13.7077802544212 0.0 108-109 0.0 0.0 0.0 14.50292002140761 0.0 110-111 0.0 0.0 0.0 15.368928384490069 0.0 112-113 0.0 0.0 0.0 16.304678111503847 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3755 0.0 84.105835 1 GTATCAA 6900 0.0 53.543015 1 ATCAACG 9355 0.0 39.429966 3 TCAACGC 9405 0.0 39.283604 4 GTGGTAT 1275 0.0 38.790745 1 TATCAAC 9535 0.0 38.623215 2 CAACGCA 9640 0.0 38.264248 5 AACGCAG 9855 0.0 38.033165 6 TGGTATC 1345 0.0 37.156563 2 GTACATG 7040 0.0 36.39619 1 ATACCGT 70 0.0027900508 33.99699 6 TACATGG 7430 0.0 33.87116 2 ACATGGG 7160 0.0 33.818935 3 ACGCAGA 11320 0.0 32.956585 7 CGCAGAG 11385 0.0 32.716167 8 ATGGGTA 455 0.0 31.381836 5 CATGGGG 2020 0.0 30.63095 4 GCAGAGT 12130 0.0 30.412489 9 CATGGGA 5050 0.0 29.924082 4 GAGTACT 8995 0.0 28.21233 12-13 >>END_MODULE